pycom05g20430

Elongation factor

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
pyrus_communis
Chr5
OG0001995
Physical Location & Seq
Reverse (-)
23191436 .. 23192588
1153 bp
Loading structure...
UTR
Exon/CDS
Intron
N/A

Sequence Viewer

Length: 867 bp
ATGGTGAAATTCACAGCTGAGGAGCTCCGTAGGATTATGGACTACAAACACAACATTCGTAATATGTCTGTTATTGCGCACGTCGACCATGGGAAGTCAACTCTTACCGACTCCCTTGTCGCTGCTGCTGGCATCATTGCACAGGAAGTTGCCGGTGATGTCCGTATGACGGATACCCGTGCAGATGAAGCTGAGCGTGGTATCACAATCAAATCTACCGGTATCTCTTTGTACTATGAGATGACTGATGAGTCTTTGCAGAGGTTCAAGGGAGAGAGAAATGGAAATGAGTACCTCATCAATCTCATTGATTCCCCCGGGCACGTTGACTTTTCATCTGAGGTCACAGCTGCTCTGCGTATTACTGATGGTGCACTTGTGGTGGTGGATTGTATTGAGGGTGTGTGTGTCCAGACTGAGACTGTGCTCCGTCAAGCCTTGGGAGAGAGGATCGGTCCTGTTTTGACTGTTAACAAGATGGACAGGTGCTTCCTTGAGCTCCAGGTCGATGGAGAAGAGGCTTACCAGACATTCCAAAGAGTTATTGAAAATGCCAATGTCATTATGGCTACATATGAAGATCCACTTCTTGGTGATGTCCAGGTGTACCCAGAGAAAGGAACCGTTGCCTTTTCAGCTGGTTTGCACGGTTGGGCTTTCACTCTGACCAACTTCGCTAAGATGTATGCTTCCAAGTTTGGAGTTGATGAGTCAAAGATGATGGAAAGGCTCTGGGGTGAGAACTTCTTTGACCCAGCTACCAAGAAATGGACCAGCAAGAACACTGGAACAGCTACTTGCAAGCGTGGTTTTGTTCAGTTCTGTTATGAGCCCATCAAGCAGATCGATCAACACCTGCATGAATGA
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
GO:0005575 GO:0005622 GO:0005623 GO:0005737 GO:0044424 GO:0044464
KEGG Pathways
Metabolic & Signaling
ko04152 ko04921 map04152 map04921
Pfam Domains
Protein Families
EFG_C EFG_II EFG_IV GTP_EFTU GTP_EFTU_D2

Protein Analysis

289

Amino Acids

32.29

Weight (kDa)

5.13

Isoelectric Point (pI)

30.48

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
GTP_EFTU PF00009 18 - 221 5.7e-49 Elongation factor Tu GTP binding domain
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Publication-ready
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Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AasI GACNNNNNNGTC 2 cut(s) 116, 250
Acc16I TGCGCA 1 cut(s) 78
AccB7I CCANNNNNTGG 2 cut(s) 590, 768
AccI GTMKAC 1 cut(s) 84
AclWI GGATC 2 cut(s) 458, 575
AcsI RAATTY 1 cut(s) 8
AfaI GTAC 3 cut(s) 233, 293, 608
AfiI CCNNNNNNNGG 4 cut(s) 169, 590, 617, 768
AgeI ACCGGT 1 cut(s) 218
AgsI TTSAA 2 cut(s) 268, 548
AjiI CACGTC 1 cut(s) 82
AjnI CCWGG 2 cut(s) 501, 600
AluBI AGCT 8 cut(s) 17, 25, 191, 350, 499, 638, 758, 794
AluI AGCT 8 cut(s) 17, 25, 191, 350, 499, 638, 758, 794
Alw21I GWGCWC 4 cut(s) 27, 376, 429, 501
Alw26I GTCTC 1 cut(s) 413
Alw44I GTGCAC 1 cut(s) 372
AlwI GGATC 2 cut(s) 458, 575
Ama87I CYCGRG 1 cut(s) 317
ApaLI GTGCAC 1 cut(s) 372
ApeKI GCWGC 3 cut(s) 122, 125, 350
ApoI RAATTY 1 cut(s) 8
AsiGI ACCGGT 1 cut(s) 218
AspLEI GCGC 1 cut(s) 79
AspS9I GGNCC 2 cut(s) 455, 771
AsuC2I CCSGG 2 cut(s) 318, 319
AsuHPI GGTGA 4 cut(s) 16, 167, 605, 749
AvaI CYCGRG 1 cut(s) 317
AvaII GGWCC 2 cut(s) 455, 771
BaeGI GKGCMC 2 cut(s) 324, 376
BanII GRGCYC 3 cut(s) 27, 501, 834
Bbv12I GWGCWC 4 cut(s) 27, 376, 429, 501
BbvCI CCTCAGC 1 cut(s) 18
BbvI GCAGC 3 cut(s) 109, 112, 337
BccI CCATC 5 cut(s) 362, 472, 503, 715, 842
BciT130I CCWGG 2 cut(s) 503, 602
BciVI GTATCC 1 cut(s) 166
BcnI CCSGG 2 cut(s) 318, 319
BcoDI GTCTC 1 cut(s) 413
BfuI GTATCC 1 cut(s) 166
BisI GCNGC 3 cut(s) 123, 126, 351
BlpI GCTNAGC 1 cut(s) 192
BlsI GCNGC 3 cut(s) 124, 127, 352
Bme1390I CCNGG 4 cut(s) 318, 319, 503, 602
Bme18I GGWCC 2 cut(s) 455, 771
BmeT110I CYCGRG 1 cut(s) 317
BmgBI CACGTC 1 cut(s) 82
BmgT120I GGNCC 2 cut(s) 455, 771
BmiI GGNNCC 1 cut(s) 622
BmrFI CCNGG 4 cut(s) 318, 319, 503, 602
BmsI GCATC 1 cut(s) 141
BpmI CTGGAG 1 cut(s) 485
Bpu10I CCTNAGC 1 cut(s) 18
Bpu1102I GCTNAGC 1 cut(s) 192
BpuEI CTTGAG 1 cut(s) 515
BpuMI CCSGG 2 cut(s) 318, 319
Bsa29I ATCGAT 1 cut(s) 846
BsaJI CCNNGG 4 cut(s) 88, 316, 317, 438
BsaWI WCCGGW 1 cut(s) 218
Bsc4I CCNNNNNNNGG 4 cut(s) 169, 590, 617, 768
Bse118I RCCGGY 2 cut(s) 152, 218
Bse1I ACTGG 1 cut(s) 790
Bse3DI GCAATG 1 cut(s) 135
BseBI CCWGG 2 cut(s) 503, 602
BseCI ATCGAT 1 cut(s) 846
BseDI CCNNGG 4 cut(s) 88, 316, 317, 438
BseLI CCNNNNNNNGG 4 cut(s) 169, 590, 617, 768
BseMI GCAATG 1 cut(s) 135
BseMII CTCAG 4 cut(s) 9, 183, 330, 408
BseNI ACTGG 1 cut(s) 790
BseRI GAGGAG 1 cut(s) 35
BseSI GKGCMC 2 cut(s) 324, 376
BseXI GCAGC 3 cut(s) 109, 112, 337
BseYI CCCAGC 1 cut(s) 754
BsgI GTGCAG 1 cut(s) 201
BshTI ACCGGT 1 cut(s) 218
BshVI ATCGAT 1 cut(s) 846
BsiHKAI GWGCWC 4 cut(s) 27, 376, 429, 501
BsiHKCI CYCGRG 1 cut(s) 317
BsiSI CCGG 3 cut(s) 153, 219, 318
BslI CCNNNNNNNGG 4 cut(s) 169, 590, 617, 768
BsmAI GTCTC 1 cut(s) 413
BsoBI CYCGRG 1 cut(s) 317
Bsp1286I GDGCHC 6 cut(s) 27, 324, 376, 429, 501, 834
Bsp143I GATC 4 cut(s) 450, 580, 843, 847
Bsp1720I GCTNAGC 1 cut(s) 192
Bsp19I CCATGG 1 cut(s) 88
BspCNI CTCAG 4 cut(s) 10, 184, 331, 409
BspDI ATCGAT 1 cut(s) 846
BspLI GGNNCC 1 cut(s) 622
BspPI GGATC 2 cut(s) 458, 575
BsrDI GCAATG 1 cut(s) 135
BsrFI RCCGGY 2 cut(s) 152, 218
BsrI ACTGG 1 cut(s) 790
BssAI RCCGGY 2 cut(s) 152, 218
BssECI CCNNGG 4 cut(s) 88, 316, 317, 438
BssMI GATC 4 cut(s) 450, 580, 843, 847
BssT1I CCWWGG 2 cut(s) 88, 438
Bst2UI CCWGG 2 cut(s) 503, 602
Bst4CI ACNGT 4 cut(s) 424, 469, 625, 650
Bst6I CTCTTC 1 cut(s) 510
BstC8I GCNNGC 2 cut(s) 130, 803
BstDEI CTNAG 5 cut(s) 18, 192, 339, 417, 678
BstDSI CCRYGG 1 cut(s) 88
BstHHI GCGC 1 cut(s) 79
BstKTI GATC 4 cut(s) 453, 583, 846, 850
BstMAI GTCTC 1 cut(s) 413
BstMBI GATC 4 cut(s) 450, 580, 843, 847
BstMWI GCNNNNNNNGC 3 cut(s) 188, 635, 838
BstNI CCWGG 2 cut(s) 503, 602
BstSCI CCNGG 4 cut(s) 316, 317, 501, 600
BstSLI GKGCMC 2 cut(s) 324, 376
BstV1I GCAGC 3 cut(s) 109, 112, 337
BstX2I RGATCY 1 cut(s) 580
BstXI CCANNNNNNTGG 1 cut(s) 509
BstYI RGATCY 1 cut(s) 580
Bsu15I ATCGAT 1 cut(s) 846
BsuI GTATCC 1 cut(s) 166
BsuTUI ATCGAT 1 cut(s) 846
BtgI CCRYGG 1 cut(s) 88
BtrI CACGTC 1 cut(s) 82
BtsIMutI CAGTG 1 cut(s) 783
Cac8I GCNNGC 2 cut(s) 130, 803
CfoI GCGC 1 cut(s) 79
Cfr10I RCCGGY 2 cut(s) 152, 218
Cfr13I GGNCC 2 cut(s) 455, 771
Cfr9I CCCGGG 1 cut(s) 317
ClaI ATCGAT 1 cut(s) 846
Csp6I GTAC 3 cut(s) 232, 292, 607
CspAI ACCGGT 1 cut(s) 218
CviAII CATG 2 cut(s) 89, 860
CviQI GTAC 3 cut(s) 232, 292, 607
DdeI CTNAG 5 cut(s) 18, 192, 339, 417, 678
DpnI GATC 4 cut(s) 452, 582, 845, 849
DpnII GATC 4 cut(s) 450, 580, 843, 847
DrdI GACNNNNNNGTC 2 cut(s) 116, 250
DseDI GACNNNNNNGTC 2 cut(s) 116, 250
Eam1104I CTCTTC 1 cut(s) 510
EarI CTCTTC 1 cut(s) 510
Ecl136II GAGCTC 2 cut(s) 25, 499
Eco130I CCWWGG 2 cut(s) 88, 438
Eco24I GRGCYC 3 cut(s) 27, 501, 834
Eco47I GGWCC 2 cut(s) 455, 771
Eco53kI GAGCTC 2 cut(s) 25, 499
Eco88I CYCGRG 1 cut(s) 317
EcoICRI GAGCTC 2 cut(s) 25, 499
EcoRII CCWGG 2 cut(s) 501, 600
EcoT14I CCWWGG 2 cut(s) 88, 438
EcoT38I GRGCYC 3 cut(s) 27, 501, 834
ErhI CCWWGG 2 cut(s) 88, 438
FaeI CATG 2 cut(s) 92, 863
FalI AAGNNNNNCTT 2 cut(s) 570, 602
FatI CATG 2 cut(s) 88, 859
FauNDI CATATG 1 cut(s) 574
FblI GTMKAC 1 cut(s) 84
Fnu4HI GCNGC 3 cut(s) 123, 126, 351
FriOI GRGCYC 3 cut(s) 27, 501, 834
Fsp4HI GCNGC 3 cut(s) 123, 126, 351
FspI TGCGCA 1 cut(s) 78
GlaI GCGC 1 cut(s) 78
GluI GCNGC 3 cut(s) 123, 126, 351
GsaI CCCAGC 1 cut(s) 758
GsuI CTGGAG 1 cut(s) 485
HapII CCGG 3 cut(s) 153, 219, 318
HhaI GCGC 1 cut(s) 79
Hin1II CATG 2 cut(s) 92, 863
Hin6I GCGC 1 cut(s) 77
HinP1I GCGC 1 cut(s) 77
HincII GTYRAC 4 cut(s) 85, 99, 328, 472
HindII GTYRAC 4 cut(s) 85, 99, 328, 472
HinfI GANTC 4 cut(s) 110, 251, 311, 710
HpaI GTTAAC 1 cut(s) 472
HpaII CCGG 3 cut(s) 153, 219, 318
HphI GGTGA 4 cut(s) 16, 167, 605, 749
Hpy166II GTNNAC 6 cut(s) 85, 99, 328, 374, 472, 607
Hpy188I TCNGA 2 cut(s) 340, 666
Hpy188III TCNNGA 1 cut(s) 412
Hpy8I GTNNAC 6 cut(s) 85, 99, 328, 374, 472, 607
Hpy99I CGWCG 1 cut(s) 86
HpyCH4III ACNGT 4 cut(s) 424, 469, 625, 650
HpyCH4IV ACGT 2 cut(s) 81, 324
HpyCH4V TGCA 7 cut(s) 140, 182, 259, 374, 646, 801, 859
HpyF10VI GCNNNNNNNGC 3 cut(s) 188, 635, 838
HpyF3I CTNAG 5 cut(s) 18, 192, 339, 417, 678
HpySE526I ACGT 2 cut(s) 81, 324
Hsp92II CATG 2 cut(s) 92, 863
HspAI GCGC 1 cut(s) 77
KspAI GTTAAC 1 cut(s) 472
Kzo9I GATC 4 cut(s) 450, 580, 843, 847
LmnI GCTCC 4 cut(s) 22, 30, 432, 504
Lsp1109I GCAGC 3 cut(s) 109, 112, 337
LweI GCATC 1 cut(s) 141
MaeII ACGT 2 cut(s) 81, 324
MaeIII GTNAC 1 cut(s) 343
MalI GATC 4 cut(s) 452, 582, 845, 849
MboI GATC 4 cut(s) 450, 580, 843, 847
MboII GAAGA 2 cut(s) 527, 590
MflI RGATCY 1 cut(s) 580
MhlI GDGCHC 6 cut(s) 27, 324, 376, 429, 501, 834
MluCI AATT 1 cut(s) 8
MlyI GAGTC 3 cut(s) 104, 260, 719
MnlI CCTC 7 cut(s) 13, 255, 305, 334, 391, 441, 511
MseI TTAA 1 cut(s) 471
MslI CAYNNNNRTG 1 cut(s) 858
MspA1I CMGCKG 3 cut(s) 17, 350, 638
MspI CCGG 3 cut(s) 153, 219, 318
MspR9I CCNGG 4 cut(s) 318, 319, 503, 602
MvaI CCWGG 2 cut(s) 503, 602
MwoI GCNNNNNNNGC 3 cut(s) 188, 635, 838
NciI CCSGG 2 cut(s) 318, 319
NcoI CCATGG 1 cut(s) 88
NdeI CATATG 1 cut(s) 574
NdeII GATC 4 cut(s) 450, 580, 843, 847
NlaIII CATG 2 cut(s) 92, 863
NlaIV GGNNCC 1 cut(s) 622
NmuCI GTSAC 1 cut(s) 343
NsbI TGCGCA 1 cut(s) 78
PfeI GAWTC 1 cut(s) 311
PflMI CCANNNNNTGG 2 cut(s) 590, 768
PinAI ACCGGT 1 cut(s) 218
PkrI GCNGC 3 cut(s) 124, 127, 352
PleI GAGTC 3 cut(s) 104, 259, 718
PpsI GAGTC 3 cut(s) 104, 259, 718
Psp124BI GAGCTC 2 cut(s) 27, 501
Psp6I CCWGG 2 cut(s) 501, 600
PspFI CCCAGC 1 cut(s) 754
PspGI CCWGG 2 cut(s) 501, 600
PspN4I GGNNCC 1 cut(s) 622
PspPI GGNCC 2 cut(s) 455, 771
PsuI RGATCY 1 cut(s) 580
PvuII CAGCTG 3 cut(s) 17, 350, 638
RsaI GTAC 3 cut(s) 233, 293, 608
RsaNI GTAC 3 cut(s) 232, 292, 607
RseI CAYNNNNRTG 1 cut(s) 858
SacI GAGCTC 2 cut(s) 27, 501
SalI GTCGAC 1 cut(s) 83
SaqAI TTAA 1 cut(s) 471
SatI GCNGC 3 cut(s) 123, 126, 351
Sau3AI GATC 4 cut(s) 450, 580, 843, 847
Sau96I GGNCC 2 cut(s) 455, 771
SchI GAGTC 3 cut(s) 104, 260, 719
ScrFI CCNGG 4 cut(s) 318, 319, 503, 602
SduI GDGCHC 6 cut(s) 27, 324, 376, 429, 501, 834
SfaNI GCATC 1 cut(s) 141
SinI GGWCC 2 cut(s) 455, 771
SmaI CCCGGG 1 cut(s) 319
SmiMI CAYNNNNRTG 1 cut(s) 858
SmlI CTYRAG 1 cut(s) 494
SmoI CTYRAG 1 cut(s) 494
Sse9I AATT 1 cut(s) 8
SstI GAGCTC 2 cut(s) 27, 501
StyD4I CCNGG 4 cut(s) 316, 317, 501, 600
StyI CCWWGG 2 cut(s) 88, 438
TaaI ACNGT 4 cut(s) 424, 469, 625, 650
TaiI ACGT 2 cut(s) 84, 327
TaqI TCGA 3 cut(s) 84, 507, 846
TaqII GACCGA 1 cut(s) 443
TasI AATT 1 cut(s) 8
TatI WGTACW 1 cut(s) 231
TfiI GAWTC 1 cut(s) 311
Tru1I TTAA 1 cut(s) 471
Tru9I TTAA 1 cut(s) 471
TscAI CASTG 1 cut(s) 790
TseFI GTSAC 1 cut(s) 343
TseI GCWGC 3 cut(s) 122, 125, 350
Tsp45I GTSAC 1 cut(s) 343
TspDTI ATGAA 3 cut(s) 201, 324, 591
TspGWI ACGGA 4 cut(s) 17, 152, 185, 419
TspMI CCCGGG 1 cut(s) 317
TspRI CASTG 1 cut(s) 790
Van91I CCANNNNNTGG 2 cut(s) 590, 768
VneI GTGCAC 1 cut(s) 372
VpaK11BI GGWCC 2 cut(s) 455, 771
XapI RAATTY 1 cut(s) 8
XcmI CCANNNNNNNNNTGG 1 cut(s) 562
XmaI CCCGGG 1 cut(s) 317
XmiI GTMKAC 1 cut(s) 84
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.