Basic Information

Type: gene

Biological Identity

Species

rosa_samantha

Chromosome

Chr6C

Orthogroup ID

OG0007703

Physical Location & Seq

Genomic Coordinates Reverse (-)

66330065 .. 66332419

Gene Structure 2355 bp

Loading structure...

UTR

Exon/CDS

Intron

Transcript / Protein ID

Rh6CG499000.1

Sequence Viewer

CDS Protein Promoter (2k)

Length: 801 bp

ATGGCCCTCGAAGCTCTTAACTCTCCGACTGCCGCCGGCCAAGGCTTCCACTACGACGAGCCCAAGCTCCAGTACTCGGAGCCATGGACCAAGCGCAAGCGCTCCAAGCGTCCACGAAGCGTAGAGCAGACTCAAGTCTCTGAGGAGGAGTATCTCGCTCTCTGCCTCATCATGCTCGCTCGCGGCGGTAACAGCGGAGGACCCATCACCGCCTCACACCGCAGTCAGTCTCCGGTTCCAAGTGTTTCTCCTGTTATTGAACAGGCCACGTCAGCACCGGCCAAGGAGAATCAGCTGCTCAGTTACAAGTGCTCTGTTTGTGACAAGACTTTTCCCTCGTACCAGGCTCTGGGTGGACACAAGGCCAGCCACAGGAAGGGATCTTCCGCCGTCACCGGCGGCGAAGGACCCTCCACGTCATCCACCACCACCACCACATCCACCGTCACCGTCTCCAACGCCTCCGGTAGGTCCCACGAGTGCTCCATCTGCCACAAGTCTTTCCCCACCGGCCAGGCATTGGGAGGACACAAGCGGTGCCACTACGAGGGCGGCACCACCACCACCAGGTCCGCCGCAACCACCAGCGCCGTGACCACCTCGGAGGGAGTGGGGTCTACCACTCACACCGTCAGCCAGAGCCACCAACACCGCGAAGCGTTTGATCTGAACTTACCGGCCTTCCCGGAGCCGTTGTCAAGGAATTTCTTCCTCTCCGGCGACGACGAGGTGGAGAGCCCACATCCGACGAAGAAGCCCCGTCTATTCATGACCCCTAAAGTTGAGATATCACTCAATTAG

Functional Annotation

Gene Ontology

Molecular Function
Biological Process
Cellular Component

GO:0000003 GO:0001067 GO:0001101 GO:0003006 GO:0003674 GO:0003676 GO:0003677 GO:0003700 GO:0005488 GO:0005575 GO:0005622 GO:0005623 GO:0005634 GO:0006355 GO:0006950 GO:0006970 GO:0006972 GO:0006979 GO:0007275 GO:0008150 GO:0008152 GO:0009266 GO:0009314 GO:0009409 GO:0009414 GO:0009415 GO:0009416 GO:0009611 GO:0009628 GO:0009642 GO:0009644 GO:0009651 GO:0009719 GO:0009725 GO:0009737 GO:0009790 GO:0009791 GO:0009793 GO:0009889 GO:0009890 GO:0009892 GO:0009987 GO:0010033 GO:0010035 GO:0010117 GO:0010154 GO:0010200 GO:0010243 GO:0010468 GO:0010556 GO:0010558 GO:0010605 GO:0010629 GO:0015979 GO:0019219 GO:0019222 GO:0022414 GO:0031323 GO:0031324 GO:0031326 GO:0031327 GO:0032501 GO:0032502 GO:0033993 GO:0035264 GO:0040007 GO:0042221 GO:0042493 GO:0042538 GO:0043226 GO:0043227 GO:0043229 GO:0043231 GO:0043565 GO:0044212 GO:0044237 GO:0044424 GO:0044464 GO:0045892 GO:0045934 GO:0048316 GO:0048519 GO:0048523 GO:0048589 GO:0048608 GO:0048731 GO:0048856 GO:0050789 GO:0050794 GO:0050896 GO:0051171 GO:0051172 GO:0051252 GO:0051253 GO:0060255 GO:0061458 GO:0065007 GO:0080090 GO:0097159 GO:0097305 GO:0140110 GO:1901363 GO:1901698 GO:1901700 GO:1902679 GO:1903506 GO:1903507 GO:2000112 GO:2000113 GO:2001141

KEGG Pathways

Metabolic & Signaling

No pathways identified.

Pfam Domains

Protein Families

zf-C2H2_6

Protein Analysis

266

Amino Acids

28.45

Weight (kDa)

8.71

Isoelectric Point (pI)

68.63

Instability Index

Protein Domains (Pfam)

Domain Name	Pfam ID	Position	E-value	Description
zf-C2H2_6	PF13912	102 - 126	5.5e-14	C2H2-type zinc finger
zf-C2H2_6	PF13912	159 - 182	7.4e-12	C2H2-type zinc finger

Hydrophobicity Profile (Kyte-Doolittle)

AI Structure Prediction Report

Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter

Style Settings

Font Size (px)

Line Width

Align Labels (Cladogram)

Image

Tree File

Tip: Beautify your tree with professional tools

Download the Full Tree (.nwk) file, then upload it to any of the following tools to customize colors, fonts, annotations, clades, and branch support.

Publication-ready

iTOL Interactive Tree Of Life · Most popular EvolView Flexible annotations MEGA Desktop phylogenetics suite FigTree Desktop tree editor

Orthologous Genes (Group: OG0007703)

Species	Orthologous Gene IDs
arabidopsis_thaliana	AT1G27730 AT3G49930 AT5G04340 AT5G67450
fragaria_vesca	FvH4_2g33250
malus_domestica	MD01G1041900.v1.1 MD03G1122300.v1.1 MD08G1086500.v1.1 MD15G1071400.v1.1
prunus_persica	Prupe.1G424300_v2.0.a1
pyrus_communis	pycom08g07100 pycom15g06720
rosa_chinensis	RchiOBHm_Chr6g0309391
rosa_laevigata	RLG00000010570
rosa_multiflora	Rmu_co8321009.1_g000001 Rmu_sc0012466.1_g000004 Rmu_sc0017306.1_g000001
rosa_roxburghii	Rroxscaffold_7G00159440
rosa_rugosa	Rorug06G0371800
rosa_samantha	Rh6AG484900 Rh6BG494100 Rh6CG499000 Rh6DG485300
rosa_wichuraiana	Rw6G042230

Restriction Enzyme Sites

1 / 10

Enzyme	Recognition Site	Cut Count	Positions (bp)
AccB1I	GGYRCC	2 cut(s)	537, 554
AccB7I	CCANNNNNTGG	2 cut(s)	349, 520
AccI	GTMKAC	1 cut(s)	617
AccII	CGCG	2 cut(s)	183, 654
AclWI	GGATC	1 cut(s)	388
AcoI	YGGCCR	3 cut(s)	37, 279, 511
AcsI	RAATTY	1 cut(s)	703
AfaI	GTAC	2 cut(s)	74, 341
AfeI	AGCGCT	1 cut(s)	101
AfiI	CCNNNNNNNGG	8 cut(s)	76, 349, 372, 376, 468, 520, 547, 567
AgsI	TTSAA	1 cut(s)	260
AjiI	CACGTC	2 cut(s)	270, 417
AjnI	CCWGG	3 cut(s)	342, 513, 566
AluBI	AGCT	3 cut(s)	14, 67, 295
AluI	AGCT	3 cut(s)	14, 67, 295
Alw21I	GWGCWC	2 cut(s)	314, 485
Alw26I	GTCTC	3 cut(s)	142, 234, 457
AlwI	GGATC	1 cut(s)	388
AlwNI	CAGNNNCTG	1 cut(s)	349
Aor51HI	AGCGCT	1 cut(s)	101
AoxI	GGCC	7 cut(s)	3, 37, 264, 279, 363, 511, 678
ApeKI	GCWGC	1 cut(s)	295
ApoI	RAATTY	1 cut(s)	703
Asp700I	GAANNNNTTC	1 cut(s)	707
AspLEI	GCGC	3 cut(s)	96, 102, 590
AspS9I	GGNCC	6 cut(s)	4, 87, 200, 407, 471, 570
AsuC2I	CCSGG	1 cut(s)	686
AsuHPI	GGTGA	3 cut(s)	199, 385, 439
AvaII	GGWCC	5 cut(s)	87, 200, 407, 471, 570
BanI	GGYRCC	2 cut(s)	537, 554
BanII	GRGCYC	2 cut(s)	63, 740
BauI	CACGAG	1 cut(s)	476
Bbv12I	GWGCWC	2 cut(s)	314, 485
BbvI	GCAGC	1 cut(s)	282
BccI	CCATC	2 cut(s)	212, 494
BceAI	ACGGC	3 cut(s)	374, 575, 676
BciT130I	CCWGG	3 cut(s)	344, 515, 568
BcnI	CCSGG	1 cut(s)	686
BcoDI	GTCTC	3 cut(s)	142, 234, 457
BfoI	RGCGCY	2 cut(s)	103, 591
BisI	GCNGC	6 cut(s)	33, 184, 296, 400, 553, 576
BlsI	GCNGC	6 cut(s)	34, 185, 297, 401, 554, 577
BmcAI	AGTACT	1 cut(s)	74
Bme1390I	CCNGG	4 cut(s)	344, 515, 568, 686
Bme18I	GGWCC	5 cut(s)	87, 200, 407, 471, 570
BmgBI	CACGTC	2 cut(s)	270, 417
BmgT120I	GGNCC	6 cut(s)	4, 87, 200, 407, 471, 570
BmiI	GGNNCC	8 cut(s)	81, 202, 237, 409, 473, 539, 556, 690
BmrFI	CCNGG	4 cut(s)	344, 515, 568, 686
BoxI	GACNNNNGTC	1 cut(s)	134
BpmI	CTGGAG	1 cut(s)	53
BpuEI	CTTGAG	1 cut(s)	117
BpuMI	CCSGG	1 cut(s)	686
BsaJI	CCNNGG	4 cut(s)	40, 83, 282, 600
BsaWI	WCCGGW	2 cut(s)	232, 464
BsaXI	ACNNNNNCTCC	4 cut(s)	467, 497, 680, 710
Bsc4I	CCNNNNNNNGG	8 cut(s)	76, 349, 372, 376, 468, 520, 547, 567
Bse118I	RCCGGY	5 cut(s)	35, 277, 395, 509, 676
Bse1I	ACTGG	1 cut(s)	70
BseBI	CCWGG	3 cut(s)	344, 515, 568
BseDI	CCNNGG	4 cut(s)	40, 83, 282, 600
BseGI	GGATG	3 cut(s)	419, 437, 742
BseLI	CCNNNNNNNGG	8 cut(s)	76, 349, 372, 376, 468, 520, 547, 567
BseMII	CTCAG	2 cut(s)	132, 313
BseNI	ACTGG	1 cut(s)	70
BseRI	GAGGAG	2 cut(s)	158, 161
BseXI	GCAGC	1 cut(s)	282
Bsh1236I	CGCG	2 cut(s)	183, 654
BshFI	GGCC	7 cut(s)	5, 39, 266, 281, 365, 513, 680
BshNI	GGYRCC	2 cut(s)	537, 554
BsiHKAI	GWGCWC	2 cut(s)	314, 485
BsiSI	CCGG	9 cut(s)	36, 233, 278, 396, 465, 510, 677, 686, 717
BslFI	GGGAC	1 cut(s)	457
BslI	CCNNNNNNNGG	8 cut(s)	76, 349, 372, 376, 468, 520, 547, 567
BsmAI	GTCTC	3 cut(s)	142, 234, 457
BsmBI	CGTCTC	1 cut(s)	457
BsmFI	GGGAC	1 cut(s)	457
BsnI	GGCC	7 cut(s)	5, 39, 266, 281, 365, 513, 680
Bsp1286I	GDGCHC	4 cut(s)	63, 314, 485, 740
Bsp143I	GATC	2 cut(s)	380, 664
Bsp19I	CCATGG	1 cut(s)	83
BspANI	GGCC	7 cut(s)	5, 39, 266, 281, 365, 513, 680
BspCNI	CTCAG	2 cut(s)	133, 312
BspFNI	CGCG	2 cut(s)	183, 654
BspHI	TCATGA	1 cut(s)	768
BspLI	GGNNCC	8 cut(s)	81, 202, 237, 409, 473, 539, 556, 690
BspPI	GGATC	1 cut(s)	388
BspT107I	GGYRCC	2 cut(s)	537, 554
BsrFI	RCCGGY	5 cut(s)	35, 277, 395, 509, 676
BsrI	ACTGG	1 cut(s)	70
BssAI	RCCGGY	5 cut(s)	35, 277, 395, 509, 676
BssECI	CCNNGG	4 cut(s)	40, 83, 282, 600
BssMI	GATC	2 cut(s)	380, 664
BssSI	CACGAG	1 cut(s)	476
BssT1I	CCWWGG	3 cut(s)	40, 83, 282
Bst2BI	CACGAG	1 cut(s)	476
Bst2UI	CCWGG	3 cut(s)	344, 515, 568
Bst4CI	ACNGT	3 cut(s)	445, 451, 631
BstC8I	GCNNGC	5 cut(s)	37, 98, 177, 181, 367
BstDEI	CTNAG	2 cut(s)	141, 299
BstDSI	CCRYGG	1 cut(s)	83
BstENI	CCTNNNNNAGG	1 cut(s)	466
BstF5I	GGATG	3 cut(s)	419, 437, 742
BstFNI	CGCG	2 cut(s)	183, 654
BstH2I	RGCGCY	2 cut(s)	103, 591
BstHHI	GCGC	3 cut(s)	96, 102, 590
BstKTI	GATC	2 cut(s)	383, 667
BstMAI	GTCTC	3 cut(s)	142, 234, 457
BstMBI	GATC	2 cut(s)	380, 664
BstMWI	GCNNNNNNNGC	5 cut(s)	11, 106, 192, 272, 489
BstNI	CCWGG	3 cut(s)	344, 515, 568
BstPAI	GACNNNNGTC	1 cut(s)	134
BstSCI	CCNGG	4 cut(s)	342, 513, 566, 684
BstUI	CGCG	2 cut(s)	183, 654
BstV1I	GCAGC	1 cut(s)	282
BstX2I	RGATCY	1 cut(s)	380
BstYI	RGATCY	1 cut(s)	380
BsuRI	GGCC	7 cut(s)	5, 39, 266, 281, 365, 513, 680
BtgI	CCRYGG	1 cut(s)	83
BtrI	CACGTC	2 cut(s)	270, 417
BtsCI	GGATG	3 cut(s)	419, 437, 742
Cac8I	GCNNGC	5 cut(s)	37, 98, 177, 181, 367
CaiI	CAGNNNCTG	1 cut(s)	349
CciI	TCATGA	1 cut(s)	768
CfoI	GCGC	3 cut(s)	96, 102, 590
Cfr10I	RCCGGY	5 cut(s)	35, 277, 395, 509, 676
Cfr13I	GGNCC	6 cut(s)	4, 87, 200, 407, 471, 570
CseI	GACGC	1 cut(s)	98
CsiI	ACCWGGT	1 cut(s)	566
Csp6I	GTAC	2 cut(s)	73, 340
CviAII	CATG	3 cut(s)	84, 172, 769
CviQI	GTAC	2 cut(s)	73, 340
DdeI	CTNAG	2 cut(s)	141, 299
DpnI	GATC	2 cut(s)	382, 666
DpnII	GATC	2 cut(s)	380, 664
EaeI	YGGCCR	3 cut(s)	37, 279, 511
EciI	GGCGGA	2 cut(s)	376, 562
Eco130I	CCWWGG	3 cut(s)	40, 83, 282
Eco24I	GRGCYC	2 cut(s)	63, 740
Eco32I	GATATC	1 cut(s)	789
Eco47I	GGWCC	5 cut(s)	87, 200, 407, 471, 570
Eco47III	AGCGCT	1 cut(s)	101
EcoNI	CCTNNNNNAGG	1 cut(s)	466
EcoO109I	RGGNCCY	3 cut(s)	200, 407, 471
EcoRII	CCWGG	3 cut(s)	342, 513, 566
EcoRV	GATATC	1 cut(s)	789
EcoT14I	CCWWGG	3 cut(s)	40, 83, 282
EcoT38I	GRGCYC	2 cut(s)	63, 740
ErhI	CCWWGG	3 cut(s)	40, 83, 282
Esp3I	CGTCTC	1 cut(s)	457
FaeI	CATG	3 cut(s)	87, 175, 772
FaiI	YATR	3 cut(s)	85, 173, 770
FaqI	GGGAC	1 cut(s)	457
FatI	CATG	3 cut(s)	83, 171, 768
FblI	GTMKAC	1 cut(s)	617
Fnu4HI	GCNGC	6 cut(s)	33, 184, 296, 400, 553, 576
FokI	GGATG	3 cut(s)	406, 424, 729
FriOI	GRGCYC	2 cut(s)	63, 740
Fsp4HI	GCNGC	6 cut(s)	33, 184, 296, 400, 553, 576
GlaI	GCGC	3 cut(s)	95, 101, 589
GluI	GCNGC	6 cut(s)	33, 184, 296, 400, 553, 576
GsuI	CTGGAG	1 cut(s)	53
HaeII	RGCGCY	2 cut(s)	103, 591
HaeIII	GGCC	7 cut(s)	5, 39, 266, 281, 365, 513, 680
HapII	CCGG	9 cut(s)	36, 233, 278, 396, 465, 510, 677, 686, 717
HgaI	GACGC	1 cut(s)	98
HhaI	GCGC	3 cut(s)	96, 102, 590
Hin1II	CATG	3 cut(s)	87, 175, 772
Hin6I	GCGC	3 cut(s)	94, 100, 588
HinP1I	GCGC	3 cut(s)	94, 100, 588
HinfI	GANTC	2 cut(s)	130, 289
HpaII	CCGG	9 cut(s)	36, 233, 278, 396, 465, 510, 677, 686, 717
HphI	GGTGA	3 cut(s)	199, 385, 439
Hpy166II	GTNNAC	3 cut(s)	113, 356, 618
Hpy188I	TCNGA	6 cut(s)	27, 79, 142, 604, 669, 747
Hpy188III	TCNNGA	1 cut(s)	769
Hpy8I	GTNNAC	3 cut(s)	113, 356, 618
Hpy99I	CGWCG	4 cut(s)	59, 725, 728, 751
HpyAV	CCTTC	3 cut(s)	370, 398, 691
HpyCH4III	ACNGT	3 cut(s)	445, 451, 631
HpyCH4IV	ACGT	2 cut(s)	269, 416
HpyF10VI	GCNNNNNNNGC	5 cut(s)	11, 106, 192, 272, 489
HpyF3I	CTNAG	2 cut(s)	141, 299
HpySE526I	ACGT	2 cut(s)	269, 416
Hsp92II	CATG	3 cut(s)	87, 175, 772
HspAI	GCGC	3 cut(s)	94, 100, 588
KroI	GCCGGC	1 cut(s)	35
KroNI	GCCGGC	1 cut(s)	37
Kzo9I	GATC	2 cut(s)	380, 664
LmnI	GCTCC	5 cut(s)	72, 79, 107, 488, 688
Lsp1109I	GCAGC	1 cut(s)	282
MabI	ACCWGGT	1 cut(s)	566
MaeII	ACGT	2 cut(s)	269, 416
MaeIII	GTNAC	6 cut(s)	188, 302, 320, 391, 445, 592
MalI	GATC	2 cut(s)	382, 666
MboI	GATC	2 cut(s)	380, 664
MboII	GAAGA	3 cut(s)	375, 700, 763
MflI	RGATCY	1 cut(s)	380
MhlI	GDGCHC	4 cut(s)	63, 314, 485, 740
MluCI	AATT	2 cut(s)	703, 796
MlyI	GAGTC	1 cut(s)	124
MmeI	TCCRAC	3 cut(s)	50, 480, 770
MroNI	GCCGGC	1 cut(s)	35
MroXI	GAANNNNTTC	1 cut(s)	707
MseI	TTAA	1 cut(s)	18
MspA1I	CMGCKG	2 cut(s)	195, 295
MspI	CCGG	9 cut(s)	36, 233, 278, 396, 465, 510, 677, 686, 717
MspR9I	CCNGG	4 cut(s)	344, 515, 568, 686
MvaI	CCWGG	3 cut(s)	344, 515, 568
MvnI	CGCG	2 cut(s)	183, 654
MwoI	GCNNNNNNNGC	5 cut(s)	11, 106, 192, 272, 489
NaeI	GCCGGC	1 cut(s)	37
NciI	CCSGG	1 cut(s)	686
NcoI	CCATGG	1 cut(s)	83
NdeII	GATC	2 cut(s)	380, 664
NgoMIV	GCCGGC	1 cut(s)	35
NlaIII	CATG	3 cut(s)	87, 175, 772
NlaIV	GGNNCC	8 cut(s)	81, 202, 237, 409, 473, 539, 556, 690
NmuCI	GTSAC	4 cut(s)	320, 391, 445, 592
PagI	TCATGA	1 cut(s)	768
PdiI	GCCGGC	1 cut(s)	37
PdmI	GAANNNNTTC	1 cut(s)	707
PfeI	GAWTC	1 cut(s)	289
PflMI	CCANNNNNTGG	2 cut(s)	349, 520
PfoI	TCCNGGA	1 cut(s)	684
PkrI	GCNGC	6 cut(s)	34, 185, 297, 401, 554, 577
PleI	GAGTC	1 cut(s)	124
PpsI	GAGTC	1 cut(s)	124
PpuMI	RGGWCCY	3 cut(s)	200, 407, 471
PshAI	GACNNNNGTC	1 cut(s)	134
Psp5II	RGGWCCY	3 cut(s)	200, 407, 471
Psp6I	CCWGG	3 cut(s)	342, 513, 566
PspGI	CCWGG	3 cut(s)	342, 513, 566
PspN4I	GGNNCC	8 cut(s)	81, 202, 237, 409, 473, 539, 556, 690
PspPI	GGNCC	6 cut(s)	4, 87, 200, 407, 471, 570
PspPPI	RGGWCCY	3 cut(s)	200, 407, 471
PstNI	CAGNNNCTG	1 cut(s)	349
PsuI	RGATCY	1 cut(s)	380
PvuII	CAGCTG	1 cut(s)	295
RsaI	GTAC	2 cut(s)	74, 341
RsaNI	GTAC	2 cut(s)	73, 340
SaqAI	TTAA	1 cut(s)	18
SatI	GCNGC	6 cut(s)	33, 184, 296, 400, 553, 576
Sau3AI	GATC	2 cut(s)	380, 664
Sau96I	GGNCC	6 cut(s)	4, 87, 200, 407, 471, 570
ScaI	AGTACT	1 cut(s)	74
SchI	GAGTC	1 cut(s)	124
ScrFI	CCNGG	4 cut(s)	344, 515, 568, 686
SduI	GDGCHC	4 cut(s)	63, 314, 485, 740
SetI	ASST	9 cut(s)	16, 69, 272, 297, 419, 473, 572, 602, 732
SexAI	ACCWGGT	1 cut(s)	566
SgrAI	CRCCGGYG	1 cut(s)	395
SinI	GGWCC	5 cut(s)	87, 200, 407, 471, 570
SmlI	CTYRAG	1 cut(s)	132
SmoI	CTYRAG	1 cut(s)	132
Sse9I	AATT	2 cut(s)	703, 796
StyD4I	CCNGG	4 cut(s)	342, 513, 566, 684
StyI	CCWWGG	3 cut(s)	40, 83, 282
TaaI	ACNGT	3 cut(s)	445, 451, 631
TaiI	ACGT	2 cut(s)	272, 419
TaqI	TCGA	1 cut(s)	9
TasI	AATT	2 cut(s)	703, 796
TatI	WGTACW	1 cut(s)	72
TauI	GCSGC	5 cut(s)	35, 186, 402, 555, 578
TfiI	GAWTC	1 cut(s)	289
Tru1I	TTAA	1 cut(s)	18
Tru9I	TTAA	1 cut(s)	18
TseFI	GTSAC	4 cut(s)	320, 391, 445, 592
TseI	GCWGC	1 cut(s)	295
Tsp45I	GTSAC	4 cut(s)	320, 391, 445, 592
TspDTI	ATGAA	1 cut(s)	757
Van91I	CCANNNNNTGG	2 cut(s)	349, 520
VpaK11BI	GGWCC	5 cut(s)	87, 200, 407, 471, 570
XagI	CCTNNNNNAGG	1 cut(s)	466
XapI	RAATTY	1 cut(s)	703
XcmI	CCANNNNNNNNNTGG	1 cut(s)	350
XmiI	GTMKAC	1 cut(s)	617
XmnI	GAANNNNTTC	1 cut(s)	707
ZrmI	AGTACT	1 cut(s)	74

Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.