pycom05g30970

UDP-3-O-acylglucosamine N-acyltransferase 2

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
pyrus_communis
Chr5
OG0004668
Physical Location & Seq
Forward (+)
30885006 .. 30887209
2204 bp
Loading structure...
UTR
Exon/CDS
Intron
N/A

Sequence Viewer

Length: 867 bp
ATGGCAAGCGCTCTTAGAAGACTTGCAGCTGCATTCTGCTACTCGCCACATTTCCAATTCGTTCGAAGCTTCTCTCTGTTGCGCTTTGGAAATAGTTACCGTTCCTTTGTGTATTCGGCTTCGTCTGAGGAGTGTGCTCCAACTGGTCATCCAGAATTCCGACAATGGAAGAACGGAGGTGGGATTTTCCACAAGTCTGCCTGCATTGACCCAACAGTTTTCATAGAGTTTGGTGCTGTTGTTCATTCTAAATCCGTTGTCTGCGAACATGTTTGTATTGGTTCTGGAGCTGTCGTTGGACCTTCGGTTACAGTTGGGCAATCAACGAAAATTGGCTACAATGTTGCACTCAGTAATTGCAGCATAGGAGATTCGTGCATAATCCACAGTGGCGTATGCATCGGTCAAGATGGATTTGGATTTATGGTGGATGAGCATGGGAATATGTTAAAGAGGCCGCAAATGCTGAATGCTAGGATTGGAAACCACGTGGAGATTGGTGCAAATACTTGCATTGACAGGGGCAGTTGGAGAGATACCGTTTTAGGGGATCATTCAAAGATAGACAATTTAGTTCAGATTGGTCATAATGTAGTTATTGGGAACAACTGCATAATTTGTGGACAAGTTGGGATTGCAGGCTCAGTGACGATAGGAGACTATGTAACTATGGGAGGGAGGGTAGCAGTTCGTGACCATGTATCCATTATATCAAAGGTACGGCTTGCTGGTATGAGCTGTGTCATGAAAGATATTACAGAACCTGGAGATTACGGTGGCTTCCCTGCTGTTCCAGCCCATGAGTGGCGAAGACAAGTTACAAACTACAGTCGGACATCAAAACAGCGGAACCCTGAGAATCATTAG

Protein Analysis

289

Amino Acids

31.11

Weight (kDa)

8.45

Isoelectric Point (pI)

36.65

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
Hexapep PF00132 179 - 214 4.3e-08 Bacterial transferase hexapeptide (six repeats)
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

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Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AciI CCGC 2 cut(s) 458, 847
AclWI GGATC 1 cut(s) 558
AcsI RAATTY 1 cut(s) 155
AcvI CACGTG 1 cut(s) 490
AfaI GTAC 1 cut(s) 720
AfeI AGCGCT 1 cut(s) 10
AfiI CCNNNNNNNGG 2 cut(s) 546, 804
AflIII ACRYGT 1 cut(s) 268
AgsI TTSAA 1 cut(s) 558
AjnI CCWGG 1 cut(s) 763
AluBI AGCT 4 cut(s) 29, 69, 290, 738
AluI AGCT 4 cut(s) 29, 69, 290, 738
Alw21I GWGCWC 1 cut(s) 139
Alw26I GTCTC 1 cut(s) 651
AlwI GGATC 1 cut(s) 558
AlwNI CAGNNNCTG 1 cut(s) 764
Aor51HI AGCGCT 1 cut(s) 10
AoxI GGCC 1 cut(s) 455
ApeKI GCWGC 3 cut(s) 26, 29, 360
ApoI RAATTY 1 cut(s) 155
AspLEI GCGC 2 cut(s) 11, 84
AspS9I GGNCC 1 cut(s) 299
AsuII TTCGAA 1 cut(s) 64
AvaII GGWCC 1 cut(s) 299
BarI GAAGNNNNNNTAC 2 cut(s) 802, 834
BbrPI CACGTG 1 cut(s) 490
BbsI GAAGAC 2 cut(s) 25, 817
Bbv12I GWGCWC 1 cut(s) 139
BbvI GCAGC 3 cut(s) 16, 38, 372
BccI CCATC 1 cut(s) 404
BceAI ACGGC 1 cut(s) 737
BciT130I CCWGG 1 cut(s) 765
BciVI GTATCC 1 cut(s) 712
BcoDI GTCTC 1 cut(s) 651
BfaI CTAG 1 cut(s) 474
BfmI CTRYAG 1 cut(s) 826
BfoI RGCGCY 1 cut(s) 12
BfuI GTATCC 1 cut(s) 712
BisI GCNGC 4 cut(s) 27, 30, 361, 458
BlsI GCNGC 4 cut(s) 28, 31, 362, 459
Bme1390I CCNGG 1 cut(s) 765
Bme18I GGWCC 1 cut(s) 299
BmgT120I GGNCC 1 cut(s) 299
BmiI GGNNCC 1 cut(s) 851
BmrFI CCNGG 1 cut(s) 765
BmsI GCATC 1 cut(s) 408
BpiI GAAGAC 2 cut(s) 25, 817
BpmI CTGGAG 2 cut(s) 306, 786
Bpu14I TTCGAA 1 cut(s) 64
BsaAI YACGTR 1 cut(s) 490
BsaXI ACNNNNNCTCC 6 cut(s) 279, 309, 648, 666, 678, 696
Bsc4I CCNNNNNNNGG 2 cut(s) 546, 804
Bse1I ACTGG 1 cut(s) 148
BseBI CCWGG 1 cut(s) 765
BseGI GGATG 2 cut(s) 148, 436
BseLI CCNNNNNNNGG 2 cut(s) 546, 804
BseMII CTCAG 4 cut(s) 117, 364, 657, 846
BseNI ACTGG 1 cut(s) 148
BseRI GAGGAG 1 cut(s) 143
BseXI GCAGC 3 cut(s) 16, 38, 372
BshFI GGCC 1 cut(s) 457
BsiHKAI GWGCWC 1 cut(s) 139
BslI CCNNNNNNNGG 2 cut(s) 546, 804
BsmAI GTCTC 1 cut(s) 651
BsmI GAATGC 2 cut(s) 32, 475
BsnI GGCC 1 cut(s) 457
Bsp119I TTCGAA 1 cut(s) 64
Bsp1286I GDGCHC 1 cut(s) 139
Bsp143I GATC 1 cut(s) 550
BspACI CCGC 2 cut(s) 458, 847
BspANI GGCC 1 cut(s) 457
BspCNI CTCAG 4 cut(s) 118, 363, 656, 847
BspHI TCATGA 1 cut(s) 744
BspLI GGNNCC 1 cut(s) 851
BspPI GGATC 1 cut(s) 558
BspT104I TTCGAA 1 cut(s) 64
BsrI ACTGG 1 cut(s) 148
BssMI GATC 1 cut(s) 550
Bst2UI CCWGG 1 cut(s) 765
Bst4CI ACNGT 7 cut(s) 101, 217, 313, 389, 541, 776, 830
BstBAI YACGTR 1 cut(s) 490
BstBI TTCGAA 1 cut(s) 64
BstC8I GCNNGC 4 cut(s) 7, 202, 640, 726
BstDEI CTNAG 5 cut(s) 14, 126, 350, 643, 855
BstF5I GGATG 2 cut(s) 148, 436
BstH2I RGCGCY 1 cut(s) 12
BstHHI GCGC 2 cut(s) 11, 84
BstKTI GATC 1 cut(s) 553
BstMAI GTCTC 1 cut(s) 651
BstMBI GATC 1 cut(s) 550
BstMWI GCNNNNNNNGC 2 cut(s) 463, 794
BstNI CCWGG 1 cut(s) 765
BstNSI RCATGY 1 cut(s) 272
BstSCI CCNGG 1 cut(s) 763
BstSFI CTRYAG 1 cut(s) 826
BstV1I GCAGC 3 cut(s) 16, 38, 372
BstV2I GAAGAC 2 cut(s) 25, 817
BsuI GTATCC 1 cut(s) 712
BsuRI GGCC 1 cut(s) 457
BtsCI GGATG 2 cut(s) 148, 436
BtsIMutI CAGTG 2 cut(s) 394, 651
Cac8I GCNNGC 4 cut(s) 7, 202, 640, 726
CaiI CAGNNNCTG 1 cut(s) 764
CciI TCATGA 1 cut(s) 744
CfoI GCGC 2 cut(s) 11, 84
Cfr13I GGNCC 1 cut(s) 299
Csp6I GTAC 1 cut(s) 719
CviAII CATG 5 cut(s) 269, 437, 698, 745, 800
CviQI GTAC 1 cut(s) 719
DdeI CTNAG 5 cut(s) 14, 126, 350, 643, 855
DpnI GATC 1 cut(s) 552
DpnII GATC 1 cut(s) 550
Eco47I GGWCC 1 cut(s) 299
Eco47III AGCGCT 1 cut(s) 10
Eco72I CACGTG 1 cut(s) 490
EcoRI GAATTC 1 cut(s) 155
EcoRII CCWGG 1 cut(s) 763
EcoT22I ATGCAT 1 cut(s) 401
FaeI CATG 5 cut(s) 272, 440, 701, 748, 803
FatI CATG 5 cut(s) 268, 436, 697, 744, 799
Fnu4HI GCNGC 4 cut(s) 27, 30, 361, 458
FokI GGATG 2 cut(s) 135, 443
Fsp4HI GCNGC 4 cut(s) 27, 30, 361, 458
FspBI CTAG 1 cut(s) 474
GlaI GCGC 2 cut(s) 10, 83
GluI GCNGC 4 cut(s) 27, 30, 361, 458
GsuI CTGGAG 2 cut(s) 306, 786
HaeII RGCGCY 1 cut(s) 12
HaeIII GGCC 1 cut(s) 457
HhaI GCGC 2 cut(s) 11, 84
Hin1II CATG 5 cut(s) 272, 440, 701, 748, 803
Hin6I GCGC 2 cut(s) 9, 82
HinP1I GCGC 2 cut(s) 9, 82
HindIII AAGCTT 1 cut(s) 67
HinfI GANTC 2 cut(s) 371, 859
Hpy166II GTNNAC 1 cut(s) 623
Hpy188I TCNGA 4 cut(s) 127, 161, 579, 834
Hpy188III TCNNGA 5 cut(s) 152, 285, 407, 692, 745
Hpy8I GTNNAC 1 cut(s) 623
HpyAV CCTTC 1 cut(s) 312
HpyCH4III ACNGT 7 cut(s) 101, 217, 313, 389, 541, 776, 830
HpyCH4IV ACGT 1 cut(s) 489
HpyF10VI GCNNNNNNNGC 2 cut(s) 463, 794
HpyF3I CTNAG 5 cut(s) 14, 126, 350, 643, 855
HpySE526I ACGT 1 cut(s) 489
Hsp92II CATG 5 cut(s) 272, 440, 701, 748, 803
HspAI GCGC 2 cut(s) 9, 82
Kzo9I GATC 1 cut(s) 550
LmnI GCTCC 2 cut(s) 142, 287
Lsp1109I GCAGC 3 cut(s) 16, 38, 372
LweI GCATC 1 cut(s) 408
MaeI CTAG 1 cut(s) 474
MaeII ACGT 1 cut(s) 489
MaeIII GTNAC 6 cut(s) 95, 307, 646, 664, 692, 817
MalI GATC 1 cut(s) 552
MboI GATC 1 cut(s) 550
MboII GAAGA 3 cut(s) 30, 181, 822
MhlI GDGCHC 1 cut(s) 139
MluCI AATT 6 cut(s) 56, 155, 330, 355, 568, 615
MmeI TCCRAC 5 cut(s) 164, 184, 277, 509, 812
MnlI CCTC 5 cut(s) 121, 170, 447, 668, 672
Mph1103I ATGCAT 1 cut(s) 401
MseI TTAA 1 cut(s) 449
MspA1I CMGCKG 2 cut(s) 29, 847
MspR9I CCNGG 1 cut(s) 765
Mva1269I GAATGC 2 cut(s) 32, 475
MvaI CCWGG 1 cut(s) 765
MwoI GCNNNNNNNGC 2 cut(s) 463, 794
NdeII GATC 1 cut(s) 550
NlaIII CATG 5 cut(s) 272, 440, 701, 748, 803
NlaIV GGNNCC 1 cut(s) 851
NmuCI GTSAC 2 cut(s) 646, 692
NsiI ATGCAT 1 cut(s) 401
NspI RCATGY 1 cut(s) 272
NspV TTCGAA 1 cut(s) 64
PagI TCATGA 1 cut(s) 744
PciI ACATGT 1 cut(s) 268
PctI GAATGC 2 cut(s) 32, 475
PfeI GAWTC 2 cut(s) 371, 859
PkrI GCNGC 4 cut(s) 28, 31, 362, 459
PmaCI CACGTG 1 cut(s) 490
PmlI CACGTG 1 cut(s) 490
Ppu21I YACGTR 1 cut(s) 490
PscI ACATGT 1 cut(s) 268
Psp6I CCWGG 1 cut(s) 763
PspCI CACGTG 1 cut(s) 490
PspGI CCWGG 1 cut(s) 763
PspN4I GGNNCC 1 cut(s) 851
PspPI GGNCC 1 cut(s) 299
PstNI CAGNNNCTG 1 cut(s) 764
PvuII CAGCTG 1 cut(s) 29
RsaI GTAC 1 cut(s) 720
RsaNI GTAC 1 cut(s) 719
SaqAI TTAA 1 cut(s) 449
SatI GCNGC 4 cut(s) 27, 30, 361, 458
Sau3AI GATC 1 cut(s) 550
Sau96I GGNCC 1 cut(s) 299
ScrFI CCNGG 1 cut(s) 765
SduI GDGCHC 1 cut(s) 139
SetI ASST 9 cut(s) 31, 71, 181, 292, 304, 492, 720, 740, 766
SfaNI GCATC 1 cut(s) 408
SfcI CTRYAG 1 cut(s) 826
SfuI TTCGAA 1 cut(s) 64
SinI GGWCC 1 cut(s) 299
Sse9I AATT 6 cut(s) 56, 155, 330, 355, 568, 615
SsiI CCGC 2 cut(s) 458, 847
SspMI CTAG 1 cut(s) 474
StyD4I CCNGG 1 cut(s) 763
TaaI ACNGT 7 cut(s) 101, 217, 313, 389, 541, 776, 830
TaiI ACGT 1 cut(s) 492
TaqI TCGA 1 cut(s) 64
TaqII GACCGA 1 cut(s) 392
TasI AATT 6 cut(s) 56, 155, 330, 355, 568, 615
TauI GCSGC 1 cut(s) 460
TfiI GAWTC 2 cut(s) 371, 859
Tru1I TTAA 1 cut(s) 449
Tru9I TTAA 1 cut(s) 449
TscAI CASTG 2 cut(s) 394, 651
TseFI GTSAC 2 cut(s) 646, 692
TseI GCWGC 3 cut(s) 26, 29, 360
Tsp45I GTSAC 2 cut(s) 646, 692
TspDTI ATGAA 3 cut(s) 211, 233, 761
TspGWI ACGGA 2 cut(s) 189, 244
TspRI CASTG 2 cut(s) 394, 651
VpaK11BI GGWCC 1 cut(s) 299
XapI RAATTY 1 cut(s) 155
XceI RCATGY 1 cut(s) 272
XcmI CCANNNNNNNNNTGG 2 cut(s) 494, 801
XspI CTAG 1 cut(s) 474
Zsp2I ATGCAT 1 cut(s) 401
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.