pycom05g13750

aminoacylase activity

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
pyrus_communis
Chr5
OG0004417
Physical Location & Seq
Reverse (-)
17298506 .. 17301819
3314 bp
Loading structure...
UTR
Exon/CDS
Intron
N/A

Sequence Viewer

Length: 1332 bp
ATGGAGCTTCGTCTCCATCTCTGCATAATCTCTCTGCTACTGCTCTCATCAAAATCAACGGCCATACAATCCGACGATGATCCGATAATCTCGCGGTTCCAACGATACCTACGAATCAACACGGCCCACCCAAATCCCCAGTACCGTGAAGCAGCGGATTTCATTCTCTCCGAGGCCAAGTCACTCTCCCTTGAATCCCAGACTCTCGAGTTCGTCCCCGGCAAGCCGCTTGTCCTCCTCAAATGGCCCGGCTCCGACCCCTCCCTCCACTCCGTTCTTCTCAATTCCCACACCGACGTCGTTCCGGCCGAGCACGACAAGTGGGTACACCCCCCTTTCTCGGCCAACTTGGACTCGAACGGCGACGTTTACGCCAGGGGGTCCCAGGACATGAAGTGCGTCGGGATTCAGTACCTGGAGGCGATTCGACGGCTCAAGGCTTCTGGGTTTAAACCCAAAAGGTCTGTTTATTTGTCGTTTGTTCCTGATGAGGAGATTGGGGGACACGACGGTGCTGTGAAGCTGGCTGAATCTGATGTTTTCAAGGGATTGAACGTCGGAATTGTGCTTGATGAAGGGTTGGCGTCGCCAACTGAGACTTACAGAGCATTTTATGCGGAGAGGCGTCCCATGTGGCTGGTGATCGAGGCTACTGGGGCTCCTGGTCATGGGGCTAAGCTCTATGACAACACTGCCATGGAGAATCTCTTGAAAAGCATCGAGAGTGTGAGGCGGTTCCGGGCTGCGCAGTTTGATTTGGTAAAAGTGGGATTGAAGGCTGAAGGCGAGGTCATCTCTGTTAATATTGTGTTTCTGAAAGCTGGCACCCCGACTCCAACTGGTTTTGTAATGAATTTGCAGCCATCTGAAGCGGAAGCAGGCTTTGATATTAGGGTCCCACCAATTGCTGATCATGAGTCTTTGGAGAAACGGATAGCTGAAGAATGGGCCCCTACTTCACGCAACATGACATTTAGGTTGAAGCAGAAGGTTTCGGTGCTTGATAAGTCGGGGAAGCCAATCCTTTCAGCAACTGACAGTTCAAACCCCTGGTGGGCTCTTCTTGAAGATGCTGTCAAAAAAGCTAATGGGAAACTTGGTAAGCCAGAGATTTTTCCCGCTTCAACAGATGCTCACTACTTCCGGAATCTAGGCTTGCCAGCAATCGGATTCTCTCCAATGGCCAACACTCCCATTCTTCTTCATGACCACAATGAGTTTCTGAACAAAGACGAATACTTGAAAGGAATTGCTATTTATGAATCTATTATCAAGGCGTATGCATCTTATGTCGATCATGGGAAAATCGCGGGTTCCCGGGATGAGTTGTAA

Protein Analysis

444

Amino Acids

49.02

Weight (kDa)

6.14

Isoelectric Point (pI)

35.55

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
Peptidase_M20 PF01546 93 - 425 5.6e-35 Peptidase family M20/M25/M40
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AatII GACGTC 1 cut(s) 300
Acc16I TGCGCA 1 cut(s) 747
AccB1I GGYRCC 1 cut(s) 824
AccII CGCG 2 cut(s) 94, 1310
AccIII TCCGGA 1 cut(s) 1143
AciI CCGC 8 cut(s) 94, 155, 227, 617, 733, 872, 1119, 1310
AclWI GGATC 1 cut(s) 74
AcoI YGGCCR 4 cut(s) 60, 306, 342, 1182
AcsI RAATTY 1 cut(s) 853
AcuI CTGAAG 3 cut(s) 801, 888, 960
AcyI GRCGYC 3 cut(s) 297, 584, 625
AfaI GTAC 3 cut(s) 143, 327, 413
AfiI CCNNNNNNNGG 4 cut(s) 340, 668, 1054, 1166
AjnI CCWGG 5 cut(s) 374, 384, 414, 661, 1049
AleI CACNNNNGTG 1 cut(s) 510
AluBI AGCT 6 cut(s) 7, 523, 679, 821, 938, 1085
AluI AGCT 6 cut(s) 7, 523, 679, 821, 938, 1085
Alw21I GWGCWC 1 cut(s) 315
Alw26I GTCTC 2 cut(s) 17, 590
AlwI GGATC 1 cut(s) 74
AlwNI CAGNNNCTG 2 cut(s) 415, 1034
Ama87I CYCGRG 2 cut(s) 206, 1317
Aor13HI TCCGGA 1 cut(s) 1143
AoxI GGCC 8 cut(s) 60, 123, 174, 245, 306, 342, 948, 1182
ApaI GGGCCC 1 cut(s) 952
ApeKI GCWGC 3 cut(s) 152, 743, 859
ApoI RAATTY 1 cut(s) 853
AspLEI GCGC 1 cut(s) 748
AspS9I GGNCC 6 cut(s) 124, 246, 381, 895, 948, 949
AsuC2I CCSGG 5 cut(s) 219, 249, 740, 1318, 1319
AsuHPI GGTGA 1 cut(s) 652
AvaI CYCGRG 2 cut(s) 206, 1317
AvaII GGWCC 2 cut(s) 381, 895
BaeGI GKGCMC 1 cut(s) 952
BalI TGGCCA 1 cut(s) 1184
BanI GGYRCC 1 cut(s) 824
BanII GRGCYC 3 cut(s) 661, 952, 1060
Bbv12I GWGCWC 1 cut(s) 315
BbvI GCAGC 3 cut(s) 164, 730, 871
BccI CCATC 2 cut(s) 24, 871
BceAI ACGGC 4 cut(s) 75, 138, 376, 446
BciT130I CCWGG 5 cut(s) 376, 386, 416, 663, 1051
BclI TGATCA 1 cut(s) 910
BcnI CCSGG 5 cut(s) 219, 249, 740, 1318, 1319
BcoDI GTCTC 2 cut(s) 17, 590
BfaI CTAG 1 cut(s) 1151
BisI GCNGC 4 cut(s) 153, 227, 744, 860
BlpI GCTNAGC 1 cut(s) 675
BlsI GCNGC 4 cut(s) 154, 228, 745, 861
Bme18I GGWCC 2 cut(s) 381, 895
BmeT110I CYCGRG 2 cut(s) 206, 1317
BmgT120I GGNCC 6 cut(s) 124, 246, 381, 895, 948, 949
BmrI ACTGGG 2 cut(s) 133, 663
BmsI GCATC 4 cut(s) 726, 1060, 1120, 1292
BmuI ACTGGG 2 cut(s) 133, 663
BplI GAGNNNNNCTC 2 cut(s) 779, 811
BpmI CTGGAG 1 cut(s) 437
Bpu1102I GCTNAGC 1 cut(s) 675
BpuEI CTTGAG 1 cut(s) 419
BpuMI CCSGG 5 cut(s) 219, 249, 740, 1318, 1319
BsaHI GRCGYC 3 cut(s) 297, 584, 625
BsaJI CCNNGG 7 cut(s) 171, 217, 375, 384, 696, 1049, 1317
BsaWI WCCGGW 1 cut(s) 1143
BsaXI ACNNNNNCTCC 4 cut(s) 643, 673, 817, 847
Bsc4I CCNNNNNNNGG 4 cut(s) 340, 668, 1054, 1166
Bse1I ACTGG 3 cut(s) 139, 658, 844
BseAI TCCGGA 1 cut(s) 1143
BseBI CCWGG 5 cut(s) 376, 386, 416, 663, 1051
BseDI CCNNGG 7 cut(s) 171, 217, 375, 384, 696, 1049, 1317
BseGI GGATG 1 cut(s) 1327
BseLI CCNNNNNNNGG 4 cut(s) 340, 668, 1054, 1166
BseMII CTCAG 1 cut(s) 585
BseNI ACTGG 3 cut(s) 139, 658, 844
BseRI GAGGAG 2 cut(s) 227, 506
BseSI GKGCMC 1 cut(s) 952
BseX3I CGGCCG 1 cut(s) 306
BseXI GCAGC 3 cut(s) 164, 730, 871
Bsh1236I CGCG 2 cut(s) 94, 1310
Bsh1285I CGRYCG 1 cut(s) 309
BshFI GGCC 8 cut(s) 62, 125, 176, 247, 308, 344, 950, 1184
BshNI GGYRCC 1 cut(s) 824
BsiEI CGRYCG 1 cut(s) 309
BsiHKAI GWGCWC 1 cut(s) 315
BsiHKCI CYCGRG 2 cut(s) 206, 1317
BsiSI CCGG 6 cut(s) 219, 249, 305, 739, 1144, 1318
BslFI GGGAC 5 cut(s) 200, 367, 516, 612, 881
BslI CCNNNNNNNGG 4 cut(s) 340, 668, 1054, 1166
BsmAI GTCTC 2 cut(s) 17, 590
BsmBI CGTCTC 1 cut(s) 17
BsmFI GGGAC 5 cut(s) 200, 367, 516, 612, 881
BsnI GGCC 8 cut(s) 62, 125, 176, 247, 308, 344, 950, 1184
BsoBI CYCGRG 2 cut(s) 206, 1317
Bsp120I GGGCCC 1 cut(s) 948
Bsp1286I GDGCHC 4 cut(s) 315, 661, 952, 1060
Bsp13I TCCGGA 1 cut(s) 1143
Bsp143I GATC 4 cut(s) 79, 642, 910, 1294
Bsp1720I GCTNAGC 1 cut(s) 675
Bsp19I CCATGG 1 cut(s) 696
BspACI CCGC 8 cut(s) 94, 155, 227, 617, 733, 872, 1119, 1310
BspANI GGCC 8 cut(s) 62, 125, 176, 247, 308, 344, 950, 1184
BspCNI CTCAG 1 cut(s) 586
BspEI TCCGGA 1 cut(s) 1143
BspFNI CGCG 2 cut(s) 94, 1310
BspHI TCATGA 2 cut(s) 913, 1204
BspPI GGATC 1 cut(s) 74
BspQI GCTCTTC 1 cut(s) 1065
BspT107I GGYRCC 1 cut(s) 824
BsrI ACTGG 3 cut(s) 139, 658, 844
BssECI CCNNGG 7 cut(s) 171, 217, 375, 384, 696, 1049, 1317
BssMI GATC 4 cut(s) 79, 642, 910, 1294
BssNI GRCGYC 3 cut(s) 297, 584, 625
BssT1I CCWWGG 1 cut(s) 696
Bst2UI CCWGG 5 cut(s) 376, 386, 416, 663, 1051
Bst4CI ACNGT 3 cut(s) 146, 512, 1040
Bst6I CTCTTC 1 cut(s) 1065
BstACI GRCGYC 3 cut(s) 297, 584, 625
BstAPI GCANNNNNTGC 1 cut(s) 614
BstC8I GCNNGC 6 cut(s) 224, 525, 823, 880, 1157, 1161
BstDEI CTNAG 2 cut(s) 594, 675
BstDSI CCRYGG 1 cut(s) 696
BstF5I GGATG 1 cut(s) 1327
BstFNI CGCG 2 cut(s) 94, 1310
BstHHI GCGC 1 cut(s) 748
BstKTI GATC 4 cut(s) 82, 645, 913, 1297
BstMAI GTCTC 2 cut(s) 17, 590
BstMBI GATC 4 cut(s) 79, 642, 910, 1294
BstMCI CGRYCG 1 cut(s) 309
BstMWI GCNNNNNNNGC 2 cut(s) 614, 656
BstNI CCWGG 5 cut(s) 376, 386, 416, 663, 1051
BstSLI GKGCMC 1 cut(s) 952
BstUI CGCG 2 cut(s) 94, 1310
BstV1I GCAGC 3 cut(s) 164, 730, 871
BstXI CCANNNNNNTGG 1 cut(s) 637
BstZI CGGCCG 1 cut(s) 306
BsuRI GGCC 8 cut(s) 62, 125, 176, 247, 308, 344, 950, 1184
BtgI CCRYGG 1 cut(s) 696
BtsCI GGATG 1 cut(s) 1327
BtsI GCAGTG 1 cut(s) 690
BtsIMutI CAGTG 1 cut(s) 690
Cac8I GCNNGC 6 cut(s) 224, 525, 823, 880, 1157, 1161
CaiI CAGNNNCTG 2 cut(s) 415, 1034
CciI TCATGA 2 cut(s) 913, 1204
CfoI GCGC 1 cut(s) 748
Cfr13I GGNCC 6 cut(s) 124, 246, 381, 895, 948, 949
Cfr9I CCCGGG 1 cut(s) 1317
CseI GACGC 3 cut(s) 388, 573, 614
Csp6I GTAC 3 cut(s) 142, 326, 412
CviAII CATG 8 cut(s) 391, 631, 668, 697, 914, 967, 1205, 1298
CviQI GTAC 3 cut(s) 142, 326, 412
DdeI CTNAG 2 cut(s) 594, 675
DpnI GATC 4 cut(s) 81, 644, 912, 1296
DpnII GATC 4 cut(s) 79, 642, 910, 1294
DraI TTTAAA 1 cut(s) 451
EaeI YGGCCR 4 cut(s) 60, 306, 342, 1182
EagI CGGCCG 1 cut(s) 306
Eam1104I CTCTTC 1 cut(s) 1065
EarI CTCTTC 1 cut(s) 1065
EclXI CGGCCG 1 cut(s) 306
Eco130I CCWWGG 1 cut(s) 696
Eco24I GRGCYC 3 cut(s) 661, 952, 1060
Eco47I GGWCC 2 cut(s) 381, 895
Eco52I CGGCCG 1 cut(s) 306
Eco57I CTGAAG 3 cut(s) 801, 888, 960
Eco88I CYCGRG 2 cut(s) 206, 1317
EcoO109I RGGNCCY 3 cut(s) 381, 895, 949
EcoRII CCWGG 5 cut(s) 374, 384, 414, 661, 1049
EcoT14I CCWWGG 1 cut(s) 696
EcoT22I ATGCAT 1 cut(s) 1285
EcoT38I GRGCYC 3 cut(s) 661, 952, 1060
ErhI CCWWGG 1 cut(s) 696
Esp3I CGTCTC 1 cut(s) 17
FaeI CATG 8 cut(s) 394, 634, 671, 700, 917, 970, 1208, 1301
FaqI GGGAC 5 cut(s) 200, 367, 516, 612, 881
FatI CATG 8 cut(s) 390, 630, 667, 696, 913, 966, 1204, 1297
FauI CCCGC 2 cut(s) 1126, 1303
FbaI TGATCA 1 cut(s) 910
Fnu4HI GCNGC 4 cut(s) 153, 227, 744, 860
FriOI GRGCYC 3 cut(s) 661, 952, 1060
Fsp4HI GCNGC 4 cut(s) 153, 227, 744, 860
FspBI CTAG 1 cut(s) 1151
FspI TGCGCA 1 cut(s) 747
GlaI GCGC 1 cut(s) 747
GluI GCNGC 4 cut(s) 153, 227, 744, 860
GsuI CTGGAG 1 cut(s) 437
HaeIII GGCC 8 cut(s) 62, 125, 176, 247, 308, 344, 950, 1184
HapII CCGG 6 cut(s) 219, 249, 305, 739, 1144, 1318
HgaI GACGC 3 cut(s) 388, 573, 614
HhaI GCGC 1 cut(s) 748
Hin1I GRCGYC 3 cut(s) 297, 584, 625
Hin1II CATG 8 cut(s) 394, 634, 671, 700, 917, 970, 1208, 1301
Hin6I GCGC 1 cut(s) 746
HinP1I GCGC 1 cut(s) 746
HpaII CCGG 6 cut(s) 219, 249, 305, 739, 1144, 1318
HphI GGTGA 1 cut(s) 652
Hpy166II GTNNAC 2 cut(s) 328, 370
Hpy188III TCNNGA 9 cut(s) 206, 403, 485, 709, 721, 914, 1064, 1144, 1205
Hpy8I GTNNAC 2 cut(s) 328, 370
Hpy99I CGWCG 9 cut(s) 77, 299, 302, 368, 404, 432, 512, 560, 589
HpyAV CCTTC 4 cut(s) 569, 769, 776, 982
HpyCH4III ACNGT 3 cut(s) 146, 512, 1040
HpyCH4IV ACGT 3 cut(s) 297, 366, 555
HpyCH4V TGCA 3 cut(s) 24, 859, 1283
HpyF10VI GCNNNNNNNGC 2 cut(s) 614, 656
HpyF3I CTNAG 2 cut(s) 594, 675
HpySE526I ACGT 3 cut(s) 297, 366, 555
Hsp92I GRCGYC 3 cut(s) 297, 584, 625
Hsp92II CATG 8 cut(s) 394, 634, 671, 700, 917, 970, 1208, 1301
HspAI GCGC 1 cut(s) 746
KflI GGGWCCC 2 cut(s) 381, 895
Kpn2I TCCGGA 1 cut(s) 1143
Ksp22I TGATCA 1 cut(s) 910
Kzo9I GATC 4 cut(s) 79, 642, 910, 1294
LguI GCTCTTC 1 cut(s) 1065
LmnI GCTCC 3 cut(s) 4, 257, 664
Lsp1109I GCAGC 3 cut(s) 164, 730, 871
LweI GCATC 4 cut(s) 726, 1060, 1120, 1292
MaeI CTAG 1 cut(s) 1151
MaeII ACGT 3 cut(s) 297, 366, 555
MaeIII GTNAC 1 cut(s) 180
MalI GATC 4 cut(s) 81, 644, 912, 1296
MboI GATC 4 cut(s) 79, 642, 910, 1294
MboII GAAGA 6 cut(s) 269, 953, 1052, 1079, 1190, 1193
MfeI CAATTG 1 cut(s) 903
MhlI GDGCHC 4 cut(s) 315, 661, 952, 1060
MlsI TGGCCA 1 cut(s) 1184
MluCI AATT 5 cut(s) 283, 561, 853, 903, 1248
MluNI TGGCCA 1 cut(s) 1184
MlyI GAGTC 4 cut(s) 196, 347, 826, 926
MmeI TCCRAC 5 cut(s) 96, 124, 279, 538, 860
Mox20I TGGCCA 1 cut(s) 1184
Mph1103I ATGCAT 1 cut(s) 1285
MroI TCCGGA 1 cut(s) 1143
MscI TGGCCA 1 cut(s) 1184
MseI TTAA 2 cut(s) 450, 801
MslI CAYNNNNRTG 2 cut(s) 510, 695
Msp20I TGGCCA 1 cut(s) 1184
MspA1I CMGCKG 1 cut(s) 155
MspI CCGG 6 cut(s) 219, 249, 305, 739, 1144, 1318
MssI GTTTAAAC 1 cut(s) 451
MunI CAATTG 1 cut(s) 903
MvaI CCWGG 5 cut(s) 376, 386, 416, 663, 1051
MvnI CGCG 2 cut(s) 94, 1310
MwoI GCNNNNNNNGC 2 cut(s) 614, 656
NciI CCSGG 5 cut(s) 219, 249, 740, 1318, 1319
NcoI CCATGG 1 cut(s) 696
NdeII GATC 4 cut(s) 79, 642, 910, 1294
NlaIII CATG 8 cut(s) 394, 634, 671, 700, 917, 970, 1208, 1301
NmeAIII GCCGAG 2 cut(s) 320, 334
NmuCI GTSAC 1 cut(s) 180
NsbI TGCGCA 1 cut(s) 747
NsiI ATGCAT 1 cut(s) 1285
OliI CACNNNNGTG 1 cut(s) 510
PaeR7I CTCGAG 1 cut(s) 206
PagI TCATGA 2 cut(s) 913, 1204
PciSI GCTCTTC 1 cut(s) 1065
PcsI WCGNNNNNNNCGW 2 cut(s) 109, 306
PfeI GAWTC 9 cut(s) 114, 194, 406, 424, 530, 703, 1147, 1170, 1262
PkrI GCNGC 4 cut(s) 154, 228, 745, 861
PleI GAGTC 4 cut(s) 196, 347, 826, 925
PmeI GTTTAAAC 1 cut(s) 451
PpsI GAGTC 4 cut(s) 196, 347, 826, 925
PpuMI RGGWCCY 2 cut(s) 381, 895
Psp5II RGGWCCY 2 cut(s) 381, 895
Psp6I CCWGG 5 cut(s) 374, 384, 414, 661, 1049
PspGI CCWGG 5 cut(s) 374, 384, 414, 661, 1049
PspOMI GGGCCC 1 cut(s) 948
PspPI GGNCC 6 cut(s) 124, 246, 381, 895, 948, 949
PspPPI RGGWCCY 2 cut(s) 381, 895
PstNI CAGNNNCTG 2 cut(s) 415, 1034
RsaI GTAC 3 cut(s) 143, 327, 413
RsaNI GTAC 3 cut(s) 142, 326, 412
RseI CAYNNNNRTG 2 cut(s) 510, 695
SapI GCTCTTC 1 cut(s) 1065
SaqAI TTAA 2 cut(s) 450, 801
SatI GCNGC 4 cut(s) 153, 227, 744, 860
Sau3AI GATC 4 cut(s) 79, 642, 910, 1294
Sau96I GGNCC 6 cut(s) 124, 246, 381, 895, 948, 949
SchI GAGTC 4 cut(s) 196, 347, 826, 926
SduI GDGCHC 4 cut(s) 315, 661, 952, 1060
SfaNI GCATC 4 cut(s) 726, 1060, 1120, 1292
Sfr274I CTCGAG 1 cut(s) 206
SinI GGWCC 2 cut(s) 381, 895
SlaI CTCGAG 1 cut(s) 206
SmaI CCCGGG 1 cut(s) 1319
SmiMI CAYNNNNRTG 2 cut(s) 510, 695
SmlI CTYRAG 2 cut(s) 206, 434
SmoI CTYRAG 2 cut(s) 206, 434
Sse9I AATT 5 cut(s) 283, 561, 853, 903, 1248
SsiI CCGC 8 cut(s) 94, 155, 227, 617, 733, 872, 1119, 1310
SspI AATATT 1 cut(s) 805
SspMI CTAG 1 cut(s) 1151
StyI CCWWGG 1 cut(s) 696
TaaI ACNGT 3 cut(s) 146, 512, 1040
TaiI ACGT 3 cut(s) 300, 369, 558
TaqI TCGA 6 cut(s) 207, 356, 427, 645, 720, 1293
TasI AATT 5 cut(s) 283, 561, 853, 903, 1248
TauI GCSGC 1 cut(s) 229
TfiI GAWTC 9 cut(s) 114, 194, 406, 424, 530, 703, 1147, 1170, 1262
Tru1I TTAA 2 cut(s) 450, 801
Tru9I TTAA 2 cut(s) 450, 801
TscAI CASTG 1 cut(s) 697
TseFI GTSAC 1 cut(s) 180
TseI GCWGC 3 cut(s) 152, 743, 859
Tsp45I GTSAC 1 cut(s) 180
TspDTI ATGAA 6 cut(s) 151, 407, 588, 866, 1193, 1275
TspGWI ACGGA 2 cut(s) 262, 946
TspMI CCCGGG 1 cut(s) 1317
TspRI CASTG 1 cut(s) 697
VpaK11BI GGWCC 2 cut(s) 381, 895
XapI RAATTY 1 cut(s) 853
XhoI CTCGAG 1 cut(s) 206
XmaI CCCGGG 1 cut(s) 1317
XspI CTAG 1 cut(s) 1151
ZraI GACGTC 1 cut(s) 298
Zsp2I ATGCAT 1 cut(s) 1285
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.