Basic Information

Type: gene

Biological Identity

Species

pyrus_communis

Chromosome

Chr6

Orthogroup ID

OG0003148

Physical Location & Seq

Genomic Coordinates Reverse (-)

22323016 .. 22328279

Gene Structure 5264 bp

Loading structure...

UTR

Exon/CDS

Intron

Transcript / Protein ID

N/A

Sequence Viewer

CDS Protein Promoter (2k)

Length: 3588 bp

ATGGGAGGTGGGGGTAGAAGAAAAAAGCAGCATTTTAGTCGAATCCATGCTTTTTCACGTGGGAAAGCTTCCTTCAATGGCGAGCATTCACGGATTGGCGGCCCTGGTTTCTCGAGGGTAGTTTACTGTAATGACCCTGAGTGCTTGGAGGCCACACTCCACAGCTACCAGGGCAATTATGTCAGAACGACTAAGTATACACTTGCTACATTCTTGCCCAAGGCAGTGTTTGAGCAGTTTAGAAGGGTTGCCAATTTATACTTCCTCATTTGTGCAATTCTGTCTTTTACGCCGCTTTCGCCTTACTCCGCCGTCAGCAACGTCGTCCCTCTCGTCGTCGTGATCGGAGTTACGATGGGGAAGGAGGCCGTTGAAGATTGGAGGAGGAAAAAACAGGACATTGAAATGAACAACAGCAAAGTTAAGGTGCATCATGGTGAGGGCAATTTTGAGCATACCAAATGGAGGGATTTGAAAGTTGGGGATATAGTGAAGGTCGAAAAGGACGGGTTTTTTCCTGCTGATCTTATCTTACTTTCATCAAGCTATGACGAAGCGCTTTGCTATGTTGAGACCACCAATCTTGATGGGGAAACCAATTTGAAACTAAAACAAGCACTGGAAGAGACTTCTAATCTGCACGAAGACTCAAGCTTTGAGAATTTTAAGGCGGTAATCAGATGTGAAGACCCAAATGCAAATTTGTACTCATTTGTTGGCAGTATGGAGATTGAAGATCAACCGTACCCTCTTACACCACAGCAGCTTCTGCTTCGAGACTCAAAGCTGCGAAACACAGATTTTGTATATGGGGTGGTAATCTTCACAGGTCACGATACAAAAGTTATGCAGAATTCAACAGATCCTCCTTCTAAGAGAAGTAAAGTTGAGAAGCGGATGGATAAGATTATCTACTTCTTGTTTTTCCTCTTGGTTGTGATGTCTTTAGTTGGGGCTATTGTCTTCGGGGTTAATACTAGTAAAGACCTGGATGATGGGAAAATGATCAGATGGTACCTCAGACCAGATGATACCACAGTTTACTATGATCCGACGAGGGCACCACTTGCAGCAAGTTTGCAATTTTTAACTGCCATTATGCTGTATAGTTATTTGATCCCCATTTCCTTGTATGTGTCAATAGAAATTGTCAAAGTTCTTCAGTCCACTTTTATAAACCAGGATGTGCACATGTATTATGAGGAAACTGACAAGCCAGCCCGAGCGCGTACTTCAAACTTGAATGAAGAACTTGGCCAAGTTGATACTATACTTTCAGATAAAACCGGAACATTAACTTGCAACTCGATGGAATTTATCAAGTGTTCTATTGCTGGCACTGCTTTTGGGCGCGGAGTTACAGAAGTTGAGAGAGCCTTAGCCAGGAGAAAAGGTGAGGAATTGACAGAAGAAGAGATCCATGCTGAAGAAGAAGAACTGAATGGAGCCAAGTCGACGATAAAAGGCTTTAATTTTATGGATGAAAGGATTATGAACGGAAAATGGGTTAATGAGCCTCGCGCAGATGTAATCCAGAAGTTTCTACAGTTACTAGCAATCTGTCATACCGCAATACCTGAAGTTGATGAAGAAACTGGAAGAGTATCATATGAAGCTGAATCACCAGATGAGGCAGCTTTCGTGATTGCAGCAAGAGAGTTTGGGTTTGAATTTTACGAAAGGACTCAAGGAAGCATCTCACTGCATGAGTTGGATCCCATTTATGGAAGAAAAATTGAAAGAAATTATAAACTTTTGAACATATTGGAGTTTAGTAGCTCAAGAAAGAGAATGTCCGTGATCATAAGAAACGAAGAGGGAAAGATACTACTCCTATGCAAAGGCGCTGACAGTGTCATGTTCGAAAGACTTGCAAAGAACGGAAGGGAGTTTGAAGAGAAGACTAGGGAACACATTAACAAGTATGCTGACGCTGGTTTGAGGACTTTGGTACTTGCATATCGCGAACTTGATGAAGAAGAATATGATGAGTTCAATAAAGAATTCACTGAGGCCAAAACCTTGGTGAGTTCAGATCGGGAGGAAATTGTCGAGGCAGTTTCAGAGAAGATTGAGAGGGATTTGATTCTTCTTGGTGCTACTGCAGTTGAAGACAAACTTCAAAATGGGGTTCCTGAATGCATTGACAAGCTTGCTCAGGCTGGAATTAAAATATGGGTTCTGACTGGAGATAAAATGGAGACAGCGATTAATATTGGATATGCGTGCAGTTTGCTTAGACAAGGAATGAAACAAATAGTAATAAGCTCCGAGACTGCAGAAGCTAAAGCATTAGAGAAGGTGGAGGATAAGACGGCAGTTGCCAAGGCATTGAAGGAAAGTGTAGTCCATCAAATAAGTCAGGGAAAGGCGTTGCTTGCTGCACCAGAAGAGAACTCTCAGGCACTGGCTTTGATCATTGATGGGAATTCGCTTGCTTATGCCTTAGAGAACGATGTCAAGATCGATCTCTTTCTAGAGCTTGCCATCGGCTGCGCATCTGTTATATGCTGTCGTTCGTCTCCCAAACAGAAAGCACTTGTTGCAAGACTGGTAAAAGATAAAACTGGCAACACAACTCTGGCCGTTGGTGATGGGGCAAATGATGTTGGAATGCTTCAAGAAGCAGATATCGGAGTTGGTATCAGCGGTGTTGAAGGAATGCAGGCAGTCATGTCAAGCGACATTGCAATTGCACAGTTCCGATTTTTGGAGCGCCTACTACTTGTACATGGCCATTGGTGTTACAGAAGAATCTCATCAATGGTCTGCTATTTCTTCTACAAGAACATTGCTTTTGGCTTCACTATTTTCTTCTATCAGATATATGCTTCATTCTCGGGCCAAACTGCGTACAACGATTGGTATCTGTCACTATACAATGTCTTCTTCACATCACTTCCGGTGATTGCCTTGGGAGTTTTTGACCAAGATGTCTCGGCCAAGTTCTGTCTCAAGTTCCCGCTATTGTACCAAGAAGGTGCGCAAAACGTCTTATTTAGCTGGCTCCGGATACTTGGATGGGCAATGAACGGGGCGGTGACTGCTACCCTAGTCTTCTTCTTCTGCGTTGTGGCAATGGGCAGTCAAGCCTTTCGTAAAGGCGGGCAAGTTGTTGGCTTGGAAATCTTTGGTGCCACGATGTACTCATGTGTAGTATGGGTTGTCAACTGTCAAATGGCACTTTCCATCAACTACTTCACTTATATACAACATCTCTTCATCTGGGGTGGCATCATATTCTGGTATATATTCCAGTTGGCATATGGAGCCATTGACCCTGACATATCAACCACTGCGTATAAGGTGTTCATTGAAGCCTGCGCACCAGCTCCGTTTTATTGGCTTCTCACACTGTTTGTTTTGGTCTCTTCCCTCCTGCCATACTTCGCTTACGCAGCCATCCAAATGCGGTTTTTCCCCATGTATCATCAGATGATACAGTGGATAAGAACAGATGGGCAATCAGATGACCCCGAGTTCTGCCAAATGGTGCGGCAGAGATCCATAAGAAGCACAACAGTAGGTTACACAGCCCGCATTGAAGCAACATCTAAACGCTTTGTAGAGAAGCCCGAAGGTCATTAA

Functional Annotation

Gene Ontology

Molecular Function
Biological Process
Cellular Component

GO:0003674 GO:0003824 GO:0004012 GO:0005215 GO:0005319 GO:0005548 GO:0005575 GO:0005622 GO:0005623 GO:0005737 GO:0005794 GO:0005802 GO:0005886 GO:0006810 GO:0006811 GO:0006820 GO:0006869 GO:0006900 GO:0006996 GO:0008150 GO:0009987 GO:0010876 GO:0012505 GO:0015711 GO:0015748 GO:0015914 GO:0016020 GO:0016043 GO:0016050 GO:0016192 GO:0016462 GO:0016787 GO:0016817 GO:0016818 GO:0016887 GO:0017111 GO:0031984 GO:0033036 GO:0034204 GO:0042623 GO:0043226 GO:0043227 GO:0043229 GO:0043231 GO:0043492 GO:0044422 GO:0044424 GO:0044431 GO:0044444 GO:0044446 GO:0044464 GO:0045332 GO:0048193 GO:0048194 GO:0051179 GO:0051234 GO:0061024 GO:0065007 GO:0065008 GO:0071702 GO:0071840 GO:0071944 GO:0097035 GO:0098791

KEGG Pathways

Metabolic & Signaling

No pathways identified.

Pfam Domains

Protein Families

Cation_ATPase E1-E2_ATPase Hydrolase PhoLip_ATPase_C PhoLip_ATPase_N

Protein Analysis

1196

Amino Acids

134.91

Weight (kDa)

5.52

Isoelectric Point (pI)

39.47

Instability Index

Protein Domains (Pfam)

Domain Name	Pfam ID	Position	E-value	Description
PhoLip_ATPase_N	PF16209	41 - 106	5.2e-23	Phospholipid-translocating ATPase N-terminal
Cation_ATPase	PF13246	512 - 708	1.9e-21	P-type ATPase, cytoplasmic domain N
PhoLip_ATPase_C	PF16212	891 - 1141	2.5e-86	Phospholipid-translocating P-type ATPase C-terminal

Hydrophobicity Profile (Kyte-Doolittle)

AI Structure Prediction Report

Calculating structure properties...

Genomic Context

Gene Family Tree

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Align Labels (Cladogram)

Image

Tree File

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Publication-ready

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Orthologous Genes (Group: OG0003148)

Species	Orthologous Gene IDs
arabidopsis_thaliana	AT1G13210 AT1G26130 AT1G26130 AT1G26130 AT1G26130 AT1G68710 AT1G68710 AT1G68710 AT3G25610
fragaria_vesca	FvH4_4g28450 FvH4_5g12920 FvH4_5g12920
malus_domestica	MD06G1209100.v1.1 MD13G1050400.v1.1 MD14G1219500.v1.1 MD16G1051100.v1.1
prunus_persica	Prupe.1G301500_v2.0.a1 Prupe.5G214200_v2.0.a1
pyrus_communis	pycom06g18670 pycom14g18190 pycom16g04490
rosa_chinensis	RchiOBHm_Chr4g0436411 RchiOBHm_Chr7g0184301
rosa_laevigata	RLG00000004988 RLG00000006521
rosa_multiflora	Rmu_sc0000579.1_g000001 Rmu_sc0013812.1_g000007
rosa_roxburghii	Rroxscaffold_3G00269750 Rroxscaffold_5G00377570
rosa_rugosa	Rorug04G0293600
rosa_samantha	Rh4AG348000 Rh4CG371300 Rh7AG081300 Rh7CG066300 Rh7DG065900
rosa_wichuraiana	Rw4G030420 Rw7G005410

Restriction Enzyme Sites

1 / 10

Enzyme	Recognition Site	Cut Count	Positions (bp)
AanI	TTATAA	2 cut(s)	1175, 1749
AasI	GACNNNNNNGTC	1 cut(s)	2933
Acc16I	TGCGCA	3 cut(s)	2497, 2985, 3325
Acc65I	GGTACC	1 cut(s)	1014
AccB1I	GGYRCC	3 cut(s)	1014, 1060, 3134
AccI	GTMKAC	2 cut(s)	197, 1454
AccII	CGCG	4 cut(s)	1228, 1353, 1521, 1965
AccIII	TCCGGA	1 cut(s)	3009
AclWI	GGATC	7 cut(s)	857, 1043, 1111, 1411, 1709, 1722, 3498
AcoI	YGGCCR	4 cut(s)	1255, 2583, 2734, 2940
AcsI	RAATTY	7 cut(s)	661, 700, 853, 1313, 1670, 2003, 2428
AcuI	CTGAAG	3 cut(s)	1145, 1446, 1599
AcvI	CACGTG	1 cut(s)	59
AfaI	GTAC	9 cut(s)	707, 746, 1016, 1231, 1953, 2730, 2855, 2972, 3146
AfeI	AGCGCT	1 cut(s)	558
AfiI	CCNNNNNNNGG	4 cut(s)	465, 1383, 1724, 2710
AflIII	ACRYGT	1 cut(s)	1191
AhlI	ACTAGT	1 cut(s)	977
AjnI	CCWGG	5 cut(s)	103, 168, 987, 1179, 1382
AjuI	GAANNNNNNNTTGG	2 cut(s)	245, 277
Alw21I	GWGCWC	1 cut(s)	1191
Alw26I	GTCTC	9 cut(s)	566, 620, 771, 2195, 2267, 2526, 2941, 2957, 3373
Alw44I	GTGCAC	1 cut(s)	1187
AlwI	GGATC	7 cut(s)	857, 1043, 1111, 1411, 1709, 1722, 3498
AlwNI	CAGNNNCTG	2 cut(s)	769, 2407
Ama87I	CYCGRG	4 cut(s)	112, 1221, 2839, 3476
Aor13HI	TCCGGA	1 cut(s)	3009
Aor51HI	AGCGCT	1 cut(s)	558
AoxI	GGCC	9 cut(s)	100, 150, 366, 1255, 2013, 2583, 2734, 2842, 2940
ApaLI	GTGCAC	1 cut(s)	1187
ApeKI	GCWGC	9 cut(s)	28, 763, 787, 1070, 1634, 1649, 2381, 2493, 3398
ApoI	RAATTY	7 cut(s)	661, 700, 853, 1313, 1670, 2003, 2428
ArsI	GACNNNNNNTTYG	2 cut(s)	638, 670
AseI	ATTAAT	1 cut(s)	2211
Asp718I	GGTACC	1 cut(s)	1014
AspLEI	GCGC	9 cut(s)	559, 1228, 1353, 1523, 1847, 2498, 2718, 2986, 3326
AspS9I	GGNCC	2 cut(s)	101, 2842
AsuHPI	GGTGA	7 cut(s)	449, 1406, 1614, 2038, 2603, 2917, 3052
AsuII	TTCGAA	1 cut(s)	1863
AvaI	CYCGRG	4 cut(s)	112, 1221, 2839, 3476
BaeGI	GKGCMC	2 cut(s)	1063, 1191
BaeI	ACNNNNGTAYC	4 cut(s)	828, 861, 1023, 1056
BalI	TGGCCA	2 cut(s)	1257, 2736
BamHI	GGATCC	1 cut(s)	1714
BanI	GGYRCC	3 cut(s)	1014, 1060, 3134
BbrPI	CACGTG	1 cut(s)	59
BbsI	GAAGAC	7 cut(s)	651, 693, 955, 1907, 2118, 2878, 3049
Bbv12I	GWGCWC	1 cut(s)	1191
BbvI	GCAGC	9 cut(s)	40, 774, 775, 1082, 1646, 1661, 2368, 2480, 3410
BceAI	ACGGC	4 cut(s)	296, 353, 2331, 2570
BcgI	CGANNNNNNTGC	4 cut(s)	20, 54, 1853, 1887
BciT130I	CCWGG	5 cut(s)	105, 170, 989, 1181, 1384
BciVI	GTATCC	1 cut(s)	3006
BclI	TGATCA	3 cut(s)	1005, 1800, 2415
BcoDI	GTCTC	9 cut(s)	566, 620, 771, 2195, 2267, 2526, 2941, 2957, 3373
BcuI	ACTAGT	1 cut(s)	977
BfaI	CTAG	5 cut(s)	978, 1553, 1905, 2477, 3053
BfmI	CTRYAG	3 cut(s)	1544, 2103, 2277
BfoI	RGCGCY	3 cut(s)	560, 1848, 2719
BfuI	GTATCC	1 cut(s)	3006
Bme1390I	CCNGG	5 cut(s)	105, 170, 989, 1181, 1384
BmeT110I	CYCGRG	4 cut(s)	112, 1221, 2839, 3476
BmgT120I	GGNCC	2 cut(s)	101, 2842
BmiI	GGNNCC	8 cut(s)	1016, 1062, 1447, 1716, 2133, 3008, 3136, 3271
BmrFI	CCNGG	5 cut(s)	105, 170, 989, 1181, 1384
BmsI	GCATC	4 cut(s)	439, 1704, 2507, 3243
BpiI	GAAGAC	7 cut(s)	651, 693, 955, 1907, 2118, 2878, 3049
BpmI	CTGGAG	1 cut(s)	2208
Bpu10I	CCTNAGC	2 cut(s)	1378, 2157
Bpu14I	TTCGAA	1 cut(s)	1863
BpuEI	CTTGAG	4 cut(s)	634, 1671, 1765, 2939
Bsa29I	ATCGAT	1 cut(s)	2466
BsaAI	YACGTR	1 cut(s)	59
BsaBI	GATNNNNATC	1 cut(s)	2865
BsaI	GGTCTC	2 cut(s)	566, 3373
BsaJI	CCNNGG	6 cut(s)	103, 169, 219, 2022, 2325, 2913
BsaWI	WCCGGW	3 cut(s)	1286, 2902, 3009
BsaXI	ACNNNNNCTCC	4 cut(s)	850, 880, 1378, 1408
Bsc4I	CCNNNNNNNGG	4 cut(s)	465, 1383, 1724, 2710
Bse1I	ACTGG	7 cut(s)	624, 1600, 2191, 2412, 2556, 2572, 3256
Bse3DI	GCAATG	4 cut(s)	2685, 2790, 3033, 3084
Bse8I	GATNNNNATC	1 cut(s)	2865
BseAI	TCCGGA	1 cut(s)	3009
BseBI	CCWGG	5 cut(s)	105, 170, 989, 1181, 1384
BseCI	ATCGAT	1 cut(s)	2466
BseDI	CCNNGG	6 cut(s)	103, 169, 219, 2022, 2325, 2913
BseGI	GGATG	6 cut(s)	903, 997, 1189, 1486, 3026, 3402
BseJI	GATNNNNATC	1 cut(s)	2865
BseLI	CCNNNNNNNGG	4 cut(s)	465, 1383, 1724, 2710
BseMI	GCAATG	4 cut(s)	2685, 2790, 3033, 3084
BseMII	CTCAG	5 cut(s)	129, 1033, 2001, 2171, 2414
BseNI	ACTGG	7 cut(s)	624, 1600, 2191, 2412, 2556, 2572, 3256
BseRI	GAGGAG	1 cut(s)	397
BseSI	GKGCMC	2 cut(s)	1063, 1191
BseXI	GCAGC	9 cut(s)	40, 774, 775, 1082, 1646, 1661, 2368, 2480, 3410
BsgI	GTGCAG	3 cut(s)	623, 2248, 2367
Bsh1236I	CGCG	4 cut(s)	1228, 1353, 1521, 1965
BshFI	GGCC	9 cut(s)	102, 152, 368, 1257, 2015, 2585, 2736, 2844, 2942
BshNI	GGYRCC	3 cut(s)	1014, 1060, 3134
BshVI	ATCGAT	1 cut(s)	2466
BsiHKAI	GWGCWC	1 cut(s)	1191
BsiHKCI	CYCGRG	4 cut(s)	112, 1221, 2839, 3476
BsiSI	CCGG	3 cut(s)	1287, 2903, 3010
BslFI	GGGAC	1 cut(s)	311
BslI	CCNNNNNNNGG	4 cut(s)	465, 1383, 1724, 2710
BsmAI	GTCTC	9 cut(s)	566, 620, 771, 2195, 2267, 2526, 2941, 2957, 3373
BsmBI	CGTCTC	1 cut(s)	2526
BsmFI	GGGAC	1 cut(s)	311
BsmI	GAATGC	4 cut(s)	85, 2144, 2619, 2667
BsnI	GGCC	9 cut(s)	102, 152, 368, 1257, 2015, 2585, 2736, 2844, 2942
Bso31I	GGTCTC	2 cut(s)	566, 3373
BsoBI	CYCGRG	4 cut(s)	112, 1221, 2839, 3476
Bsp119I	TTCGAA	1 cut(s)	1863
Bsp1286I	GDGCHC	2 cut(s)	1063, 1191
Bsp13I	TCCGGA	1 cut(s)	3009
Bsp1407I	TGTACA	1 cut(s)	2728
Bsp68I	TCGCGA	1 cut(s)	1965
BspANI	GGCC	9 cut(s)	102, 152, 368, 1257, 2015, 2585, 2736, 2844, 2942
BspCNI	CTCAG	5 cut(s)	130, 1032, 2002, 2170, 2413
BspDI	ATCGAT	1 cut(s)	2466
BspEI	TCCGGA	1 cut(s)	3009
BspFNI	CGCG	4 cut(s)	1228, 1353, 1521, 1965
BspLI	GGNNCC	8 cut(s)	1016, 1062, 1447, 1716, 2133, 3008, 3136, 3271
BspMAI	CTGCAG	2 cut(s)	2107, 2281
BspPI	GGATC	7 cut(s)	857, 1043, 1111, 1411, 1709, 1722, 3498
BspT104I	TTCGAA	1 cut(s)	1863
BspT107I	GGYRCC	3 cut(s)	1014, 1060, 3134
BspTNI	GGTCTC	2 cut(s)	566, 3373
BsrDI	GCAATG	4 cut(s)	2685, 2790, 3033, 3084
BsrGI	TGTACA	1 cut(s)	2728
BsrI	ACTGG	7 cut(s)	624, 1600, 2191, 2412, 2556, 2572, 3256
BssECI	CCNNGG	6 cut(s)	103, 169, 219, 2022, 2325, 2913
BssNAI	GTATAC	1 cut(s)	198
BssT1I	CCWWGG	4 cut(s)	219, 2022, 2325, 2913
Bst1107I	GTATAC	1 cut(s)	198
Bst2UI	CCWGG	5 cut(s)	105, 170, 989, 1181, 1384
Bst6I	CTCTTC	8 cut(s)	618, 1407, 1594, 1809, 1890, 2385, 3224, 3376
BstAPI	GCANNNNNTGC	3 cut(s)	769, 1067, 2543
BstAUI	TGTACA	1 cut(s)	2728
BstBAI	YACGTR	1 cut(s)	59
BstBI	TTCGAA	1 cut(s)	1863
BstENI	CCTNNNNNAGG	1 cut(s)	1381
BstF5I	GGATG	6 cut(s)	903, 997, 1189, 1486, 3026, 3402
BstFNI	CGCG	4 cut(s)	1228, 1353, 1521, 1965
BstH2I	RGCGCY	3 cut(s)	560, 1848, 2719
BstHHI	GCGC	9 cut(s)	559, 1228, 1353, 1523, 1847, 2498, 2718, 2986, 3326
BstMAI	GTCTC	9 cut(s)	566, 620, 771, 2195, 2267, 2526, 2941, 2957, 3373
BstNI	CCWGG	5 cut(s)	105, 170, 989, 1181, 1384
BstNSI	RCATGY	1 cut(s)	1195
BstSCI	CCNGG	5 cut(s)	103, 168, 987, 1179, 1382
BstSFI	CTRYAG	3 cut(s)	1544, 2103, 2277
BstSLI	GKGCMC	2 cut(s)	1063, 1191
BstUI	CGCG	4 cut(s)	1228, 1353, 1521, 1965
BstV1I	GCAGC	9 cut(s)	40, 774, 775, 1082, 1646, 1661, 2368, 2480, 3410
BstV2I	GAAGAC	7 cut(s)	651, 693, 955, 1907, 2118, 2878, 3049
BstX2I	RGATCY	4 cut(s)	862, 1416, 1714, 3503
BstXI	CCANNNNNNTGG	1 cut(s)	2023
BstYI	RGATCY	4 cut(s)	862, 1416, 1714, 3503
BstZ17I	GTATAC	1 cut(s)	198
Bsu15I	ATCGAT	1 cut(s)	2466
BsuI	GTATCC	1 cut(s)	3006
BsuRI	GGCC	9 cut(s)	102, 152, 368, 1257, 2015, 2585, 2736, 2844, 2942
BsuTUI	ATCGAT	1 cut(s)	2466
BtsCI	GGATG	6 cut(s)	903, 997, 1189, 1486, 3026, 3402
BtsI	GCAGTG	4 cut(s)	231, 1338, 1700, 3294
BtuMI	TCGCGA	1 cut(s)	1965
CaiI	CAGNNNCTG	2 cut(s)	769, 2407
CfoI	GCGC	9 cut(s)	559, 1228, 1353, 1523, 1847, 2498, 2718, 2986, 3326
Cfr13I	GGNCC	2 cut(s)	101, 2842
ClaI	ATCGAT	1 cut(s)	2466
CseI	GACGC	1 cut(s)	1940
Csp6I	GTAC	9 cut(s)	706, 745, 1015, 1230, 1952, 2729, 2854, 2971, 3145
CspCI	CAANNNNNGTGG	2 cut(s)	565, 600
CviQI	GTAC	9 cut(s)	706, 745, 1015, 1230, 1952, 2729, 2854, 2971, 3145
DrdI	GACNNNNNNGTC	1 cut(s)	2933
DseDI	GACNNNNNNGTC	1 cut(s)	2933
EaeI	YGGCCR	4 cut(s)	1255, 2583, 2734, 2940
Eam1104I	CTCTTC	8 cut(s)	618, 1407, 1594, 1809, 1890, 2385, 3224, 3376
EarI	CTCTTC	8 cut(s)	618, 1407, 1594, 1809, 1890, 2385, 3224, 3376
EciI	GGCGGA	1 cut(s)	298
Eco130I	CCWWGG	4 cut(s)	219, 2022, 2325, 2913
Eco31I	GGTCTC	2 cut(s)	566, 3373
Eco32I	GATATC	1 cut(s)	2632
Eco47III	AGCGCT	1 cut(s)	558
Eco57I	CTGAAG	3 cut(s)	1145, 1446, 1599
Eco72I	CACGTG	1 cut(s)	59
Eco88I	CYCGRG	4 cut(s)	112, 1221, 2839, 3476
EcoNI	CCTNNNNNAGG	1 cut(s)	1381
EcoRI	GAATTC	3 cut(s)	853, 2003, 2428
EcoRII	CCWGG	5 cut(s)	103, 168, 987, 1179, 1382
EcoRV	GATATC	1 cut(s)	2632
EcoT14I	CCWWGG	4 cut(s)	219, 2022, 2325, 2913
EcoT22I	ATGCAT	1 cut(s)	2144
ErhI	CCWWGG	4 cut(s)	219, 2022, 2325, 2913
Esp3I	CGTCTC	1 cut(s)	2526
FalI	AAGNNNNNCTT	2 cut(s)	2103, 2135
FaqI	GGGAC	1 cut(s)	311
FauI	CCCGC	3 cut(s)	2970, 3098, 3545
FauNDI	CATATG	2 cut(s)	1609, 3265
FbaI	TGATCA	3 cut(s)	1005, 1800, 2415
FblI	GTMKAC	2 cut(s)	197, 1454
FokI	GGATG	6 cut(s)	910, 1004, 1196, 1493, 3033, 3389
FspBI	CTAG	5 cut(s)	978, 1553, 1905, 2477, 3053
FspI	TGCGCA	3 cut(s)	2497, 2985, 3325
GlaI	GCGC	9 cut(s)	558, 1227, 1352, 1522, 1846, 2497, 2717, 2985, 3325
GsuI	CTGGAG	1 cut(s)	2208
HaeII	RGCGCY	3 cut(s)	560, 1848, 2719
HaeIII	GGCC	9 cut(s)	102, 152, 368, 1257, 2015, 2585, 2736, 2844, 2942
HapII	CCGG	3 cut(s)	1287, 2903, 3010
HgaI	GACGC	1 cut(s)	1940
HhaI	GCGC	9 cut(s)	559, 1228, 1353, 1523, 1847, 2498, 2718, 2986, 3326
Hin6I	GCGC	9 cut(s)	557, 1226, 1351, 1521, 1845, 2496, 2716, 2984, 3324
HinP1I	GCGC	9 cut(s)	557, 1226, 1351, 1521, 1845, 2496, 2716, 2984, 3324
HincII	GTYRAC	2 cut(s)	1455, 3169
HindII	GTYRAC	2 cut(s)	1455, 3169
HindIII	AAGCTT	3 cut(s)	66, 652, 2150
HinfI	GANTC	7 cut(s)	42, 647, 779, 1619, 1684, 2086, 2754
HpaII	CCGG	3 cut(s)	1287, 2903, 3010
HphI	GGTGA	7 cut(s)	449, 1406, 1614, 2038, 2603, 2917, 3052
Hpy166II	GTNNAC	7 cut(s)	124, 198, 1042, 1167, 1189, 1455, 3169
Hpy8I	GTNNAC	7 cut(s)	124, 198, 1042, 1167, 1189, 1455, 3169
Hpy99I	CGWCG	5 cut(s)	326, 338, 341, 1057, 1459
HpyCH4IV	ACGT	3 cut(s)	58, 321, 2991
HpySE526I	ACGT	3 cut(s)	58, 321, 2991
HspAI	GCGC	9 cut(s)	557, 1226, 1351, 1521, 1845, 2496, 2716, 2984, 3324
Kpn2I	TCCGGA	1 cut(s)	3009
KpnI	GGTACC	1 cut(s)	1018
Ksp22I	TGATCA	3 cut(s)	1005, 1800, 2415
LmnI	GCTCC	6 cut(s)	1445, 2273, 2713, 3012, 3269, 3337
Lsp1109I	GCAGC	9 cut(s)	40, 774, 775, 1082, 1646, 1661, 2368, 2480, 3410
LweI	GCATC	4 cut(s)	439, 1704, 2507, 3243
MaeI	CTAG	5 cut(s)	978, 1553, 1905, 2477, 3053
MaeII	ACGT	3 cut(s)	58, 321, 2991
MaeIII	GTNAC	8 cut(s)	349, 830, 1357, 1548, 2744, 2871, 3040, 3527
MfeI	CAATTG	1 cut(s)	2691
MflI	RGATCY	4 cut(s)	862, 1416, 1714, 3503
MhlI	GDGCHC	2 cut(s)	1063, 1191
MlsI	TGGCCA	2 cut(s)	1257, 2736
MluNI	TGGCCA	2 cut(s)	1257, 2736
MlyI	GAGTC	3 cut(s)	641, 773, 1678
MmeI	TCCRAC	3 cut(s)	1076, 1692, 2590
Mox20I	TGGCCA	2 cut(s)	1257, 2736
Mph1103I	ATGCAT	1 cut(s)	2144
MroI	TCCGGA	1 cut(s)	3009
MscI	TGGCCA	2 cut(s)	1257, 2736
MslI	CAYNNNNRTG	3 cut(s)	404, 435, 3407
Msp20I	TGGCCA	2 cut(s)	1257, 2736
MspA1I	CMGCKG	1 cut(s)	2649
MspI	CCGG	3 cut(s)	1287, 2903, 3010
MspR9I	CCNGG	5 cut(s)	105, 170, 989, 1181, 1384
MunI	CAATTG	1 cut(s)	2691
Mva1269I	GAATGC	4 cut(s)	85, 2144, 2619, 2667
MvaI	CCWGG	5 cut(s)	105, 170, 989, 1181, 1384
MvnI	CGCG	4 cut(s)	1228, 1353, 1521, 1965
NdeI	CATATG	2 cut(s)	1609, 3265
NlaIV	GGNNCC	8 cut(s)	1016, 1062, 1447, 1716, 2133, 3008, 3136, 3271
NmeAIII	GCCGAG	1 cut(s)	2918
NmuCI	GTSAC	3 cut(s)	830, 2871, 3040
NruI	TCGCGA	1 cut(s)	1965
NsbI	TGCGCA	3 cut(s)	2497, 2985, 3325
NsiI	ATGCAT	1 cut(s)	2144
NspI	RCATGY	1 cut(s)	1195
NspV	TTCGAA	1 cut(s)	1863
PaeR7I	CTCGAG	1 cut(s)	112
PciI	ACATGT	1 cut(s)	1191
PcsI	WCGNNNNNNNCGW	1 cut(s)	330
PctI	GAATGC	4 cut(s)	85, 2144, 2619, 2667
PfeI	GAWTC	4 cut(s)	42, 1619, 2086, 2754
PflFI	GACNNNGTC	1 cut(s)	1853
PleI	GAGTC	3 cut(s)	641, 773, 1678
PmaCI	CACGTG	1 cut(s)	59
PmlI	CACGTG	1 cut(s)	59
PpsI	GAGTC	3 cut(s)	641, 773, 1678
Ppu21I	YACGTR	1 cut(s)	59
PscI	ACATGT	1 cut(s)	1191
PshBI	ATTAAT	1 cut(s)	2211
PsiI	TTATAA	2 cut(s)	1175, 1749
Psp6I	CCWGG	5 cut(s)	103, 168, 987, 1179, 1382
PspCI	CACGTG	1 cut(s)	59
PspGI	CCWGG	5 cut(s)	103, 168, 987, 1179, 1382
PspN4I	GGNNCC	8 cut(s)	1016, 1062, 1447, 1716, 2133, 3008, 3136, 3271
PspPI	GGNCC	2 cut(s)	101, 2842
PsrI	GAACNNNNNNTAC	2 cut(s)	3293, 3325
PstI	CTGCAG	2 cut(s)	2107, 2281
PstNI	CAGNNNCTG	2 cut(s)	769, 2407
PsuI	RGATCY	4 cut(s)	862, 1416, 1714, 3503
PsyI	GACNNNGTC	1 cut(s)	1853
RruI	TCGCGA	1 cut(s)	1965
RsaI	GTAC	9 cut(s)	707, 746, 1016, 1231, 1953, 2730, 2855, 2972, 3146
RsaNI	GTAC	9 cut(s)	706, 745, 1015, 1230, 1952, 2729, 2854, 2971, 3145
RseI	CAYNNNNRTG	3 cut(s)	404, 435, 3407
SalI	GTCGAC	1 cut(s)	1453
Sau96I	GGNCC	2 cut(s)	101, 2842
SchI	GAGTC	3 cut(s)	641, 773, 1678
ScrFI	CCNGG	5 cut(s)	105, 170, 989, 1181, 1384
SduI	GDGCHC	2 cut(s)	1063, 1191
SfaNI	GCATC	4 cut(s)	439, 1704, 2507, 3243
SfcI	CTRYAG	3 cut(s)	1544, 2103, 2277
Sfr274I	CTCGAG	1 cut(s)	112
SfuI	TTCGAA	1 cut(s)	1863
SlaI	CTCGAG	1 cut(s)	112
SmiMI	CAYNNNNRTG	3 cut(s)	404, 435, 3407
SmlI	CTYRAG	5 cut(s)	112, 649, 1686, 1780, 2954
SmoI	CTYRAG	5 cut(s)	112, 649, 1686, 1780, 2954
SpeI	ACTAGT	1 cut(s)	977
SspI	AATATT	1 cut(s)	2215
SspMI	CTAG	5 cut(s)	978, 1553, 1905, 2477, 3053
StyD4I	CCNGG	5 cut(s)	103, 168, 987, 1179, 1382
StyI	CCWWGG	4 cut(s)	219, 2022, 2325, 2913
TaiI	ACGT	3 cut(s)	61, 324, 2994
TaqI	TCGA	9 cut(s)	40, 113, 498, 775, 1307, 1454, 1863, 2052, 2466
TatI	WGTACW	3 cut(s)	705, 2728, 3144
TauI	GCSGC	3 cut(s)	102, 295, 3499
TfiI	GAWTC	4 cut(s)	42, 1619, 2086, 2754
TseFI	GTSAC	3 cut(s)	830, 2871, 3040
TseI	GCWGC	9 cut(s)	28, 763, 787, 1070, 1634, 1649, 2381, 2493, 3398
Tsp45I	GTSAC	3 cut(s)	830, 2871, 3040
TspGWI	ACGGA	5 cut(s)	106, 1512, 1786, 1896, 3324
Tth111I	GACNNNGTC	1 cut(s)	1853
VneI	GTGCAC	1 cut(s)	1187
VspI	ATTAAT	1 cut(s)	2211
XagI	CCTNNNNNAGG	1 cut(s)	1381
XapI	RAATTY	7 cut(s)	661, 700, 853, 1313, 1670, 2003, 2428
XbaI	TCTAGA	1 cut(s)	2476
XceI	RCATGY	1 cut(s)	1195
XcmI	CCANNNNNNNNNTGG	1 cut(s)	3263
XhoI	CTCGAG	1 cut(s)	112
XmiI	GTMKAC	2 cut(s)	197, 1454
XspI	CTAG	5 cut(s)	978, 1553, 1905, 2477, 3053
Zsp2I	ATGCAT	1 cut(s)	2144

Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.