pycom07g20700

38.1 kDa protein-like

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
pyrus_communis
Chr7
OG0006546
Physical Location & Seq
Forward (+)
22687483 .. 22689425
1943 bp
Loading structure...
UTR
Exon/CDS
Intron
N/A

Sequence Viewer

Length: 1029 bp
ATGGGGTTGTTGGGAAGCTTCATAAGGTTCCTGTTGGGGCAATGGTGCAAACCCACCACCACCGGAGGGGACTACTCTGAATCACTTGGCCCTCACGGCGTGTCCGCCGCCACTGTCGGCGTTTCGGCTCTTGCCCAAGACCTCTACAACTTCGAGATCGACCTCTCAGTTTGGGTCCCAGAAGGGCTCGCTCAGCATGTGGTCTCGTCCAGGAAGGCTCAAGCTAACTGGTATAGAAAACTAGTTGTCGCATGGAGGGAAGCCAAACCGCCACCAAGAACACCCGAAGAAGCGGCTAGGCTTGTTATACAAACCCTGAAACAACACCAAAGGCCGGATGTTGAGGGTTTATTGGCTTTCTACGGCCTTCCTCTTCCACATACTCTGGTCAGTCTTTCTGCTGAGGTCCCAACTTCGTTGCCTCAAGGAGTAAAGTACGAGTTCCGGACGCTGCCGGTTGATGGAAAAGCGATTCAAGATGGCGATTCGATGACAGTCTACGTCAGCACAGAGGATCCCAGGGAGTCGTCCTTTGTTCCGATCGATGTGCAAAGAGCTGCCATTCAAAGATCGAAAGCTCGCGCCGTGAGGGACTATACAAAGGCAGATGCACTTAATCAGAAAATCAAAGATGCTGGATACGGGGTACTGAATATTCAAAACGAGGAGATCCTTGCCCGAAAGTATAGGATCCGTCTGAGGGGTATAGATGCACCCGAGGGTGAAATGCCCTATGGAGAAGAAGCCAAACAGGAGCTGGTTAAGCTTATTCAGGGCAAGCGTTTGAGGGTTCTTATCTATGGGGAAGATCGTTACGGGCGCTGTGTAGGTGACGTATACTGCAACGACATATTTGTACAGGAAGTAATGCTCAAGAAGGGGTGTGCATGGCATTACGCAGCCTACGACCAACGCCCAGAATTTGCAACATGGGAAAAAAAGGCTCGGGCGAAGCGAGTTGGGTTGTGGGCTTCATCAAACCCTGAGAAGCCATGGGATTGGAGAAGGGATCGGCGTGAAGGCAAATGA

Protein Analysis

343

Amino Acids

38.66

Weight (kDa)

9.06

Isoelectric Point (pI)

36.43

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
SNase PF00565 230 - 324 2.9e-26 Staphylococcal nuclease homologue
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

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Publication-ready
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Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AccI GTMKAC 2 cut(s) 498, 837
AccII CGCG 1 cut(s) 582
AccIII TCCGGA 1 cut(s) 444
AciI CCGC 4 cut(s) 105, 108, 269, 293
AclWI GGATC 6 cut(s) 509, 522, 664, 685, 698, 1017
AcsI RAATTY 1 cut(s) 920
AdeI CACNNNGTG 1 cut(s) 100
AfaI GTAC 3 cut(s) 437, 648, 858
AfiI CCNNNNNNNGG 4 cut(s) 66, 334, 461, 700
AgsI TTSAA 3 cut(s) 476, 566, 659
AhlI ACTAGT 1 cut(s) 241
AjnI CCWGG 2 cut(s) 209, 518
AluBI AGCT 6 cut(s) 18, 224, 557, 578, 757, 766
AluI AGCT 6 cut(s) 18, 224, 557, 578, 757, 766
Alw26I GTCTC 1 cut(s) 208
AlwI GGATC 6 cut(s) 509, 522, 664, 685, 698, 1017
AlwNI CAGNNNCTG 1 cut(s) 757
Ama87I CYCGRG 2 cut(s) 716, 945
Aor13HI TCCGGA 1 cut(s) 444
AoxI GGCC 3 cut(s) 88, 332, 364
ApeKI GCWGC 3 cut(s) 451, 557, 899
ApoI RAATTY 1 cut(s) 920
AspLEI GCGC 2 cut(s) 584, 822
AspS9I GGNCC 3 cut(s) 89, 175, 406
AsuHPI GGTGA 2 cut(s) 734, 842
AvaI CYCGRG 2 cut(s) 716, 945
AvaII GGWCC 2 cut(s) 175, 406
BamHI GGATCC 2 cut(s) 514, 690
BanII GRGCYC 1 cut(s) 189
BbvCI CCTCAGC 1 cut(s) 402
BbvI GCAGC 3 cut(s) 438, 544, 911
BccI CCATC 2 cut(s) 455, 473
BceAI ACGGC 3 cut(s) 112, 379, 569
BcgI CGANNNNNNTGC 2 cut(s) 529, 563
BciT130I CCWGG 2 cut(s) 211, 520
BciVI GTATCC 1 cut(s) 632
BcoDI GTCTC 1 cut(s) 208
BcuI ACTAGT 1 cut(s) 241
BfaI CTAG 2 cut(s) 242, 297
BfoI RGCGCY 1 cut(s) 823
BfuI GTATCC 1 cut(s) 632
BglI GCCNNNNNGGC 1 cut(s) 96
BisI GCNGC 5 cut(s) 108, 294, 452, 558, 900
BlpI GCTNAGC 1 cut(s) 192
BlsI GCNGC 5 cut(s) 109, 295, 453, 559, 901
Bme1390I CCNGG 2 cut(s) 211, 520
Bme18I GGWCC 2 cut(s) 175, 406
BmeT110I CYCGRG 2 cut(s) 716, 945
BmgT120I GGNCC 3 cut(s) 89, 175, 406
BmiI GGNNCC 6 cut(s) 29, 176, 177, 408, 516, 692
BmrFI CCNGG 2 cut(s) 211, 520
BmsI GCATC 3 cut(s) 598, 622, 700
Bpu10I CCTNAGC 1 cut(s) 402
Bpu1102I GCTNAGC 1 cut(s) 192
BpuEI CTTGAG 3 cut(s) 204, 408, 857
Bsa29I ATCGAT 1 cut(s) 543
BsaI GGTCTC 1 cut(s) 208
BsaJI CCNNGG 4 cut(s) 518, 519, 717, 992
BsaWI WCCGGW 2 cut(s) 62, 444
Bsc4I CCNNNNNNNGG 4 cut(s) 66, 334, 461, 700
Bse118I RCCGGY 1 cut(s) 454
Bse1I ACTGG 1 cut(s) 233
Bse3DI GCAATG 1 cut(s) 47
BseAI TCCGGA 1 cut(s) 444
BseBI CCWGG 2 cut(s) 211, 520
BseCI ATCGAT 1 cut(s) 543
BseDI CCNNGG 4 cut(s) 518, 519, 717, 992
BseGI GGATG 1 cut(s) 343
BseLI CCNNNNNNNGG 4 cut(s) 66, 334, 461, 700
BseMI GCAATG 1 cut(s) 47
BseMII CTCAG 5 cut(s) 180, 206, 393, 689, 975
BseNI ACTGG 1 cut(s) 233
BseRI GAGGAG 1 cut(s) 680
BseXI GCAGC 3 cut(s) 438, 544, 911
Bsh1236I CGCG 1 cut(s) 582
Bsh1285I CGRYCG 1 cut(s) 543
BshFI GGCC 3 cut(s) 90, 334, 366
BshVI ATCGAT 1 cut(s) 543
BsiEI CGRYCG 1 cut(s) 543
BsiHKCI CYCGRG 2 cut(s) 716, 945
BsiSI CCGG 4 cut(s) 63, 335, 445, 455
BslFI GGGAC 4 cut(s) 83, 161, 392, 605
BslI CCNNNNNNNGG 4 cut(s) 66, 334, 461, 700
BsmAI GTCTC 1 cut(s) 208
BsmFI GGGAC 4 cut(s) 83, 161, 392, 605
BsnI GGCC 3 cut(s) 90, 334, 366
Bso31I GGTCTC 1 cut(s) 208
BsoBI CYCGRG 2 cut(s) 716, 945
Bsp1286I GDGCHC 1 cut(s) 189
Bsp13I TCCGGA 1 cut(s) 444
Bsp1407I TGTACA 1 cut(s) 856
Bsp143I GATC 8 cut(s) 156, 514, 540, 569, 669, 690, 808, 1009
Bsp1720I GCTNAGC 1 cut(s) 192
Bsp19I CCATGG 1 cut(s) 992
BspACI CCGC 4 cut(s) 105, 108, 269, 293
BspANI GGCC 3 cut(s) 90, 334, 366
BspCNI CTCAG 5 cut(s) 179, 205, 394, 690, 976
BspDI ATCGAT 1 cut(s) 543
BspEI TCCGGA 1 cut(s) 444
BspFNI CGCG 1 cut(s) 582
BspLI GGNNCC 6 cut(s) 29, 176, 177, 408, 516, 692
BspPI GGATC 6 cut(s) 509, 522, 664, 685, 698, 1017
BspTNI GGTCTC 1 cut(s) 208
BsrDI GCAATG 1 cut(s) 47
BsrFI RCCGGY 1 cut(s) 454
BsrGI TGTACA 1 cut(s) 856
BsrI ACTGG 1 cut(s) 233
BssAI RCCGGY 1 cut(s) 454
BssECI CCNNGG 4 cut(s) 518, 519, 717, 992
BssMI GATC 8 cut(s) 156, 514, 540, 569, 669, 690, 808, 1009
BssNAI GTATAC 1 cut(s) 838
BssT1I CCWWGG 1 cut(s) 992
Bst1107I GTATAC 1 cut(s) 838
Bst2UI CCWGG 2 cut(s) 211, 520
Bst4CI ACNGT 2 cut(s) 115, 496
Bst6I CTCTTC 1 cut(s) 378
BstAUI TGTACA 1 cut(s) 856
BstC8I GCNNGC 3 cut(s) 189, 580, 779
BstDEI CTNAG 5 cut(s) 166, 192, 402, 698, 984
BstDSI CCRYGG 1 cut(s) 992
BstF5I GGATG 1 cut(s) 343
BstFNI CGCG 1 cut(s) 582
BstH2I RGCGCY 1 cut(s) 823
BstHHI GCGC 2 cut(s) 584, 822
BstKTI GATC 8 cut(s) 159, 517, 543, 572, 672, 693, 811, 1012
BstMAI GTCTC 1 cut(s) 208
BstMBI GATC 8 cut(s) 156, 514, 540, 569, 669, 690, 808, 1009
BstMCI CGRYCG 1 cut(s) 543
BstMWI GCNNNNNNNGC 3 cut(s) 96, 193, 763
BstNI CCWGG 2 cut(s) 211, 520
BstNSI RCATGY 1 cut(s) 200
BstSCI CCNGG 2 cut(s) 209, 518
BstUI CGCG 1 cut(s) 582
BstV1I GCAGC 3 cut(s) 438, 544, 911
BstX2I RGATCY 3 cut(s) 514, 669, 690
BstXI CCANNNNNNTGG 1 cut(s) 999
BstYI RGATCY 3 cut(s) 514, 669, 690
BstZ17I GTATAC 1 cut(s) 838
Bsu15I ATCGAT 1 cut(s) 543
BsuI GTATCC 1 cut(s) 632
BsuRI GGCC 3 cut(s) 90, 334, 366
BsuTUI ATCGAT 1 cut(s) 543
BtgI CCRYGG 1 cut(s) 992
BtsCI GGATG 1 cut(s) 343
BtsIMutI CAGTG 1 cut(s) 111
Cac8I GCNNGC 3 cut(s) 189, 580, 779
CaiI CAGNNNCTG 1 cut(s) 757
CfoI GCGC 2 cut(s) 584, 822
Cfr10I RCCGGY 1 cut(s) 454
Cfr13I GGNCC 3 cut(s) 89, 175, 406
ClaI ATCGAT 1 cut(s) 543
CseI GACGC 1 cut(s) 457
Csp6I GTAC 3 cut(s) 436, 647, 857
CviAII CATG 5 cut(s) 197, 252, 888, 930, 993
CviQI GTAC 3 cut(s) 436, 647, 857
DdeI CTNAG 5 cut(s) 166, 192, 402, 698, 984
DpnI GATC 8 cut(s) 158, 516, 542, 571, 671, 692, 810, 1011
DpnII GATC 8 cut(s) 156, 514, 540, 569, 669, 690, 808, 1009
DraIII CACNNNGTG 1 cut(s) 100
Eam1104I CTCTTC 1 cut(s) 378
EarI CTCTTC 1 cut(s) 378
EciI GGCGGA 1 cut(s) 94
Eco130I CCWWGG 1 cut(s) 992
Eco24I GRGCYC 1 cut(s) 189
Eco31I GGTCTC 1 cut(s) 208
Eco47I GGWCC 2 cut(s) 175, 406
Eco88I CYCGRG 2 cut(s) 716, 945
EcoO109I RGGNCCY 2 cut(s) 175, 406
EcoRII CCWGG 2 cut(s) 209, 518
EcoT14I CCWWGG 1 cut(s) 992
EcoT38I GRGCYC 1 cut(s) 189
ErhI CCWWGG 1 cut(s) 992
FaeI CATG 5 cut(s) 200, 255, 891, 933, 996
FaqI GGGAC 4 cut(s) 83, 161, 392, 605
FatI CATG 5 cut(s) 196, 251, 887, 929, 992
FblI GTMKAC 2 cut(s) 498, 837
Fnu4HI GCNGC 5 cut(s) 108, 294, 452, 558, 900
FokI GGATG 1 cut(s) 350
FriOI GRGCYC 1 cut(s) 189
Fsp4HI GCNGC 5 cut(s) 108, 294, 452, 558, 900
FspBI CTAG 2 cut(s) 242, 297
GlaI GCGC 2 cut(s) 583, 821
GluI GCNGC 5 cut(s) 108, 294, 452, 558, 900
HaeII RGCGCY 1 cut(s) 823
HaeIII GGCC 3 cut(s) 90, 334, 366
HapII CCGG 4 cut(s) 63, 335, 445, 455
HgaI GACGC 1 cut(s) 457
HhaI GCGC 2 cut(s) 584, 822
Hin1II CATG 5 cut(s) 200, 255, 891, 933, 996
Hin6I GCGC 2 cut(s) 582, 820
HinP1I GCGC 2 cut(s) 582, 820
HindIII AAGCTT 2 cut(s) 16, 764
HinfI GANTC 4 cut(s) 80, 472, 485, 524
HpaII CCGG 4 cut(s) 63, 335, 445, 455
HphI GGTGA 2 cut(s) 734, 842
Hpy166II GTNNAC 2 cut(s) 499, 838
Hpy188I TCNGA 4 cut(s) 79, 540, 621, 699
Hpy188III TCNNGA 4 cut(s) 154, 445, 476, 874
Hpy8I GTNNAC 2 cut(s) 499, 838
HpyAV CCTTC 6 cut(s) 176, 208, 377, 871, 999, 1013
HpyCH4III ACNGT 2 cut(s) 115, 496
HpyCH4IV ACGT 2 cut(s) 501, 834
HpyCH4V TGCA 7 cut(s) 48, 550, 611, 713, 843, 887, 926
HpyF10VI GCNNNNNNNGC 3 cut(s) 96, 193, 763
HpyF3I CTNAG 5 cut(s) 166, 192, 402, 698, 984
HpySE526I ACGT 2 cut(s) 501, 834
Hsp92II CATG 5 cut(s) 200, 255, 891, 933, 996
HspAI GCGC 2 cut(s) 582, 820
KflI GGGWCCC 1 cut(s) 175
Kpn2I TCCGGA 1 cut(s) 444
Kzo9I GATC 8 cut(s) 156, 514, 540, 569, 669, 690, 808, 1009
LmnI GCTCC 1 cut(s) 754
Lsp1109I GCAGC 3 cut(s) 438, 544, 911
LweI GCATC 3 cut(s) 598, 622, 700
MaeI CTAG 2 cut(s) 242, 297
MaeII ACGT 2 cut(s) 501, 834
MaeIII GTNAC 2 cut(s) 812, 830
MalI GATC 8 cut(s) 158, 516, 542, 571, 671, 692, 810, 1011
MboI GATC 8 cut(s) 156, 514, 540, 569, 669, 690, 808, 1009
MboII GAAGA 4 cut(s) 299, 365, 752, 818
MflI RGATCY 3 cut(s) 514, 669, 690
MhlI GDGCHC 1 cut(s) 189
MluCI AATT 1 cut(s) 920
MlyI GAGTC 1 cut(s) 533
MroI TCCGGA 1 cut(s) 444
MseI TTAA 2 cut(s) 615, 762
MspI CCGG 4 cut(s) 63, 335, 445, 455
MspR9I CCNGG 2 cut(s) 211, 520
MvaI CCWGG 2 cut(s) 211, 520
MvnI CGCG 1 cut(s) 582
MwoI GCNNNNNNNGC 3 cut(s) 96, 193, 763
NcoI CCATGG 1 cut(s) 992
NdeII GATC 8 cut(s) 156, 514, 540, 569, 669, 690, 808, 1009
NlaIII CATG 5 cut(s) 200, 255, 891, 933, 996
NlaIV GGNNCC 6 cut(s) 29, 176, 177, 408, 516, 692
NmuCI GTSAC 1 cut(s) 830
NspI RCATGY 1 cut(s) 200
PasI CCCWGGG 1 cut(s) 519
PcsI WCGNNNNNNNCGW 2 cut(s) 903, 952
PfeI GAWTC 3 cut(s) 80, 472, 485
PfoI TCCNGGA 1 cut(s) 209
PkrI GCNGC 5 cut(s) 109, 295, 453, 559, 901
Ple19I CGATCG 1 cut(s) 543
PleI GAGTC 1 cut(s) 532
PpsI GAGTC 1 cut(s) 532
PpuMI RGGWCCY 2 cut(s) 175, 406
Psp5II RGGWCCY 2 cut(s) 175, 406
Psp6I CCWGG 2 cut(s) 209, 518
PspGI CCWGG 2 cut(s) 209, 518
PspN4I GGNNCC 6 cut(s) 29, 176, 177, 408, 516, 692
PspPI GGNCC 3 cut(s) 89, 175, 406
PspPPI RGGWCCY 2 cut(s) 175, 406
PstNI CAGNNNCTG 1 cut(s) 757
PsuI RGATCY 3 cut(s) 514, 669, 690
PvuI CGATCG 1 cut(s) 543
RsaI GTAC 3 cut(s) 437, 648, 858
RsaNI GTAC 3 cut(s) 436, 647, 857
SaqAI TTAA 2 cut(s) 615, 762
SatI GCNGC 5 cut(s) 108, 294, 452, 558, 900
Sau3AI GATC 8 cut(s) 156, 514, 540, 569, 669, 690, 808, 1009
Sau96I GGNCC 3 cut(s) 89, 175, 406
SchI GAGTC 1 cut(s) 533
ScrFI CCNGG 2 cut(s) 211, 520
SduI GDGCHC 1 cut(s) 189
SfaNI GCATC 3 cut(s) 598, 622, 700
SinI GGWCC 2 cut(s) 175, 406
SmlI CTYRAG 3 cut(s) 219, 423, 872
SmoI CTYRAG 3 cut(s) 219, 423, 872
SpeI ACTAGT 1 cut(s) 241
Sse9I AATT 1 cut(s) 920
SsiI CCGC 4 cut(s) 105, 108, 269, 293
SspI AATATT 1 cut(s) 655
SspMI CTAG 2 cut(s) 242, 297
StyD4I CCNGG 2 cut(s) 209, 518
StyI CCWWGG 1 cut(s) 992
TaaI ACNGT 2 cut(s) 115, 496
TaiI ACGT 2 cut(s) 504, 837
TaqI TCGA 5 cut(s) 153, 159, 488, 543, 572
TasI AATT 1 cut(s) 920
TatI WGTACW 1 cut(s) 856
TauI GCSGC 2 cut(s) 110, 296
TfiI GAWTC 3 cut(s) 80, 472, 485
Tru1I TTAA 2 cut(s) 615, 762
Tru9I TTAA 2 cut(s) 615, 762
TscAI CASTG 1 cut(s) 118
TseFI GTSAC 1 cut(s) 830
TseI GCWGC 3 cut(s) 451, 557, 899
Tsp45I GTSAC 1 cut(s) 830
TspDTI ATGAA 2 cut(s) 10, 963
TspGWI ACGGA 1 cut(s) 683
TspRI CASTG 1 cut(s) 118
VpaK11BI GGWCC 2 cut(s) 175, 406
XapI RAATTY 1 cut(s) 920
XceI RCATGY 1 cut(s) 200
XcmI CCANNNNNNNNNTGG 1 cut(s) 754
XmiI GTMKAC 2 cut(s) 498, 837
XspI CTAG 2 cut(s) 242, 297
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.