pycom05g24640

Cytochrome p450

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
pyrus_communis
Chr5
OG0002150
Physical Location & Seq
Reverse (-)
26151398 .. 26153167
1770 bp
Loading structure...
UTR
Exon/CDS
Intron
N/A

Sequence Viewer

Length: 1581 bp
ATGGTTGAAAGTTCAACTTTGACGACTTTCTGGCAGTGGAATCTGGCCCTTTATAAACCAGGCTTTTCAAGCTCCCTAAACATCACTACTGCTTTTGCCAACTTCAACCAAACTCTCTTCAAACAGTTTGAGGAAACCATGGAAACCCTTTACCTTGCTTTATCTTTGGGAACTGCTTTTTTAACCTTTATCATATTAGCATTCAAGGGCAAATCAGATGATGGCAAAAACCTTCCACCAGGGAGCATGGGGTGGCCTATTGTGGGTGAATCAATCGAGTTCCTGTTCGGGAAGCCGGAAAATTTTGTGTTCAAGAGGATGAGGAGGTACTCTCCTGACATCTTCAAGACCTATATTCTTGGGGAAAAAACTGCAGTGATTTGTGGTCCTAGTGGACACAAATTTCTCTTCTCCAATGAACAAAAGTACTTCACAGCTTTTCGACCACATTCGATGCAAAAGATGTTTCGTTCATACAAGGCTGCCGCCCCCACCGCTGCTGCTGCTCCTGCTGTGGCACAACCTTCACGTGATGAAGAAGCGAAAGTTATAAGATCGCCAGGGTTTTTGAAGCCGGAAGCATTGGTGAGGTACTTGGGGAAAATGGACTCTATCACTCAGGAACAGATGAAGGCCTATTGGGAAGGCAAAGATGTGGTCGAGGTCTACCCCTTGGCCAAGACCCTCACTCTAAGTCTTGCCTGCAGATTCTTCTTGGGTATCGATGAACCGGAGCGAATTTCAAGGCTTGTGAACAATTTTGATGATGTCACCGTTGGGATGCATTCACTTATTATCAACTTCCCAGGAACAACATTCTACAAGGCAACCAAAGCCGCAGACGCGCTGCGAAAGGAGTTGAGGATTGTGATTCAGGAGAAGAAGGCTGCGATGGCGGCAGGAGGTCCCATGCATGATATATTGTCACACATGATTGTGGCTAGTGACCCATCTGGTAAACACATGCCTGAGGCTGAGGTTGCGGATAAGATCATGGGTTTGCTCACAGCAGGATATAGCACTGTGGCTACTGCCATGACTTTCTTCATGAAATACGTTGGAGAGAGGCCAGACATCTATGCCAAGGTTCTAGCTGAGCACAAGGAGATAGCAGACTCAAAGAAGCCAGGAGCCTTCTTGGAATGGGAAGACATCAACAAAATGAAGTATTCATGGAATGTGTTGTATGAAGTGATGAGATTCACGCCGCCACTTCAAGGAACATTTAGAGAGGCCTTGACTGATTTTACCTACGCTGGTTACACCATTCCGAAGGGCTGGAAGGTATACTGGACTGTGAGTACAGTAAACATGAACTCAGAGTACTTCCCCAACCCAGAAAAGTTTGACCCATCAAGATATGATGACCTAAGTGTATTCCCAGCTTTCACATTTGTTCCATTTGGAGGAGGCCCAAGAATGTGCCCCGGGAAAGAGTACGCTCGCCTGGCAATCCTCACTTTCGTTCACAATGTGGTCAAGAGATTCAAATGGGAAGTGGTATTTCCTAAGGAGAAGATCACCGGCGACATGATGCCAACACCGGAAAAAGGACTTCCGGTTCGCCTTACCCGTCACTAG
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
No pathways identified.
Pfam Domains
Protein Families
p450

Protein Analysis

527

Amino Acids

59.53

Weight (kDa)

9.05

Isoelectric Point (pI)

40.97

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
p450 PF00067 79 - 501 2.3e-58 Cytochrome P450
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Publication-ready
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Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AanI TTATAA 2 cut(s) 54, 551
AccI GTMKAC 2 cut(s) 666, 1287
AccII CGCG 1 cut(s) 845
AciI CCGC 6 cut(s) 486, 495, 837, 896, 983, 1208
AcoI YGGCCR 1 cut(s) 675
AcsI RAATTY 3 cut(s) 301, 401, 738
AcvI CACGTG 1 cut(s) 530
AdeI CACNNNGTG 1 cut(s) 1474
AfaI GTAC 6 cut(s) 329, 428, 593, 1303, 1325, 1439
AfiI CCNNNNNNNGG 4 cut(s) 263, 1217, 1406, 1550
AjnI CCWGG 6 cut(s) 58, 238, 559, 805, 1126, 1446
AloI GAACNNNNNNTCC 2 cut(s) 1545, 1577
AluBI AGCT 4 cut(s) 72, 437, 1094, 1385
AluI AGCT 4 cut(s) 72, 437, 1094, 1385
Alw21I GWGCWC 1 cut(s) 1101
Ama87I CYCGRG 1 cut(s) 1427
AoxI GGCC 7 cut(s) 45, 254, 633, 675, 1067, 1233, 1411
ApeKI GCWGC 6 cut(s) 482, 497, 500, 503, 847, 887
ApoI RAATTY 3 cut(s) 301, 401, 738
ArsI GACNNNNNNTTYG 4 cut(s) 642, 674, 1545, 1577
AspLEI GCGC 1 cut(s) 847
AspS9I GGNCC 4 cut(s) 46, 386, 905, 1412
AsuC2I CCSGG 2 cut(s) 1428, 1429
AsuHPI GGTGA 4 cut(s) 278, 598, 763, 1513
AvaI CYCGRG 1 cut(s) 1427
AvaII GGWCC 2 cut(s) 386, 905
AxyI CCTNAGG 2 cut(s) 969, 1509
BaeGI GKGCMC 1 cut(s) 1427
BalI TGGCCA 1 cut(s) 677
BbrPI CACGTG 1 cut(s) 530
BbsI GAAGAC 1 cut(s) 1155
Bbv12I GWGCWC 1 cut(s) 1101
BbvCI CCTCAGC 1 cut(s) 975
BbvI GCAGC 6 cut(s) 469, 484, 487, 490, 834, 874
BccI CCATC 4 cut(s) 215, 886, 958, 1360
BciT130I CCWGG 6 cut(s) 60, 240, 561, 807, 1128, 1448
BcnI CCSGG 2 cut(s) 1428, 1429
BfaI CTAG 4 cut(s) 390, 942, 1091, 1579
BfmI CTRYAG 2 cut(s) 372, 703
BlpI GCTNAGC 1 cut(s) 1095
BmcAI AGTACT 2 cut(s) 428, 1325
Bme1390I CCNGG 8 cut(s) 60, 240, 561, 807, 1128, 1428, 1429, 1448
Bme18I GGWCC 2 cut(s) 386, 905
BmeT110I CYCGRG 1 cut(s) 1427
BmgT120I GGNCC 4 cut(s) 46, 386, 905, 1412
BmiI GGNNCC 2 cut(s) 907, 1132
BmrFI CCNGG 8 cut(s) 60, 240, 561, 807, 1128, 1428, 1429, 1448
BmsI GCATC 3 cut(s) 444, 771, 1524
BpiI GAAGAC 1 cut(s) 1155
BplI GAGNNNNNCTC 2 cut(s) 316, 348
Bpu10I CCTNAGC 1 cut(s) 975
Bpu1102I GCTNAGC 1 cut(s) 1095
BpuMI CCSGG 2 cut(s) 1428, 1429
Bsa29I ATCGAT 1 cut(s) 723
BsaAI YACGTR 1 cut(s) 530
BsaJI CCNNGG 8 cut(s) 138, 239, 560, 672, 805, 1083, 1426, 1427
BsaWI WCCGGW 3 cut(s) 730, 1543, 1558
Bsc4I CCNNNNNNNGG 4 cut(s) 263, 1217, 1406, 1550
Bse118I RCCGGY 1 cut(s) 1523
Bse1I ACTGG 1 cut(s) 1295
Bse21I CCTNAGG 2 cut(s) 969, 1509
BseBI CCWGG 6 cut(s) 60, 240, 561, 807, 1128, 1448
BseCI ATCGAT 1 cut(s) 723
BseDI CCNNGG 8 cut(s) 138, 239, 560, 672, 805, 1083, 1426, 1427
BseGI GGATG 2 cut(s) 324, 786
BseLI CCNNNNNNNGG 4 cut(s) 263, 1217, 1406, 1550
BseMII CTCAG 5 cut(s) 632, 960, 966, 1086, 1332
BseNI ACTGG 1 cut(s) 1295
BseRI GAGGAG 2 cut(s) 337, 1422
BseSI GKGCMC 1 cut(s) 1427
BseXI GCAGC 6 cut(s) 469, 484, 487, 490, 834, 874
BseYI CCCAGC 1 cut(s) 1381
Bsh1236I CGCG 1 cut(s) 845
BshFI GGCC 7 cut(s) 47, 256, 635, 677, 1069, 1235, 1413
BshVI ATCGAT 1 cut(s) 723
BsiHKAI GWGCWC 1 cut(s) 1101
BsiHKCI CYCGRG 1 cut(s) 1427
BsiSI CCGG 7 cut(s) 296, 575, 731, 1428, 1524, 1544, 1559
BslFI GGGAC 1 cut(s) 891
BslI CCNNNNNNNGG 4 cut(s) 263, 1217, 1406, 1550
BsmFI GGGAC 1 cut(s) 891
BsmI GAATGC 2 cut(s) 200, 784
BsnI GGCC 7 cut(s) 47, 256, 635, 677, 1069, 1235, 1413
BsoBI CYCGRG 1 cut(s) 1427
Bsp1286I GDGCHC 2 cut(s) 1101, 1427
Bsp143I GATC 3 cut(s) 554, 990, 1518
Bsp1720I GCTNAGC 1 cut(s) 1095
Bsp19I CCATGG 1 cut(s) 138
BspACI CCGC 6 cut(s) 486, 495, 837, 896, 983, 1208
BspANI GGCC 7 cut(s) 47, 256, 635, 677, 1069, 1235, 1413
BspCNI CTCAG 5 cut(s) 631, 961, 967, 1087, 1331
BspDI ATCGAT 1 cut(s) 723
BspFNI CGCG 1 cut(s) 845
BspHI TCATGA 1 cut(s) 1047
BspLI GGNNCC 2 cut(s) 907, 1132
BspMAI CTGCAG 2 cut(s) 376, 707
BsrFI RCCGGY 1 cut(s) 1523
BsrI ACTGG 1 cut(s) 1295
BssAI RCCGGY 1 cut(s) 1523
BssECI CCNNGG 8 cut(s) 138, 239, 560, 672, 805, 1083, 1426, 1427
BssMI GATC 3 cut(s) 554, 990, 1518
BssNAI GTATAC 1 cut(s) 1288
BssT1I CCWWGG 3 cut(s) 138, 672, 1083
Bst1107I GTATAC 1 cut(s) 1288
Bst2UI CCWGG 6 cut(s) 60, 240, 561, 807, 1128, 1448
Bst4CI ACNGT 5 cut(s) 126, 775, 1024, 1297, 1306
Bst6I CTCTTC 2 cut(s) 122, 413
BstBAI YACGTR 1 cut(s) 530
BstC8I GCNNGC 2 cut(s) 703, 1444
BstDEI CTNAG 8 cut(s) 618, 692, 969, 975, 1095, 1318, 1370, 1509
BstDSI CCRYGG 1 cut(s) 138
BstF5I GGATG 2 cut(s) 324, 786
BstFNI CGCG 1 cut(s) 845
BstHHI GCGC 1 cut(s) 847
BstKTI GATC 3 cut(s) 557, 993, 1521
BstMBI GATC 3 cut(s) 554, 990, 1518
BstNI CCWGG 6 cut(s) 60, 240, 561, 807, 1128, 1448
BstNSI RCATGY 1 cut(s) 967
BstSCI CCNGG 8 cut(s) 58, 238, 559, 805, 1126, 1426, 1427, 1446
BstSFI CTRYAG 2 cut(s) 372, 703
BstSLI GKGCMC 1 cut(s) 1427
BstUI CGCG 1 cut(s) 845
BstV1I GCAGC 6 cut(s) 469, 484, 487, 490, 834, 874
BstV2I GAAGAC 1 cut(s) 1155
BstZ17I GTATAC 1 cut(s) 1288
Bsu15I ATCGAT 1 cut(s) 723
Bsu36I CCTNAGG 2 cut(s) 969, 1509
BsuRI GGCC 7 cut(s) 47, 256, 635, 677, 1069, 1235, 1413
BsuTUI ATCGAT 1 cut(s) 723
BtgI CCRYGG 1 cut(s) 138
BtgZI GCGATG 1 cut(s) 905
BtsCI GGATG 2 cut(s) 324, 786
BtsI GCAGTG 2 cut(s) 41, 381
BtsIMutI CAGTG 3 cut(s) 41, 381, 1020
Cac8I GCNNGC 2 cut(s) 703, 1444
CciI TCATGA 1 cut(s) 1047
CfoI GCGC 1 cut(s) 847
Cfr10I RCCGGY 1 cut(s) 1523
Cfr13I GGNCC 4 cut(s) 46, 386, 905, 1412
Cfr9I CCCGGG 1 cut(s) 1427
ClaI ATCGAT 1 cut(s) 723
CseI GACGC 1 cut(s) 851
Csp6I GTAC 6 cut(s) 328, 427, 592, 1302, 1324, 1438
CviQI GTAC 6 cut(s) 328, 427, 592, 1302, 1324, 1438
DdeI CTNAG 8 cut(s) 618, 692, 969, 975, 1095, 1318, 1370, 1509
DpnI GATC 3 cut(s) 556, 992, 1520
DpnII GATC 3 cut(s) 554, 990, 1518
DraIII CACNNNGTG 1 cut(s) 1474
EaeI YGGCCR 1 cut(s) 675
Eam1104I CTCTTC 2 cut(s) 122, 413
EarI CTCTTC 2 cut(s) 122, 413
Eco130I CCWWGG 3 cut(s) 138, 672, 1083
Eco147I AGGCCT 2 cut(s) 635, 1235
Eco47I GGWCC 2 cut(s) 386, 905
Eco72I CACGTG 1 cut(s) 530
Eco81I CCTNAGG 2 cut(s) 969, 1509
Eco88I CYCGRG 1 cut(s) 1427
EcoO109I RGGNCCY 1 cut(s) 905
EcoRII CCWGG 6 cut(s) 58, 238, 559, 805, 1126, 1446
EcoT14I CCWWGG 3 cut(s) 138, 672, 1083
EcoT22I ATGCAT 2 cut(s) 786, 915
ErhI CCWWGG 3 cut(s) 138, 672, 1083
FalI AAGNNNNNCTT 1 cut(s) 33
FaqI GGGAC 1 cut(s) 891
FblI GTMKAC 2 cut(s) 666, 1287
FokI GGATG 2 cut(s) 331, 793
FspBI CTAG 4 cut(s) 390, 942, 1091, 1579
GlaI GCGC 1 cut(s) 846
GsaI CCCAGC 1 cut(s) 1385
HaeIII GGCC 7 cut(s) 47, 256, 635, 677, 1069, 1235, 1413
HapII CCGG 7 cut(s) 296, 575, 731, 1428, 1524, 1544, 1559
HgaI GACGC 1 cut(s) 851
HhaI GCGC 1 cut(s) 847
Hin6I GCGC 1 cut(s) 845
HinP1I GCGC 1 cut(s) 845
HinfI GANTC 8 cut(s) 40, 269, 608, 708, 871, 1115, 1200, 1485
HpaII CCGG 7 cut(s) 296, 575, 731, 1428, 1524, 1544, 1559
HphI GGTGA 4 cut(s) 278, 598, 763, 1513
Hpy166II GTNNAC 7 cut(s) 395, 667, 754, 959, 1288, 1309, 1468
Hpy188I TCNGA 3 cut(s) 217, 1272, 1321
Hpy188III TCNNGA 9 cut(s) 289, 313, 335, 346, 620, 875, 1048, 1356, 1480
Hpy8I GTNNAC 7 cut(s) 395, 667, 754, 959, 1288, 1309, 1468
HpyAV CCTTC 8 cut(s) 242, 534, 625, 638, 877, 1144, 1267, 1276
HpyCH4III ACNGT 5 cut(s) 126, 775, 1024, 1297, 1306
HpyCH4IV ACGT 2 cut(s) 529, 1056
HpyCH4V TGCA 5 cut(s) 374, 457, 705, 784, 913
HpyF3I CTNAG 8 cut(s) 618, 692, 969, 975, 1095, 1318, 1370, 1509
HpySE526I ACGT 2 cut(s) 529, 1056
HspAI GCGC 1 cut(s) 845
Kzo9I GATC 3 cut(s) 554, 990, 1518
LmnI GCTCC 5 cut(s) 77, 243, 511, 733, 1130
Lsp1109I GCAGC 6 cut(s) 469, 484, 487, 490, 834, 874
LweI GCATC 3 cut(s) 444, 771, 1524
MaeI CTAG 4 cut(s) 390, 942, 1091, 1579
MaeII ACGT 2 cut(s) 529, 1056
MaeIII GTNAC 5 cut(s) 769, 924, 944, 1259, 1574
MalI GATC 3 cut(s) 556, 992, 1520
MboI GATC 3 cut(s) 554, 990, 1518
MboII GAAGA 9 cut(s) 109, 334, 400, 548, 703, 892, 1036, 1160, 1528
MhlI GDGCHC 2 cut(s) 1101, 1427
MlsI TGGCCA 1 cut(s) 677
MluCI AATT 4 cut(s) 301, 401, 738, 757
MluNI TGGCCA 1 cut(s) 677
MlyI GAGTC 2 cut(s) 602, 1109
MmeI TCCRAC 1 cut(s) 1039
Mox20I TGGCCA 1 cut(s) 677
Mph1103I ATGCAT 2 cut(s) 786, 915
MscI TGGCCA 1 cut(s) 677
MseI TTAA 1 cut(s) 182
MslI CAYNNNNRTG 1 cut(s) 935
Msp20I TGGCCA 1 cut(s) 677
MspA1I CMGCKG 1 cut(s) 497
MspI CCGG 7 cut(s) 296, 575, 731, 1428, 1524, 1544, 1559
MspR9I CCNGG 8 cut(s) 60, 240, 561, 807, 1128, 1428, 1429, 1448
Mva1269I GAATGC 2 cut(s) 200, 784
MvaI CCWGG 6 cut(s) 60, 240, 561, 807, 1128, 1448
MvnI CGCG 1 cut(s) 845
NciI CCSGG 2 cut(s) 1428, 1429
NcoI CCATGG 1 cut(s) 138
NdeII GATC 3 cut(s) 554, 990, 1518
NlaIV GGNNCC 2 cut(s) 907, 1132
NmuCI GTSAC 4 cut(s) 769, 924, 944, 1574
NsiI ATGCAT 2 cut(s) 786, 915
NspI RCATGY 1 cut(s) 967
PagI TCATGA 1 cut(s) 1047
PceI AGGCCT 2 cut(s) 635, 1235
PcsI WCGNNNNNNNCGW 1 cut(s) 1570
PctI GAATGC 2 cut(s) 200, 784
PfeI GAWTC 6 cut(s) 40, 269, 708, 871, 1200, 1485
PleI GAGTC 2 cut(s) 602, 1109
PmaCI CACGTG 1 cut(s) 530
PmlI CACGTG 1 cut(s) 530
PpsI GAGTC 2 cut(s) 602, 1109
Ppu21I YACGTR 1 cut(s) 530
PpuMI RGGWCCY 1 cut(s) 905
PsiI TTATAA 2 cut(s) 54, 551
Psp5II RGGWCCY 1 cut(s) 905
Psp6I CCWGG 6 cut(s) 58, 238, 559, 805, 1126, 1446
PspCI CACGTG 1 cut(s) 530
PspFI CCCAGC 1 cut(s) 1381
PspGI CCWGG 6 cut(s) 58, 238, 559, 805, 1126, 1446
PspN4I GGNNCC 2 cut(s) 907, 1132
PspPI GGNCC 4 cut(s) 46, 386, 905, 1412
PspPPI RGGWCCY 1 cut(s) 905
PsrI GAACNNNNNNTAC 2 cut(s) 1307, 1339
PstI CTGCAG 2 cut(s) 376, 707
RsaI GTAC 6 cut(s) 329, 428, 593, 1303, 1325, 1439
RsaNI GTAC 6 cut(s) 328, 427, 592, 1302, 1324, 1438
RseI CAYNNNNRTG 1 cut(s) 935
SaqAI TTAA 1 cut(s) 182
Sau3AI GATC 3 cut(s) 554, 990, 1518
Sau96I GGNCC 4 cut(s) 46, 386, 905, 1412
ScaI AGTACT 2 cut(s) 428, 1325
SchI GAGTC 2 cut(s) 602, 1109
ScrFI CCNGG 8 cut(s) 60, 240, 561, 807, 1128, 1428, 1429, 1448
SduI GDGCHC 2 cut(s) 1101, 1427
SfaNI GCATC 3 cut(s) 444, 771, 1524
SfcI CTRYAG 2 cut(s) 372, 703
SgrAI CRCCGGYG 1 cut(s) 1523
SinI GGWCC 2 cut(s) 386, 905
SmaI CCCGGG 1 cut(s) 1429
SmiMI CAYNNNNRTG 1 cut(s) 935
Sse9I AATT 4 cut(s) 301, 401, 738, 757
SseBI AGGCCT 2 cut(s) 635, 1235
SsiI CCGC 6 cut(s) 486, 495, 837, 896, 983, 1208
SspMI CTAG 4 cut(s) 390, 942, 1091, 1579
StuI AGGCCT 2 cut(s) 635, 1235
StyD4I CCNGG 8 cut(s) 58, 238, 559, 805, 1126, 1426, 1427, 1446
StyI CCWWGG 3 cut(s) 138, 672, 1083
TaaI ACNGT 5 cut(s) 126, 775, 1024, 1297, 1306
TaiI ACGT 2 cut(s) 532, 1059
TaqI TCGA 5 cut(s) 276, 442, 452, 660, 723
TasI AATT 4 cut(s) 301, 401, 738, 757
TatI WGTACW 3 cut(s) 426, 1301, 1323
TauI GCSGC 4 cut(s) 488, 839, 899, 1210
TfiI GAWTC 6 cut(s) 40, 269, 708, 871, 1200, 1485
Tru1I TTAA 1 cut(s) 182
Tru9I TTAA 1 cut(s) 182
TscAI CASTG 3 cut(s) 41, 381, 1027
TseFI GTSAC 4 cut(s) 769, 924, 944, 1574
TseI GCWGC 6 cut(s) 482, 497, 500, 503, 847, 887
Tsp45I GTSAC 4 cut(s) 769, 924, 944, 1574
TspMI CCCGGG 1 cut(s) 1427
TspRI CASTG 3 cut(s) 41, 381, 1027
VpaK11BI GGWCC 2 cut(s) 386, 905
XapI RAATTY 3 cut(s) 301, 401, 738
XceI RCATGY 1 cut(s) 967
XmaI CCCGGG 1 cut(s) 1427
XmiI GTMKAC 2 cut(s) 666, 1287
XspI CTAG 4 cut(s) 390, 942, 1091, 1579
ZrmI AGTACT 2 cut(s) 428, 1325
Zsp2I ATGCAT 2 cut(s) 786, 915
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.