pycom05g26510

Belongs to the beta-ketoacyl-ACP synthases family

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
pyrus_communis
Chr5
OG0003245
Physical Location & Seq
Reverse (-)
27759329 .. 27760692
1364 bp
Loading structure...
UTR
Exon/CDS
Intron
N/A

Sequence Viewer

Length: 819 bp
ATGCAGTCCCTCCAATCCTCCACTCTCCGGCCCGCTCCGCTCAGCCCGATTCGGAAACGGACCCCACATTTCCCCAATGTCACTACCAGAACAGCCAACTCCTCCAGGCGAATCTCATTCATATCCGCCTCCTCCGCCGTGTCCGCCCCTAAGCGCGAGATGGACCCGAAGAAGCGGGTTGTCATCACCGGAATGGGCCTCGTCTCCGTCTTCGGCAACGACGTCAATACTTACTACGACAAGCTCCTCGCCGGAGAGAGCGGTGTGGGTCCAATTGACCGGTTTGATGCGTCCAAGTTTCCGACCCGGTTCGGGGGCCAGATCCGCGGGTTCTCATCGGAGGGGTACATTGATGGGAAGAACGATCGACGGCTCGACGATTGCCTTCGGTACTCGATTGTTGCTGGGAAGAAAGCTCTCGAGGATGCTGATCTTGGCGCCGATCAGCGCTCAAAGCTTGATAAATCGCGGACTGGGGTGCTTGTTGGATCTGGAATGGGTGGTCTTACTGTCTTTTCTGATGGTGTTCATGCACTGATAGAGAAAGGTCACAGGAAAATTACCCCCTTTTTCATTCCTTATGCTATTACAAACATGGGGTCTGCGTTGCTTGCTATTGATCTGGGTTTTATGGGACCCAATTATTCCATTTCGACTGCTTGTGCTACTTCAAATTATTGCTTCTATGCTGCTGCAAATCACATTCGTCGGGGTGAAGCTGATTTGATGATTGCTGGCGGAACTGAAGCTGCCATAATTCCTGTTGGGGTGGGAGGGTTTGTTGCATGCAGGGCCTTGTCTCAGAGAATGGGGTTATGA
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
ko00061 ko00780 ko01100 ko01212 map00061 map00780 map01100 map01212
Pfam Domains
Protein Families
Ketoacyl-synt_C ketoacyl-synt

Protein Analysis

273

Amino Acids

29.09

Weight (kDa)

9.66

Isoelectric Point (pI)

45.18

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
ketoacyl-synt PF00109 58 - 268 1.5e-45 Beta-ketoacyl synthase, N-terminal domain
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Publication-ready
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Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AatII GACGTC 1 cut(s) 225
AccB1I GGYRCC 1 cut(s) 437
AccBSI CCGCTC 3 cut(s) 35, 40, 261
AccII CGCG 3 cut(s) 156, 327, 469
AclWI GGATC 2 cut(s) 316, 496
AcuI CTGAAG 1 cut(s) 765
AcyI GRCGYC 2 cut(s) 222, 438
AfaI GTAC 2 cut(s) 347, 392
AfeI AGCGCT 1 cut(s) 449
AfiI CCNNNNNNNGG 3 cut(s) 27, 312, 313
AgeI ACCGGT 1 cut(s) 279
AgsI TTSAA 1 cut(s) 672
AjnI CCWGG 1 cut(s) 104
AluBI AGCT 5 cut(s) 244, 416, 457, 719, 749
AluI AGCT 5 cut(s) 244, 416, 457, 719, 749
Alw26I GTCTC 2 cut(s) 208, 804
AlwI GGATC 2 cut(s) 316, 496
Ama87I CYCGRG 1 cut(s) 419
Aor51HI AGCGCT 1 cut(s) 449
AoxI GGCC 4 cut(s) 29, 196, 316, 792
ApeKI GCWGC 3 cut(s) 689, 692, 749
ArsI GACNNNNNNTTYG 2 cut(s) 584, 616
AsiGI ACCGGT 1 cut(s) 279
AspLEI GCGC 3 cut(s) 156, 440, 450
AspS9I GGNCC 8 cut(s) 30, 60, 163, 196, 269, 316, 635, 792
AsuC2I CCSGG 1 cut(s) 307
AsuHPI GGTGA 2 cut(s) 178, 725
AvaI CYCGRG 1 cut(s) 419
AvaII GGWCC 4 cut(s) 60, 163, 269, 635
BanI GGYRCC 1 cut(s) 437
BbsI GAAGAC 1 cut(s) 202
BbvI GCAGC 3 cut(s) 676, 679, 736
BccI CCATC 3 cut(s) 154, 347, 515
BceAI ACGGC 2 cut(s) 122, 386
BciT130I CCWGG 1 cut(s) 106
BcnI CCSGG 1 cut(s) 307
BcoDI GTCTC 2 cut(s) 208, 804
BfoI RGCGCY 2 cut(s) 441, 451
BisI GCNGC 3 cut(s) 690, 693, 750
BlpI GCTNAGC 1 cut(s) 41
BlsI GCNGC 3 cut(s) 691, 694, 751
Bme1390I CCNGG 2 cut(s) 106, 307
Bme18I GGWCC 4 cut(s) 60, 163, 269, 635
BmeT110I CYCGRG 1 cut(s) 419
BmgT120I GGNCC 8 cut(s) 30, 60, 163, 196, 269, 316, 635, 792
BmiI GGNNCC 7 cut(s) 62, 165, 270, 317, 439, 636, 637
BmrFI CCNGG 2 cut(s) 106, 307
BmrI ACTGGG 1 cut(s) 483
BmsI GCATC 2 cut(s) 277, 415
BmuI ACTGGG 1 cut(s) 483
BpiI GAAGAC 1 cut(s) 202
BpmI CTGGAG 1 cut(s) 88
Bpu10I CCTNAGC 1 cut(s) 150
Bpu1102I GCTNAGC 1 cut(s) 41
BpuMI CCSGG 1 cut(s) 307
BsaBI GATNNNNATC 1 cut(s) 429
BsaHI GRCGYC 2 cut(s) 222, 438
BsaJI CCNNGG 1 cut(s) 325
BsaWI WCCGGW 2 cut(s) 188, 279
BsaXI ACNNNNNCTCC 2 cut(s) 765, 795
Bsc4I CCNNNNNNNGG 3 cut(s) 27, 312, 313
Bse118I RCCGGY 1 cut(s) 279
Bse1I ACTGG 1 cut(s) 478
Bse8I GATNNNNATC 1 cut(s) 429
BseBI CCWGG 1 cut(s) 106
BseDI CCNNGG 1 cut(s) 325
BseGI GGATG 1 cut(s) 430
BseJI GATNNNNATC 1 cut(s) 429
BseLI CCNNNNNNNGG 3 cut(s) 27, 312, 313
BseMII CTCAG 2 cut(s) 55, 815
BseNI ACTGG 1 cut(s) 478
BseRI GAGGAG 3 cut(s) 91, 121, 236
BseXI GCAGC 3 cut(s) 676, 679, 736
BseYI CCCAGC 1 cut(s) 404
Bsh1236I CGCG 3 cut(s) 156, 327, 469
Bsh1285I CGRYCG 1 cut(s) 367
BshFI GGCC 4 cut(s) 31, 198, 318, 794
BshNI GGYRCC 1 cut(s) 437
BshTI ACCGGT 1 cut(s) 279
BsiEI CGRYCG 1 cut(s) 367
BsiHKCI CYCGRG 1 cut(s) 419
BsiSI CCGG 5 cut(s) 28, 189, 252, 280, 307
BslFI GGGAC 1 cut(s) 648
BslI CCNNNNNNNGG 3 cut(s) 27, 312, 313
BsmAI GTCTC 2 cut(s) 208, 804
BsmBI CGTCTC 1 cut(s) 208
BsmFI GGGAC 1 cut(s) 648
BsnI GGCC 4 cut(s) 31, 198, 318, 794
BsoBI CYCGRG 1 cut(s) 419
Bsp143I GATC 6 cut(s) 321, 364, 430, 442, 488, 619
Bsp1720I GCTNAGC 1 cut(s) 41
BspANI GGCC 4 cut(s) 31, 198, 318, 794
BspCNI CTCAG 2 cut(s) 54, 814
BspFNI CGCG 3 cut(s) 156, 327, 469
BspLI GGNNCC 7 cut(s) 62, 165, 270, 317, 439, 636, 637
BspPI GGATC 2 cut(s) 316, 496
BspT107I GGYRCC 1 cut(s) 437
BsrBI CCGCTC 3 cut(s) 35, 40, 261
BsrFI RCCGGY 1 cut(s) 279
BsrI ACTGG 1 cut(s) 478
BssAI RCCGGY 1 cut(s) 279
BssECI CCNNGG 1 cut(s) 325
BssMI GATC 6 cut(s) 321, 364, 430, 442, 488, 619
BssNI GRCGYC 2 cut(s) 222, 438
Bst2UI CCWGG 1 cut(s) 106
Bst4CI ACNGT 1 cut(s) 511
BstACI GRCGYC 2 cut(s) 222, 438
BstC8I GCNNGC 4 cut(s) 33, 612, 736, 787
BstDEI CTNAG 3 cut(s) 41, 150, 801
BstDSI CCRYGG 1 cut(s) 325
BstF5I GGATG 1 cut(s) 430
BstFNI CGCG 3 cut(s) 156, 327, 469
BstH2I RGCGCY 2 cut(s) 441, 451
BstHHI GCGC 3 cut(s) 156, 440, 450
BstKTI GATC 6 cut(s) 324, 367, 433, 445, 491, 622
BstMAI GTCTC 2 cut(s) 208, 804
BstMBI GATC 6 cut(s) 321, 364, 430, 442, 488, 619
BstMCI CGRYCG 1 cut(s) 367
BstMWI GCNNNNNNNGC 7 cut(s) 37, 134, 143, 324, 454, 611, 791
BstNI CCWGG 1 cut(s) 106
BstNSI RCATGY 1 cut(s) 789
BstSCI CCNGG 2 cut(s) 104, 305
BstUI CGCG 3 cut(s) 156, 327, 469
BstV1I GCAGC 3 cut(s) 676, 679, 736
BstV2I GAAGAC 1 cut(s) 202
BstX2I RGATCY 2 cut(s) 321, 488
BstYI RGATCY 2 cut(s) 321, 488
BsuRI GGCC 4 cut(s) 31, 198, 318, 794
BtgI CCRYGG 1 cut(s) 325
BtsCI GGATG 1 cut(s) 430
BtsIMutI CAGTG 1 cut(s) 533
Cac8I GCNNGC 4 cut(s) 33, 612, 736, 787
CfoI GCGC 3 cut(s) 156, 440, 450
Cfr10I RCCGGY 1 cut(s) 279
Cfr13I GGNCC 8 cut(s) 30, 60, 163, 196, 269, 316, 635, 792
Cfr42I CCGCGG 1 cut(s) 328
CseI GACGC 1 cut(s) 279
Csp6I GTAC 2 cut(s) 346, 391
CspAI ACCGGT 1 cut(s) 279
CviAII CATG 3 cut(s) 530, 595, 786
CviQI GTAC 2 cut(s) 346, 391
DdeI CTNAG 3 cut(s) 41, 150, 801
DinI GGCGCC 1 cut(s) 439
DpnI GATC 6 cut(s) 323, 366, 432, 444, 490, 621
DpnII GATC 6 cut(s) 321, 364, 430, 442, 488, 619
EciI GGCGGA 4 cut(s) 115, 124, 133, 753
Eco47I GGWCC 4 cut(s) 60, 163, 269, 635
Eco47III AGCGCT 1 cut(s) 449
Eco57I CTGAAG 1 cut(s) 765
Eco88I CYCGRG 1 cut(s) 419
EcoO109I RGGNCCY 2 cut(s) 635, 792
EcoRII CCWGG 1 cut(s) 104
EgeI GGCGCC 1 cut(s) 439
EheI GGCGCC 1 cut(s) 439
Esp3I CGTCTC 1 cut(s) 208
FaeI CATG 3 cut(s) 533, 598, 789
FaiI YATR 9 cut(s) 122, 531, 582, 596, 632, 687, 755, 787, 817
FaqI GGGAC 1 cut(s) 648
FatI CATG 3 cut(s) 529, 594, 785
FauI CCCGC 3 cut(s) 40, 168, 320
Fnu4HI GCNGC 3 cut(s) 690, 693, 750
FokI GGATG 1 cut(s) 437
Fsp4HI GCNGC 3 cut(s) 690, 693, 750
GlaI GCGC 3 cut(s) 155, 439, 449
GluI GCNGC 3 cut(s) 690, 693, 750
GsaI CCCAGC 1 cut(s) 408
GsuI CTGGAG 1 cut(s) 88
HaeII RGCGCY 2 cut(s) 441, 451
HaeIII GGCC 4 cut(s) 31, 198, 318, 794
HapII CCGG 5 cut(s) 28, 189, 252, 280, 307
HgaI GACGC 1 cut(s) 279
HhaI GCGC 3 cut(s) 156, 440, 450
Hin1I GRCGYC 2 cut(s) 222, 438
Hin1II CATG 3 cut(s) 533, 598, 789
Hin6I GCGC 3 cut(s) 154, 438, 448
HinP1I GCGC 3 cut(s) 154, 438, 448
HindIII AAGCTT 1 cut(s) 455
HinfI GANTC 2 cut(s) 49, 111
HpaII CCGG 5 cut(s) 28, 189, 252, 280, 307
HphI GGTGA 2 cut(s) 178, 725
Hpy188I TCNGA 5 cut(s) 54, 303, 340, 520, 804
Hpy188III TCNNGA 2 cut(s) 419, 492
Hpy99I CGWCG 4 cut(s) 224, 372, 380, 711
HpyAV CCTTC 1 cut(s) 395
HpyCH4III ACNGT 1 cut(s) 511
HpyCH4IV ACGT 1 cut(s) 222
HpyCH4V TGCA 5 cut(s) 4, 533, 695, 785, 789
HpyF10VI GCNNNNNNNGC 7 cut(s) 37, 134, 143, 324, 454, 611, 791
HpyF3I CTNAG 3 cut(s) 41, 150, 801
HpySE526I ACGT 1 cut(s) 222
Hsp92I GRCGYC 2 cut(s) 222, 438
Hsp92II CATG 3 cut(s) 533, 598, 789
HspAI GCGC 3 cut(s) 154, 438, 448
KasI GGCGCC 1 cut(s) 437
KflI GGGWCCC 1 cut(s) 635
KspI CCGCGG 1 cut(s) 328
Kzo9I GATC 6 cut(s) 321, 364, 430, 442, 488, 619
LmnI GCTCC 2 cut(s) 40, 249
Lsp1109I GCAGC 3 cut(s) 676, 679, 736
LweI GCATC 2 cut(s) 277, 415
MaeII ACGT 1 cut(s) 222
MaeIII GTNAC 2 cut(s) 79, 548
MalI GATC 6 cut(s) 323, 366, 432, 444, 490, 621
MbiI CCGCTC 3 cut(s) 35, 40, 261
MboI GATC 6 cut(s) 321, 364, 430, 442, 488, 619
MboII GAAGA 4 cut(s) 181, 202, 370, 421
MfeI CAATTG 1 cut(s) 273
MflI RGATCY 2 cut(s) 321, 488
MluCI AATT 5 cut(s) 273, 558, 640, 673, 756
Mly113I GGCGCC 1 cut(s) 438
MmeI TCCRAC 2 cut(s) 326, 466
MslI CAYNNNNRTG 1 cut(s) 191
MspA1I CMGCKG 1 cut(s) 327
MspI CCGG 5 cut(s) 28, 189, 252, 280, 307
MspR9I CCNGG 2 cut(s) 106, 307
MunI CAATTG 1 cut(s) 273
MvaI CCWGG 1 cut(s) 106
MvnI CGCG 3 cut(s) 156, 327, 469
MwoI GCNNNNNNNGC 7 cut(s) 37, 134, 143, 324, 454, 611, 791
NarI GGCGCC 1 cut(s) 438
NciI CCSGG 1 cut(s) 307
NdeII GATC 6 cut(s) 321, 364, 430, 442, 488, 619
NlaIII CATG 3 cut(s) 533, 598, 789
NlaIV GGNNCC 7 cut(s) 62, 165, 270, 317, 439, 636, 637
NmuCI GTSAC 2 cut(s) 79, 548
NspI RCATGY 1 cut(s) 789
PaeI GCATGC 1 cut(s) 789
PaeR7I CTCGAG 1 cut(s) 419
PcsI WCGNNNNNNNCGW 1 cut(s) 219
PfeI GAWTC 2 cut(s) 49, 111
PinAI ACCGGT 1 cut(s) 279
PkrI GCNGC 3 cut(s) 691, 694, 751
Ple19I CGATCG 1 cut(s) 367
PluTI GGCGCC 1 cut(s) 441
PpuMI RGGWCCY 1 cut(s) 635
Psp5II RGGWCCY 1 cut(s) 635
Psp6I CCWGG 1 cut(s) 104
PspFI CCCAGC 1 cut(s) 404
PspGI CCWGG 1 cut(s) 104
PspN4I GGNNCC 7 cut(s) 62, 165, 270, 317, 439, 636, 637
PspPI GGNCC 8 cut(s) 30, 60, 163, 196, 269, 316, 635, 792
PspPPI RGGWCCY 1 cut(s) 635
PsuI RGATCY 2 cut(s) 321, 488
PvuI CGATCG 1 cut(s) 367
RsaI GTAC 2 cut(s) 347, 392
RsaNI GTAC 2 cut(s) 346, 391
RseI CAYNNNNRTG 1 cut(s) 191
SacII CCGCGG 1 cut(s) 328
SatI GCNGC 3 cut(s) 690, 693, 750
Sau3AI GATC 6 cut(s) 321, 364, 430, 442, 488, 619
Sau96I GGNCC 8 cut(s) 30, 60, 163, 196, 269, 316, 635, 792
ScrFI CCNGG 2 cut(s) 106, 307
SetI ASST 7 cut(s) 225, 246, 418, 459, 550, 721, 751
SfaNI GCATC 2 cut(s) 277, 415
SfoI GGCGCC 1 cut(s) 439
Sfr274I CTCGAG 1 cut(s) 419
Sfr303I CCGCGG 1 cut(s) 328
SgrBI CCGCGG 1 cut(s) 328
SinI GGWCC 4 cut(s) 60, 163, 269, 635
SlaI CTCGAG 1 cut(s) 419
SmiMI CAYNNNNRTG 1 cut(s) 191
SmlI CTYRAG 1 cut(s) 419
SmoI CTYRAG 1 cut(s) 419
SphI GCATGC 1 cut(s) 789
Sse9I AATT 5 cut(s) 273, 558, 640, 673, 756
SspDI GGCGCC 1 cut(s) 437
StyD4I CCNGG 2 cut(s) 104, 305
TaaI ACNGT 1 cut(s) 511
TaiI ACGT 1 cut(s) 225
TaqI TCGA 5 cut(s) 367, 375, 395, 420, 653
TasI AATT 5 cut(s) 273, 558, 640, 673, 756
TfiI GAWTC 2 cut(s) 49, 111
TscAI CASTG 1 cut(s) 540
TseFI GTSAC 2 cut(s) 79, 548
TseI GCWGC 3 cut(s) 689, 692, 749
Tsp45I GTSAC 2 cut(s) 79, 548
TspDTI ATGAA 3 cut(s) 109, 518, 562
TspGWI ACGGA 2 cut(s) 73, 196
TspRI CASTG 1 cut(s) 540
VpaK11BI GGWCC 4 cut(s) 60, 163, 269, 635
XceI RCATGY 1 cut(s) 789
XhoI CTCGAG 1 cut(s) 419
ZraI GACGTC 1 cut(s) 223
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.