pycom08g01690

Essential component of the vacuolar proton pump (V- ATPase), a multimeric enzyme that catalyzes the translocation of protons across the membranes. Required for assembly and activity of the V-ATPase

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
pyrus_communis
Chr8
OG0003618
Physical Location & Seq
Reverse (-)
1252812 .. 1257997
5186 bp
Loading structure...
UTR
Exon/CDS
Intron
N/A

Sequence Viewer

Length: 2469 bp
ATGGGGACGACGGAAGCGGCGGGAGGAGGGTGCTGTCCAACGATGGATCTGCTGCGGTCGGAGCCAATGCAGTTAATCCAACTCATCATTCCGATTGAGTCGTCTCGCCACGCCATCTCCTACCTCGGCGATCTCGGCCTCTTTCAATTCAAAGACCTCAATGCGGAAAAAAGCCCGTTCCAGCGAACATACGCCACTCAGATCAAACGGTGTGGTGAAATGGCTCGGAGGTTACGGTTCTTCAAGGAACAAATGAACAAGGCCGGCTTGTCTCCCTCAACAAGGTCCATGATTAGCAATGACATCGATCTCGATAACTTGGAGGTTAAACTTGGTGAACTTGAAGCCGAGCTGTTAGAAATTAATGCGAACAACGAACAATTACAACGCACTTACAGTGAATTGTTAGAATATAAACTTGTTCTGCAGAAGGCTGGCGAGTTTTTTAATTCAGCTCAAAGCACTGCTGCAGCTCTGCACAGAGAACTTGAAGTGCAGCATAGTATGGAAAGATCCATCGACAGTCCATTATTACTCGAACAAGAAATGACAACAGATCCATCAAAGCATGTTAAACTCGGATCTGTTAGTGGTCTTGTTCCAAGAGAAAAATCAATGGCTTTTGAAAGGATTCTGTTTCGTGCAACCAGGGGTAATGTGTTTCTGAAGCAGGCTGTGGTCAATGACCGTGTCGTTGATCCTGTGTCAGGAGAGAAGGTTGAGAAAAATGTGTTTATCATCTTCTATTCTGGAGAAAGGGCAAAGAACAAAATTCTGAAAATATGTGAAGCTTTTGGAGCAAACCGTTACCCATTTACAGATGACTTGGGAAAACAATTTCAGATGATTAATGAGGTGTCTGGAAAACTTTCAGAGCTGAAGACTACCATAGATGCTGGATTGTTGCACAGGAGCAGTTTATTACAGACAATTGGTCATCAATACGAGCAGTGGAACCTTCTGGTGAAGAAGGAAAAATCCATTTACCATACGTTAAACATGCTAAGCATTGATGTGAGTAGAATGTGTCTTGTTGCAGAAGGTTGGTGTCCTGTATATGCTTCAAACCAGATTCAAAACGCACTGCAGCGGGCATCCTTAGATAGCAACTCTCAACTTGGCGCCATATTCCAGGTTTTGCATACAAAGGAACCGCCACCGACATATTTCCGCACAAACAAATTCACTTCTGCTTTTCAAGAAATTGTTGATGCATACGGGGTCGCCAAGTATCAGGAGGCTAATCCAGGCGTATACACCATTATCACATTCCCTTTCCTTTTTGCTGTAATGTTTGGAGATTGGGGACATGGTATTTGCTTGTTGCTGGCATCACTCTATTTCATAATCCGGGAGAGGAAGTTTTCTAGCCAGAAACTCGGGGACATCATAGAAATGACATTTGGTGGCCGTTATATTATTATGATGATGGCGCTGTTCTCAATTTACACAGGACTGATATATAATGAATTCTTTTCGGTCCCATTTGAGTTATTTGGGAAATCTGCCTATGGGTGCCGTGACCCGTCTTGTAGAGACGCTACTACAGTTGGTTTGACTAAGGTTCGTGACACTTATCCATTTGGTGTGGATCCCAAATGGCATGGTTCTCGATCTGAGTTACCATTTCTTAACTCATTGAAGATGAAGATGTCAATCCTACTTGGAGTAGCCCAGATGAATCTTGGAATCGTTTTGAGTTACTTTAATGCAAAATTCTTTGCGGATAAACTGAATATCTGGTACCAATTTGTTCCTCAGATGATATTTTTAAACAGCCTGTTTGGCTACCTTTCTCTCCTAATTATTGTGAAATGGTGCACTGGTTCACAAGCCGATTTGTATCATGTAATGATATATATGTTCCTGAGTCCTACTGACGACCTCGGTGAAAATCAGCTTTTTTGGGGGCAAAGATTCCTTCAGATTTTGTTACTGCTATCGGCTCTTGTTGCTGTACCATGGATGCTGTTTCCAAAGCCTTATTTCTTGAAGAAACAGCATGAGGAGAGGCACCAAGGTCAATCCTACTCACTGCTTCACAGCGGCGAGGACCCTCTTGACGAGGAGCACCACCCAAGTTTGCCTGGTCATGAGGAATTTGAATTCACCGAGGTCTTCGTACACCAACTCATACATACCATAGAATTTGTGCTTGGAGCGGTGTCTAATACAGCTTCATATCTACGTTTATGGGCACTCAGCTTAGCTCATTCGGAGTTGTCCAGTGTATTTTACGACAAGGTTCTACTTCTAGCCTGGGGGTACAACAATGTGATCATTCTGATCATTGGCATAATCGTCTTTATATGCGCGACAGTCGGAGTGCTGCTAGTGATGGAGACCTTGAGTGCATTCTTACATGCCTTGAGGCTTCACTGGGTGGAGTTCCAGAACAAGTTTTATGAGGGCTCCGGTTACAAATTCTATCCCTTCTCATTTGCATTGCTCAGCGAGGAGGACGAGTGA
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
GO:0000323 GO:0000325 GO:0000331 GO:0003674 GO:0003824 GO:0005215 GO:0005488 GO:0005515 GO:0005575 GO:0005622 GO:0005623 GO:0005737 GO:0005764 GO:0005765 GO:0005768 GO:0005773 GO:0005774 GO:0005794 GO:0005886 GO:0006139 GO:0006163 GO:0006164 GO:0006725 GO:0006753 GO:0006754 GO:0006793 GO:0006796 GO:0006807 GO:0006810 GO:0006811 GO:0006812 GO:0006873 GO:0006875 GO:0006882 GO:0006885 GO:0006897 GO:0006909 GO:0006911 GO:0007035 GO:0008150 GO:0008152 GO:0008324 GO:0009058 GO:0009117 GO:0009123 GO:0009124 GO:0009126 GO:0009127 GO:0009141 GO:0009142 GO:0009144 GO:0009145 GO:0009150 GO:0009152 GO:0009156 GO:0009161 GO:0009165 GO:0009167 GO:0009168 GO:0009199 GO:0009201 GO:0009205 GO:0009206 GO:0009259 GO:0009260 GO:0009507 GO:0009526 GO:0009536 GO:0009678 GO:0009705 GO:0009941 GO:0009987 GO:0010008 GO:0010324 GO:0012505 GO:0012506 GO:0015075 GO:0015077 GO:0015078 GO:0015318 GO:0015399 GO:0015405 GO:0015672 GO:0015985 GO:0015986 GO:0016020 GO:0016043 GO:0016192 GO:0016462 GO:0016469 GO:0016471 GO:0016787 GO:0016817 GO:0016818 GO:0016887 GO:0017111 GO:0017144 GO:0018130 GO:0019438 GO:0019637 GO:0019693 GO:0019725 GO:0019829 GO:0019899 GO:0022607 GO:0022804 GO:0022853 GO:0022857 GO:0022890 GO:0030003 GO:0030004 GO:0030139 GO:0030641 GO:0030659 GO:0030666 GO:0030670 GO:0031090 GO:0031164 GO:0031410 GO:0031967 GO:0031975 GO:0031982 GO:0032009 GO:0032119 GO:0032991 GO:0033176 GO:0034220 GO:0034622 GO:0034641 GO:0034654 GO:0036442 GO:0042592 GO:0042623 GO:0042625 GO:0042626 GO:0043181 GO:0043226 GO:0043227 GO:0043229 GO:0043231 GO:0043492 GO:0043933 GO:0044085 GO:0044237 GO:0044238 GO:0044249 GO:0044271 GO:0044281 GO:0044422 GO:0044424 GO:0044425 GO:0044433 GO:0044434 GO:0044435 GO:0044437 GO:0044440 GO:0044444 GO:0044446 GO:0044464 GO:0044769 GO:0045335 GO:0045735 GO:0045851 GO:0046034 GO:0046390 GO:0046483 GO:0046916 GO:0046961 GO:0048878 GO:0050801 GO:0051117 GO:0051179 GO:0051234 GO:0051235 GO:0051238 GO:0051452 GO:0051453 GO:0051641 GO:0051651 GO:0055065 GO:0055067 GO:0055069 GO:0055076 GO:0055080 GO:0055082 GO:0055085 GO:0055086 GO:0061024 GO:0065003 GO:0065007 GO:0065008 GO:0070070 GO:0070071 GO:0070072 GO:0071704 GO:0071840 GO:0071944 GO:0072503 GO:0072507 GO:0072521 GO:0072522 GO:0090407 GO:0090662 GO:0097708 GO:0098588 GO:0098655 GO:0098657 GO:0098660 GO:0098662 GO:0098771 GO:0098796 GO:0098805 GO:0098852 GO:0099024 GO:0099131 GO:0099132 GO:1901135 GO:1901137 GO:1901293 GO:1901360 GO:1901362 GO:1901564 GO:1901566 GO:1901576 GO:1902600
KEGG Pathways
Metabolic & Signaling
ko00190 ko01100 ko04142 ko04145 ko04721 ko04966 ko05110 ko05120 ko05152 ko05165 ko05323 map00190 map01100 map04142 map04145 map04721 map04966 map05110 map05120 map05152 map05165 map05323
Pfam Domains
Protein Families
V_ATPase_I

Protein Analysis

823

Amino Acids

93.63

Weight (kDa)

6.03

Isoelectric Point (pI)

39.59

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
V_ATPase_I PF01496 41 - 814 2.7e-302 V-type ATPase 116kDa subunit family
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
Acc65I GGTACC 1 cut(s) 1743
AccB1I GGYRCC 4 cut(s) 1121, 1515, 1743, 2013
AccBSI CCGCTC 1 cut(s) 2162
AccI GTMKAC 1 cut(s) 1254
AccII CGCG 1 cut(s) 2315
AclWI GGATC 7 cut(s) 54, 507, 551, 589, 692, 1586, 1599
AcoI YGGCCR 1 cut(s) 1408
AcsI RAATTY 8 cut(s) 771, 1181, 1469, 1715, 2099, 2105, 2147, 2423
AcuI CTGAAG 3 cut(s) 686, 899, 1907
AcyI GRCGYC 1 cut(s) 1122
AdeI CACNNNGTG 1 cut(s) 2383
AfaI GTAC 4 cut(s) 1745, 1959, 2124, 2267
AfiI CCNNNNNNNGG 3 cut(s) 163, 282, 707
AhdI GACNNNNNGTC 1 cut(s) 933
AjnI CCWGG 5 cut(s) 647, 1131, 1246, 2086, 2258
AjuI GAANNNNNNNTTGG 6 cut(s) 72, 104, 1386, 1418, 2139, 2171
AluBI AGCT 9 cut(s) 352, 455, 473, 791, 877, 1900, 2177, 2205, 2210
AluI AGCT 9 cut(s) 352, 455, 473, 791, 877, 1900, 2177, 2205, 2210
Alw21I GWGCWC 2 cut(s) 1823, 2073
Alw26I GTCTC 4 cut(s) 108, 276, 1530, 2336
Alw44I GTGCAC 1 cut(s) 1819
AlwI GGATC 7 cut(s) 54, 507, 551, 589, 692, 1586, 1599
Ama87I CYCGRG 1 cut(s) 1379
AoxI GGCC 3 cut(s) 136, 261, 1408
ApaLI GTGCAC 1 cut(s) 1819
ApeKI GCWGC 6 cut(s) 52, 467, 470, 496, 1087, 2329
ApoI RAATTY 8 cut(s) 771, 1181, 1469, 1715, 2099, 2105, 2147, 2423
ArsI GACNNNNNNTTYG 2 cut(s) 1299, 1331
AseI ATTAAT 2 cut(s) 363, 849
Asp700I GAANNNNTTC 2 cut(s) 630, 868
Asp718I GGTACC 1 cut(s) 1743
AspLEI GCGC 3 cut(s) 1124, 1435, 2315
AspS9I GGNCC 3 cut(s) 285, 1480, 2053
AsuC2I CCSGG 1 cut(s) 1352
AsuHPI GGTGA 5 cut(s) 227, 347, 976, 1901, 2101
AvaI CYCGRG 1 cut(s) 1379
AvaII GGWCC 3 cut(s) 285, 1480, 2053
BaeGI GKGCMC 2 cut(s) 1823, 2200
BamHI GGATCC 1 cut(s) 1591
BanI GGYRCC 4 cut(s) 1121, 1515, 1743, 2013
BanII GRGCYC 1 cut(s) 2414
BbsI GAAGAC 2 cut(s) 887, 2110
Bbv12I GWGCWC 2 cut(s) 1823, 2073
BbvI GCAGC 6 cut(s) 39, 454, 482, 508, 1099, 2316
BccI CCATC 6 cut(s) 37, 122, 524, 568, 1423, 2332
BceAI ACGGC 2 cut(s) 1395, 1503
BcgI CGANNNNNNTGC 6 cut(s) 31, 65, 286, 320, 1592, 1626
BciT130I CCWGG 5 cut(s) 649, 1133, 1248, 2088, 2260
BclI TGATCA 2 cut(s) 2277, 2286
BcnI CCSGG 1 cut(s) 1352
BcoDI GTCTC 4 cut(s) 108, 276, 1530, 2336
BfaI CTAG 3 cut(s) 1368, 2255, 2333
BfmI CTRYAG 4 cut(s) 425, 468, 1085, 1545
BfoI RGCGCY 2 cut(s) 1125, 1436
BglI GCCNNNNNGGC 1 cut(s) 1785
BisI GCNGC 8 cut(s) 18, 53, 468, 471, 497, 1088, 2047, 2330
BlpI GCTNAGC 3 cut(s) 1004, 2206, 2450
BlsI GCNGC 8 cut(s) 19, 54, 469, 472, 498, 1089, 2048, 2331
Bme1390I CCNGG 6 cut(s) 649, 1133, 1248, 1352, 2088, 2260
Bme18I GGWCC 3 cut(s) 285, 1480, 2053
BmeRI GACNNNNNGTC 1 cut(s) 933
BmeT110I CYCGRG 1 cut(s) 1379
BmgT120I GGNCC 3 cut(s) 285, 1480, 2053
BmrFI CCNGG 6 cut(s) 649, 1133, 1248, 1352, 2088, 2260
BmrI ACTGGG 1 cut(s) 2389
BmsI GCATC 5 cut(s) 883, 1103, 1201, 1340, 1956
BmuI ACTGGG 1 cut(s) 2389
BpiI GAAGAC 2 cut(s) 887, 2110
BpmI CTGGAG 1 cut(s) 771
Bpu1102I GCTNAGC 3 cut(s) 1004, 2206, 2450
BpuEI CTTGAG 2 cut(s) 2368, 2389
BpuMI CCSGG 1 cut(s) 1352
Bsa29I ATCGAT 1 cut(s) 306
BsaBI GATNNNNATC 2 cut(s) 1655, 1842
BsaHI GRCGYC 1 cut(s) 1122
BsaI GGTCTC 1 cut(s) 2336
BsaJI CCNNGG 7 cut(s) 124, 648, 1885, 1961, 2017, 2112, 2259
BsaWI WCCGGW 1 cut(s) 2414
BsaXI ACNNNNNCTCC 7 cut(s) 101, 131, 702, 732, 1346, 1376, 2450
Bsc4I CCNNNNNNNGG 3 cut(s) 163, 282, 707
Bse118I RCCGGY 1 cut(s) 263
Bse1I ACTGG 3 cut(s) 1828, 2226, 2384
Bse3DI GCAATG 2 cut(s) 304, 2444
Bse8I GATNNNNATC 2 cut(s) 1655, 1842
BseBI CCWGG 5 cut(s) 649, 1133, 1248, 2088, 2260
BseCI ATCGAT 1 cut(s) 306
BseDI CCNNGG 7 cut(s) 124, 648, 1885, 1961, 2017, 2112, 2259
BseGI GGATG 2 cut(s) 1094, 1971
BseJI GATNNNNATC 2 cut(s) 1655, 1842
BseLI CCNNNNNNNGG 3 cut(s) 163, 282, 707
BseMI GCAATG 2 cut(s) 304, 2444
BseMII CTCAG 6 cut(s) 212, 1608, 1772, 1859, 2215, 2464
BseNI ACTGG 3 cut(s) 1828, 2226, 2384
BseRI GAGGAG 3 cut(s) 39, 2021, 2081
BseSI GKGCMC 2 cut(s) 1823, 2200
BseXI GCAGC 6 cut(s) 39, 454, 482, 508, 1099, 2316
BsgI GTGCAG 2 cut(s) 461, 515
Bsh1236I CGCG 1 cut(s) 2315
Bsh1285I CGRYCG 1 cut(s) 59
BshFI GGCC 3 cut(s) 138, 263, 1410
BshNI GGYRCC 4 cut(s) 1121, 1515, 1743, 2013
BshVI ATCGAT 1 cut(s) 306
BsiEI CGRYCG 1 cut(s) 59
BsiHKAI GWGCWC 2 cut(s) 1823, 2073
BsiHKCI CYCGRG 1 cut(s) 1379
BsiSI CCGG 3 cut(s) 264, 1351, 2415
BslFI GGGAC 4 cut(s) 19, 1320, 1397, 1466
BslI CCNNNNNNNGG 3 cut(s) 163, 282, 707
BsmAI GTCTC 4 cut(s) 108, 276, 1530, 2336
BsmBI CGTCTC 2 cut(s) 108, 1530
BsmFI GGGAC 4 cut(s) 19, 1320, 1397, 1466
BsmI GAATGC 1 cut(s) 2354
BsnI GGCC 3 cut(s) 138, 263, 1410
Bso31I GGTCTC 1 cut(s) 2336
BsoBI CYCGRG 1 cut(s) 1379
Bsp1286I GDGCHC 4 cut(s) 1823, 2073, 2200, 2414
Bsp1720I GCTNAGC 3 cut(s) 1004, 2206, 2450
Bsp19I CCATGG 1 cut(s) 1961
BspANI GGCC 3 cut(s) 138, 263, 1410
BspCNI CTCAG 6 cut(s) 211, 1609, 1771, 1860, 2214, 2463
BspDI ATCGAT 1 cut(s) 306
BspFNI CGCG 1 cut(s) 2315
BspHI TCATGA 1 cut(s) 2092
BspMAI CTGCAG 3 cut(s) 429, 472, 1089
BspPI GGATC 7 cut(s) 54, 507, 551, 589, 692, 1586, 1599
BspT107I GGYRCC 4 cut(s) 1121, 1515, 1743, 2013
BspTNI GGTCTC 1 cut(s) 2336
BsrBI CCGCTC 1 cut(s) 2162
BsrDI GCAATG 2 cut(s) 304, 2444
BsrFI RCCGGY 1 cut(s) 263
BsrI ACTGG 3 cut(s) 1828, 2226, 2384
BssAI RCCGGY 1 cut(s) 263
BssECI CCNNGG 7 cut(s) 124, 648, 1885, 1961, 2017, 2112, 2259
BssNAI GTATAC 1 cut(s) 1255
BssNI GRCGYC 1 cut(s) 1122
BssT1I CCWWGG 2 cut(s) 1961, 2017
Bst1107I GTATAC 1 cut(s) 1255
Bst2UI CCWGG 5 cut(s) 649, 1133, 1248, 2088, 2260
Bst4CI ACNGT 8 cut(s) 210, 237, 398, 524, 689, 806, 1549, 2320
BstACI GRCGYC 1 cut(s) 1122
BstC8I GCNNGC 5 cut(s) 265, 436, 672, 1092, 1329
BstDSI CCRYGG 1 cut(s) 1961
BstENI CCTNNNNNAGG 2 cut(s) 280, 705
BstF5I GGATG 2 cut(s) 1094, 1971
BstFNI CGCG 1 cut(s) 2315
BstH2I RGCGCY 2 cut(s) 1125, 1436
BstHHI GCGC 3 cut(s) 1124, 1435, 2315
BstMAI GTCTC 4 cut(s) 108, 276, 1530, 2336
BstMCI CGRYCG 1 cut(s) 59
BstMWI GCNNNNNNNGC 5 cut(s) 61, 135, 797, 1785, 1952
BstNI CCWGG 5 cut(s) 649, 1133, 1248, 2088, 2260
BstNSI RCATGY 3 cut(s) 572, 1003, 2366
BstSCI CCNGG 6 cut(s) 647, 1131, 1246, 1350, 2086, 2258
BstSFI CTRYAG 4 cut(s) 425, 468, 1085, 1545
BstSLI GKGCMC 2 cut(s) 1823, 2200
BstUI CGCG 1 cut(s) 2315
BstV1I GCAGC 6 cut(s) 39, 454, 482, 508, 1099, 2316
BstV2I GAAGAC 2 cut(s) 887, 2110
BstX2I RGATCY 5 cut(s) 46, 512, 556, 581, 1591
BstYI RGATCY 5 cut(s) 46, 512, 556, 581, 1591
BstZ17I GTATAC 1 cut(s) 1255
Bsu15I ATCGAT 1 cut(s) 306
BsuRI GGCC 3 cut(s) 138, 263, 1410
BsuTUI ATCGAT 1 cut(s) 306
BtgI CCRYGG 1 cut(s) 1961
BtsCI GGATG 2 cut(s) 1094, 1971
BtsI GCAGTG 4 cut(s) 462, 956, 1082, 2033
BtsIMutI CAGTG 8 cut(s) 403, 462, 956, 1082, 1821, 2033, 2233, 2377
Cac8I GCNNGC 5 cut(s) 265, 436, 672, 1092, 1329
CciI TCATGA 1 cut(s) 2092
CfoI GCGC 3 cut(s) 1124, 1435, 2315
Cfr10I RCCGGY 1 cut(s) 263
Cfr13I GGNCC 3 cut(s) 285, 1480, 2053
ClaI ATCGAT 1 cut(s) 306
CseI GACGC 1 cut(s) 1547
Csp6I GTAC 4 cut(s) 1744, 1958, 2123, 2266
CspCI CAANNNNNGTGG 2 cut(s) 193, 228
CviQI GTAC 4 cut(s) 1744, 1958, 2123, 2266
DinI GGCGCC 1 cut(s) 1123
DraI TTTAAA 1 cut(s) 1773
DraIII CACNNNGTG 1 cut(s) 2383
DriI GACNNNNNGTC 1 cut(s) 933
EaeI YGGCCR 1 cut(s) 1408
Eam1105I GACNNNNNGTC 1 cut(s) 933
Eco130I CCWWGG 2 cut(s) 1961, 2017
Eco24I GRGCYC 1 cut(s) 2414
Eco31I GGTCTC 1 cut(s) 2336
Eco47I GGWCC 3 cut(s) 285, 1480, 2053
Eco57I CTGAAG 3 cut(s) 686, 899, 1907
Eco88I CYCGRG 1 cut(s) 1379
EcoNI CCTNNNNNAGG 2 cut(s) 280, 705
EcoO109I RGGNCCY 1 cut(s) 2053
EcoRI GAATTC 2 cut(s) 1469, 2105
EcoRII CCWGG 5 cut(s) 647, 1131, 1246, 2086, 2258
EcoT14I CCWWGG 2 cut(s) 1961, 2017
EcoT22I ATGCAT 1 cut(s) 1216
EcoT38I GRGCYC 1 cut(s) 2414
EgeI GGCGCC 1 cut(s) 1123
EheI GGCGCC 1 cut(s) 1123
ErhI CCWWGG 2 cut(s) 1961, 2017
Esp3I CGTCTC 2 cut(s) 108, 1530
FalI AAGNNNNNCTT 2 cut(s) 251, 283
FaqI GGGAC 4 cut(s) 19, 1320, 1397, 1466
FauI CCCGC 2 cut(s) 13, 1083
FbaI TGATCA 2 cut(s) 2277, 2286
FblI GTMKAC 1 cut(s) 1254
Fnu4HI GCNGC 8 cut(s) 18, 53, 468, 471, 497, 1088, 2047, 2330
FokI GGATG 2 cut(s) 1081, 1978
FriOI GRGCYC 1 cut(s) 2414
Fsp4HI GCNGC 8 cut(s) 18, 53, 468, 471, 497, 1088, 2047, 2330
FspBI CTAG 3 cut(s) 1368, 2255, 2333
GlaI GCGC 3 cut(s) 1123, 1434, 2314
GluI GCNGC 8 cut(s) 18, 53, 468, 471, 497, 1088, 2047, 2330
GsuI CTGGAG 1 cut(s) 771
HaeII RGCGCY 2 cut(s) 1125, 1436
HaeIII GGCC 3 cut(s) 138, 263, 1410
HapII CCGG 3 cut(s) 264, 1351, 2415
HgaI GACGC 1 cut(s) 1547
HhaI GCGC 3 cut(s) 1124, 1435, 2315
Hin1I GRCGYC 1 cut(s) 1122
Hin6I GCGC 3 cut(s) 1122, 1433, 2313
HinP1I GCGC 3 cut(s) 1122, 1433, 2313
HindIII AAGCTT 1 cut(s) 789
HinfI GANTC 7 cut(s) 98, 631, 1072, 1681, 1689, 1870, 1917
HpaII CCGG 3 cut(s) 264, 1351, 2415
HphI GGTGA 5 cut(s) 227, 347, 976, 1901, 2101
Hpy166II GTNNAC 5 cut(s) 338, 1255, 1821, 1829, 2125
Hpy8I GTNNAC 5 cut(s) 338, 1255, 1821, 1829, 2125
Hpy99I CGWCG 1 cut(s) 13
HpyAV CCTTC 7 cut(s) 424, 709, 964, 968, 1034, 1931, 2443
HpyCH4III ACNGT 8 cut(s) 210, 237, 398, 524, 689, 806, 1549, 2320
HpyCH4IV ACGT 2 cut(s) 992, 2188
HpyF10VI GCNNNNNNNGC 5 cut(s) 61, 135, 797, 1785, 1952
HpySE526I ACGT 2 cut(s) 992, 2188
Hsp92I GRCGYC 1 cut(s) 1122
HspAI GCGC 3 cut(s) 1122, 1433, 2313
KasI GGCGCC 1 cut(s) 1121
KpnI GGTACC 1 cut(s) 1747
KroI GCCGGC 1 cut(s) 263
KroNI GCCGGC 1 cut(s) 265
Ksp22I TGATCA 2 cut(s) 2277, 2286
LmnI GCTCC 6 cut(s) 61, 797, 912, 2068, 2159, 2417
Lsp1109I GCAGC 6 cut(s) 39, 454, 482, 508, 1099, 2316
LweI GCATC 5 cut(s) 883, 1103, 1201, 1340, 1956
MaeI CTAG 3 cut(s) 1368, 2255, 2333
MaeII ACGT 2 cut(s) 992, 2188
MaeIII GTNAC 8 cut(s) 231, 806, 1520, 1568, 1620, 1700, 1932, 2417
MbiI CCGCTC 1 cut(s) 2162
MboII GAAGA 8 cut(s) 232, 733, 892, 979, 1654, 1660, 2005, 2110
MfeI CAATTG 1 cut(s) 930
MflI RGATCY 5 cut(s) 46, 512, 556, 581, 1591
MhlI GDGCHC 4 cut(s) 1823, 2073, 2200, 2414
Mly113I GGCGCC 1 cut(s) 1122
MlyI GAGTC 2 cut(s) 107, 1879
MmeI TCCRAC 4 cut(s) 39, 62, 103, 2302
Mph1103I ATGCAT 1 cut(s) 1216
MroNI GCCGGC 1 cut(s) 263
MroXI GAANNNNTTC 2 cut(s) 630, 868
MslI CAYNNNNRTG 2 cut(s) 1013, 1394
MspA1I CMGCKG 2 cut(s) 1090, 2046
MspI CCGG 3 cut(s) 264, 1351, 2415
MspR9I CCNGG 6 cut(s) 649, 1133, 1248, 1352, 2088, 2260
MunI CAATTG 1 cut(s) 930
Mva1269I GAATGC 1 cut(s) 2354
MvaI CCWGG 5 cut(s) 649, 1133, 1248, 2088, 2260
MvnI CGCG 1 cut(s) 2315
MwoI GCNNNNNNNGC 5 cut(s) 61, 135, 797, 1785, 1952
NaeI GCCGGC 1 cut(s) 265
NarI GGCGCC 1 cut(s) 1122
NciI CCSGG 1 cut(s) 1352
NcoI CCATGG 1 cut(s) 1961
NgoMIV GCCGGC 1 cut(s) 263
NmeAIII GCCGAG 3 cut(s) 105, 114, 373
NmuCI GTSAC 2 cut(s) 1520, 1568
NsiI ATGCAT 1 cut(s) 1216
NspI RCATGY 3 cut(s) 572, 1003, 2366
PagI TCATGA 1 cut(s) 2092
PctI GAATGC 1 cut(s) 2354
PdiI GCCGGC 1 cut(s) 265
PdmI GAANNNNTTC 2 cut(s) 630, 868
PfeI GAWTC 5 cut(s) 631, 1072, 1681, 1689, 1917
PflFI GACNNNGTC 1 cut(s) 689
PfoI TCCNGGA 1 cut(s) 1350
PkrI GCNGC 8 cut(s) 19, 54, 469, 472, 498, 1089, 2048, 2331
PleI GAGTC 2 cut(s) 106, 1878
PluTI GGCGCC 1 cut(s) 1125
PpsI GAGTC 2 cut(s) 106, 1878
PpuMI RGGWCCY 1 cut(s) 2053
PshBI ATTAAT 2 cut(s) 363, 849
Psp5II RGGWCCY 1 cut(s) 2053
Psp6I CCWGG 5 cut(s) 647, 1131, 1246, 2086, 2258
PspGI CCWGG 5 cut(s) 647, 1131, 1246, 2086, 2258
PspPI GGNCC 3 cut(s) 285, 1480, 2053
PspPPI RGGWCCY 1 cut(s) 2053
PstI CTGCAG 3 cut(s) 429, 472, 1089
PsuI RGATCY 5 cut(s) 46, 512, 556, 581, 1591
PsyI GACNNNGTC 1 cut(s) 689
RsaI GTAC 4 cut(s) 1745, 1959, 2124, 2267
RsaNI GTAC 4 cut(s) 1744, 1958, 2123, 2266
RseI CAYNNNNRTG 2 cut(s) 1013, 1394
SatI GCNGC 8 cut(s) 18, 53, 468, 471, 497, 1088, 2047, 2330
Sau96I GGNCC 3 cut(s) 285, 1480, 2053
SchI GAGTC 2 cut(s) 107, 1879
ScrFI CCNGG 6 cut(s) 649, 1133, 1248, 1352, 2088, 2260
SduI GDGCHC 4 cut(s) 1823, 2073, 2200, 2414
SfaNI GCATC 5 cut(s) 883, 1103, 1201, 1340, 1956
SfcI CTRYAG 4 cut(s) 425, 468, 1085, 1545
SfoI GGCGCC 1 cut(s) 1123
SinI GGWCC 3 cut(s) 285, 1480, 2053
SmiMI CAYNNNNRTG 2 cut(s) 1013, 1394
SmlI CTYRAG 2 cut(s) 2347, 2368
SmoI CTYRAG 2 cut(s) 2347, 2368
SspDI GGCGCC 1 cut(s) 1121
SspMI CTAG 3 cut(s) 1368, 2255, 2333
StyD4I CCNGG 6 cut(s) 647, 1131, 1246, 1350, 2086, 2258
StyI CCWWGG 2 cut(s) 1961, 2017
TaaI ACNGT 8 cut(s) 210, 237, 398, 524, 689, 806, 1549, 2320
TaiI ACGT 2 cut(s) 995, 2191
TaqI TCGA 5 cut(s) 306, 312, 519, 537, 1612
TaqII GACCGA 1 cut(s) 1468
TauI GCSGC 2 cut(s) 20, 2049
TfiI GAWTC 5 cut(s) 631, 1072, 1681, 1689, 1917
TscAI CASTG 8 cut(s) 403, 469, 956, 1089, 1828, 2040, 2233, 2384
TseFI GTSAC 2 cut(s) 1520, 1568
TseI GCWGC 6 cut(s) 52, 467, 470, 496, 1087, 2329
Tsp45I GTSAC 2 cut(s) 1520, 1568
TspDTI ATGAA 6 cut(s) 269, 1333, 1482, 1661, 1694, 2169
TspGWI ACGGA 1 cut(s) 26
TspRI CASTG 8 cut(s) 403, 469, 956, 1089, 1828, 2040, 2233, 2384
Tth111I GACNNNGTC 1 cut(s) 689
VneI GTGCAC 1 cut(s) 1819
VpaK11BI GGWCC 3 cut(s) 285, 1480, 2053
VspI ATTAAT 2 cut(s) 363, 849
XagI CCTNNNNNAGG 2 cut(s) 280, 705
XapI RAATTY 8 cut(s) 771, 1181, 1469, 1715, 2099, 2105, 2147, 2423
XceI RCATGY 3 cut(s) 572, 1003, 2366
XcmI CCANNNNNNNNNTGG 1 cut(s) 1682
XmiI GTMKAC 1 cut(s) 1254
XmnI GAANNNNTTC 2 cut(s) 630, 868
XspI CTAG 3 cut(s) 1368, 2255, 2333
Zsp2I ATGCAT 1 cut(s) 1216
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.