pycom08g09060

glycine-rich protein

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
pyrus_communis
Chr8
OG0002500
Physical Location & Seq
Forward (+)
7312808 .. 7321981
9174 bp
Loading structure...
UTR
Exon/CDS
Intron
N/A

Sequence Viewer

Length: 4350 bp
ATGGCTCGCTTCCGCACTCCCACTCTCCTCCTCGCATTCCTCTCCGCCCTTGTTGCCCTAACCGCAAACCCTAGCCACCTCCTCCTCGCGTCCGAAGATGAGGACTTTTCGTTTACCGATTCCTCCGATTCCGATTCCTATCTCTTCCACCAAGACTACTCGCCGCCGGCTCCGCCACCTCCTCCGCCGCTCCCGCCGTCGGTCTCTTGCACCGATGACCTCGGCGGCGTCGGCTCTCTGGACGCCACGTGTCAGATAGTCTCCGATTTGAACCTCACCGCCGACGTGTACATAACAGGGAAGGGCAACTTTTATATCCTGCCCGGGGTGAGATTCGGCTGCGCGATTCATGGCTGCGCGATAATTATTAACATTAGTGGTAACTTTAGCTTAGGCAGCAACGCGTCGCTTTTGGCCGGGGCTTTTGAGCTCACGGCGTGCAATGCGAGCTTCCTCAACGGCTCGGCGCTGAACACGACGGCTTTGGCTGGGAAGCCGCCGCCGCAGACGAGTGGAACTCCGCAGGGGATAGATGGGGCGGGTGGAGGACACGGAGGGCGGGGGGCGTGTTGTTTGGTGGACAAGACGAAGCTTCCGGAGGACGTGTGGGGAGGGGATGCATACTCGTGGTCGACGCTGCAGAGGCCGGCTAGTTTTGGGAGCCGAGGCGGGTCGACGAGTAAGGAGGTGGATTATGGAGGTTTAGGGGGAGGGAGGGTGAGGCTCCAAGTGAAGGAATTGCTGGTTGTGGAGGGAAGTGTTTTGGCTGAAGGTGGTGGCGGAGGGAATAGAGGTGGCGGTGGATCTGGTGGCAGTATCTACATTAAGGCTCATAGAATGACTGGTAGTGGCAGGATAAGTGCTTGTGGTGGTGATGGTTACGCTGGTGGTGGTGGTGGAAGAGTGTCAGTTGATGTCTATAGCAGGCACGATGACCCCAAAATTTTTGTGCATGGAGGAAATAGCTATGCTTGTCCAGAAAATGCAGGCGGGGCTGGGACTTTATATGATGCAGTTCCTCGAAGCCTTATTGTTAGCAATCATAACAGGTCAACAGATACAGAATCACTTCTTATGGAGTTTCCTTATCAGCCTCTTTGGACAAATGTTTATATTCAAAATAAGGCGAGGGCGACTGTCCCATTACTTTGGAGTCGTGTCCAGGTGCAAGGACAGATAAGCCTTTTGAGTGATGGGGTGTTAAGCTTTGGACTTCAACATTATGCTTCATCAGAGTTTGAGTTATTGGCTGAAGAATTGTTGATGAGCGACTCTGTAATCAAAGTGTATGGGGCCTTACGCATGACCGTAAAAATGTTCTTGATGTGGAATTCCAAGATGCTCATTGATGGTGGAGGGGAAGAAGCTGTTGAGACATCCTTACTCGAGTCTAGCAACTTGGTGGTTCTCAGGCGATCATCTGTGATACACTCTAATGCAAATCTGGGAGTTCATGGACAAGGTTTATTGAACTTATCAGGACCTGGAGATTGGATTCAAGCACAGCGTCTGGTTTTATCGCTATTCTACAGTATTCATGTTGGACCGGGATCTGTTTTGCGTGGTCCTTTAGAGAATGCCACATCTGATTCTGTAACACCAAAGCTTTATTGTGAAAACAAACATTGCCCCTATGAGCTACTTCATCCACCTGAAGATTGCAATGTGAACTCATCCCTGTCCTTTACTCTTCAGGTATGTCGAGTTGAGGATATCATTATTGAAGGCCTCATAAAAGGATCTGTTGTTAATTTTCACCGGGCAAGGACCATTGCTATCCATTCTTCTGGAGAAATAAGTACATCAGGAATGGGTTGTACTGGTGGTATTGGCAGTGGCAACATTTTAAAAAATGGAATTGGTAGTGGTGGCGGGCATGGAGGTAAAGGTGGGGTGGCATGTTATAATGGCAGTTGTGTTGAGGGTGGAATCTCATATGGGAATGCAAAGTTGCCTTGTGAACTTGGTAGTGGAAGTGGATATGACCCTTCAGCTGGCTTAACTGCTGGCGGTGGTATTATCATAATGGGTTCTTCAGAACATCCCTTGTCAAGTTTGTCTGTTGAAGGTGCGATGACAGCTGATGGTGAAAGTTTTGAAGGGACTGCTGTGGAGGAAAATTTTGCCCTTGTTGATAACACAACTGGAGGGCCTGGGGGTGGGTCTGGTGGAACTATACTTCTGTTCTTGCGAACACTAGCTCTTGGTGAGACTGCCATTCTTTCAAGTGTTGGGGGATATGGTAGTTCCATCGGCAGTGGTGGAGGCGGTGGTGGAAGGATTCATTTTCATTGGTCGGATATTCCCACTGGAGATGTGTATCAGCCCATTGCAAGTGTGGACGGAAGCATCCTTGCAGGGGGAGGGGTGGGTGGAGACCAGGGTGGTGCTGGAGAAAATGGAACATTAACGGGTACAGATTGCCCAAAAGGTCTCTATGGGACCTTTTGTGAGGCATGTCCAGCTGGCACTTATAAGAATGCTATTGGGTCTGATATGGCACTTTGTCATCATTGTCCTGCTACTCAGCTTCCCGCTCGTGCGATTTATATTTCTGTCCGAGGAGGTGTTGCTGAGATTCCTTGTCCTTACAAATGCATTTCAGACAGATATCACATGCCAAACTGTTTTACAGCCCTTGAAGAACTGATTTACACATTTGGTGGGCCTTGGCTATTCGGTCTTCTTCTGATCGGGCTCCTCATCTTGTTGGCTTTGGTACTCAGTGTTGCGCGTATGAAATTGGTTGGGGTTGATGAATTACCAGGCCCTGCTCCCACTCAACATGGCTCACAAATAGACCACTCTTTCCCTTTCCTAGAGTCATTGAATGAGGTTTTAGAAACAAACAGGGCTGAGGAGTCACAGAGCCATGTGCACCGGATGTACTTTATGGGTCCTAATACTTTTGGTGATCCTTGGCATTTGCCCCACACACCTCCAGAACAAATAAAAGAGATTGTATATGAGGGGCCATTCAATACATTTGTCGATGAGATTAATTCCATAGCCACTTATCAATGGTGGGAGGGAGCAATGTACATCATTCTTTCTGTTCTTGCATACCCCCTTGCATGGTCATGGCAGCAGTGGCGCCGAAGATTGAAATTGCAACATCTGCGTGAATTTGTTCGATCGGAGTATGATCATGCCTGCTTACGGTCTTGCCGTTCGCGTGCTTTATATGAAGGGATTAAGGTAGCTGCAACTTCTGATTTAATGCTAGCATATATGGACTTCTTTCTTGGTGGTGATGAAAAGAGAACTGATCTTCCCCCTCGTTTACATCAAAGATTTCCTATTTCATTACCTTTTGGAGGTGATGGAAGTTACATGGCCCCTTTCTCACTTCACAGTGATAACATTCTTACAAGCCTCATGAGTCAGTCAGTCCCGCCTACCGCATGGTACCGTATGGTTGCTGGTTTGAATGCACAGTTACGCTTAGTTTGCCGTGGGCGGCTAAGAGTTACACTTCAGCCTGTCCTTCAGTGGCTAGAACATTATGCCAATCCTGCTTTGAAGATGTATGGTGTACGTGTTGATCTTGCCTGGTTTCAGGCTACATCTTGTGGTTATTGTCATTATGGGCTCGTGGTGGATGTTCTTGAAGAAGACAGTGAACCTGCCTCTGTTAGGAGCATTGATGGAGCCATACGAACTGAAGAATCACGTGCAAACTACAGAGAAGATTCATCTGGTCATTTGAGAGAATCGCTCCTAAACCAGTCTCTCAGAAGTGAAAATTTTATGAGGCCAAAAAGAGAATACGGAGGGATTATAGATGCTAACAACTTACAAACGCTTAAAGAACAGAGAGACATGTTTTATCTTCTCTCTTTTATACTTCATAATACTAAACCTGTTGGACACCAGGATCTCGTTGGTTTAGTCATTTCAATGCTTCTTTTAGGAGATTTGAGCTTAGTTTTGCTTACATTACTCCAGCTGTATTCGATTTCATTGGTGGACGTCTTTCTGGTTCTATTTATTTTACCCCTTGGCATACTTCTCCCGTTTCCTGCTGGTATCAATGCTCTATTCAGTCATGGACCTAGACGTTCTGCTGGGCTTGCACGTCTATATGCTTTGTGGAACCTTACATCCTTGTTTAATGTTGCGGTTGCTTTTGGTTGCGGTTATGTTCATTATTTGACTCAATCATCAAGTAAAAAACATGCGTTTCAACCCTGGAGTAACATGGACGAAAGTGAATGGTGGATTTTTCCCGCTGGTCTGCTGCTTTGTAAAGTTTTTCAATCCCAGCTGATCAATTGGCACGTTGCGAATCTGGAGATTCAAGACCGTTCGTTGTACAGCAATGATTTTGAGCTCTTCTGGCATTCGTGA
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
No pathways identified.
Pfam Domains
Protein Families
No domains found.

Protein Analysis

1450

Amino Acids

155.35

Weight (kDa)

5.59

Isoelectric Point (pI)

48.72

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
DUF8003 PF26010 804 - 877 2e-34 Domain of unknown function (DUF8003)
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

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Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AanI TTATAA 2 cut(s) 1905, 2475
AatII GACGTC 1 cut(s) 3975
Acc36I ACCTGC 1 cut(s) 3634
Acc65I GGTACC 1 cut(s) 3408
AccB1I GGYRCC 2 cut(s) 3093, 3408
AccBSI CCGCTC 2 cut(s) 190, 2537
AccI GTMKAC 2 cut(s) 632, 674
AccII CGCG 6 cut(s) 89, 344, 359, 404, 2734, 3175
AccIII TCCGGA 1 cut(s) 595
AclWI GGATC 5 cut(s) 811, 1558, 1747, 2909, 3885
AcoI YGGCCR 1 cut(s) 414
AcsI RAATTY 5 cut(s) 942, 1330, 2119, 3125, 3745
AcuI CTGAAG 9 cut(s) 789, 1272, 1674, 1676, 1974, 2019, 3461, 3473, 3684
AcvI CACGTG 2 cut(s) 249, 3674
AcyI GRCGYC 4 cut(s) 228, 243, 3094, 3972
AdeI CACNNNGTG 1 cut(s) 438
AflIII ACRYGT 6 cut(s) 248, 285, 402, 603, 3538, 3822
AjiI CACGTC 3 cut(s) 286, 604, 4079
AjnI CCWGG 8 cut(s) 1161, 1483, 2152, 2379, 2764, 3551, 3873, 4190
AloI GAACNNNNNNTCC 4 cut(s) 3256, 3288, 4088, 4120
Alw21I GWGCWC 3 cut(s) 432, 2880, 4335
Alw26I GTCTC 8 cut(s) 208, 265, 1367, 2204, 2370, 2438, 3735, 3813
Alw44I GTGCAC 1 cut(s) 2876
AlwI GGATC 5 cut(s) 811, 1558, 1747, 2909, 3885
AlwNI CAGNNNCTG 3 cut(s) 1484, 1510, 2771
Ama87I CYCGRG 2 cut(s) 323, 1385
Aor13HI TCCGGA 1 cut(s) 595
ApaLI GTGCAC 1 cut(s) 2876
ApeKI GCWGC 7 cut(s) 339, 354, 396, 637, 3085, 3203, 4240
ApoI RAATTY 5 cut(s) 942, 1330, 2119, 3125, 3745
AseI ATTAAT 1 cut(s) 3000
Asp700I GAANNNNTTC 2 cut(s) 1068, 3129
Asp718I GGTACC 1 cut(s) 3408
AspLEI GCGC 5 cut(s) 344, 359, 469, 2734, 3096
AsuC2I CCSGG 5 cut(s) 324, 325, 418, 1548, 1760
AsuNHI GCTAGC 1 cut(s) 3223
AvaI CYCGRG 2 cut(s) 323, 1385
AvaII GGWCC 7 cut(s) 1481, 1544, 1565, 1767, 2442, 2897, 4052
BaeGI GKGCMC 1 cut(s) 2880
BanI GGYRCC 2 cut(s) 3093, 3408
BanII GRGCYC 4 cut(s) 432, 2700, 3594, 4335
BauI CACGAG 3 cut(s) 625, 2538, 3593
BbrPI CACGTG 2 cut(s) 249, 3674
BbsI GAAGAC 2 cut(s) 2675, 3621
Bbv12I GWGCWC 3 cut(s) 432, 2880, 4335
BbvCI CCTCAGC 1 cut(s) 2856
BbvI GCAGC 7 cut(s) 326, 341, 408, 624, 3097, 3190, 4227
BccI CCATC 8 cut(s) 527, 869, 1187, 1343, 2078, 2258, 3317, 3641
BceAI ACGGC 6 cut(s) 181, 450, 475, 495, 3153, 3438
BciT130I CCWGG 8 cut(s) 1163, 1485, 2154, 2381, 2766, 3553, 3875, 4192
BclI TGATCA 2 cut(s) 3145, 4269
BcnI CCSGG 5 cut(s) 324, 325, 418, 1548, 1760
BcoDI GTCTC 8 cut(s) 208, 265, 1367, 2204, 2370, 2438, 3735, 3813
BfaI CTAG 8 cut(s) 72, 651, 1392, 2198, 2819, 3224, 3497, 4056
BfmI CTRYAG 4 cut(s) 638, 919, 1528, 3682
BfoI RGCGCY 2 cut(s) 470, 3097
BfuAI ACCTGC 1 cut(s) 3634
Bme18I GGWCC 7 cut(s) 1481, 1544, 1565, 1767, 2442, 2897, 4052
BmeT110I CYCGRG 2 cut(s) 323, 1385
BmgBI CACGTC 3 cut(s) 286, 604, 4079
BmsI GCATC 5 cut(s) 607, 1000, 1329, 2358, 3775
BmtI GCTAGC 1 cut(s) 3227
BpiI GAAGAC 2 cut(s) 2675, 3621
BplI GAGNNNNNCTC 4 cut(s) 502, 534, 3702, 3734
BpmI CTGGAG 9 cut(s) 1506, 1809, 2166, 2331, 2412, 2925, 3929, 4213, 4313
Bpu10I CCTNAGC 2 cut(s) 391, 2856
BpuMI CCSGG 5 cut(s) 324, 325, 418, 1548, 1760
BsaAI YACGTR 3 cut(s) 249, 3539, 3674
BsaBI GATNNNNATC 2 cut(s) 138, 2319
BsaHI GRCGYC 4 cut(s) 228, 243, 3094, 3972
BsaI GGTCTC 3 cut(s) 208, 2370, 2438
BsaWI WCCGGW 2 cut(s) 595, 2880
BsaXI ACNNNNNCTCC 4 cut(s) 590, 620, 702, 732
Bse118I RCCGGY 2 cut(s) 166, 646
Bse1I ACTGG 5 cut(s) 847, 1825, 2149, 2314, 3727
Bse3DI GCAATG 7 cut(s) 448, 1624, 1669, 1770, 2328, 3042, 4327
Bse8I GATNNNNATC 2 cut(s) 138, 2319
BseAI TCCGGA 1 cut(s) 595
BseBI CCWGG 8 cut(s) 1163, 1485, 2154, 2381, 2766, 3553, 3875, 4192
BseGI GGATG 9 cut(s) 622, 1376, 1645, 1673, 2041, 2349, 2889, 3607, 4103
BseJI GATNNNNATC 2 cut(s) 138, 2319
BseMI GCAATG 7 cut(s) 448, 1624, 1669, 1770, 2328, 3042, 4327
BseMII CTCAG 6 cut(s) 1423, 2540, 2565, 2737, 2847, 3748
BseNI ACTGG 5 cut(s) 847, 1825, 2149, 2314, 3727
BseRI GAGGAG 8 cut(s) 17, 20, 71, 74, 171, 2577, 2690, 2873
BseSI GKGCMC 1 cut(s) 2880
BseXI GCAGC 7 cut(s) 326, 341, 408, 624, 3097, 3190, 4227
BseYI CCCAGC 4 cut(s) 488, 995, 4067, 4263
Bsh1236I CGCG 6 cut(s) 89, 344, 359, 404, 2734, 3175
Bsh1285I CGRYCG 1 cut(s) 3137
BshNI GGYRCC 2 cut(s) 3093, 3408
BsiEI CGRYCG 1 cut(s) 3137
BsiHKAI GWGCWC 3 cut(s) 432, 2880, 4335
BsiHKCI CYCGRG 2 cut(s) 323, 1385
BsiSI CCGG 8 cut(s) 167, 324, 417, 596, 647, 1547, 1759, 2881
BslFI GGGAC 5 cut(s) 1012, 1124, 2116, 2455, 3377
BsmAI GTCTC 8 cut(s) 208, 265, 1367, 2204, 2370, 2438, 3735, 3813
BsmFI GGGAC 5 cut(s) 1012, 1124, 2116, 2455, 3377
BsmI GAATGC 6 cut(s) 35, 1582, 1948, 2485, 3436, 4342
Bso31I GGTCTC 3 cut(s) 208, 2370, 2438
BsoBI CYCGRG 2 cut(s) 323, 1385
Bsp1286I GDGCHC 5 cut(s) 432, 2700, 2880, 3594, 4335
Bsp13I TCCGGA 1 cut(s) 595
Bsp1407I TGTACA 3 cut(s) 288, 3039, 4314
BspCNI CTCAG 6 cut(s) 1422, 2539, 2566, 2736, 2848, 3747
BspEI TCCGGA 1 cut(s) 595
BspFNI CGCG 6 cut(s) 89, 344, 359, 404, 2734, 3175
BspHI TCATGA 1 cut(s) 3378
BspMAI CTGCAG 1 cut(s) 642
BspMI ACCTGC 1 cut(s) 3634
BspOI GCTAGC 1 cut(s) 3227
BspPI GGATC 5 cut(s) 811, 1558, 1747, 2909, 3885
BspQI GCTCTTC 1 cut(s) 4340
BspT107I GGYRCC 2 cut(s) 3093, 3408
BspTNI GGTCTC 3 cut(s) 208, 2370, 2438
BsrBI CCGCTC 2 cut(s) 190, 2537
BsrDI GCAATG 7 cut(s) 448, 1624, 1669, 1770, 2328, 3042, 4327
BsrFI RCCGGY 2 cut(s) 166, 646
BsrGI TGTACA 3 cut(s) 288, 3039, 4314
BsrI ACTGG 5 cut(s) 847, 1825, 2149, 2314, 3727
BssAI RCCGGY 2 cut(s) 166, 646
BssNI GRCGYC 4 cut(s) 228, 243, 3094, 3972
BssSI CACGAG 3 cut(s) 625, 2538, 3593
BssT1I CCWWGG 3 cut(s) 2669, 2918, 4000
Bst2BI CACGAG 3 cut(s) 625, 2538, 3593
Bst2UI CCWGG 8 cut(s) 1163, 1485, 2154, 2381, 2766, 3553, 3875, 4192
Bst6I CTCTTC 4 cut(s) 149, 895, 1695, 4340
BstACI GRCGYC 4 cut(s) 228, 243, 3094, 3972
BstAPI GCANNNNNTGC 1 cut(s) 3118
BstAUI TGTACA 3 cut(s) 288, 3039, 4314
BstBAI YACGTR 3 cut(s) 249, 3539, 3674
BstDSI CCRYGG 1 cut(s) 3454
BstENI CCTNNNNNAGG 2 cut(s) 3315, 3634
BstF5I GGATG 9 cut(s) 622, 1376, 1645, 1673, 2041, 2349, 2889, 3607, 4103
BstFNI CGCG 6 cut(s) 89, 344, 359, 404, 2734, 3175
BstH2I RGCGCY 2 cut(s) 470, 3097
BstHHI GCGC 5 cut(s) 344, 359, 469, 2734, 3096
BstMAI GTCTC 8 cut(s) 208, 265, 1367, 2204, 2370, 2438, 3735, 3813
BstMCI CGRYCG 1 cut(s) 3137
BstNI CCWGG 8 cut(s) 1163, 1485, 2154, 2381, 2766, 3553, 3875, 4192
BstNSI RCATGY 5 cut(s) 1902, 2460, 2620, 3826, 4181
BstSFI CTRYAG 4 cut(s) 638, 919, 1528, 3682
BstSLI GKGCMC 1 cut(s) 2880
BstUI CGCG 6 cut(s) 89, 344, 359, 404, 2734, 3175
BstV1I GCAGC 7 cut(s) 326, 341, 408, 624, 3097, 3190, 4227
BstV2I GAAGAC 2 cut(s) 2675, 3621
BstX2I RGATCY 4 cut(s) 803, 1550, 1739, 3877
BstXI CCANNNNNNTGG 2 cut(s) 1149, 1787
BstYI RGATCY 4 cut(s) 803, 1550, 1739, 3877
BtgI CCRYGG 1 cut(s) 3454
BtgZI GCGATG 1 cut(s) 2087
BtrI CACGTC 3 cut(s) 286, 604, 4079
BtsCI GGATG 9 cut(s) 622, 1376, 1645, 1673, 2041, 2349, 2889, 3607, 4103
BtsI GCAGTG 3 cut(s) 1840, 2263, 3095
BtsIMutI CAGTG 8 cut(s) 1840, 2263, 2307, 2731, 3095, 3361, 3497, 3625
BveI ACCTGC 1 cut(s) 3634
CaiI CAGNNNCTG 3 cut(s) 1484, 1510, 2771
CciI TCATGA 1 cut(s) 3378
CfoI GCGC 5 cut(s) 344, 359, 469, 2734, 3096
Cfr10I RCCGGY 2 cut(s) 166, 646
Cfr9I CCCGGG 1 cut(s) 323
CseI GACGC 6 cut(s) 78, 217, 251, 393, 643, 1496
DinI GGCGCC 1 cut(s) 3095
DraI TTTAAA 1 cut(s) 1848
DraIII CACNNNGTG 1 cut(s) 438
EaeI YGGCCR 1 cut(s) 414
Eam1104I CTCTTC 4 cut(s) 149, 895, 1695, 4340
EarI CTCTTC 4 cut(s) 149, 895, 1695, 4340
EciI GGCGGA 4 cut(s) 34, 162, 174, 795
Ecl136II GAGCTC 2 cut(s) 430, 4333
Eco130I CCWWGG 3 cut(s) 2669, 2918, 4000
Eco147I AGGCCT 1 cut(s) 1728
Eco24I GRGCYC 4 cut(s) 432, 2700, 3594, 4335
Eco31I GGTCTC 3 cut(s) 208, 2370, 2438
Eco32I GATATC 2 cut(s) 1714, 2612
Eco47I GGWCC 7 cut(s) 1481, 1544, 1565, 1767, 2442, 2897, 4052
Eco53kI GAGCTC 2 cut(s) 430, 4333
Eco57I CTGAAG 9 cut(s) 789, 1272, 1674, 1676, 1974, 2019, 3461, 3473, 3684
Eco72I CACGTG 2 cut(s) 249, 3674
Eco88I CYCGRG 2 cut(s) 323, 1385
EcoICRI GAGCTC 2 cut(s) 430, 4333
EcoNI CCTNNNNNAGG 2 cut(s) 3315, 3634
EcoO109I RGGNCCY 6 cut(s) 1293, 1481, 2150, 2442, 2768, 2897
EcoRI GAATTC 1 cut(s) 1330
EcoRII CCWGG 8 cut(s) 1161, 1483, 2152, 2379, 2764, 3551, 3873, 4190
EcoRV GATATC 2 cut(s) 1714, 2612
EcoT14I CCWWGG 3 cut(s) 2669, 2918, 4000
EcoT22I ATGCAT 2 cut(s) 622, 2600
EcoT38I GRGCYC 4 cut(s) 432, 2700, 3594, 4335
EgeI GGCGCC 1 cut(s) 3095
EheI GGCGCC 1 cut(s) 3095
ErhI CCWWGG 3 cut(s) 2669, 2918, 4000
FalI AAGNNNNNCTT 2 cut(s) 293, 325
FaqI GGGAC 5 cut(s) 1012, 1124, 2116, 2455, 3377
FauI CCCGC 9 cut(s) 201, 532, 552, 662, 983, 1865, 2542, 3402, 4237
FauNDI CATATG 1 cut(s) 1936
FbaI TGATCA 2 cut(s) 3145, 4269
FblI GTMKAC 2 cut(s) 632, 674
FokI GGATG 9 cut(s) 629, 1363, 1632, 1660, 2028, 2336, 2896, 3614, 4090
FriOI GRGCYC 4 cut(s) 432, 2700, 3594, 4335
FspBI CTAG 8 cut(s) 72, 651, 1392, 2198, 2819, 3224, 3497, 4056
GlaI GCGC 5 cut(s) 343, 358, 468, 2733, 3095
GsaI CCCAGC 4 cut(s) 492, 999, 4071, 4267
GsuI CTGGAG 9 cut(s) 1506, 1809, 2166, 2331, 2412, 2925, 3929, 4213, 4313
HaeII RGCGCY 2 cut(s) 470, 3097
HapII CCGG 8 cut(s) 167, 324, 417, 596, 647, 1547, 1759, 2881
HgaI GACGC 6 cut(s) 78, 217, 251, 393, 643, 1496
HhaI GCGC 5 cut(s) 344, 359, 469, 2734, 3096
Hin1I GRCGYC 4 cut(s) 228, 243, 3094, 3972
Hin6I GCGC 5 cut(s) 342, 357, 467, 2732, 3094
HinP1I GCGC 5 cut(s) 342, 357, 467, 2732, 3094
HincII GTYRAC 3 cut(s) 633, 675, 1053
HindII GTYRAC 3 cut(s) 633, 675, 1053
HindIII AAGCTT 3 cut(s) 590, 1204, 1604
HpaII CCGG 8 cut(s) 167, 324, 417, 596, 647, 1547, 1759, 2881
Hpy99I CGWCG 7 cut(s) 202, 233, 287, 409, 481, 637, 679
HpyCH4IV ACGT 9 cut(s) 248, 285, 603, 3538, 3673, 3972, 4060, 4078, 4281
HpySE526I ACGT 9 cut(s) 248, 285, 603, 3538, 3673, 3972, 4060, 4078, 4281
Hsp92I GRCGYC 4 cut(s) 228, 243, 3094, 3972
HspAI GCGC 5 cut(s) 342, 357, 467, 2732, 3094
KasI GGCGCC 1 cut(s) 3093
Kpn2I TCCGGA 1 cut(s) 595
KpnI GGTACC 1 cut(s) 3412
KroI GCCGGC 2 cut(s) 166, 646
KroNI GCCGGC 2 cut(s) 168, 648
Ksp22I TGATCA 2 cut(s) 3145, 4269
LguI GCTCTTC 1 cut(s) 4340
Lsp1109I GCAGC 7 cut(s) 326, 341, 408, 624, 3097, 3190, 4227
LweI GCATC 5 cut(s) 607, 1000, 1329, 2358, 3775
MaeI CTAG 8 cut(s) 72, 651, 1392, 2198, 2819, 3224, 3497, 4056
MaeII ACGT 9 cut(s) 248, 285, 603, 3538, 3673, 3972, 4060, 4078, 4281
MaeIII GTNAC 8 cut(s) 380, 878, 1594, 2862, 3329, 3438, 3469, 4196
MbiI CCGCTC 2 cut(s) 190, 2537
MfeI CAATTG 1 cut(s) 4273
MflI RGATCY 4 cut(s) 803, 1550, 1739, 3877
MhlI GDGCHC 5 cut(s) 432, 2700, 2880, 3594, 4335
MluI ACGCGT 1 cut(s) 402
Mly113I GGCGCC 1 cut(s) 3094
MlyI GAGTC 7 cut(s) 1162, 1265, 1397, 2831, 2870, 3391, 4150
MmeI TCCRAC 3 cut(s) 1522, 2277, 3847
Mph1103I ATGCAT 2 cut(s) 622, 2600
MroI TCCGGA 1 cut(s) 595
MroNI GCCGGC 2 cut(s) 166, 646
MroXI GAANNNNTTC 2 cut(s) 1068, 3129
MslI CAYNNNNRTG 5 cut(s) 625, 1434, 3079, 3120, 3899
MspA1I CMGCKG 6 cut(s) 1994, 2081, 2465, 3949, 4232, 4267
MspI CCGG 8 cut(s) 167, 324, 417, 596, 647, 1547, 1759, 2881
MunI CAATTG 1 cut(s) 4273
Mva1269I GAATGC 6 cut(s) 35, 1582, 1948, 2485, 3436, 4342
MvaI CCWGG 8 cut(s) 1163, 1485, 2154, 2381, 2766, 3553, 3875, 4192
MvnI CGCG 6 cut(s) 89, 344, 359, 404, 2734, 3175
NaeI GCCGGC 2 cut(s) 168, 648
NarI GGCGCC 1 cut(s) 3094
NciI CCSGG 5 cut(s) 324, 325, 418, 1548, 1760
NdeI CATATG 1 cut(s) 1936
NgoMIV GCCGGC 2 cut(s) 166, 646
NheI GCTAGC 1 cut(s) 3223
NmeAIII GCCGAG 3 cut(s) 201, 443, 689
NmuCI GTSAC 1 cut(s) 2862
NsiI ATGCAT 2 cut(s) 622, 2600
NspI RCATGY 5 cut(s) 1902, 2460, 2620, 3826, 4181
PaeR7I CTCGAG 1 cut(s) 1385
PagI TCATGA 1 cut(s) 3378
PceI AGGCCT 1 cut(s) 1728
PciI ACATGT 1 cut(s) 3822
PciSI GCTCTTC 1 cut(s) 4340
PcsI WCGNNNNNNNCGW 1 cut(s) 3166
PctI GAATGC 6 cut(s) 35, 1582, 1948, 2485, 3436, 4342
PdiI GCCGGC 2 cut(s) 168, 648
PdmI GAANNNNTTC 2 cut(s) 1068, 3129
Ple19I CGATCG 1 cut(s) 3137
PleI GAGTC 7 cut(s) 1161, 1265, 1396, 2830, 2869, 3390, 4150
PluTI GGCGCC 1 cut(s) 3097
PmaCI CACGTG 2 cut(s) 249, 3674
PmlI CACGTG 2 cut(s) 249, 3674
PpsI GAGTC 7 cut(s) 1161, 1265, 1396, 2830, 2869, 3390, 4150
Ppu21I YACGTR 3 cut(s) 249, 3539, 3674
PpuMI RGGWCCY 3 cut(s) 1481, 2442, 2897
PscI ACATGT 1 cut(s) 3822
PshBI ATTAAT 1 cut(s) 3000
PsiI TTATAA 2 cut(s) 1905, 2475
Psp124BI GAGCTC 2 cut(s) 432, 4335
Psp5II RGGWCCY 3 cut(s) 1481, 2442, 2897
Psp6I CCWGG 8 cut(s) 1161, 1483, 2152, 2379, 2764, 3551, 3873, 4190
PspCI CACGTG 2 cut(s) 249, 3674
PspFI CCCAGC 4 cut(s) 488, 995, 4067, 4263
PspGI CCWGG 8 cut(s) 1161, 1483, 2152, 2379, 2764, 3551, 3873, 4190
PspPPI RGGWCCY 3 cut(s) 1481, 2442, 2897
PspXI VCTCGAGB 1 cut(s) 1385
PstI CTGCAG 1 cut(s) 642
PstNI CAGNNNCTG 3 cut(s) 1484, 1510, 2771
PsuI RGATCY 4 cut(s) 803, 1550, 1739, 3877
PvuI CGATCG 1 cut(s) 3137
PvuII CAGCTG 5 cut(s) 1994, 2081, 2465, 3949, 4267
RseI CAYNNNNRTG 5 cut(s) 625, 1434, 3079, 3120, 3899
SacI GAGCTC 2 cut(s) 432, 4335
SalI GTCGAC 2 cut(s) 631, 673
SapI GCTCTTC 1 cut(s) 4340
SchI GAGTC 7 cut(s) 1162, 1265, 1397, 2831, 2870, 3391, 4150
SduI GDGCHC 5 cut(s) 432, 2700, 2880, 3594, 4335
SfaNI GCATC 5 cut(s) 607, 1000, 1329, 2358, 3775
SfcI CTRYAG 4 cut(s) 638, 919, 1528, 3682
SfoI GGCGCC 1 cut(s) 3095
Sfr274I CTCGAG 1 cut(s) 1385
SinI GGWCC 7 cut(s) 1481, 1544, 1565, 1767, 2442, 2897, 4052
SlaI CTCGAG 1 cut(s) 1385
SmaI CCCGGG 1 cut(s) 325
SmiMI CAYNNNNRTG 5 cut(s) 625, 1434, 3079, 3120, 3899
SmlI CTYRAG 1 cut(s) 1385
SmoI CTYRAG 1 cut(s) 1385
SseBI AGGCCT 1 cut(s) 1728
SspDI GGCGCC 1 cut(s) 3093
SspMI CTAG 8 cut(s) 72, 651, 1392, 2198, 2819, 3224, 3497, 4056
SstI GAGCTC 2 cut(s) 432, 4335
StuI AGGCCT 1 cut(s) 1728
StyI CCWWGG 3 cut(s) 2669, 2918, 4000
TaiI ACGT 9 cut(s) 251, 288, 606, 3541, 3676, 3975, 4063, 4081, 4284
TaqI TCGA 8 cut(s) 632, 674, 1021, 1386, 1702, 2991, 3133, 3956
TaqII GACCGA 2 cut(s) 190, 2669
TatI WGTACW 6 cut(s) 288, 1799, 1817, 2886, 3039, 4314
TauI GCSGC 7 cut(s) 166, 190, 228, 499, 502, 505, 3463
TscAI CASTG 8 cut(s) 1840, 2263, 2314, 2731, 3095, 3361, 3497, 3625
TseFI GTSAC 1 cut(s) 2862
TseI GCWGC 7 cut(s) 339, 354, 396, 637, 3085, 3203, 4240
Tsp45I GTSAC 1 cut(s) 2862
TspGWI ACGGA 3 cut(s) 567, 2358, 3786
TspMI CCCGGG 1 cut(s) 323
TspRI CASTG 8 cut(s) 1840, 2263, 2314, 2731, 3095, 3361, 3497, 3625
VneI GTGCAC 1 cut(s) 2876
VpaK11BI GGWCC 7 cut(s) 1481, 1544, 1565, 1767, 2442, 2897, 4052
VspI ATTAAT 1 cut(s) 3000
XagI CCTNNNNNAGG 2 cut(s) 3315, 3634
XapI RAATTY 5 cut(s) 942, 1330, 2119, 3125, 3745
XceI RCATGY 5 cut(s) 1902, 2460, 2620, 3826, 4181
XcmI CCANNNNNNNNNTGG 3 cut(s) 2335, 2387, 3881
XhoI CTCGAG 1 cut(s) 1385
XmaI CCCGGG 1 cut(s) 323
XmiI GTMKAC 2 cut(s) 632, 674
XmnI GAANNNNTTC 2 cut(s) 1068, 3129
XspI CTAG 8 cut(s) 72, 651, 1392, 2198, 2819, 3224, 3497, 4056
ZraI GACGTC 1 cut(s) 3973
Zsp2I ATGCAT 2 cut(s) 622, 2600
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.