pycom02g20330

EF hand family protein

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
pyrus_communis
Chr2
OG0005854
Physical Location & Seq
Reverse (-)
18447107 .. 18448751
1645 bp
Loading structure...
UTR
Exon/CDS
Intron
N/A

Sequence Viewer

Length: 747 bp
ATGATTACGCTGCCTGACAATGTGAAAAGAATGCTACAATACCAAAGATCCTCCCTCCTCTTACTCTTATTCTTAACTTATCATCCAATCCAAATCGCAAAAAGTTACAAGGTATCCCTATATTCCTTCGACATCGTTAATGTTGTTTTCTTCCCAGTTCTGAATTTTCCCTGTTTTCTGTTGGGCTTTAGTCTGATATGTTATGTTAGGTTACCGGACATGAAGCAACCGTTAATTACATATATGTTTATAAGGTATCTGATAAGTTTCTGGATTGCAGGTAAGCAATGGAGGCGAAAGCAAATAAAAAGGATTTCAGAGAAGTTGTTTGAACAATTTAGAAATGATAGAAGAACGGATAACTTGAAAATCAATGATCTGTACATCGGTGTACTACTTGTGTACAATGATATCAATAAGCATTTATCCGGCAAGCATTTTGATCCACCCTCGAAAGATCATGTCAGAGAAATGATGAAGGAAAGCGATTTAAATCTTGATGGAGAAATTGACCGCGAAGAGTTTGCAAAGTTCATTGAGTACTTGACAGCGGACAATTTGGTTGTTCTCGATCAACTGATCCTCACACTGATTGCAGCACCGGCGGTTGCAATGGCGACAAAGAGGGTTACCGAGGGTGTACCGGGGATCGGAAAGGTCGTGCAAAGGTTACCCGATTCAGTTTATGCATCCCTTGTGACTCTTACGGTTGTGCTGTTTCAACAAGCGCACCAGGAGTTCGGTTAG
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
No pathways identified.
Pfam Domains
Protein Families
No domains found.

Protein Analysis

249

Amino Acids

28.87

Weight (kDa)

9.08

Isoelectric Point (pI)

40.65

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
EF-hand_7 PF13499 145 - 179 7.7e-06 EF-hand domain pair
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Publication-ready
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Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AanI TTATAA 1 cut(s) 251
Acc36I ACCTGC 1 cut(s) 269
AccII CGCG 1 cut(s) 516
AciI CCGC 3 cut(s) 514, 551, 605
AclWI GGATC 4 cut(s) 42, 437, 574, 656
AcsI RAATTY 1 cut(s) 163
AfaI GTAC 5 cut(s) 383, 393, 404, 542, 642
AfiI CCNNNNNNNGG 1 cut(s) 650
AgsI TTSAA 3 cut(s) 332, 367, 722
AjnI CCWGG 1 cut(s) 732
AlwI GGATC 4 cut(s) 42, 437, 574, 656
ApeKI GCWGC 2 cut(s) 10, 596
ApoI RAATTY 1 cut(s) 163
AspLEI GCGC 1 cut(s) 730
AsuC2I CCSGG 1 cut(s) 645
BbvI GCAGC 1 cut(s) 608
BccI CCATC 1 cut(s) 494
BcgI CGANNNNNNTGC 2 cut(s) 506, 540
BciT130I CCWGG 1 cut(s) 734
BciVI GTATCC 1 cut(s) 124
BcnI CCSGG 1 cut(s) 645
BfuAI ACCTGC 1 cut(s) 269
BfuI GTATCC 1 cut(s) 124
BisI GCNGC 2 cut(s) 11, 597
BlsI GCNGC 2 cut(s) 12, 598
BmcAI AGTACT 1 cut(s) 542
Bme1390I CCNGG 2 cut(s) 645, 734
BmrFI CCNGG 2 cut(s) 645, 734
BmrI ACTGGG 1 cut(s) 149
BmsI GCATC 1 cut(s) 698
BmuI ACTGGG 1 cut(s) 149
BpuMI CCSGG 1 cut(s) 645
BsaBI GATNNNNATC 1 cut(s) 492
BsaJI CCNNGG 2 cut(s) 633, 644
BsaWI WCCGGW 1 cut(s) 214
Bsc4I CCNNNNNNNGG 1 cut(s) 650
Bse118I RCCGGY 1 cut(s) 601
Bse1I ACTGG 1 cut(s) 155
Bse3DI GCAATG 2 cut(s) 293, 618
Bse8I GATNNNNATC 1 cut(s) 492
BseBI CCWGG 1 cut(s) 734
BseDI CCNNGG 2 cut(s) 633, 644
BseGI GGATG 2 cut(s) 82, 689
BseJI GATNNNNATC 1 cut(s) 492
BseLI CCNNNNNNNGG 1 cut(s) 650
BseMI GCAATG 2 cut(s) 293, 618
BseNI ACTGG 1 cut(s) 155
BseRI GAGGAG 1 cut(s) 47
BseXI GCAGC 1 cut(s) 608
Bsh1236I CGCG 1 cut(s) 516
BsiSI CCGG 4 cut(s) 215, 429, 602, 644
BslI CCNNNNNNNGG 1 cut(s) 650
BsmI GAATGC 1 cut(s) 36
Bsp1407I TGTACA 2 cut(s) 381, 402
Bsp143I GATC 7 cut(s) 47, 376, 442, 457, 571, 579, 648
BspACI CCGC 3 cut(s) 514, 551, 605
BspFNI CGCG 1 cut(s) 516
BspMI ACCTGC 1 cut(s) 269
BspPI GGATC 4 cut(s) 42, 437, 574, 656
BsrDI GCAATG 2 cut(s) 293, 618
BsrFI RCCGGY 1 cut(s) 601
BsrGI TGTACA 2 cut(s) 381, 402
BsrI ACTGG 1 cut(s) 155
BssAI RCCGGY 1 cut(s) 601
BssECI CCNNGG 2 cut(s) 633, 644
BssMI GATC 7 cut(s) 47, 376, 442, 457, 571, 579, 648
Bst2UI CCWGG 1 cut(s) 734
Bst4CI ACNGT 2 cut(s) 231, 709
Bst6I CTCTTC 1 cut(s) 513
BstAUI TGTACA 2 cut(s) 381, 402
BstC8I GCNNGC 1 cut(s) 434
BstEII GGTNACC 3 cut(s) 210, 628, 669
BstF5I GGATG 2 cut(s) 82, 689
BstFNI CGCG 1 cut(s) 516
BstHHI GCGC 1 cut(s) 730
BstKTI GATC 7 cut(s) 50, 379, 445, 460, 574, 582, 651
BstMBI GATC 7 cut(s) 47, 376, 442, 457, 571, 579, 648
BstMWI GCNNNNNNNGC 2 cut(s) 292, 602
BstNI CCWGG 1 cut(s) 734
BstPI GGTNACC 3 cut(s) 210, 628, 669
BstSCI CCNGG 2 cut(s) 643, 732
BstUI CGCG 1 cut(s) 516
BstV1I GCAGC 1 cut(s) 608
BstX2I RGATCY 1 cut(s) 47
BstYI RGATCY 1 cut(s) 47
BsuI GTATCC 1 cut(s) 124
BtsCI GGATG 2 cut(s) 82, 689
BtsIMutI CAGTG 1 cut(s) 587
BveI ACCTGC 1 cut(s) 269
Cac8I GCNNGC 1 cut(s) 434
CfoI GCGC 1 cut(s) 730
Cfr10I RCCGGY 1 cut(s) 601
Csp6I GTAC 5 cut(s) 382, 392, 403, 541, 641
CviAII CATG 2 cut(s) 220, 461
CviJI RGCY 1 cut(s) 186
CviKI_1 RGCY 1 cut(s) 186
CviQI GTAC 5 cut(s) 382, 392, 403, 541, 641
DpnI GATC 7 cut(s) 49, 378, 444, 459, 573, 581, 650
DpnII GATC 7 cut(s) 47, 376, 442, 457, 571, 579, 648
DraI TTTAAA 1 cut(s) 492
Eam1104I CTCTTC 1 cut(s) 513
EarI CTCTTC 1 cut(s) 513
Eco32I GATATC 1 cut(s) 412
Eco91I GGTNACC 3 cut(s) 210, 628, 669
EcoO65I GGTNACC 3 cut(s) 210, 628, 669
EcoRII CCWGG 1 cut(s) 732
EcoRV GATATC 1 cut(s) 412
EcoT22I ATGCAT 1 cut(s) 691
FaeI CATG 2 cut(s) 223, 464
FatI CATG 2 cut(s) 219, 460
Fnu4HI GCNGC 2 cut(s) 11, 597
FokI GGATG 2 cut(s) 69, 676
Fsp4HI GCNGC 2 cut(s) 11, 597
GlaI GCGC 1 cut(s) 729
GluI GCNGC 2 cut(s) 11, 597
HapII CCGG 4 cut(s) 215, 429, 602, 644
HhaI GCGC 1 cut(s) 730
Hin1II CATG 2 cut(s) 223, 464
Hin6I GCGC 1 cut(s) 728
HinP1I GCGC 1 cut(s) 728
HinfI GANTC 2 cut(s) 677, 700
HpaII CCGG 4 cut(s) 215, 429, 602, 644
Hpy166II GTNNAC 3 cut(s) 392, 403, 641
Hpy188I TCNGA 6 cut(s) 162, 195, 261, 319, 467, 653
Hpy188III TCNNGA 3 cut(s) 271, 497, 569
Hpy8I GTNNAC 3 cut(s) 392, 403, 641
HpyAV CCTTC 2 cut(s) 136, 472
HpyCH4III ACNGT 2 cut(s) 231, 709
HpyCH4V TGCA 6 cut(s) 278, 527, 596, 611, 664, 689
HpyF10VI GCNNNNNNNGC 2 cut(s) 292, 602
Hsp92II CATG 2 cut(s) 223, 464
HspAI GCGC 1 cut(s) 728
Kzo9I GATC 7 cut(s) 47, 376, 442, 457, 571, 579, 648
Lsp1109I GCAGC 1 cut(s) 608
LweI GCATC 1 cut(s) 698
MaeIII GTNAC 5 cut(s) 104, 210, 628, 669, 697
MalI GATC 7 cut(s) 49, 378, 444, 459, 573, 581, 650
MboI GATC 7 cut(s) 47, 376, 442, 457, 571, 579, 648
MboII GAAGA 3 cut(s) 142, 363, 530
MflI RGATCY 1 cut(s) 47
MluCI AATT 5 cut(s) 163, 234, 335, 507, 556
MlyI GAGTC 1 cut(s) 694
MnlI CCTC 8 cut(s) 61, 65, 68, 285, 460, 593, 618, 628
Mph1103I ATGCAT 1 cut(s) 691
MseI TTAA 4 cut(s) 74, 138, 233, 491
MspA1I CMGCKG 1 cut(s) 551
MspI CCGG 4 cut(s) 215, 429, 602, 644
MspR9I CCNGG 2 cut(s) 645, 734
Mva1269I GAATGC 1 cut(s) 36
MvaI CCWGG 1 cut(s) 734
MvnI CGCG 1 cut(s) 516
MwoI GCNNNNNNNGC 2 cut(s) 292, 602
NciI CCSGG 1 cut(s) 645
NdeII GATC 7 cut(s) 47, 376, 442, 457, 571, 579, 648
NlaIII CATG 2 cut(s) 223, 464
NmuCI GTSAC 1 cut(s) 697
NsiI ATGCAT 1 cut(s) 691
PcsI WCGNNNNNNNCGW 1 cut(s) 657
PctI GAATGC 1 cut(s) 36
PfeI GAWTC 1 cut(s) 677
PkrI GCNGC 2 cut(s) 12, 598
PleI GAGTC 1 cut(s) 694
PpsI GAGTC 1 cut(s) 694
PsiI TTATAA 1 cut(s) 251
Psp6I CCWGG 1 cut(s) 732
PspEI GGTNACC 3 cut(s) 210, 628, 669
PspGI CCWGG 1 cut(s) 732
PsuI RGATCY 1 cut(s) 47
RsaI GTAC 5 cut(s) 383, 393, 404, 542, 642
RsaNI GTAC 5 cut(s) 382, 392, 403, 541, 641
SaqAI TTAA 4 cut(s) 74, 138, 233, 491
SatI GCNGC 2 cut(s) 11, 597
Sau3AI GATC 7 cut(s) 47, 376, 442, 457, 571, 579, 648
ScaI AGTACT 1 cut(s) 542
SchI GAGTC 1 cut(s) 694
ScrFI CCNGG 2 cut(s) 645, 734
SetI ASST 6 cut(s) 114, 212, 257, 283, 660, 671
SfaNI GCATC 1 cut(s) 698
SgrAI CRCCGGYG 1 cut(s) 601
SmiI ATTTAAAT 1 cut(s) 492
Sse9I AATT 5 cut(s) 163, 234, 335, 507, 556
SsiI CCGC 3 cut(s) 514, 551, 605
StyD4I CCNGG 2 cut(s) 643, 732
SwaI ATTTAAAT 1 cut(s) 492
TaaI ACNGT 2 cut(s) 231, 709
TaqI TCGA 3 cut(s) 129, 452, 570
TasI AATT 5 cut(s) 163, 234, 335, 507, 556
TatI WGTACW 4 cut(s) 381, 391, 402, 540
TfiI GAWTC 1 cut(s) 677
Tru1I TTAA 4 cut(s) 74, 138, 233, 491
Tru9I TTAA 4 cut(s) 74, 138, 233, 491
TscAI CASTG 1 cut(s) 594
TseFI GTSAC 1 cut(s) 697
TseI GCWGC 2 cut(s) 10, 596
Tsp45I GTSAC 1 cut(s) 697
TspDTI ATGAA 3 cut(s) 236, 491, 523
TspGWI ACGGA 1 cut(s) 371
TspRI CASTG 1 cut(s) 594
XapI RAATTY 1 cut(s) 163
ZrmI AGTACT 1 cut(s) 542
Zsp2I ATGCAT 1 cut(s) 691
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.