pycom06g07880

lysine-specific demethylase

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
pyrus_communis
Chr6
OG0004720
Physical Location & Seq
Reverse (-)
12848892 .. 12861492
12601 bp
Loading structure...
UTR
Exon/CDS
Intron
N/A

Sequence Viewer

Length: 4572 bp
ATGGGGAAAGGAAAACCTAGGGCTGTGGAGAAGGGAGTTGTAGGTCAAACTTTGAGTGTTTCATCATCTGGATCATTGAATATTCCATCTGCACCTGTGTATTACCCTACTGAGGATGAATTTAGAGCCCCTTTGGAGTATATTTATAAGATCAGGCCCGAGGCCGAGGCGTATGGGATTTGTAGGATAGTTCCACCGAAGAGCTGGAAACCCCCGTTTGCATTGGATTTGGATTCGTTTACATTTCCCACAAAGACGCAGGCCATTCACCAGCTGCAGGTGCGCCCTGCGTCGTGCGATTCCAAGACTTTTGAGTTGGAGTACAATAGGTTTTTGGAGGACCGTTGCGGGAAGAAGTTGCGGAAGAGGGTGGTTTTCCAAGGGGAGGAGTTGGATTTGTGTAAGTTGTTTAATGCTGTGAAGCGATATGGAGGGTATGATAAGGTTGTGAAGGGGAAGAAGTGGGGGGAGGTTGCTCGTTTTGTGAGGCCGGTGAAAGGGATTTCGGAGTGCTCGAAGCATGTATTGTGTCAACTGTATCGTGACCATTTGTATGATTATGAAAAGTATTATAACAAGCTGAACAGAGAGGGGGCCAGAAGATATAAGAGGGGTATGCCTGTGGAGAAGAGGGGTGAACAGAGTTTTGAATGTTCCAGCTCCAAAAGGAGGCGAATCACTAAGGATGGTGGGAAAGTTAAGGTCAGTAAGGTAGAGAAGGAGGATGAGGAGCATGATCAGATATGTGAACAATGCGTAAGTGGCTTGCACGGTGAAGTGATGCTTTTGTGTGATAGGTGTAATAAGGGGTGGCACATTTATTGTCTGTCTCCTCCATTGAAGCAGGTTCCACCAGGGAATTGGTATTGCTTGGAATGCTTGAATTCCGACAAGGAAAGTTTTGGTTTCGTGCCTGGTAAGCAGTTTTCACTGGAAGTTTTTAGGCGTATGGCTAATCGTGCCAAGAGGAAATGGTTTGGGTCAGGATCTGCATCAAGGGTTCAATTAGAGAAAAAGTTTTGGGAAATTGTGGAGGGTTCAGCCGGTGAGGTGGAAGTTATGTATGGCAGTGACTTGGATACTTCCATGTATGGGAGTGGGTTTCCACGTGTAAATGACCAGAAACCAGAATCAACTGAGGCCAAGATATGGGATGAATACTGTAGGAGCCCATGGAATCTCAATAACTTACCCAAACTGAAAGGATCAGTGCTTAGAGCTGTTCATCATAACATTGCTGGTGTTATGGTTCCTTGGCTGTATGTTGGGATGCTGTTCTCATCTTTTTGCTGGCATTTTGAAGATCATTGTTTTTACTCAATGAATTATCATCACTGGGGAGAGCCAAAATGTTGGTACAGCGTCCCTGGTAGGGAAGCCAGTGCATTTGAGAAGGTGATGCGCAACAGTCTTCCTGATCTTTTTGATGCACAACCTGATTTACTCTTTCAGCTTGTCACCATGTTAAATCCATCTGTTTTGCAAGAGAATGGAGTTCCAGTATATAGTGTGCTACAGGAGCCTGGGAATTTTGTCATCACCTTCCCCAGATCTTACCATGGAGGCTTTAATCTTGGTTTGAATTGTGCAGAAGCTGTCAATTTTGCTCCAGCGGACTGGCTACCTCATGGTGGGTTTGGAGCAGGGCTGTATCAGCTTTATCACAAAACTGCCATTCTGTCTCATGAGGAGCTTGTTTGCGTGCTAGCCAAGCAGAGTGACTGTGACAGCAGAGTGTCAACTTATTTGAAGAAAGAATTGACAAGAATCTACAGCAAGGAAAAAACTTGGAGGGAGAGGCTTTGGAGAAAAGGAATCATCGGATCATCCATTATGTCCTCTCGTAAATGCCCTGAATATGTTGGTACCGAAGAGGACCCTACATGCATTATATGCCAACAGTATCTTTTTCTTTCTGCTGTTGTTTGTTGTTGTAGGCCATCTGCATTTGTATGTCTAGAGCATTCGGAACACCTTTGTGAATGCAAATCCAAGAGACACCGTCTTTTGTATCGTCATTCTCTGGCAGAGTTGCATGAATTGGTGCTTGAAATGGATAAACATTGTTTTGGGGAGACAACACAAGGTAGGACTATAAGAAGGCAAACCTCATTCCCCGATGAACCAACGGCTTTGACAAAAAAGGTAAAAGGGGGCCATGCTACTTTTGCTCAACTTGCAGAACAATGGCTTTTGCGCTCATGCAAGATTTCTCAGAATCCGTTTTCAAGAGATGATTATGTGTCTTTATTAAAAGAAGCTGAACAGTTTCTTTGGGCTGGCTCAGAGATGAATCCTGTGCGGGAAACGATCAAGAATTTGATCAGATTTCAGATATGGGCTGAAGGTGTAAGAGATTGTCTATCTAAACTTGAGACTTGGTCATCTCATCGTGGAAACAATGGAGAGAAAGTTCACTTGGATTATATCAATGAGTTGCTGAGTTTTGATGCGGTGCCCTGTCACGAGACTGGGCATCATATTTTGAAGGGCTACACCGAAAAGGCAAAGGTGTTGATACAGGAAATTGAATCTGCTATGTCATCATGCCCAAAGCTATCAGAGTTGGAATTGTTGTATTCCAGAGCCTGTGAGCTTCCAGTCTGTGTGAATGAAAGTGAAAATTTACTACAAAGAATTTCATCAGCAAAGGTCTTGATGGAAGGCATAAGAAAATGTATCTCAGAGACACGTACTGCTGCAATTGAGATTGATGTTGTTTACAATTTGAAATCAGAGATTTCAGAGCTCCAAGTTGAACTCCCAGACACAGATAGGCTTTCGGATCTGTTAAGGAAGGCTGAATCATGTCATGCTCGATGTGGTGAAATTTTGAAAGGTCCTATTATCCTTGAGGATGTTGAGGTGTTTCTGAAAGAATTGGATGGTTTTACTGTTAACACACCAGAGCTGAAGCTTCTGAGGCAGTACCACACTGATGCTGTTTCTTGGATTTCTCTTCTTAATGCTGTTCTTGTGAACATTCACGAAAGGGAGGATCAAAATGTAGTTAATGAGTTAATGCTCATTTTAACAGATGGAGCATCTTTGAGAATTAAAGTTGATCAGTTGTCTATCGTAGAGTTTGAGTTGAAGAAGGCTCAGTGCAGGGAAAAGGCGCTGAGGATGCGAGATACTAAATTGTCTCTGGACTTCATTCAAGAAGTGATGATGGAGGCAAGAATGTTACGTATTGAGGGGGAGAAGTTATTCGTTGATATGTCTGAAGTGTTTGATGCTGCAATGCTATGGGAGGAAAGAGCGAAACATATCCTTGCACATGAGGCTCAGATATCTGACTTTGAAGATGTTATCAGGAGTTCGGAAGACATACATGTGAATTTATCTTCTCTCCTTGATGTCAAGGATGCACTATCAAAGGCTATGATCTGGTTAAGGAGCTCAAAGCCATTTTTAATGACTTGTTTTCCTTTGGTATCTGTTTCAAATTCTTTGCTGAATGTCGACACCTTGAAGGAATTAGTTTCCGAGTCAAAGCATCCGAAGGTTTCTTTGAAAGAAATAAGGATGCTTGAGACTGTTTTGATGAACTGTAAGGAATGGGAGAATGATGCTTGCTCTCTACTGCAAGATACAAGGTGCCTATTTGATATTAGGATCATTGGTGATGGAACAAGAGATGGTCTTCTTTCCAAGATTGAACATCTAATTGCAAGAATTGAATCCTTGGAAAGCACTGGTTTATCTCTTTCTTTTGACTTTAGCGAGCTTGTGAAACTTAAGGAGGCTTGTTCTGTGCTGCAATGGTGTAAGAAGGCTCTTTCTTTCTGCTCTGGAGTTCCTCCTCTTGAGGATGTTGAAGATTTGGTGAGTGCTGCAGGAAATTTATATGGTACATATGCCCCTAGTGCATTGTGGGGCTTGTTGGTTGAAGGGCTCAAATGGCTTGAGCATGCTACAAAGGTTATTTCTGCACCATGCAATTCTGAAAGACACATGTTAAGTGAAGCTGAAGAAGTACTAGCTAAATCTCAGAGTATTGGTGTCTCCTTCCCATTGATGGTTGGTCAACTTGAATGTGCTATTCAGAAACACAAGTCCTGGCTTGAACAAGTGCATCAACTTTTCAGCCTAAGGCCAGAAGAACGATCTTGGTCTCTAACATTGCAGCTAAAGGAACTTGGAGTTTCTGCTGCTTTCAGTTGCACAGAGCGAGATTTGATTGTATCTGAAGTTGAAAGGGTTGAGAGTTGGAAACGACAGTGCATGGATATTGTCAGGGTAGTAGTTGAAGATGATAATTCATTGCTTGGCGGTTTAGAAAAGATGGGACAGACTCTAGATCGATGTATGCGCATATATGAGAAGCCACATGGCTTGAAAGAGAGTAGTTCCTTTGCTTGCTGCTCCAGTGGTTCTCTGGATCAAGAATTTCTTACTTGTTCTTCATGCAAGGACTGCTACCATTTGGGGTGTCTAGGGTCTTCCATAATTGATGGAAAACATGCAGAATATGTGTGCCGATGTTGCCGACATCTGGTATCTGGAACAACTTCTCAAAATGGACGCAGTTCTCCGGGATATATTTGGAGGGGTGTGGCCAGAACTTAA
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
GO:0000122 GO:0000976 GO:0001046 GO:0001047 GO:0001067 GO:0003674 GO:0003676 GO:0003677 GO:0003682 GO:0003690 GO:0003700 GO:0003712 GO:0003713 GO:0003824 GO:0005488 GO:0005515 GO:0005575 GO:0005622 GO:0005623 GO:0005634 GO:0006325 GO:0006355 GO:0006357 GO:0006464 GO:0006482 GO:0006807 GO:0006996 GO:0007623 GO:0008150 GO:0008152 GO:0008214 GO:0008270 GO:0009889 GO:0009890 GO:0009891 GO:0009892 GO:0009893 GO:0009987 GO:0010468 GO:0010556 GO:0010557 GO:0010558 GO:0010604 GO:0010605 GO:0010628 GO:0010629 GO:0010639 GO:0016043 GO:0016569 GO:0016570 GO:0016577 GO:0019219 GO:0019222 GO:0019538 GO:0031056 GO:0031057 GO:0031063 GO:0031064 GO:0031323 GO:0031324 GO:0031325 GO:0031326 GO:0031327 GO:0031328 GO:0031399 GO:0031400 GO:0031490 GO:0032268 GO:0032269 GO:0032451 GO:0032452 GO:0032453 GO:0032501 GO:0032922 GO:0032991 GO:0032993 GO:0033043 GO:0033044 GO:0034647 GO:0034648 GO:0034720 GO:0034721 GO:0035064 GO:0036211 GO:0042393 GO:0043086 GO:0043167 GO:0043169 GO:0043170 GO:0043226 GO:0043227 GO:0043229 GO:0043231 GO:0043412 GO:0043565 GO:0044092 GO:0044212 GO:0044237 GO:0044238 GO:0044260 GO:0044267 GO:0044424 GO:0044464 GO:0045892 GO:0045893 GO:0045934 GO:0045935 GO:0046872 GO:0046914 GO:0048511 GO:0048518 GO:0048519 GO:0048522 GO:0048523 GO:0050789 GO:0050790 GO:0050794 GO:0051090 GO:0051128 GO:0051129 GO:0051171 GO:0051172 GO:0051173 GO:0051246 GO:0051248 GO:0051252 GO:0051253 GO:0051254 GO:0051276 GO:0051336 GO:0051346 GO:0060255 GO:0065007 GO:0065009 GO:0070076 GO:0070988 GO:0071704 GO:0071840 GO:0080090 GO:0090311 GO:0097159 GO:0140030 GO:0140034 GO:0140096 GO:0140110 GO:1901363 GO:1901564 GO:1901725 GO:1901726 GO:1902275 GO:1902679 GO:1902680 GO:1903506 GO:1903507 GO:1903508 GO:1905268 GO:1990837 GO:2000112 GO:2000113 GO:2001141 GO:2001251
KEGG Pathways
Metabolic & Signaling
No pathways identified.
Pfam Domains
Protein Families
ARID JmjC JmjN PHD PLU-1 zf-C5HC2

Protein Analysis

1524

Amino Acids

173.26

Weight (kDa)

6.42

Isoelectric Point (pI)

47.36

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
JmjN PF02375 32 - 65 1e-14 jmjN domain
ARID PF01388 102 - 188 8.7e-13 ARID/BRIGHT DNA binding domain
PHD PF00628 249 - 293 3.1e-08 PHD-finger
JmjC PF02373 419 - 535 5.2e-43 JmjC domain, hydroxylase
zf-C5HC2 PF02928 629 - 681 3.6e-13 C5HC2 zinc finger
PLU-1 PF08429 951 - 1191 1.1e-17 PLU-1-like protein
PLU-1 PF08429 1150 - 1408 1.1e-14 PLU-1-like protein
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AanI TTATAA 2 cut(s) 147, 573
AarI CACCTGC 1 cut(s) 268
Acc16I TGCGCA 2 cut(s) 1403, 4316
Acc36I ACCTGC 2 cut(s) 268, 835
Acc65I GGTACC 1 cut(s) 1865
AccB1I GGYRCC 3 cut(s) 1865, 2455, 3600
AccB7I CCANNNNNTGG 1 cut(s) 1149
AccI GTMKAC 1 cut(s) 3465
AciI CCGC 6 cut(s) 348, 361, 1613, 2302, 2453, 4275
AclWI GGATC 8 cut(s) 79, 994, 1213, 1831, 2793, 3006, 3626, 4392
AcoI YGGCCR 1 cut(s) 4560
AcuI CTGAAG 5 cut(s) 2364, 2933, 3246, 3993, 4212
AcvI CACGTG 1 cut(s) 1109
AfaI GTAC 7 cut(s) 323, 1358, 1867, 2695, 2930, 3856, 3981
AflII CTTAAG 1 cut(s) 3740
AflIII ACRYGT 4 cut(s) 1108, 2690, 3334, 3957
AjnI CCWGG 5 cut(s) 853, 913, 1366, 1522, 4061
AjuI GAANNNNNNNTTGG 2 cut(s) 2402, 2434
AleI CACNNNNGTG 1 cut(s) 1977
AloI GAACNNNNNNTCC 2 cut(s) 2397, 2429
Alw21I GWGCWC 3 cut(s) 515, 2751, 3404
AlwI GGATC 8 cut(s) 79, 994, 1213, 1831, 2793, 3006, 3626, 4392
AlwNI CAGNNNCTG 3 cut(s) 989, 1595, 2589
Ama87I CYCGRG 1 cut(s) 158
ApeKI GCWGC 8 cut(s) 274, 2699, 3239, 3760, 3836, 4129, 4154, 4365
Asp700I GAANNNNTTC 3 cut(s) 2268, 3209, 4513
Asp718I GGTACC 1 cut(s) 1865
AspA2I CCTAGG 1 cut(s) 17
AspLEI GCGC 5 cut(s) 285, 1404, 2199, 3121, 4317
AspS9I GGNCC 6 cut(s) 156, 340, 594, 1876, 2155, 2840
AsuC2I CCSGG 1 cut(s) 4539
AsuNHI GCTAGC 1 cut(s) 1705
AvaI CYCGRG 1 cut(s) 158
AvaII GGWCC 3 cut(s) 340, 1876, 2840
AvrII CCTAGG 1 cut(s) 17
AxyI CCTNAGG 1 cut(s) 4094
BaeGI GKGCMC 1 cut(s) 2460
BalI TGGCCA 1 cut(s) 4562
BanI GGYRCC 3 cut(s) 1865, 2455, 3600
BanII GRGCYC 5 cut(s) 130, 1172, 2751, 3404, 3900
BauI CACGAG 1 cut(s) 2465
BbrPI CACGTG 1 cut(s) 1109
BbsI GAAGAC 4 cut(s) 1403, 3333, 3638, 4437
Bbv12I GWGCWC 3 cut(s) 515, 2751, 3404
BbvCI CCTCAGC 1 cut(s) 3122
BbvI GCAGC 8 cut(s) 261, 2686, 3226, 3747, 3823, 4141, 4141, 4352
BceAI ACGGC 1 cut(s) 2145
BciT130I CCWGG 5 cut(s) 855, 915, 1368, 1524, 4063
BciVI GTATCC 1 cut(s) 1072
BclI TGATCA 3 cut(s) 736, 2322, 3064
BcnI CCSGG 1 cut(s) 4539
BfaI CTAG 7 cut(s) 18, 1706, 1958, 3867, 3983, 4301, 4439
BfmI CTRYAG 5 cut(s) 275, 1162, 1514, 1771, 3837
BfoI RGCGCY 1 cut(s) 3122
BfrI CTTAAG 1 cut(s) 3740
BfuAI ACCTGC 2 cut(s) 268, 835
BfuI GTATCC 1 cut(s) 1072
BglII AGATCT 1 cut(s) 1550
BisI GCNGC 8 cut(s) 275, 2700, 3240, 3761, 3837, 4130, 4155, 4366
BlnI CCTAGG 1 cut(s) 17
BlsI GCNGC 8 cut(s) 276, 2701, 3241, 3762, 3838, 4131, 4156, 4367
BmcAI AGTACT 1 cut(s) 3981
Bme1390I CCNGG 6 cut(s) 855, 915, 1368, 1524, 4063, 4539
Bme18I GGWCC 3 cut(s) 340, 1876, 2840
BmeT110I CYCGRG 1 cut(s) 158
BmgT120I GGNCC 6 cut(s) 156, 340, 594, 1876, 2155, 2840
BmrFI CCNGG 6 cut(s) 855, 915, 1368, 1524, 4063, 4539
BmrI ACTGGG 2 cut(s) 1345, 2481
BmtI GCTAGC 1 cut(s) 1709
BmuI ACTGGG 2 cut(s) 1345, 2481
BpiI GAAGAC 4 cut(s) 1403, 3333, 3638, 4437
BpmI CTGGAG 3 cut(s) 1593, 3816, 4354
Bpu10I CCTNAGC 1 cut(s) 3122
BpuEI CTTGAG 5 cut(s) 2393, 2873, 3554, 3830, 3929
BpuMI CCSGG 1 cut(s) 4539
Bsa29I ATCGAT 1 cut(s) 4306
BsaAI YACGTR 3 cut(s) 1109, 2693, 3191
BsaBI GATNNNNATC 1 cut(s) 991
BsaI GGTCTC 1 cut(s) 4122
BsaXI ACNNNNNCTCC 4 cut(s) 1485, 1515, 2397, 2427
Bse118I RCCGGY 2 cut(s) 490, 1043
Bse1I ACTGG 9 cut(s) 936, 1340, 1380, 1499, 1622, 2476, 2600, 3703, 4371
Bse21I CCTNAGG 1 cut(s) 4094
Bse3DI GCAATG 5 cut(s) 1233, 3249, 3770, 4124, 4265
Bse8I GATNNNNATC 1 cut(s) 991
BseBI CCWGG 5 cut(s) 855, 915, 1368, 1524, 4063
BseCI ATCGAT 1 cut(s) 4306
BseJI GATNNNNATC 1 cut(s) 991
BseMI GCAATG 5 cut(s) 1233, 3249, 3770, 4124, 4265
BseNI ACTGG 9 cut(s) 936, 1340, 1380, 1499, 1622, 2476, 2600, 3703, 4371
BseRI GAGGAG 5 cut(s) 401, 743, 822, 1703, 3795
BseSI GKGCMC 1 cut(s) 2460
BseXI GCAGC 8 cut(s) 261, 2686, 3226, 3747, 3823, 4141, 4141, 4352
BsgI GTGCAG 4 cut(s) 75, 1608, 3127, 3918
BshNI GGYRCC 3 cut(s) 1865, 2455, 3600
BshVI ATCGAT 1 cut(s) 4306
BsiHKAI GWGCWC 3 cut(s) 515, 2751, 3404
BsiHKCI CYCGRG 1 cut(s) 158
BsiSI CCGG 3 cut(s) 491, 1044, 4538
BslFI GGGAC 2 cut(s) 1349, 4305
BsmFI GGGAC 2 cut(s) 1349, 4305
BsmI GAATGC 3 cut(s) 881, 1963, 1988
Bso31I GGTCTC 1 cut(s) 4122
BsoBI CYCGRG 1 cut(s) 158
Bsp1286I GDGCHC 7 cut(s) 130, 515, 1172, 2460, 2751, 3404, 3900
Bsp19I CCATGG 2 cut(s) 1172, 1558
BspACI CCGC 6 cut(s) 348, 361, 1613, 2302, 2453, 4275
BspDI ATCGAT 1 cut(s) 4306
BspHI TCATGA 1 cut(s) 1684
BspMAI CTGCAG 2 cut(s) 279, 3841
BspMI ACCTGC 2 cut(s) 268, 835
BspOI GCTAGC 1 cut(s) 1709
BspPI GGATC 8 cut(s) 79, 994, 1213, 1831, 2793, 3006, 3626, 4392
BspQI GCTCTTC 1 cut(s) 194
BspT107I GGYRCC 3 cut(s) 1865, 2455, 3600
BspTI CTTAAG 1 cut(s) 3740
BspTNI GGTCTC 1 cut(s) 4122
BsrDI GCAATG 5 cut(s) 1233, 3249, 3770, 4124, 4265
BsrFI RCCGGY 2 cut(s) 490, 1043
BsrI ACTGG 9 cut(s) 936, 1340, 1380, 1499, 1622, 2476, 2600, 3703, 4371
BssAI RCCGGY 2 cut(s) 490, 1043
BssSI CACGAG 1 cut(s) 2465
BssT1I CCWWGG 6 cut(s) 17, 379, 1172, 1253, 1558, 3687
Bst2BI CACGAG 1 cut(s) 2465
Bst2UI CCWGG 5 cut(s) 855, 915, 1368, 1524, 4063
Bst6I CTCTTC 5 cut(s) 194, 359, 623, 1866, 2964
BstAFI CTTAAG 1 cut(s) 3740
BstAPI GCANNNNNTGC 2 cut(s) 1893, 4419
BstBAI YACGTR 3 cut(s) 1109, 2693, 3191
BstDSI CCRYGG 2 cut(s) 1172, 1558
BstH2I RGCGCY 1 cut(s) 3122
BstHHI GCGC 5 cut(s) 285, 1404, 2199, 3121, 4317
BstNI CCWGG 5 cut(s) 855, 915, 1368, 1524, 4063
BstNSI RCATGY 6 cut(s) 524, 1887, 3338, 3917, 3961, 4469
BstSCI CCNGG 6 cut(s) 853, 913, 1366, 1522, 4061, 4537
BstSFI CTRYAG 5 cut(s) 275, 1162, 1514, 1771, 3837
BstSLI GKGCMC 1 cut(s) 2460
BstSNI TACGTA 1 cut(s) 3191
BstV1I GCAGC 8 cut(s) 261, 2686, 3226, 3747, 3823, 4141, 4141, 4352
BstV2I GAAGAC 4 cut(s) 1403, 3333, 3638, 4437
BstX2I RGATCY 3 cut(s) 986, 1550, 2785
BstXI CCANNNNNNTGG 3 cut(s) 861, 1353, 1617
BstYI RGATCY 3 cut(s) 986, 1550, 2785
Bsu15I ATCGAT 1 cut(s) 4306
Bsu36I CCTNAGG 1 cut(s) 4094
BsuI GTATCC 1 cut(s) 1072
BsuTUI ATCGAT 1 cut(s) 4306
BtgI CCRYGG 2 cut(s) 1172, 1558
BtsI GCAGTG 1 cut(s) 1075
BveI ACCTGC 2 cut(s) 268, 835
CaiI CAGNNNCTG 3 cut(s) 989, 1595, 2589
CciI TCATGA 1 cut(s) 1684
CfoI GCGC 5 cut(s) 285, 1404, 2199, 3121, 4317
Cfr10I RCCGGY 2 cut(s) 490, 1043
Cfr13I GGNCC 6 cut(s) 156, 340, 594, 1876, 2155, 2840
ClaI ATCGAT 1 cut(s) 4306
CseI GACGC 4 cut(s) 265, 279, 1351, 4536
Csp6I GTAC 7 cut(s) 322, 1357, 1866, 2694, 2929, 3855, 3980
CspCI CAANNNNNGTGG 2 cut(s) 4320, 4355
CviQI GTAC 7 cut(s) 322, 1357, 1866, 2694, 2929, 3855, 3980
EaeI YGGCCR 1 cut(s) 4560
Eam1104I CTCTTC 5 cut(s) 194, 359, 623, 1866, 2964
EarI CTCTTC 5 cut(s) 194, 359, 623, 1866, 2964
Ecl136II GAGCTC 2 cut(s) 2749, 3402
Eco105I TACGTA 1 cut(s) 3191
Eco130I CCWWGG 6 cut(s) 17, 379, 1172, 1253, 1558, 3687
Eco24I GRGCYC 5 cut(s) 130, 1172, 2751, 3404, 3900
Eco31I GGTCTC 1 cut(s) 4122
Eco32I GATATC 1 cut(s) 3294
Eco47I GGWCC 3 cut(s) 340, 1876, 2840
Eco53kI GAGCTC 2 cut(s) 2749, 3402
Eco57I CTGAAG 5 cut(s) 2364, 2933, 3246, 3993, 4212
Eco72I CACGTG 1 cut(s) 1109
Eco81I CCTNAGG 1 cut(s) 4094
Eco88I CYCGRG 1 cut(s) 158
EcoICRI GAGCTC 2 cut(s) 2749, 3402
EcoO109I RGGNCCY 2 cut(s) 1876, 2840
EcoRI GAATTC 1 cut(s) 883
EcoRII CCWGG 5 cut(s) 853, 913, 1366, 1522, 4061
EcoRV GATATC 1 cut(s) 3294
EcoT14I CCWWGG 6 cut(s) 17, 379, 1172, 1253, 1558, 3687
EcoT22I ATGCAT 1 cut(s) 1889
EcoT38I GRGCYC 5 cut(s) 130, 1172, 2751, 3404, 3900
ErhI CCWWGG 6 cut(s) 17, 379, 1172, 1253, 1558, 3687
FalI AAGNNNNNCTT 4 cut(s) 768, 800, 4380, 4412
FaqI GGGAC 2 cut(s) 1349, 4305
FauI CCCGC 2 cut(s) 341, 2295
FauNDI CATATG 1 cut(s) 3859
FbaI TGATCA 3 cut(s) 736, 2322, 3064
FblI GTMKAC 1 cut(s) 3465
Fnu4HI GCNGC 8 cut(s) 275, 2700, 3240, 3761, 3837, 4130, 4155, 4366
FriOI GRGCYC 5 cut(s) 130, 1172, 2751, 3404, 3900
Fsp4HI GCNGC 8 cut(s) 275, 2700, 3240, 3761, 3837, 4130, 4155, 4366
FspAI RTGCGCAY 1 cut(s) 4316
FspBI CTAG 7 cut(s) 18, 1706, 1958, 3867, 3983, 4301, 4439
FspI TGCGCA 2 cut(s) 1403, 4316
GlaI GCGC 5 cut(s) 284, 1403, 2198, 3120, 4316
GluI GCNGC 8 cut(s) 275, 2700, 3240, 3761, 3837, 4130, 4155, 4366
GsuI CTGGAG 3 cut(s) 1593, 3816, 4354
HaeII RGCGCY 1 cut(s) 3122
HapII CCGG 3 cut(s) 491, 1044, 4538
HgaI GACGC 4 cut(s) 265, 279, 1351, 4536
HhaI GCGC 5 cut(s) 285, 1404, 2199, 3121, 4317
Hin6I GCGC 5 cut(s) 283, 1402, 2197, 3119, 4315
HinP1I GCGC 5 cut(s) 283, 1402, 2197, 3119, 4315
HincII GTYRAC 5 cut(s) 533, 1740, 2899, 3466, 4031
HindII GTYRAC 5 cut(s) 533, 1740, 2899, 3466, 4031
HindIII AAGCTT 1 cut(s) 2915
HpaI GTTAAC 1 cut(s) 2899
HpaII CCGG 3 cut(s) 491, 1044, 4538
Hpy99I CGWCG 1 cut(s) 295
HpyCH4IV ACGT 3 cut(s) 1108, 2692, 3190
HpySE526I ACGT 3 cut(s) 1108, 2692, 3190
HspAI GCGC 5 cut(s) 283, 1402, 2197, 3119, 4315
KpnI GGTACC 1 cut(s) 1869
Ksp22I TGATCA 3 cut(s) 736, 2322, 3064
KspAI GTTAAC 1 cut(s) 2899
LguI GCTCTTC 1 cut(s) 194
Lsp1109I GCAGC 8 cut(s) 261, 2686, 3226, 3747, 3823, 4141, 4141, 4352
MaeI CTAG 7 cut(s) 18, 1706, 1958, 3867, 3983, 4301, 4439
MaeII ACGT 3 cut(s) 1108, 2692, 3190
MaeIII GTNAC 7 cut(s) 542, 1070, 1456, 1718, 1724, 2462, 3186
MfeI CAATTG 1 cut(s) 2703
MflI RGATCY 3 cut(s) 986, 1550, 2785
MhlI GDGCHC 7 cut(s) 130, 515, 1172, 2460, 2751, 3404, 3900
MlsI TGGCCA 1 cut(s) 4562
MluNI TGGCCA 1 cut(s) 4562
MlyI GAGTC 2 cut(s) 3500, 4291
MmeI TCCRAC 5 cut(s) 297, 372, 912, 2547, 4193
Mox20I TGGCCA 1 cut(s) 4562
Mph1103I ATGCAT 1 cut(s) 1889
MroXI GAANNNNTTC 3 cut(s) 2268, 3209, 4513
MscI TGGCCA 1 cut(s) 4562
MslI CAYNNNNRTG 5 cut(s) 552, 1951, 1977, 2937, 3335
Msp20I TGGCCA 1 cut(s) 4562
MspA1I CMGCKG 2 cut(s) 274, 1613
MspCI CTTAAG 1 cut(s) 3740
MspI CCGG 3 cut(s) 491, 1044, 4538
MspR9I CCNGG 6 cut(s) 855, 915, 1368, 1524, 4063, 4539
MunI CAATTG 1 cut(s) 2703
Mva1269I GAATGC 3 cut(s) 881, 1963, 1988
MvaI CCWGG 5 cut(s) 855, 915, 1368, 1524, 4063
NciI CCSGG 1 cut(s) 4539
NcoI CCATGG 2 cut(s) 1172, 1558
NdeI CATATG 1 cut(s) 3859
NheI GCTAGC 1 cut(s) 1705
NmeAIII GCCGAG 1 cut(s) 190
NmuCI GTSAC 6 cut(s) 542, 1070, 1456, 1718, 1724, 2462
NsbI TGCGCA 2 cut(s) 1403, 4316
NsiI ATGCAT 1 cut(s) 1889
NspI RCATGY 6 cut(s) 524, 1887, 3338, 3917, 3961, 4469
OliI CACNNNNGTG 1 cut(s) 1977
PaeI GCATGC 1 cut(s) 3917
PagI TCATGA 1 cut(s) 1684
PaqCI CACCTGC 1 cut(s) 268
PciI ACATGT 2 cut(s) 3334, 3957
PciSI GCTCTTC 1 cut(s) 194
PcsI WCGNNNNNNNCGW 1 cut(s) 512
PctI GAATGC 3 cut(s) 881, 1963, 1988
PdmI GAANNNNTTC 3 cut(s) 2268, 3209, 4513
PflFI GACNNNGTC 2 cut(s) 2001, 2380
PflMI CCANNNNNTGG 1 cut(s) 1149
PfoI TCCNGGA 1 cut(s) 4537
PkrI GCNGC 8 cut(s) 276, 2701, 3241, 3762, 3838, 4131, 4156, 4367
PleI GAGTC 2 cut(s) 3499, 4291
PmaCI CACGTG 1 cut(s) 1109
PmlI CACGTG 1 cut(s) 1109
PpsI GAGTC 2 cut(s) 3499, 4291
Ppu21I YACGTR 3 cut(s) 1109, 2693, 3191
PpuMI RGGWCCY 2 cut(s) 1876, 2840
PscI ACATGT 2 cut(s) 3334, 3957
PsiI TTATAA 2 cut(s) 147, 573
Psp124BI GAGCTC 2 cut(s) 2751, 3404
Psp5II RGGWCCY 2 cut(s) 1876, 2840
Psp6I CCWGG 5 cut(s) 853, 913, 1366, 1522, 4061
PspCI CACGTG 1 cut(s) 1109
PspGI CCWGG 5 cut(s) 853, 913, 1366, 1522, 4061
PspPI GGNCC 6 cut(s) 156, 340, 594, 1876, 2155, 2840
PspPPI RGGWCCY 2 cut(s) 1876, 2840
PstI CTGCAG 2 cut(s) 279, 3841
PstNI CAGNNNCTG 3 cut(s) 989, 1595, 2589
PsuI RGATCY 3 cut(s) 986, 1550, 2785
PsyI GACNNNGTC 2 cut(s) 2001, 2380
PvuII CAGCTG 1 cut(s) 274
RsaI GTAC 7 cut(s) 323, 1358, 1867, 2695, 2930, 3856, 3981
RsaNI GTAC 7 cut(s) 322, 1357, 1866, 2694, 2929, 3855, 3980
RseI CAYNNNNRTG 5 cut(s) 552, 1951, 1977, 2937, 3335
SacI GAGCTC 2 cut(s) 2751, 3404
SalI GTCGAC 1 cut(s) 3464
SapI GCTCTTC 1 cut(s) 194
SatI GCNGC 8 cut(s) 275, 2700, 3240, 3761, 3837, 4130, 4155, 4366
Sau96I GGNCC 6 cut(s) 156, 340, 594, 1876, 2155, 2840
ScaI AGTACT 1 cut(s) 3981
SchI GAGTC 2 cut(s) 3500, 4291
ScrFI CCNGG 6 cut(s) 855, 915, 1368, 1524, 4063, 4539
SduI GDGCHC 7 cut(s) 130, 515, 1172, 2460, 2751, 3404, 3900
SfcI CTRYAG 5 cut(s) 275, 1162, 1514, 1771, 3837
SinI GGWCC 3 cut(s) 340, 1876, 2840
SmiMI CAYNNNNRTG 5 cut(s) 552, 1951, 1977, 2937, 3335
SmlI CTYRAG 6 cut(s) 2372, 2852, 3533, 3740, 3809, 3908
SmoI CTYRAG 6 cut(s) 2372, 2852, 3533, 3740, 3809, 3908
SnaBI TACGTA 1 cut(s) 3191
SphI GCATGC 1 cut(s) 3917
SsiI CCGC 6 cut(s) 348, 361, 1613, 2302, 2453, 4275
SspI AATATT 1 cut(s) 82
SspMI CTAG 7 cut(s) 18, 1706, 1958, 3867, 3983, 4301, 4439
SstI GAGCTC 2 cut(s) 2751, 3404
StyD4I CCNGG 6 cut(s) 853, 913, 1366, 1522, 4061, 4537
StyI CCWWGG 6 cut(s) 17, 379, 1172, 1253, 1558, 3687
TaiI ACGT 3 cut(s) 1111, 2695, 3193
TaqI TCGA 4 cut(s) 515, 2818, 3465, 4306
TatI WGTACW 2 cut(s) 321, 3979
TseFI GTSAC 6 cut(s) 542, 1070, 1456, 1718, 1724, 2462
TseI GCWGC 8 cut(s) 274, 2699, 3239, 3760, 3836, 4129, 4154, 4365
Tsp45I GTSAC 6 cut(s) 542, 1070, 1456, 1718, 1724, 2462
TspGWI ACGGA 1 cut(s) 2211
Tth111I GACNNNGTC 2 cut(s) 2001, 2380
Van91I CCANNNNNTGG 1 cut(s) 1149
Vha464I CTTAAG 1 cut(s) 3740
VpaK11BI GGWCC 3 cut(s) 340, 1876, 2840
XbaI TCTAGA 2 cut(s) 1957, 4300
XceI RCATGY 6 cut(s) 524, 1887, 3338, 3917, 3961, 4469
XcmI CCANNNNNNNNNTGG 3 cut(s) 201, 858, 4378
XmaJI CCTAGG 1 cut(s) 17
XmiI GTMKAC 1 cut(s) 3465
XmnI GAANNNNTTC 3 cut(s) 2268, 3209, 4513
XspI CTAG 7 cut(s) 18, 1706, 1958, 3867, 3983, 4301, 4439
ZrmI AGTACT 1 cut(s) 3981
Zsp2I ATGCAT 1 cut(s) 1889
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.