pycom07g06230

EF hand family protein

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
pyrus_communis
Chr7
OG0005854
Physical Location & Seq
Forward (+)
5516436 .. 5518644
2209 bp
Loading structure...
UTR
Exon/CDS
Intron
N/A

Sequence Viewer

Length: 498 bp
ATGGGACAGATCCTCGACAGATTACAAGGAAAGCAATGGAGGCGAAAGCAAATAAGGAGGATTACAGATAAGGTGTTTGATCATTTTAAAAATGAGACAGGAAGGGCTAGCTTGAATTTTGAAGATCTGTACATCGGTGTACTACTTGTGTACAATGATATCAACAAGCGTTTACCCGGCCCGCATTTTGATCGACCCTCCAAAGATCTAGTCAAAGCTATGATGCAGCAAAATGATCTCAATCTCGACGGAGAAATTGACCGGGAAGAGTTTGCGAAGTTCATGAAGCAGTTGACAGCAGAGACATTCGTTGTAGTCAGTCAGGGACTCATCCTCACACTGGTTGCAGCACCAGCGGTGGCAATGGCGACAAAAAGAGCTACCGAGGGCGTACCAGGGGTCGGAAAGGTCGTGCAAAGGTTACCCAATTCAGTTTATGCATCCCTTATAACTCTTACTGTCCTGTTGTTTCAGCAAGCGCGCCAGGAGTTCGATTAG
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
No pathways identified.
Pfam Domains
Protein Families
No domains found.

Protein Analysis

166

Amino Acids

18.76

Weight (kDa)

9.43

Isoelectric Point (pI)

45.31

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
DUF7874 PF25284 25 - 128 1.1e-06 Family of unknown function (DUF7874)
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
Loading...
Style Settings
Image
Tree File
Tip: Beautify your tree with professional tools

Download the Full Tree (.nwk) file, then upload it to any of the following tools to customize colors, fonts, annotations, clades, and branch support.

Publication-ready
iTOL Interactive Tree Of Life · Most popular EvolView Flexible annotations MEGA Desktop phylogenetics suite FigTree Desktop tree editor

Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AanI TTATAA 1 cut(s) 449
AccII CGCG 1 cut(s) 481
AciI CCGC 2 cut(s) 182, 356
AclWI GGATC 1 cut(s) 4
AcsI RAATTY 1 cut(s) 115
AfaI GTAC 4 cut(s) 131, 141, 152, 393
AfiI CCNNNNNNNGG 2 cut(s) 340, 401
AgsI TTSAA 2 cut(s) 115, 122
AjnI CCWGG 2 cut(s) 394, 483
AluBI AGCT 3 cut(s) 111, 218, 380
AluI AGCT 3 cut(s) 111, 218, 380
Alw26I GTCTC 2 cut(s) 89, 296
AlwI GGATC 1 cut(s) 4
AoxI GGCC 1 cut(s) 178
ApeKI GCWGC 2 cut(s) 226, 347
ApoI RAATTY 1 cut(s) 115
ArsI GACNNNNNNTTYG 2 cut(s) 195, 227
AspLEI GCGC 2 cut(s) 481, 483
AspS9I GGNCC 1 cut(s) 179
AsuC2I CCSGG 2 cut(s) 177, 263
AsuNHI GCTAGC 1 cut(s) 107
BbvI GCAGC 2 cut(s) 238, 359
BcgI CGANNNNNNTGC 2 cut(s) 173, 207
BciT130I CCWGG 2 cut(s) 396, 485
BclI TGATCA 1 cut(s) 79
BcnI CCSGG 2 cut(s) 177, 263
BcoDI GTCTC 2 cut(s) 89, 296
BfaI CTAG 2 cut(s) 108, 209
BglII AGATCT 2 cut(s) 124, 205
BisI GCNGC 2 cut(s) 227, 348
BlsI GCNGC 2 cut(s) 228, 349
Bme1390I CCNGG 4 cut(s) 177, 263, 396, 485
BmgT120I GGNCC 1 cut(s) 179
BmrFI CCNGG 4 cut(s) 177, 263, 396, 485
BmsI GCATC 2 cut(s) 213, 449
BmtI GCTAGC 1 cut(s) 111
BpuMI CCSGG 2 cut(s) 177, 263
BsaBI GATNNNNATC 1 cut(s) 240
BsaJI CCNNGG 2 cut(s) 384, 395
Bsc4I CCNNNNNNNGG 2 cut(s) 340, 401
Bse1I ACTGG 1 cut(s) 345
Bse3DI GCAATG 2 cut(s) 41, 369
Bse8I GATNNNNATC 1 cut(s) 240
BseBI CCWGG 2 cut(s) 396, 485
BseDI CCNNGG 2 cut(s) 384, 395
BseGI GGATG 2 cut(s) 330, 440
BseJI GATNNNNATC 1 cut(s) 240
BseLI CCNNNNNNNGG 2 cut(s) 340, 401
BseMI GCAATG 2 cut(s) 41, 369
BseNI ACTGG 1 cut(s) 345
BsePI GCGCGC 1 cut(s) 479
BseXI GCAGC 2 cut(s) 238, 359
Bsh1236I CGCG 1 cut(s) 481
BshFI GGCC 1 cut(s) 180
BsiSI CCGG 2 cut(s) 177, 262
BslFI GGGAC 2 cut(s) 18, 339
BslI CCNNNNNNNGG 2 cut(s) 340, 401
BsmAI GTCTC 2 cut(s) 89, 296
BsmFI GGGAC 2 cut(s) 18, 339
BsnI GGCC 1 cut(s) 180
Bsp1407I TGTACA 2 cut(s) 129, 150
Bsp143I GATC 6 cut(s) 9, 79, 124, 190, 205, 235
BspACI CCGC 2 cut(s) 182, 356
BspANI GGCC 1 cut(s) 180
BspFNI CGCG 1 cut(s) 481
BspHI TCATGA 1 cut(s) 282
BspOI GCTAGC 1 cut(s) 111
BspPI GGATC 1 cut(s) 4
BsrDI GCAATG 2 cut(s) 41, 369
BsrGI TGTACA 2 cut(s) 129, 150
BsrI ACTGG 1 cut(s) 345
BssECI CCNNGG 2 cut(s) 384, 395
BssHII GCGCGC 1 cut(s) 479
BssMI GATC 6 cut(s) 9, 79, 124, 190, 205, 235
Bst2UI CCWGG 2 cut(s) 396, 485
Bst4CI ACNGT 1 cut(s) 460
Bst6I CTCTTC 1 cut(s) 261
BstAUI TGTACA 2 cut(s) 129, 150
BstC8I GCNNGC 4 cut(s) 109, 182, 477, 481
BstEII GGTNACC 1 cut(s) 420
BstF5I GGATG 2 cut(s) 330, 440
BstFNI CGCG 1 cut(s) 481
BstHHI GCGC 2 cut(s) 481, 483
BstKTI GATC 6 cut(s) 12, 82, 127, 193, 208, 238
BstMAI GTCTC 2 cut(s) 89, 296
BstMBI GATC 6 cut(s) 9, 79, 124, 190, 205, 235
BstMWI GCNNNNNNNGC 2 cut(s) 40, 353
BstNI CCWGG 2 cut(s) 396, 485
BstPI GGTNACC 1 cut(s) 420
BstSCI CCNGG 4 cut(s) 175, 261, 394, 483
BstUI CGCG 1 cut(s) 481
BstV1I GCAGC 2 cut(s) 238, 359
BstX2I RGATCY 3 cut(s) 9, 124, 205
BstYI RGATCY 3 cut(s) 9, 124, 205
BsuRI GGCC 1 cut(s) 180
BtsCI GGATG 2 cut(s) 330, 440
BtsIMutI CAGTG 1 cut(s) 338
Cac8I GCNNGC 4 cut(s) 109, 182, 477, 481
CciI TCATGA 1 cut(s) 282
CfoI GCGC 2 cut(s) 481, 483
Cfr13I GGNCC 1 cut(s) 179
Csp6I GTAC 4 cut(s) 130, 140, 151, 392
CviAII CATG 1 cut(s) 283
CviJI RGCY 5 cut(s) 107, 111, 180, 218, 380
CviKI_1 RGCY 5 cut(s) 107, 111, 180, 218, 380
CviQI GTAC 4 cut(s) 130, 140, 151, 392
DpnI GATC 6 cut(s) 11, 81, 126, 192, 207, 237
DpnII GATC 6 cut(s) 9, 79, 124, 190, 205, 235
DraI TTTAAA 1 cut(s) 88
Eam1104I CTCTTC 1 cut(s) 261
EarI CTCTTC 1 cut(s) 261
Eco32I GATATC 1 cut(s) 160
Eco91I GGTNACC 1 cut(s) 420
EcoO65I GGTNACC 1 cut(s) 420
EcoRII CCWGG 2 cut(s) 394, 483
EcoRV GATATC 1 cut(s) 160
EcoT22I ATGCAT 1 cut(s) 442
FaeI CATG 1 cut(s) 286
FaiI YATR 4 cut(s) 221, 284, 438, 449
FaqI GGGAC 2 cut(s) 18, 339
FatI CATG 1 cut(s) 282
FauI CCCGC 1 cut(s) 189
FbaI TGATCA 1 cut(s) 79
Fnu4HI GCNGC 2 cut(s) 227, 348
FokI GGATG 2 cut(s) 317, 427
Fsp4HI GCNGC 2 cut(s) 227, 348
FspBI CTAG 2 cut(s) 108, 209
GlaI GCGC 2 cut(s) 480, 482
GluI GCNGC 2 cut(s) 227, 348
HaeIII GGCC 1 cut(s) 180
HapII CCGG 2 cut(s) 177, 262
HhaI GCGC 2 cut(s) 481, 483
Hin1II CATG 1 cut(s) 286
Hin6I GCGC 2 cut(s) 479, 481
HinP1I GCGC 2 cut(s) 479, 481
HincII GTYRAC 1 cut(s) 294
HindII GTYRAC 1 cut(s) 294
HinfI GANTC 1 cut(s) 327
HpaII CCGG 2 cut(s) 177, 262
Hpy166II GTNNAC 4 cut(s) 140, 151, 173, 294
Hpy188I TCNGA 1 cut(s) 404
Hpy188III TCNNGA 2 cut(s) 245, 283
Hpy8I GTNNAC 4 cut(s) 140, 151, 173, 294
Hpy99I CGWCG 1 cut(s) 251
HpyAV CCTTC 1 cut(s) 96
HpyCH4III ACNGT 1 cut(s) 460
HpyCH4V TGCA 4 cut(s) 226, 347, 415, 440
HpyF10VI GCNNNNNNNGC 2 cut(s) 40, 353
Hsp92II CATG 1 cut(s) 286
HspAI GCGC 2 cut(s) 479, 481
Ksp22I TGATCA 1 cut(s) 79
Kzo9I GATC 6 cut(s) 9, 79, 124, 190, 205, 235
Lsp1109I GCAGC 2 cut(s) 238, 359
LweI GCATC 2 cut(s) 213, 449
MaeI CTAG 2 cut(s) 108, 209
MaeIII GTNAC 1 cut(s) 420
MalI GATC 6 cut(s) 11, 81, 126, 192, 207, 237
MboI GATC 6 cut(s) 9, 79, 124, 190, 205, 235
MboII GAAGA 2 cut(s) 134, 278
MflI RGATCY 3 cut(s) 9, 124, 205
MluCI AATT 3 cut(s) 115, 255, 427
MlyI GAGTC 1 cut(s) 321
MmeI TCCRAC 1 cut(s) 382
MnlI CCTC 6 cut(s) 23, 33, 51, 208, 344, 379
Mph1103I ATGCAT 1 cut(s) 442
MseI TTAA 1 cut(s) 87
MspA1I CMGCKG 1 cut(s) 356
MspI CCGG 2 cut(s) 177, 262
MspR9I CCNGG 4 cut(s) 177, 263, 396, 485
MvaI CCWGG 2 cut(s) 396, 485
MvnI CGCG 1 cut(s) 481
MwoI GCNNNNNNNGC 2 cut(s) 40, 353
NciI CCSGG 2 cut(s) 177, 263
NdeII GATC 6 cut(s) 9, 79, 124, 190, 205, 235
NheI GCTAGC 1 cut(s) 107
NlaIII CATG 1 cut(s) 286
NsiI ATGCAT 1 cut(s) 442
PagI TCATGA 1 cut(s) 282
PauI GCGCGC 1 cut(s) 479
PcsI WCGNNNNNNNCGW 1 cut(s) 408
PkrI GCNGC 2 cut(s) 228, 349
PleI GAGTC 1 cut(s) 321
PpsI GAGTC 1 cut(s) 321
PsiI TTATAA 1 cut(s) 449
Psp6I CCWGG 2 cut(s) 394, 483
PspEI GGTNACC 1 cut(s) 420
PspGI CCWGG 2 cut(s) 394, 483
PspPI GGNCC 1 cut(s) 179
PsuI RGATCY 3 cut(s) 9, 124, 205
PteI GCGCGC 1 cut(s) 479
RsaI GTAC 4 cut(s) 131, 141, 152, 393
RsaNI GTAC 4 cut(s) 130, 140, 151, 392
SaqAI TTAA 1 cut(s) 87
SatI GCNGC 2 cut(s) 227, 348
Sau3AI GATC 6 cut(s) 9, 79, 124, 190, 205, 235
Sau96I GGNCC 1 cut(s) 179
SchI GAGTC 1 cut(s) 321
ScrFI CCNGG 4 cut(s) 177, 263, 396, 485
SetI ASST 6 cut(s) 75, 113, 220, 382, 411, 422
SfaNI GCATC 2 cut(s) 213, 449
Sse9I AATT 3 cut(s) 115, 255, 427
SsiI CCGC 2 cut(s) 182, 356
SspMI CTAG 2 cut(s) 108, 209
StyD4I CCNGG 4 cut(s) 175, 261, 394, 483
TaaI ACNGT 1 cut(s) 460
TaqI TCGA 4 cut(s) 15, 193, 246, 492
TasI AATT 3 cut(s) 115, 255, 427
TatI WGTACW 3 cut(s) 129, 139, 150
Tru1I TTAA 1 cut(s) 87
Tru9I TTAA 1 cut(s) 87
TscAI CASTG 1 cut(s) 345
TseI GCWGC 2 cut(s) 226, 347
TspDTI ATGAA 2 cut(s) 271, 299
TspGWI ACGGA 1 cut(s) 264
TspRI CASTG 1 cut(s) 345
XapI RAATTY 1 cut(s) 115
XspI CTAG 2 cut(s) 108, 209
Zsp2I ATGCAT 1 cut(s) 442
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.