pycom04g20060

No description available

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
pyrus_communis
Chr4
OG0005172
Physical Location & Seq
Forward (+)
21619398 .. 21619886
489 bp
Loading structure...
UTR
Exon/CDS
Intron
pycom04g20060.1

Sequence Viewer

Length: 489 bp
ATGGGAAAGAGTCAGCATAGCTCAAACTCCAAGTGTTTGTGCAAGAAAGTCTTCAGTTCTCCCTGCCCCATTTGTAAATTGGCAGCAGCACCAAATCATGTTTCAGTTCTGGGGAAAAGCGAGCATAAAGCAAATATGATTCCAACAACAAAGGTTCCTGCTGAGCCGAGTCATGCCAAGGCTAGTTCAAAAGTTGAGAAGCAAAAGTCCAAACTAGTGCCACAACTTACAAAAACAAATAGGACAGTAAGTCTGGGTTACAATGATGCGTTTTCCAACTACATTAATCGCGCGAGACTTAGGATCCAAGCTATGTCCAATGTTGGAATGGAGAAGATTGCCTCCAGATCAACAGATGATGAATATGATGCAAAGAAAGAAGACAACGCAAAAGATGCATTTTCAGATTATATAAATCGTGCAAAGATGAGAATCAGAAAGACATCAAGCATTGGAAGAAGAAAAATTTTCTCCTTCAAACGAGAGTAA
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
No pathways identified.
Pfam Domains
Protein Families
No domains found.

Protein Analysis

163

Amino Acids

18.1

Weight (kDa)

10.08

Isoelectric Point (pI)

42.22

Instability Index
Protein Domains (Pfam)
No Pfam domains detected for this protein.
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

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Publication-ready
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Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AccII CGCG 2 cut(s) 291, 293
AclWI GGATC 2 cut(s) 298, 311
AcsI RAATTY 1 cut(s) 465
AcuI CTGAAG 1 cut(s) 37
AgsI TTSAA 2 cut(s) 189, 478
AhdI GACNNNNNGTC 1 cut(s) 249
AhlI ACTAGT 1 cut(s) 214
AjuI GAANNNNNNNTTGG 2 cut(s) 85, 117
AluBI AGCT 2 cut(s) 21, 311
AluI AGCT 2 cut(s) 21, 311
Alw26I GTCTC 1 cut(s) 289
AlwI GGATC 2 cut(s) 298, 311
ApeKI GCWGC 2 cut(s) 83, 86
ApoI RAATTY 1 cut(s) 465
AseI ATTAAT 1 cut(s) 285
Asp700I GAANNNNTTC 1 cut(s) 50
AspLEI GCGC 1 cut(s) 293
BamHI GGATCC 1 cut(s) 303
BbsI GAAGAC 2 cut(s) 43, 387
BbvI GCAGC 2 cut(s) 95, 98
BcoDI GTCTC 1 cut(s) 289
BcuI ACTAGT 1 cut(s) 214
BfaI CTAG 2 cut(s) 183, 215
BisI GCNGC 2 cut(s) 84, 87
BlpI GCTNAGC 1 cut(s) 162
BlsI GCNGC 2 cut(s) 85, 88
BmeRI GACNNNNNGTC 1 cut(s) 249
BmiI GGNNCC 2 cut(s) 156, 305
BmsI GCATC 3 cut(s) 256, 358, 385
BpiI GAAGAC 2 cut(s) 43, 387
BpmI CTGGAG 1 cut(s) 328
Bpu1102I GCTNAGC 1 cut(s) 162
BsaBI GATNNNNATC 1 cut(s) 431
BsaJI CCNNGG 1 cut(s) 177
Bse8I GATNNNNATC 1 cut(s) 431
BseDI CCNNGG 1 cut(s) 177
BseJI GATNNNNATC 1 cut(s) 431
BseMII CTCAG 1 cut(s) 153
BseXI GCAGC 2 cut(s) 95, 98
Bsh1236I CGCG 2 cut(s) 291, 293
BsmAI GTCTC 1 cut(s) 289
Bsp143I GATC 2 cut(s) 303, 347
Bsp1720I GCTNAGC 1 cut(s) 162
BspCNI CTCAG 1 cut(s) 154
BspFNI CGCG 2 cut(s) 291, 293
BspLI GGNNCC 2 cut(s) 156, 305
BspPI GGATC 2 cut(s) 298, 311
BssECI CCNNGG 1 cut(s) 177
BssMI GATC 2 cut(s) 303, 347
BssT1I CCWWGG 1 cut(s) 177
Bst4CI ACNGT 1 cut(s) 247
BstAPI GCANNNNNTGC 1 cut(s) 395
BstC8I GCNNGC 1 cut(s) 122
BstDEI CTNAG 2 cut(s) 162, 299
BstFNI CGCG 2 cut(s) 291, 293
BstHHI GCGC 1 cut(s) 293
BstKTI GATC 2 cut(s) 306, 350
BstMAI GTCTC 1 cut(s) 289
BstMBI GATC 2 cut(s) 303, 347
BstMWI GCNNNNNNNGC 1 cut(s) 395
BstUI CGCG 2 cut(s) 291, 293
BstV1I GCAGC 2 cut(s) 95, 98
BstV2I GAAGAC 2 cut(s) 43, 387
BstX2I RGATCY 1 cut(s) 303
BstYI RGATCY 1 cut(s) 303
Cac8I GCNNGC 1 cut(s) 122
CfoI GCGC 1 cut(s) 293
CviAII CATG 2 cut(s) 98, 173
CviJI RGCY 4 cut(s) 21, 166, 182, 311
CviKI_1 RGCY 4 cut(s) 21, 166, 182, 311
DdeI CTNAG 2 cut(s) 162, 299
DpnI GATC 2 cut(s) 305, 349
DpnII GATC 2 cut(s) 303, 347
DriI GACNNNNNGTC 1 cut(s) 249
Eam1105I GACNNNNNGTC 1 cut(s) 249
Eco130I CCWWGG 1 cut(s) 177
Eco57I CTGAAG 1 cut(s) 37
EcoT14I CCWWGG 1 cut(s) 177
EcoT22I ATGCAT 1 cut(s) 400
ErhI CCWWGG 1 cut(s) 177
FaeI CATG 2 cut(s) 101, 176
FaiI YATR 9 cut(s) 18, 99, 126, 137, 174, 314, 366, 411, 413
FalI AAGNNNNNCTT 2 cut(s) 35, 67
FatI CATG 2 cut(s) 97, 172
Fnu4HI GCNGC 2 cut(s) 84, 87
Fsp4HI GCNGC 2 cut(s) 84, 87
FspBI CTAG 2 cut(s) 183, 215
GlaI GCGC 1 cut(s) 292
GluI GCNGC 2 cut(s) 84, 87
GsuI CTGGAG 1 cut(s) 328
HhaI GCGC 1 cut(s) 293
Hin1II CATG 2 cut(s) 101, 176
Hin6I GCGC 1 cut(s) 291
HinP1I GCGC 1 cut(s) 291
HinfI GANTC 4 cut(s) 10, 139, 169, 432
Hpy188I TCNGA 2 cut(s) 406, 437
Hpy188III TCNNGA 1 cut(s) 345
HpyAV CCTTC 1 cut(s) 484
HpyCH4III ACNGT 1 cut(s) 247
HpyCH4V TGCA 4 cut(s) 42, 371, 398, 422
HpyF10VI GCNNNNNNNGC 1 cut(s) 395
HpyF3I CTNAG 2 cut(s) 162, 299
Hsp92II CATG 2 cut(s) 101, 176
HspAI GCGC 1 cut(s) 291
Kzo9I GATC 2 cut(s) 303, 347
LpnPI CCDG 5 cut(s) 76, 95, 171, 239, 358
Lsp1109I GCAGC 2 cut(s) 95, 98
LweI GCATC 3 cut(s) 256, 358, 385
MaeI CTAG 2 cut(s) 183, 215
MaeIII GTNAC 1 cut(s) 257
MalI GATC 2 cut(s) 305, 349
MboI GATC 2 cut(s) 303, 347
MboII GAAGA 5 cut(s) 43, 346, 392, 468, 471
MflI RGATCY 1 cut(s) 303
MluCI AATT 2 cut(s) 77, 465
MlyI GAGTC 2 cut(s) 19, 178
MmeI TCCRAC 3 cut(s) 167, 300, 304
MnlI CCTC 1 cut(s) 352
Mph1103I ATGCAT 1 cut(s) 400
MroXI GAANNNNTTC 1 cut(s) 50
MseI TTAA 1 cut(s) 285
MvnI CGCG 2 cut(s) 291, 293
MwoI GCNNNNNNNGC 1 cut(s) 395
NdeII GATC 2 cut(s) 303, 347
NlaIII CATG 2 cut(s) 101, 176
NlaIV GGNNCC 2 cut(s) 156, 305
NmeAIII GCCGAG 1 cut(s) 192
NsiI ATGCAT 1 cut(s) 400
PdmI GAANNNNTTC 1 cut(s) 50
PfeI GAWTC 2 cut(s) 139, 432
PkrI GCNGC 2 cut(s) 85, 88
PleI GAGTC 2 cut(s) 18, 177
PpsI GAGTC 2 cut(s) 18, 177
PshBI ATTAAT 1 cut(s) 285
PspN4I GGNNCC 2 cut(s) 156, 305
PsuI RGATCY 1 cut(s) 303
SaqAI TTAA 1 cut(s) 285
SatI GCNGC 2 cut(s) 84, 87
Sau3AI GATC 2 cut(s) 303, 347
SchI GAGTC 2 cut(s) 19, 178
SetI ASST 3 cut(s) 23, 156, 313
SfaNI GCATC 3 cut(s) 256, 358, 385
SpeI ACTAGT 1 cut(s) 214
Sse9I AATT 2 cut(s) 77, 465
SspMI CTAG 2 cut(s) 183, 215
StyI CCWWGG 1 cut(s) 177
TaaI ACNGT 1 cut(s) 247
TasI AATT 2 cut(s) 77, 465
TfiI GAWTC 2 cut(s) 139, 432
Tru1I TTAA 1 cut(s) 285
Tru9I TTAA 1 cut(s) 285
TseI GCWGC 2 cut(s) 83, 86
TspDTI ATGAA 1 cut(s) 375
VspI ATTAAT 1 cut(s) 285
XapI RAATTY 1 cut(s) 465
XcmI CCANNNNNNNNNTGG 2 cut(s) 76, 325
XmnI GAANNNNTTC 1 cut(s) 50
XspI CTAG 2 cut(s) 183, 215
Zsp2I ATGCAT 1 cut(s) 400
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.