pycom07g18230

TPL-binding domain in jasmonate signalling

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
pyrus_communis
Chr7
OG0002230
Physical Location & Seq
Reverse (-)
20721135 .. 20723181
2047 bp
Loading structure...
UTR
Exon/CDS
Intron
N/A

Sequence Viewer

Length: 1776 bp
ATGGGTTATGACAATTCTTCCAGATTAGATTCAAAGCGTGGTCACCCGTGGTTTATGGATTCCAATGGGCCGGAGCTCTTCAACAACAAGAAGCAAACAATGGAGTCTGTTAATGGCAGGCCAGTTTCAGAGACGGGTTATGACAATTCTTCCAGATTAGATTCAAAGCGAGGTTACCCGTGGTTTGTGGATTCCAATGGGACGGAGCTTTTCAACAACAAGAAGCAAGCAATGGATGCTTTTAATGGCAGGCTAGTTTCAGGAGTTCCACATTTGGCTGTTTTCCCGTGGGAAAACACTTCAGGGTTTCAATCAGTTCCAGGTCAATTCACTGATCGGCTTTTCGGAACTGAGCCAGTACGGACAATCGACCCAGGTGAGAGGAACATTCAATCCGTTGGAAATGGAAATATGAATTTGGGAAGAAAGGGTTTTGAGAATCAATATGGAAATGATTCGTCAATGGGTTTGTCAATGTCTCACTCTATCGAAGACCCCTCATCATGTCTCAGTTTTGGTGGAATCAGAAAAGTGAAGGTCAATGAAGTTAGGGACTCTGAGAATGTAGTGTCTGCATCTATGGGGCACTCCTACATTAGAGGAGACAACACTACAATTTCAGTGGGTACTGCGTATAATAAGAGCAATGAAAACAGCATATCTTTGGGTTCGACTTATAACAATGTTGAAGAGAATGCAATCTCAATAGGGCCTTCCTTTAATAAGGCAGATGAAAATTTCATTTTAATGGGTCACACCTACAGTAAGGAAAATGGGAATTTCACATTGATGGCTCATAATTATAACAAGGGCGACAATGGTGACTTATCAATTGGACGGCCCTTTGACAAGGAGGATGGAAGATTTATATCAATAGGCCAGTCATATGAAAAGGGAGATAGCAGTTTCATTTCGTTAGGTAGCTCTTACAACAAAGGTCATGAGAGTTTTGTTTCTAGTGGTGCAACATATAGTAAAGCAAACGAGAATTTCATCTCAATGCCTCCGACATATAACAAGGCAGATTTGAATGCTGTACCAACTGGTCCTCCCTATGATAAAGGAGATAGCAGTTCTATGAGTCATAATTTCAATAAGAGTGAGAGCACCACCATCGCTTTTGGAAGTTTTCATGATGAACTCGACACAAATCCTTCTGGCAGGATCATCAACAGCTATGATTTGCTGACGAGTAGCCAGAACACAGCTCAACAGTCAGAAGAACCTGGCCTGAAGGATCCAGTTCGGTCACATACGGATCCAAATGTAGGTGAAGCTCTGCAAGTTGATTCTAAAACTGATATGGTTCCAAAAATTAAAGAGCCAAGAACTGCTAGGAAAGCTCCTCCAAACAACTTCCCTTCAAATGTCAAAAGTTTGTTATCAACTGGTATGTTTGATGGTGTTCTGGTGAAGTATGTTTCCTGGTCACGGGAGAAAAACCTCAAAGCAACAATAAAAGGGGCCGGGTATCTTTGTTCCTGTGATCAATGCAATCACCCAAAGTCTCTTAATGCTTATGAGTTCGAGCGTCATGCTGGTGCCAAGACCAAACATCCCAATAATCACATCTACTTTGAGAATGGAAAGACCATTTATGCAGTAGTTCAAGAGCTGAAGAACACACCTCAGGACGTGCTGTTTGACGCCATTCAAACTGTAACAGGCTCTCCTATTAATCAGAAGAATTTCCGCATCTGGAAAGCATCATATCAAGCCGCAACCCGTGAACTTCAGCGTATCTATGGAAAAGATGAAGCCGCTGCACCTTCTTGA
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
No pathways identified.
Pfam Domains
Protein Families
Jas

Protein Analysis

592

Amino Acids

65.1

Weight (kDa)

6.3

Isoelectric Point (pI)

39.25

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
TDBD PF16135 485 - 538 3.8e-13 Tify domain binding domain
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Publication-ready
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Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AanI TTATAA 2 cut(s) 678, 804
AccB1I GGYRCC 1 cut(s) 1541
AciI CCGC 3 cut(s) 1693, 1719, 1761
AclWI GGATC 5 cut(s) 1172, 1232, 1245, 1253, 1266
AcsI RAATTY 5 cut(s) 415, 736, 778, 988, 1687
AcuI CTGAAG 4 cut(s) 285, 1253, 1637, 1718
AcyI GRCGYC 1 cut(s) 1647
AfaI GTAC 3 cut(s) 360, 628, 1038
AfiI CCNNNNNNNGG 2 cut(s) 1268, 1431
AjiI CACGTC 1 cut(s) 1636
AjnI CCWGG 4 cut(s) 319, 373, 1225, 1424
AloI GAACNNNNNNTCC 2 cut(s) 377, 409
AluBI AGCT 8 cut(s) 76, 208, 924, 1176, 1208, 1277, 1343, 1615
AluI AGCT 8 cut(s) 76, 208, 924, 1176, 1208, 1277, 1343, 1615
Alw21I GWGCWC 2 cut(s) 78, 1109
Alw26I GTCTC 5 cut(s) 125, 483, 512, 597, 1512
AlwI GGATC 5 cut(s) 1172, 1232, 1245, 1253, 1266
AoxI GGCC 7 cut(s) 68, 119, 710, 839, 877, 1228, 1464
ApeKI GCWGC 1 cut(s) 1763
ApoI RAATTY 5 cut(s) 415, 736, 778, 988, 1687
ArsI GACNNNNNNTTYG 2 cut(s) 1625, 1657
AseI ATTAAT 1 cut(s) 1677
Asp700I GAANNNNTTC 2 cut(s) 454, 1688
AspS9I GGNCC 5 cut(s) 68, 710, 840, 1046, 1464
AsuC2I CCSGG 1 cut(s) 1468
AsuHPI GGTGA 6 cut(s) 35, 389, 833, 1283, 1423, 1490
AvaII GGWCC 1 cut(s) 1046
AxyI CCTNAGG 1 cut(s) 1629
BaeGI GKGCMC 1 cut(s) 588
BamHI GGATCC 2 cut(s) 1237, 1258
BanI GGYRCC 1 cut(s) 1541
BanII GRGCYC 1 cut(s) 78
BbsI GAAGAC 1 cut(s) 498
Bbv12I GWGCWC 2 cut(s) 78, 1109
BbvI GCAGC 1 cut(s) 1750
BccI CCATC 4 cut(s) 784, 851, 1121, 1394
BceAI ACGGC 1 cut(s) 854
BcgI CGANNNNNNTGC 4 cut(s) 1096, 1130, 1517, 1551
BciT130I CCWGG 4 cut(s) 321, 375, 1227, 1426
BclI TGATCA 1 cut(s) 1486
BcnI CCSGG 1 cut(s) 1468
BcoDI GTCTC 5 cut(s) 125, 483, 512, 597, 1512
BfaI CTAG 3 cut(s) 254, 957, 1335
BfmI CTRYAG 1 cut(s) 760
BisI GCNGC 3 cut(s) 1719, 1761, 1764
BlsI GCNGC 3 cut(s) 1720, 1762, 1765
Bme1390I CCNGG 5 cut(s) 321, 375, 1227, 1426, 1468
Bme18I GGWCC 1 cut(s) 1046
BmgBI CACGTC 1 cut(s) 1636
BmgT120I GGNCC 5 cut(s) 68, 710, 840, 1046, 1464
BmiI GGNNCC 5 cut(s) 1239, 1260, 1308, 1465, 1543
BmrFI CCNGG 5 cut(s) 321, 375, 1227, 1426, 1468
BmsI GCATC 4 cut(s) 226, 584, 1704, 1715
BpiI GAAGAC 1 cut(s) 498
BpuMI CCSGG 1 cut(s) 1468
BsaBI GATNNNNATC 1 cut(s) 868
BsaHI GRCGYC 1 cut(s) 1647
BsaJI CCNNGG 4 cut(s) 47, 179, 287, 373
BsaXI ACNNNNNCTCC 4 cut(s) 1033, 1063, 1654, 1684
Bsc4I CCNNNNNNNGG 2 cut(s) 1268, 1431
Bse1I ACTGG 6 cut(s) 122, 356, 880, 1048, 1241, 1393
Bse21I CCTNAGG 1 cut(s) 1629
Bse3DI GCAATG 2 cut(s) 237, 652
Bse8I GATNNNNATC 1 cut(s) 868
BseBI CCWGG 4 cut(s) 321, 375, 1227, 1426
BseDI CCNNGG 4 cut(s) 47, 179, 287, 373
BseGI GGATG 3 cut(s) 241, 862, 1555
BseJI GATNNNNATC 1 cut(s) 868
BseLI CCNNNNNNNGG 2 cut(s) 1268, 1431
BseMI GCAATG 2 cut(s) 237, 652
BseMII CTCAG 4 cut(s) 342, 523, 549, 1643
BseNI ACTGG 6 cut(s) 122, 356, 880, 1048, 1241, 1393
BseRI GAGGAG 2 cut(s) 615, 1335
BseSI GKGCMC 1 cut(s) 588
BseXI GCAGC 1 cut(s) 1750
BsgI GTGCAG 1 cut(s) 1749
BshFI GGCC 7 cut(s) 70, 121, 712, 841, 879, 1230, 1466
BshNI GGYRCC 1 cut(s) 1541
BsiHKAI GWGCWC 2 cut(s) 78, 1109
BsiSI CCGG 2 cut(s) 71, 1467
BslFI GGGAC 2 cut(s) 214, 566
BslI CCNNNNNNNGG 2 cut(s) 1268, 1431
BsmAI GTCTC 5 cut(s) 125, 483, 512, 597, 1512
BsmBI CGTCTC 1 cut(s) 125
BsmFI GGGAC 2 cut(s) 214, 566
BsmI GAATGC 2 cut(s) 700, 1036
BsnI GGCC 7 cut(s) 70, 121, 712, 841, 879, 1230, 1466
Bsp1286I GDGCHC 3 cut(s) 78, 588, 1109
Bsp143I GATC 5 cut(s) 334, 1164, 1237, 1258, 1486
BspACI CCGC 3 cut(s) 1693, 1719, 1761
BspANI GGCC 7 cut(s) 70, 121, 712, 841, 879, 1230, 1466
BspCNI CTCAG 4 cut(s) 343, 522, 550, 1642
BspHI TCATGA 2 cut(s) 940, 1132
BspLI GGNNCC 5 cut(s) 1239, 1260, 1308, 1465, 1543
BspPI GGATC 5 cut(s) 1172, 1232, 1245, 1253, 1266
BspQI GCTCTTC 1 cut(s) 83
BspT107I GGYRCC 1 cut(s) 1541
BsrDI GCAATG 2 cut(s) 237, 652
BsrI ACTGG 6 cut(s) 122, 356, 880, 1048, 1241, 1393
BssECI CCNNGG 4 cut(s) 47, 179, 287, 373
BssMI GATC 5 cut(s) 334, 1164, 1237, 1258, 1486
BssNI GRCGYC 1 cut(s) 1647
Bst2UI CCWGG 4 cut(s) 321, 375, 1227, 1426
Bst4CI ACNGT 3 cut(s) 764, 1215, 1660
Bst6I CTCTTC 2 cut(s) 83, 684
BstACI GRCGYC 1 cut(s) 1647
BstAPI GCANNNNNTGC 1 cut(s) 236
BstC8I GCNNGC 3 cut(s) 119, 228, 251
BstDEI CTNAG 4 cut(s) 351, 509, 558, 1629
BstDSI CCRYGG 3 cut(s) 47, 179, 287
BstEII GGTNACC 2 cut(s) 41, 173
BstF5I GGATG 3 cut(s) 241, 862, 1555
BstKTI GATC 5 cut(s) 337, 1167, 1240, 1261, 1489
BstMAI GTCTC 5 cut(s) 125, 483, 512, 597, 1512
BstMBI GATC 5 cut(s) 334, 1164, 1237, 1258, 1486
BstMWI GCNNNNNNNGC 2 cut(s) 236, 1340
BstNI CCWGG 4 cut(s) 321, 375, 1227, 1426
BstPI GGTNACC 2 cut(s) 41, 173
BstSCI CCNGG 5 cut(s) 319, 373, 1225, 1424, 1466
BstSFI CTRYAG 1 cut(s) 760
BstSLI GKGCMC 1 cut(s) 588
BstV1I GCAGC 1 cut(s) 1750
BstV2I GAAGAC 1 cut(s) 498
BstX2I RGATCY 2 cut(s) 1237, 1258
BstYI RGATCY 2 cut(s) 1237, 1258
Bsu36I CCTNAGG 1 cut(s) 1629
BsuRI GGCC 7 cut(s) 70, 121, 712, 841, 879, 1230, 1466
BtgI CCRYGG 3 cut(s) 47, 179, 287
BtgZI GCGATG 1 cut(s) 1099
BtrI CACGTC 1 cut(s) 1636
BtsCI GGATG 3 cut(s) 241, 862, 1555
BtsIMutI CAGTG 2 cut(s) 330, 627
Cac8I GCNNGC 3 cut(s) 119, 228, 251
CciI TCATGA 2 cut(s) 940, 1132
Cfr13I GGNCC 5 cut(s) 68, 710, 840, 1046, 1464
CseI GACGC 2 cut(s) 1520, 1655
Csp6I GTAC 3 cut(s) 359, 627, 1037
CspCI CAANNNNNGTGG 2 cut(s) 603, 638
CviAII CATG 4 cut(s) 504, 941, 1133, 1535
CviQI GTAC 3 cut(s) 359, 627, 1037
DdeI CTNAG 4 cut(s) 351, 509, 558, 1629
DpnI GATC 5 cut(s) 336, 1166, 1239, 1260, 1488
DpnII GATC 5 cut(s) 334, 1164, 1237, 1258, 1486
Eam1104I CTCTTC 2 cut(s) 83, 684
EarI CTCTTC 2 cut(s) 83, 684
Ecl136II GAGCTC 1 cut(s) 76
Eco24I GRGCYC 1 cut(s) 78
Eco47I GGWCC 1 cut(s) 1046
Eco53kI GAGCTC 1 cut(s) 76
Eco57I CTGAAG 4 cut(s) 285, 1253, 1637, 1718
Eco81I CCTNAGG 1 cut(s) 1629
Eco91I GGTNACC 2 cut(s) 41, 173
EcoICRI GAGCTC 1 cut(s) 76
EcoO109I RGGNCCY 1 cut(s) 710
EcoO65I GGTNACC 2 cut(s) 41, 173
EcoRII CCWGG 4 cut(s) 319, 373, 1225, 1424
EcoT38I GRGCYC 1 cut(s) 78
Esp3I CGTCTC 1 cut(s) 125
FaeI CATG 4 cut(s) 507, 944, 1136, 1538
FaqI GGGAC 2 cut(s) 214, 566
FatI CATG 4 cut(s) 503, 940, 1132, 1534
FauNDI CATATG 1 cut(s) 886
FbaI TGATCA 1 cut(s) 1486
Fnu4HI GCNGC 3 cut(s) 1719, 1761, 1764
FokI GGATG 3 cut(s) 248, 869, 1542
FriOI GRGCYC 1 cut(s) 78
Fsp4HI GCNGC 3 cut(s) 1719, 1761, 1764
FspBI CTAG 3 cut(s) 254, 957, 1335
GluI GCNGC 3 cut(s) 1719, 1761, 1764
HaeIII GGCC 7 cut(s) 70, 121, 712, 841, 879, 1230, 1466
HapII CCGG 2 cut(s) 71, 1467
HgaI GACGC 2 cut(s) 1520, 1655
Hin1I GRCGYC 1 cut(s) 1647
Hin1II CATG 4 cut(s) 507, 944, 1136, 1538
HpaII CCGG 2 cut(s) 71, 1467
HphI GGTGA 6 cut(s) 35, 389, 833, 1283, 1423, 1490
Hpy166II GTNNAC 1 cut(s) 1730
Hpy188I TCNGA 7 cut(s) 130, 347, 527, 559, 1008, 1219, 1683
Hpy188III TCNNGA 9 cut(s) 21, 153, 261, 941, 1133, 1610, 1631, 1699, 1773
Hpy8I GTNNAC 1 cut(s) 1730
HpyAV CCTTC 5 cut(s) 529, 723, 1164, 1228, 1371
HpyCH4III ACNGT 3 cut(s) 764, 1215, 1660
HpyCH4IV ACGT 1 cut(s) 1635
HpyCH4V TGCA 7 cut(s) 575, 698, 965, 1282, 1494, 1601, 1766
HpyF10VI GCNNNNNNNGC 2 cut(s) 236, 1340
HpyF3I CTNAG 4 cut(s) 351, 509, 558, 1629
HpySE526I ACGT 1 cut(s) 1635
Hsp92I GRCGYC 1 cut(s) 1647
Hsp92II CATG 4 cut(s) 507, 944, 1136, 1538
Ksp22I TGATCA 1 cut(s) 1486
Kzo9I GATC 5 cut(s) 334, 1164, 1237, 1258, 1486
LguI GCTCTTC 1 cut(s) 83
LmnI GCTCC 3 cut(s) 73, 205, 1348
Lsp1109I GCAGC 1 cut(s) 1750
LweI GCATC 4 cut(s) 226, 584, 1704, 1715
MaeI CTAG 3 cut(s) 254, 957, 1335
MaeII ACGT 1 cut(s) 1635
MaeIII GTNAC 7 cut(s) 41, 173, 752, 821, 1248, 1428, 1660
MalI GATC 5 cut(s) 336, 1166, 1239, 1260, 1488
MboI GATC 5 cut(s) 334, 1164, 1237, 1258, 1486
MfeI CAATTG 1 cut(s) 831
MflI RGATCY 2 cut(s) 1237, 1258
MhlI GDGCHC 3 cut(s) 78, 588, 1109
MlyI GAGTC 3 cut(s) 113, 548, 1090
MmeI TCCRAC 2 cut(s) 379, 1031
MroXI GAANNNNTTC 2 cut(s) 454, 1688
MseI TTAA 7 cut(s) 111, 243, 720, 746, 1317, 1512, 1677
MslI CAYNNNNRTG 4 cut(s) 746, 788, 998, 1539
MspA1I CMGCKG 1 cut(s) 1763
MspI CCGG 2 cut(s) 71, 1467
MspR9I CCNGG 5 cut(s) 321, 375, 1227, 1426, 1468
MunI CAATTG 1 cut(s) 831
Mva1269I GAATGC 2 cut(s) 700, 1036
MvaI CCWGG 4 cut(s) 321, 375, 1227, 1426
MwoI GCNNNNNNNGC 2 cut(s) 236, 1340
NciI CCSGG 1 cut(s) 1468
NdeI CATATG 1 cut(s) 886
NdeII GATC 5 cut(s) 334, 1164, 1237, 1258, 1486
NlaIII CATG 4 cut(s) 507, 944, 1136, 1538
NlaIV GGNNCC 5 cut(s) 1239, 1260, 1308, 1465, 1543
NmuCI GTSAC 5 cut(s) 41, 752, 821, 1248, 1428
PagI TCATGA 2 cut(s) 940, 1132
PciSI GCTCTTC 1 cut(s) 83
PctI GAATGC 2 cut(s) 700, 1036
PdmI GAANNNNTTC 2 cut(s) 454, 1688
PfeI GAWTC 8 cut(s) 29, 59, 161, 191, 439, 455, 522, 1289
PkrI GCNGC 3 cut(s) 1720, 1762, 1765
PleI GAGTC 3 cut(s) 112, 548, 1089
PpsI GAGTC 3 cut(s) 112, 548, 1089
PshBI ATTAAT 1 cut(s) 1677
PsiI TTATAA 2 cut(s) 678, 804
Psp124BI GAGCTC 1 cut(s) 78
Psp6I CCWGG 4 cut(s) 319, 373, 1225, 1424
PspEI GGTNACC 2 cut(s) 41, 173
PspGI CCWGG 4 cut(s) 319, 373, 1225, 1424
PspN4I GGNNCC 5 cut(s) 1239, 1260, 1308, 1465, 1543
PspPI GGNCC 5 cut(s) 68, 710, 840, 1046, 1464
PsuI RGATCY 2 cut(s) 1237, 1258
RsaI GTAC 3 cut(s) 360, 628, 1038
RsaNI GTAC 3 cut(s) 359, 627, 1037
RseI CAYNNNNRTG 4 cut(s) 746, 788, 998, 1539
SacI GAGCTC 1 cut(s) 78
SapI GCTCTTC 1 cut(s) 83
SaqAI TTAA 7 cut(s) 111, 243, 720, 746, 1317, 1512, 1677
SatI GCNGC 3 cut(s) 1719, 1761, 1764
Sau3AI GATC 5 cut(s) 334, 1164, 1237, 1258, 1486
Sau96I GGNCC 5 cut(s) 68, 710, 840, 1046, 1464
SchI GAGTC 3 cut(s) 113, 548, 1090
ScrFI CCNGG 5 cut(s) 321, 375, 1227, 1426, 1468
SduI GDGCHC 3 cut(s) 78, 588, 1109
SfaNI GCATC 4 cut(s) 226, 584, 1704, 1715
SfcI CTRYAG 1 cut(s) 760
SinI GGWCC 1 cut(s) 1046
SmiMI CAYNNNNRTG 4 cut(s) 746, 788, 998, 1539
SsiI CCGC 3 cut(s) 1693, 1719, 1761
SspMI CTAG 3 cut(s) 254, 957, 1335
SstI GAGCTC 1 cut(s) 78
StyD4I CCNGG 5 cut(s) 319, 373, 1225, 1424, 1466
TaaI ACNGT 3 cut(s) 764, 1215, 1660
TaiI ACGT 1 cut(s) 1638
TaqI TCGA 5 cut(s) 369, 489, 671, 1143, 1527
TaqII GACCGA 1 cut(s) 1236
TauI GCSGC 2 cut(s) 1721, 1763
TfiI GAWTC 8 cut(s) 29, 59, 161, 191, 439, 455, 522, 1289
Tru1I TTAA 7 cut(s) 111, 243, 720, 746, 1317, 1512, 1677
Tru9I TTAA 7 cut(s) 111, 243, 720, 746, 1317, 1512, 1677
TscAI CASTG 2 cut(s) 337, 627
TseFI GTSAC 5 cut(s) 41, 752, 821, 1248, 1428
TseI GCWGC 1 cut(s) 1763
Tsp45I GTSAC 5 cut(s) 41, 752, 821, 1248, 1428
TspGWI ACGGA 4 cut(s) 218, 376, 385, 1271
TspRI CASTG 2 cut(s) 337, 627
VpaK11BI GGWCC 1 cut(s) 1046
VspI ATTAAT 1 cut(s) 1677
XapI RAATTY 5 cut(s) 415, 736, 778, 988, 1687
XmnI GAANNNNTTC 2 cut(s) 454, 1688
XspI CTAG 3 cut(s) 254, 957, 1335
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.