pycom08g17580

Pyruvate phosphate dikinase, PEP/pyruvate binding domain

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
pyrus_communis
Chr8
OG0004913
Physical Location & Seq
Reverse (-)
17799015 .. 17812138
13124 bp
Loading structure...
UTR
Exon/CDS
Intron
N/A

Sequence Viewer

Length: 3873 bp
ATGGCCGCCGCCGGTAACCACCCTTCCGGCTCATCACAGGCTCCTGCTCCTCCTCCTCCTCGTCTCCACCAGTTTGATCTCATTGACCGCATGCAACTTCAGATTAATGTCTCGGGTTCTTCGAAAGGACGCAATGCAAGACTTGAATTTCAGCTCAGCAACTGCCCCAGCACTTGGGTTCTTCACTGGGGTTGCATTCTCCGTGGAAACAAGAACTGGTTCGTTCCCGCTGATCGCTCTTCGGGTTCACAAGCTTACAAGCAAGGCGCATTGCAGACACAATTCACTAAGAAAGGAGAATTGTATTTGTTAACCATCGAGTTGCGGGACCCCAACGTACATGCTATTGAATTTGTACTTAAAGATGGCAGTCGCGAGAGATGGTTGAAAATGAATCACGGGAACTTCCGTGTAGAAATCCCTGAGACCGATCCAACTACTTTAAGTCCCCCAATACCAAAAGAGCTGATTGAACAGAAAAGCAAGGGCAGGCCCATGAGCTCGCCACAACAAGAAAAGGAAGATTACAAGGATACTTTAATAGGGCTACAAAGTCAGATATCAAAGGGAATATCCTTTAATGAGTTGCAGCATAGTTTTTCAACCTCCAGTCCTAGAAGAATGGTTAATAATGGAGAGAAACTGAGGAGTGGGATGCCATACTCGTACAAAAGGAAACATAATGTTGAGCAGTGGTTGCAAAAGCATTCAACAGCACCTGCCAAAAACGCTAGTATGCCAAATTCAGTTCTCGTGGATCTTGTAGAAAAATCTATGGGAGGAGACAATGTGGTCTCACGGATAACTTATCAAATTGGCAACTATGACATAGTGGTCCTTTCAAAAATGGATAGGGGTGACTTTCACATATTGGTTGCTACAAATATGAGAGGTGATACAGTTCTTCACTGGGGTGTTTCCAAATGTTCCCCCGGGGATTGGTTGGCTCCTCCACCAGAGATATTGCCTAAAAAATCAAATTTAGTTCCTGGAGCATGTCAGACTTATTTTACAGAAATATCAACTGGAAAGGGTTCCTTTCAGATTGTAGATATTAATCTTCAGCAAAGCAATTTGGTGGGTATCCAATTTGTGATATGGTCTGGTAGGTCTTCTTGGATAAAAAACAACGGAGCAAACTTCTTTGTAGGCGTGAGTCGTGTGACACCAGTGACTTCTACTGCAAAGGCTAGTGGGAATGGCGATGGAATCTGCAAATGGTTACTTGATGAAATATCTCGAAGAGAGAAGGAGGCCGAGAGATCGTTGATGCACAGATTCAACATTGCAACGGAACTCACAGAACGCTGCAAAAGTGAAGGGGAGTTCGGGCTTGTTGGTATATTGGTATGGTTGAGGTTCATGTCATGCAGGCATCTAACATGGAACAAGAACTACAATGTGAAACCTCGTGAAATTAGTGAAGCTCAGGATAGGTTTACCAATCTGCTACAAAGAATATATTTAAACCAGCCTAATGATCGGGAAATTGTGAGATTACTAATGACACATGTTGGTCGTGGAGGTCAGGGAGATGTTGGACAGAGAATTAGAGATGAAATACTTGTGATTCAGAGAAATAATGACTGTAAGGGTGGCATGATGGAGGAATGGCACCAAAAGTTGCACAATAATAGTAGCCCAGATGATGTGATAATTTGTGAGGCGCTCTTAAATTTCATCAAGTCTGGTTTTAGAGTTGATGTTTATTGGAAAACATTAAATGCCAATGGTCTCACAAAAGATAAACTTGCAAGTTATGACCGCCCAATTGTGTCAGAGCCGCATTTCAGGGCTGATACTAAGGAGGGACTCATCCGTGACCTCACAGCATACTTGAAGACATTAAAGGCTGTTCATTCAGGTGCTGACCTTGAATCAGCTATTGAAGTTTTGGTTCCCTCAAATGAGAGTCGTAATTTCTCAAGCATGGTTGGAGTTAATTATGTTTGTGATTTGTCACCTAGGTTGCAGGAGTGCCTGAACTTTGTCAAAGCACATGTTGGTGATGAAGACATTGTTCCACTAATGGAGAAGTTACTAGAATCTCGAATTGAGCTACGCCCTGCTCTAATCACCAACCACAGAAGATTGAAAGATATACTCTTCCTTGATATTGCTTTGGATTCAGCTGTCAGAACAACCATGGAGAGGGGACTGAAAAATCTGAACTCTGGACACCCACCAGAGATTATGTTCTTCATTTCCTTGGTGCTCGAAAATTTATGTCTGTCAACAGTTGACAATGAAGACCTCATTTACTGTACCAAGGACTGGTACCGTACCTGCGAATTATACAAACCCAATGATGGTCAGTGGGCTTTACAAACAAAGGCCATTCTTGATAGATTACAATTAGTACTTGCTGAGAGGTCTCAGTGTTACCAGAAGAAGATCCAGCCCAGTGCAAAATATCTCGGCAATTTGCTTGGTGTTCAGAAATCGCCGATCGATACTTTCTCTGAGGAGTTAATAAGGGCTGGATCAGCAGCTATTCTGTCTACCCTCATTAATCGCTTTAACCCCATTCTTAGAAAAGCTGCAAACTTGGGCAGTTGGCAGGTTATCAGCCCAGTAGAAGTGTCAGGATTCTTACATTCTGTTAGTGAGCTCATTAGTATTCAGAACAAAGTCTATAAAAAGCCAACCGTTTTAATTGCAACTAGAGTAACGGGAGAAGAGGAGATTCCAGATGGAGTTGTTGCTGTTCTGACACCTGACTTACCAGATGTTTTATCTCATGTCTCTATAAGAGCAAGAAATGACAAGGCATATAACCTTTCATCTTTATTTATCAATTCCCAAATAACCAAACTTGACATCAGTAGCTCCAATCTTTCTTACAAGTCATTTGCTACTCAATCCTCCAATCATCCAGGATTAACGCTAAAAAAGAAGGCCTTCTGTGGTAAATATGCTGTCTCAATTCAGGAGTTCACTAGTGAGCTGGTTGGTGCAAAATCTTTCAATATAAAAGTTTTGAGAGAAAGGGTGCCGACATGGATTAAAATTCCAATGTCAGTAGCCATACCGTTTGGAGCCTTTGAGAAAGTCCTGCTGGAAGATGTTAATAAGGATATAGCAAATAAGATTTCCTCATTTTACAAATGTGTAAAGAGAGGAGACCTCTCAAAACTTCAAGCAATACGAGACACTGTTCTACAAATGAATGCTCCTATTTCATTGATCCATGAGCTTAAGAGCAAAATGAAAAGTTCAGGAATACCTTGGCCTGGTGATGAAGGTGGCGAGAGGTGGACTCATGCTTGGCAAGCAATAAAGAAGGTTTGGGCTTCAAAGTGGAATGAAAGAGCATTTATTAGTTGTAGGAAAGCTAACTTAGATCATGAAAATATATGCATGGCTGTACTAATTCAAGAAATTATTTGCGCTGATTATGCTTTTGTAATTCACACGAAGAATCCCCTATCAGGGGATACCTCAGAGATATATACAGAGATAGTTAAGGGCTTGGGAGAGACTTTAGTGGGTGCATATCCCGGACGTGCCTTGAGCTTCATTACCAAAAAATCAAATCTAAATTCTCCTGTTGTTACTGGTTATCCAAGCAAGCCAATAGGATTATATAGCAAGCAGTCTATCATATTCAGATCAGACTCAAATGCCGAGGACCTGGAAGGATATGCAGGTGCTGGGCTTTATGACAGTGTGATCATGGATAAGGAGGAGAAGATTGTATTGGATTACTCGAGAGATCGGTTGATTATTGACAGACCCTTCCAAGTCTCAGTCTTGTCGAGAATTGCAGAGGCGGGAAAGATAGTAGAAGGCATTTACGGTCATCCTCAGGACATTGAAGGGGTGGTAAAAGATGAAGTGATCTATGTGGTTCAGTCAAGGCCACAAATCTAG

Protein Analysis

1291

Amino Acids

144.73

Weight (kDa)

8.84

Isoelectric Point (pI)

44.24

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
Ig_N_CWD1 PF23166 23 - 140 9.1e-35 Alpha-glucan water dikinase 1 Ig-like N-terminal domain
DUF7067 PF23229 161 - 201 2.8e-10 Alpha-glucan water dikinase, DUF7067
Ig_N_CWD1 PF23166 281 - 383 3.2e-20 Alpha-glucan water dikinase 1 Ig-like N-terminal domain
GWD1_pHisD PF22973 852 - 924 2.7e-33 Alpha-glucan water dikinase, phosphohistidine-like domain
PPDK_N PF01326 1089 - 1289 2.2e-19 Pyruvate phosphate dikinase, AMP/ATP-binding domain
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

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Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AarI CACCTGC 2 cut(s) 727, 3638
AasI GACNNNNNNGTC 2 cut(s) 791, 833
Acc36I ACCTGC 4 cut(s) 727, 2294, 2551, 3638
Acc65I GGTACC 1 cut(s) 2277
AccB1I GGYRCC 3 cut(s) 1614, 2277, 2992
AccB7I CCANNNNNTGG 2 cut(s) 174, 2274
AccI GTMKAC 1 cut(s) 2501
AccII CGCG 1 cut(s) 375
AciI CCGC 8 cut(s) 6, 9, 88, 228, 325, 1765, 1784, 3773
AclWI GGATC 5 cut(s) 425, 765, 2389, 2491, 3181
AcoI YGGCCR 1 cut(s) 3
AcsI RAATTY 8 cut(s) 146, 350, 742, 979, 1675, 2221, 3009, 3541
AcuI CTGAAG 2 cut(s) 83, 1046
AfaI GTAC 8 cut(s) 339, 357, 668, 2266, 2279, 2284, 2361, 3369
AfiI CCNNNNNNNGG 9 cut(s) 174, 939, 2151, 2274, 2309, 3233, 3431, 3432, 3433
AflII CTTAAG 1 cut(s) 3197
AflIII ACRYGT 2 cut(s) 1510, 1999
AhlI ACTAGT 1 cut(s) 2939
AjiI CACGTC 1 cut(s) 3506
AjnI CCWGG 4 cut(s) 988, 2875, 3232, 3633
AjuI GAANNNNNNNTTGG 2 cut(s) 3683, 3715
AleI CACNNNNGTG 1 cut(s) 912
Alw21I GWGCWC 3 cut(s) 503, 2217, 2613
AlwI GGATC 5 cut(s) 425, 765, 2389, 2491, 3181
AlwNI CAGNNNCTG 4 cut(s) 162, 719, 1868, 3653
Ama87I CYCGRG 3 cut(s) 112, 932, 3709
AoxI GGCC 7 cut(s) 3, 491, 1254, 2334, 2898, 3230, 3860
ApeKI GCWGC 4 cut(s) 589, 1308, 2489, 2540
ApoI RAATTY 8 cut(s) 146, 350, 742, 979, 1675, 2221, 3009, 3541
AseI ATTAAT 3 cut(s) 105, 1056, 2511
Asp700I GAANNNNTTC 3 cut(s) 218, 1033, 2900
Asp718I GGTACC 1 cut(s) 2277
AspA2I CCTAGG 1 cut(s) 1964
AspLEI GCGC 3 cut(s) 269, 1669, 3392
AspS9I GGNCC 4 cut(s) 328, 492, 835, 3631
AsuC2I CCSGG 3 cut(s) 933, 934, 3501
AsuHPI GGTGA 6 cut(s) 869, 905, 1953, 2018, 2068, 3248
AsuII TTCGAA 1 cut(s) 122
AvaI CYCGRG 3 cut(s) 112, 932, 3709
AvaII GGWCC 3 cut(s) 328, 835, 3631
AvrII CCTAGG 1 cut(s) 1964
AxyI CCTNAGG 1 cut(s) 3807
BanI GGYRCC 3 cut(s) 1614, 2277, 2992
BanII GRGCYC 2 cut(s) 503, 2613
BarI GAAGNNNNNNTAC 2 cut(s) 7, 39
BauI CACGAG 2 cut(s) 752, 1410
BbsI GAAGAC 4 cut(s) 1104, 1847, 2019, 2256
Bbv12I GWGCWC 3 cut(s) 503, 2217, 2613
BbvI GCAGC 4 cut(s) 601, 1295, 2501, 2527
BccI CCATC 7 cut(s) 323, 359, 375, 1199, 1597, 2303, 2687
BciT130I CCWGG 4 cut(s) 990, 2877, 3234, 3635
BciVI GTATCC 3 cut(s) 526, 1094, 3430
BclI TGATCA 1 cut(s) 3672
BcnI CCSGG 3 cut(s) 933, 934, 3501
BcuI ACTAGT 1 cut(s) 2939
BfaI CTAG 8 cut(s) 615, 732, 1191, 1965, 2042, 2664, 2940, 3871
BfoI RGCGCY 1 cut(s) 1670
BfrI CTTAAG 1 cut(s) 3197
BfuAI ACCTGC 4 cut(s) 727, 2294, 2551, 3638
BfuI GTATCC 3 cut(s) 526, 1094, 3430
BisI GCNGC 7 cut(s) 6, 9, 590, 1309, 1784, 2490, 2541
BlnI CCTAGG 1 cut(s) 1964
BlpI GCTNAGC 1 cut(s) 155
BlsI GCNGC 7 cut(s) 7, 10, 591, 1310, 1785, 2491, 2542
BmcAI AGTACT 1 cut(s) 2361
Bme1390I CCNGG 7 cut(s) 933, 934, 990, 2877, 3234, 3501, 3635
Bme18I GGWCC 3 cut(s) 328, 835, 3631
BmeT110I CYCGRG 3 cut(s) 112, 932, 3709
BmgBI CACGTC 1 cut(s) 3506
BmgT120I GGNCC 4 cut(s) 328, 492, 835, 3631
BmrFI CCNGG 7 cut(s) 933, 934, 990, 2877, 3234, 3501, 3635
BmrI ACTGGG 4 cut(s) 196, 919, 2397, 2567
BmsI GCATC 3 cut(s) 645, 1260, 1384
BmuI ACTGGG 4 cut(s) 196, 919, 2397, 2567
BpiI GAAGAC 4 cut(s) 1104, 1847, 2019, 2256
BplI GAGNNNNNCTC 4 cut(s) 3111, 3143, 3244, 3276
BpmI CTGGAG 2 cut(s) 592, 1011
Bpu10I CCTNAGC 1 cut(s) 1428
Bpu1102I GCTNAGC 1 cut(s) 155
Bpu14I TTCGAA 1 cut(s) 122
BpuEI CTTGAG 2 cut(s) 1909, 3532
BpuMI CCSGG 3 cut(s) 933, 934, 3501
Bsa29I ATCGAT 1 cut(s) 2451
BsaBI GATNNNNATC 1 cut(s) 1056
BsaI GGTCTC 5 cut(s) 419, 799, 1739, 2379, 3117
BsaXI ACNNNNNCTCC 2 cut(s) 774, 804
Bsc4I CCNNNNNNNGG 9 cut(s) 174, 939, 2151, 2274, 2309, 3233, 3431, 3432, 3433
Bse118I RCCGGY 1 cut(s) 11
Bse21I CCTNAGG 1 cut(s) 3807
Bse3DI GCAATG 3 cut(s) 139, 269, 1284
Bse8I GATNNNNATC 1 cut(s) 1056
BseBI CCWGG 4 cut(s) 990, 2877, 3234, 3635
BseCI ATCGAT 1 cut(s) 2451
BseGI GGATG 4 cut(s) 660, 1815, 2872, 3802
BseJI GATNNNNATC 1 cut(s) 1056
BseLI CCNNNNNNNGG 9 cut(s) 174, 939, 2151, 2274, 2309, 3233, 3431, 3432, 3433
BseMI GCAATG 3 cut(s) 139, 269, 1284
BseXI GCAGC 4 cut(s) 601, 1295, 2501, 2527
BseYI CCCAGC 2 cut(s) 167, 3653
Bsh1236I CGCG 1 cut(s) 375
Bsh1285I CGRYCG 1 cut(s) 2451
BshFI GGCC 7 cut(s) 5, 493, 1256, 2336, 2900, 3232, 3862
BshNI GGYRCC 3 cut(s) 1614, 2277, 2992
BshVI ATCGAT 1 cut(s) 2451
BsiEI CGRYCG 1 cut(s) 2451
BsiHKAI GWGCWC 3 cut(s) 503, 2217, 2613
BsiHKCI CYCGRG 3 cut(s) 112, 932, 3709
BsiSI CCGG 4 cut(s) 12, 27, 933, 3501
BslFI GGGAC 4 cut(s) 341, 432, 1824, 2169
BslI CCNNNNNNNGG 9 cut(s) 174, 939, 2151, 2274, 2309, 3233, 3431, 3432, 3433
BsmBI CGTCTC 1 cut(s) 68
BsmFI GGGAC 4 cut(s) 341, 432, 1824, 2169
BsmI GAATGC 3 cut(s) 195, 706, 3175
BsnI GGCC 7 cut(s) 5, 493, 1256, 2336, 2900, 3232, 3862
Bso31I GGTCTC 5 cut(s) 419, 799, 1739, 2379, 3117
BsoBI CYCGRG 3 cut(s) 112, 932, 3709
Bsp119I TTCGAA 1 cut(s) 122
Bsp1286I GDGCHC 3 cut(s) 503, 2217, 2613
Bsp1720I GCTNAGC 1 cut(s) 155
Bsp19I CCATGG 1 cut(s) 2145
Bsp68I TCGCGA 1 cut(s) 375
BspACI CCGC 8 cut(s) 6, 9, 88, 228, 325, 1765, 1784, 3773
BspANI GGCC 7 cut(s) 5, 493, 1256, 2336, 2900, 3232, 3862
BspDI ATCGAT 1 cut(s) 2451
BspFNI CGCG 1 cut(s) 375
BspHI TCATGA 1 cut(s) 3346
BspMI ACCTGC 4 cut(s) 727, 2294, 2551, 3638
BspPI GGATC 5 cut(s) 425, 765, 2389, 2491, 3181
BspQI GCTCTTC 1 cut(s) 244
BspT104I TTCGAA 1 cut(s) 122
BspT107I GGYRCC 3 cut(s) 1614, 2277, 2992
BspTI CTTAAG 1 cut(s) 3197
BspTNI GGTCTC 5 cut(s) 419, 799, 1739, 2379, 3117
BsrDI GCAATG 3 cut(s) 139, 269, 1284
BsrFI RCCGGY 1 cut(s) 11
BssAI RCCGGY 1 cut(s) 11
BssSI CACGAG 2 cut(s) 752, 1410
BssT1I CCWWGG 5 cut(s) 1964, 2145, 2208, 2268, 3227
Bst2BI CACGAG 2 cut(s) 752, 1410
Bst2UI CCWGG 4 cut(s) 990, 2877, 3234, 3635
Bst6I CTCTTC 4 cut(s) 244, 1237, 2111, 2673
BstAFI CTTAAG 1 cut(s) 3197
BstAPI GCANNNNNTGC 1 cut(s) 697
BstBI TTCGAA 1 cut(s) 122
BstC8I GCNNGC 7 cut(s) 92, 491, 503, 1373, 3273, 3572, 3593
BstDSI CCRYGG 2 cut(s) 202, 2145
BstEII GGTNACC 1 cut(s) 14
BstF5I GGATG 4 cut(s) 660, 1815, 2872, 3802
BstFNI CGCG 1 cut(s) 375
BstH2I RGCGCY 1 cut(s) 1670
BstHHI GCGC 3 cut(s) 269, 1669, 3392
BstMCI CGRYCG 1 cut(s) 2451
BstMWI GCNNNNNNNGC 5 cut(s) 697, 728, 2486, 3272, 3398
BstNI CCWGG 4 cut(s) 990, 2877, 3234, 3635
BstNSI RCATGY 5 cut(s) 94, 344, 999, 1514, 2003
BstPI GGTNACC 1 cut(s) 14
BstSCI CCNGG 7 cut(s) 931, 932, 988, 2875, 3232, 3499, 3633
BstUI CGCG 1 cut(s) 375
BstV1I GCAGC 4 cut(s) 601, 1295, 2501, 2527
BstV2I GAAGAC 4 cut(s) 1104, 1847, 2019, 2256
BstX2I RGATCY 2 cut(s) 757, 2394
BstYI RGATCY 2 cut(s) 757, 2394
Bsu15I ATCGAT 1 cut(s) 2451
Bsu36I CCTNAGG 1 cut(s) 3807
BsuI GTATCC 3 cut(s) 526, 1094, 3430
BsuRI GGCC 7 cut(s) 5, 493, 1256, 2336, 2900, 3232, 3862
BsuTUI ATCGAT 1 cut(s) 2451
BtgI CCRYGG 2 cut(s) 202, 2145
BtgZI GCGATG 1 cut(s) 1218
BtrI CACGTC 1 cut(s) 3506
BtsCI GGATG 4 cut(s) 660, 1815, 2872, 3802
BtsI GCAGTG 1 cut(s) 698
BtsIMutI CAGTG 9 cut(s) 184, 698, 907, 1176, 2321, 2384, 2410, 3153, 3673
BtuMI TCGCGA 1 cut(s) 375
BveI ACCTGC 4 cut(s) 727, 2294, 2551, 3638
Cac8I GCNNGC 7 cut(s) 92, 491, 503, 1373, 3273, 3572, 3593
CaiI CAGNNNCTG 4 cut(s) 162, 719, 1868, 3653
CciI TCATGA 1 cut(s) 3346
CfoI GCGC 3 cut(s) 269, 1669, 3392
Cfr10I RCCGGY 1 cut(s) 11
Cfr13I GGNCC 4 cut(s) 328, 492, 835, 3631
Cfr9I CCCGGG 1 cut(s) 932
ClaI ATCGAT 1 cut(s) 2451
CseI GACGC 1 cut(s) 138
Csp6I GTAC 8 cut(s) 338, 356, 667, 2265, 2278, 2283, 2360, 3368
CviQI GTAC 8 cut(s) 338, 356, 667, 2265, 2278, 2283, 2360, 3368
DraI TTTAAA 1 cut(s) 1467
DrdI GACNNNNNNGTC 2 cut(s) 791, 833
DseDI GACNNNNNNGTC 2 cut(s) 791, 833
EaeI YGGCCR 1 cut(s) 3
Eam1104I CTCTTC 4 cut(s) 244, 1237, 2111, 2673
EarI CTCTTC 4 cut(s) 244, 1237, 2111, 2673
Ecl136II GAGCTC 2 cut(s) 501, 2611
Eco130I CCWWGG 5 cut(s) 1964, 2145, 2208, 2268, 3227
Eco147I AGGCCT 1 cut(s) 2900
Eco24I GRGCYC 2 cut(s) 503, 2613
Eco31I GGTCTC 5 cut(s) 419, 799, 1739, 2379, 3117
Eco32I GATATC 1 cut(s) 561
Eco47I GGWCC 3 cut(s) 328, 835, 3631
Eco53kI GAGCTC 2 cut(s) 501, 2611
Eco57I CTGAAG 2 cut(s) 83, 1046
Eco81I CCTNAGG 1 cut(s) 3807
Eco88I CYCGRG 3 cut(s) 112, 932, 3709
Eco91I GGTNACC 1 cut(s) 14
EcoICRI GAGCTC 2 cut(s) 501, 2611
EcoO109I RGGNCCY 2 cut(s) 328, 3631
EcoO65I GGTNACC 1 cut(s) 14
EcoRII CCWGG 4 cut(s) 988, 2875, 3232, 3633
EcoRV GATATC 1 cut(s) 561
EcoT14I CCWWGG 5 cut(s) 1964, 2145, 2208, 2268, 3227
EcoT22I ATGCAT 1 cut(s) 3362
EcoT38I GRGCYC 2 cut(s) 503, 2613
ErhI CCWWGG 5 cut(s) 1964, 2145, 2208, 2268, 3227
Esp3I CGTCTC 1 cut(s) 68
FalI AAGNNNNNCTT 6 cut(s) 1022, 1054, 1734, 1766, 2885, 2917
FaqI GGGAC 4 cut(s) 341, 432, 1824, 2169
FauI CCCGC 3 cut(s) 235, 318, 3766
FbaI TGATCA 1 cut(s) 3672
FblI GTMKAC 1 cut(s) 2501
Fnu4HI GCNGC 7 cut(s) 6, 9, 590, 1309, 1784, 2490, 2541
FokI GGATG 4 cut(s) 667, 1802, 2859, 3789
FriOI GRGCYC 2 cut(s) 503, 2613
Fsp4HI GCNGC 7 cut(s) 6, 9, 590, 1309, 1784, 2490, 2541
FspBI CTAG 8 cut(s) 615, 732, 1191, 1965, 2042, 2664, 2940, 3871
GlaI GCGC 3 cut(s) 268, 1668, 3391
GluI GCNGC 7 cut(s) 6, 9, 590, 1309, 1784, 2490, 2541
GsaI CCCAGC 2 cut(s) 171, 3657
GsuI CTGGAG 2 cut(s) 592, 1011
HaeII RGCGCY 1 cut(s) 1670
HaeIII GGCC 7 cut(s) 5, 493, 1256, 2336, 2900, 3232, 3862
HapII CCGG 4 cut(s) 12, 27, 933, 3501
HgaI GACGC 1 cut(s) 138
HhaI GCGC 3 cut(s) 269, 1669, 3392
Hin6I GCGC 3 cut(s) 267, 1667, 3390
HinP1I GCGC 3 cut(s) 267, 1667, 3390
HincII GTYRAC 3 cut(s) 312, 2235, 2242
HindII GTYRAC 3 cut(s) 312, 2235, 2242
HindIII AAGCTT 1 cut(s) 252
HpaI GTTAAC 1 cut(s) 312
HpaII CCGG 4 cut(s) 12, 27, 933, 3501
HphI GGTGA 6 cut(s) 869, 905, 1953, 2018, 2068, 3248
Hpy166II GTNNAC 8 cut(s) 248, 312, 1440, 2235, 2242, 2502, 2937, 3258
Hpy8I GTNNAC 8 cut(s) 248, 312, 1440, 2235, 2242, 2502, 2937, 3258
HpyCH4IV ACGT 2 cut(s) 336, 3505
HpyF10VI GCNNNNNNNGC 5 cut(s) 697, 728, 2486, 3272, 3398
HpySE526I ACGT 2 cut(s) 336, 3505
HspAI GCGC 3 cut(s) 267, 1667, 3390
KflI GGGWCCC 1 cut(s) 328
KpnI GGTACC 1 cut(s) 2281
Ksp22I TGATCA 1 cut(s) 3672
KspAI GTTAAC 1 cut(s) 312
LguI GCTCTTC 1 cut(s) 244
LmnI GCTCC 8 cut(s) 46, 52, 952, 992, 1133, 2834, 3038, 3178
Lsp1109I GCAGC 4 cut(s) 601, 1295, 2501, 2527
LweI GCATC 3 cut(s) 645, 1260, 1384
MaeI CTAG 8 cut(s) 615, 732, 1191, 1965, 2042, 2664, 2940, 3871
MaeII ACGT 2 cut(s) 336, 3505
MfeI CAATTG 1 cut(s) 1770
MflI RGATCY 2 cut(s) 757, 2394
MhlI GDGCHC 3 cut(s) 503, 2217, 2613
MlyI GAGTC 5 cut(s) 1165, 1806, 1921, 3253, 3611
MmeI TCCRAC 3 cut(s) 458, 1519, 1915
Mph1103I ATGCAT 1 cut(s) 3362
MroXI GAANNNNTTC 3 cut(s) 218, 1033, 2900
MslI CAYNNNNRTG 3 cut(s) 912, 1863, 2004
MspA1I CMGCKG 2 cut(s) 230, 2132
MspCI CTTAAG 1 cut(s) 3197
MspI CCGG 4 cut(s) 12, 27, 933, 3501
MspR9I CCNGG 7 cut(s) 933, 934, 990, 2877, 3234, 3501, 3635
MunI CAATTG 1 cut(s) 1770
Mva1269I GAATGC 3 cut(s) 195, 706, 3175
MvaI CCWGG 4 cut(s) 990, 2877, 3234, 3635
MvnI CGCG 1 cut(s) 375
MwoI GCNNNNNNNGC 5 cut(s) 697, 728, 2486, 3272, 3398
NciI CCSGG 3 cut(s) 933, 934, 3501
NcoI CCATGG 1 cut(s) 2145
NmeAIII GCCGAG 3 cut(s) 1282, 2397, 3652
NmuCI GTSAC 5 cut(s) 857, 1162, 1171, 1820, 1959
NruI TCGCGA 1 cut(s) 375
NsiI ATGCAT 1 cut(s) 3362
NspI RCATGY 5 cut(s) 94, 344, 999, 1514, 2003
NspV TTCGAA 1 cut(s) 122
OliI CACNNNNGTG 1 cut(s) 912
PaeI GCATGC 1 cut(s) 94
PaeR7I CTCGAG 1 cut(s) 3709
PagI TCATGA 1 cut(s) 3346
PaqCI CACCTGC 2 cut(s) 727, 3638
PceI AGGCCT 1 cut(s) 2900
PciI ACATGT 2 cut(s) 1510, 1999
PciSI GCTCTTC 1 cut(s) 244
PcsI WCGNNNNNNNCGW 1 cut(s) 119
PctI GAATGC 3 cut(s) 195, 706, 3175
PdmI GAANNNNTTC 3 cut(s) 218, 1033, 2900
PflMI CCANNNNNTGG 2 cut(s) 174, 2274
PfoI TCCNGGA 3 cut(s) 988, 2875, 3499
PkrI GCNGC 7 cut(s) 7, 10, 591, 1310, 1785, 2491, 2542
Ple19I CGATCG 1 cut(s) 2451
PleI GAGTC 5 cut(s) 1164, 1806, 1920, 3253, 3611
PpsI GAGTC 5 cut(s) 1164, 1806, 1920, 3253, 3611
PpuMI RGGWCCY 2 cut(s) 328, 3631
PscI ACATGT 2 cut(s) 1510, 1999
PshBI ATTAAT 3 cut(s) 105, 1056, 2511
Psp124BI GAGCTC 2 cut(s) 503, 2613
Psp5II RGGWCCY 2 cut(s) 328, 3631
Psp6I CCWGG 4 cut(s) 988, 2875, 3232, 3633
PspEI GGTNACC 1 cut(s) 14
PspFI CCCAGC 2 cut(s) 167, 3653
PspGI CCWGG 4 cut(s) 988, 2875, 3232, 3633
PspPI GGNCC 4 cut(s) 328, 492, 835, 3631
PspPPI RGGWCCY 2 cut(s) 328, 3631
PsrI GAACNNNNNNTAC 2 cut(s) 1379, 1411
PstNI CAGNNNCTG 4 cut(s) 162, 719, 1868, 3653
PsuI RGATCY 2 cut(s) 757, 2394
PvuI CGATCG 1 cut(s) 2451
PvuII CAGCTG 1 cut(s) 2132
RruI TCGCGA 1 cut(s) 375
RsaI GTAC 8 cut(s) 339, 357, 668, 2266, 2279, 2284, 2361, 3369
RsaNI GTAC 8 cut(s) 338, 356, 667, 2265, 2278, 2283, 2360, 3368
RseI CAYNNNNRTG 3 cut(s) 912, 1863, 2004
SacI GAGCTC 2 cut(s) 503, 2613
SapI GCTCTTC 1 cut(s) 244
SatI GCNGC 7 cut(s) 6, 9, 590, 1309, 1784, 2490, 2541
Sau96I GGNCC 4 cut(s) 328, 492, 835, 3631
ScaI AGTACT 1 cut(s) 2361
SchI GAGTC 5 cut(s) 1165, 1806, 1921, 3253, 3611
ScrFI CCNGG 7 cut(s) 933, 934, 990, 2877, 3234, 3501, 3635
SduI GDGCHC 3 cut(s) 503, 2217, 2613
SfaNI GCATC 3 cut(s) 645, 1260, 1384
Sfr274I CTCGAG 1 cut(s) 3709
SfuI TTCGAA 1 cut(s) 122
SinI GGWCC 3 cut(s) 328, 835, 3631
SlaI CTCGAG 1 cut(s) 3709
SmaI CCCGGG 1 cut(s) 934
SmiMI CAYNNNNRTG 3 cut(s) 912, 1863, 2004
SmlI CTYRAG 4 cut(s) 1924, 3197, 3511, 3709
SmoI CTYRAG 4 cut(s) 1924, 3197, 3511, 3709
SpeI ACTAGT 1 cut(s) 2939
SphI GCATGC 1 cut(s) 94
SseBI AGGCCT 1 cut(s) 2900
SsiI CCGC 8 cut(s) 6, 9, 88, 228, 325, 1765, 1784, 3773
SspMI CTAG 8 cut(s) 615, 732, 1191, 1965, 2042, 2664, 2940, 3871
SstI GAGCTC 2 cut(s) 503, 2613
StuI AGGCCT 1 cut(s) 2900
StyD4I CCNGG 7 cut(s) 931, 932, 988, 2875, 3232, 3499, 3633
StyI CCWWGG 5 cut(s) 1964, 2145, 2208, 2268, 3227
TaiI ACGT 2 cut(s) 339, 3508
TaqI TCGA 8 cut(s) 122, 318, 1240, 2050, 2217, 2451, 3710, 3758
TaqII GACCGA 1 cut(s) 443
TatI WGTACW 3 cut(s) 355, 2359, 3367
TauI GCSGC 3 cut(s) 8, 11, 1786
TscAI CASTG 9 cut(s) 191, 698, 914, 1176, 2321, 2384, 2410, 3160, 3673
TseFI GTSAC 5 cut(s) 857, 1162, 1171, 1820, 1959
TseI GCWGC 4 cut(s) 589, 1308, 2489, 2540
Tsp45I GTSAC 5 cut(s) 857, 1162, 1171, 1820, 1959
TspGWI ACGGA 6 cut(s) 191, 398, 814, 1146, 1307, 1808
TspMI CCCGGG 1 cut(s) 932
TspRI CASTG 9 cut(s) 191, 698, 914, 1176, 2321, 2384, 2410, 3160, 3673
Van91I CCANNNNNTGG 2 cut(s) 174, 2274
Vha464I CTTAAG 1 cut(s) 3197
VpaK11BI GGWCC 3 cut(s) 328, 835, 3631
VspI ATTAAT 3 cut(s) 105, 1056, 2511
XapI RAATTY 8 cut(s) 146, 350, 742, 979, 1675, 2221, 3009, 3541
XceI RCATGY 5 cut(s) 94, 344, 999, 1514, 2003
XhoI CTCGAG 1 cut(s) 3709
XmaI CCCGGG 1 cut(s) 932
XmaJI CCTAGG 1 cut(s) 1964
XmiI GTMKAC 1 cut(s) 2501
XmnI GAANNNNTTC 3 cut(s) 218, 1033, 2900
XspI CTAG 8 cut(s) 615, 732, 1191, 1965, 2042, 2664, 2940, 3871
ZrmI AGTACT 1 cut(s) 2361
Zsp2I ATGCAT 1 cut(s) 3362
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.