pycom09g05850

OTU domain-containing protein DDB_G0284757-like

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
pyrus_communis
Chr9
OG0002298
Physical Location & Seq
Reverse (-)
4351218 .. 4354253
3036 bp
Loading structure...
UTR
Exon/CDS
Intron
N/A

Sequence Viewer

Length: 606 bp
ATGAATGGAAGCTACAGCAATGCGAGTGCGAGTTCAAGCTCGAGTTTAGATAGCAGCAATCATGATACAGAGGATGATCAAGCCATTGCAACCATCTTGGCAGAAGAGGAAAAGGTCAAAGTTGATCGCAGGCTTGGGAAGAGACTGTCCCACTTAGATTCGATTCCGCACACTCCCCGCGTAAATGGGGAGATACCTGACGTGAATGATGCGACCCAAGACCATGAACGCCTGTCGGCAAGATTGGCAACATATGATTTATCTGAACTGCAGATCGAGGGCGATGGAAATTGTCAGTTTCGAGCAATAGCAGATCAATTGTTTCGGAATCCAGAATACCACAAGCATGTAAGAAAACAGGTGATAAAGCAGCTAAAGCATCACAAAAAACTCTATGAAGCTTACGTCCCGATGAAATACAGACGCTACCTGAGGAAGATGAAAAAAACCGGGGAGTGGGGAGATCGATCTTACACTACAGGCAGCTGCAGATCGATTCGGAGCCAAGATTTGTTTAATCACCTCTTTTCGAGACACTTGCTACATCGAGATTCTTCCAAAAGATGGGAATCCTACCCTAGAGCTTTGGCTGAGCTTTTGGAGTGA
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
No pathways identified.
Pfam Domains
Protein Families
OTU

Protein Analysis

202

Amino Acids

23.34

Weight (kDa)

8.98

Isoelectric Point (pI)

52.19

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
OTU PF02338 94 - 155 2.3e-09 OTU-like cysteine protease
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

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Publication-ready
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Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AccB7I CCANNNNNTGG 1 cut(s) 564
AccII CGCG 1 cut(s) 180
AciI CCGC 2 cut(s) 167, 178
AfiI CCNNNNNNNGG 2 cut(s) 456, 564
AgsI TTSAA 1 cut(s) 36
AjiI CACGTC 1 cut(s) 202
AluBI AGCT 7 cut(s) 12, 39, 373, 401, 486, 584, 595
AluI AGCT 7 cut(s) 12, 39, 373, 401, 486, 584, 595
Alw26I GTCTC 2 cut(s) 136, 526
Ama87I CYCGRG 1 cut(s) 40
ApeKI GCWGC 4 cut(s) 54, 370, 483, 486
AsuC2I CCSGG 1 cut(s) 451
AsuHPI GGTGA 2 cut(s) 373, 512
AvaI CYCGRG 1 cut(s) 40
AxyI CCTNAGG 1 cut(s) 431
BbvI GCAGC 4 cut(s) 66, 382, 473, 495
BccI CCATC 3 cut(s) 101, 278, 558
BcgI CGANNNNNNTGC 2 cut(s) 520, 554
BclI TGATCA 1 cut(s) 76
BcnI CCSGG 1 cut(s) 451
BcoDI GTCTC 2 cut(s) 136, 526
BfaI CTAG 1 cut(s) 579
BfmI CTRYAG 4 cut(s) 13, 269, 477, 487
BisI GCNGC 4 cut(s) 55, 371, 484, 487
BlpI GCTNAGC 1 cut(s) 591
BlsI GCNGC 4 cut(s) 56, 372, 485, 488
Bme1390I CCNGG 1 cut(s) 451
BmeT110I CYCGRG 1 cut(s) 40
BmgBI CACGTC 1 cut(s) 202
BmiI GGNNCC 1 cut(s) 503
BmrFI CCNGG 1 cut(s) 451
BmsI GCATC 2 cut(s) 199, 388
Bpu1102I GCTNAGC 1 cut(s) 591
BpuMI CCSGG 1 cut(s) 451
Bsa29I ATCGAT 2 cut(s) 466, 494
BsaBI GATNNNNATC 1 cut(s) 568
BsaJI CCNNGG 1 cut(s) 450
Bsc4I CCNNNNNNNGG 2 cut(s) 456, 564
Bse21I CCTNAGG 1 cut(s) 431
Bse3DI GCAATG 2 cut(s) 25, 84
Bse8I GATNNNNATC 1 cut(s) 568
BseCI ATCGAT 2 cut(s) 466, 494
BseDI CCNNGG 1 cut(s) 450
BseGI GGATG 1 cut(s) 79
BseJI GATNNNNATC 1 cut(s) 568
BseLI CCNNNNNNNGG 2 cut(s) 456, 564
BseMI GCAATG 2 cut(s) 25, 84
BseMII CTCAG 2 cut(s) 422, 582
BseXI GCAGC 4 cut(s) 66, 382, 473, 495
Bsh1236I CGCG 1 cut(s) 180
BshVI ATCGAT 2 cut(s) 466, 494
BsiHKCI CYCGRG 1 cut(s) 40
BsiSI CCGG 1 cut(s) 450
BslFI GGGAC 2 cut(s) 133, 392
BslI CCNNNNNNNGG 2 cut(s) 456, 564
BsmAI GTCTC 2 cut(s) 136, 526
BsmFI GGGAC 2 cut(s) 133, 392
BsoBI CYCGRG 1 cut(s) 40
Bsp143I GATC 7 cut(s) 76, 124, 273, 313, 463, 467, 491
Bsp1720I GCTNAGC 1 cut(s) 591
BspACI CCGC 2 cut(s) 167, 178
BspCNI CTCAG 2 cut(s) 423, 583
BspDI ATCGAT 2 cut(s) 466, 494
BspFNI CGCG 1 cut(s) 180
BspHI TCATGA 1 cut(s) 61
BspLI GGNNCC 1 cut(s) 503
BspMAI CTGCAG 2 cut(s) 273, 491
BsrDI GCAATG 2 cut(s) 25, 84
BssECI CCNNGG 1 cut(s) 450
BssMI GATC 7 cut(s) 76, 124, 273, 313, 463, 467, 491
Bst4CI ACNGT 1 cut(s) 147
Bst6I CTCTTC 2 cut(s) 99, 134
BstC8I GCNNGC 1 cut(s) 131
BstDEI CTNAG 3 cut(s) 154, 431, 591
BstF5I GGATG 1 cut(s) 79
BstFNI CGCG 1 cut(s) 180
BstKTI GATC 7 cut(s) 79, 127, 276, 316, 466, 470, 494
BstMAI GTCTC 2 cut(s) 136, 526
BstMBI GATC 7 cut(s) 76, 124, 273, 313, 463, 467, 491
BstMWI GCNNNNNNNGC 2 cut(s) 245, 376
BstNSI RCATGY 1 cut(s) 350
BstSCI CCNGG 1 cut(s) 449
BstSFI CTRYAG 4 cut(s) 13, 269, 477, 487
BstUI CGCG 1 cut(s) 180
BstV1I GCAGC 4 cut(s) 66, 382, 473, 495
Bsu15I ATCGAT 2 cut(s) 466, 494
Bsu36I CCTNAGG 1 cut(s) 431
BsuTUI ATCGAT 2 cut(s) 466, 494
BtgZI GCGATG 1 cut(s) 297
BtrI CACGTC 1 cut(s) 202
BtsCI GGATG 1 cut(s) 79
Cac8I GCNNGC 1 cut(s) 131
CciI TCATGA 1 cut(s) 61
ClaI ATCGAT 2 cut(s) 466, 494
CseI GACGC 1 cut(s) 432
CviAII CATG 3 cut(s) 62, 224, 347
DdeI CTNAG 3 cut(s) 154, 431, 591
DpnI GATC 7 cut(s) 78, 126, 275, 315, 465, 469, 493
DpnII GATC 7 cut(s) 76, 124, 273, 313, 463, 467, 491
Eam1104I CTCTTC 2 cut(s) 99, 134
EarI CTCTTC 2 cut(s) 99, 134
Eco81I CCTNAGG 1 cut(s) 431
Eco88I CYCGRG 1 cut(s) 40
FaeI CATG 3 cut(s) 65, 227, 350
FaiI YATR 6 cut(s) 63, 225, 253, 255, 348, 396
FaqI GGGAC 2 cut(s) 133, 392
FatI CATG 3 cut(s) 61, 223, 346
FauI CCCGC 1 cut(s) 185
FauNDI CATATG 1 cut(s) 253
FbaI TGATCA 1 cut(s) 76
Fnu4HI GCNGC 4 cut(s) 55, 371, 484, 487
FokI GGATG 1 cut(s) 86
Fsp4HI GCNGC 4 cut(s) 55, 371, 484, 487
FspBI CTAG 1 cut(s) 579
GluI GCNGC 4 cut(s) 55, 371, 484, 487
HapII CCGG 1 cut(s) 450
HgaI GACGC 1 cut(s) 432
Hin1II CATG 3 cut(s) 65, 227, 350
HindIII AAGCTT 1 cut(s) 399
HinfI GANTC 6 cut(s) 158, 163, 328, 496, 551, 569
HpaII CCGG 1 cut(s) 450
HphI GGTGA 2 cut(s) 373, 512
Hpy188I TCNGA 3 cut(s) 265, 327, 501
Hpy188III TCNNGA 5 cut(s) 62, 332, 409, 531, 548
HpyCH4III ACNGT 1 cut(s) 147
HpyCH4IV ACGT 2 cut(s) 201, 405
HpyCH4V TGCA 3 cut(s) 89, 271, 489
HpyF10VI GCNNNNNNNGC 2 cut(s) 245, 376
HpyF3I CTNAG 3 cut(s) 154, 431, 591
HpySE526I ACGT 2 cut(s) 201, 405
Hsp92II CATG 3 cut(s) 65, 227, 350
Ksp22I TGATCA 1 cut(s) 76
Kzo9I GATC 7 cut(s) 76, 124, 273, 313, 463, 467, 491
LmnI GCTCC 1 cut(s) 501
LpnPI CCDG 8 cut(s) 115, 210, 245, 344, 345, 443, 463, 465
Lsp1109I GCAGC 4 cut(s) 66, 382, 473, 495
LweI GCATC 2 cut(s) 199, 388
MaeI CTAG 1 cut(s) 579
MaeII ACGT 2 cut(s) 201, 405
MalI GATC 7 cut(s) 78, 126, 275, 315, 465, 469, 493
MboI GATC 7 cut(s) 76, 124, 273, 313, 463, 467, 491
MboII GAAGA 4 cut(s) 116, 151, 448, 546
MfeI CAATTG 1 cut(s) 317
MluCI AATT 2 cut(s) 289, 317
MnlI CCTC 5 cut(s) 64, 100, 271, 426, 533
MseI TTAA 1 cut(s) 516
MslI CAYNNNNRTG 1 cut(s) 345
MspA1I CMGCKG 1 cut(s) 486
MspI CCGG 1 cut(s) 450
MspR9I CCNGG 1 cut(s) 451
MunI CAATTG 1 cut(s) 317
MvnI CGCG 1 cut(s) 180
MwoI GCNNNNNNNGC 2 cut(s) 245, 376
NciI CCSGG 1 cut(s) 451
NdeI CATATG 1 cut(s) 253
NdeII GATC 7 cut(s) 76, 124, 273, 313, 463, 467, 491
NlaIII CATG 3 cut(s) 65, 227, 350
NlaIV GGNNCC 1 cut(s) 503
NspI RCATGY 1 cut(s) 350
PaeR7I CTCGAG 1 cut(s) 40
PagI TCATGA 1 cut(s) 61
PfeI GAWTC 6 cut(s) 158, 163, 328, 496, 551, 569
PflMI CCANNNNNTGG 1 cut(s) 564
PkrI GCNGC 4 cut(s) 56, 372, 485, 488
PspN4I GGNNCC 1 cut(s) 503
PspXI VCTCGAGB 1 cut(s) 40
PstI CTGCAG 2 cut(s) 273, 491
PvuII CAGCTG 1 cut(s) 486
RseI CAYNNNNRTG 1 cut(s) 345
SaqAI TTAA 1 cut(s) 516
SatI GCNGC 4 cut(s) 55, 371, 484, 487
Sau3AI GATC 7 cut(s) 76, 124, 273, 313, 463, 467, 491
ScrFI CCNGG 1 cut(s) 451
SfaNI GCATC 2 cut(s) 199, 388
SfcI CTRYAG 4 cut(s) 13, 269, 477, 487
Sfr274I CTCGAG 1 cut(s) 40
SlaI CTCGAG 1 cut(s) 40
SmiMI CAYNNNNRTG 1 cut(s) 345
SmlI CTYRAG 1 cut(s) 40
SmoI CTYRAG 1 cut(s) 40
Sse9I AATT 2 cut(s) 289, 317
SsiI CCGC 2 cut(s) 167, 178
SspMI CTAG 1 cut(s) 579
StyD4I CCNGG 1 cut(s) 449
TaaI ACNGT 1 cut(s) 147
TaiI ACGT 2 cut(s) 204, 408
TaqI TCGA 8 cut(s) 41, 161, 276, 301, 466, 494, 530, 547
TasI AATT 2 cut(s) 289, 317
TfiI GAWTC 6 cut(s) 158, 163, 328, 496, 551, 569
Tru1I TTAA 1 cut(s) 516
Tru9I TTAA 1 cut(s) 516
TseI GCWGC 4 cut(s) 54, 370, 483, 486
TspDTI ATGAA 5 cut(s) 17, 240, 411, 428, 455
Van91I CCANNNNNTGG 1 cut(s) 564
XceI RCATGY 1 cut(s) 350
XhoI CTCGAG 1 cut(s) 40
XspI CTAG 1 cut(s) 579
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.