pycom05g16460

No description available

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
pyrus_communis
Chr5
OG0007020
Physical Location & Seq
Reverse (-)
19724686 .. 19726085
1400 bp
Loading structure...
UTR
Exon/CDS
Intron
pycom05g16460.4

Sequence Viewer

Length: 666 bp
ATGCAGCTTCTTCAATGGCTATTTGGGGCTAAAGTTGGACAAGGAAGGGAGGAGATTTCTAGTTCTTCCAACAAGAACGCAACAACTAATGATCAAGAAGCTAAACCAAATTACATAATCGTCTTCAGGCGTGGATGCGTCGGTGTTTATCGAAAATCATCAAAAGGAGTTGAAGAAAGCAAATCAGGTAGCAAGAAGTTCAAGCCGTTTACCTTTCTATGTCGAAAGGTTGTTGCAACACGTTGCTTTAATAGCACTCTTCAGCTAAAGAGACTTAATAGCATAAACAGAAGGCAGGCAAGGCAGCATTGGATTCAATCTATGAAGATCATGAAGAAAGAAGATATTGCAAAAGGTCTGGGAATCAGTAGCACCAAGGCGGATTCGGTGAATGTAAGCAACAAGGTTTTACCGGTGAATGATTCAACGTTATCAACCTTGACGAGCACAAACGAACAATGTGGCTTACCGGAGAAGAAAGAACAGTTGAAGGGGGACAAAACCAAAACCCTGTCAAGAATGAAGGAGCTTCTGAGATGGGCTGCTGCTGCAAAATCAGACAAGGGTGGAAAATTCATCGCTCGAAAGGTTAAGCAATTTCGAAACCGGGGAACTCTGAAAGCGGTACCAGATGACGATCAACTAAGCAACGACTCGCCCCTTTGA
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
No pathways identified.
Pfam Domains
Protein Families
No domains found.

Protein Analysis

222

Amino Acids

24.76

Weight (kDa)

10.2

Isoelectric Point (pI)

39.66

Instability Index
Protein Domains (Pfam)
No Pfam domains detected for this protein.
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Publication-ready
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Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
Acc65I GGTACC 1 cut(s) 625
AccB1I GGYRCC 1 cut(s) 625
AciI CCGC 2 cut(s) 380, 623
AclI AACGTT 1 cut(s) 428
AcsI RAATTY 1 cut(s) 572
AcuI CTGAAG 2 cut(s) 109, 245
AfaI GTAC 1 cut(s) 627
AflIII ACRYGT 1 cut(s) 239
AgeI ACCGGT 1 cut(s) 412
AgsI TTSAA 6 cut(s) 14, 173, 202, 317, 426, 490
AluBI AGCT 4 cut(s) 7, 101, 265, 529
AluI AGCT 4 cut(s) 7, 101, 265, 529
Alw21I GWGCWC 1 cut(s) 449
Alw26I GTCTC 1 cut(s) 265
ApeKI GCWGC 5 cut(s) 4, 304, 542, 545, 548
ApoI RAATTY 1 cut(s) 572
AsiGI ACCGGT 1 cut(s) 412
Asp718I GGTACC 1 cut(s) 625
AsuC2I CCSGG 1 cut(s) 608
AsuHPI GGTGA 2 cut(s) 400, 427
AsuII TTCGAA 1 cut(s) 601
BanI GGYRCC 1 cut(s) 625
BbsI GAAGAC 1 cut(s) 115
Bbv12I GWGCWC 1 cut(s) 449
BbvI GCAGC 5 cut(s) 16, 316, 529, 532, 535
BccI CCATC 1 cut(s) 531
BceAI ACGGC 1 cut(s) 190
BclI TGATCA 1 cut(s) 91
BcnI CCSGG 1 cut(s) 608
BcoDI GTCTC 1 cut(s) 265
BfaI CTAG 1 cut(s) 60
BisI GCNGC 5 cut(s) 5, 305, 543, 546, 549
BlsI GCNGC 5 cut(s) 6, 306, 544, 547, 550
Bme1390I CCNGG 1 cut(s) 608
BmiI GGNNCC 1 cut(s) 627
BmrFI CCNGG 1 cut(s) 608
BmsI GCATC 1 cut(s) 125
BpiI GAAGAC 1 cut(s) 115
Bpu14I TTCGAA 1 cut(s) 601
BpuMI CCSGG 1 cut(s) 608
BsaBI GATNNNNATC 1 cut(s) 636
BsaJI CCNNGG 2 cut(s) 375, 607
BsaWI WCCGGW 2 cut(s) 412, 469
Bse118I RCCGGY 1 cut(s) 412
Bse8I GATNNNNATC 1 cut(s) 636
BseDI CCNNGG 2 cut(s) 375, 607
BseGI GGATG 1 cut(s) 140
BseJI GATNNNNATC 1 cut(s) 636
BseMII CTCAG 1 cut(s) 524
BseRI GAGGAG 1 cut(s) 65
BseXI GCAGC 5 cut(s) 16, 316, 529, 532, 535
BshNI GGYRCC 1 cut(s) 625
BshTI ACCGGT 1 cut(s) 412
BsiHKAI GWGCWC 1 cut(s) 449
BsiSI CCGG 3 cut(s) 413, 470, 607
BslFI GGGAC 1 cut(s) 509
BsmAI GTCTC 1 cut(s) 265
BsmFI GGGAC 1 cut(s) 509
Bsp119I TTCGAA 1 cut(s) 601
Bsp1286I GDGCHC 1 cut(s) 449
Bsp143I GATC 3 cut(s) 91, 327, 637
BspACI CCGC 2 cut(s) 380, 623
BspCNI CTCAG 1 cut(s) 525
BspHI TCATGA 1 cut(s) 330
BspLI GGNNCC 1 cut(s) 627
BspT104I TTCGAA 1 cut(s) 601
BspT107I GGYRCC 1 cut(s) 625
BsrFI RCCGGY 1 cut(s) 412
BssAI RCCGGY 1 cut(s) 412
BssECI CCNNGG 2 cut(s) 375, 607
BssMI GATC 3 cut(s) 91, 327, 637
BssT1I CCWWGG 1 cut(s) 375
Bst4CI ACNGT 1 cut(s) 486
Bst6I CTCTTC 1 cut(s) 264
BstBI TTCGAA 1 cut(s) 601
BstC8I GCNNGC 1 cut(s) 297
BstDEI CTNAG 2 cut(s) 533, 644
BstF5I GGATG 1 cut(s) 140
BstKTI GATC 3 cut(s) 94, 330, 640
BstMAI GTCTC 1 cut(s) 265
BstMBI GATC 3 cut(s) 91, 327, 637
BstMWI GCNNNNNNNGC 3 cut(s) 252, 301, 548
BstSCI CCNGG 1 cut(s) 606
BstV1I GCAGC 5 cut(s) 16, 316, 529, 532, 535
BstV2I GAAGAC 1 cut(s) 115
BtgZI GCGATG 1 cut(s) 562
BtsCI GGATG 1 cut(s) 140
Cac8I GCNNGC 1 cut(s) 297
CciI TCATGA 1 cut(s) 330
Cfr10I RCCGGY 1 cut(s) 412
CseI GACGC 1 cut(s) 127
Csp6I GTAC 1 cut(s) 626
CspAI ACCGGT 1 cut(s) 412
CviAII CATG 1 cut(s) 331
CviJI RGCY 9 cut(s) 7, 19, 29, 101, 205, 265, 465, 529, 542
CviKI_1 RGCY 9 cut(s) 7, 19, 29, 101, 205, 265, 465, 529, 542
CviQI GTAC 1 cut(s) 626
DdeI CTNAG 2 cut(s) 533, 644
DpnI GATC 3 cut(s) 93, 329, 639
DpnII GATC 3 cut(s) 91, 327, 637
Eam1104I CTCTTC 1 cut(s) 264
EarI CTCTTC 1 cut(s) 264
EciI GGCGGA 1 cut(s) 395
Eco130I CCWWGG 1 cut(s) 375
Eco57I CTGAAG 2 cut(s) 109, 245
EcoT14I CCWWGG 1 cut(s) 375
ErhI CCWWGG 1 cut(s) 375
FaeI CATG 1 cut(s) 334
FaiI YATR 5 cut(s) 116, 220, 284, 323, 332
FaqI GGGAC 1 cut(s) 509
FatI CATG 1 cut(s) 330
FbaI TGATCA 1 cut(s) 91
Fnu4HI GCNGC 5 cut(s) 5, 305, 543, 546, 549
FokI GGATG 1 cut(s) 147
Fsp4HI GCNGC 5 cut(s) 5, 305, 543, 546, 549
FspBI CTAG 1 cut(s) 60
GluI GCNGC 5 cut(s) 5, 305, 543, 546, 549
HapII CCGG 3 cut(s) 413, 470, 607
HgaI GACGC 1 cut(s) 127
Hin1II CATG 1 cut(s) 334
HinfI GANTC 5 cut(s) 313, 363, 383, 422, 653
HpaII CCGG 3 cut(s) 413, 470, 607
HphI GGTGA 2 cut(s) 400, 427
Hpy166II GTNNAC 1 cut(s) 210
Hpy188I TCNGA 3 cut(s) 534, 559, 618
Hpy188III TCNNGA 3 cut(s) 95, 331, 516
Hpy8I GTNNAC 1 cut(s) 210
Hpy99I CGWCG 1 cut(s) 143
HpyAV CCTTC 4 cut(s) 39, 285, 484, 517
HpyCH4III ACNGT 1 cut(s) 486
HpyCH4IV ACGT 2 cut(s) 241, 428
HpyCH4V TGCA 4 cut(s) 4, 236, 350, 551
HpyF10VI GCNNNNNNNGC 3 cut(s) 252, 301, 548
HpyF3I CTNAG 2 cut(s) 533, 644
HpySE526I ACGT 2 cut(s) 241, 428
Hsp92II CATG 1 cut(s) 334
KpnI GGTACC 1 cut(s) 629
Ksp22I TGATCA 1 cut(s) 91
Kzo9I GATC 3 cut(s) 91, 327, 637
LmnI GCTCC 1 cut(s) 526
LpnPI CCDG 9 cut(s) 112, 171, 281, 344, 426, 483, 524, 620, 642
Lsp1109I GCAGC 5 cut(s) 16, 316, 529, 532, 535
LweI GCATC 1 cut(s) 125
MaeI CTAG 1 cut(s) 60
MaeII ACGT 2 cut(s) 241, 428
MalI GATC 3 cut(s) 93, 329, 639
MboI GATC 3 cut(s) 91, 327, 637
MboII GAAGA 8 cut(s) 57, 115, 185, 251, 337, 346, 353, 487
MhlI GDGCHC 1 cut(s) 449
MluCI AATT 3 cut(s) 109, 572, 596
MlyI GAGTC 1 cut(s) 647
MmeI TCCRAC 2 cut(s) 16, 93
MnlI CCTC 1 cut(s) 43
MseI TTAA 3 cut(s) 249, 276, 591
MspI CCGG 3 cut(s) 413, 470, 607
MspR9I CCNGG 1 cut(s) 608
MwoI GCNNNNNNNGC 3 cut(s) 252, 301, 548
NciI CCSGG 1 cut(s) 608
NdeII GATC 3 cut(s) 91, 327, 637
NlaIII CATG 1 cut(s) 334
NlaIV GGNNCC 1 cut(s) 627
NspV TTCGAA 1 cut(s) 601
PagI TCATGA 1 cut(s) 330
PfeI GAWTC 4 cut(s) 313, 363, 383, 422
PinAI ACCGGT 1 cut(s) 412
PkrI GCNGC 5 cut(s) 6, 306, 544, 547, 550
PleI GAGTC 1 cut(s) 647
PpsI GAGTC 1 cut(s) 647
Psp1406I AACGTT 1 cut(s) 428
PspN4I GGNNCC 1 cut(s) 627
RsaI GTAC 1 cut(s) 627
RsaNI GTAC 1 cut(s) 626
SaqAI TTAA 3 cut(s) 249, 276, 591
SatI GCNGC 5 cut(s) 5, 305, 543, 546, 549
Sau3AI GATC 3 cut(s) 91, 327, 637
SchI GAGTC 1 cut(s) 647
ScrFI CCNGG 1 cut(s) 608
SduI GDGCHC 1 cut(s) 449
SfaNI GCATC 1 cut(s) 125
SfuI TTCGAA 1 cut(s) 601
Sse9I AATT 3 cut(s) 109, 572, 596
SsiI CCGC 2 cut(s) 380, 623
SspMI CTAG 1 cut(s) 60
StyD4I CCNGG 1 cut(s) 606
StyI CCWWGG 1 cut(s) 375
TaaI ACNGT 1 cut(s) 486
TaiI ACGT 2 cut(s) 244, 431
TaqI TCGA 4 cut(s) 151, 223, 583, 601
TasI AATT 3 cut(s) 109, 572, 596
TfiI GAWTC 4 cut(s) 313, 363, 383, 422
Tru1I TTAA 3 cut(s) 249, 276, 591
Tru9I TTAA 3 cut(s) 249, 276, 591
TseI GCWGC 5 cut(s) 4, 304, 542, 545, 548
TspDTI ATGAA 4 cut(s) 338, 347, 536, 565
XapI RAATTY 1 cut(s) 572
XspI CTAG 1 cut(s) 60
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.