Basic Information

Type: gene

Biological Identity

Species

pyrus_communis

Chromosome

Chr10

Orthogroup ID

OG0005132

Physical Location & Seq

Genomic Coordinates Forward (+)

3431988 .. 3438100

Gene Structure 6113 bp

Loading structure...

UTR

Exon/CDS

Intron

Transcript / Protein ID

N/A

Sequence Viewer

CDS Protein Promoter (2k)

Length: 3138 bp

ATGGAAAACAACCTGAGCGAAAGTAGTTGTGGCAGTGATGTGTATACAGTAAGAGATGTTGAGATTATACAGACAAGTGATGACCATGAAGCAAAAGCTAATGTCAATGCAAGCACTAGTTCACATAGTGATGGAGCTGAGAACTCAAATTCTTTAGAAGATGATACAAATGCTGAGATATGGGAACCCCCTGAACCTGATGATCCGGAGGATGACATGGAGGATAGTGTTGCTTTCAATGATGATGATGATGAGGAATGTGGTGATGGCATGAAATGGGGTACATCGAGTTCTTTGAGTCATTCTGGAGATGAAGGAAGTGGAAGTTATACGTTTAAAGAGGAAAAACAAAGAGCAATGGAAGCAGTGATTAATGGAAAGTTTAAAGCCTTGATCAGTCAGCTACTTAAATCTGTGGGTGTTGCATCTTTGGGGGAGGGTGGTGAAAGTTGGGTGGACATTATTGCTTCTTTGTCATGGGAAGCTGCTTCATTTTTGAAACCTGATGCTGTTGTTGGTAAAGCAATCGATCCAGATGGCTATGTGAAAGTAAAATGCATTGCAACTGGTGTTCGCACCCAAAGTCAACTAGTAAAAGGTTTGGTCTTCAAAAAGCATGCTGCACACAAGCATATGTCGACCAAGTACAAGAATCCAAGGTTGTTGCTAATCAAGGGAGTACTTGGTCAGTCTTCAAGTGGATTGTCATCCTTCAATTCAATGGAACAGGAAAGAGATTATCTGAAGTTTGTCATCGAAATGTTAGAATTGTGCCATCCAAATGTGGTTTTAGTGGAAAAAACTGTTTCTCGTGATATTCAAGAGGCTATTCTTGGGAATGGAATTACACTGATCTTTGACATGAAGCTCCATCGCCTAGAGAGAGTTGCTCGTTGTACCGGGTCACCAATTTTGTCATCAGATACAATGACGAGCAAAAATCTAAAACAGTGTGACTCTTTTCACATTGAAAAGTTTATAGAGGAGCATGCTGGAGGTGGAGGAAAGGTCCCAAGTAAAACATTAATGTTTATTGAGGGCTGTCCTACACGCCTAGGCTGTACGATTTTGCTGAAAGGAGCACGGAGTGATGAATTGAAGAAGGTCAAGTGTGTTGTGCAGTGTGCAGTTATTCTGGCGTATCATTTAATGCTCGAGACTGCTTTCCTTGTTGACCAGAGAGCAATGTTCTCTACACTTCCATTTGCTGATGTAGCAACAGATTTGTCCACTGATAAAGAAACCCTTAATTTAGGATCAATTAACTCATGTGTCCATCAGAATACAGAGACTAGTGCTGAAACTGGGTCTGATACAGTTGATATTCCCATTTCCAATGGATTTCATGAAGGATGTTCCCACAGTTCTACCTTGGAAGTCGAAGGCCGTTCCACATTTGATGAACCATACAATCCTGCGGTACTTTCTGGCTTGTCGTCCATTTCAGTATCTTTAAGGAAAGTTATAGGGGAGAATTTTCCCCTAGCATCTTCTTCTTATCAGTCTCTTTCTTCATACTTTGGATTCAGTGGAAGAGAACTGAATGACCCAATCACAAGATCTGTTTCTGTTTGTACAACTCCTGAGGAAACAGAGCATTGTGATGTTGAAGATAAAGGTAGTGTTGATGAAGAGAGATCACTTAATGGGAAAAATCAGACTTCATTTACATGCACAGAAGCTTCTCTTGAGGTGAAAGAAGATCATGGAAATAGTGAAGATCAAATGAAAAGTAAGAACCAAATCAGTGCTGTGTTGGATTCTCAGAGTATTTTGGTTTTGATGTCTAGCCAGAATGCATTAAGAGGCACTGTATGTGAGCAGAGCCGTTTTTCTCATATCATGTTCTACAAGAATTTCGATGTTCCCATTGGAAAGTTTCTTCAAGATAATTTACTCAATCAGCAAAGCCAGTGCACATCATGTGGTGGACTTCCAGAAGCTCACTTCTACTATTATGCACATCGTAACAAGCAGCTTACTATTCGAGTTAAACGGCTCCCTGGAGAAGTGCATTTGCCTGGTGGAGCAGAAGGGAAACTTTGGATGTGGAGTCGATGTGGTAAATGTAAATCTAGGAATGGAATCTCAAAGTGCACAAAAAGAGTATTGATTTCCACTTCTGCACGTGGTCTATCATTTGGAAATTTCCTGGAGCTCAATTTCTCGAACCCATCTTTGTCTAATTTATTTTCTAGCTGCGGCCATTCTTTGCAGGAGGACTTTCTCTACTTCTTTGGATTAGGCCCTATGGTTGCCTTGTTCAAGTATTCCCCTGTTACGACTTATACTGTTTCTGTGCCTCCTCTGAAGCTTCCATTCAGCAGTTCAATTAGACAAGACTGGCTTGTGAAAGAAACTCAGAATGTGTACATGAAATTGATTTGCTTGTTCACAGACGTTGCCAACTCTTTAGAAAAGATGAGATCTCAATTTGATGGGTTGGCTTTGAAACTTCAAGGCTCATTTAAAGAATTCTCTGACATTGAAGAGATGCTAAAGCAAGAAAAATCTGAGTTAGAGGTAGTGCTCAAGAATGCTGTTACCAGGACGGAGAACTCGGACCAGGTTGCCTACAAACTACTTAGCTTGAACAGAGTACTCTGGGAACTGCTCCTTGAAGCATGTATTTGGGAACGGCGCATGCAGTCATTACTGTCACCAGATCCTACGATGATCCATTCTAGAGAATCTGTGAAGGCAGAGGAAAAAGTTGATTCAGGTATTGGTACCAGTTTAGAAGTCAAATTGGATACAGCTGCAGAAGCTGATGGATCTCCAATAAAAGACATTCCAGTTGAAGGTCCTGTTCAAGAATCTAATGGAGCCGATCCAGTTAATGTGTCTGATTCGGCTGAGGGTTTCAAAACACCGAATGTGGATGGTTCAAGCCCAAAACGGTTGGCCAGGGAGGGGTCCAATCTGAGGAATGGCTCCAGCTATCATTGTGAGACTGATCAGCTAGCAGAAAATGTGGATGGTGCTTTTGCCGATTCAGATCTATCAATGAAAGGCACTTATCACCGTTCACTGTCATTCAACTTGGAAGGTTCACATGACTGGTTTTGGGCACCATTCTCCGAAATTCGTCAAGTTGGCATGAGAGATCTATCACAGAGTGTTCTTTCAAAAGTTTGA

Functional Annotation

Gene Ontology

Molecular Function
Biological Process
Cellular Component

GO:0000285 GO:0000322 GO:0000323 GO:0000324 GO:0000329 GO:0003674 GO:0003824 GO:0005488 GO:0005543 GO:0005575 GO:0005622 GO:0005623 GO:0005737 GO:0005768 GO:0005773 GO:0005774 GO:0005942 GO:0006629 GO:0006644 GO:0006650 GO:0006793 GO:0006796 GO:0006810 GO:0008150 GO:0008152 GO:0008289 GO:0009987 GO:0010008 GO:0012505 GO:0016020 GO:0016192 GO:0016197 GO:0016301 GO:0016307 GO:0016310 GO:0016482 GO:0016740 GO:0016772 GO:0016773 GO:0019637 GO:0019898 GO:0030258 GO:0031090 GO:0031410 GO:0031982 GO:0032266 GO:0032991 GO:0035032 GO:0035091 GO:0042147 GO:0043167 GO:0043168 GO:0043226 GO:0043227 GO:0043229 GO:0043231 GO:0044237 GO:0044238 GO:0044255 GO:0044422 GO:0044424 GO:0044425 GO:0044433 GO:0044437 GO:0044440 GO:0044444 GO:0044446 GO:0044464 GO:0046486 GO:0046488 GO:0046834 GO:0046854 GO:0046907 GO:0051179 GO:0051234 GO:0051641 GO:0051649 GO:0061695 GO:0070772 GO:0071704 GO:0097708 GO:0098588 GO:0098796 GO:0098805 GO:0098852 GO:1901981 GO:1902494 GO:1990234

KEGG Pathways

Metabolic & Signaling

ko00562 ko04070 ko04145 ko04810 map00562 map04070 map04145 map04810

Pfam Domains

Protein Families

Cpn60_TCP1 PIP5K

Protein Analysis

1046

Amino Acids

115.01

Weight (kDa)

5.09

Isoelectric Point (pI)

43.81

Instability Index

Protein Domains (Pfam)

Domain Name	Pfam ID	Position	E-value	Description
Cpn60_TCP1	PF00118	133 - 382	6.3e-22	TCP-1/cpn60 chaperonin family

Hydrophobicity Profile (Kyte-Doolittle)

AI Structure Prediction Report

Calculating structure properties...

Genomic Context

Gene Family Tree

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Align Labels (Cladogram)

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Orthologous Genes (Group: OG0005132)

Species	Orthologous Gene IDs
arabidopsis_thaliana	AT1G34260 AT1G34260 AT1G34260 AT1G34260
fragaria_vesca	FvH4_2g12440 FvH4_2g12440 FvH4_2g12440 FvH4_2g12440 FvH4_2g12440
malus_domestica	MD00G1078100.v1.1 MD10G1044000.v1.1 MD10G1044100.v1.1
prunus_persica	Prupe.8G050600_v2.0.a1
pyrus_communis	pycom05g03120 pycom05g03130 pycom10g03060 pycom10g03070 pycom10g03080
rosa_chinensis	RchiOBHm_Chr6g0272731
rosa_laevigata	RLG00000013661
rosa_multiflora	Rmu_co8449107.1_g000001 Rmu_sc0003080.1_g000001 Rmu_sc0014453.1_g000001
rosa_roxburghii	Rroxscaffold_7G00196050
rosa_rugosa	Rorug06G0070400
rosa_samantha	Rh6AG186600 Rh6BG189900 Rh6CG188700 Rh6DG180100
rosa_wichuraiana	Rw6G016100

Restriction Enzyme Sites

1 / 10

Enzyme	Recognition Site	Cut Count	Positions (bp)
Acc65I	GGTACC	1 cut(s)	2729
AccB1I	GGYRCC	2 cut(s)	2729, 3070
AccI	GTMKAC	2 cut(s)	44, 638
AccIII	TCCGGA	1 cut(s)	205
AciI	CCGC	2 cut(s)	1418, 2202
AclWI	GGATC	7 cut(s)	197, 524, 1264, 2660, 2671, 2782, 2825
AcoI	YGGCCR	2 cut(s)	2203, 2904
AcsI	RAATTY	6 cut(s)	148, 1474, 1855, 2146, 2474, 3084
AcuI	CTGAAG	2 cut(s)	764, 2330
AcvI	CACGTG	1 cut(s)	2129
AdeI	CACNNNGTG	3 cut(s)	128, 1088, 3119
AfiI	CCNNNNNNNGG	3 cut(s)	2801, 2913, 2925
AhlI	ACTAGT	3 cut(s)	116, 589, 1292
AjnI	CCWGG	6 cut(s)	2002, 2020, 2151, 2546, 2565, 2906
Alw21I	GWGCWC	5 cut(s)	1084, 1919, 2099, 2160, 2532
Alw26I	GTCTC	4 cut(s)	1151, 1283, 1509, 2945
Alw44I	GTGCAC	2 cut(s)	1915, 2095
AlwI	GGATC	7 cut(s)	197, 524, 1264, 2660, 2671, 2782, 2825
AlwNI	CAGNNNCTG	1 cut(s)	2768
Ama87I	CYCGRG	1 cut(s)	1154
Aor13HI	TCCGGA	1 cut(s)	205
AoxI	GGCC	4 cut(s)	1384, 2203, 2245, 2904
ApaLI	GTGCAC	2 cut(s)	1915, 2095
ApeKI	GCWGC	5 cut(s)	485, 620, 1975, 2199, 2759
ApoI	RAATTY	6 cut(s)	148, 1474, 1855, 2146, 2474, 3084
AseI	ATTAAT	2 cut(s)	372, 1025
Asp718I	GGTACC	1 cut(s)	2729
AspA2I	CCTAGG	1 cut(s)	1054
AspLEI	GCGC	1 cut(s)	2643
AspS9I	GGNCC	5 cut(s)	1009, 2246, 2563, 2804, 2916
AsuC2I	CCSGG	1 cut(s)	901
AsuHPI	GGTGA	6 cut(s)	275, 455, 897, 1705, 2652, 3014
AsuNHI	GCTAGC	1 cut(s)	2962
AvaI	CYCGRG	1 cut(s)	1154
AvaII	GGWCC	4 cut(s)	1009, 2563, 2804, 2916
AvrII	CCTAGG	1 cut(s)	1054
AxyI	CCTNAGG	1 cut(s)	1584
BaeGI	GKGCMC	3 cut(s)	1919, 2099, 3073
BalI	TGGCCA	1 cut(s)	2906
BanI	GGYRCC	2 cut(s)	2729, 3070
BanII	GRGCYC	1 cut(s)	2160
BauI	CACGAG	1 cut(s)	810
BbrPI	CACGTG	1 cut(s)	2129
BbsI	GAAGAC	2 cut(s)	598, 684
Bbv12I	GWGCWC	5 cut(s)	1084, 1919, 2099, 2160, 2532
BbvCI	CCTCAGC	1 cut(s)	2856
BbvI	GCAGC	5 cut(s)	472, 607, 1987, 2186, 2746
BceAI	ACGGC	4 cut(s)	1371, 1812, 2012, 2654
BciT130I	CCWGG	6 cut(s)	2004, 2022, 2153, 2548, 2567, 2908
BciVI	GTATCC	1 cut(s)	2746
BclI	TGATCA	2 cut(s)	393, 2956
BcnI	CCSGG	1 cut(s)	901
BcoDI	GTCTC	4 cut(s)	1151, 1283, 1509, 2945
BcuI	ACTAGT	3 cut(s)	116, 589, 1292
BfmI	CTRYAG	1 cut(s)	2760
BfuI	GTATCC	1 cut(s)	2746
BglII	AGATCT	4 cut(s)	1559, 2426, 2998, 3106
BisI	GCNGC	6 cut(s)	486, 621, 1976, 2200, 2203, 2760
BlnI	CCTAGG	1 cut(s)	1054
BlsI	GCNGC	6 cut(s)	487, 622, 1977, 2201, 2204, 2761
BmcAI	AGTACT	2 cut(s)	681, 2601
Bme1390I	CCNGG	7 cut(s)	901, 2004, 2022, 2153, 2548, 2567, 2908
Bme18I	GGWCC	4 cut(s)	1009, 2563, 2804, 2916
BmeT110I	CYCGRG	1 cut(s)	1154
BmgT120I	GGNCC	5 cut(s)	1009, 2246, 2563, 2804, 2916
BmiI	GGNNCC	8 cut(s)	186, 1011, 2000, 2731, 2827, 2917, 2935, 3072
BmrFI	CCNGG	7 cut(s)	901, 2004, 2022, 2153, 2548, 2567, 2908
BmrI	ACTGGG	1 cut(s)	1314
BmsI	GCATC	4 cut(s)	434, 496, 1496, 2484
BmtI	GCTAGC	1 cut(s)	2966
BmuI	ACTGGG	1 cut(s)	1314
BpiI	GAAGAC	2 cut(s)	598, 684
BplI	GAGNNNNNCTC	2 cut(s)	874, 906
BpmI	CTGGAG	5 cut(s)	327, 1014, 2025, 2174, 2920
Bpu10I	CCTNAGC	2 cut(s)	14, 2856
BpuEI	CTTGAG	2 cut(s)	1709, 2516
BpuMI	CCSGG	1 cut(s)	901
Bsa29I	ATCGAT	1 cut(s)	528
BsaAI	YACGTR	1 cut(s)	2129
BsaBI	GATNNNNATC	2 cut(s)	706, 2997
BsaJI	CCNNGG	5 cut(s)	656, 1054, 1371, 2002, 2907
BsaWI	WCCGGW	1 cut(s)	205
BsaXI	ACNNNNNCTCC	4 cut(s)	993, 1023, 2044, 2074
Bsc4I	CCNNNNNNNGG	3 cut(s)	2801, 2913, 2925
Bse1I	ACTGG	8 cut(s)	571, 1309, 1912, 2348, 2733, 2795, 2834, 3065
Bse21I	CCTNAGG	1 cut(s)	1584
Bse3DI	GCAATG	3 cut(s)	363, 558, 1191
Bse8I	GATNNNNATC	2 cut(s)	706, 2997
BseAI	TCCGGA	1 cut(s)	205
BseBI	CCWGG	6 cut(s)	2004, 2022, 2153, 2548, 2567, 2908
BseCI	ATCGAT	1 cut(s)	528
BseDI	CCNNGG	5 cut(s)	656, 1054, 1371, 2002, 2907
BseGI	GGATG	7 cut(s)	217, 707, 775, 1358, 2052, 2887, 2983
BseJI	GATNNNNATC	2 cut(s)	706, 2997
BseLI	CCNNNNNNNGG	3 cut(s)	2801, 2913, 2925
BseMI	GCAATG	3 cut(s)	363, 558, 1191
BseMII	CTCAG	9 cut(s)	5, 129, 165, 1575, 1778, 2375, 2505, 2847, 2915
BseNI	ACTGG	8 cut(s)	571, 1309, 1912, 2348, 2733, 2795, 2834, 3065
BseRI	GAGGAG	2 cut(s)	998, 2295
BseSI	GKGCMC	3 cut(s)	1919, 2099, 3073
BseXI	GCAGC	5 cut(s)	472, 607, 1987, 2186, 2746
BsgI	GTGCAG	4 cut(s)	606, 1139, 1146, 2109
BshFI	GGCC	4 cut(s)	1386, 2205, 2247, 2906
BshNI	GGYRCC	2 cut(s)	2729, 3070
BshVI	ATCGAT	1 cut(s)	528
BsiHKAI	GWGCWC	5 cut(s)	1084, 1919, 2099, 2160, 2532
BsiHKCI	CYCGRG	1 cut(s)	1154
BsiSI	CCGG	2 cut(s)	206, 900
BslFI	GGGAC	1 cut(s)	995
BslI	CCNNNNNNNGG	3 cut(s)	2801, 2913, 2925
BsmAI	GTCTC	4 cut(s)	1151, 1283, 1509, 2945
BsmFI	GGGAC	1 cut(s)	995
BsmI	GAATGC	2 cut(s)	1801, 2542
BsnI	GGCC	4 cut(s)	1386, 2205, 2247, 2906
BsoBI	CYCGRG	1 cut(s)	1154
Bsp1286I	GDGCHC	6 cut(s)	1084, 1919, 2099, 2160, 2532, 3073
Bsp13I	TCCGGA	1 cut(s)	205
Bsp1407I	TGTACA	2 cut(s)	1574, 2370
BspACI	CCGC	2 cut(s)	1418, 2202
BspANI	GGCC	4 cut(s)	1386, 2205, 2247, 2906
BspCNI	CTCAG	9 cut(s)	6, 130, 166, 1576, 1777, 2374, 2506, 2848, 2916
BspDI	ATCGAT	1 cut(s)	528
BspEI	TCCGGA	1 cut(s)	205
BspHI	TCATGA	1 cut(s)	1345
BspLI	GGNNCC	8 cut(s)	186, 1011, 2000, 2731, 2827, 2917, 2935, 3072
BspMAI	CTGCAG	1 cut(s)	2764
BspOI	GCTAGC	1 cut(s)	2966
BspPI	GGATC	7 cut(s)	197, 524, 1264, 2660, 2671, 2782, 2825
BspT107I	GGYRCC	2 cut(s)	2729, 3070
BsrDI	GCAATG	3 cut(s)	363, 558, 1191
BsrGI	TGTACA	2 cut(s)	1574, 2370
BsrI	ACTGG	8 cut(s)	571, 1309, 1912, 2348, 2733, 2795, 2834, 3065
BssECI	CCNNGG	5 cut(s)	656, 1054, 1371, 2002, 2907
BssNAI	GTATAC	1 cut(s)	45
BssSI	CACGAG	1 cut(s)	810
BssT1I	CCWWGG	3 cut(s)	656, 1054, 1371
Bst1107I	GTATAC	1 cut(s)	45
Bst2BI	CACGAG	1 cut(s)	810
Bst2UI	CCWGG	6 cut(s)	2004, 2022, 2153, 2548, 2567, 2908
Bst6I	CTCTTC	3 cut(s)	1528, 1626, 2484
BstAUI	TGTACA	2 cut(s)	1574, 2370
BstBAI	YACGTR	1 cut(s)	2129
BstC8I	GCNNGC	5 cut(s)	112, 618, 990, 2645, 2964
BstEII	GGTNACC	1 cut(s)	903
BstF5I	GGATG	7 cut(s)	217, 707, 775, 1358, 2052, 2887, 2983
BstHHI	GCGC	1 cut(s)	2643
BstMAI	GTCTC	4 cut(s)	1151, 1283, 1509, 2945
BstMWI	GCNNNNNNNGC	3 cut(s)	362, 1214, 2765
BstNI	CCWGG	6 cut(s)	2004, 2022, 2153, 2548, 2567, 2908
BstNSI	RCATGY	5 cut(s)	620, 992, 1674, 2628, 2647
BstPI	GGTNACC	1 cut(s)	903
BstSCI	CCNGG	7 cut(s)	899, 2002, 2020, 2151, 2546, 2565, 2906
BstSFI	CTRYAG	1 cut(s)	2760
BstSLI	GKGCMC	3 cut(s)	1919, 2099, 3073
BstV1I	GCAGC	5 cut(s)	472, 607, 1987, 2186, 2746
BstV2I	GAAGAC	2 cut(s)	598, 684
BstX2I	RGATCY	6 cut(s)	1559, 2426, 2665, 2774, 2998, 3106
BstYI	RGATCY	6 cut(s)	1559, 2426, 2665, 2774, 2998, 3106
BstZ17I	GTATAC	1 cut(s)	45
Bsu15I	ATCGAT	1 cut(s)	528
Bsu36I	CCTNAGG	1 cut(s)	1584
BsuI	GTATCC	1 cut(s)	2746
BsuRI	GGCC	4 cut(s)	1386, 2205, 2247, 2906
BsuTUI	ATCGAT	1 cut(s)	528
BtgZI	GCGATG	1 cut(s)	857
BtsCI	GGATG	7 cut(s)	217, 707, 775, 1358, 2052, 2887, 2983
BtsI	GCAGTG	3 cut(s)	40, 372, 1127
Cac8I	GCNNGC	5 cut(s)	112, 618, 990, 2645, 2964
CaiI	CAGNNNCTG	1 cut(s)	2768
CciI	TCATGA	1 cut(s)	1345
CfoI	GCGC	1 cut(s)	2643
Cfr13I	GGNCC	5 cut(s)	1009, 2246, 2563, 2804, 2916
ClaI	ATCGAT	1 cut(s)	528
CsiI	ACCWGGT	1 cut(s)	2565
DraI	TTTAAA	3 cut(s)	337, 385, 2470
DraIII	CACNNNGTG	3 cut(s)	128, 1088, 3119
EaeI	YGGCCR	2 cut(s)	2203, 2904
Eam1104I	CTCTTC	3 cut(s)	1528, 1626, 2484
EarI	CTCTTC	3 cut(s)	1528, 1626, 2484
Ecl136II	GAGCTC	1 cut(s)	2158
Eco130I	CCWWGG	3 cut(s)	656, 1054, 1371
Eco24I	GRGCYC	1 cut(s)	2160
Eco47I	GGWCC	4 cut(s)	1009, 2563, 2804, 2916
Eco53kI	GAGCTC	1 cut(s)	2158
Eco57I	CTGAAG	2 cut(s)	764, 2330
Eco72I	CACGTG	1 cut(s)	2129
Eco81I	CCTNAGG	1 cut(s)	1584
Eco88I	CYCGRG	1 cut(s)	1154
Eco91I	GGTNACC	1 cut(s)	903
EcoICRI	GAGCTC	1 cut(s)	2158
EcoO109I	RGGNCCY	3 cut(s)	1009, 2246, 2804
EcoO65I	GGTNACC	1 cut(s)	903
EcoRI	GAATTC	1 cut(s)	2474
EcoRII	CCWGG	6 cut(s)	2002, 2020, 2151, 2546, 2565, 2906
EcoT14I	CCWWGG	3 cut(s)	656, 1054, 1371
EcoT22I	ATGCAT	2 cut(s)	560, 1801
EcoT38I	GRGCYC	1 cut(s)	2160
ErhI	CCWWGG	3 cut(s)	656, 1054, 1371
FalI	AAGNNNNNCTT	8 cut(s)	1230, 1262, 1671, 1703, 2025, 2057, 2331, 2363
FaqI	GGGAC	1 cut(s)	995
FauNDI	CATATG	1 cut(s)	633
FbaI	TGATCA	2 cut(s)	393, 2956
FblI	GTMKAC	2 cut(s)	44, 638
Fnu4HI	GCNGC	6 cut(s)	486, 621, 1976, 2200, 2203, 2760
FokI	GGATG	7 cut(s)	224, 694, 762, 1365, 2059, 2894, 2990
FriOI	GRGCYC	1 cut(s)	2160
Fsp4HI	GCNGC	6 cut(s)	486, 621, 1976, 2200, 2203, 2760
GlaI	GCGC	1 cut(s)	2642
GluI	GCNGC	6 cut(s)	486, 621, 1976, 2200, 2203, 2760
GsuI	CTGGAG	5 cut(s)	327, 1014, 2025, 2174, 2920
HaeIII	GGCC	4 cut(s)	1386, 2205, 2247, 2906
HapII	CCGG	2 cut(s)	206, 900
HhaI	GCGC	1 cut(s)	2643
Hin6I	GCGC	1 cut(s)	2641
HinP1I	GCGC	1 cut(s)	2641
HincII	GTYRAC	3 cut(s)	587, 639, 1174
HindII	GTYRAC	3 cut(s)	587, 639, 1174
HindIII	AAGCTT	2 cut(s)	1680, 2312
HpaII	CCGG	2 cut(s)	206, 900
HphI	GGTGA	6 cut(s)	275, 455, 897, 1705, 2652, 3014
HpyAV	CCTTC	9 cut(s)	308, 721, 1096, 1343, 1376, 2027, 2692, 2795, 3041
HpyCH4IV	ACGT	3 cut(s)	332, 2128, 2400
HpyF10VI	GCNNNNNNNGC	3 cut(s)	362, 1214, 2765
HpySE526I	ACGT	3 cut(s)	332, 2128, 2400
HspAI	GCGC	1 cut(s)	2641
Kpn2I	TCCGGA	1 cut(s)	205
KpnI	GGTACC	1 cut(s)	2733
Ksp22I	TGATCA	2 cut(s)	393, 2956
Lsp1109I	GCAGC	5 cut(s)	472, 607, 1987, 2186, 2746
LweI	GCATC	4 cut(s)	434, 496, 1496, 2484
MabI	ACCWGGT	1 cut(s)	2565
MaeII	ACGT	3 cut(s)	332, 2128, 2400
MaeIII	GTNAC	6 cut(s)	903, 953, 1967, 2278, 2542, 2658
MflI	RGATCY	6 cut(s)	1559, 2426, 2665, 2774, 2998, 3106
MhlI	GDGCHC	6 cut(s)	1084, 1919, 2099, 2160, 2532, 3073
MlsI	TGGCCA	1 cut(s)	2906
MluNI	TGGCCA	1 cut(s)	2906
MlyI	GAGTC	3 cut(s)	307, 950, 2062
MmeI	TCCRAC	1 cut(s)	1737
Mox20I	TGGCCA	1 cut(s)	2906
Mph1103I	ATGCAT	2 cut(s)	560, 1801
MroI	TCCGGA	1 cut(s)	205
MscI	TGGCCA	1 cut(s)	2906
MslI	CAYNNNNRTG	5 cut(s)	129, 758, 780, 1602, 1669
Msp20I	TGGCCA	1 cut(s)	2906
MspA1I	CMGCKG	1 cut(s)	2759
MspI	CCGG	2 cut(s)	206, 900
MspR9I	CCNGG	7 cut(s)	901, 2004, 2022, 2153, 2548, 2567, 2908
Mva1269I	GAATGC	2 cut(s)	1801, 2542
MvaI	CCWGG	6 cut(s)	2004, 2022, 2153, 2548, 2567, 2908
MwoI	GCNNNNNNNGC	3 cut(s)	362, 1214, 2765
NciI	CCSGG	1 cut(s)	901
NdeI	CATATG	1 cut(s)	633
NheI	GCTAGC	1 cut(s)	2962
NlaIV	GGNNCC	8 cut(s)	186, 1011, 2000, 2731, 2827, 2917, 2935, 3072
NmuCI	GTSAC	3 cut(s)	903, 953, 2658
NsiI	ATGCAT	2 cut(s)	560, 1801
NspI	RCATGY	5 cut(s)	620, 992, 1674, 2628, 2647
PaeI	GCATGC	3 cut(s)	620, 992, 2647
PaeR7I	CTCGAG	1 cut(s)	1154
PagI	TCATGA	1 cut(s)	1345
PctI	GAATGC	2 cut(s)	1801, 2542
PfeI	GAWTC	9 cut(s)	652, 1524, 1760, 2085, 2690, 2717, 2816, 2849, 2993
PfoI	TCCNGGA	1 cut(s)	2151
PkrI	GCNGC	6 cut(s)	487, 622, 1977, 2201, 2204, 2761
PleI	GAGTC	3 cut(s)	306, 950, 2061
PmaCI	CACGTG	1 cut(s)	2129
PmlI	CACGTG	1 cut(s)	2129
PpsI	GAGTC	3 cut(s)	306, 950, 2061
Ppu21I	YACGTR	1 cut(s)	2129
PpuMI	RGGWCCY	2 cut(s)	1009, 2804
PshBI	ATTAAT	2 cut(s)	372, 1025
Psp124BI	GAGCTC	1 cut(s)	2160
Psp5II	RGGWCCY	2 cut(s)	1009, 2804
Psp6I	CCWGG	6 cut(s)	2002, 2020, 2151, 2546, 2565, 2906
PspCI	CACGTG	1 cut(s)	2129
PspEI	GGTNACC	1 cut(s)	903
PspGI	CCWGG	6 cut(s)	2002, 2020, 2151, 2546, 2565, 2906
PspN4I	GGNNCC	8 cut(s)	186, 1011, 2000, 2731, 2827, 2917, 2935, 3072
PspPI	GGNCC	5 cut(s)	1009, 2246, 2563, 2804, 2916
PspPPI	RGGWCCY	2 cut(s)	1009, 2804
PsrI	GAACNNNNNNTAC	2 cut(s)	274, 306
PstI	CTGCAG	1 cut(s)	2764
PstNI	CAGNNNCTG	1 cut(s)	2768
PsuI	RGATCY	6 cut(s)	1559, 2426, 2665, 2774, 2998, 3106
PvuII	CAGCTG	1 cut(s)	2759
RseI	CAYNNNNRTG	5 cut(s)	129, 758, 780, 1602, 1669
SacI	GAGCTC	1 cut(s)	2160
SalI	GTCGAC	1 cut(s)	637
SatI	GCNGC	6 cut(s)	486, 621, 1976, 2200, 2203, 2760
Sau96I	GGNCC	5 cut(s)	1009, 2246, 2563, 2804, 2916
ScaI	AGTACT	2 cut(s)	681, 2601
SchI	GAGTC	3 cut(s)	307, 950, 2062
ScrFI	CCNGG	7 cut(s)	901, 2004, 2022, 2153, 2548, 2567, 2908
SduI	GDGCHC	6 cut(s)	1084, 1919, 2099, 2160, 2532, 3073
SexAI	ACCWGGT	1 cut(s)	2565
SfaNI	GCATC	4 cut(s)	434, 496, 1496, 2484
SfcI	CTRYAG	1 cut(s)	2760
Sfr274I	CTCGAG	1 cut(s)	1154
SinI	GGWCC	4 cut(s)	1009, 2563, 2804, 2916
SlaI	CTCGAG	1 cut(s)	1154
SmiMI	CAYNNNNRTG	5 cut(s)	129, 758, 780, 1602, 1669
SmlI	CTYRAG	3 cut(s)	1154, 1688, 2531
SmoI	CTYRAG	3 cut(s)	1154, 1688, 2531
SpeI	ACTAGT	3 cut(s)	116, 589, 1292
SphI	GCATGC	3 cut(s)	620, 992, 2647
SsiI	CCGC	2 cut(s)	1418, 2202
SstI	GAGCTC	1 cut(s)	2160
StyD4I	CCNGG	7 cut(s)	899, 2002, 2020, 2151, 2546, 2565, 2906
StyI	CCWWGG	3 cut(s)	656, 1054, 1371
TaiI	ACGT	3 cut(s)	335, 2131, 2403
TatI	WGTACW	5 cut(s)	645, 679, 1574, 2370, 2599
TauI	GCSGC	1 cut(s)	2205
TfiI	GAWTC	9 cut(s)	652, 1524, 1760, 2085, 2690, 2717, 2816, 2849, 2993
TseFI	GTSAC	3 cut(s)	903, 953, 2658
TseI	GCWGC	5 cut(s)	485, 620, 1975, 2199, 2759
Tsp45I	GTSAC	3 cut(s)	903, 953, 2658
TspGWI	ACGGA	2 cut(s)	1099, 2567
VneI	GTGCAC	2 cut(s)	1915, 2095
VpaK11BI	GGWCC	4 cut(s)	1009, 2563, 2804, 2916
VspI	ATTAAT	2 cut(s)	372, 1025
XapI	RAATTY	6 cut(s)	148, 1474, 1855, 2146, 2474, 3084
XbaI	TCTAGA	1 cut(s)	2684
XceI	RCATGY	5 cut(s)	620, 992, 1674, 2628, 2647
XhoI	CTCGAG	1 cut(s)	1154
XmaJI	CCTAGG	1 cut(s)	1054
XmiI	GTMKAC	2 cut(s)	44, 638
ZrmI	AGTACT	2 cut(s)	681, 2601
Zsp2I	ATGCAT	2 cut(s)	560, 1801

Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.