pycom06g18970

ATP-dependent Clp protease adapter protein

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
pyrus_communis
Chr6
OG0004724
Physical Location & Seq
Forward (+)
22525935 .. 22528053
2119 bp
Loading structure...
UTR
Exon/CDS
Intron
N/A

Sequence Viewer

Length: 474 bp
ATGGCTGCCATCTGCGGTCGAGTCCCTCTTTCGACAAACCATGGATTGAATCTCACCAAATCAGGTGATAAACATTATTTTCATAGACAAAGTCCAAATCGGAGCATCTTGATGACAACATCAAGCACCAGGCTTGGCACAGGTGGTGGGGTGTTGGACAAGCCAACCATAGAGAAAACAAATCCTAGTCGTGACTCAGAGTTTGACGTGAGGAAATCAAGGAAAATGTCTCCACCGTACCGAGTGATGCTGCACAATGACAACTATAACAAGAGGGAGTACGTTGTGCAAGTTCTGATGAAGGTCATCCCGGGAATGACGGTTGACATTGCGGTGAACGTTATGCAGGAGGCACATCACAACGGCCTGGCCGTCGTAATTGTGTGCGCTCAGGTGGATGCAGAAGATCACTGCATGCAGCTCCGAGGCAACGGTCTTCTAAGCTCCGTTGAGCCCGCCGGTGATGGATGTTAA
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
No pathways identified.
Pfam Domains
Protein Families
ClpS

Protein Analysis

158

Amino Acids

17.23

Weight (kDa)

8.73

Isoelectric Point (pI)

47.57

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
ClpS PF02617 77 - 143 1.3e-17 ATP-dependent Clp protease adaptor protein ClpS
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

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Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AciI CCGC 3 cut(s) 15, 332, 456
AclI AACGTT 1 cut(s) 339
AcoI YGGCCR 1 cut(s) 369
AfaI GTAC 2 cut(s) 239, 281
AgsI TTSAA 1 cut(s) 49
AjiI CACGTC 1 cut(s) 208
AjnI CCWGG 2 cut(s) 128, 366
AluBI AGCT 2 cut(s) 421, 444
AluI AGCT 2 cut(s) 421, 444
Alw26I GTCTC 1 cut(s) 234
Ama87I CYCGRG 1 cut(s) 310
AoxI GGCC 2 cut(s) 364, 369
ApeKI GCWGC 3 cut(s) 5, 250, 418
ArsI GACNNNNNNTTYG 2 cut(s) 185, 217
AspLEI GCGC 1 cut(s) 389
AsuC2I CCSGG 2 cut(s) 311, 312
AsuHPI GGTGA 4 cut(s) 46, 77, 346, 473
AvaI CYCGRG 1 cut(s) 310
BanII GRGCYC 1 cut(s) 456
BbsI GAAGAC 1 cut(s) 428
BbvI GCAGC 2 cut(s) 237, 430
BccI CCATC 2 cut(s) 17, 458
BceAI ACGGC 2 cut(s) 356, 379
BciT130I CCWGG 2 cut(s) 130, 368
BcnI CCSGG 2 cut(s) 311, 312
BcoDI GTCTC 1 cut(s) 234
BfaI CTAG 1 cut(s) 186
BisI GCNGC 3 cut(s) 6, 251, 419
BlsI GCNGC 3 cut(s) 7, 252, 420
Bme1390I CCNGG 4 cut(s) 130, 311, 312, 368
BmeT110I CYCGRG 1 cut(s) 310
BmgBI CACGTC 1 cut(s) 208
BmrFI CCNGG 4 cut(s) 130, 311, 312, 368
BmsI GCATC 3 cut(s) 114, 237, 388
BpiI GAAGAC 1 cut(s) 428
Bpu10I CCTNAGC 1 cut(s) 390
BpuMI CCSGG 2 cut(s) 311, 312
BsaJI CCNNGG 3 cut(s) 40, 310, 424
Bse118I RCCGGY 1 cut(s) 458
Bse3DI GCAATG 1 cut(s) 327
BseBI CCWGG 2 cut(s) 130, 368
BseDI CCNNGG 3 cut(s) 40, 310, 424
BseGI GGATG 3 cut(s) 306, 403, 473
BseMI GCAATG 1 cut(s) 327
BseMII CTCAG 2 cut(s) 210, 404
BseXI GCAGC 2 cut(s) 237, 430
BsgI GTGCAG 1 cut(s) 236
Bsh1285I CGRYCG 1 cut(s) 19
BshFI GGCC 2 cut(s) 366, 371
BsiEI CGRYCG 1 cut(s) 19
BsiHKCI CYCGRG 1 cut(s) 310
BsiSI CCGG 2 cut(s) 311, 459
BslFI GGGAC 1 cut(s) 8
BsmAI GTCTC 1 cut(s) 234
BsmFI GGGAC 1 cut(s) 8
BsnI GGCC 2 cut(s) 366, 371
BsoBI CYCGRG 1 cut(s) 310
Bsp1286I GDGCHC 1 cut(s) 456
Bsp143I GATC 1 cut(s) 406
Bsp19I CCATGG 1 cut(s) 40
BspACI CCGC 3 cut(s) 15, 332, 456
BspANI GGCC 2 cut(s) 366, 371
BspCNI CTCAG 2 cut(s) 209, 403
BsrDI GCAATG 1 cut(s) 327
BsrFI RCCGGY 1 cut(s) 458
BssAI RCCGGY 1 cut(s) 458
BssECI CCNNGG 3 cut(s) 40, 310, 424
BssMI GATC 1 cut(s) 406
BssT1I CCWWGG 1 cut(s) 40
Bst2UI CCWGG 2 cut(s) 130, 368
Bst4CI ACNGT 3 cut(s) 237, 322, 434
BstC8I GCNNGC 2 cut(s) 416, 456
BstDEI CTNAG 3 cut(s) 196, 390, 440
BstDSI CCRYGG 1 cut(s) 40
BstF5I GGATG 3 cut(s) 306, 403, 473
BstHHI GCGC 1 cut(s) 389
BstKTI GATC 1 cut(s) 409
BstMAI GTCTC 1 cut(s) 234
BstMBI GATC 1 cut(s) 406
BstMCI CGRYCG 1 cut(s) 19
BstNI CCWGG 2 cut(s) 130, 368
BstNSI RCATGY 1 cut(s) 418
BstSCI CCNGG 4 cut(s) 128, 309, 310, 366
BstV1I GCAGC 2 cut(s) 237, 430
BstV2I GAAGAC 1 cut(s) 428
BsuRI GGCC 2 cut(s) 366, 371
BtgI CCRYGG 1 cut(s) 40
BtrI CACGTC 1 cut(s) 208
BtsCI GGATG 3 cut(s) 306, 403, 473
BtsI GCAGTG 1 cut(s) 409
BtsIMutI CAGTG 1 cut(s) 409
Cac8I GCNNGC 2 cut(s) 416, 456
CfoI GCGC 1 cut(s) 389
Cfr10I RCCGGY 1 cut(s) 458
Cfr9I CCCGGG 1 cut(s) 310
Csp6I GTAC 2 cut(s) 238, 280
CviAII CATG 2 cut(s) 41, 415
CviJI RGCY 8 cut(s) 5, 133, 163, 366, 371, 421, 444, 454
CviKI_1 RGCY 8 cut(s) 5, 133, 163, 366, 371, 421, 444, 454
CviQI GTAC 2 cut(s) 238, 280
DdeI CTNAG 3 cut(s) 196, 390, 440
DpnI GATC 1 cut(s) 408
DpnII GATC 1 cut(s) 406
EaeI YGGCCR 1 cut(s) 369
Eco130I CCWWGG 1 cut(s) 40
Eco24I GRGCYC 1 cut(s) 456
Eco88I CYCGRG 1 cut(s) 310
EcoRII CCWGG 2 cut(s) 128, 366
EcoT14I CCWWGG 1 cut(s) 40
EcoT38I GRGCYC 1 cut(s) 456
ErhI CCWWGG 1 cut(s) 40
FaeI CATG 2 cut(s) 44, 418
FaiI YATR 6 cut(s) 42, 84, 170, 267, 344, 416
FaqI GGGAC 1 cut(s) 8
FatI CATG 2 cut(s) 40, 414
FauI CCCGC 1 cut(s) 463
Fnu4HI GCNGC 3 cut(s) 6, 251, 419
FokI GGATG 2 cut(s) 293, 410
FriOI GRGCYC 1 cut(s) 456
Fsp4HI GCNGC 3 cut(s) 6, 251, 419
FspBI CTAG 1 cut(s) 186
GlaI GCGC 1 cut(s) 388
GluI GCNGC 3 cut(s) 6, 251, 419
HaeIII GGCC 2 cut(s) 366, 371
HapII CCGG 2 cut(s) 311, 459
HhaI GCGC 1 cut(s) 389
Hin1II CATG 2 cut(s) 44, 418
Hin6I GCGC 1 cut(s) 387
HinP1I GCGC 1 cut(s) 387
HincII GTYRAC 1 cut(s) 325
HindII GTYRAC 1 cut(s) 325
HinfI GANTC 3 cut(s) 21, 49, 194
HpaII CCGG 2 cut(s) 311, 459
HphI GGTGA 4 cut(s) 46, 77, 346, 473
Hpy166II GTNNAC 2 cut(s) 325, 337
Hpy188I TCNGA 4 cut(s) 102, 199, 297, 425
Hpy188III TCNNGA 2 cut(s) 109, 191
Hpy8I GTNNAC 2 cut(s) 325, 337
Hpy99I CGWCG 1 cut(s) 377
HpyAV CCTTC 1 cut(s) 295
HpyCH4III ACNGT 3 cut(s) 237, 322, 434
HpyCH4IV ACGT 3 cut(s) 207, 282, 339
HpyCH4V TGCA 6 cut(s) 253, 289, 346, 401, 414, 418
HpyF3I CTNAG 3 cut(s) 196, 390, 440
HpySE526I ACGT 3 cut(s) 207, 282, 339
Hsp92II CATG 2 cut(s) 44, 418
HspAI GCGC 1 cut(s) 387
Kzo9I GATC 1 cut(s) 406
LmnI GCTCC 3 cut(s) 102, 426, 449
LpnPI CCDG 9 cut(s) 48, 115, 126, 142, 324, 332, 353, 377, 380
Lsp1109I GCAGC 2 cut(s) 237, 430
LweI GCATC 3 cut(s) 114, 237, 388
MaeI CTAG 1 cut(s) 186
MaeII ACGT 3 cut(s) 207, 282, 339
MaeIII GTNAC 1 cut(s) 191
MalI GATC 1 cut(s) 408
MboI GATC 1 cut(s) 406
MboII GAAGA 2 cut(s) 416, 428
MhlI GDGCHC 1 cut(s) 456
MluCI AATT 1 cut(s) 378
MlyI GAGTC 2 cut(s) 30, 188
MmeI TCCRAC 1 cut(s) 135
MnlI CCTC 5 cut(s) 36, 204, 267, 343, 419
MseI TTAA 1 cut(s) 472
MslI CAYNNNNRTG 2 cut(s) 110, 332
MspI CCGG 2 cut(s) 311, 459
MspR9I CCNGG 4 cut(s) 130, 311, 312, 368
MvaI CCWGG 2 cut(s) 130, 368
NciI CCSGG 2 cut(s) 311, 312
NcoI CCATGG 1 cut(s) 40
NdeII GATC 1 cut(s) 406
NlaIII CATG 2 cut(s) 44, 418
NmuCI GTSAC 1 cut(s) 191
NspI RCATGY 1 cut(s) 418
PaeI GCATGC 1 cut(s) 418
PcsI WCGNNNNNNNCGW 1 cut(s) 369
PfeI GAWTC 1 cut(s) 49
PflFI GACNNNGTC 1 cut(s) 90
PkrI GCNGC 3 cut(s) 7, 252, 420
PleI GAGTC 2 cut(s) 29, 188
PpsI GAGTC 2 cut(s) 29, 188
Psp1406I AACGTT 1 cut(s) 339
Psp6I CCWGG 2 cut(s) 128, 366
PspGI CCWGG 2 cut(s) 128, 366
PsyI GACNNNGTC 1 cut(s) 90
RsaI GTAC 2 cut(s) 239, 281
RsaNI GTAC 2 cut(s) 238, 280
RseI CAYNNNNRTG 2 cut(s) 110, 332
SaqAI TTAA 1 cut(s) 472
SatI GCNGC 3 cut(s) 6, 251, 419
Sau3AI GATC 1 cut(s) 406
SchI GAGTC 2 cut(s) 30, 188
ScrFI CCNGG 4 cut(s) 130, 311, 312, 368
SduI GDGCHC 1 cut(s) 456
SetI ASST 9 cut(s) 67, 145, 210, 285, 306, 342, 396, 423, 446
SfaNI GCATC 3 cut(s) 114, 237, 388
SgrAI CRCCGGYG 1 cut(s) 458
SmaI CCCGGG 1 cut(s) 312
SmiMI CAYNNNNRTG 2 cut(s) 110, 332
SphI GCATGC 1 cut(s) 418
Sse9I AATT 1 cut(s) 378
SsiI CCGC 3 cut(s) 15, 332, 456
SspMI CTAG 1 cut(s) 186
StyD4I CCNGG 4 cut(s) 128, 309, 310, 366
StyI CCWWGG 1 cut(s) 40
TaaI ACNGT 3 cut(s) 237, 322, 434
TaiI ACGT 3 cut(s) 210, 285, 342
TaqI TCGA 2 cut(s) 19, 32
TasI AATT 1 cut(s) 378
TfiI GAWTC 1 cut(s) 49
Tru1I TTAA 1 cut(s) 472
Tru9I TTAA 1 cut(s) 472
TscAI CASTG 1 cut(s) 416
TseFI GTSAC 1 cut(s) 191
TseI GCWGC 3 cut(s) 5, 250, 418
Tsp45I GTSAC 1 cut(s) 191
TspDTI ATGAA 2 cut(s) 71, 314
TspGWI ACGGA 1 cut(s) 436
TspMI CCCGGG 1 cut(s) 310
TspRI CASTG 1 cut(s) 416
Tth111I GACNNNGTC 1 cut(s) 90
XceI RCATGY 1 cut(s) 418
XmaI CCCGGG 1 cut(s) 310
XspI CTAG 1 cut(s) 186
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.