pycom09g12390

WD repeat-containing protein

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
pyrus_communis
Chr9
OG0001993
Physical Location & Seq
Reverse (-)
10919854 .. 10920592
739 bp
Loading structure...
UTR
Exon/CDS
Intron
N/A

Sequence Viewer

Length: 618 bp
ATGGGTGCTATAACATCACTGGCCATCAATGTATCAGAAGACATCCTATACTCAGCTTCCATTGACAGAACAGTCAAAGTATGGAGAATTTCAGACCTAAAATCTGATGATGGCGTGCTCTACACGGCCTCAGACGATGCCACAGTCCGAGTGTGGCGCCGTAACTTCTGGCGTGGAGACCAGCTTCCTCATTCACTCACTGTAACTTTACCCACCAAGAATTCCCCCGTCAAAACCCTAACCCTAACCCCAGATAGCGGAGTTTTGTACGGCGGGTGCACTGATGGGTACATACACTATTGGCTCAAGGGTTGGTTCTCTGGCCAATTACAGTATGGGGGTGCACTCCAAGGTCACACCCATGCAGTGATGTGCTTGGCCAGCGTGGCCAACTACGTGGTCAGTGGGTCAGCAGACTCCACAAGTAGGATTTGGTCTAGAGATGTGGAAGATGGTCAGCACACTTGCCTGGCAGTGCTCGTAGGGCATAGAGGGCCGATCAGATGTGTCACCGCGTTTTCCGGAAGATTAGGGGATGAGGCCGAGGAGGGTTGCACGATTTGTACTGGAAGTCTTGACGGTGTTCTTAAAGTGTGGCGTGTAGTCGAAGAGAGGTGA
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
No pathways identified.
Pfam Domains
Protein Families
WD40

Protein Analysis

206

Amino Acids

22.37

Weight (kDa)

6.04

Isoelectric Point (pI)

32.39

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
Beta-prop_WDR3_2nd PF25172 32 - 150 1.4e-08 WDR3 second beta-propeller domain
Beta-prop_WDR5 PF25175 41 - 201 1.5e-15 WDR5 beta-propeller domain
Beta-prop_WDR3_1st PF25173 66 - 201 1.7e-12 WDR3 first beta-propeller domain
Beta-prop_THOC3 PF25174 73 - 201 1.7e-07 THOC3 beta-propeller domain
Beta-prop_TEP1_2nd PF25047 87 - 202 4.7e-09 TEP-1 second beta-propeller
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

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Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AasI GACNNNNNNGTC 1 cut(s) 71
AccB1I GGYRCC 1 cut(s) 156
AccII CGCG 1 cut(s) 515
AccIII TCCGGA 1 cut(s) 521
AciI CCGC 3 cut(s) 258, 273, 513
AcoI YGGCCR 4 cut(s) 21, 322, 378, 387
AcsI RAATTY 2 cut(s) 87, 220
AcyI GRCGYC 1 cut(s) 157
AfaI GTAC 3 cut(s) 269, 290, 565
AfiI CCNNNNNNNGG 2 cut(s) 257, 426
AjnI CCWGG 1 cut(s) 468
AluBI AGCT 2 cut(s) 56, 184
AluI AGCT 2 cut(s) 56, 184
Alw21I GWGCWC 4 cut(s) 120, 281, 346, 480
Alw26I GTCTC 1 cut(s) 171
Alw44I GTGCAC 2 cut(s) 277, 342
Aor13HI TCCGGA 1 cut(s) 521
AoxI GGCC 7 cut(s) 21, 126, 322, 378, 387, 494, 540
ApaLI GTGCAC 2 cut(s) 277, 342
ApoI RAATTY 2 cut(s) 87, 220
AspLEI GCGC 1 cut(s) 159
AspS9I GGNCC 1 cut(s) 494
AsuHPI GGTGA 1 cut(s) 502
BaeGI GKGCMC 2 cut(s) 281, 346
BalI TGGCCA 4 cut(s) 23, 324, 380, 389
BanI GGYRCC 1 cut(s) 156
BbsI GAAGAC 1 cut(s) 45
Bbv12I GWGCWC 4 cut(s) 120, 281, 346, 480
BccI CCATC 4 cut(s) 32, 104, 278, 446
BceAI ACGGC 3 cut(s) 141, 144, 286
BciT130I CCWGG 1 cut(s) 470
BcoDI GTCTC 1 cut(s) 171
BfaI CTAG 1 cut(s) 438
BfoI RGCGCY 1 cut(s) 160
BglI GCCNNNNNGGC 1 cut(s) 386
Bme1390I CCNGG 1 cut(s) 470
BmgT120I GGNCC 1 cut(s) 494
BmiI GGNNCC 1 cut(s) 158
BmrFI CCNGG 1 cut(s) 470
BmsI GCATC 1 cut(s) 127
BpiI GAAGAC 1 cut(s) 45
BpuEI CTTGAG 1 cut(s) 290
BsaAI YACGTR 1 cut(s) 397
BsaHI GRCGYC 1 cut(s) 157
BsaI GGTCTC 1 cut(s) 171
BsaJI CCNNGG 2 cut(s) 349, 543
BsaWI WCCGGW 1 cut(s) 521
Bsc4I CCNNNNNNNGG 2 cut(s) 257, 426
Bse1I ACTGG 2 cut(s) 24, 571
BseAI TCCGGA 1 cut(s) 521
BseBI CCWGG 1 cut(s) 470
BseDI CCNNGG 2 cut(s) 349, 543
BseGI GGATG 2 cut(s) 42, 541
BseLI CCNNNNNNNGG 2 cut(s) 257, 426
BseMII CTCAG 2 cut(s) 66, 144
BseNI ACTGG 2 cut(s) 24, 571
BseRI GAGGAG 1 cut(s) 560
BseSI GKGCMC 2 cut(s) 281, 346
Bsh1236I CGCG 1 cut(s) 515
BshFI GGCC 7 cut(s) 23, 128, 324, 380, 389, 496, 542
BshNI GGYRCC 1 cut(s) 156
BsiHKAI GWGCWC 4 cut(s) 120, 281, 346, 480
BsiSI CCGG 1 cut(s) 522
BslI CCNNNNNNNGG 2 cut(s) 257, 426
BsmAI GTCTC 1 cut(s) 171
BsnI GGCC 7 cut(s) 23, 128, 324, 380, 389, 496, 542
Bso31I GGTCTC 1 cut(s) 171
Bsp1286I GDGCHC 4 cut(s) 120, 281, 346, 480
Bsp13I TCCGGA 1 cut(s) 521
Bsp143I GATC 1 cut(s) 498
BspACI CCGC 3 cut(s) 258, 273, 513
BspANI GGCC 7 cut(s) 23, 128, 324, 380, 389, 496, 542
BspCNI CTCAG 2 cut(s) 65, 143
BspEI TCCGGA 1 cut(s) 521
BspFNI CGCG 1 cut(s) 515
BspLI GGNNCC 1 cut(s) 158
BspT107I GGYRCC 1 cut(s) 156
BspTNI GGTCTC 1 cut(s) 171
BsrI ACTGG 2 cut(s) 24, 571
BssECI CCNNGG 2 cut(s) 349, 543
BssMI GATC 1 cut(s) 498
BssNI GRCGYC 1 cut(s) 157
BssT1I CCWWGG 1 cut(s) 349
Bst2UI CCWGG 1 cut(s) 470
Bst4CI ACNGT 5 cut(s) 73, 145, 202, 333, 581
Bst6I CTCTTC 1 cut(s) 603
BstACI GRCGYC 1 cut(s) 157
BstBAI YACGTR 1 cut(s) 397
BstC8I GCNNGC 2 cut(s) 116, 382
BstDEI CTNAG 2 cut(s) 52, 130
BstF5I GGATG 2 cut(s) 42, 541
BstFNI CGCG 1 cut(s) 515
BstH2I RGCGCY 1 cut(s) 160
BstHHI GCGC 1 cut(s) 159
BstKTI GATC 1 cut(s) 501
BstMAI GTCTC 1 cut(s) 171
BstMBI GATC 1 cut(s) 498
BstMWI GCNNNNNNNGC 4 cut(s) 381, 386, 484, 493
BstNI CCWGG 1 cut(s) 470
BstSCI CCNGG 1 cut(s) 468
BstSLI GKGCMC 2 cut(s) 281, 346
BstUI CGCG 1 cut(s) 515
BstV2I GAAGAC 1 cut(s) 45
BstXI CCANNNNNNTGG 1 cut(s) 397
BsuRI GGCC 7 cut(s) 23, 128, 324, 380, 389, 496, 542
BtsCI GGATG 2 cut(s) 42, 541
BtsI GCAGTG 2 cut(s) 372, 480
BtsIMutI CAGTG 6 cut(s) 17, 198, 279, 372, 409, 480
Cac8I GCNNGC 2 cut(s) 116, 382
CfoI GCGC 1 cut(s) 159
Cfr13I GGNCC 1 cut(s) 494
Csp6I GTAC 3 cut(s) 268, 289, 564
CviAII CATG 1 cut(s) 362
CviQI GTAC 3 cut(s) 268, 289, 564
DdeI CTNAG 2 cut(s) 52, 130
DinI GGCGCC 1 cut(s) 158
DpnI GATC 1 cut(s) 500
DpnII GATC 1 cut(s) 498
DrdI GACNNNNNNGTC 1 cut(s) 71
DseDI GACNNNNNNGTC 1 cut(s) 71
EaeI YGGCCR 4 cut(s) 21, 322, 378, 387
Eam1104I CTCTTC 1 cut(s) 603
EarI CTCTTC 1 cut(s) 603
Eco130I CCWWGG 1 cut(s) 349
Eco31I GGTCTC 1 cut(s) 171
EcoRI GAATTC 1 cut(s) 220
EcoRII CCWGG 1 cut(s) 468
EcoT14I CCWWGG 1 cut(s) 349
EgeI GGCGCC 1 cut(s) 158
EheI GGCGCC 1 cut(s) 158
ErhI CCWWGG 1 cut(s) 349
FaeI CATG 1 cut(s) 365
FaiI YATR 7 cut(s) 11, 49, 82, 293, 336, 363, 489
FatI CATG 1 cut(s) 361
FauI CCCGC 1 cut(s) 266
FokI GGATG 2 cut(s) 29, 548
FspBI CTAG 1 cut(s) 438
GlaI GCGC 1 cut(s) 158
HaeII RGCGCY 1 cut(s) 160
HaeIII GGCC 7 cut(s) 23, 128, 324, 380, 389, 496, 542
HapII CCGG 1 cut(s) 522
HhaI GCGC 1 cut(s) 159
Hin1I GRCGYC 1 cut(s) 157
Hin1II CATG 1 cut(s) 365
Hin6I GCGC 1 cut(s) 157
HinP1I GCGC 1 cut(s) 157
HinfI GANTC 1 cut(s) 416
HpaII CCGG 1 cut(s) 522
HphI GGTGA 1 cut(s) 502
Hpy166II GTNNAC 2 cut(s) 279, 344
Hpy188I TCNGA 6 cut(s) 37, 94, 106, 133, 149, 503
Hpy188III TCNNGA 3 cut(s) 438, 522, 575
Hpy8I GTNNAC 2 cut(s) 279, 344
HpyCH4III ACNGT 5 cut(s) 73, 145, 202, 333, 581
HpyCH4IV ACGT 1 cut(s) 396
HpyCH4V TGCA 4 cut(s) 279, 344, 365, 555
HpyF10VI GCNNNNNNNGC 4 cut(s) 381, 386, 484, 493
HpyF3I CTNAG 2 cut(s) 52, 130
HpySE526I ACGT 1 cut(s) 396
Hsp92I GRCGYC 1 cut(s) 157
Hsp92II CATG 1 cut(s) 365
HspAI GCGC 1 cut(s) 157
KasI GGCGCC 1 cut(s) 156
Kpn2I TCCGGA 1 cut(s) 521
Kzo9I GATC 1 cut(s) 498
LweI GCATC 1 cut(s) 127
MaeI CTAG 1 cut(s) 438
MaeII ACGT 1 cut(s) 396
MaeIII GTNAC 4 cut(s) 161, 202, 353, 508
MalI GATC 1 cut(s) 500
MboI GATC 1 cut(s) 498
MboII GAAGA 3 cut(s) 50, 461, 537
MhlI GDGCHC 4 cut(s) 120, 281, 346, 480
MlsI TGGCCA 4 cut(s) 23, 324, 380, 389
MluCI AATT 3 cut(s) 87, 220, 326
MluNI TGGCCA 4 cut(s) 23, 324, 380, 389
Mly113I GGCGCC 1 cut(s) 157
MlyI GAGTC 1 cut(s) 410
MnlI CCTC 7 cut(s) 139, 198, 485, 532, 538, 541, 606
Mox20I TGGCCA 4 cut(s) 23, 324, 380, 389
MroI TCCGGA 1 cut(s) 521
MscI TGGCCA 4 cut(s) 23, 324, 380, 389
MseI TTAA 1 cut(s) 588
MslI CAYNNNNRTG 1 cut(s) 360
Msp20I TGGCCA 4 cut(s) 23, 324, 380, 389
MspI CCGG 1 cut(s) 522
MspR9I CCNGG 1 cut(s) 470
MvaI CCWGG 1 cut(s) 470
MvnI CGCG 1 cut(s) 515
MwoI GCNNNNNNNGC 4 cut(s) 381, 386, 484, 493
NarI GGCGCC 1 cut(s) 157
NdeII GATC 1 cut(s) 498
NlaIII CATG 1 cut(s) 365
NlaIV GGNNCC 1 cut(s) 158
NmeAIII GCCGAG 1 cut(s) 568
NmuCI GTSAC 2 cut(s) 353, 508
PleI GAGTC 1 cut(s) 410
PluTI GGCGCC 1 cut(s) 160
PpsI GAGTC 1 cut(s) 410
Ppu21I YACGTR 1 cut(s) 397
Psp6I CCWGG 1 cut(s) 468
PspGI CCWGG 1 cut(s) 468
PspN4I GGNNCC 1 cut(s) 158
PspPI GGNCC 1 cut(s) 494
RsaI GTAC 3 cut(s) 269, 290, 565
RsaNI GTAC 3 cut(s) 268, 289, 564
RseI CAYNNNNRTG 1 cut(s) 360
SaqAI TTAA 1 cut(s) 588
Sau3AI GATC 1 cut(s) 498
Sau96I GGNCC 1 cut(s) 494
SchI GAGTC 1 cut(s) 410
ScrFI CCNGG 1 cut(s) 470
SduI GDGCHC 4 cut(s) 120, 281, 346, 480
SetI ASST 6 cut(s) 58, 99, 186, 355, 399, 617
SfaNI GCATC 1 cut(s) 127
SfiI GGCCNNNNNGGCC 1 cut(s) 386
SfoI GGCGCC 1 cut(s) 158
SmiMI CAYNNNNRTG 1 cut(s) 360
SmlI CTYRAG 1 cut(s) 305
SmoI CTYRAG 1 cut(s) 305
Sse9I AATT 3 cut(s) 87, 220, 326
SsiI CCGC 3 cut(s) 258, 273, 513
SspDI GGCGCC 1 cut(s) 156
SspMI CTAG 1 cut(s) 438
StyD4I CCNGG 1 cut(s) 468
StyI CCWWGG 1 cut(s) 349
TaaI ACNGT 5 cut(s) 73, 145, 202, 333, 581
TaiI ACGT 1 cut(s) 399
TaqI TCGA 1 cut(s) 606
TasI AATT 3 cut(s) 87, 220, 326
TatI WGTACW 1 cut(s) 563
Tru1I TTAA 1 cut(s) 588
Tru9I TTAA 1 cut(s) 588
TscAI CASTG 6 cut(s) 24, 205, 286, 372, 409, 480
TseFI GTSAC 2 cut(s) 353, 508
Tsp45I GTSAC 2 cut(s) 353, 508
TspRI CASTG 6 cut(s) 24, 205, 286, 372, 409, 480
VneI GTGCAC 2 cut(s) 277, 342
XapI RAATTY 2 cut(s) 87, 220
XbaI TCTAGA 1 cut(s) 437
XcmI CCANNNNNNNNNTGG 1 cut(s) 332
XspI CTAG 1 cut(s) 438
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.