pycom09g08280

F-box-like

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
pyrus_communis
Chr9
OG0004624
Physical Location & Seq
Forward (+)
6351446 .. 6354590
3145 bp
Loading structure...
UTR
Exon/CDS
Intron
N/A

Sequence Viewer

Length: 879 bp
ATGGAAATTCAAGTGAAACTAAGGAGAGGAAAACAGGAGCCTTGTCTTTCTTCCTCCACTAGAACATTCAGCAGAAAATTCGAGCAAATTTATAAAATAAAAAAGAAACCCTTTGAGGCTGAAAAGCTCACTCCTTCCTCTCAGTCTCTCAGCAAGTGCAGAACCCTAATGACAGATAACAATCAAAATGACAGTTTAATGTCAGTTGGTAGTTCGTTCGGAAAGCTTCCTGATCATTTGCTAATAGAAATTTTCATCCGAGTTCCTGTCTCAGAGTGGGCACAAATCTCATGTGTTAAAAAACAGTGGGCCAATTTGTTTCGCGAAGAATTCTTGTGGCAAGCTGCTATTGCTATGACATTTCCTTTGGCTAGCCAAGCTAAAAGGTGGCCGGGACCTATCCCTCGGGGATTGAACAAGAGGAGATTTGAAGCTTTATATGTCAGTAAACATATCTTTGCTCTAGATGGTGAGATTGATGAGATCATGGGCCATAGTTATTTGTTTCTAAAAGAGCAGCTTGAGCGTTCAACCATGCCACCCTCTTCCAGTATACTGCACGGAACCCTCATAGATCAGTTTATTGCTTGTGGGAAATCGAGAGACATGGCTCATGATCTTGCTTCCCAGATCTGGCTGGCTGTTCTTGACAATTTAGAGGAAAACGAGCAAACTTTTCAATTACTGAAACGTCTTGCACAAGAAGCCGATGTTTTTCTTCTGTACCCGTACTCAAGATCAATCACAGTCCAATGGAGGGTGTTTGAAAAACTCTTCACAGATTTTCGCGACTGCTTCAATCATGTAGATTACTATGATGTGTTGGCTTGTGCGAAGAACAAGTTTCAGCCAATACCATCGACTTGGTTAGGTTACTGA
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
No pathways identified.
Pfam Domains
Protein Families
F-box F-box-like

Protein Analysis

293

Amino Acids

33.93

Weight (kDa)

8.8

Isoelectric Point (pI)

57.4

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
F-box-like PF12937 74 - 114 9.9e-06 F-box-like
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

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Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AanI TTATAA 1 cut(s) 93
AccI GTMKAC 1 cut(s) 553
AccII CGCG 2 cut(s) 324, 789
AcoI YGGCCR 1 cut(s) 389
AcsI RAATTY 5 cut(s) 6, 77, 87, 249, 329
AfaI GTAC 2 cut(s) 725, 731
AfiI CCNNNNNNNGG 2 cut(s) 633, 757
AgsI TTSAA 7 cut(s) 11, 415, 431, 531, 680, 767, 799
AluBI AGCT 6 cut(s) 127, 226, 344, 380, 434, 520
AluI AGCT 6 cut(s) 127, 226, 344, 380, 434, 520
Alw26I GTCTC 3 cut(s) 150, 274, 597
Ama87I CYCGRG 1 cut(s) 405
AoxI GGCC 3 cut(s) 309, 389, 490
ApeKI GCWGC 2 cut(s) 344, 517
ApoI RAATTY 5 cut(s) 6, 77, 87, 249, 329
ArsI GACNNNNNNTTYG 2 cut(s) 349, 381
AspS9I GGNCC 3 cut(s) 309, 395, 490
AsuC2I CCSGG 1 cut(s) 393
AsuHPI GGTGA 1 cut(s) 482
AsuNHI GCTAGC 1 cut(s) 371
AvaI CYCGRG 1 cut(s) 405
AvaII GGWCC 1 cut(s) 395
BaeGI GKGCMC 1 cut(s) 283
BbvI GCAGC 2 cut(s) 331, 529
BccI CCATC 2 cut(s) 461, 865
BcgI CGANNNNNNTGC 2 cut(s) 61, 95
BclI TGATCA 1 cut(s) 232
BcnI CCSGG 1 cut(s) 393
BcoDI GTCTC 3 cut(s) 150, 274, 597
BfaI CTAG 3 cut(s) 60, 372, 464
BglII AGATCT 1 cut(s) 630
BisI GCNGC 2 cut(s) 345, 518
BlsI GCNGC 2 cut(s) 346, 519
Bme1390I CCNGG 1 cut(s) 393
Bme18I GGWCC 1 cut(s) 395
BmeT110I CYCGRG 1 cut(s) 405
BmgT120I GGNCC 3 cut(s) 309, 395, 490
BmiI GGNNCC 3 cut(s) 39, 396, 565
BmrFI CCNGG 1 cut(s) 393
BmtI GCTAGC 1 cut(s) 375
BpuEI CTTGAG 2 cut(s) 542, 718
BpuMI CCSGG 1 cut(s) 393
BsaBI GATNNNNATC 1 cut(s) 180
BsaJI CCNNGG 1 cut(s) 404
Bsc4I CCNNNNNNNGG 2 cut(s) 633, 757
Bse1I ACTGG 1 cut(s) 549
Bse8I GATNNNNATC 1 cut(s) 180
BseDI CCNNGG 1 cut(s) 404
BseGI GGATG 1 cut(s) 255
BseJI GATNNNNATC 1 cut(s) 180
BseLI CCNNNNNNNGG 2 cut(s) 633, 757
BseMII CTCAG 3 cut(s) 155, 163, 285
BseNI ACTGG 1 cut(s) 549
BseRI GAGGAG 1 cut(s) 436
BseSI GKGCMC 1 cut(s) 283
BseXI GCAGC 2 cut(s) 331, 529
BsgI GTGCAG 2 cut(s) 178, 542
Bsh1236I CGCG 2 cut(s) 324, 789
BshFI GGCC 3 cut(s) 311, 391, 492
BsiHKCI CYCGRG 1 cut(s) 405
BsiSI CCGG 1 cut(s) 392
BslFI GGGAC 1 cut(s) 408
BslI CCNNNNNNNGG 2 cut(s) 633, 757
BsmAI GTCTC 3 cut(s) 150, 274, 597
BsmFI GGGAC 1 cut(s) 408
BsnI GGCC 3 cut(s) 311, 391, 492
BsoBI CYCGRG 1 cut(s) 405
Bsp1286I GDGCHC 1 cut(s) 283
Bsp143I GATC 6 cut(s) 232, 483, 574, 616, 630, 737
Bsp68I TCGCGA 2 cut(s) 324, 789
BspANI GGCC 3 cut(s) 311, 391, 492
BspCNI CTCAG 3 cut(s) 154, 162, 284
BspFNI CGCG 2 cut(s) 324, 789
BspHI TCATGA 1 cut(s) 613
BspLI GGNNCC 3 cut(s) 39, 396, 565
BspOI GCTAGC 1 cut(s) 375
BsrI ACTGG 1 cut(s) 549
BssECI CCNNGG 1 cut(s) 404
BssMI GATC 6 cut(s) 232, 483, 574, 616, 630, 737
BssNAI GTATAC 1 cut(s) 554
Bst1107I GTATAC 1 cut(s) 554
Bst4CI ACNGT 3 cut(s) 194, 306, 748
Bst6I CTCTTC 2 cut(s) 550, 779
BstC8I GCNNGC 3 cut(s) 342, 373, 639
BstDEI CTNAG 4 cut(s) 20, 141, 149, 271
BstF5I GGATG 1 cut(s) 255
BstFNI CGCG 2 cut(s) 324, 789
BstKTI GATC 6 cut(s) 235, 486, 577, 619, 633, 740
BstMAI GTCTC 3 cut(s) 150, 274, 597
BstMBI GATC 6 cut(s) 232, 483, 574, 616, 630, 737
BstMWI GCNNNNNNNGC 4 cut(s) 350, 377, 523, 704
BstSCI CCNGG 1 cut(s) 391
BstSLI GKGCMC 1 cut(s) 283
BstUI CGCG 2 cut(s) 324, 789
BstV1I GCAGC 2 cut(s) 331, 529
BstX2I RGATCY 1 cut(s) 630
BstXI CCANNNNNNTGG 1 cut(s) 864
BstYI RGATCY 1 cut(s) 630
BstZ17I GTATAC 1 cut(s) 554
BsuRI GGCC 3 cut(s) 311, 391, 492
BtsCI GGATG 1 cut(s) 255
BtsIMutI CAGTG 1 cut(s) 311
BtuMI TCGCGA 2 cut(s) 324, 789
Cac8I GCNNGC 3 cut(s) 342, 373, 639
CciI TCATGA 1 cut(s) 613
Cfr13I GGNCC 3 cut(s) 309, 395, 490
Csp6I GTAC 2 cut(s) 724, 730
CviAII CATG 6 cut(s) 291, 487, 535, 607, 614, 803
CviQI GTAC 2 cut(s) 724, 730
DdeI CTNAG 4 cut(s) 20, 141, 149, 271
DpnI GATC 6 cut(s) 234, 485, 576, 618, 632, 739
DpnII GATC 6 cut(s) 232, 483, 574, 616, 630, 737
EaeI YGGCCR 1 cut(s) 389
Eam1104I CTCTTC 2 cut(s) 550, 779
EarI CTCTTC 2 cut(s) 550, 779
Eco47I GGWCC 1 cut(s) 395
Eco88I CYCGRG 1 cut(s) 405
EcoO109I RGGNCCY 1 cut(s) 395
EcoRI GAATTC 1 cut(s) 329
FaeI CATG 6 cut(s) 294, 490, 538, 610, 617, 806
FalI AAGNNNNNCTT 4 cut(s) 95, 127, 504, 536
FaqI GGGAC 1 cut(s) 408
FatI CATG 6 cut(s) 290, 486, 534, 606, 613, 802
FbaI TGATCA 1 cut(s) 232
FblI GTMKAC 1 cut(s) 553
Fnu4HI GCNGC 2 cut(s) 345, 518
FokI GGATG 1 cut(s) 242
Fsp4HI GCNGC 2 cut(s) 345, 518
FspBI CTAG 3 cut(s) 60, 372, 464
GluI GCNGC 2 cut(s) 345, 518
HaeIII GGCC 3 cut(s) 311, 391, 492
HapII CCGG 1 cut(s) 392
Hin1II CATG 6 cut(s) 294, 490, 538, 610, 617, 806
HindIII AAGCTT 2 cut(s) 224, 432
HpaII CCGG 1 cut(s) 392
HphI GGTGA 1 cut(s) 482
Hpy166II GTNNAC 2 cut(s) 449, 554
Hpy188I TCNGA 3 cut(s) 221, 260, 274
Hpy188III TCNNGA 8 cut(s) 230, 323, 464, 600, 614, 647, 735, 788
Hpy8I GTNNAC 2 cut(s) 449, 554
HpyAV CCTTC 1 cut(s) 144
HpyCH4III ACNGT 3 cut(s) 194, 306, 748
HpyCH4IV ACGT 1 cut(s) 691
HpyCH4V TGCA 3 cut(s) 159, 559, 698
HpyF10VI GCNNNNNNNGC 4 cut(s) 350, 377, 523, 704
HpyF3I CTNAG 4 cut(s) 20, 141, 149, 271
HpySE526I ACGT 1 cut(s) 691
Hsp92II CATG 6 cut(s) 294, 490, 538, 610, 617, 806
Ksp22I TGATCA 1 cut(s) 232
Kzo9I GATC 6 cut(s) 232, 483, 574, 616, 630, 737
LmnI GCTCC 1 cut(s) 37
LpnPI CCDG 8 cut(s) 20, 243, 279, 405, 562, 619, 623, 641
Lsp1109I GCAGC 2 cut(s) 331, 529
MaeI CTAG 3 cut(s) 60, 372, 464
MaeII ACGT 1 cut(s) 691
MaeIII GTNAC 1 cut(s) 872
MalI GATC 6 cut(s) 234, 485, 576, 618, 632, 739
MboI GATC 6 cut(s) 232, 483, 574, 616, 630, 737
MboII GAAGA 6 cut(s) 42, 338, 537, 710, 766, 847
MflI RGATCY 1 cut(s) 630
MhlI GDGCHC 1 cut(s) 283
MluCI AATT 8 cut(s) 6, 77, 87, 249, 313, 329, 652, 680
MseI TTAA 2 cut(s) 197, 297
MspI CCGG 1 cut(s) 392
MspR9I CCNGG 1 cut(s) 393
MvnI CGCG 2 cut(s) 324, 789
MwoI GCNNNNNNNGC 4 cut(s) 350, 377, 523, 704
NciI CCSGG 1 cut(s) 393
NdeII GATC 6 cut(s) 232, 483, 574, 616, 630, 737
NheI GCTAGC 1 cut(s) 371
NlaIII CATG 6 cut(s) 294, 490, 538, 610, 617, 806
NlaIV GGNNCC 3 cut(s) 39, 396, 565
NruI TCGCGA 2 cut(s) 324, 789
PagI TCATGA 1 cut(s) 613
PkrI GCNGC 2 cut(s) 346, 519
PpuMI RGGWCCY 1 cut(s) 395
PsiI TTATAA 1 cut(s) 93
Psp5II RGGWCCY 1 cut(s) 395
PspN4I GGNNCC 3 cut(s) 39, 396, 565
PspPI GGNCC 3 cut(s) 309, 395, 490
PspPPI RGGWCCY 1 cut(s) 395
PsuI RGATCY 1 cut(s) 630
RruI TCGCGA 2 cut(s) 324, 789
RsaI GTAC 2 cut(s) 725, 731
RsaNI GTAC 2 cut(s) 724, 730
SaqAI TTAA 2 cut(s) 197, 297
SatI GCNGC 2 cut(s) 345, 518
Sau3AI GATC 6 cut(s) 232, 483, 574, 616, 630, 737
Sau96I GGNCC 3 cut(s) 309, 395, 490
ScrFI CCNGG 1 cut(s) 393
SduI GDGCHC 1 cut(s) 283
SinI GGWCC 1 cut(s) 395
SmlI CTYRAG 2 cut(s) 521, 733
SmoI CTYRAG 2 cut(s) 521, 733
Sse9I AATT 8 cut(s) 6, 77, 87, 249, 313, 329, 652, 680
SspMI CTAG 3 cut(s) 60, 372, 464
StyD4I CCNGG 1 cut(s) 391
TaaI ACNGT 3 cut(s) 194, 306, 748
TaiI ACGT 1 cut(s) 694
TaqI TCGA 3 cut(s) 81, 599, 860
TasI AATT 8 cut(s) 6, 77, 87, 249, 313, 329, 652, 680
Tru1I TTAA 2 cut(s) 197, 297
Tru9I TTAA 2 cut(s) 197, 297
TscAI CASTG 1 cut(s) 311
TseI GCWGC 2 cut(s) 344, 517
TspDTI ATGAA 1 cut(s) 244
TspGWI ACGGA 1 cut(s) 576
TspRI CASTG 1 cut(s) 311
VpaK11BI GGWCC 1 cut(s) 395
XapI RAATTY 5 cut(s) 6, 77, 87, 249, 329
XbaI TCTAGA 1 cut(s) 463
XmiI GTMKAC 1 cut(s) 553
XspI CTAG 3 cut(s) 60, 372, 464
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.