pycom06g04780

Agenet domain

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
pyrus_communis
Chr6
OG0002656
Physical Location & Seq
Forward (+)
6380896 .. 6390178
9283 bp
Loading structure...
UTR
Exon/CDS
Intron
N/A

Sequence Viewer

Length: 6498 bp
ATGGATTATGATGACAATGATTTTCAAAGCCAGAATCTTCATTTAGCCGGTGAAGGGAGTACTAATTTTCCTCCAGTTTTACAGCCATATGCTCTTCCCAAGTTTGATTTTGATGATAGCCTTCAAGGGCATTTGAGGTTTGATAGTTTGGTTGAAACTGAGGTTTTTCTGGGTATTGAGAATAACGAAGCTAATCACTGGATTGAGGATTTCTCTCGTGGGAGTAGTGGGATAGAGTTTAGTTCCACTGCAGCAGAATCTTGCTCTATATCAAGGCGCAACAATGTTTGGTCCGAGGCCACTTCTTCAGAATCTGTTGAAATGCTATTAAAATCTGTTGGGCAGGAAGAAATTATTCCCCCTGAGACAATCGTTGAGGAATCAGATGCCTGTAAGGAACTGTGTTGCTTAACTGAGCAGATGGAGCCTAATTTGAATAATGATGATAACATTCTTTCTCAAATGGGGGATGTTACAGATCTAGGGCCTACATTACCACGGGTGGAGATTTCCAAAAACCTTTCTGGCTTAAAGGATGTAGGAGGAGATCAGCTTTGTGTTGATGATTCACAAACTCGTGAAGGTAAAATATTAGTTGATGGAAATTCAAATAACTTGGAACCAAATGATCTCAGTGTAAGTAAAGGGAGTCCTTCTACGGATGGGAAATGCAAAGATGCAAAACAGATGGAATTGGACAATGCGGTTGATGAACCTCTAGATGAAAAAGAAGATTCCTCTGCTTCAGGAATGCAAGTTGATAATTTGATATCTGTGCAGAATATTATTACAAGAAGTGATGAGTTGAGTAAAATAGATGTTCAACATAATTTAAAAGATATCAGTGAAGAGGATCCAGACGGAAATGTCTTGCGCATAGAGGCTCAAGTGAATGAAAAAGTAGTTGAAAAGGCTACCTGTTATCCTGAAAACCCTCATTGTTCATCGTTTGAGGTGGAGTCTGTGGAAGGAGGAATTGCTAATCAGGAAAATGTTGTTAGTGTGGAGGAACCATCGGATATGATACTGCAAGAGGATTCTGACTTACATACCCTGGGCGGATGCAGTGACAGGGAATGCTCTGGTGCTGATGCTGACACCAACAAATATGAAACTGTGGTCTTGTTTAAAGGCATAGATACTGGTCTTGATCAATCAGAACTAAGCACACACAGCTTATCACCTAGGGTGTGTGAAAATGATACTAGCTGCTCAGTTGAGGTTAGCAACAAAAATGCTGAAATTTCTTTAAGTATAGATCCTATGCCAAAAGGGGATTCTGACTTACATGTAGCAGACATATTCAGTGACAGGGAGTGCTCTGGGGTTCCTGCTGAAACCAACAAATGTGAAGATACAGTCTTGCTTAAAGACACAGGTAGTGGTGCTGATATTTTTAAATTAAACACAAATGATTTATCACCAATGGTTACCCGAGGCGATGATAGGGTTGCAGTTGAGGTCACCAACAGCAATGCTGGTATTTCTTCAAGTCCATCAAGTCCAGATCCTAAACCGAAGGTGGATTCTGGATGGAATAGCTCCAAGGAGAATTCTTTAGAAACTGGTTTCCAACCAGATAGTGGGACTTCGATCAGAATGTCCGAGGCTTCTTTGTCAGTCATTGAGGAAAATGAGGTCTCAAAGGATGAAAGTAATGAAAATAGTGAAGTTCATTGTAATTTGACTGCAACATGTTCTTCAGCTGAAGTATTTAGTGAAGTACATGTAACTGGATCTTCTAAAAGTTCTCTTGATGCTTTTGGAATTTCTGGAGAGAAATTGAATGCTGACAGGCATGTATCACTTTCTATTGTTGAGGAGTCTTCTCAGATATGTGATGATAATAAAGTTTACGGAGAAGGTGATGTTGGTGATGGACGGGATAGTAACCTGGATCTCTCTTCCAATGAAAAGGATAGTACACAATTGCTCAATGAGTCTAATAGTAAAAATTTGGAGCTTGGTGGATCTGTTGTTAAGGGATCGGGGTCCTCACCATTGTGTGGAGCTAGCACAGATAAAGAGCTGGTTGTTCCAATATTAACAAATGAAGCTGATGGTAATGAACTGGTGGCCAACATTTTCTTGGAAAATTCGAATTTGGCTTCTTGTGGCACAACGAATGCTGTTCCCTTGTCTTCTGGAAATGGTGTAGCCAGCGATATTGTTAGCCACTCAGAGGTTCAAGCAGCACCTTCTTCTGCTGGGGGTTCATACTGTGACAAAAAGGAAGAAACCGCAAATGAAATGTCCAAGGATGCAAGTTTTTCATTTATAGTGCCACTTCCTTTGGTAGAAACAGGGCCAGTTTCTGTTTCTGAAGTTGAAAAAGGTGTTTCCTGTGATAGTTCTGGACAGTTGTTATGCAAGACGGTTGATCAATCTCTGCCTGTCACAGACAGTTGCAATACAGAGGGCCAAAATGAACCACGAAGTGCAGTTGCTAATGAAGTTAGCAAGGGAAACACAAATGAGAGGGACGTAGCTTCTGTTCAGTGTGAATCTTCTGCAAAAGCGGGTGATGCTACTGAAGCTATTTTCTTTGGAAGACAGGATGGCTCAACCATAAAAGAGAGTTTTGAAAAGGCATCTGCAATTGCAACAGAACTAAGTATGGATTCTGACATGCGGCCTGGGCCTTCAACATTGGTGGAAATGTGTGGTGGTACTGCTAAAAATGCAATGGAAGACACTGACTCTTCTGAAGTATCTCAGGATAAGACTTCTGCGGAGGCTACTATGCCCAGCATTAATTGTGATGCTTCTCCGATTTGTGAAGCTTCTATCTGTTCTGCTCCTTTGCCTGAATCTCATGCTAGGTTGGTTGCACCAGAAAGTGGTGGAAGTTCTGTTAATCCGAATAAAAATGATTGTGGTTCAACTAAGGTTGTTGAAACAAGTGAGCTTTCTGAGATTAAACATGAATCAGGTGATATCAAGGGGCCCAAAAATCTGAATGCCCCAGTTTCTGACATTGTTAGGAATGGGGACAATCATTCTCCTAAATCCTGGAATCCAAAAGAAAATGATGCCTCCAAGGATTGGAGAAATGGCACTTCAGATGTGAGTTCACTTGCAGATTTGCCAAAAGGGGATGCTCCCAACAACTTGCAGCCCGTTCCTACTATTATACCTCCCAGATGTGCAGAGGGGTCTATACAAAATTCTGGTTCAGGCCAGCTGGATGCAAAAATCTCACATGATCTTTCTCATGGAGGTTCACTAGTATTTGATGGAGTTGCACGAGGTGCTTCTAAGGTTACCCCTGAACGGAAAACAAGGCGAGCATCTAGCAAGGCAACGGGTAAACAGAGCGCTAAGAAGGGAAGTGCGCAAGCAAGAACTCCTATGAGACAATTAGAAAGAGGAGACAGATCAAGCAGTGTGTCCCAAAACCAATCTGGAATTTTCCAGCTTGTGCAGCCTAGTGAGACAAAGCCCTATGGACATGTGGATGGTGCTATGAAACCCTTTTCTGTTCATACTACTTCCACATCTAGTTTGCCAGATCTGAATACTTCTGCTCCACCATCTGTAATGTTTCAACAGCCGTTCACTGACTTGCAGCAAGTGCAATTACGTGCTCAAATCTTTGTTTATGGAGCTTTAATTCAAGGAATAGCACCTGAAGAAGCTTATATGGTGTCAGCATTTGGGGGACATGATGGTGGAAGGGGCATGTGGGAGAATTCTTGGCGTGTATCCATAGAGAGGCTACACAGTCAAAAGTCTACTCTGGGTAATCCAGAAACTCCTTTGCAATCACGCTCGGGTTCCAGGGCTCCTGACCAAGTAATTAAAGGTGCATTCCAGAGTAAAAGTATTCCCTCACCTCTTGGTCGACCCAGCACGAAGGGTGCTTCACCAATGGGTGCCCCAATGATACCTATTTCATCACCTCTTTGGAGTATTTCTACCCCTGTTTGTGAGGGCCTGCAATATAATTATCAGCAGGCACTTAATCCATTGCATCCTTTCCAAACACAATCAATAGGGAACGTTGTTGGGGGCCATAATACTACGTGGATGCCTCAGCCCTCCTTTCGGGGTCCCTGGCTTACTTCTCCACAGTCTTCTGCAGCTCAAGCCAGTACTCATTTTTCAGCATTTCCTAGTACAGAAGCAGTGCAGTTGACTCCTGTAAAAGAAACATCTTTACCACAATTGCCTAGTAAGAAGCATGTTTCCTCTGGTCCTTCTACTCAGACTGCAGGCCCAACTAGTGTTTTTGCAGGGGTGTCTCCAGTGTTTGACCCAAAAAAAGTGTCGGCATCACATGGTCAGCATTCTGCTGACCCTAAGCCTAGAAAAAGAAAAAAGACTTCAGCCTCTGAGGATCATGTTCAGGTTACTGTGCAAGCTCGATCTCAACCTGAGTCAGCTCTAACACTTGCTGATAGTAGTAGTCTGTCTACATCTGTTGCCATCTCAACCCCAAGTACCATTGTGTCTAAGACCATGCCAGAGAAATTGATTATGTCCATCTTTCCAACGCCTTCTACTGGTCGCCTCAAAAAAGCAGATCAGGATTTGGAGCAGAGGGAAGCTTTTACAGAGGAGACTCTTAGTAAAGTTAAGGAGGCTACGCAACAGGCAGAAGAAGCTGCTGCTTATGCTGCTGCAGCTGTTAGTCACAGCCAAGAAATATGGAATCAGTTGGATAAGCAAAAAAATTCAAGATTGATATTTGATGGCGAATCTAAGTTGGCATCTGCTGCTGCAGCTGTAGCTGCCGCGGCTGCTGTTGCGAAGGCAGCAGCTGCAGCTGCCAATGTTGCATCGAATGCTGCGTTACAAGCAAAATTGATGGCTGAGGAAGCCTCGGTTTCATATAATGATGGGAATGCGAGCCAAAGTATAAGAATGACTACTCCTGTGCCCATTTTAAGGGGTGAAGATGTAACTAATACTTCGACTTCGATCCTTGTTGCTAGGGAGGCTTCTAGAAAGGGAGTTGAAGTTGCATCTGCAGCCTCAAAGCGAGCTGAAAATATGGATGCCATTGTAAAAGCTGCTGAGTTGGCAGCAGAAGCTGTTTCACAAGCTGGAATAATTGTAGCTATGGGTGATCCTTTGCCATTAAGTCAGTTAGTAGAAGCTGGTCCAGAGGGTTATTGGAAAGTACCTCAAGTTTCTTCAGGTCTGGTTATGAAATCAAATGGCATGTCGAGAGAACAGTCAAATTTGGGTACTGTCGGAGAAGATGTTGATTCTTCTTCTAGGCGTTCTAAAGATAGACAATCAGATAAGCACAAAGCACAGCCAACTGCACATGAGAAGTCACCGATTCTGACAGAGGTAAATAAGGAATCAATGGATGATCAACTGAGGTGGGGAGCAGGAACCACAGGGTTTGATACTGCCAGTGAAAAGGGGTCAAGAGGACCAAAAGGTCGCAATGTTTCTGAAATCAGATCAAAGTCTGCCTTGATTGCTGTCGAGAATGATTTTGAAAAGGAAGCAGGAGCATCTGAAGAAAGCAGCATCAAGGACGGTTCTCTAGTAGAGGTTCTCAAAGATGGGGCTGGATTTGGAGAAGCCTGGTTTACTGCTAAGGTATTGTGTTTGCAGGATCGAAAAGCTAGTGTGTGTTACACTGAGCTTCAGTCAGATGAAGGGAAACTGCAGGAATGGGTGGCACTTGATGGCGAAGAAGATAAGCCACCAAAGATACGAATTGCCCGCCCTGTTACCGCTTTGGGACATGACGGAACAAGGAAAAGGCGCAGAGCAGCAATGGCAGATTATACTTGGTCTGTTGGAGATAAAGTTGATGCATGGATACAGGAGAGCTGGTGGGAAGGAGTTGTCACTGAAAAAAACAAGAAAGATGAAACTATATTAACTGTCCACTTTCCAGCACAAGGGGAAAAGTCAGTTGTTAAAGCTTGGCATCTTCGGCCTTCGCTCATTTGGAAGGATGGCAAATGGGTTGAATGGTTTAGTGTACGAAATGACACCTCCTTCCTTGAGGGTGATATGCCCCAGGAAAAGCGACCCAAATTTGACAGTCCTGCAGTGGAAGGCAAAGGGAATGATAATGCGTCAAAAAGCATTGATGTAAATGATTCAGGGAAGCCTGTAGACCCAAGGTTATTGAATTTATCTGCAAATGAAAAGGTATTTAATATGGGTAAGAATACCAGAACTGAGAATAAGCTTGATGCAACCAGAACAATTCGGACAGGATTGCAGAAGGAAAGATCAAGGGGGGTTGTTTTTGGTATCCCCAAGCCTGGAAAGAAGAGAAAATTTATGGAAGTTAGCAAACATTATGTGGAAAATGAGGGTAGCAAGATTAATGAAACAAGTGATCCGGTGAAAACTGCAAAATACTTGATGCCTCAAGGATCAGGTTCCCGTGGATTGAAAAATACATCCAAAATCGACACGAGGGAAAAACAAGTGGCTGGATCCAAGCTAAAGGGTCTGATCGATCTGGAAAGCTGCAAAGTTTATCCGGTAAATCTGCTGCACAGAAGGACAACCTTTTAA
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
No pathways identified.
Pfam Domains
Protein Families
Agenet

Protein Analysis

2166

Amino Acids

231.7

Weight (kDa)

4.91

Isoelectric Point (pI)

46.21

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
Agenet PF05641 1833 - 1893 6.4e-08 Agenet domain
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AasI GACNNNNNNGTC 2 cut(s) 866, 5394
Acc16I TGCGCA 2 cut(s) 875, 3336
AccB1I GGYRCC 1 cut(s) 3875
AccB7I CCANNNNNTGG 4 cut(s) 1583, 2006, 2840, 3045
AccI GTMKAC 4 cut(s) 3734, 3844, 4415, 6087
AccII CGCG 1 cut(s) 4739
AclI AACGTT 1 cut(s) 4002
AcoI YGGCCR 1 cut(s) 2076
AdeI CACNNNGTG 2 cut(s) 2438, 3251
AfeI AGCGCT 1 cut(s) 3319
AflIII ACRYGT 4 cut(s) 1288, 1694, 1726, 3451
AhlI ACTAGT 2 cut(s) 3226, 4223
AjnI CCWGG 9 cut(s) 1053, 1893, 2635, 3011, 3779, 4055, 5543, 5988, 6238
AjuI GAANNNNNNNTTGG 4 cut(s) 1261, 1293, 1854, 1886
AleI CACNNNNGTG 1 cut(s) 2002
Alw21I GWGCWC 2 cut(s) 1322, 3589
Alw26I GTCTC 7 cut(s) 359, 1645, 3349, 3366, 3428, 4248, 4556
AlwNI CAGNNNCTG 6 cut(s) 314, 2315, 2972, 4334, 4763, 5036
Ama87I CYCGRG 2 cut(s) 1434, 3772
Aor51HI AGCGCT 1 cut(s) 3319
ApaI GGGCCC 1 cut(s) 2949
ArsI GACNNNNNNTTYG 6 cut(s) 2237, 2269, 4253, 4285, 5153, 5185
AseI ATTAAT 2 cut(s) 2754, 6303
Asp700I GAANNNNTTC 1 cut(s) 354
AspA2I CCTAGG 1 cut(s) 1184
AspLEI GCGC 5 cut(s) 279, 876, 3320, 3337, 5730
AsuII TTCGAA 1 cut(s) 2099
AsuNHI GCTAGC 1 cut(s) 2012
AvaI CYCGRG 2 cut(s) 1434, 3772
AvaII GGWCC 6 cut(s) 291, 1992, 4052, 4196, 5105, 5387
AvrII CCTAGG 1 cut(s) 1184
BaeGI GKGCMC 3 cut(s) 2949, 3880, 4884
BalI TGGCCA 1 cut(s) 2078
BamHI GGATCC 2 cut(s) 853, 6416
BanI GGYRCC 1 cut(s) 3875
BanII GRGCYC 2 cut(s) 2949, 3787
BauI CACGAG 4 cut(s) 216, 576, 3246, 6394
BbsI GAAGAC 5 cut(s) 1818, 2133, 2557, 2697, 4068
Bbv12I GWGCWC 2 cut(s) 1322, 3589
BbvCI CCTCAGC 2 cut(s) 4035, 4815
BceAI ACGGC 1 cut(s) 3538
BciT130I CCWGG 9 cut(s) 1055, 1895, 2637, 3013, 3781, 4057, 5545, 5990, 6240
BciVI GTATCC 3 cut(s) 3715, 5778, 6239
BclI TGATCA 3 cut(s) 1150, 2380, 5323
BcoDI GTCTC 7 cut(s) 359, 1645, 3349, 3366, 3428, 4248, 4556
BcuI ACTAGT 2 cut(s) 3226, 4223
BfoI RGCGCY 1 cut(s) 3321
BfuI GTATCC 3 cut(s) 3715, 5778, 6239
BglII AGATCT 2 cut(s) 478, 3511
BlnI CCTAGG 1 cut(s) 1184
BmcAI AGTACT 2 cut(s) 61, 4096
Bme1390I CCNGG 9 cut(s) 1055, 1895, 2637, 3013, 3781, 4057, 5545, 5990, 6240
Bme18I GGWCC 6 cut(s) 291, 1992, 4052, 4196, 5105, 5387
BmeT110I CYCGRG 2 cut(s) 1434, 3772
BmrFI CCNGG 9 cut(s) 1055, 1895, 2637, 3013, 3781, 4057, 5545, 5990, 6240
BmrI ACTGGG 1 cut(s) 2960
BmtI GCTAGC 1 cut(s) 2016
BmuI ACTGGG 1 cut(s) 2960
BpiI GAAGAC 5 cut(s) 1818, 2133, 2557, 2697, 4068
BplI GAGNNNNNCTC 4 cut(s) 197, 229, 4808, 4840
BpmI CTGGAG 3 cut(s) 57, 1794, 4230
Bpu10I CCTNAGC 4 cut(s) 4035, 4302, 4815, 5556
Bpu14I TTCGAA 1 cut(s) 2099
BpuEI CTTGAG 5 cut(s) 870, 4071, 5115, 5993, 6333
Bsa29I ATCGAT 1 cut(s) 6438
BsaAI YACGTR 2 cut(s) 3584, 4026
BsaI GGTCTC 1 cut(s) 1645
BsaWI WCCGGW 2 cut(s) 6319, 6463
Bse118I RCCGGY 1 cut(s) 47
Bse3DI GCAATG 5 cut(s) 1480, 2691, 3968, 5407, 5745
BseBI CCWGG 9 cut(s) 1055, 1895, 2637, 3013, 3781, 4057, 5545, 5990, 6240
BseCI ATCGAT 1 cut(s) 6438
BseMI GCAATG 5 cut(s) 1480, 2691, 3968, 5407, 5745
BseRI GAGGAG 4 cut(s) 558, 1835, 3384, 4574
BseSI GKGCMC 3 cut(s) 2949, 3880, 4884
BseYI CCCAGC 3 cut(s) 2207, 2747, 3848
BsgI GTGCAG 7 cut(s) 797, 2460, 3168, 3443, 4151, 5256, 6461
Bsh1236I CGCG 1 cut(s) 4739
BshNI GGYRCC 1 cut(s) 3875
BshVI ATCGAT 1 cut(s) 6438
BsiHKAI GWGCWC 2 cut(s) 1322, 3589
BsiHKCI CYCGRG 2 cut(s) 1434, 3772
BsiSI CCGG 3 cut(s) 48, 6320, 6464
BslFI GGGAC 7 cut(s) 1600, 2495, 3005, 3376, 3675, 4038, 5718
BsmAI GTCTC 7 cut(s) 359, 1645, 3349, 3366, 3428, 4248, 4556
BsmFI GGGAC 7 cut(s) 1600, 2495, 3005, 3376, 3675, 4038, 5718
BsmI GAATGC 9 cut(s) 756, 1082, 1792, 2131, 2965, 3809, 4288, 4792, 4852
Bso31I GGTCTC 1 cut(s) 1645
BsoBI CYCGRG 2 cut(s) 1434, 3772
Bsp119I TTCGAA 1 cut(s) 2099
Bsp120I GGGCCC 1 cut(s) 2945
Bsp1286I GDGCHC 6 cut(s) 1322, 2949, 3589, 3787, 3880, 4884
BspDI ATCGAT 1 cut(s) 6438
BspFNI CGCG 1 cut(s) 4739
BspMAI CTGCAG 9 cut(s) 253, 4084, 4216, 4627, 4726, 4768, 4975, 5631, 6022
BspOI GCTAGC 1 cut(s) 2016
BspQI GCTCTTC 1 cut(s) 99
BspT104I TTCGAA 1 cut(s) 2099
BspT107I GGYRCC 1 cut(s) 3875
BspTNI GGTCTC 1 cut(s) 1645
BsrDI GCAATG 5 cut(s) 1480, 2691, 3968, 5407, 5745
BsrFI RCCGGY 1 cut(s) 47
BssAI RCCGGY 1 cut(s) 47
BssSI CACGAG 4 cut(s) 216, 576, 3246, 6394
BssT1I CCWWGG 5 cut(s) 1184, 1545, 2256, 3039, 6092
Bst2BI CACGAG 4 cut(s) 216, 576, 3246, 6394
Bst2UI CCWGG 9 cut(s) 1055, 1895, 2637, 3013, 3781, 4057, 5545, 5990, 6240
Bst6I CTCTTC 5 cut(s) 99, 843, 1909, 2707, 6242
BstAPI GCANNNNNTGC 5 cut(s) 3251, 3574, 4718, 4763, 4787
BstBAI YACGTR 2 cut(s) 3584, 4026
BstBI TTCGAA 1 cut(s) 2099
BstDSI CCRYGG 3 cut(s) 497, 4737, 6364
BstEII GGTNACC 3 cut(s) 1429, 1462, 3262
BstFNI CGCG 1 cut(s) 4739
BstH2I RGCGCY 1 cut(s) 3321
BstHHI GCGC 5 cut(s) 279, 876, 3320, 3337, 5730
BstMAI GTCTC 7 cut(s) 359, 1645, 3349, 3366, 3428, 4248, 4556
BstNI CCWGG 9 cut(s) 1055, 1895, 2637, 3013, 3781, 4057, 5545, 5990, 6240
BstNSI RCATGY 9 cut(s) 1292, 1698, 1730, 1802, 2632, 3455, 3685, 4187, 5170
BstPI GGTNACC 3 cut(s) 1429, 1462, 3262
BstSCI CCNGG 9 cut(s) 1053, 1893, 2635, 3011, 3779, 4055, 5543, 5988, 6238
BstSLI GKGCMC 3 cut(s) 2949, 3880, 4884
BstUI CGCG 1 cut(s) 4739
BstV2I GAAGAC 5 cut(s) 1818, 2133, 2557, 2697, 4068
BstX2I RGATCY 9 cut(s) 478, 853, 1258, 1507, 1736, 1897, 1970, 3511, 6416
BstYI RGATCY 9 cut(s) 478, 853, 1258, 1507, 1736, 1897, 1970, 3511, 6416
Bsu15I ATCGAT 1 cut(s) 6438
BsuI GTATCC 3 cut(s) 3715, 5778, 6239
BsuTUI ATCGAT 1 cut(s) 6438
BtgI CCRYGG 3 cut(s) 497, 4737, 6364
BtgZI GCGATG 1 cut(s) 1455
BtsI GCAGTG 5 cut(s) 246, 1072, 3391, 4134, 6027
CaiI CAGNNNCTG 6 cut(s) 314, 2315, 2972, 4334, 4763, 5036
CfoI GCGC 5 cut(s) 279, 876, 3320, 3337, 5730
Cfr10I RCCGGY 1 cut(s) 47
Cfr42I CCGCGG 1 cut(s) 4740
ClaI ATCGAT 1 cut(s) 6438
CseI GACGC 1 cut(s) 6036
CspCI CAANNNNNGTGG 8 cut(s) 2274, 2309, 2634, 2669, 5315, 5338, 5350, 5373
DraI TTTAAA 3 cut(s) 834, 1129, 1399
DraIII CACNNNGTG 2 cut(s) 2438, 3251
DrdI GACNNNNNNGTC 2 cut(s) 866, 5394
DseDI GACNNNNNNGTC 2 cut(s) 866, 5394
EaeI YGGCCR 1 cut(s) 2076
Eam1104I CTCTTC 5 cut(s) 99, 843, 1909, 2707, 6242
EarI CTCTTC 5 cut(s) 99, 843, 1909, 2707, 6242
EciI GGCGGA 1 cut(s) 1074
Eco130I CCWWGG 5 cut(s) 1184, 1545, 2256, 3039, 6092
Eco24I GRGCYC 2 cut(s) 2949, 3787
Eco31I GGTCTC 1 cut(s) 1645
Eco32I GATATC 3 cut(s) 771, 841, 2938
Eco47I GGWCC 6 cut(s) 291, 1992, 4052, 4196, 5105, 5387
Eco47III AGCGCT 1 cut(s) 3319
Eco88I CYCGRG 2 cut(s) 1434, 3772
Eco91I GGTNACC 3 cut(s) 1429, 1462, 3262
EcoO109I RGGNCCY 5 cut(s) 485, 1992, 2945, 3934, 4052
EcoO65I GGTNACC 3 cut(s) 1429, 1462, 3262
EcoRI GAATTC 2 cut(s) 1552, 3691
EcoRII CCWGG 9 cut(s) 1053, 1893, 2635, 3011, 3779, 4055, 5543, 5988, 6238
EcoRV GATATC 3 cut(s) 771, 841, 2938
EcoT14I CCWWGG 5 cut(s) 1184, 1545, 2256, 3039, 6092
EcoT22I ATGCAT 1 cut(s) 5782
EcoT38I GRGCYC 2 cut(s) 2949, 3787
ErhI CCWWGG 5 cut(s) 1184, 1545, 2256, 3039, 6092
FalI AAGNNNNNCTT 2 cut(s) 5414, 5446
FaqI GGGAC 7 cut(s) 1600, 2495, 3005, 3376, 3675, 4038, 5718
FauI CCCGC 2 cut(s) 2512, 5693
FauNDI CATATG 1 cut(s) 88
FbaI TGATCA 3 cut(s) 1150, 2380, 5323
FblI GTMKAC 4 cut(s) 3734, 3844, 4415, 6087
FriOI GRGCYC 2 cut(s) 2949, 3787
FspI TGCGCA 2 cut(s) 875, 3336
GlaI GCGC 5 cut(s) 278, 875, 3319, 3336, 5729
GsaI CCCAGC 3 cut(s) 2211, 2751, 3852
GsuI CTGGAG 3 cut(s) 57, 1794, 4230
HaeII RGCGCY 1 cut(s) 3321
HapII CCGG 3 cut(s) 48, 6320, 6464
HgaI GACGC 1 cut(s) 6036
HhaI GCGC 5 cut(s) 279, 876, 3320, 3337, 5730
Hin6I GCGC 5 cut(s) 277, 874, 3318, 3335, 5728
HinP1I GCGC 5 cut(s) 277, 874, 3318, 3335, 5728
HincII GTYRAC 2 cut(s) 3845, 4137
HindII GTYRAC 2 cut(s) 3845, 4137
HindIII AAGCTT 5 cut(s) 2781, 3636, 4548, 5889, 6161
HpaII CCGG 3 cut(s) 48, 6320, 6464
HpyCH4IV ACGT 4 cut(s) 2484, 3583, 4002, 4025
HpySE526I ACGT 4 cut(s) 2484, 3583, 4002, 4025
HspAI GCGC 5 cut(s) 277, 874, 3318, 3335, 5728
KflI GGGWCCC 1 cut(s) 4052
Ksp22I TGATCA 3 cut(s) 1150, 2380, 5323
KspI CCGCGG 1 cut(s) 4740
LguI GCTCTTC 1 cut(s) 99
MaeII ACGT 4 cut(s) 2484, 3583, 4002, 4025
MfeI CAATTG 3 cut(s) 1928, 2598, 4166
MflI RGATCY 9 cut(s) 478, 853, 1258, 1507, 1736, 1897, 1970, 3511, 6416
MhlI GDGCHC 6 cut(s) 1322, 2949, 3589, 3787, 3880, 4884
MlsI TGGCCA 1 cut(s) 2078
MluNI TGGCCA 1 cut(s) 2078
MlyI GAGTC 8 cut(s) 658, 968, 1832, 1949, 2693, 4132, 4388, 4558
MmeI TCCRAC 5 cut(s) 1597, 4517, 4641, 5179, 5743
Mox20I TGGCCA 1 cut(s) 2078
Mph1103I ATGCAT 1 cut(s) 5782
MroXI GAANNNNTTC 1 cut(s) 354
MscI TGGCCA 1 cut(s) 2078
MslI CAYNNNNRTG 3 cut(s) 2002, 3456, 3669
Msp20I TGGCCA 1 cut(s) 2078
MspA1I CMGCKG 7 cut(s) 1706, 3184, 4628, 4727, 4739, 4763, 4769
MspI CCGG 3 cut(s) 48, 6320, 6464
MspR9I CCNGG 9 cut(s) 1055, 1895, 2637, 3013, 3781, 4057, 5545, 5990, 6240
MunI CAATTG 3 cut(s) 1928, 2598, 4166
Mva1269I GAATGC 9 cut(s) 756, 1082, 1792, 2131, 2965, 3809, 4288, 4792, 4852
MvaI CCWGG 9 cut(s) 1055, 1895, 2637, 3013, 3781, 4057, 5545, 5990, 6240
MvnI CGCG 1 cut(s) 4739
NdeI CATATG 1 cut(s) 88
NheI GCTAGC 1 cut(s) 2012
NmuCI GTSAC 8 cut(s) 1067, 1307, 1462, 2222, 2395, 4634, 5283, 5812
NsbI TGCGCA 2 cut(s) 875, 3336
NsiI ATGCAT 1 cut(s) 5782
NspI RCATGY 9 cut(s) 1292, 1698, 1730, 1802, 2632, 3455, 3685, 4187, 5170
NspV TTCGAA 1 cut(s) 2099
OliI CACNNNNGTG 1 cut(s) 2002
PasI CCCWGGG 1 cut(s) 1054
PciI ACATGT 4 cut(s) 1288, 1694, 1726, 3451
PciSI GCTCTTC 1 cut(s) 99
PcsI WCGNNNNNNNCGW 1 cut(s) 4790
PctI GAATGC 9 cut(s) 756, 1082, 1792, 2131, 2965, 3809, 4288, 4792, 4852
PdmI GAANNNNTTC 1 cut(s) 354
PflMI CCANNNNNTGG 4 cut(s) 1583, 2006, 2840, 3045
PfoI TCCNGGA 1 cut(s) 3011
PleI GAGTC 8 cut(s) 657, 967, 1831, 1948, 2693, 4132, 4387, 4558
PpsI GAGTC 8 cut(s) 657, 967, 1831, 1948, 2693, 4132, 4387, 4558
Ppu21I YACGTR 2 cut(s) 3584, 4026
PpuMI RGGWCCY 2 cut(s) 1992, 4052
PscI ACATGT 4 cut(s) 1288, 1694, 1726, 3451
PshBI ATTAAT 2 cut(s) 2754, 6303
Psp1406I AACGTT 1 cut(s) 4002
Psp5II RGGWCCY 2 cut(s) 1992, 4052
Psp6I CCWGG 9 cut(s) 1053, 1893, 2635, 3011, 3779, 4055, 5543, 5988, 6238
PspEI GGTNACC 3 cut(s) 1429, 1462, 3262
PspFI CCCAGC 3 cut(s) 2207, 2747, 3848
PspGI CCWGG 9 cut(s) 1053, 1893, 2635, 3011, 3779, 4055, 5543, 5988, 6238
PspOMI GGGCCC 1 cut(s) 2945
PspPPI RGGWCCY 2 cut(s) 1992, 4052
PsrI GAACNNNNNNTAC 2 cut(s) 2478, 2510
PstI CTGCAG 9 cut(s) 253, 4084, 4216, 4627, 4726, 4768, 4975, 5631, 6022
PstNI CAGNNNCTG 6 cut(s) 314, 2315, 2972, 4334, 4763, 5036
PsuI RGATCY 9 cut(s) 478, 853, 1258, 1507, 1736, 1897, 1970, 3511, 6416
PvuII CAGCTG 6 cut(s) 1706, 3184, 4628, 4727, 4763, 4769
RseI CAYNNNNRTG 3 cut(s) 2002, 3456, 3669
SacII CCGCGG 1 cut(s) 4740
SalI GTCGAC 1 cut(s) 3843
SapI GCTCTTC 1 cut(s) 99
ScaI AGTACT 2 cut(s) 61, 4096
SchI GAGTC 8 cut(s) 658, 968, 1832, 1949, 2693, 4132, 4388, 4558
ScrFI CCNGG 9 cut(s) 1055, 1895, 2637, 3013, 3781, 4057, 5545, 5990, 6240
SduI GDGCHC 6 cut(s) 1322, 2949, 3589, 3787, 3880, 4884
Sfr303I CCGCGG 1 cut(s) 4740
SfuI TTCGAA 1 cut(s) 2099
SgrBI CCGCGG 1 cut(s) 4740
SinI GGWCC 6 cut(s) 291, 1992, 4052, 4196, 5105, 5387
SmiMI CAYNNNNRTG 3 cut(s) 2002, 3456, 3669
SmlI CTYRAG 5 cut(s) 885, 4086, 5130, 5972, 6348
SmoI CTYRAG 5 cut(s) 885, 4086, 5130, 5972, 6348
SpeI ACTAGT 2 cut(s) 3226, 4223
SspI AATATT 3 cut(s) 591, 784, 2043
StyD4I CCNGG 9 cut(s) 1053, 1893, 2635, 3011, 3779, 4055, 5543, 5988, 6238
StyI CCWWGG 5 cut(s) 1184, 1545, 2256, 3039, 6092
TaiI ACGT 4 cut(s) 2487, 3586, 4005, 4028
TatI WGTACW 5 cut(s) 59, 1723, 1922, 4094, 4118
TauI GCSGC 3 cut(s) 2635, 4739, 4742
TseFI GTSAC 8 cut(s) 1067, 1307, 1462, 2222, 2395, 4634, 5283, 5812
Tsp45I GTSAC 8 cut(s) 1067, 1307, 1462, 2222, 2395, 4634, 5283, 5812
TspGWI ACGGA 5 cut(s) 674, 876, 1872, 3289, 5727
Van91I CCANNNNNTGG 4 cut(s) 1583, 2006, 2840, 3045
VpaK11BI GGWCC 6 cut(s) 291, 1992, 4052, 4196, 5105, 5387
VspI ATTAAT 2 cut(s) 2754, 6303
XbaI TCTAGA 2 cut(s) 718, 4946
XceI RCATGY 9 cut(s) 1292, 1698, 1730, 1802, 2632, 3455, 3685, 4187, 5170
XcmI CCANNNNNNNNNTGG 4 cut(s) 1580, 2086, 3401, 5115
XmaJI CCTAGG 1 cut(s) 1184
XmiI GTMKAC 4 cut(s) 3734, 3844, 4415, 6087
XmnI GAANNNNTTC 1 cut(s) 354
ZrmI AGTACT 2 cut(s) 61, 4096
Zsp2I ATGCAT 1 cut(s) 5782
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.