RchiOBHm_Chr2g0149371

F-box-like

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
rosa_chinensis
2
OG0004624
Physical Location & Seq
Reverse (-)
67224760 .. 67228245
3486 bp
Loading structure...
UTR
Exon/CDS
Intron
PRQ51877

Sequence Viewer

Length: 711 bp
ATGACAAATAATAAAGAAAATGACACCTTAACATCAGGTGACAGTTCATTTGGAAAGCTTCCTGATCATCTCCTAATAGAGATCTTCATCCGGGTTCCCGTATCTGAATGGGTACGGATATGCTGTGTAAGAAAACAGTGGGCCAATCTTTTTCGTGGAGAATGCTTGTGGCGAGCTGCTCTATCTACGACATTTCCTTTGGCTAGCAAAGCTAAAAGTTGGCCTGGACCTATCCCTCGAGGTTTGAGCAAGAGGAGATTTGCTGCTTTATATGTTAGTGAGCACATCTTTGCTCTAGATGGTGAGATTGATGAGATAGTGGGGCATACTTATTTGTTTCTAAAAGAGCAGCTTGAGCTTTCATCTATGCCTCCACCATCTGGTATTTTGCACGGTACCATAATAGATCAGTTTATTGCTTGTGGTAAATCAAGAGACATGGCTCACGAGCTTGCTTCCCAGATATGGTTGGCTGTTCTTGACAATTTAGAGGAAACCGAACACACTTTTCAAATACTTAAACGTCTCGCACAAGAGGGGGATGTTTTTCTTCCATACCCGTACTCAAGATCAATCAAAGTCCAATGGAAGGTGTTTGAAAAGCTTTTCACTGATTTTCGTGACTGCCTCAACCATGTGGATTACTATGATGTCTTGGCTAGTGCAAAGAACAAGTTTCAGCCAATACCATCTACTTGGTTAGGTCACTAA
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
No pathways identified.
Pfam Domains
Protein Families
F-box F-box-like

Protein Analysis

236

Amino Acids

27.04

Weight (kDa)

6.45

Isoelectric Point (pI)

44.97

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
F-box-like PF12937 18 - 58 5.8e-06 F-box-like
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

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Publication-ready
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Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AbsI CCTCGAGG 1 cut(s) 237
Acc65I GGTACC 1 cut(s) 395
AccB1I GGYRCC 1 cut(s) 395
AccB7I CCANNNNNTGG 1 cut(s) 380
AfaI GTAC 3 cut(s) 114, 397, 563
AfiI CCNNNNNNNGG 3 cut(s) 380, 465, 589
AgsI TTSAA 2 cut(s) 512, 599
AjnI CCWGG 1 cut(s) 223
AluBI AGCT 7 cut(s) 58, 176, 212, 352, 358, 451, 604
AluI AGCT 7 cut(s) 58, 176, 212, 352, 358, 451, 604
Alw21I GWGCWC 1 cut(s) 285
Alw26I GTCTC 2 cut(s) 429, 530
Ama87I CYCGRG 1 cut(s) 237
AoxI GGCC 2 cut(s) 141, 221
ApeKI GCWGC 3 cut(s) 176, 263, 349
ArsI GACNNNNNNTTYG 4 cut(s) 32, 64, 181, 213
Asp718I GGTACC 1 cut(s) 395
AspS9I GGNCC 2 cut(s) 141, 227
AsuC2I CCSGG 1 cut(s) 92
AsuHPI GGTGA 2 cut(s) 50, 314
AsuNHI GCTAGC 1 cut(s) 203
AvaI CYCGRG 1 cut(s) 237
AvaII GGWCC 1 cut(s) 227
BanI GGYRCC 1 cut(s) 395
BauI CACGAG 1 cut(s) 446
Bbv12I GWGCWC 1 cut(s) 285
BbvI GCAGC 3 cut(s) 163, 250, 361
BccI CCATC 3 cut(s) 293, 385, 697
BciT130I CCWGG 1 cut(s) 225
BclI TGATCA 1 cut(s) 64
BcnI CCSGG 1 cut(s) 92
BcoDI GTCTC 2 cut(s) 429, 530
BfaI CTAG 3 cut(s) 204, 296, 660
BglII AGATCT 1 cut(s) 81
BisI GCNGC 3 cut(s) 177, 264, 350
BlsI GCNGC 3 cut(s) 178, 265, 351
Bme1390I CCNGG 2 cut(s) 92, 225
Bme18I GGWCC 1 cut(s) 227
BmeT110I CYCGRG 1 cut(s) 237
BmgT120I GGNCC 2 cut(s) 141, 227
BmiI GGNNCC 2 cut(s) 96, 397
BmrFI CCNGG 2 cut(s) 92, 225
BmtI GCTAGC 1 cut(s) 207
BpuEI CTTGAG 2 cut(s) 374, 550
BpuMI CCSGG 1 cut(s) 92
BsaBI GATNNNNATC 1 cut(s) 86
Bsc4I CCNNNNNNNGG 3 cut(s) 380, 465, 589
Bse8I GATNNNNATC 1 cut(s) 86
BseBI CCWGG 1 cut(s) 225
BseGI GGATG 2 cut(s) 87, 547
BseJI GATNNNNATC 1 cut(s) 86
BseLI CCNNNNNNNGG 3 cut(s) 380, 465, 589
BseRI GAGGAG 1 cut(s) 268
BseXI GCAGC 3 cut(s) 163, 250, 361
BshFI GGCC 2 cut(s) 143, 223
BshNI GGYRCC 1 cut(s) 395
BsiHKAI GWGCWC 1 cut(s) 285
BsiHKCI CYCGRG 1 cut(s) 237
BsiSI CCGG 1 cut(s) 91
BslI CCNNNNNNNGG 3 cut(s) 380, 465, 589
BsmAI GTCTC 2 cut(s) 429, 530
BsmBI CGTCTC 1 cut(s) 530
BsmI GAATGC 1 cut(s) 167
BsnI GGCC 2 cut(s) 143, 223
BsoBI CYCGRG 1 cut(s) 237
Bsp1286I GDGCHC 1 cut(s) 285
Bsp143I GATC 4 cut(s) 64, 81, 406, 569
BspANI GGCC 2 cut(s) 143, 223
BspLI GGNNCC 2 cut(s) 96, 397
BspOI GCTAGC 1 cut(s) 207
BspT107I GGYRCC 1 cut(s) 395
BssMI GATC 4 cut(s) 64, 81, 406, 569
BssSI CACGAG 1 cut(s) 446
Bst2BI CACGAG 1 cut(s) 446
Bst2UI CCWGG 1 cut(s) 225
Bst4CI ACNGT 3 cut(s) 44, 138, 395
BstC8I GCNNGC 3 cut(s) 174, 205, 453
BstF5I GGATG 2 cut(s) 87, 547
BstKTI GATC 4 cut(s) 67, 84, 409, 572
BstMAI GTCTC 2 cut(s) 429, 530
BstMBI GATC 4 cut(s) 64, 81, 406, 569
BstMWI GCNNNNNNNGC 2 cut(s) 209, 355
BstNI CCWGG 1 cut(s) 225
BstSCI CCNGG 2 cut(s) 90, 223
BstV1I GCAGC 3 cut(s) 163, 250, 361
BstX2I RGATCY 1 cut(s) 81
BstXI CCANNNNNNTGG 1 cut(s) 696
BstYI RGATCY 1 cut(s) 81
BsuRI GGCC 2 cut(s) 143, 223
BtsCI GGATG 2 cut(s) 87, 547
BtsIMutI CAGTG 2 cut(s) 143, 609
Cac8I GCNNGC 3 cut(s) 174, 205, 453
Cfr13I GGNCC 2 cut(s) 141, 227
Csp6I GTAC 3 cut(s) 113, 396, 562
CviAII CATG 2 cut(s) 439, 635
CviQI GTAC 3 cut(s) 113, 396, 562
DpnI GATC 4 cut(s) 66, 83, 408, 571
DpnII GATC 4 cut(s) 64, 81, 406, 569
Eco47I GGWCC 1 cut(s) 227
Eco88I CYCGRG 1 cut(s) 237
EcoRII CCWGG 1 cut(s) 223
Esp3I CGTCTC 1 cut(s) 530
FaeI CATG 2 cut(s) 442, 638
FalI AAGNNNNNCTT 2 cut(s) 336, 368
FatI CATG 2 cut(s) 438, 634
FbaI TGATCA 1 cut(s) 64
Fnu4HI GCNGC 3 cut(s) 177, 264, 350
FokI GGATG 2 cut(s) 74, 554
Fsp4HI GCNGC 3 cut(s) 177, 264, 350
FspBI CTAG 3 cut(s) 204, 296, 660
GluI GCNGC 3 cut(s) 177, 264, 350
HaeIII GGCC 2 cut(s) 143, 223
HapII CCGG 1 cut(s) 91
Hin1II CATG 2 cut(s) 442, 638
HindIII AAGCTT 2 cut(s) 56, 602
HpaII CCGG 1 cut(s) 91
HphI GGTGA 2 cut(s) 50, 314
Hpy188I TCNGA 1 cut(s) 106
Hpy188III TCNNGA 7 cut(s) 62, 296, 432, 446, 479, 567, 620
HpyAV CCTTC 1 cut(s) 583
HpyCH4III ACNGT 3 cut(s) 44, 138, 395
HpyCH4IV ACGT 1 cut(s) 523
HpyCH4V TGCA 2 cut(s) 391, 665
HpyF10VI GCNNNNNNNGC 2 cut(s) 209, 355
HpySE526I ACGT 1 cut(s) 523
Hsp92II CATG 2 cut(s) 442, 638
KpnI GGTACC 1 cut(s) 399
Ksp22I TGATCA 1 cut(s) 64
Kzo9I GATC 4 cut(s) 64, 81, 406, 569
LpnPI CCDG 7 cut(s) 21, 75, 104, 210, 237, 366, 473
Lsp1109I GCAGC 3 cut(s) 163, 250, 361
MaeI CTAG 3 cut(s) 204, 296, 660
MaeII ACGT 1 cut(s) 523
MaeIII GTNAC 3 cut(s) 38, 620, 704
MalI GATC 4 cut(s) 66, 83, 408, 571
MboI GATC 4 cut(s) 64, 81, 406, 569
MboII GAAGA 2 cut(s) 76, 542
MflI RGATCY 1 cut(s) 81
MhlI GDGCHC 1 cut(s) 285
MluCI AATT 1 cut(s) 484
MnlI CCTC 7 cut(s) 233, 246, 246, 381, 484, 529, 638
MseI TTAA 2 cut(s) 29, 519
MspI CCGG 1 cut(s) 91
MspR9I CCNGG 2 cut(s) 92, 225
Mva1269I GAATGC 1 cut(s) 167
MvaI CCWGG 1 cut(s) 225
MwoI GCNNNNNNNGC 2 cut(s) 209, 355
NciI CCSGG 1 cut(s) 92
NdeII GATC 4 cut(s) 64, 81, 406, 569
NheI GCTAGC 1 cut(s) 203
NlaIII CATG 2 cut(s) 442, 638
NlaIV GGNNCC 2 cut(s) 96, 397
NmuCI GTSAC 3 cut(s) 38, 620, 704
PaeR7I CTCGAG 1 cut(s) 237
PctI GAATGC 1 cut(s) 167
PflMI CCANNNNNTGG 1 cut(s) 380
PkrI GCNGC 3 cut(s) 178, 265, 351
Psp6I CCWGG 1 cut(s) 223
PspGI CCWGG 1 cut(s) 223
PspN4I GGNNCC 2 cut(s) 96, 397
PspPI GGNCC 2 cut(s) 141, 227
PspXI VCTCGAGB 1 cut(s) 237
PsuI RGATCY 1 cut(s) 81
RsaI GTAC 3 cut(s) 114, 397, 563
RsaNI GTAC 3 cut(s) 113, 396, 562
SaqAI TTAA 2 cut(s) 29, 519
SatI GCNGC 3 cut(s) 177, 264, 350
Sau3AI GATC 4 cut(s) 64, 81, 406, 569
Sau96I GGNCC 2 cut(s) 141, 227
ScrFI CCNGG 2 cut(s) 92, 225
SduI GDGCHC 1 cut(s) 285
Sfr274I CTCGAG 1 cut(s) 237
SinI GGWCC 1 cut(s) 227
SlaI CTCGAG 1 cut(s) 237
SmlI CTYRAG 3 cut(s) 237, 353, 565
SmoI CTYRAG 3 cut(s) 237, 353, 565
Sse9I AATT 1 cut(s) 484
SspMI CTAG 3 cut(s) 204, 296, 660
StyD4I CCNGG 2 cut(s) 90, 223
TaaI ACNGT 3 cut(s) 44, 138, 395
TaiI ACGT 1 cut(s) 526
TaqI TCGA 1 cut(s) 238
TasI AATT 1 cut(s) 484
Tru1I TTAA 2 cut(s) 29, 519
Tru9I TTAA 2 cut(s) 29, 519
TscAI CASTG 2 cut(s) 143, 616
TseFI GTSAC 3 cut(s) 38, 620, 704
TseI GCWGC 3 cut(s) 176, 263, 349
Tsp45I GTSAC 3 cut(s) 38, 620, 704
TspDTI ATGAA 3 cut(s) 36, 76, 351
TspGWI ACGGA 1 cut(s) 130
TspRI CASTG 2 cut(s) 143, 616
Van91I CCANNNNNTGG 1 cut(s) 380
VpaK11BI GGWCC 1 cut(s) 227
XbaI TCTAGA 1 cut(s) 295
XhoI CTCGAG 1 cut(s) 237
XspI CTAG 3 cut(s) 204, 296, 660
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.