pycom06g03650

E3 ubiquitin-protein ligase

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
pyrus_communis
Chr6
OG0004388
Physical Location & Seq
Forward (+)
4584507 .. 4585889
1383 bp
Loading structure...
UTR
Exon/CDS
Intron
N/A

Sequence Viewer

Length: 1023 bp
ATGGCAGTTCAAGCTCAATACCCTTCCAATATCCTCTTGTTTAATAGAAACTCACAAGAAGGGCGTGATCATTCATTACAACCACACCCAGGAGGATATCTTGATCAATCCCACATGCTTTTCAACAATGGAGGAGGCAACACCAGTCAGCAGCGCAAAAGAGGAAGAGAAACTTCAGCAGCGACCACTGAAGATATCACTCCTCCAATCACTCCCTTCTCTTTGCAACAACAACAACAATCCCAACCTCCTCAGCTCATCGACCTCTCTCAGCTCCACAACCACCTCCACCTCCAAAATCCCAATGTCGTCTCCACCGGCCTCCGCCTATCCTTCGGAGACCAGCAACAACCACAGCAATTCCAACAGCATCACAATCAACAACACCACCACTCTTCAGCCCTCTTCTCTGCATTAACAGAGGACTTTGCCACCCAAATCAAACACCAGCGCGACGAATTAGACCAATTCCTTCAAGCCCAGGGAGAGCAGTTGCGGCGCACGTTAGCAGAGAAAAGGCAGAGGCACTACCGTGCGCTGCTAAACGCAGCGGAGGAATCAGTAGCTCGTAGACTGAGAGAGAAAGAGGTAGAGGTTCAGAAAGCCACGCGCAGAAATTCCGAGTTGGAAGCAAGGGCAGCCCAACTTAGCGTTGAGGCCCAATTTTGGCAGGCCAAGGCCCGAGCCCAACAGGCCACTGCCGTGTCTCTACAGGCCCAACTACAGCAGGCTATGATCAGCGGAGGGTTTGCGGCGGCCCAGGATAGCAGGAGAGGGGAGGAGGGAGTAGTGTGCGCAGCGGGCGTGGAGGGCCGGGCTGAGGATGCCGAGTCGGCCTACATTGACCCGGAACGGGTGACCGTGTTGGGTCCGAGTTGTAAAGCGTGTCGGAAACGAGTTGCCTCGGTTGTTTTGTTACCGTGCCGGCATTTGAGCCTGTGTACCGAATGTGACCAAGTGGTTCAGGCCTGCCCATTGTGCCTAACCTTGAGAAATTCGAGTGTGGAAGTCTATCTTTCTTAG
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
No pathways identified.
Pfam Domains
Protein Families
Prok-RING_4 zf-C3HC4_3

Protein Analysis

341

Amino Acids

37.96

Weight (kDa)

7.23

Isoelectric Point (pI)

68.21

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
zf-C3HC4_3 PF13920 292 - 328 3.2e-09 Zinc finger, C3HC4 type (RING finger)
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Publication-ready
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Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
Acc16I TGCGCA 1 cut(s) 796
AccI GTMKAC 1 cut(s) 571
AccII CGCG 2 cut(s) 453, 610
AciI CCGC 7 cut(s) 325, 496, 551, 741, 752, 755, 800
AcsI RAATTY 2 cut(s) 616, 994
AcuI CTGAAG 3 cut(s) 159, 210, 381
AfaI GTAC 1 cut(s) 943
AfiI CCNNNNNNNGG 4 cut(s) 89, 666, 820, 853
AgsI TTSAA 3 cut(s) 11, 124, 476
AjnI CCWGG 3 cut(s) 88, 480, 759
AleI CACNNNNGTG 2 cut(s) 531, 701
AluBI AGCT 4 cut(s) 14, 256, 274, 566
AluI AGCT 4 cut(s) 14, 256, 274, 566
Alw26I GTCTC 3 cut(s) 316, 333, 711
Ama87I CYCGRG 1 cut(s) 681
ApeKI GCWGC 6 cut(s) 151, 179, 538, 548, 638, 797
ApoI RAATTY 2 cut(s) 616, 994
AspLEI GCGC 6 cut(s) 156, 453, 501, 538, 612, 797
AspS9I GGNCC 6 cut(s) 658, 679, 715, 757, 811, 869
AsuC2I CCSGG 2 cut(s) 815, 848
AsuHPI GGTGA 1 cut(s) 868
AvaI CYCGRG 1 cut(s) 681
AvaII GGWCC 1 cut(s) 869
BanII GRGCYC 1 cut(s) 688
BbvCI CCTCAGC 2 cut(s) 252, 819
BbvI GCAGC 6 cut(s) 163, 191, 525, 560, 650, 809
BceAI ACGGC 1 cut(s) 686
BciT130I CCWGG 3 cut(s) 90, 482, 761
BclI TGATCA 3 cut(s) 67, 103, 735
BcnI CCSGG 2 cut(s) 815, 848
BcoDI GTCTC 3 cut(s) 316, 333, 711
BfmI CTRYAG 2 cut(s) 710, 722
BglI GCCNNNNNGGC 2 cut(s) 692, 833
BisI GCNGC 9 cut(s) 152, 180, 497, 539, 549, 639, 753, 756, 798
BlsI GCNGC 9 cut(s) 153, 181, 498, 540, 550, 640, 754, 757, 799
Bme1390I CCNGG 5 cut(s) 90, 482, 761, 815, 848
Bme18I GGWCC 1 cut(s) 869
BmeT110I CYCGRG 1 cut(s) 681
BmgT120I GGNCC 6 cut(s) 658, 679, 715, 757, 811, 869
BmiI GGNNCC 1 cut(s) 870
BmrFI CCNGG 5 cut(s) 90, 482, 761, 815, 848
BmsI GCATC 2 cut(s) 379, 814
Bpu10I CCTNAGC 2 cut(s) 252, 819
BpuEI CTTGAG 1 cut(s) 1009
BpuMI CCSGG 2 cut(s) 815, 848
BsaI GGTCTC 1 cut(s) 333
BsaJI CCNNGG 6 cut(s) 88, 480, 481, 675, 759, 903
Bsc4I CCNNNNNNNGG 4 cut(s) 89, 666, 820, 853
Bse118I RCCGGY 2 cut(s) 317, 924
Bse1I ACTGG 1 cut(s) 144
BseBI CCWGG 3 cut(s) 90, 482, 761
BseDI CCNNGG 6 cut(s) 88, 480, 481, 675, 759, 903
BseGI GGATG 1 cut(s) 829
BseLI CCNNNNNNNGG 4 cut(s) 89, 666, 820, 853
BseMII CTCAG 4 cut(s) 266, 284, 566, 810
BseNI ACTGG 1 cut(s) 144
BseRI GAGGAG 4 cut(s) 147, 192, 240, 794
BseXI GCAGC 6 cut(s) 163, 191, 525, 560, 650, 809
Bsh1236I CGCG 2 cut(s) 453, 610
BsiHKCI CYCGRG 1 cut(s) 681
BsiSI CCGG 4 cut(s) 318, 814, 848, 925
BslI CCNNNNNNNGG 4 cut(s) 89, 666, 820, 853
BsmAI GTCTC 3 cut(s) 316, 333, 711
BsmBI CGTCTC 1 cut(s) 316
Bso31I GGTCTC 1 cut(s) 333
BsoBI CYCGRG 1 cut(s) 681
Bsp1286I GDGCHC 1 cut(s) 688
Bsp143I GATC 3 cut(s) 67, 103, 735
BspACI CCGC 7 cut(s) 325, 496, 551, 741, 752, 755, 800
BspCNI CTCAG 4 cut(s) 265, 283, 567, 811
BspFNI CGCG 2 cut(s) 453, 610
BspLI GGNNCC 1 cut(s) 870
BspTNI GGTCTC 1 cut(s) 333
BsrFI RCCGGY 2 cut(s) 317, 924
BsrI ACTGG 1 cut(s) 144
BssAI RCCGGY 2 cut(s) 317, 924
BssECI CCNNGG 6 cut(s) 88, 480, 481, 675, 759, 903
BssMI GATC 3 cut(s) 67, 103, 735
BssT1I CCWWGG 1 cut(s) 675
Bst2UI CCWGG 3 cut(s) 90, 482, 761
Bst4CI ACNGT 3 cut(s) 533, 862, 921
Bst6I CTCTTC 3 cut(s) 160, 400, 410
BstC8I GCNNGC 5 cut(s) 672, 729, 802, 926, 970
BstDEI CTNAG 6 cut(s) 252, 270, 575, 647, 819, 1020
BstEII GGTNACC 1 cut(s) 856
BstF5I GGATG 1 cut(s) 829
BstFNI CGCG 2 cut(s) 453, 610
BstHHI GCGC 6 cut(s) 156, 453, 501, 538, 612, 797
BstKTI GATC 3 cut(s) 70, 106, 738
BstMAI GTCTC 3 cut(s) 316, 333, 711
BstMBI GATC 3 cut(s) 67, 103, 735
BstMWI GCNNNNNNNGC 9 cut(s) 11, 496, 638, 692, 801, 810, 824, 833, 978
BstNI CCWGG 3 cut(s) 90, 482, 761
BstNSI RCATGY 1 cut(s) 118
BstPI GGTNACC 1 cut(s) 856
BstSCI CCNGG 5 cut(s) 88, 480, 759, 813, 846
BstSFI CTRYAG 2 cut(s) 710, 722
BstUI CGCG 2 cut(s) 453, 610
BstV1I GCAGC 6 cut(s) 163, 191, 525, 560, 650, 809
BtsCI GGATG 1 cut(s) 829
BtsI GCAGTG 1 cut(s) 696
BtsIMutI CAGTG 2 cut(s) 186, 696
Cac8I GCNNGC 5 cut(s) 672, 729, 802, 926, 970
CfoI GCGC 6 cut(s) 156, 453, 501, 538, 612, 797
Cfr10I RCCGGY 2 cut(s) 317, 924
Cfr13I GGNCC 6 cut(s) 658, 679, 715, 757, 811, 869
Csp6I GTAC 1 cut(s) 942
CviAII CATG 1 cut(s) 115
CviQI GTAC 1 cut(s) 942
DdeI CTNAG 6 cut(s) 252, 270, 575, 647, 819, 1020
DpnI GATC 3 cut(s) 69, 105, 737
DpnII GATC 3 cut(s) 67, 103, 735
Eam1104I CTCTTC 3 cut(s) 160, 400, 410
EarI CTCTTC 3 cut(s) 160, 400, 410
EciI GGCGGA 1 cut(s) 314
Eco130I CCWWGG 1 cut(s) 675
Eco147I AGGCCT 1 cut(s) 968
Eco24I GRGCYC 1 cut(s) 688
Eco31I GGTCTC 1 cut(s) 333
Eco32I GATATC 2 cut(s) 98, 196
Eco47I GGWCC 1 cut(s) 869
Eco57I CTGAAG 3 cut(s) 159, 210, 381
Eco88I CYCGRG 1 cut(s) 681
Eco91I GGTNACC 1 cut(s) 856
EcoO65I GGTNACC 1 cut(s) 856
EcoRII CCWGG 3 cut(s) 88, 480, 759
EcoRV GATATC 2 cut(s) 98, 196
EcoT14I CCWWGG 1 cut(s) 675
EcoT38I GRGCYC 1 cut(s) 688
ErhI CCWWGG 1 cut(s) 675
Esp3I CGTCTC 1 cut(s) 316
FaeI CATG 1 cut(s) 118
FaiI YATR 2 cut(s) 116, 734
FalI AAGNNNNNCTT 3 cut(s) 157, 189, 999
FatI CATG 1 cut(s) 114
FauI CCCGC 1 cut(s) 793
FbaI TGATCA 3 cut(s) 67, 103, 735
FblI GTMKAC 1 cut(s) 571
Fnu4HI GCNGC 9 cut(s) 152, 180, 497, 539, 549, 639, 753, 756, 798
FokI GGATG 1 cut(s) 836
FriOI GRGCYC 1 cut(s) 688
Fsp4HI GCNGC 9 cut(s) 152, 180, 497, 539, 549, 639, 753, 756, 798
FspI TGCGCA 1 cut(s) 796
GlaI GCGC 6 cut(s) 155, 452, 500, 537, 611, 796
GluI GCNGC 9 cut(s) 152, 180, 497, 539, 549, 639, 753, 756, 798
HapII CCGG 4 cut(s) 318, 814, 848, 925
HhaI GCGC 6 cut(s) 156, 453, 501, 538, 612, 797
Hin1II CATG 1 cut(s) 118
Hin6I GCGC 6 cut(s) 154, 451, 499, 536, 610, 795
HinP1I GCGC 6 cut(s) 154, 451, 499, 536, 610, 795
HinfI GANTC 2 cut(s) 557, 830
HpaII CCGG 4 cut(s) 318, 814, 848, 925
HphI GGTGA 1 cut(s) 868
Hpy166II GTNNAC 2 cut(s) 572, 942
Hpy188I TCNGA 5 cut(s) 338, 600, 622, 873, 891
Hpy188III TCNNGA 1 cut(s) 101
Hpy8I GTNNAC 2 cut(s) 572, 942
Hpy99I CGWCG 1 cut(s) 458
HpyAV CCTTC 5 cut(s) 33, 53, 226, 343, 482
HpyCH4III ACNGT 3 cut(s) 533, 862, 921
HpyCH4IV ACGT 1 cut(s) 503
HpyCH4V TGCA 2 cut(s) 226, 413
HpyF10VI GCNNNNNNNGC 9 cut(s) 11, 496, 638, 692, 801, 810, 824, 833, 978
HpyF3I CTNAG 6 cut(s) 252, 270, 575, 647, 819, 1020
HpySE526I ACGT 1 cut(s) 503
Hsp92II CATG 1 cut(s) 118
HspAI GCGC 6 cut(s) 154, 451, 499, 536, 610, 795
KroI GCCGGC 1 cut(s) 924
KroNI GCCGGC 1 cut(s) 926
Ksp22I TGATCA 3 cut(s) 67, 103, 735
Kzo9I GATC 3 cut(s) 67, 103, 735
LmnI GCTCC 1 cut(s) 279
Lsp1109I GCAGC 6 cut(s) 163, 191, 525, 560, 650, 809
LweI GCATC 2 cut(s) 379, 814
MaeII ACGT 1 cut(s) 503
MaeIII GTNAC 3 cut(s) 856, 915, 950
MalI GATC 3 cut(s) 69, 105, 737
MboI GATC 3 cut(s) 67, 103, 735
MboII GAAGA 4 cut(s) 177, 203, 387, 397
MhlI GDGCHC 1 cut(s) 688
MluCI AATT 6 cut(s) 359, 458, 467, 616, 662, 994
MlyI GAGTC 1 cut(s) 839
MmeI TCCRAC 3 cut(s) 388, 606, 869
MroNI GCCGGC 1 cut(s) 924
MseI TTAA 2 cut(s) 42, 416
MslI CAYNNNNRTG 2 cut(s) 531, 701
MspA1I CMGCKG 3 cut(s) 551, 741, 800
MspI CCGG 4 cut(s) 318, 814, 848, 925
MspR9I CCNGG 5 cut(s) 90, 482, 761, 815, 848
MvaI CCWGG 3 cut(s) 90, 482, 761
MvnI CGCG 2 cut(s) 453, 610
MwoI GCNNNNNNNGC 9 cut(s) 11, 496, 638, 692, 801, 810, 824, 833, 978
NaeI GCCGGC 1 cut(s) 926
NciI CCSGG 2 cut(s) 815, 848
NdeII GATC 3 cut(s) 67, 103, 735
NgoMIV GCCGGC 1 cut(s) 924
NlaIII CATG 1 cut(s) 118
NlaIV GGNNCC 1 cut(s) 870
NmeAIII GCCGAG 1 cut(s) 853
NmuCI GTSAC 2 cut(s) 856, 950
NsbI TGCGCA 1 cut(s) 796
NspI RCATGY 1 cut(s) 118
OliI CACNNNNGTG 2 cut(s) 531, 701
PasI CCCWGGG 1 cut(s) 481
PceI AGGCCT 1 cut(s) 968
PdiI GCCGGC 1 cut(s) 926
PfeI GAWTC 1 cut(s) 557
PkrI GCNGC 9 cut(s) 153, 181, 498, 540, 550, 640, 754, 757, 799
PleI GAGTC 1 cut(s) 838
PpsI GAGTC 1 cut(s) 838
Psp6I CCWGG 3 cut(s) 88, 480, 759
PspEI GGTNACC 1 cut(s) 856
PspGI CCWGG 3 cut(s) 88, 480, 759
PspN4I GGNNCC 1 cut(s) 870
PspPI GGNCC 6 cut(s) 658, 679, 715, 757, 811, 869
RsaI GTAC 1 cut(s) 943
RsaNI GTAC 1 cut(s) 942
RseI CAYNNNNRTG 2 cut(s) 531, 701
SaqAI TTAA 2 cut(s) 42, 416
SatI GCNGC 9 cut(s) 152, 180, 497, 539, 549, 639, 753, 756, 798
Sau3AI GATC 3 cut(s) 67, 103, 735
Sau96I GGNCC 6 cut(s) 658, 679, 715, 757, 811, 869
SchI GAGTC 1 cut(s) 839
ScrFI CCNGG 5 cut(s) 90, 482, 761, 815, 848
SduI GDGCHC 1 cut(s) 688
SfaNI GCATC 2 cut(s) 379, 814
SfcI CTRYAG 2 cut(s) 710, 722
SinI GGWCC 1 cut(s) 869
SmiMI CAYNNNNRTG 2 cut(s) 531, 701
SmlI CTYRAG 1 cut(s) 988
SmoI CTYRAG 1 cut(s) 988
Sse9I AATT 6 cut(s) 359, 458, 467, 616, 662, 994
SseBI AGGCCT 1 cut(s) 968
SsiI CCGC 7 cut(s) 325, 496, 551, 741, 752, 755, 800
StuI AGGCCT 1 cut(s) 968
StyD4I CCNGG 5 cut(s) 88, 480, 759, 813, 846
StyI CCWWGG 1 cut(s) 675
TaaI ACNGT 3 cut(s) 533, 862, 921
TaiI ACGT 1 cut(s) 506
TaqI TCGA 2 cut(s) 261, 998
TasI AATT 6 cut(s) 359, 458, 467, 616, 662, 994
TauI GCSGC 3 cut(s) 499, 755, 758
TfiI GAWTC 1 cut(s) 557
Tru1I TTAA 2 cut(s) 42, 416
Tru9I TTAA 2 cut(s) 42, 416
TscAI CASTG 2 cut(s) 193, 703
TseFI GTSAC 2 cut(s) 856, 950
TseI GCWGC 6 cut(s) 151, 179, 538, 548, 638, 797
Tsp45I GTSAC 2 cut(s) 856, 950
TspDTI ATGAA 1 cut(s) 63
TspRI CASTG 2 cut(s) 193, 703
VpaK11BI GGWCC 1 cut(s) 869
XapI RAATTY 2 cut(s) 616, 994
XceI RCATGY 1 cut(s) 118
XmiI GTMKAC 1 cut(s) 571
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.