pycom04g05650

Agenet domain

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
pyrus_communis
Chr4
OG0002656
Physical Location & Seq
Forward (+)
5427254 .. 5431222
3969 bp
Loading structure...
UTR
Exon/CDS
Intron
N/A

Sequence Viewer

Length: 1884 bp
ATGGGTGATCCTTTGCCATTGAGTCAGTTAGTAGAAGCTGGTCCAGAGGGTTATTGGAAAGTACCTCAATTTTCTTCAGGACTGGTTATGAAATCCAGTGGCATGTCAAGAGAACAGTCAAATTTAGGTACTGTTGGAGAAGATGCTGATACTTCTGCTAGGGGTTCTAAAGATAGACAATCAGATAAGCATGAAGCACAGCCGACTGCACATGAGAAGTCACCTATTCTGAGAGAGGTAAATAAGGAATCAATGGATGATCAATTGAGGCGGGGTGTAGGAACCACGGGGTTTGATACAGTCATTGAAAAGGGGTCAATAGGGCCAAAAGGCCGCAATGTTTCTGAGATCATATCAAACTCTGCTTTGGTTACTGTCGACAATGATATTGAAAAGGAAGCAGAAGCATCTGAAGAAAGCAGCATCAAGGAGGGTTCTCTAGTAGAGGTTCTCAAAGATGGGGCTGGATTTGGAGAAGCCTGGTTTACTGCTAAGGTATTGTGTTTGCAGGATGGAAAAGCCTGTGTGTGTTACACTGAGCTTCAGTCAGATGAAGGGAAACTGCAGGAATGGGTGGCACTTGAAGGTGAAGAAGATAAGCCACCAAAGATACGAATTGCCCGCCCTGTTACTGCTTTGGGACACGACGGAACAAGGAAAAGGCGCAGAGCAGCGATGGCGGATTATACTTGGTCTGTTGGAGATAAGGTTGATGCATGGATACAGGAGAGCTGGTGGGAAGGAGTTGTCACTGAGAAAAACAAGAAAGATGAAACTATATTAACTGTCCACTTTCCAGCTCAAGGGGAAAAGTCAGTTGTTAAAGCTTGGCATCTTCGGCCTTCTCTCATTTGGAAGGATGGCAAATGGGTTGAATGGTTTAGTGTGCGAAATGACACCTCCTTCCTTGAGGGTGATATGCCTCAGGAAAAGCGACCCAAATTGGGCGGTCCTGCAGTGGAAGGCAAAGGGGAGGATAGCACATCAAAGGGCGTTGATGTTGTTGACTCAGGGAAACCTGTAGACCCAAGGTTACTGAATTTATCTGCAAATGAAACGGTATTTAATATGGGTACGAATACGAGAACTGAGAATAAGCTTGATGCAACCAGAACAATTCGAACAGGGTTGCAGAAGGAAGGATCAAGGGGGGTTGTTTTTGGTATTCCCAAGCCTGGAAAGAAGAGAAAATTCATGGAAGTTAGCAAACATTATGTGGAAAATGAGAGTAACAAGACTAATGAAACTAGTGATTCGGTGAAATTTCCGAAATACTTGATGCCTCAAGGATCCCGTGGACTGAAAAATACATCTAAAATTGACAAGAGGGAAAAACAAGTGGCAGAATCCAAGCTAAAGGGTCTGAGATCTGGAAAGCTGCAAAGTTTGTCAGGTAAATCCGCCGCACAGAAGGACAGCCTCTTAACAGATGCACGCAGTGCCTCCGATGGTTCAAGTGAAATGGACAATACAGGAAGGATCAAAGATTCTGTAAACCGTGCCGAGGGTTTATCCGGAAAGCATACCCTATCACAAACAACACAGGGCCAAATTGAATTTTCTTCACTAGCTCCATCATCTGATTCTTCATTTAAGAAAGTCTCCACTTTAACTGCTAAATCTCGGGCAAACAAAGGAAAACTTGCACCTGCTTTTGGAAGGTTGGGTAAAATTGAGGAGGGCAAAGTGTTTAGCGGAAATCCGGCAAAGTCGACTGCTGAAGTTGTTGAGCCTCGTCGATCAAATCGAAGAATTCAACCTACATCAAGACTACTAGAAGGACTGCAAAGCTCGTTGATCATCTCAAAGATTCCTTCTGTTTCACATGACAAGGGCCACAGAAGTCAGAACCGGAATGCCTCTAGAGGGAATAACAATGGTTAG
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
No pathways identified.
Pfam Domains
Protein Families
Agenet

Protein Analysis

628

Amino Acids

68.5

Weight (kDa)

9.2

Isoelectric Point (pI)

45.57

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
Agenet PF05641 145 - 205 6.2e-09 Agenet domain
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Publication-ready
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Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AarI CACCTGC 1 cut(s) 1657
Acc36I ACCTGC 1 cut(s) 1657
AccI GTMKAC 3 cut(s) 378, 1023, 1712
AccIII TCCGGA 1 cut(s) 1514
AciI CCGC 8 cut(s) 271, 334, 622, 680, 948, 1401, 1404, 1695
AclWI GGATC 5 cut(s) 2, 1150, 1284, 1297, 1487
AcsI RAATTY 6 cut(s) 121, 1039, 1190, 1262, 1556, 1752
AcuI CTGAAG 4 cut(s) 60, 432, 527, 1740
AdeI CACNNNGTG 1 cut(s) 1439
AfaI GTAC 3 cut(s) 63, 130, 1075
AfiI CCNNNNNNNGG 6 cut(s) 803, 944, 1175, 1504, 1655, 1866
AgsI TTSAA 7 cut(s) 308, 392, 584, 875, 1455, 1556, 1757
AhlI ACTAGT 1 cut(s) 1247
AjnI CCWGG 2 cut(s) 479, 1174
Alw26I GTCTC 1 cut(s) 1606
AlwI GGATC 5 cut(s) 2, 1150, 1284, 1297, 1487
Ama87I CYCGRG 1 cut(s) 1623
Aor13HI TCCGGA 1 cut(s) 1514
AoxI GGCC 5 cut(s) 323, 331, 839, 1546, 1834
ApeKI GCWGC 3 cut(s) 420, 671, 1378
ApoI RAATTY 6 cut(s) 121, 1039, 1190, 1262, 1556, 1752
AspLEI GCGC 1 cut(s) 666
AspS9I GGNCC 5 cut(s) 41, 323, 950, 1546, 1834
AsuHPI GGTGA 5 cut(s) 17, 213, 599, 926, 1270
AsuII TTCGAA 1 cut(s) 1120
AvaI CYCGRG 1 cut(s) 1623
AvaII GGWCC 2 cut(s) 41, 950
AxyI CCTNAGG 1 cut(s) 924
BamHI GGATCC 1 cut(s) 1289
BbvI GCAGC 3 cut(s) 432, 683, 1365
BccI CCATC 6 cut(s) 452, 506, 670, 854, 1442, 1582
BciT130I CCWGG 2 cut(s) 481, 1176
BciVI GTATCC 1 cut(s) 714
BclI TGATCA 2 cut(s) 259, 1797
BcoDI GTCTC 1 cut(s) 1606
BcuI ACTAGT 1 cut(s) 1247
BfaI CTAG 6 cut(s) 159, 440, 1248, 1568, 1775, 1863
BfmI CTRYAG 3 cut(s) 563, 954, 1020
BfuAI ACCTGC 1 cut(s) 1657
BfuI GTATCC 1 cut(s) 714
BglII AGATCT 1 cut(s) 1367
BisI GCNGC 5 cut(s) 334, 421, 672, 1379, 1404
BlsI GCNGC 5 cut(s) 335, 422, 673, 1380, 1405
Bme1390I CCNGG 2 cut(s) 481, 1176
Bme18I GGWCC 2 cut(s) 41, 950
BmeT110I CYCGRG 1 cut(s) 1623
BmgT120I GGNCC 5 cut(s) 41, 323, 950, 1546, 1834
BmiI GGNNCC 2 cut(s) 283, 1291
BmrFI CCNGG 2 cut(s) 481, 1176
BmsI GCATC 8 cut(s) 133, 416, 432, 703, 841, 1093, 1269, 1420
Bpu10I CCTNAGC 1 cut(s) 492
Bpu14I TTCGAA 1 cut(s) 1120
BpuEI CTTGAG 3 cut(s) 786, 929, 1269
BsaJI CCNNGG 4 cut(s) 285, 1028, 1294, 1503
BsaWI WCCGGW 2 cut(s) 1514, 1851
BsaXI ACNNNNNCTCC 4 cut(s) 693, 719, 723, 749
Bsc4I CCNNNNNNNGG 6 cut(s) 803, 944, 1175, 1504, 1655, 1866
Bse1I ACTGG 2 cut(s) 87, 96
Bse21I CCTNAGG 1 cut(s) 924
Bse3DI GCAATG 1 cut(s) 343
BseAI TCCGGA 1 cut(s) 1514
BseBI CCWGG 2 cut(s) 481, 1176
BseDI CCNNGG 4 cut(s) 285, 1028, 1294, 1503
BseGI GGATG 3 cut(s) 262, 517, 865
BseLI CCNNNNNNNGG 6 cut(s) 803, 944, 1175, 1504, 1655, 1866
BseMI GCAATG 1 cut(s) 343
BseMII CTCAG 8 cut(s) 221, 336, 528, 744, 938, 1023, 1080, 1355
BseNI ACTGG 2 cut(s) 87, 96
BseRI GAGGAG 1 cut(s) 1691
BseXI GCAGC 3 cut(s) 432, 683, 1365
BsgI GTGCAG 1 cut(s) 192
BshFI GGCC 5 cut(s) 325, 333, 841, 1548, 1836
BsiHKCI CYCGRG 1 cut(s) 1623
BsiSI CCGG 3 cut(s) 1515, 1703, 1852
BslFI GGGAC 1 cut(s) 654
BslI CCNNNNNNNGG 6 cut(s) 803, 944, 1175, 1504, 1655, 1866
BsmAI GTCTC 1 cut(s) 1606
BsmFI GGGAC 1 cut(s) 654
BsmI GAATGC 1 cut(s) 1861
BsnI GGCC 5 cut(s) 325, 333, 841, 1548, 1836
BsoBI CYCGRG 1 cut(s) 1623
Bsp119I TTCGAA 1 cut(s) 1120
Bsp13I TCCGGA 1 cut(s) 1514
Bsp143I GATC 9 cut(s) 7, 259, 348, 1142, 1289, 1367, 1479, 1739, 1797
BspACI CCGC 8 cut(s) 271, 334, 622, 680, 948, 1401, 1404, 1695
BspANI GGCC 5 cut(s) 325, 333, 841, 1548, 1836
BspCNI CTCAG 8 cut(s) 222, 337, 529, 745, 937, 1022, 1081, 1356
BspEI TCCGGA 1 cut(s) 1514
BspLI GGNNCC 2 cut(s) 283, 1291
BspMAI CTGCAG 2 cut(s) 567, 958
BspMI ACCTGC 1 cut(s) 1657
BspPI GGATC 5 cut(s) 2, 1150, 1284, 1297, 1487
BspT104I TTCGAA 1 cut(s) 1120
BsrDI GCAATG 1 cut(s) 343
BsrI ACTGG 2 cut(s) 87, 96
BssECI CCNNGG 4 cut(s) 285, 1028, 1294, 1503
BssMI GATC 9 cut(s) 7, 259, 348, 1142, 1289, 1367, 1479, 1739, 1797
BssT1I CCWWGG 1 cut(s) 1028
Bst2UI CCWGG 2 cut(s) 481, 1176
Bst4CI ACNGT 7 cut(s) 117, 133, 301, 376, 787, 1060, 1499
Bst6I CTCTTC 1 cut(s) 1178
BstAPI GCANNNNNTGC 1 cut(s) 1439
BstBI TTCGAA 1 cut(s) 1120
BstC8I GCNNGC 2 cut(s) 622, 1435
BstDEI CTNAG 9 cut(s) 230, 345, 492, 537, 753, 924, 1009, 1089, 1364
BstDSI CCRYGG 2 cut(s) 285, 1294
BstF5I GGATG 3 cut(s) 262, 517, 865
BstHHI GCGC 1 cut(s) 666
BstKTI GATC 9 cut(s) 10, 262, 351, 1145, 1292, 1370, 1482, 1742, 1800
BstMAI GTCTC 1 cut(s) 1606
BstMBI GATC 9 cut(s) 7, 259, 348, 1142, 1289, 1367, 1479, 1739, 1797
BstMWI GCNNNNNNNGC 3 cut(s) 677, 838, 1439
BstNI CCWGG 2 cut(s) 481, 1176
BstNSI RCATGY 1 cut(s) 106
BstSCI CCNGG 2 cut(s) 479, 1174
BstSFI CTRYAG 3 cut(s) 563, 954, 1020
BstV1I GCAGC 3 cut(s) 432, 683, 1365
BstX2I RGATCY 2 cut(s) 1289, 1367
BstYI RGATCY 2 cut(s) 1289, 1367
Bsu36I CCTNAGG 1 cut(s) 924
BsuI GTATCC 1 cut(s) 714
BsuRI GGCC 5 cut(s) 325, 333, 841, 1548, 1836
BtgI CCRYGG 2 cut(s) 285, 1294
BtgZI GCGATG 1 cut(s) 689
BtsCI GGATG 3 cut(s) 262, 517, 865
BtsI GCAGTG 2 cut(s) 963, 1444
BtsIMutI CAGTG 5 cut(s) 103, 534, 750, 963, 1444
BveI ACCTGC 1 cut(s) 1657
Cac8I GCNNGC 2 cut(s) 622, 1435
CfoI GCGC 1 cut(s) 666
Cfr13I GGNCC 5 cut(s) 41, 323, 950, 1546, 1834
Csp6I GTAC 3 cut(s) 62, 129, 1074
CspCI CAANNNNNGTGG 2 cut(s) 274, 309
CviAII CATG 6 cut(s) 103, 191, 212, 717, 1195, 1826
CviQI GTAC 3 cut(s) 62, 129, 1074
DdeI CTNAG 9 cut(s) 230, 345, 492, 537, 753, 924, 1009, 1089, 1364
DpnI GATC 9 cut(s) 9, 261, 350, 1144, 1291, 1369, 1481, 1741, 1799
DpnII GATC 9 cut(s) 7, 259, 348, 1142, 1289, 1367, 1479, 1739, 1797
DraIII CACNNNGTG 1 cut(s) 1439
Eam1104I CTCTTC 1 cut(s) 1178
EarI CTCTTC 1 cut(s) 1178
EciI GGCGGA 2 cut(s) 695, 1390
Eco130I CCWWGG 1 cut(s) 1028
Eco47I GGWCC 2 cut(s) 41, 950
Eco57I CTGAAG 4 cut(s) 60, 432, 527, 1740
Eco81I CCTNAGG 1 cut(s) 924
Eco88I CYCGRG 1 cut(s) 1623
EcoRI GAATTC 1 cut(s) 1752
EcoRII CCWGG 2 cut(s) 479, 1174
EcoT14I CCWWGG 1 cut(s) 1028
EcoT22I ATGCAT 1 cut(s) 718
ErhI CCWWGG 1 cut(s) 1028
FaeI CATG 6 cut(s) 106, 194, 215, 720, 1198, 1829
FalI AAGNNNNNCTT 2 cut(s) 1626, 1658
FaqI GGGAC 1 cut(s) 654
FatI CATG 6 cut(s) 102, 190, 211, 716, 1194, 1825
FauI CCCGC 2 cut(s) 264, 629
FbaI TGATCA 2 cut(s) 259, 1797
FblI GTMKAC 3 cut(s) 378, 1023, 1712
Fnu4HI GCNGC 5 cut(s) 334, 421, 672, 1379, 1404
FokI GGATG 3 cut(s) 269, 524, 872
Fsp4HI GCNGC 5 cut(s) 334, 421, 672, 1379, 1404
FspBI CTAG 6 cut(s) 159, 440, 1248, 1568, 1775, 1863
GlaI GCGC 1 cut(s) 665
GluI GCNGC 5 cut(s) 334, 421, 672, 1379, 1404
HaeIII GGCC 5 cut(s) 325, 333, 841, 1548, 1836
HapII CCGG 3 cut(s) 1515, 1703, 1852
HhaI GCGC 1 cut(s) 666
Hin1II CATG 6 cut(s) 106, 194, 215, 720, 1198, 1829
Hin6I GCGC 1 cut(s) 664
HinP1I GCGC 1 cut(s) 664
HincII GTYRAC 3 cut(s) 379, 1006, 1713
HindII GTYRAC 3 cut(s) 379, 1006, 1713
HindIII AAGCTT 2 cut(s) 825, 1097
HinfI GANTC 8 cut(s) 22, 248, 1007, 1253, 1346, 1487, 1583, 1810
HpaII CCGG 3 cut(s) 1515, 1703, 1852
HphI GGTGA 5 cut(s) 17, 213, 599, 926, 1270
Hpy166II GTNNAC 8 cut(s) 379, 486, 790, 1006, 1024, 1298, 1495, 1713
Hpy188III TCNNGA 8 cut(s) 44, 78, 108, 926, 1371, 1515, 1767, 1863
Hpy8I GTNNAC 8 cut(s) 379, 486, 790, 1006, 1024, 1298, 1495, 1713
Hpy99I CGWCG 2 cut(s) 650, 1740
HpyCH4III ACNGT 7 cut(s) 117, 133, 301, 376, 787, 1060, 1499
HpyF10VI GCNNNNNNNGC 3 cut(s) 677, 838, 1439
HpyF3I CTNAG 9 cut(s) 230, 345, 492, 537, 753, 924, 1009, 1089, 1364
Hsp92II CATG 6 cut(s) 106, 194, 215, 720, 1198, 1829
HspAI GCGC 1 cut(s) 664
Kpn2I TCCGGA 1 cut(s) 1514
Ksp22I TGATCA 2 cut(s) 259, 1797
Kzo9I GATC 9 cut(s) 7, 259, 348, 1142, 1289, 1367, 1479, 1739, 1797
LmnI GCTCC 1 cut(s) 1576
Lsp1109I GCAGC 3 cut(s) 432, 683, 1365
LweI GCATC 8 cut(s) 133, 416, 432, 703, 841, 1093, 1269, 1420
MaeI CTAG 6 cut(s) 159, 440, 1248, 1568, 1775, 1863
MaeIII GTNAC 7 cut(s) 219, 370, 530, 628, 748, 1032, 1229
MalI GATC 9 cut(s) 9, 261, 350, 1144, 1291, 1369, 1481, 1741, 1799
MboI GATC 9 cut(s) 7, 259, 348, 1142, 1289, 1367, 1479, 1739, 1797
MfeI CAATTG 1 cut(s) 263
MflI RGATCY 2 cut(s) 1289, 1367
MlyI GAGTC 2 cut(s) 31, 1001
MmeI TCCRAC 2 cut(s) 115, 679
Mph1103I ATGCAT 1 cut(s) 718
MroI TCCGGA 1 cut(s) 1514
MseI TTAA 6 cut(s) 782, 822, 1065, 1424, 1593, 1610
MspI CCGG 3 cut(s) 1515, 1703, 1852
MspR9I CCNGG 2 cut(s) 481, 1176
MunI CAATTG 1 cut(s) 263
Mva1269I GAATGC 1 cut(s) 1861
MvaI CCWGG 2 cut(s) 481, 1176
MwoI GCNNNNNNNGC 3 cut(s) 677, 838, 1439
NdeII GATC 9 cut(s) 7, 259, 348, 1142, 1289, 1367, 1479, 1739, 1797
NlaIII CATG 6 cut(s) 106, 194, 215, 720, 1198, 1829
NlaIV GGNNCC 2 cut(s) 283, 1291
NmeAIII GCCGAG 1 cut(s) 1528
NmuCI GTSAC 2 cut(s) 219, 748
NsiI ATGCAT 1 cut(s) 718
NspI RCATGY 1 cut(s) 106
NspV TTCGAA 1 cut(s) 1120
PaqCI CACCTGC 1 cut(s) 1657
PcsI WCGNNNNNNNCGW 1 cut(s) 1744
PctI GAATGC 1 cut(s) 1861
PfeI GAWTC 6 cut(s) 248, 1253, 1346, 1487, 1583, 1810
PkrI GCNGC 5 cut(s) 335, 422, 673, 1380, 1405
PleI GAGTC 2 cut(s) 30, 1001
PpsI GAGTC 2 cut(s) 30, 1001
Psp6I CCWGG 2 cut(s) 479, 1174
PspGI CCWGG 2 cut(s) 479, 1174
PspN4I GGNNCC 2 cut(s) 283, 1291
PspPI GGNCC 5 cut(s) 41, 323, 950, 1546, 1834
PstI CTGCAG 2 cut(s) 567, 958
PsuI RGATCY 2 cut(s) 1289, 1367
RsaI GTAC 3 cut(s) 63, 130, 1075
RsaNI GTAC 3 cut(s) 62, 129, 1074
SalI GTCGAC 2 cut(s) 377, 1711
SaqAI TTAA 6 cut(s) 782, 822, 1065, 1424, 1593, 1610
SatI GCNGC 5 cut(s) 334, 421, 672, 1379, 1404
Sau3AI GATC 9 cut(s) 7, 259, 348, 1142, 1289, 1367, 1479, 1739, 1797
Sau96I GGNCC 5 cut(s) 41, 323, 950, 1546, 1834
SchI GAGTC 2 cut(s) 31, 1001
ScrFI CCNGG 2 cut(s) 481, 1176
SfaNI GCATC 8 cut(s) 133, 416, 432, 703, 841, 1093, 1269, 1420
SfcI CTRYAG 3 cut(s) 563, 954, 1020
SfuI TTCGAA 1 cut(s) 1120
SinI GGWCC 2 cut(s) 41, 950
SmlI CTYRAG 3 cut(s) 801, 908, 1284
SmoI CTYRAG 3 cut(s) 801, 908, 1284
SpeI ACTAGT 1 cut(s) 1247
SsiI CCGC 8 cut(s) 271, 334, 622, 680, 948, 1401, 1404, 1695
SspMI CTAG 6 cut(s) 159, 440, 1248, 1568, 1775, 1863
StyD4I CCNGG 2 cut(s) 479, 1174
StyI CCWWGG 1 cut(s) 1028
TaaI ACNGT 7 cut(s) 117, 133, 301, 376, 787, 1060, 1499
TaqI TCGA 5 cut(s) 378, 1120, 1712, 1738, 1747
TauI GCSGC 2 cut(s) 336, 1406
TfiI GAWTC 6 cut(s) 248, 1253, 1346, 1487, 1583, 1810
Tru1I TTAA 6 cut(s) 782, 822, 1065, 1424, 1593, 1610
Tru9I TTAA 6 cut(s) 782, 822, 1065, 1424, 1593, 1610
TscAI CASTG 5 cut(s) 103, 541, 757, 963, 1444
TseFI GTSAC 2 cut(s) 219, 748
TseI GCWGC 3 cut(s) 420, 671, 1378
Tsp45I GTSAC 2 cut(s) 219, 748
TspDTI ATGAA 8 cut(s) 104, 207, 567, 786, 1068, 1183, 1257, 1578
TspGWI ACGGA 1 cut(s) 663
TspRI CASTG 5 cut(s) 103, 541, 757, 963, 1444
VpaK11BI GGWCC 2 cut(s) 41, 950
XapI RAATTY 6 cut(s) 121, 1039, 1190, 1262, 1556, 1752
XbaI TCTAGA 1 cut(s) 1862
XceI RCATGY 1 cut(s) 106
XcmI CCANNNNNNNNNTGG 1 cut(s) 51
XmiI GTMKAC 3 cut(s) 378, 1023, 1712
XspI CTAG 6 cut(s) 159, 440, 1248, 1568, 1775, 1863
Zsp2I ATGCAT 1 cut(s) 718
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.