pycom05g32870

transfer protein

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
pyrus_communis
Chr5
OG0006021
Physical Location & Seq
Reverse (-)
32103397 .. 32105557
2161 bp
Loading structure...
UTR
Exon/CDS
Intron
N/A

Sequence Viewer

Length: 846 bp
ATGTTGGTCGATTGTTTAAACTGGAGGCTACAAAATGATATTGACAATATATTAGCCAAGCCCATAGTTCCAACTGAACTATACAGAGGAGTGCGAGATTCACAGCTCATAGGTCTTTCGGGTTACTCAAGAGAGGGCTTGCCCGTCTTTGCCATTGGTGTTGGGCTAAGTACATTTGACAAAGCATCGGTTCACTACTATGTGCAGTCACACATTCAAATTAATGAATATCGAGATCGTGTAATTTTGCCTTCTGCATCGAAGAAGTATGGTAAGCCTATTATCACTTGCATCAAGGTTTTAGATATGACTGGTTTGAAGCTCTCGGCACTAAGCCAAATAAAGTTACTGACAACCATATCAACCATTGATGATTTGAACTACCCGGAGAAGACAAATACATACTACATTGTAAATGCCCCTTATATATTCTCAGCTTGTTGGAAGGTTGTCAAGCCACTTTTGCAGGAGAGGACAAGGAAGAAAGTGCAAGTGTTGTCTGGTTGTGGTAGGGATGAACTGTTGGAGATAATGGATTATGCATCCCTTCCACATTTCTGTGAAGGAGAAGGTTCAGGATCATCTCGAAATTCACAGAATGGAGCTGACAATTGCTTCTCCTTGGACCACCACTTTCATCAACAACTCTACACCCACATCAGGCAGCAGTCTCTGATGAAGGAATCCGTTCAACCAATCAGAAATGGTTCTTTTCATGTGGATGTACCTGAGGCGGCTGCAGAAAGAACTCATATTGCTGAAACGATTGTATCTGAGTTTAACAAGTTTGGTAATCAGAATGGGCTATCTGAGTCTTTAGATGGCCTAAAAATCAATGGCGACTGA
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
No pathways identified.
Pfam Domains
Protein Families
CRAL_TRIO CRAL_TRIO_N

Protein Analysis

282

Amino Acids

31.54

Weight (kDa)

6.5

Isoelectric Point (pI)

35.72

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
CRAL_TRIO PF00650 34 - 189 7.7e-18 CRAL/TRIO domain
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

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Publication-ready
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Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AciI CCGC 1 cut(s) 734
AclWI GGATC 1 cut(s) 586
AcsI RAATTY 1 cut(s) 589
AfaI GTAC 2 cut(s) 172, 726
AfiI CCNNNNNNNGG 1 cut(s) 660
AgsI TTSAA 4 cut(s) 218, 319, 379, 692
AluBI AGCT 4 cut(s) 106, 322, 437, 605
AluI AGCT 4 cut(s) 106, 322, 437, 605
Alw26I GTCTC 1 cut(s) 675
AlwI GGATC 1 cut(s) 586
AlwNI CAGNNNCTG 1 cut(s) 673
AoxI GGCC 1 cut(s) 823
ApeKI GCWGC 2 cut(s) 664, 737
ApoI RAATTY 1 cut(s) 589
AseI ATTAAT 1 cut(s) 222
Asp700I GAANNNNTTC 2 cut(s) 687, 706
AspS9I GGNCC 1 cut(s) 625
AsuC2I CCSGG 1 cut(s) 386
AvaII GGWCC 1 cut(s) 625
AxyI CCTNAGG 1 cut(s) 729
BaeI ACNNNNGTAYC 2 cut(s) 753, 786
BbsI GAAGAC 1 cut(s) 398
BbvI GCAGC 2 cut(s) 676, 724
BccI CCATC 1 cut(s) 815
BcnI CCSGG 1 cut(s) 386
BcoDI GTCTC 1 cut(s) 675
BfmI CTRYAG 1 cut(s) 738
BisI GCNGC 3 cut(s) 665, 735, 738
BlsI GCNGC 3 cut(s) 666, 736, 739
Bme1390I CCNGG 1 cut(s) 386
Bme18I GGWCC 1 cut(s) 625
BmgT120I GGNCC 1 cut(s) 625
BmrFI CCNGG 1 cut(s) 386
BmsI GCATC 4 cut(s) 194, 266, 300, 551
BpiI GAAGAC 1 cut(s) 398
BpmI CTGGAG 1 cut(s) 43
BpuEI CTTGAG 1 cut(s) 112
BpuMI CCSGG 1 cut(s) 386
BsaJI CCNNGG 1 cut(s) 621
Bsc4I CCNNNNNNNGG 1 cut(s) 660
Bse1I ACTGG 2 cut(s) 26, 316
Bse21I CCTNAGG 1 cut(s) 729
BseDI CCNNGG 1 cut(s) 621
BseGI GGATG 3 cut(s) 520, 542, 727
BseLI CCNNNNNNNGG 1 cut(s) 660
BseMII CTCAG 4 cut(s) 447, 720, 765, 801
BseNI ACTGG 2 cut(s) 26, 316
BseRI GAGGAG 1 cut(s) 102
BseXI GCAGC 2 cut(s) 676, 724
BsgI GTGCAG 1 cut(s) 224
BshFI GGCC 1 cut(s) 825
BsiSI CCGG 1 cut(s) 386
BslI CCNNNNNNNGG 1 cut(s) 660
BsmAI GTCTC 1 cut(s) 675
BsnI GGCC 1 cut(s) 825
Bsp143I GATC 2 cut(s) 235, 578
BspACI CCGC 1 cut(s) 734
BspANI GGCC 1 cut(s) 825
BspCNI CTCAG 4 cut(s) 446, 721, 766, 802
BspMAI CTGCAG 1 cut(s) 742
BspPI GGATC 1 cut(s) 586
BsrI ACTGG 2 cut(s) 26, 316
BssECI CCNNGG 1 cut(s) 621
BssMI GATC 2 cut(s) 235, 578
BssT1I CCWWGG 1 cut(s) 621
Bst4CI ACNGT 1 cut(s) 522
BstC8I GCNNGC 1 cut(s) 140
BstDEI CTNAG 6 cut(s) 167, 332, 433, 729, 774, 810
BstF5I GGATG 3 cut(s) 520, 542, 727
BstKTI GATC 2 cut(s) 238, 581
BstMAI GTCTC 1 cut(s) 675
BstMBI GATC 2 cut(s) 235, 578
BstMWI GCNNNNNNNGC 1 cut(s) 463
BstSCI CCNGG 1 cut(s) 384
BstSFI CTRYAG 1 cut(s) 738
BstV1I GCAGC 2 cut(s) 676, 724
BstV2I GAAGAC 1 cut(s) 398
Bsu36I CCTNAGG 1 cut(s) 729
BsuRI GGCC 1 cut(s) 825
BtsCI GGATG 3 cut(s) 520, 542, 727
Cac8I GCNNGC 1 cut(s) 140
CaiI CAGNNNCTG 1 cut(s) 673
Cfr13I GGNCC 1 cut(s) 625
Csp6I GTAC 2 cut(s) 171, 725
CviAII CATG 1 cut(s) 716
CviQI GTAC 2 cut(s) 171, 725
DdeI CTNAG 6 cut(s) 167, 332, 433, 729, 774, 810
DpnI GATC 2 cut(s) 237, 580
DpnII GATC 2 cut(s) 235, 578
DraI TTTAAA 1 cut(s) 18
Eco130I CCWWGG 1 cut(s) 621
Eco47I GGWCC 1 cut(s) 625
Eco81I CCTNAGG 1 cut(s) 729
EcoT14I CCWWGG 1 cut(s) 621
EcoT22I ATGCAT 1 cut(s) 544
ErhI CCWWGG 1 cut(s) 621
FaeI CATG 1 cut(s) 719
FatI CATG 1 cut(s) 715
Fnu4HI GCNGC 3 cut(s) 665, 735, 738
FokI GGATG 3 cut(s) 527, 529, 734
Fsp4HI GCNGC 3 cut(s) 665, 735, 738
GluI GCNGC 3 cut(s) 665, 735, 738
GsuI CTGGAG 1 cut(s) 43
HaeIII GGCC 1 cut(s) 825
HapII CCGG 1 cut(s) 386
Hin1II CATG 1 cut(s) 719
HinfI GANTC 3 cut(s) 98, 683, 812
HpaII CCGG 1 cut(s) 386
Hpy166II GTNNAC 1 cut(s) 193
Hpy188I TCNGA 5 cut(s) 675, 701, 775, 798, 811
Hpy188III TCNNGA 4 cut(s) 129, 233, 576, 585
Hpy8I GTNNAC 1 cut(s) 193
HpyAV CCTTC 6 cut(s) 261, 439, 557, 557, 563, 673
HpyCH4III ACNGT 1 cut(s) 522
HpyCH4V TGCA 7 cut(s) 205, 257, 291, 466, 490, 542, 740
HpyF10VI GCNNNNNNNGC 1 cut(s) 463
HpyF3I CTNAG 6 cut(s) 167, 332, 433, 729, 774, 810
Hsp92II CATG 1 cut(s) 719
Kzo9I GATC 2 cut(s) 235, 578
LmnI GCTCC 1 cut(s) 602
LpnPI CCDG 8 cut(s) 7, 297, 399, 452, 486, 561, 646, 741
Lsp1109I GCAGC 2 cut(s) 676, 724
LweI GCATC 4 cut(s) 194, 266, 300, 551
MaeIII GTNAC 3 cut(s) 122, 207, 345
MalI GATC 2 cut(s) 237, 580
MboI GATC 2 cut(s) 235, 578
MboII GAAGA 3 cut(s) 274, 403, 493
MfeI CAATTG 1 cut(s) 610
MluCI AATT 4 cut(s) 219, 243, 589, 610
MlyI GAGTC 1 cut(s) 821
MmeI TCCRAC 3 cut(s) 95, 422, 504
MnlI CCTC 5 cut(s) 18, 80, 127, 465, 724
Mph1103I ATGCAT 1 cut(s) 544
MroXI GAANNNNTTC 2 cut(s) 687, 706
MseI TTAA 3 cut(s) 17, 222, 780
MslI CAYNNNNRTG 3 cut(s) 198, 558, 720
MspI CCGG 1 cut(s) 386
MspR9I CCNGG 1 cut(s) 386
MssI GTTTAAAC 1 cut(s) 18
MunI CAATTG 1 cut(s) 610
MwoI GCNNNNNNNGC 1 cut(s) 463
NciI CCSGG 1 cut(s) 386
NdeII GATC 2 cut(s) 235, 578
NlaIII CATG 1 cut(s) 719
NmeAIII GCCGAG 1 cut(s) 305
NmuCI GTSAC 1 cut(s) 207
NsiI ATGCAT 1 cut(s) 544
PdmI GAANNNNTTC 2 cut(s) 687, 706
PfeI GAWTC 2 cut(s) 98, 683
PkrI GCNGC 3 cut(s) 666, 736, 739
PleI GAGTC 1 cut(s) 820
PmeI GTTTAAAC 1 cut(s) 18
PpsI GAGTC 1 cut(s) 820
PshBI ATTAAT 1 cut(s) 222
PspPI GGNCC 1 cut(s) 625
PstI CTGCAG 1 cut(s) 742
PstNI CAGNNNCTG 1 cut(s) 673
RsaI GTAC 2 cut(s) 172, 726
RsaNI GTAC 2 cut(s) 171, 725
RseI CAYNNNNRTG 3 cut(s) 198, 558, 720
SaqAI TTAA 3 cut(s) 17, 222, 780
SatI GCNGC 3 cut(s) 665, 735, 738
Sau3AI GATC 2 cut(s) 235, 578
Sau96I GGNCC 1 cut(s) 625
SchI GAGTC 1 cut(s) 821
ScrFI CCNGG 1 cut(s) 386
SetI ASST 9 cut(s) 108, 115, 300, 324, 439, 450, 574, 607, 730
SfaNI GCATC 4 cut(s) 194, 266, 300, 551
SfcI CTRYAG 1 cut(s) 738
SinI GGWCC 1 cut(s) 625
SmiMI CAYNNNNRTG 3 cut(s) 198, 558, 720
SmlI CTYRAG 1 cut(s) 127
SmoI CTYRAG 1 cut(s) 127
Sse9I AATT 4 cut(s) 219, 243, 589, 610
SsiI CCGC 1 cut(s) 734
StyD4I CCNGG 1 cut(s) 384
StyI CCWWGG 1 cut(s) 621
TaaI ACNGT 1 cut(s) 522
TaqI TCGA 4 cut(s) 9, 232, 260, 586
TasI AATT 4 cut(s) 219, 243, 589, 610
TatI WGTACW 1 cut(s) 170
TauI GCSGC 1 cut(s) 737
TfiI GAWTC 2 cut(s) 98, 683
Tru1I TTAA 3 cut(s) 17, 222, 780
Tru9I TTAA 3 cut(s) 17, 222, 780
TseFI GTSAC 1 cut(s) 207
TseI GCWGC 2 cut(s) 664, 737
Tsp45I GTSAC 1 cut(s) 207
TspDTI ATGAA 5 cut(s) 240, 531, 626, 692, 704
TspGWI ACGGA 1 cut(s) 676
VpaK11BI GGWCC 1 cut(s) 625
VspI ATTAAT 1 cut(s) 222
XapI RAATTY 1 cut(s) 589
XmnI GAANNNNTTC 2 cut(s) 687, 706
Zsp2I ATGCAT 1 cut(s) 544
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.