pycom08g08010

BOI-related E3 ubiquitin-protein ligase

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
pyrus_communis
Chr8
OG0010150
Physical Location & Seq
Reverse (-)
6466257 .. 6467916
1660 bp
Loading structure...
UTR
Exon/CDS
Intron
N/A

Sequence Viewer

Length: 528 bp
ATGGGTGACCGACTGCGGCAAACTATCATGGACAAGGTCCTAGCCACACAACTTCAGACCCTCTCGGCTGTGGAAGACAAAGTTCATCGGAAGCTGCGAGAGAAAGAAGCAGAAGTGGAGAGCATTAACGAGAAGAATATGGAGCTTGAAGAGCAAATGGAACAGTTGACTGTTGAAGCAGGTGCTTGGCAACAGCTAGCCAGGCGCAATGAAAACATGATCTCAACTCTAAGGTTTAATCTTCAGCATGTATATGCTCAAAGTAGAGATAGTAAAGAAGGATGCGGTGACAGTGAGGTAGATGATACAGCTTCTTGCTGCAATGGCCGATCGATCAATTTGGAAATGTGTTGCAAGGAGAACAATGACGGGAAAGAGATGATGACTTGCAAGGCCTGCCGAGTCAATGAAGTGTGCATGCTTCTATTACCTTGCAAGCATCTTTGCCTGTGTAAAGATTGTGAAACTAAGCTCAGCATCTGCCCTCTCTGTCAGTCCTCTAAGTTTATCGGCATGGAGATCGACTGA
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
GO:0005575 GO:0005622 GO:0005623 GO:0005737 GO:0044424 GO:0044464
KEGG Pathways
Metabolic & Signaling
No pathways identified.
Pfam Domains
Protein Families
zf-C3HC4_3

Protein Analysis

176

Amino Acids

19.93

Weight (kDa)

5.13

Isoelectric Point (pI)

45.5

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
zf-C3HC4_3 PF13920 128 - 166 1.1e-08 Zinc finger, C3HC4 type (RING finger)
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AarI CACCTGC 1 cut(s) 170
Acc36I ACCTGC 1 cut(s) 170
AciI CCGC 2 cut(s) 16, 285
AcoI YGGCCR 1 cut(s) 325
AcuI CTGAAG 2 cut(s) 38, 227
AgsI TTSAA 2 cut(s) 149, 176
AjnI CCWGG 1 cut(s) 200
AluBI AGCT 5 cut(s) 94, 145, 196, 311, 472
AluI AGCT 5 cut(s) 94, 145, 196, 311, 472
AlwNI CAGNNNCTG 1 cut(s) 480
AoxI GGCC 2 cut(s) 325, 393
ApeKI GCWGC 2 cut(s) 94, 318
AspLEI GCGC 1 cut(s) 207
AspS9I GGNCC 1 cut(s) 37
AsuHPI GGTGA 2 cut(s) 17, 299
AsuNHI GCTAGC 1 cut(s) 196
AvaII GGWCC 1 cut(s) 37
BbsI GAAGAC 1 cut(s) 81
BbvI GCAGC 2 cut(s) 81, 305
BcgI CGANNNNNNTGC 1 cut(s) 502
BciT130I CCWGG 1 cut(s) 202
BfaI CTAG 2 cut(s) 41, 197
BfuAI ACCTGC 1 cut(s) 170
BisI GCNGC 3 cut(s) 17, 95, 319
BlpI GCTNAGC 1 cut(s) 473
BlsI GCNGC 3 cut(s) 18, 96, 320
Bme1390I CCNGG 1 cut(s) 202
Bme18I GGWCC 1 cut(s) 37
BmgT120I GGNCC 1 cut(s) 37
BmrFI CCNGG 1 cut(s) 202
BmsI GCATC 3 cut(s) 272, 448, 486
BmtI GCTAGC 1 cut(s) 200
BpiI GAAGAC 1 cut(s) 81
Bpu1102I GCTNAGC 1 cut(s) 473
Bsa29I ATCGAT 1 cut(s) 332
Bse3DI GCAATG 2 cut(s) 214, 328
BseBI CCWGG 1 cut(s) 202
BseCI ATCGAT 1 cut(s) 332
BseGI GGATG 1 cut(s) 287
BseMI GCAATG 2 cut(s) 214, 328
BseMII CTCAG 1 cut(s) 487
BseXI GCAGC 2 cut(s) 81, 305
Bsh1285I CGRYCG 1 cut(s) 332
BshFI GGCC 2 cut(s) 327, 395
BshVI ATCGAT 1 cut(s) 332
BsiEI CGRYCG 1 cut(s) 332
BsnI GGCC 2 cut(s) 327, 395
Bsp143I GATC 4 cut(s) 219, 329, 333, 519
Bsp1720I GCTNAGC 1 cut(s) 473
BspACI CCGC 2 cut(s) 16, 285
BspANI GGCC 2 cut(s) 327, 395
BspCNI CTCAG 1 cut(s) 486
BspDI ATCGAT 1 cut(s) 332
BspMI ACCTGC 1 cut(s) 170
BspOI GCTAGC 1 cut(s) 200
BspQI GCTCTTC 1 cut(s) 144
BsrDI GCAATG 2 cut(s) 214, 328
BssMI GATC 4 cut(s) 219, 329, 333, 519
Bst2UI CCWGG 1 cut(s) 202
Bst4CI ACNGT 3 cut(s) 165, 172, 293
Bst6I CTCTTC 1 cut(s) 144
BstAPI GCANNNNNTGC 1 cut(s) 396
BstC8I GCNNGC 4 cut(s) 198, 397, 419, 437
BstDEI CTNAG 4 cut(s) 230, 468, 473, 501
BstEII GGTNACC 1 cut(s) 5
BstF5I GGATG 1 cut(s) 287
BstHHI GCGC 1 cut(s) 207
BstKTI GATC 4 cut(s) 222, 332, 336, 522
BstMBI GATC 4 cut(s) 219, 329, 333, 519
BstMCI CGRYCG 1 cut(s) 332
BstMWI GCNNNNNNNGC 4 cut(s) 151, 202, 324, 396
BstNI CCWGG 1 cut(s) 202
BstNSI RCATGY 2 cut(s) 251, 421
BstPI GGTNACC 1 cut(s) 5
BstSCI CCNGG 1 cut(s) 200
BstV1I GCAGC 2 cut(s) 81, 305
BstV2I GAAGAC 1 cut(s) 81
Bsu15I ATCGAT 1 cut(s) 332
BsuRI GGCC 2 cut(s) 327, 395
BsuTUI ATCGAT 1 cut(s) 332
BtsCI GGATG 1 cut(s) 287
BtsIMutI CAGTG 1 cut(s) 298
BveI ACCTGC 1 cut(s) 170
Cac8I GCNNGC 4 cut(s) 198, 397, 419, 437
CaiI CAGNNNCTG 1 cut(s) 480
CfoI GCGC 1 cut(s) 207
Cfr13I GGNCC 1 cut(s) 37
ClaI ATCGAT 1 cut(s) 332
CviAII CATG 5 cut(s) 28, 217, 248, 418, 514
DdeI CTNAG 4 cut(s) 230, 468, 473, 501
DpnI GATC 4 cut(s) 221, 331, 335, 521
DpnII GATC 4 cut(s) 219, 329, 333, 519
EaeI YGGCCR 1 cut(s) 325
Eam1104I CTCTTC 1 cut(s) 144
EarI CTCTTC 1 cut(s) 144
Eco147I AGGCCT 1 cut(s) 395
Eco47I GGWCC 1 cut(s) 37
Eco57I CTGAAG 2 cut(s) 38, 227
Eco91I GGTNACC 1 cut(s) 5
EcoO109I RGGNCCY 1 cut(s) 37
EcoO65I GGTNACC 1 cut(s) 5
EcoRII CCWGG 1 cut(s) 200
FaeI CATG 5 cut(s) 31, 220, 251, 421, 517
FaiI YATR 8 cut(s) 29, 140, 218, 249, 253, 255, 419, 515
FatI CATG 5 cut(s) 27, 216, 247, 417, 513
Fnu4HI GCNGC 3 cut(s) 17, 95, 319
FokI GGATG 1 cut(s) 294
Fsp4HI GCNGC 3 cut(s) 17, 95, 319
FspBI CTAG 2 cut(s) 41, 197
GlaI GCGC 1 cut(s) 206
GluI GCNGC 3 cut(s) 17, 95, 319
HaeIII GGCC 2 cut(s) 327, 395
HhaI GCGC 1 cut(s) 207
Hin1II CATG 5 cut(s) 31, 220, 251, 421, 517
Hin6I GCGC 1 cut(s) 205
HinP1I GCGC 1 cut(s) 205
HincII GTYRAC 1 cut(s) 168
HindII GTYRAC 1 cut(s) 168
HinfI GANTC 1 cut(s) 402
HphI GGTGA 2 cut(s) 17, 299
Hpy166II GTNNAC 1 cut(s) 168
Hpy188I TCNGA 2 cut(s) 57, 90
Hpy8I GTNNAC 1 cut(s) 168
HpyAV CCTTC 1 cut(s) 272
HpyCH4III ACNGT 3 cut(s) 165, 172, 293
HpyCH4V TGCA 5 cut(s) 321, 354, 390, 417, 435
HpyF10VI GCNNNNNNNGC 4 cut(s) 151, 202, 324, 396
HpyF3I CTNAG 4 cut(s) 230, 468, 473, 501
Hsp92II CATG 5 cut(s) 31, 220, 251, 421, 517
HspAI GCGC 1 cut(s) 205
Kzo9I GATC 4 cut(s) 219, 329, 333, 519
LguI GCTCTTC 1 cut(s) 144
LmnI GCTCC 1 cut(s) 142
LpnPI CCDG 5 cut(s) 165, 187, 214, 409, 461
Lsp1109I GCAGC 2 cut(s) 81, 305
LweI GCATC 3 cut(s) 272, 448, 486
MaeI CTAG 2 cut(s) 41, 197
MaeIII GTNAC 2 cut(s) 5, 287
MalI GATC 4 cut(s) 221, 331, 335, 521
MboI GATC 4 cut(s) 219, 329, 333, 519
MboII GAAGA 4 cut(s) 86, 145, 161, 233
MluCI AATT 1 cut(s) 337
MlyI GAGTC 1 cut(s) 411
MnlI CCTC 4 cut(s) 71, 289, 495, 508
MseI TTAA 2 cut(s) 126, 237
MslI CAYNNNNRTG 1 cut(s) 252
MspR9I CCNGG 1 cut(s) 202
MvaI CCWGG 1 cut(s) 202
MwoI GCNNNNNNNGC 4 cut(s) 151, 202, 324, 396
NdeII GATC 4 cut(s) 219, 329, 333, 519
NheI GCTAGC 1 cut(s) 196
NlaIII CATG 5 cut(s) 31, 220, 251, 421, 517
NmeAIII GCCGAG 2 cut(s) 44, 425
NmuCI GTSAC 2 cut(s) 5, 287
NspI RCATGY 2 cut(s) 251, 421
PaeI GCATGC 1 cut(s) 421
PaqCI CACCTGC 1 cut(s) 170
PceI AGGCCT 1 cut(s) 395
PciSI GCTCTTC 1 cut(s) 144
PcsI WCGNNNNNNNCGW 1 cut(s) 94
PflFI GACNNNGTC 1 cut(s) 35
PkrI GCNGC 3 cut(s) 18, 96, 320
Ple19I CGATCG 1 cut(s) 332
PleI GAGTC 1 cut(s) 410
PpsI GAGTC 1 cut(s) 410
PpuMI RGGWCCY 1 cut(s) 37
Psp5II RGGWCCY 1 cut(s) 37
Psp6I CCWGG 1 cut(s) 200
PspEI GGTNACC 1 cut(s) 5
PspGI CCWGG 1 cut(s) 200
PspPI GGNCC 1 cut(s) 37
PspPPI RGGWCCY 1 cut(s) 37
PstNI CAGNNNCTG 1 cut(s) 480
PsyI GACNNNGTC 1 cut(s) 35
PvuI CGATCG 1 cut(s) 332
RseI CAYNNNNRTG 1 cut(s) 252
SapI GCTCTTC 1 cut(s) 144
SaqAI TTAA 2 cut(s) 126, 237
SatI GCNGC 3 cut(s) 17, 95, 319
Sau3AI GATC 4 cut(s) 219, 329, 333, 519
Sau96I GGNCC 1 cut(s) 37
SchI GAGTC 1 cut(s) 411
ScrFI CCNGG 1 cut(s) 202
SfaNI GCATC 3 cut(s) 272, 448, 486
SinI GGWCC 1 cut(s) 37
SmiMI CAYNNNNRTG 1 cut(s) 252
SphI GCATGC 1 cut(s) 421
Sse9I AATT 1 cut(s) 337
SseBI AGGCCT 1 cut(s) 395
SsiI CCGC 2 cut(s) 16, 285
SspMI CTAG 2 cut(s) 41, 197
StuI AGGCCT 1 cut(s) 395
StyD4I CCNGG 1 cut(s) 200
TaaI ACNGT 3 cut(s) 165, 172, 293
TaqI TCGA 2 cut(s) 332, 522
TaqII GACCGA 1 cut(s) 24
TasI AATT 1 cut(s) 337
TauI GCSGC 1 cut(s) 19
Tru1I TTAA 2 cut(s) 126, 237
Tru9I TTAA 2 cut(s) 126, 237
TscAI CASTG 1 cut(s) 298
TseFI GTSAC 2 cut(s) 5, 287
TseI GCWGC 2 cut(s) 94, 318
Tsp45I GTSAC 2 cut(s) 5, 287
TspDTI ATGAA 3 cut(s) 74, 225, 423
TspRI CASTG 1 cut(s) 298
Tth111I GACNNNGTC 1 cut(s) 35
VpaK11BI GGWCC 1 cut(s) 37
XceI RCATGY 2 cut(s) 251, 421
XspI CTAG 2 cut(s) 41, 197
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.