pycom07g14750

Mitochondrial inner membrane protein

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
pyrus_communis
Chr7
OG0007144
Physical Location & Seq
Reverse (-)
16810469 .. 16813202
2734 bp
Loading structure...
UTR
Exon/CDS
Intron
N/A

Sequence Viewer

Length: 1047 bp
ATGGGACTTGGCTCCTTTTCTTGTCGGTATATGTCTACTGTAATTGGTGATGGGTCTGATAAGATTAATGACACTGGCTATTTTGCTGAGGTTATTGCGGATAAAACATTGGAAGTAGTAACCTCCCAAGCCCCTGCTGTGAGTGAAGTGGCTGTTGCAGCAGCGGATTCTTTCCCCCCAGTTGCTGCCCTGCAGTACTTGATCGATGGTGTCCATTCTTTTACCGGATTGAATTGGTGGGCATCAATAGCTTTAACAACTATTCTGATTCGAGGGGCTACAGTTCCACTTCTTATAGATCAACTCAGGGCCACTACACAACTTAATTTAATGAGGCCGCGCTTAGAGGAGTTAAGACAACAGATGCAAAATATGGCAACAGATCCAAATGTTATGCAAGAGGGTCAGAGACGAATGAAGGCATTGTTCAACGAATATGGAGTCACTCCGTTTTCTCAATTGAAAGGACTGTTCATTCAAGGTCCCATCTTCGTCTGTTTTTTCTTAGCTATTAGAAACATGGCCGAGAAGGTTCCTTCTTTTGAAAGTGGTGGGGCTCTTTGGTTTACTAATCTCGCTACCCCCGATAGTCTTCTCATCCTTCCAGTTCTAACATCATTGACATTTCTGATTACAGTAGAGGAGGGTCTGGAAGGGAATCCTATTGCCCAAACTATGAAAAACTATTCTAGAATCCTTGCTGCCATTTCAATTCCTGTAATGATGAGTTTCCCTAAGGCGCTGTTCTGTTATTGGCTGACCTCCAACATGTTCTCCCTTGCATACGGATTAGTAATTAGGCGCCCAGAGGTGAAGAGTTACCTTCGCCTTCCAGTAATTCCCATGCCACCTCCTACTGCATCCAAGCCTCATTCCTTTATGTCTTCGGCAATTGAACAAGATACTACCGTTGAATCAGAACCTTCGTTATGCTCAGAGCAAACAAAGGCTGCAGACACAAGAATATCACGGACTTCAATTCTCAAGCAGAGACTTAGAATTCTGGAAAAGAAAATCAAGGAAAAAAAGAAGCAAAACAAGATGTGA
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
GO:0005575 GO:0016020
KEGG Pathways
Metabolic & Signaling
ko02024 ko03060 ko03070 map02024 map03060 map03070
Pfam Domains
Protein Families
60KD_IMP

Protein Analysis

349

Amino Acids

38.64

Weight (kDa)

8.95

Isoelectric Point (pI)

46.49

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
60KD_IMP PF02096 79 - 267 1.9e-26 60Kd inner membrane protein
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Publication-ready
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Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AccB1I GGYRCC 1 cut(s) 801
AccI GTMKAC 1 cut(s) 35
AccII CGCG 1 cut(s) 340
AciI CCGC 3 cut(s) 98, 164, 338
AclWI GGATC 1 cut(s) 377
AcoI YGGCCR 1 cut(s) 522
AcsI RAATTY 1 cut(s) 999
AcyI GRCGYC 1 cut(s) 802
AfaI GTAC 1 cut(s) 197
AflIII ACRYGT 1 cut(s) 768
AgsI TTSAA 9 cut(s) 232, 430, 463, 479, 545, 711, 896, 914, 978
AluBI AGCT 2 cut(s) 251, 509
AluI AGCT 2 cut(s) 251, 509
Alw26I GTCTC 2 cut(s) 403, 985
AlwI GGATC 1 cut(s) 377
AlwNI CAGNNNCTG 1 cut(s) 185
AoxI GGCC 3 cut(s) 309, 335, 522
ApeKI GCWGC 5 cut(s) 158, 161, 185, 701, 950
ApoI RAATTY 1 cut(s) 999
ArsI GACNNNNNNTTYG 2 cut(s) 426, 458
AseI ATTAAT 1 cut(s) 66
AspLEI GCGC 3 cut(s) 342, 742, 804
AspS9I GGNCC 2 cut(s) 309, 482
AsuHPI GGTGA 2 cut(s) 59, 823
AvaII GGWCC 1 cut(s) 482
AxyI CCTNAGG 1 cut(s) 735
BaeI ACNNNNGTAYC 2 cut(s) 894, 927
BanI GGYRCC 1 cut(s) 801
BanII GRGCYC 1 cut(s) 559
BbsI GAAGAC 2 cut(s) 584, 876
BbvCI CCTCAGC 1 cut(s) 87
BbvI GCAGC 5 cut(s) 170, 172, 173, 688, 937
BccI CCATC 3 cut(s) 44, 200, 494
BcoDI GTCTC 2 cut(s) 403, 985
BfaI CTAG 1 cut(s) 690
BfmI CTRYAG 3 cut(s) 191, 279, 951
BfoI RGCGCY 2 cut(s) 743, 805
BisI GCNGC 6 cut(s) 159, 162, 186, 338, 702, 951
BlsI GCNGC 6 cut(s) 160, 163, 187, 339, 703, 952
BmcAI AGTACT 1 cut(s) 197
Bme18I GGWCC 1 cut(s) 482
BmgT120I GGNCC 2 cut(s) 309, 482
BmiI GGNNCC 4 cut(s) 13, 484, 534, 803
BmrI ACTGGG 1 cut(s) 173
BmsI GCATC 3 cut(s) 251, 354, 869
BmuI ACTGGG 1 cut(s) 173
BpiI GAAGAC 2 cut(s) 584, 876
Bpu10I CCTNAGC 1 cut(s) 87
BpuEI CTTGAG 1 cut(s) 968
Bsa29I ATCGAT 1 cut(s) 204
BsaHI GRCGYC 1 cut(s) 802
BsaWI WCCGGW 1 cut(s) 224
BsaXI ACNNNNNCTCC 2 cut(s) 758, 788
Bse1I ACTGG 4 cut(s) 79, 179, 605, 833
Bse21I CCTNAGG 1 cut(s) 735
BseCI ATCGAT 1 cut(s) 204
BseGI GGATG 2 cut(s) 597, 860
BseMII CTCAG 3 cut(s) 78, 319, 948
BseNI ACTGG 4 cut(s) 79, 179, 605, 833
BseRI GAGGAG 2 cut(s) 362, 656
BseXI GCAGC 5 cut(s) 170, 172, 173, 688, 937
Bsh1236I CGCG 1 cut(s) 340
BshFI GGCC 3 cut(s) 311, 337, 524
BshNI GGYRCC 1 cut(s) 801
BshVI ATCGAT 1 cut(s) 204
BsiSI CCGG 1 cut(s) 225
BslFI GGGAC 2 cut(s) 18, 468
BsmAI GTCTC 2 cut(s) 403, 985
BsmBI CGTCTC 1 cut(s) 403
BsmFI GGGAC 2 cut(s) 18, 468
BsnI GGCC 3 cut(s) 311, 337, 524
Bsp1286I GDGCHC 1 cut(s) 559
Bsp143I GATC 3 cut(s) 201, 298, 382
BspACI CCGC 3 cut(s) 98, 164, 338
BspANI GGCC 3 cut(s) 311, 337, 524
BspCNI CTCAG 3 cut(s) 79, 318, 947
BspDI ATCGAT 1 cut(s) 204
BspFNI CGCG 1 cut(s) 340
BspLI GGNNCC 4 cut(s) 13, 484, 534, 803
BspMAI CTGCAG 2 cut(s) 195, 955
BspPI GGATC 1 cut(s) 377
BspT107I GGYRCC 1 cut(s) 801
BsrI ACTGG 4 cut(s) 79, 179, 605, 833
BssMI GATC 3 cut(s) 201, 298, 382
BssNI GRCGYC 1 cut(s) 802
Bst4CI ACNGT 5 cut(s) 40, 283, 471, 637, 910
Bst6I CTCTTC 1 cut(s) 809
BstACI GRCGYC 1 cut(s) 802
BstDEI CTNAG 7 cut(s) 87, 305, 343, 505, 735, 934, 995
BstF5I GGATG 2 cut(s) 597, 860
BstFNI CGCG 1 cut(s) 340
BstH2I RGCGCY 2 cut(s) 743, 805
BstHHI GCGC 3 cut(s) 342, 742, 804
BstKTI GATC 3 cut(s) 204, 301, 385
BstMAI GTCTC 2 cut(s) 403, 985
BstMBI GATC 3 cut(s) 201, 298, 382
BstMWI GCNNNNNNNGC 2 cut(s) 158, 248
BstNSI RCATGY 1 cut(s) 772
BstSFI CTRYAG 3 cut(s) 191, 279, 951
BstUI CGCG 1 cut(s) 340
BstV1I GCAGC 5 cut(s) 170, 172, 173, 688, 937
BstV2I GAAGAC 2 cut(s) 584, 876
BstX2I RGATCY 1 cut(s) 382
BstYI RGATCY 1 cut(s) 382
Bsu15I ATCGAT 1 cut(s) 204
Bsu36I CCTNAGG 1 cut(s) 735
BsuRI GGCC 3 cut(s) 311, 337, 524
BsuTUI ATCGAT 1 cut(s) 204
BtsCI GGATG 2 cut(s) 597, 860
BtsIMutI CAGTG 1 cut(s) 72
CaiI CAGNNNCTG 1 cut(s) 185
CfoI GCGC 3 cut(s) 342, 742, 804
Cfr13I GGNCC 2 cut(s) 309, 482
ClaI ATCGAT 1 cut(s) 204
Csp6I GTAC 1 cut(s) 196
CviAII CATG 3 cut(s) 520, 769, 844
CviQI GTAC 1 cut(s) 196
DdeI CTNAG 7 cut(s) 87, 305, 343, 505, 735, 934, 995
DinI GGCGCC 1 cut(s) 803
DpnI GATC 3 cut(s) 203, 300, 384
DpnII GATC 3 cut(s) 201, 298, 382
EaeI YGGCCR 1 cut(s) 522
Eam1104I CTCTTC 1 cut(s) 809
EarI CTCTTC 1 cut(s) 809
Eco24I GRGCYC 1 cut(s) 559
Eco47I GGWCC 1 cut(s) 482
Eco81I CCTNAGG 1 cut(s) 735
EcoO109I RGGNCCY 1 cut(s) 482
EcoRI GAATTC 1 cut(s) 999
EcoT38I GRGCYC 1 cut(s) 559
EgeI GGCGCC 1 cut(s) 803
EheI GGCGCC 1 cut(s) 803
Esp3I CGTCTC 1 cut(s) 403
FaeI CATG 3 cut(s) 523, 772, 847
FaqI GGGAC 2 cut(s) 18, 468
FatI CATG 3 cut(s) 519, 768, 843
FblI GTMKAC 1 cut(s) 35
Fnu4HI GCNGC 6 cut(s) 159, 162, 186, 338, 702, 951
FokI GGATG 2 cut(s) 584, 847
FriOI GRGCYC 1 cut(s) 559
Fsp4HI GCNGC 6 cut(s) 159, 162, 186, 338, 702, 951
FspBI CTAG 1 cut(s) 690
GlaI GCGC 3 cut(s) 341, 741, 803
GluI GCNGC 6 cut(s) 159, 162, 186, 338, 702, 951
HaeII RGCGCY 2 cut(s) 743, 805
HaeIII GGCC 3 cut(s) 311, 337, 524
HapII CCGG 1 cut(s) 225
HhaI GCGC 3 cut(s) 342, 742, 804
Hin1I GRCGYC 1 cut(s) 802
Hin1II CATG 3 cut(s) 523, 772, 847
Hin6I GCGC 3 cut(s) 340, 740, 802
HinP1I GCGC 3 cut(s) 340, 740, 802
HinfI GANTC 6 cut(s) 167, 268, 441, 658, 693, 914
HpaII CCGG 1 cut(s) 225
HphI GGTGA 2 cut(s) 59, 823
Hpy166II GTNNAC 2 cut(s) 36, 567
Hpy188I TCNGA 6 cut(s) 58, 267, 408, 630, 919, 937
Hpy188III TCNNGA 3 cut(s) 650, 690, 1004
Hpy8I GTNNAC 2 cut(s) 36, 567
HpyAV CCTTC 8 cut(s) 412, 523, 546, 611, 647, 833, 839, 933
HpyCH4III ACNGT 5 cut(s) 40, 283, 471, 637, 910
HpyCH4V TGCA 7 cut(s) 158, 193, 367, 397, 782, 860, 953
HpyF10VI GCNNNNNNNGC 2 cut(s) 158, 248
HpyF3I CTNAG 7 cut(s) 87, 305, 343, 505, 735, 934, 995
Hsp92I GRCGYC 1 cut(s) 802
Hsp92II CATG 3 cut(s) 523, 772, 847
HspAI GCGC 3 cut(s) 340, 740, 802
KasI GGCGCC 1 cut(s) 801
Kzo9I GATC 3 cut(s) 201, 298, 382
LmnI GCTCC 1 cut(s) 17
Lsp1109I GCAGC 5 cut(s) 170, 172, 173, 688, 937
LweI GCATC 3 cut(s) 251, 354, 869
MaeI CTAG 1 cut(s) 690
MaeIII GTNAC 3 cut(s) 118, 442, 818
MalI GATC 3 cut(s) 203, 300, 384
MboI GATC 3 cut(s) 201, 298, 382
MboII GAAGA 4 cut(s) 481, 584, 826, 876
MfeI CAATTG 2 cut(s) 458, 891
MflI RGATCY 1 cut(s) 382
MhlI GDGCHC 1 cut(s) 559
Mly113I GGCGCC 1 cut(s) 802
MlyI GAGTC 1 cut(s) 450
MmeI TCCRAC 1 cut(s) 789
MseI TTAA 5 cut(s) 66, 254, 324, 329, 353
MspA1I CMGCKG 1 cut(s) 164
MspI CCGG 1 cut(s) 225
MunI CAATTG 2 cut(s) 458, 891
MvnI CGCG 1 cut(s) 340
MwoI GCNNNNNNNGC 2 cut(s) 158, 248
NarI GGCGCC 1 cut(s) 802
NdeII GATC 3 cut(s) 201, 298, 382
NlaIII CATG 3 cut(s) 523, 772, 847
NlaIV GGNNCC 4 cut(s) 13, 484, 534, 803
NmeAIII GCCGAG 1 cut(s) 550
NmuCI GTSAC 1 cut(s) 442
NspI RCATGY 1 cut(s) 772
PciI ACATGT 1 cut(s) 768
PcsI WCGNNNNNNNCGW 1 cut(s) 582
PfeI GAWTC 5 cut(s) 167, 268, 658, 693, 914
PkrI GCNGC 6 cut(s) 160, 163, 187, 339, 703, 952
PleI GAGTC 1 cut(s) 449
PluTI GGCGCC 1 cut(s) 805
PpsI GAGTC 1 cut(s) 449
PpuMI RGGWCCY 1 cut(s) 482
PscI ACATGT 1 cut(s) 768
PshBI ATTAAT 1 cut(s) 66
Psp5II RGGWCCY 1 cut(s) 482
PspN4I GGNNCC 4 cut(s) 13, 484, 534, 803
PspPI GGNCC 2 cut(s) 309, 482
PspPPI RGGWCCY 1 cut(s) 482
PstI CTGCAG 2 cut(s) 195, 955
PstNI CAGNNNCTG 1 cut(s) 185
PsuI RGATCY 1 cut(s) 382
RsaI GTAC 1 cut(s) 197
RsaNI GTAC 1 cut(s) 196
SaqAI TTAA 5 cut(s) 66, 254, 324, 329, 353
SatI GCNGC 6 cut(s) 159, 162, 186, 338, 702, 951
Sau3AI GATC 3 cut(s) 201, 298, 382
Sau96I GGNCC 2 cut(s) 309, 482
ScaI AGTACT 1 cut(s) 197
SchI GAGTC 1 cut(s) 450
SduI GDGCHC 1 cut(s) 559
SfaNI GCATC 3 cut(s) 251, 354, 869
SfcI CTRYAG 3 cut(s) 191, 279, 951
SfoI GGCGCC 1 cut(s) 803
SinI GGWCC 1 cut(s) 482
SmlI CTYRAG 1 cut(s) 983
SmoI CTYRAG 1 cut(s) 983
SsiI CCGC 3 cut(s) 98, 164, 338
SspDI GGCGCC 1 cut(s) 801
SspMI CTAG 1 cut(s) 690
TaaI ACNGT 5 cut(s) 40, 283, 471, 637, 910
TaqI TCGA 2 cut(s) 204, 271
TatI WGTACW 1 cut(s) 195
TauI GCSGC 1 cut(s) 340
TfiI GAWTC 5 cut(s) 167, 268, 658, 693, 914
Tru1I TTAA 5 cut(s) 66, 254, 324, 329, 353
Tru9I TTAA 5 cut(s) 66, 254, 324, 329, 353
TscAI CASTG 1 cut(s) 79
TseFI GTSAC 1 cut(s) 442
TseI GCWGC 5 cut(s) 158, 161, 185, 701, 950
Tsp45I GTSAC 1 cut(s) 442
TspDTI ATGAA 3 cut(s) 431, 463, 692
TspGWI ACGGA 3 cut(s) 438, 801, 985
TspRI CASTG 1 cut(s) 79
VpaK11BI GGWCC 1 cut(s) 482
VspI ATTAAT 1 cut(s) 66
XapI RAATTY 1 cut(s) 999
XbaI TCTAGA 1 cut(s) 689
XceI RCATGY 1 cut(s) 772
XmiI GTMKAC 1 cut(s) 35
XspI CTAG 1 cut(s) 690
ZrmI AGTACT 1 cut(s) 197
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.