Basic Information

Type: gene

Biological Identity

Species

pyrus_communis

Chromosome

Chr8

Orthogroup ID

OG0007703

Physical Location & Seq

Genomic Coordinates Forward (+)

5717988 .. 5718806

Gene Structure 819 bp

Loading structure...

UTR

Exon/CDS

Intron

Transcript / Protein ID

pycom08g07100.1

Sequence Viewer

CDS Protein Promoter (2k)

Length: 819 bp

ATGGCTCTGCAAGCTCTCAACTCGCCAACACTCGCCGGATCGTCTCCTTTCCAGCTCGAGACCAAAGCCTCGAGCTTCAGCTATGTCGAGCCGTGGACCAAACGCAAGCGTTCCAAGCGTCCACGCCTTGACACCTCTCATACCGAGGAGGAGGAGTACCTCGCCCTCTGCCTCATCATGCTCGCCCGCGGTAATAGAGATGGAGACCTCGCCGATACCATGGCCACTAAAGCAACAACCTCCGCCGCCTCACACCAGATCATCACTCAATCCCCTTCTATGGAGCCCTCCACGTCAGCAGCGCCGCCGGCTAACCTGAGCTACAAGTGCTCTGTTTGCGACAAGAGCTTCTCTTCTTACCAGGCCCTCGGAGGACACAAGGCCAGCCACCGAAAAGGCTCTGCTGCCGGATCGGCCGTCGAAGGCCCGTCCACGTCCTCAACCACCACAACCTCCGCCACCACCACCGCCACCCCTTCGGGAAGGTCCCACGAGTGCTCCATATGCCACAAGTCTTTCCCGACCGGTCAAGCCTTGGGCGGCCACAAGCGCTGCCACTACGATGGCGGCGCCGCAGGATCCACCGCTACCACAAGCGCTATAACTTCCTCTGAGGGAGTCGGGTCAACTTCTCACGCGGTCAGCCACGGCCACCCCCGAGAGACGTTTGACCTGAACCTGCCGGCGCTGCCCGACTTCTCCCGCGACTTCTTCGTATCCGGCGAGGACGAGGTGGAGAGCCCACACCCGACGAAGAAGCCCAGGCTCCTGCTGATGGTGAAGCCCAAAATGGAGATTTCACAGGACCAACAAAATTAG

Functional Annotation

Gene Ontology

Molecular Function
Biological Process
Cellular Component

GO:0000003 GO:0001067 GO:0001101 GO:0003006 GO:0003674 GO:0003676 GO:0003677 GO:0003700 GO:0005488 GO:0005575 GO:0005622 GO:0005623 GO:0005634 GO:0006355 GO:0006950 GO:0006970 GO:0006972 GO:0006979 GO:0007275 GO:0008150 GO:0008152 GO:0009266 GO:0009314 GO:0009409 GO:0009414 GO:0009415 GO:0009416 GO:0009611 GO:0009628 GO:0009642 GO:0009644 GO:0009651 GO:0009719 GO:0009725 GO:0009737 GO:0009790 GO:0009791 GO:0009793 GO:0009889 GO:0009890 GO:0009892 GO:0009987 GO:0010033 GO:0010035 GO:0010117 GO:0010154 GO:0010200 GO:0010243 GO:0010468 GO:0010556 GO:0010558 GO:0010605 GO:0010629 GO:0015979 GO:0019219 GO:0019222 GO:0022414 GO:0031323 GO:0031324 GO:0031326 GO:0031327 GO:0032501 GO:0032502 GO:0033993 GO:0035264 GO:0040007 GO:0042221 GO:0042493 GO:0042538 GO:0043226 GO:0043227 GO:0043229 GO:0043231 GO:0043565 GO:0044212 GO:0044237 GO:0044424 GO:0044464 GO:0045892 GO:0045934 GO:0048316 GO:0048519 GO:0048523 GO:0048589 GO:0048608 GO:0048731 GO:0048856 GO:0050789 GO:0050794 GO:0050896 GO:0051171 GO:0051172 GO:0051252 GO:0051253 GO:0060255 GO:0061458 GO:0065007 GO:0080090 GO:0097159 GO:0097305 GO:0140110 GO:1901363 GO:1901698 GO:1901700 GO:1902679 GO:1903506 GO:1903507 GO:2000112 GO:2000113 GO:2001141

KEGG Pathways

Metabolic & Signaling

No pathways identified.

Pfam Domains

Protein Families

zf-C2H2_6

Protein Analysis

273

Amino Acids

28.82

Weight (kDa)

7.71

Isoelectric Point (pI)

58.29

Instability Index

Protein Domains (Pfam)

Domain Name	Pfam ID	Position	E-value	Description
zf-C2H2_6	PF13912	108 - 132	1.3e-13	C2H2-type zinc finger
zf-C2H2_6	PF13912	164 - 188	4.2e-12	C2H2-type zinc finger

Hydrophobicity Profile (Kyte-Doolittle)

AI Structure Prediction Report

Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter

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Align Labels (Cladogram)

Image

Tree File

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Publication-ready

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Orthologous Genes (Group: OG0007703)

Species	Orthologous Gene IDs
arabidopsis_thaliana	AT1G27730 AT3G49930 AT5G04340 AT5G67450
fragaria_vesca	FvH4_2g33250
malus_domestica	MD01G1041900.v1.1 MD03G1122300.v1.1 MD08G1086500.v1.1 MD15G1071400.v1.1
prunus_persica	Prupe.1G424300_v2.0.a1
pyrus_communis	pycom08g07100 pycom15g06720
rosa_chinensis	RchiOBHm_Chr6g0309391
rosa_laevigata	RLG00000010570
rosa_multiflora	Rmu_co8321009.1_g000001 Rmu_sc0012466.1_g000004 Rmu_sc0017306.1_g000001
rosa_roxburghii	Rroxscaffold_7G00159440
rosa_rugosa	Rorug06G0371800
rosa_samantha	Rh6AG484900 Rh6BG494100 Rh6CG499000 Rh6DG485300
rosa_wichuraiana	Rw6G042230

Restriction Enzyme Sites

1 / 10

Enzyme	Recognition Site	Cut Count	Positions (bp)
Acc36I	ACCTGC	1 cut(s)	687
AccB1I	GGYRCC	1 cut(s)	569
AccII	CGCG	3 cut(s)	189, 638, 705
AclWI	GGATC	4 cut(s)	46, 418, 573, 586
AcoI	YGGCCR	4 cut(s)	222, 414, 541, 649
AcuI	CTGAAG	1 cut(s)	61
AcyI	GRCGYC	1 cut(s)	570
AfaI	GTAC	1 cut(s)	158
AfeI	AGCGCT	2 cut(s)	551, 598
AfiI	CCNNNNNNNGG	2 cut(s)	280, 775
AgeI	ACCGGT	1 cut(s)	524
AjiI	CACGTC	2 cut(s)	294, 435
AjnI	CCWGG	2 cut(s)	360, 761
AluBI	AGCT	6 cut(s)	14, 55, 75, 81, 321, 348
AluI	AGCT	6 cut(s)	14, 55, 75, 81, 321, 348
Alw21I	GWGCWC	2 cut(s)	332, 500
Alw26I	GTCTC	4 cut(s)	48, 53, 198, 656
AlwI	GGATC	4 cut(s)	46, 418, 573, 586
Ama87I	CYCGRG	3 cut(s)	56, 70, 657
Aor51HI	AGCGCT	2 cut(s)	551, 598
AoxI	GGCC	7 cut(s)	222, 363, 381, 414, 424, 541, 649
ApeKI	GCWGC	4 cut(s)	299, 404, 552, 688
AsiGI	ACCGGT	1 cut(s)	524
AspLEI	GCGC	5 cut(s)	304, 552, 572, 599, 688
AspS9I	GGNCC	5 cut(s)	96, 364, 425, 486, 805
AsuHPI	GGTGA	1 cut(s)	790
AvaI	CYCGRG	3 cut(s)	56, 70, 657
AvaII	GGWCC	3 cut(s)	96, 486, 805
BalI	TGGCCA	1 cut(s)	224
BamHI	GGATCC	1 cut(s)	578
BanI	GGYRCC	1 cut(s)	569
BanII	GRGCYC	2 cut(s)	288, 743
BauI	CACGAG	1 cut(s)	491
Bbv12I	GWGCWC	2 cut(s)	332, 500
BbvI	GCAGC	4 cut(s)	311, 391, 539, 675
BccI	CCATC	3 cut(s)	194, 557, 769
BceAI	ACGGC	3 cut(s)	76, 401, 664
BciT130I	CCWGG	2 cut(s)	362, 763
BciVI	GTATCC	1 cut(s)	727
BcoDI	GTCTC	4 cut(s)	48, 53, 198, 656
BfoI	RGCGCY	5 cut(s)	305, 553, 573, 600, 689
BfuAI	ACCTGC	1 cut(s)	687
BfuI	GTATCC	1 cut(s)	727
BglI	GCCNNNNNGGC	1 cut(s)	413
BisI	GCNGC	9 cut(s)	246, 300, 305, 405, 541, 553, 568, 573, 689
BlsI	GCNGC	9 cut(s)	247, 301, 306, 406, 542, 554, 569, 574, 690
Bme1390I	CCNGG	2 cut(s)	362, 763
Bme18I	GGWCC	3 cut(s)	96, 486, 805
BmeT110I	CYCGRG	3 cut(s)	56, 70, 657
BmgBI	CACGTC	2 cut(s)	294, 435
BmgT120I	GGNCC	5 cut(s)	96, 364, 425, 486, 805
BmiI	GGNNCC	5 cut(s)	285, 488, 571, 580, 767
BmrFI	CCNGG	2 cut(s)	362, 763
Bpu10I	CCTNAGC	1 cut(s)	317
BsaHI	GRCGYC	1 cut(s)	570
BsaI	GGTCTC	2 cut(s)	53, 198
BsaJI	CCNNGG	8 cut(s)	92, 144, 187, 219, 367, 534, 646, 761
BsaWI	WCCGGW	1 cut(s)	524
BsaXI	ACNNNNNCTCC	4 cut(s)	140, 170, 482, 512
Bsc4I	CCNNNNNNNGG	2 cut(s)	280, 775
Bse118I	RCCGGY	3 cut(s)	307, 524, 682
BseBI	CCWGG	2 cut(s)	362, 763
BseDI	CCNNGG	8 cut(s)	92, 144, 187, 219, 367, 534, 646, 761
BseLI	CCNNNNNNNGG	2 cut(s)	280, 775
BseMII	CTCAG	2 cut(s)	308, 603
BseRI	GAGGAG	3 cut(s)	161, 164, 167
BseX3I	CGGCCG	1 cut(s)	414
BseXI	GCAGC	4 cut(s)	311, 391, 539, 675
Bsh1236I	CGCG	3 cut(s)	189, 638, 705
Bsh1285I	CGRYCG	2 cut(s)	417, 525
BshFI	GGCC	7 cut(s)	224, 365, 383, 416, 426, 543, 651
BshNI	GGYRCC	1 cut(s)	569
BshTI	ACCGGT	1 cut(s)	524
BsiEI	CGRYCG	2 cut(s)	417, 525
BsiHKAI	GWGCWC	2 cut(s)	332, 500
BsiHKCI	CYCGRG	3 cut(s)	56, 70, 657
BsiSI	CCGG	6 cut(s)	36, 308, 408, 525, 683, 720
BslFI	GGGAC	1 cut(s)	472
BslI	CCNNNNNNNGG	2 cut(s)	280, 775
BsmAI	GTCTC	4 cut(s)	48, 53, 198, 656
BsmBI	CGTCTC	2 cut(s)	48, 656
BsmFI	GGGAC	1 cut(s)	472
BsnI	GGCC	7 cut(s)	224, 365, 383, 416, 426, 543, 651
Bso31I	GGTCTC	2 cut(s)	53, 198
BsoBI	CYCGRG	3 cut(s)	56, 70, 657
Bsp1286I	GDGCHC	4 cut(s)	288, 332, 500, 743
Bsp143I	GATC	4 cut(s)	38, 258, 410, 578
Bsp19I	CCATGG	1 cut(s)	219
BspANI	GGCC	7 cut(s)	224, 365, 383, 416, 426, 543, 651
BspCNI	CTCAG	2 cut(s)	309, 604
BspFNI	CGCG	3 cut(s)	189, 638, 705
BspLI	GGNNCC	5 cut(s)	285, 488, 571, 580, 767
BspMI	ACCTGC	1 cut(s)	687
BspPI	GGATC	4 cut(s)	46, 418, 573, 586
BspT107I	GGYRCC	1 cut(s)	569
BspTNI	GGTCTC	2 cut(s)	53, 198
BsrFI	RCCGGY	3 cut(s)	307, 524, 682
BssAI	RCCGGY	3 cut(s)	307, 524, 682
BssECI	CCNNGG	8 cut(s)	92, 144, 187, 219, 367, 534, 646, 761
BssMI	GATC	4 cut(s)	38, 258, 410, 578
BssNI	GRCGYC	1 cut(s)	570
BssSI	CACGAG	1 cut(s)	491
BssT1I	CCWWGG	2 cut(s)	219, 534
Bst2BI	CACGAG	1 cut(s)	491
Bst2UI	CCWGG	2 cut(s)	362, 763
Bst6I	CTCTTC	1 cut(s)	358
BstACI	GRCGYC	1 cut(s)	570
BstC8I	GCNNGC	7 cut(s)	12, 107, 183, 187, 309, 385, 684
BstDEI	CTNAG	2 cut(s)	317, 612
BstDSI	CCRYGG	4 cut(s)	92, 187, 219, 646
BstFNI	CGCG	3 cut(s)	189, 638, 705
BstH2I	RGCGCY	5 cut(s)	305, 553, 573, 600, 689
BstHHI	GCGC	5 cut(s)	304, 552, 572, 599, 688
BstKTI	GATC	4 cut(s)	41, 261, 413, 581
BstMAI	GTCTC	4 cut(s)	48, 53, 198, 656
BstMBI	GATC	4 cut(s)	38, 258, 410, 578
BstMCI	CGRYCG	2 cut(s)	417, 525
BstNI	CCWGG	2 cut(s)	362, 763
BstSCI	CCNGG	2 cut(s)	360, 761
BstUI	CGCG	3 cut(s)	189, 638, 705
BstV1I	GCAGC	4 cut(s)	311, 391, 539, 675
BstX2I	RGATCY	1 cut(s)	578
BstXI	CCANNNNNNTGG	1 cut(s)	563
BstYI	RGATCY	1 cut(s)	578
BstZI	CGGCCG	1 cut(s)	414
BsuI	GTATCC	1 cut(s)	727
BsuRI	GGCC	7 cut(s)	224, 365, 383, 416, 426, 543, 651
BtgI	CCRYGG	4 cut(s)	92, 187, 219, 646
BtrI	CACGTC	2 cut(s)	294, 435
BveI	ACCTGC	1 cut(s)	687
Cac8I	GCNNGC	7 cut(s)	12, 107, 183, 187, 309, 385, 684
CfoI	GCGC	5 cut(s)	304, 552, 572, 599, 688
Cfr10I	RCCGGY	3 cut(s)	307, 524, 682
Cfr13I	GGNCC	5 cut(s)	96, 364, 425, 486, 805
Cfr42I	CCGCGG	1 cut(s)	190
CseI	GACGC	1 cut(s)	107
Csp6I	GTAC	1 cut(s)	157
CspAI	ACCGGT	1 cut(s)	524
CviAII	CATG	2 cut(s)	178, 220
CviQI	GTAC	1 cut(s)	157
DdeI	CTNAG	2 cut(s)	317, 612
DinI	GGCGCC	1 cut(s)	571
DpnI	GATC	4 cut(s)	40, 260, 412, 580
DpnII	GATC	4 cut(s)	38, 258, 410, 578
EaeI	YGGCCR	4 cut(s)	222, 414, 541, 649
EagI	CGGCCG	1 cut(s)	414
Eam1104I	CTCTTC	1 cut(s)	358
EarI	CTCTTC	1 cut(s)	358
EciI	GGCGGA	2 cut(s)	232, 445
EclXI	CGGCCG	1 cut(s)	414
Eco130I	CCWWGG	2 cut(s)	219, 534
Eco24I	GRGCYC	2 cut(s)	288, 743
Eco31I	GGTCTC	2 cut(s)	53, 198
Eco47I	GGWCC	3 cut(s)	96, 486, 805
Eco47III	AGCGCT	2 cut(s)	551, 598
Eco52I	CGGCCG	1 cut(s)	414
Eco57I	CTGAAG	1 cut(s)	61
Eco88I	CYCGRG	3 cut(s)	56, 70, 657
EcoO109I	RGGNCCY	2 cut(s)	364, 486
EcoRII	CCWGG	2 cut(s)	360, 761
EcoT14I	CCWWGG	2 cut(s)	219, 534
EcoT38I	GRGCYC	2 cut(s)	288, 743
EgeI	GGCGCC	1 cut(s)	571
EheI	GGCGCC	1 cut(s)	571
ErhI	CCWWGG	2 cut(s)	219, 534
Esp3I	CGTCTC	2 cut(s)	48, 656
FaeI	CATG	2 cut(s)	181, 223
FaiI	YATR	8 cut(s)	84, 141, 179, 221, 281, 503, 505, 602
FaqI	GGGAC	1 cut(s)	472
FatI	CATG	2 cut(s)	177, 219
FauI	CCCGC	2 cut(s)	194, 710
FauNDI	CATATG	1 cut(s)	503
Fnu4HI	GCNGC	9 cut(s)	246, 300, 305, 405, 541, 553, 568, 573, 689
FriOI	GRGCYC	2 cut(s)	288, 743
Fsp4HI	GCNGC	9 cut(s)	246, 300, 305, 405, 541, 553, 568, 573, 689
GlaI	GCGC	5 cut(s)	303, 551, 571, 598, 687
GluI	GCNGC	9 cut(s)	246, 300, 305, 405, 541, 553, 568, 573, 689
HaeII	RGCGCY	5 cut(s)	305, 553, 573, 600, 689
HaeIII	GGCC	7 cut(s)	224, 365, 383, 416, 426, 543, 651
HapII	CCGG	6 cut(s)	36, 308, 408, 525, 683, 720
HgaI	GACGC	1 cut(s)	107
HhaI	GCGC	5 cut(s)	304, 552, 572, 599, 688
Hin1I	GRCGYC	1 cut(s)	570
Hin1II	CATG	2 cut(s)	181, 223
Hin6I	GCGC	5 cut(s)	302, 550, 570, 597, 686
HinP1I	GCGC	5 cut(s)	302, 550, 570, 597, 686
HincII	GTYRAC	1 cut(s)	627
HindII	GTYRAC	1 cut(s)	627
HinfI	GANTC	1 cut(s)	618
HpaII	CCGG	6 cut(s)	36, 308, 408, 525, 683, 720
HphI	GGTGA	1 cut(s)	790
Hpy166II	GTNNAC	4 cut(s)	96, 122, 432, 627
Hpy188I	TCNGA	2 cut(s)	371, 613
Hpy188III	TCNNGA	3 cut(s)	58, 480, 520
Hpy8I	GTNNAC	4 cut(s)	96, 122, 432, 627
Hpy99I	CGWCG	2 cut(s)	422, 754
HpyAV	CCTTC	4 cut(s)	285, 416, 477, 486
HpyCH4IV	ACGT	3 cut(s)	293, 434, 665
HpyCH4V	TGCA	1 cut(s)	10
HpyF3I	CTNAG	2 cut(s)	317, 612
HpySE526I	ACGT	3 cut(s)	293, 434, 665
Hsp92I	GRCGYC	1 cut(s)	570
Hsp92II	CATG	2 cut(s)	181, 223
HspAI	GCGC	5 cut(s)	302, 550, 570, 597, 686
KasI	GGCGCC	1 cut(s)	569
KroI	GCCGGC	2 cut(s)	307, 682
KroNI	GCCGGC	2 cut(s)	309, 684
KspI	CCGCGG	1 cut(s)	190
Kzo9I	GATC	4 cut(s)	38, 258, 410, 578
LmnI	GCTCC	3 cut(s)	283, 503, 771
Lsp1109I	GCAGC	4 cut(s)	311, 391, 539, 675
MaeII	ACGT	3 cut(s)	293, 434, 665
MalI	GATC	4 cut(s)	40, 260, 412, 580
MboI	GATC	4 cut(s)	38, 258, 410, 578
MboII	GAAGA	3 cut(s)	345, 703, 766
MflI	RGATCY	1 cut(s)	578
MhlI	GDGCHC	4 cut(s)	288, 332, 500, 743
MlsI	TGGCCA	1 cut(s)	224
MluCI	AATT	1 cut(s)	814
MluNI	TGGCCA	1 cut(s)	224
Mly113I	GGCGCC	1 cut(s)	570
MlyI	GAGTC	1 cut(s)	627
Mox20I	TGGCCA	1 cut(s)	224
MroNI	GCCGGC	2 cut(s)	307, 682
MscI	TGGCCA	1 cut(s)	224
MslI	CAYNNNNRTG	1 cut(s)	561
Msp20I	TGGCCA	1 cut(s)	224
MspA1I	CMGCKG	1 cut(s)	189
MspI	CCGG	6 cut(s)	36, 308, 408, 525, 683, 720
MspR9I	CCNGG	2 cut(s)	362, 763
MvaI	CCWGG	2 cut(s)	362, 763
MvnI	CGCG	3 cut(s)	189, 638, 705
NaeI	GCCGGC	2 cut(s)	309, 684
NarI	GGCGCC	1 cut(s)	570
NcoI	CCATGG	1 cut(s)	219
NdeI	CATATG	1 cut(s)	503
NdeII	GATC	4 cut(s)	38, 258, 410, 578
NgoMIV	GCCGGC	2 cut(s)	307, 682
NlaIII	CATG	2 cut(s)	181, 223
NlaIV	GGNNCC	5 cut(s)	285, 488, 571, 580, 767
PaeR7I	CTCGAG	2 cut(s)	56, 70
PcsI	WCGNNNNNNNCGW	1 cut(s)	720
PdiI	GCCGGC	2 cut(s)	309, 684
PinAI	ACCGGT	1 cut(s)	524
PkrI	GCNGC	9 cut(s)	247, 301, 306, 406, 542, 554, 569, 574, 690
PleI	GAGTC	1 cut(s)	626
PluTI	GGCGCC	1 cut(s)	573
PpsI	GAGTC	1 cut(s)	626
PpuMI	RGGWCCY	1 cut(s)	486
Psp5II	RGGWCCY	1 cut(s)	486
Psp6I	CCWGG	2 cut(s)	360, 761
PspGI	CCWGG	2 cut(s)	360, 761
PspN4I	GGNNCC	5 cut(s)	285, 488, 571, 580, 767
PspPI	GGNCC	5 cut(s)	96, 364, 425, 486, 805
PspPPI	RGGWCCY	1 cut(s)	486
PspXI	VCTCGAGB	1 cut(s)	70
PsuI	RGATCY	1 cut(s)	578
RsaI	GTAC	1 cut(s)	158
RsaNI	GTAC	1 cut(s)	157
RseI	CAYNNNNRTG	1 cut(s)	561
SacII	CCGCGG	1 cut(s)	190
SatI	GCNGC	9 cut(s)	246, 300, 305, 405, 541, 553, 568, 573, 689
Sau3AI	GATC	4 cut(s)	38, 258, 410, 578
Sau96I	GGNCC	5 cut(s)	96, 364, 425, 486, 805
SchI	GAGTC	1 cut(s)	627
ScrFI	CCNGG	2 cut(s)	362, 763
SduI	GDGCHC	4 cut(s)	288, 332, 500, 743
SfoI	GGCGCC	1 cut(s)	571
Sfr274I	CTCGAG	2 cut(s)	56, 70
Sfr303I	CCGCGG	1 cut(s)	190
SgrBI	CCGCGG	1 cut(s)	190
SinI	GGWCC	3 cut(s)	96, 486, 805
SlaI	CTCGAG	2 cut(s)	56, 70
SmiMI	CAYNNNNRTG	1 cut(s)	561
SmlI	CTYRAG	2 cut(s)	56, 70
SmoI	CTYRAG	2 cut(s)	56, 70
Sse9I	AATT	1 cut(s)	814
SspDI	GGCGCC	1 cut(s)	569
StyD4I	CCNGG	2 cut(s)	360, 761
StyI	CCWWGG	2 cut(s)	219, 534
TaiI	ACGT	3 cut(s)	296, 437, 668
TaqI	TCGA	4 cut(s)	57, 71, 87, 420
TasI	AATT	1 cut(s)	814
TauI	GCSGC	5 cut(s)	248, 307, 543, 570, 575
TseI	GCWGC	4 cut(s)	299, 404, 552, 688
VpaK11BI	GGWCC	3 cut(s)	96, 486, 805
XhoI	CTCGAG	2 cut(s)	56, 70

Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.