pycom09g16440

Belongs to the ubiquitin-conjugating enzyme family

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
pyrus_communis
Chr9
OG0006585
Physical Location & Seq
Forward (+)
16730059 .. 16733793
3735 bp
Loading structure...
UTR
Exon/CDS
Intron
N/A

Sequence Viewer

Length: 1398 bp
ATGGCCTCTTCCGCTTCTGGAGAAGCCCTCAATCCCCCCGTCTCCTTGAAACTCAAGCAGAAAGAGGGTGCTTTGGACAGTGATGCTAAGTACTCAGCTAGTGTAGTCGGATCAGAAGATGACACTGATCTCTTAAAGAGTTCAACATCAGGATCAACCAATGCAAATGTTGTCAAAACTTCCAATTTTTTTTCATCTAATGATGATGATATGAGCATGGACGATGAAGACAATGATGATGACGATGATGATAATGACTATGATTGTGATGACAACGATGGTTTTCTGTATGATGACGGTGATGATTGCATGACTCTGCAATCTCAGTTTGATAATGTGGACTTGCCTCCTGGTGTCGAAGCAACTCTTCCCTGGTTGAATGATCGATCCTCATCACCGAGTGAGAAGCCATCCACTAAGACGCTGGCTATTTCAGATCTACCACCATGTAGTTCAACTGTTCCTTCTGAAGCAAGTTCAACTGCTCCTTTTGAGGCAAGTTCTAGTAAGGAACCGGCAGACAGCAATGATGACAGTATCCTGCCAAAGTCTCAACAGTTTAAGAAATTTGATATGGTGGAAGATTTCTCGGATCACCACTTTAGCCGCATGGGATTTTCAGATGAGCAGCCACCCAGATATTGGGCAAAGCGAATTCAGGATGAATGGAAAATTTTGGAGAAGGACTTACCAGACACTATATTTGTCAGGGTTTATGAAGCAAGAATGGAACTTCTGAGGGGTGTCATTATAGGACCTCCGGGCACTCCCTACCATGACGGTCTTTTTGTCTTTGATTGTCTCTTCCCAACTAACTATCCTAAATCACCACCGATGGTGTACTACTATTCAGGTGGTCTTCGATTGAATCCAAATTTGTACGAGTGTGGGAAAGTTTGTCTGAGTCTCCTAGGCACTTGGAGTGGCAAACAAAATGAAAATTGGGTCCCAGGGCAATCAACAATGCTACAAGTTTTAGTCTCTATACAAGCTCTTATTTTGAATGCAGAGCCCTTCTTCAATGAACCTGGGCATGAGTCATCGTATGTTGGAGCAGAAGGGCAAAGGCAATCCAGAAAGTACAATGAGGATACCTTTATTCTATCTCTAAAAACGATGATTTATACACTAAGGAGGCCGCCTAAGTATTTTGAGGACTTTGTCTTGGGTCATTTTCGCGAACGTGCACACAATATTCTGGCAGCATGTAAAGCATATAGTGAGGGCACTCTGGTGGGGTCTGATAGCACATCCCAGAATGCAGAAAAAGGTGGCTCCGCACAATTCAAGGCAAGTGTAGCTAAGATGATGAATACACTTGTTACACATTTCACCAGAAATGGATCAACCGACTGTGAGAAGTTTAGAGTTGAAGCCTCACATTTGACTAGCAGCTGA

Protein Analysis

466

Amino Acids

51.53

Weight (kDa)

4.53

Isoelectric Point (pI)

47.22

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
UQ_con PF00179 218 - 347 1.7e-21 Ubiquitin-conjugating enzyme
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

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Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AccB7I CCANNNNNTGG 1 cut(s) 642
AccII CGCG 1 cut(s) 1179
AciI CCGC 4 cut(s) 12, 607, 1139, 1278
AclWI GGATC 5 cut(s) 118, 160, 381, 600, 1351
AcsI RAATTY 4 cut(s) 566, 654, 672, 874
AcuI CTGAAG 1 cut(s) 489
AdeI CACNNNGTG 1 cut(s) 401
AfaI GTAC 4 cut(s) 92, 842, 881, 1082
AfiI CCNNNNNNNGG 1 cut(s) 642
AjnI CCWGG 4 cut(s) 349, 371, 949, 1027
AleI CACNNNNGTG 1 cut(s) 1232
AluBI AGCT 4 cut(s) 98, 992, 1301, 1395
AluI AGCT 4 cut(s) 98, 992, 1301, 1395
Alw21I GWGCWC 1 cut(s) 1189
Alw26I GTCTC 5 cut(s) 46, 555, 806, 911, 985
Alw44I GTGCAC 1 cut(s) 1185
AlwI GGATC 5 cut(s) 118, 160, 381, 600, 1351
AoxI GGCC 2 cut(s) 3, 1136
ApaLI GTGCAC 1 cut(s) 1185
ApeKI GCWGC 3 cut(s) 628, 1202, 1392
ApoI RAATTY 4 cut(s) 566, 654, 672, 874
ArsI GACNNNNNNTTYG 4 cut(s) 686, 718, 770, 802
AspA2I CCTAGG 1 cut(s) 910
AspS9I GGNCC 2 cut(s) 755, 946
AsuC2I CCSGG 1 cut(s) 762
AsuHPI GGTGA 5 cut(s) 311, 387, 587, 819, 1324
AvaII GGWCC 2 cut(s) 755, 946
AvrII CCTAGG 1 cut(s) 910
BaeGI GKGCMC 3 cut(s) 767, 1189, 1229
BanII GRGCYC 1 cut(s) 1014
BbsI GAAGAC 2 cut(s) 234, 851
Bbv12I GWGCWC 1 cut(s) 1189
BbvI GCAGC 2 cut(s) 640, 1214
BccI CCATC 3 cut(s) 272, 418, 829
BciT130I CCWGG 4 cut(s) 351, 373, 951, 1029
BciVI GTATCC 2 cut(s) 548, 1084
BcnI CCSGG 1 cut(s) 762
BcoDI GTCTC 5 cut(s) 46, 555, 806, 911, 985
BfaI CTAG 4 cut(s) 99, 504, 911, 1389
BfuI GTATCC 2 cut(s) 548, 1084
BglII AGATCT 1 cut(s) 436
BisI GCNGC 5 cut(s) 607, 629, 1139, 1203, 1393
BlnI CCTAGG 1 cut(s) 910
BlsI GCNGC 5 cut(s) 608, 630, 1140, 1204, 1394
BmcAI AGTACT 1 cut(s) 92
Bme1390I CCNGG 5 cut(s) 351, 373, 762, 951, 1029
Bme18I GGWCC 2 cut(s) 755, 946
BmgT120I GGNCC 2 cut(s) 755, 946
BmiI GGNNCC 4 cut(s) 513, 947, 948, 1276
BmrFI CCNGG 5 cut(s) 351, 373, 762, 951, 1029
BmsI GCATC 1 cut(s) 73
BpiI GAAGAC 2 cut(s) 234, 851
BplI GAGNNNNNCTC 2 cut(s) 12, 44
BpmI CTGGAG 1 cut(s) 39
BpuEI CTTGAG 1 cut(s) 38
BpuMI CCSGG 1 cut(s) 762
Bsa29I ATCGAT 1 cut(s) 385
BsaBI GATNNNNATC 1 cut(s) 391
BsaJI CCNNGG 5 cut(s) 371, 910, 949, 950, 1028
Bsc4I CCNNNNNNNGG 1 cut(s) 642
Bse118I RCCGGY 1 cut(s) 514
Bse3DI GCAATG 1 cut(s) 532
Bse8I GATNNNNATC 1 cut(s) 391
BseBI CCWGG 4 cut(s) 351, 373, 951, 1029
BseCI ATCGAT 1 cut(s) 385
BseDI CCNNGG 5 cut(s) 371, 910, 949, 950, 1028
BseGI GGATG 3 cut(s) 410, 667, 1250
BseJI GATNNNNATC 1 cut(s) 391
BseLI CCNNNNNNNGG 1 cut(s) 642
BseMI GCAATG 1 cut(s) 532
BseMII CTCAG 4 cut(s) 108, 338, 728, 893
BseSI GKGCMC 3 cut(s) 767, 1189, 1229
BseXI GCAGC 2 cut(s) 640, 1214
Bsh1236I CGCG 1 cut(s) 1179
BshFI GGCC 2 cut(s) 5, 1138
BshVI ATCGAT 1 cut(s) 385
BsiHKAI GWGCWC 1 cut(s) 1189
BsiSI CCGG 2 cut(s) 515, 761
BslFI GGGAC 1 cut(s) 932
BslI CCNNNNNNNGG 1 cut(s) 642
BsmAI GTCTC 5 cut(s) 46, 555, 806, 911, 985
BsmBI CGTCTC 1 cut(s) 46
BsmFI GGGAC 1 cut(s) 932
BsmI GAATGC 2 cut(s) 1009, 1264
BsnI GGCC 2 cut(s) 5, 1138
Bsp1286I GDGCHC 4 cut(s) 767, 1014, 1189, 1229
Bsp143I GATC 8 cut(s) 110, 127, 152, 382, 386, 436, 592, 1343
Bsp68I TCGCGA 1 cut(s) 1179
BspACI CCGC 4 cut(s) 12, 607, 1139, 1278
BspANI GGCC 2 cut(s) 5, 1138
BspCNI CTCAG 4 cut(s) 107, 337, 729, 894
BspDI ATCGAT 1 cut(s) 385
BspFNI CGCG 1 cut(s) 1179
BspLI GGNNCC 4 cut(s) 513, 947, 948, 1276
BspPI GGATC 5 cut(s) 118, 160, 381, 600, 1351
BsrDI GCAATG 1 cut(s) 532
BsrFI RCCGGY 1 cut(s) 514
BssAI RCCGGY 1 cut(s) 514
BssECI CCNNGG 5 cut(s) 371, 910, 949, 950, 1028
BssMI GATC 8 cut(s) 110, 127, 152, 382, 386, 436, 592, 1343
BssT1I CCWWGG 1 cut(s) 910
Bst2UI CCWGG 4 cut(s) 351, 373, 951, 1029
Bst4CI ACNGT 7 cut(s) 80, 299, 460, 536, 558, 782, 1355
Bst6I CTCTTC 3 cut(s) 13, 372, 809
BstC8I GCNNGC 1 cut(s) 426
BstDEI CTNAG 9 cut(s) 87, 94, 324, 417, 737, 902, 1130, 1143, 1302
BstF5I GGATG 3 cut(s) 410, 667, 1250
BstFNI CGCG 1 cut(s) 1179
BstKTI GATC 8 cut(s) 113, 130, 155, 385, 389, 439, 595, 1346
BstMAI GTCTC 5 cut(s) 46, 555, 806, 911, 985
BstMBI GATC 8 cut(s) 110, 127, 152, 382, 386, 436, 592, 1343
BstMWI GCNNNNNNNGC 3 cut(s) 11, 1211, 1298
BstNI CCWGG 4 cut(s) 351, 373, 951, 1029
BstNSI RCATGY 1 cut(s) 1209
BstSCI CCNGG 5 cut(s) 349, 371, 760, 949, 1027
BstSLI GKGCMC 3 cut(s) 767, 1189, 1229
BstUI CGCG 1 cut(s) 1179
BstV1I GCAGC 2 cut(s) 640, 1214
BstV2I GAAGAC 2 cut(s) 234, 851
BstX2I RGATCY 1 cut(s) 436
BstYI RGATCY 1 cut(s) 436
Bsu15I ATCGAT 1 cut(s) 385
BsuI GTATCC 2 cut(s) 548, 1084
BsuRI GGCC 2 cut(s) 5, 1138
BsuTUI ATCGAT 1 cut(s) 385
BtsCI GGATG 3 cut(s) 410, 667, 1250
BtsIMutI CAGTG 2 cut(s) 85, 123
BtuMI TCGCGA 1 cut(s) 1179
Cac8I GCNNGC 1 cut(s) 426
Cfr10I RCCGGY 1 cut(s) 514
Cfr13I GGNCC 2 cut(s) 755, 946
ClaI ATCGAT 1 cut(s) 385
CseI GACGC 1 cut(s) 430
Csp6I GTAC 4 cut(s) 91, 841, 880, 1081
CviAII CATG 7 cut(s) 217, 310, 447, 610, 776, 1034, 1206
CviQI GTAC 4 cut(s) 91, 841, 880, 1081
DdeI CTNAG 9 cut(s) 87, 94, 324, 417, 737, 902, 1130, 1143, 1302
DpnI GATC 8 cut(s) 112, 129, 154, 384, 388, 438, 594, 1345
DpnII GATC 8 cut(s) 110, 127, 152, 382, 386, 436, 592, 1343
DraIII CACNNNGTG 1 cut(s) 401
Eam1104I CTCTTC 3 cut(s) 13, 372, 809
EarI CTCTTC 3 cut(s) 13, 372, 809
Eco130I CCWWGG 1 cut(s) 910
Eco24I GRGCYC 1 cut(s) 1014
Eco47I GGWCC 2 cut(s) 755, 946
Eco57I CTGAAG 1 cut(s) 489
EcoO109I RGGNCCY 2 cut(s) 755, 946
EcoRI GAATTC 1 cut(s) 654
EcoRII CCWGG 4 cut(s) 349, 371, 949, 1027
EcoT14I CCWWGG 1 cut(s) 910
EcoT38I GRGCYC 1 cut(s) 1014
ErhI CCWWGG 1 cut(s) 910
Esp3I CGTCTC 1 cut(s) 46
FaeI CATG 7 cut(s) 220, 313, 450, 613, 779, 1037, 1209
FalI AAGNNNNNCTT 2 cut(s) 351, 383
FaqI GGGAC 1 cut(s) 932
FatI CATG 7 cut(s) 216, 309, 446, 609, 775, 1033, 1205
Fnu4HI GCNGC 5 cut(s) 607, 629, 1139, 1203, 1393
FokI GGATG 3 cut(s) 397, 674, 1237
FriOI GRGCYC 1 cut(s) 1014
Fsp4HI GCNGC 5 cut(s) 607, 629, 1139, 1203, 1393
FspBI CTAG 4 cut(s) 99, 504, 911, 1389
GluI GCNGC 5 cut(s) 607, 629, 1139, 1203, 1393
GsuI CTGGAG 1 cut(s) 39
HaeIII GGCC 2 cut(s) 5, 1138
HapII CCGG 2 cut(s) 515, 761
HgaI GACGC 1 cut(s) 430
Hin1II CATG 7 cut(s) 220, 313, 450, 613, 779, 1037, 1209
HinfI GANTC 4 cut(s) 313, 868, 904, 1037
HpaII CCGG 2 cut(s) 515, 761
HphI GGTGA 5 cut(s) 311, 387, 587, 819, 1324
Hpy166II GTNNAC 3 cut(s) 340, 841, 1187
Hpy188I TCNGA 9 cut(s) 110, 115, 436, 469, 592, 622, 738, 903, 1243
Hpy188III TCNNGA 5 cut(s) 18, 150, 659, 1074, 1178
Hpy8I GTNNAC 3 cut(s) 340, 841, 1187
HpyAV CCTTC 4 cut(s) 474, 676, 1024, 1052
HpyCH4III ACNGT 7 cut(s) 80, 299, 460, 536, 558, 782, 1355
HpyCH4IV ACGT 1 cut(s) 1183
HpyCH4V TGCA 6 cut(s) 164, 309, 319, 1007, 1187, 1262
HpyF10VI GCNNNNNNNGC 3 cut(s) 11, 1211, 1298
HpyF3I CTNAG 9 cut(s) 87, 94, 324, 417, 737, 902, 1130, 1143, 1302
HpySE526I ACGT 1 cut(s) 1183
Hsp92II CATG 7 cut(s) 220, 313, 450, 613, 779, 1037, 1209
KflI GGGWCCC 1 cut(s) 946
Kzo9I GATC 8 cut(s) 110, 127, 152, 382, 386, 436, 592, 1343
LmnI GCTCC 3 cut(s) 490, 1052, 1280
Lsp1109I GCAGC 2 cut(s) 640, 1214
LweI GCATC 1 cut(s) 73
MaeI CTAG 4 cut(s) 99, 504, 911, 1389
MaeII ACGT 1 cut(s) 1183
MaeIII GTNAC 1 cut(s) 1321
MalI GATC 8 cut(s) 112, 129, 154, 384, 388, 438, 594, 1345
MboI GATC 8 cut(s) 110, 127, 152, 382, 386, 436, 592, 1343
MboII GAAGA 7 cut(s) 128, 239, 359, 593, 796, 851, 1009
MflI RGATCY 1 cut(s) 436
MhlI GDGCHC 4 cut(s) 767, 1014, 1189, 1229
MluCI AATT 7 cut(s) 184, 566, 654, 672, 874, 940, 1283
MlyI GAGTC 3 cut(s) 307, 913, 1046
MmeI TCCRAC 2 cut(s) 88, 1030
MseI TTAA 2 cut(s) 134, 561
MslI CAYNNNNRTG 1 cut(s) 1232
MspA1I CMGCKG 1 cut(s) 1395
MspI CCGG 2 cut(s) 515, 761
MspR9I CCNGG 5 cut(s) 351, 373, 762, 951, 1029
Mva1269I GAATGC 2 cut(s) 1009, 1264
MvaI CCWGG 4 cut(s) 351, 373, 951, 1029
MvnI CGCG 1 cut(s) 1179
MwoI GCNNNNNNNGC 3 cut(s) 11, 1211, 1298
NciI CCSGG 1 cut(s) 762
NdeII GATC 8 cut(s) 110, 127, 152, 382, 386, 436, 592, 1343
NlaIII CATG 7 cut(s) 220, 313, 450, 613, 779, 1037, 1209
NlaIV GGNNCC 4 cut(s) 513, 947, 948, 1276
NruI TCGCGA 1 cut(s) 1179
NspI RCATGY 1 cut(s) 1209
OliI CACNNNNGTG 1 cut(s) 1232
PasI CCCWGGG 1 cut(s) 950
PctI GAATGC 2 cut(s) 1009, 1264
PfeI GAWTC 1 cut(s) 868
PflFI GACNNNGTC 1 cut(s) 1160
PflMI CCANNNNNTGG 1 cut(s) 642
PkrI GCNGC 5 cut(s) 608, 630, 1140, 1204, 1394
PleI GAGTC 3 cut(s) 307, 912, 1045
PpsI GAGTC 3 cut(s) 307, 912, 1045
PpuMI RGGWCCY 2 cut(s) 755, 946
Psp5II RGGWCCY 2 cut(s) 755, 946
Psp6I CCWGG 4 cut(s) 349, 371, 949, 1027
PspGI CCWGG 4 cut(s) 349, 371, 949, 1027
PspN4I GGNNCC 4 cut(s) 513, 947, 948, 1276
PspPI GGNCC 2 cut(s) 755, 946
PspPPI RGGWCCY 2 cut(s) 755, 946
PsuI RGATCY 1 cut(s) 436
PsyI GACNNNGTC 1 cut(s) 1160
PvuII CAGCTG 1 cut(s) 1395
RruI TCGCGA 1 cut(s) 1179
RsaI GTAC 4 cut(s) 92, 842, 881, 1082
RsaNI GTAC 4 cut(s) 91, 841, 880, 1081
RseI CAYNNNNRTG 1 cut(s) 1232
SaqAI TTAA 2 cut(s) 134, 561
SatI GCNGC 5 cut(s) 607, 629, 1139, 1203, 1393
Sau3AI GATC 8 cut(s) 110, 127, 152, 382, 386, 436, 592, 1343
Sau96I GGNCC 2 cut(s) 755, 946
ScaI AGTACT 1 cut(s) 92
SchI GAGTC 3 cut(s) 307, 913, 1046
ScrFI CCNGG 5 cut(s) 351, 373, 762, 951, 1029
SduI GDGCHC 4 cut(s) 767, 1014, 1189, 1229
SfaNI GCATC 1 cut(s) 73
SinI GGWCC 2 cut(s) 755, 946
SmiMI CAYNNNNRTG 1 cut(s) 1232
SmlI CTYRAG 1 cut(s) 53
SmoI CTYRAG 1 cut(s) 53
Sse9I AATT 7 cut(s) 184, 566, 654, 672, 874, 940, 1283
SsiI CCGC 4 cut(s) 12, 607, 1139, 1278
SspI AATATT 1 cut(s) 1195
SspMI CTAG 4 cut(s) 99, 504, 911, 1389
StyD4I CCNGG 5 cut(s) 349, 371, 760, 949, 1027
StyI CCWWGG 1 cut(s) 910
TaaI ACNGT 7 cut(s) 80, 299, 460, 536, 558, 782, 1355
TaiI ACGT 1 cut(s) 1186
TaqI TCGA 3 cut(s) 357, 385, 862
TasI AATT 7 cut(s) 184, 566, 654, 672, 874, 940, 1283
TatI WGTACW 3 cut(s) 90, 840, 1080
TauI GCSGC 2 cut(s) 609, 1141
TfiI GAWTC 1 cut(s) 868
Tru1I TTAA 2 cut(s) 134, 561
Tru9I TTAA 2 cut(s) 134, 561
TscAI CASTG 2 cut(s) 85, 130
TseI GCWGC 3 cut(s) 628, 1202, 1392
TspDTI ATGAA 7 cut(s) 183, 240, 678, 732, 951, 1038, 1325
TspRI CASTG 2 cut(s) 85, 130
Tth111I GACNNNGTC 1 cut(s) 1160
Van91I CCANNNNNTGG 1 cut(s) 642
VneI GTGCAC 1 cut(s) 1185
VpaK11BI GGWCC 2 cut(s) 755, 946
XapI RAATTY 4 cut(s) 566, 654, 672, 874
XceI RCATGY 1 cut(s) 1209
XcmI CCANNNNNNNNNTGG 2 cut(s) 421, 639
XmaJI CCTAGG 1 cut(s) 910
XspI CTAG 4 cut(s) 99, 504, 911, 1389
ZrmI AGTACT 1 cut(s) 92
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.