Rh2AG471600

F-box-like

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
rosa_samantha
Chr2A
OG0004624
Physical Location & Seq
Reverse (-)
69143956 .. 69147596
3641 bp
Loading structure...
UTR
Exon/CDS
Intron
Rh2AG471600.1

Sequence Viewer

Length: 702 bp
ATGACAACTAATAAAGAAAATGACACCTTAACATCAGGTGACAGTTCATTTGGAAAGCTTCCTGATCATCTCCTAATAGAGATCTTCATCCGGGTTCCCGTCTCTGAATGGGTACGGATATGCTGTGTAAGAAAACAGTGGGCCAATCTTTTTCGTGGAGAATGTTTGTGGCGAGCTGCTCTATCTACGACATTTCCTTTGGCTAGCAAAGCTAAAAGTTGGCCTGGACCTATCCCTCGAGGTTTGAGCAAGAGGAGATTTGCTGCTTTATATGTTAGTCAGCACATCTTTGCTCTAGATGGTGAGATTGATGAGATAGTGGGGCATACTTATTTGTTTCTAAAAGAGCAGCTTGAGCTTTCATCTATGCCTCCACCATCTGGTATTTTGCACGGTACCATAATAGATCAGTTTATTGCTTGTGGTAAATCGAGAGACATGGCTCACGAGCTTGCTTCCCAGATATGGTTGGCTGTTCTCGACAATTTAGAGGAAACCGAACACACTTTTCAAATACTTAAACGTCTCGCACAAGAGGGGGATGTAAGCTCTCTTTTCTATTTATATTACCCTTGCAACAGGTATTATTATTTTTCCTTTCCATTAATGTTCCGTGGTTTACATGCTGCTATATCATTTTGGGCAGGTTTTTCTTCCATACCCGTACTCAAGATCAATCAAAGTCCAATGGAAGGTGTTTGA
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
No pathways identified.
Pfam Domains
Protein Families
F-box F-box-like

Protein Analysis

233

Amino Acids

26.5

Weight (kDa)

6.65

Isoelectric Point (pI)

46.74

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
F-box-like PF12937 18 - 58 5.7e-06 F-box-like
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

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Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AbsI CCTCGAGG 1 cut(s) 237
Acc36I ACCTGC 1 cut(s) 635
Acc65I GGTACC 1 cut(s) 395
AccB1I GGYRCC 1 cut(s) 395
AccB7I CCANNNNNTGG 1 cut(s) 380
AfaI GTAC 3 cut(s) 114, 397, 666
AfiI CCNNNNNNNGG 3 cut(s) 380, 465, 692
AgsI TTSAA 1 cut(s) 512
AjnI CCWGG 1 cut(s) 223
AluBI AGCT 7 cut(s) 58, 176, 212, 352, 358, 451, 549
AluI AGCT 7 cut(s) 58, 176, 212, 352, 358, 451, 549
Alw26I GTCTC 3 cut(s) 106, 429, 530
Ama87I CYCGRG 1 cut(s) 237
AoxI GGCC 2 cut(s) 141, 221
ApeKI GCWGC 4 cut(s) 176, 263, 349, 626
ArsI GACNNNNNNTTYG 4 cut(s) 32, 64, 181, 213
AseI ATTAAT 1 cut(s) 605
Asp718I GGTACC 1 cut(s) 395
AspS9I GGNCC 2 cut(s) 141, 227
AsuC2I CCSGG 1 cut(s) 92
AsuHPI GGTGA 2 cut(s) 50, 314
AsuNHI GCTAGC 1 cut(s) 203
AvaI CYCGRG 1 cut(s) 237
AvaII GGWCC 1 cut(s) 227
BanI GGYRCC 1 cut(s) 395
BauI CACGAG 1 cut(s) 446
BbvI GCAGC 4 cut(s) 163, 250, 361, 613
BccI CCATC 2 cut(s) 293, 385
BciT130I CCWGG 1 cut(s) 225
BclI TGATCA 1 cut(s) 64
BcnI CCSGG 1 cut(s) 92
BcoDI GTCTC 3 cut(s) 106, 429, 530
BfaI CTAG 2 cut(s) 204, 296
BfuAI ACCTGC 1 cut(s) 635
BglII AGATCT 1 cut(s) 81
BisI GCNGC 4 cut(s) 177, 264, 350, 627
BlsI GCNGC 4 cut(s) 178, 265, 351, 628
Bme1390I CCNGG 2 cut(s) 92, 225
Bme18I GGWCC 1 cut(s) 227
BmeT110I CYCGRG 1 cut(s) 237
BmgT120I GGNCC 2 cut(s) 141, 227
BmiI GGNNCC 2 cut(s) 96, 397
BmrFI CCNGG 2 cut(s) 92, 225
BmtI GCTAGC 1 cut(s) 207
BpuEI CTTGAG 2 cut(s) 374, 653
BpuMI CCSGG 1 cut(s) 92
BsaBI GATNNNNATC 1 cut(s) 86
BsaJI CCNNGG 1 cut(s) 613
Bsc4I CCNNNNNNNGG 3 cut(s) 380, 465, 692
Bse8I GATNNNNATC 1 cut(s) 86
BseBI CCWGG 1 cut(s) 225
BseDI CCNNGG 1 cut(s) 613
BseGI GGATG 2 cut(s) 87, 547
BseJI GATNNNNATC 1 cut(s) 86
BseLI CCNNNNNNNGG 3 cut(s) 380, 465, 692
BseRI GAGGAG 1 cut(s) 268
BseXI GCAGC 4 cut(s) 163, 250, 361, 613
BshFI GGCC 2 cut(s) 143, 223
BshNI GGYRCC 1 cut(s) 395
BsiHKCI CYCGRG 1 cut(s) 237
BsiSI CCGG 1 cut(s) 91
BslI CCNNNNNNNGG 3 cut(s) 380, 465, 692
BsmAI GTCTC 3 cut(s) 106, 429, 530
BsmBI CGTCTC 2 cut(s) 106, 530
BsnI GGCC 2 cut(s) 143, 223
BsoBI CYCGRG 1 cut(s) 237
Bsp143I GATC 4 cut(s) 64, 81, 406, 672
BspANI GGCC 2 cut(s) 143, 223
BspLI GGNNCC 2 cut(s) 96, 397
BspMI ACCTGC 1 cut(s) 635
BspOI GCTAGC 1 cut(s) 207
BspT107I GGYRCC 1 cut(s) 395
BssECI CCNNGG 1 cut(s) 613
BssMI GATC 4 cut(s) 64, 81, 406, 672
BssSI CACGAG 1 cut(s) 446
Bst2BI CACGAG 1 cut(s) 446
Bst2UI CCWGG 1 cut(s) 225
Bst4CI ACNGT 3 cut(s) 44, 138, 395
BstC8I GCNNGC 3 cut(s) 174, 205, 453
BstDSI CCRYGG 1 cut(s) 613
BstF5I GGATG 2 cut(s) 87, 547
BstKTI GATC 4 cut(s) 67, 84, 409, 675
BstMAI GTCTC 3 cut(s) 106, 429, 530
BstMBI GATC 4 cut(s) 64, 81, 406, 672
BstMWI GCNNNNNNNGC 2 cut(s) 209, 355
BstNI CCWGG 1 cut(s) 225
BstNSI RCATGY 1 cut(s) 626
BstSCI CCNGG 2 cut(s) 90, 223
BstV1I GCAGC 4 cut(s) 163, 250, 361, 613
BstX2I RGATCY 1 cut(s) 81
BstYI RGATCY 1 cut(s) 81
BsuRI GGCC 2 cut(s) 143, 223
BtgI CCRYGG 1 cut(s) 613
BtsCI GGATG 2 cut(s) 87, 547
BtsIMutI CAGTG 1 cut(s) 143
BveI ACCTGC 1 cut(s) 635
Cac8I GCNNGC 3 cut(s) 174, 205, 453
Cfr13I GGNCC 2 cut(s) 141, 227
Csp6I GTAC 3 cut(s) 113, 396, 665
CviAII CATG 2 cut(s) 439, 623
CviQI GTAC 3 cut(s) 113, 396, 665
DpnI GATC 4 cut(s) 66, 83, 408, 674
DpnII GATC 4 cut(s) 64, 81, 406, 672
Eco47I GGWCC 1 cut(s) 227
Eco88I CYCGRG 1 cut(s) 237
EcoRII CCWGG 1 cut(s) 223
Esp3I CGTCTC 2 cut(s) 106, 530
FaeI CATG 2 cut(s) 442, 626
FalI AAGNNNNNCTT 2 cut(s) 336, 368
FatI CATG 2 cut(s) 438, 622
FbaI TGATCA 1 cut(s) 64
Fnu4HI GCNGC 4 cut(s) 177, 264, 350, 627
FokI GGATG 2 cut(s) 74, 554
Fsp4HI GCNGC 4 cut(s) 177, 264, 350, 627
FspBI CTAG 2 cut(s) 204, 296
GluI GCNGC 4 cut(s) 177, 264, 350, 627
HaeIII GGCC 2 cut(s) 143, 223
HapII CCGG 1 cut(s) 91
Hin1II CATG 2 cut(s) 442, 626
HindIII AAGCTT 1 cut(s) 56
HpaII CCGG 1 cut(s) 91
HphI GGTGA 2 cut(s) 50, 314
Hpy166II GTNNAC 1 cut(s) 620
Hpy188I TCNGA 1 cut(s) 106
Hpy188III TCNNGA 6 cut(s) 62, 296, 432, 446, 479, 670
Hpy8I GTNNAC 1 cut(s) 620
HpyAV CCTTC 1 cut(s) 686
HpyCH4III ACNGT 3 cut(s) 44, 138, 395
HpyCH4IV ACGT 1 cut(s) 523
HpyCH4V TGCA 2 cut(s) 391, 576
HpyF10VI GCNNNNNNNGC 2 cut(s) 209, 355
HpySE526I ACGT 1 cut(s) 523
Hsp92II CATG 2 cut(s) 442, 626
KpnI GGTACC 1 cut(s) 399
Ksp22I TGATCA 1 cut(s) 64
Kzo9I GATC 4 cut(s) 64, 81, 406, 672
LpnPI CCDG 9 cut(s) 21, 75, 104, 210, 237, 366, 473, 565, 630
Lsp1109I GCAGC 4 cut(s) 163, 250, 361, 613
MaeI CTAG 2 cut(s) 204, 296
MaeII ACGT 1 cut(s) 523
MaeIII GTNAC 1 cut(s) 38
MalI GATC 4 cut(s) 66, 83, 408, 674
MboI GATC 4 cut(s) 64, 81, 406, 672
MboII GAAGA 2 cut(s) 76, 645
MflI RGATCY 1 cut(s) 81
MluCI AATT 1 cut(s) 484
MnlI CCTC 6 cut(s) 233, 246, 246, 381, 484, 529
MseI TTAA 3 cut(s) 29, 519, 605
MspI CCGG 1 cut(s) 91
MspR9I CCNGG 2 cut(s) 92, 225
MvaI CCWGG 1 cut(s) 225
MwoI GCNNNNNNNGC 2 cut(s) 209, 355
NciI CCSGG 1 cut(s) 92
NdeII GATC 4 cut(s) 64, 81, 406, 672
NheI GCTAGC 1 cut(s) 203
NlaIII CATG 2 cut(s) 442, 626
NlaIV GGNNCC 2 cut(s) 96, 397
NmuCI GTSAC 1 cut(s) 38
NspI RCATGY 1 cut(s) 626
PaeR7I CTCGAG 1 cut(s) 237
PflMI CCANNNNNTGG 1 cut(s) 380
PkrI GCNGC 4 cut(s) 178, 265, 351, 628
PshBI ATTAAT 1 cut(s) 605
Psp6I CCWGG 1 cut(s) 223
PspGI CCWGG 1 cut(s) 223
PspN4I GGNNCC 2 cut(s) 96, 397
PspPI GGNCC 2 cut(s) 141, 227
PspXI VCTCGAGB 1 cut(s) 237
PsuI RGATCY 1 cut(s) 81
RsaI GTAC 3 cut(s) 114, 397, 666
RsaNI GTAC 3 cut(s) 113, 396, 665
SaqAI TTAA 3 cut(s) 29, 519, 605
SatI GCNGC 4 cut(s) 177, 264, 350, 627
Sau3AI GATC 4 cut(s) 64, 81, 406, 672
Sau96I GGNCC 2 cut(s) 141, 227
ScrFI CCNGG 2 cut(s) 92, 225
Sfr274I CTCGAG 1 cut(s) 237
SinI GGWCC 1 cut(s) 227
SlaI CTCGAG 1 cut(s) 237
SmlI CTYRAG 3 cut(s) 237, 353, 668
SmoI CTYRAG 3 cut(s) 237, 353, 668
Sse9I AATT 1 cut(s) 484
SspMI CTAG 2 cut(s) 204, 296
StyD4I CCNGG 2 cut(s) 90, 223
TaaI ACNGT 3 cut(s) 44, 138, 395
TaiI ACGT 1 cut(s) 526
TaqI TCGA 3 cut(s) 238, 431, 480
TasI AATT 1 cut(s) 484
Tru1I TTAA 3 cut(s) 29, 519, 605
Tru9I TTAA 3 cut(s) 29, 519, 605
TscAI CASTG 1 cut(s) 143
TseFI GTSAC 1 cut(s) 38
TseI GCWGC 4 cut(s) 176, 263, 349, 626
Tsp45I GTSAC 1 cut(s) 38
TspDTI ATGAA 3 cut(s) 36, 76, 351
TspGWI ACGGA 2 cut(s) 130, 602
TspRI CASTG 1 cut(s) 143
Van91I CCANNNNNTGG 1 cut(s) 380
VpaK11BI GGWCC 1 cut(s) 227
VspI ATTAAT 1 cut(s) 605
XbaI TCTAGA 1 cut(s) 295
XceI RCATGY 1 cut(s) 626
XhoI CTCGAG 1 cut(s) 237
XspI CTAG 2 cut(s) 204, 296
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.