pycom10g05220

Essential component of the vacuolar proton pump (V- ATPase), a multimeric enzyme that catalyzes the translocation of protons across the membranes. Required for assembly and activity of the V-ATPase

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
pyrus_communis
Chr10
OG0003618
Physical Location & Seq
Forward (+)
5584202 .. 5593953
9752 bp
Loading structure...
UTR
Exon/CDS
Intron
N/A

Sequence Viewer

Length: 2445 bp
ATGGGAGAGTGCTGTCCTCCGATGGATCTGTTCAGGTCGGAGCCGATGCAGCTCGTCCAGATCATCATCCCCATAGAGTCCGCTCACCCTACCGTCTCTTATCTCGGCGACCTCGGTCTCATCCAGTTCAAAGACCTCAATTCAGAGAAGAGCCCGTTTCAGAGGACATATGCTGCTCAGATTAAAAGAAGTGCAGAGATGGCACGCAAATTACGTTTCTTTAAGGAGAAAATGTCAAAGGCAGATTTACCTCCTTCAAAGTCTGCAAGACAAGTTGATGTCAATGTGGATGACCTAGAGGTAAAACTTGGTGAACTTGAGGCAGAGCTGATTGAAATAAATTTGAATGATGAAAAGTTGCAACGTTCATATAATGAACTCTTGGAATATAAGCTTGTTCTGGAAAAGGCTGGTGAGTTTTTTCACGCAGCTCGAAGCAGTGCCGTGTTGCATCAGAGAGAAAATGAATCCCGTGATATCGGTGATGAATCCCTGGACATGCCTTTGTTATTGGAGCAAGAAACATCAACCGATCCATCAAAGCAAGTGAAGTTGGGATTTCTCACTGGTCTTGTTCCCAGGGAAAAGTCTTTGGCATTTGAGAGGATTCTATTTCGAGCTACTCGGGGTAATGTTTTTCTGAGGCAGACTGTTGTTGAGAATCCCGTGATTGATCCTGTTTCAGGAGAGAAGGTTGAGAAAAATGTTTTTGTGGTATTCTACTCTGGGGAAAGAGCAAAGAGCAAAATTCTCAAAATATGTGATGCCTTTGGTGCTAATCGATACTCTTTTACTGAGGACCTAGGTAGACAATATCAGATGATTACTGAGGTTTCGGGAAGACTTTCAGAGCTAAAGACTACCATAGATGTTGGAGTGTTACTTCGGGGAAGTTTGTTACAGACTATTGGCGAGCATTTTGAGCAGTGGAACCTTTTGGTGAGGAAGGAGAAATCCATATACCACCATCTTAACATGCTTAGCCTTGATGTTACAAAAAAATGTCTTGTGGCTGAAGGGTGGAGTCCTGTTTTTGCATCAAAACAGATCCAAGATGCATTGCAGCGGGCAGCATTTGATTCCAACTCACAAGTTGGAGCTATTTTCCAGGTTTTGCATGCTAAGGAGGCACCTCCTACCTATTTCCGTACAAACAAATTCACTTCTTCTTTTCAAGAAATTGTTGATGCATACGGGGTGGCTAAGTATCAAGAAGCAAATCCTGCTGTATATACTATCGTGACATTCCCGTTTCTTTTTGCTGTCATGTTTGGTGATTGGGGACATGGAATATGTTTGTTACTTGCAGCCTTATATTTAATAGGCAGGGAAAAGAAGCTTTCTAGTCAGAAGCTGGGAGACATCATGGAGATGGCCTTTGGTGGTCGTTATGTGATTTTGTTGATGGCAATTTTCTCAATCTACACAGGTTTTATCTATAATGAGTTCTTCTCTGTCCCGTTCGAACTGTTTGGTCCCTCAGCATATGCATGCCGTGATCTTTCTTGCAGAGATGCTACTACAGCTGGCTTGATTAAGGTGCGGCCCGCATACCCATTTGGCCTGGATCCTGTATGGCATGGATCCCGCAGTGAGCTTCCATTTCTAAACTCATTGAAGATGAAAATGTCAATTATTATTGGCGTGGTCCAGATGAACCTGGGAATTATGATCAGTTATATCAATGCCAGGTTCTTTCGAAGTAATTTGAATGTTTGGTTCCAGTTTATACCCCAGATGATATTTTTAAACGGTCTATTTGGCTACTTGTCTGTTCTCATTGTTATGAAGTGGTGGACTGGTTCAAAAGCTGATCTCTACCATGTAATGATATACATGTTCCTAAGTCCTACAGACGAGCTAGGTGAAAATCAGCTTTTCAATGGTCAGAAAACAGTTCAGCTTGTACTATTATTGTTGGCTTTTGTTGCTGTACCATGGATGCTGTTTCCAAAGCCTTTTCTTTTGAAGAAGCAACACCAAGATCGGCATCAAGGTCAATCCTATGCTCTACTTGAGAACACTGAAGAGAGCTTGCAAGTGAATTCAAACCATGATGCCCATGGTCATGAGGAGTTTGAGTTTAGTGAAGTTTTCGTACATCAAATGATACACACAATAGAATTTGTACTTGGAGCAGTCTCAAATACAGCTTCTTACCTTCGTCTATGGGCTTTAAGTCTTGCTCATTCAGAGTTGTCCAGTGTGTTCTACGACAAGGTTCTCCTAACAGCCTGGGGGTTCAACAATTGGATTATACTCATAGTGGGTCTCATCGTCTTTGTGTGCGCCACTGTCGGTGTTTTACTACTGATGGAAACCCTCAGTGCTTTCCTTCACGCTCTTCGACTTCACTGGGTGGAATTCCAGAACAAGTTTTATGAAGGGGATGGTTACAAATTCTATCCGTACTCGTTCGCACTGCTTGACGACGACGATGAGTGA
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
GO:0000323 GO:0000325 GO:0000331 GO:0003674 GO:0003824 GO:0005215 GO:0005488 GO:0005515 GO:0005575 GO:0005622 GO:0005623 GO:0005737 GO:0005764 GO:0005765 GO:0005768 GO:0005773 GO:0005774 GO:0005794 GO:0005886 GO:0006139 GO:0006163 GO:0006164 GO:0006725 GO:0006753 GO:0006754 GO:0006793 GO:0006796 GO:0006807 GO:0006810 GO:0006811 GO:0006812 GO:0006873 GO:0006875 GO:0006882 GO:0006885 GO:0006897 GO:0006909 GO:0006911 GO:0007035 GO:0008150 GO:0008152 GO:0008324 GO:0009058 GO:0009117 GO:0009123 GO:0009124 GO:0009126 GO:0009127 GO:0009141 GO:0009142 GO:0009144 GO:0009145 GO:0009150 GO:0009152 GO:0009156 GO:0009161 GO:0009165 GO:0009167 GO:0009168 GO:0009199 GO:0009201 GO:0009205 GO:0009206 GO:0009259 GO:0009260 GO:0009507 GO:0009526 GO:0009536 GO:0009678 GO:0009705 GO:0009941 GO:0009987 GO:0010008 GO:0010324 GO:0012505 GO:0012506 GO:0015075 GO:0015077 GO:0015078 GO:0015318 GO:0015399 GO:0015405 GO:0015672 GO:0015985 GO:0015986 GO:0016020 GO:0016043 GO:0016192 GO:0016462 GO:0016469 GO:0016471 GO:0016787 GO:0016817 GO:0016818 GO:0016887 GO:0017111 GO:0017144 GO:0018130 GO:0019438 GO:0019637 GO:0019693 GO:0019725 GO:0019829 GO:0019899 GO:0022607 GO:0022804 GO:0022853 GO:0022857 GO:0022890 GO:0030003 GO:0030004 GO:0030139 GO:0030641 GO:0030659 GO:0030666 GO:0030670 GO:0031090 GO:0031164 GO:0031410 GO:0031967 GO:0031975 GO:0031982 GO:0032009 GO:0032119 GO:0032991 GO:0033176 GO:0034220 GO:0034622 GO:0034641 GO:0034654 GO:0036442 GO:0042592 GO:0042623 GO:0042625 GO:0042626 GO:0043181 GO:0043226 GO:0043227 GO:0043229 GO:0043231 GO:0043492 GO:0043933 GO:0044085 GO:0044237 GO:0044238 GO:0044249 GO:0044271 GO:0044281 GO:0044422 GO:0044424 GO:0044425 GO:0044433 GO:0044434 GO:0044435 GO:0044437 GO:0044440 GO:0044444 GO:0044446 GO:0044464 GO:0044769 GO:0045335 GO:0045735 GO:0045851 GO:0046034 GO:0046390 GO:0046483 GO:0046916 GO:0046961 GO:0048878 GO:0050801 GO:0051117 GO:0051179 GO:0051234 GO:0051235 GO:0051238 GO:0051452 GO:0051453 GO:0051641 GO:0051651 GO:0055065 GO:0055067 GO:0055069 GO:0055076 GO:0055080 GO:0055082 GO:0055085 GO:0055086 GO:0061024 GO:0065003 GO:0065007 GO:0065008 GO:0070070 GO:0070071 GO:0070072 GO:0071704 GO:0071840 GO:0071944 GO:0072503 GO:0072507 GO:0072521 GO:0072522 GO:0090407 GO:0090662 GO:0097708 GO:0098588 GO:0098655 GO:0098657 GO:0098660 GO:0098662 GO:0098771 GO:0098796 GO:0098805 GO:0098852 GO:0099024 GO:0099131 GO:0099132 GO:1901135 GO:1901137 GO:1901293 GO:1901360 GO:1901362 GO:1901564 GO:1901566 GO:1901576 GO:1902600
KEGG Pathways
Metabolic & Signaling
ko00190 ko01100 ko04142 ko04145 ko04721 ko04966 ko05110 ko05120 ko05152 ko05165 ko05323 map00190 map01100 map04142 map04145 map04721 map04966 map05110 map05120 map05152 map05165 map05323
Pfam Domains
Protein Families
V_ATPase_I

Protein Analysis

815

Amino Acids

92.78

Weight (kDa)

5.86

Isoelectric Point (pI)

38.88

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
V_ATPase_I PF01496 34 - 806 5.9e-301 V-type ATPase 116kDa subunit family
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AccB1I GGYRCC 1 cut(s) 1129
AccBSI CCGCTC 1 cut(s) 83
AccI GTMKAC 1 cut(s) 808
AciI CCGC 5 cut(s) 81, 1066, 1543, 1548, 1588
AclI AACGTT 1 cut(s) 364
AclWI GGATC 8 cut(s) 33, 527, 668, 1042, 1562, 1575, 1578, 1591
AcsI RAATTY 7 cut(s) 340, 747, 1157, 2044, 2123, 2363, 2399
AcuI CTGAAG 2 cut(s) 1035, 2046
AdeI CACNNNGTG 1 cut(s) 2359
AfaI GTAC 6 cut(s) 1150, 1908, 1935, 2100, 2130, 2411
AfiI CCNNNNNNNGG 1 cut(s) 683
AflIII ACRYGT 1 cut(s) 1836
AjnI CCWGG 7 cut(s) 492, 578, 1107, 1563, 1659, 1688, 2234
AjuI GAANNNNNNNTTGG 2 cut(s) 2115, 2147
Alw26I GTCTC 5 cut(s) 100, 122, 1353, 2146, 2276
AlwI GGATC 8 cut(s) 33, 527, 668, 1042, 1562, 1575, 1578, 1591
AlwNI CAGNNNCTG 1 cut(s) 1354
Ama87I CYCGRG 1 cut(s) 624
AoxI GGCC 3 cut(s) 1374, 1544, 1561
ApeKI GCWGC 6 cut(s) 49, 173, 428, 1063, 1070, 1307
ApoI RAATTY 7 cut(s) 340, 747, 1157, 2044, 2123, 2363, 2399
Asp700I GAANNNNTTC 1 cut(s) 844
AspA2I CCTAGG 1 cut(s) 802
AspLEI GCGC 1 cut(s) 2291
AspS9I GGNCC 4 cut(s) 799, 1475, 1545, 1648
AsuHPI GGTGA 7 cut(s) 77, 323, 425, 494, 952, 1286, 1877
AsuII TTCGAA 2 cut(s) 1464, 1699
AvaI CYCGRG 1 cut(s) 624
AvaII GGWCC 3 cut(s) 799, 1475, 1648
AvrII CCTAGG 1 cut(s) 802
BamHI GGATCC 2 cut(s) 1567, 1583
BanI GGYRCC 1 cut(s) 1129
BanII GRGCYC 1 cut(s) 155
BarI GAAGNNNNNNTAC 2 cut(s) 2082, 2114
BbsI GAAGAC 1 cut(s) 847
BbvCI CCTCAGC 1 cut(s) 1480
BbvI GCAGC 6 cut(s) 61, 160, 440, 1075, 1082, 1319
BccI CCATC 8 cut(s) 16, 193, 544, 975, 1366, 1399, 2308, 2384
BceAI ACGGC 2 cut(s) 428, 1479
BciT130I CCWGG 7 cut(s) 494, 580, 1109, 1565, 1661, 1690, 2236
BclI TGATCA 1 cut(s) 1671
BcoDI GTCTC 5 cut(s) 100, 122, 1353, 2146, 2276
BfaI CTAG 4 cut(s) 296, 803, 1344, 1862
BfmI CTRYAG 2 cut(s) 1521, 1851
BisI GCNGC 7 cut(s) 50, 174, 429, 1064, 1071, 1308, 1544
BlnI CCTAGG 1 cut(s) 802
BlpI GCTNAGC 1 cut(s) 980
BlsI GCNGC 7 cut(s) 51, 175, 430, 1065, 1072, 1309, 1545
Bme1390I CCNGG 7 cut(s) 494, 580, 1109, 1565, 1661, 1690, 2236
Bme18I GGWCC 3 cut(s) 799, 1475, 1648
BmeT110I CYCGRG 1 cut(s) 624
BmgT120I GGNCC 4 cut(s) 799, 1475, 1545, 1648
BmiI GGNNCC 7 cut(s) 42, 932, 1131, 1477, 1569, 1585, 1721
BmrFI CCNGG 7 cut(s) 494, 580, 1109, 1565, 1661, 1690, 2236
BmrI ACTGGG 1 cut(s) 2365
BmuI ACTGGG 1 cut(s) 2365
BoxI GACNNNNGTC 1 cut(s) 114
BpiI GAAGAC 1 cut(s) 847
BplI GAGNNNNNCTC 2 cut(s) 1436, 1468
Bpu10I CCTNAGC 2 cut(s) 1122, 1480
Bpu1102I GCTNAGC 1 cut(s) 980
Bpu14I TTCGAA 2 cut(s) 1464, 1699
BpuEI CTTGAG 2 cut(s) 338, 2036
Bsa29I ATCGAT 1 cut(s) 781
BsaBI GATNNNNATC 2 cut(s) 65, 1989
BsaI GGTCTC 2 cut(s) 122, 2276
BsaJI CCNNGG 9 cut(s) 112, 492, 578, 579, 802, 1660, 1937, 2062, 2235
BsaXI ACNNNNNCTCC 6 cut(s) 218, 248, 678, 708, 2066, 2096
Bsc4I CCNNNNNNNGG 1 cut(s) 683
Bse1I ACTGG 6 cut(s) 124, 571, 1723, 1804, 2202, 2360
Bse3DI GCAATG 1 cut(s) 1058
Bse8I GATNNNNATC 2 cut(s) 65, 1989
BseBI CCWGG 7 cut(s) 494, 580, 1109, 1565, 1661, 1690, 2236
BseCI ATCGAT 1 cut(s) 781
BseDI CCNNGG 9 cut(s) 112, 492, 578, 579, 802, 1660, 1937, 2062, 2235
BseGI GGATG 5 cut(s) 66, 120, 295, 1947, 2395
BseJI GATNNNNATC 2 cut(s) 65, 1989
BseLI CCNNNNNNNGG 1 cut(s) 683
BseMI GCAATG 1 cut(s) 1058
BseMII CTCAG 6 cut(s) 191, 632, 786, 819, 1494, 2338
BseNI ACTGG 6 cut(s) 124, 571, 1723, 1804, 2202, 2360
BseRI GAGGAG 1 cut(s) 2087
BseXI GCAGC 6 cut(s) 61, 160, 440, 1075, 1082, 1319
BseYI CCCAGC 1 cut(s) 1354
BsgI GTGCAG 1 cut(s) 213
BshFI GGCC 3 cut(s) 1376, 1546, 1563
BshNI GGYRCC 1 cut(s) 1129
BshVI ATCGAT 1 cut(s) 781
BsiHKCI CYCGRG 1 cut(s) 624
BslFI GGGAC 3 cut(s) 1296, 1442, 1461
BslI CCNNNNNNNGG 1 cut(s) 683
BsmAI GTCTC 5 cut(s) 100, 122, 1353, 2146, 2276
BsmBI CGTCTC 1 cut(s) 100
BsmFI GGGAC 3 cut(s) 1296, 1442, 1461
BsnI GGCC 3 cut(s) 1376, 1546, 1563
Bso31I GGTCTC 2 cut(s) 122, 2276
BsoBI CYCGRG 1 cut(s) 624
Bsp119I TTCGAA 2 cut(s) 1464, 1699
Bsp1286I GDGCHC 1 cut(s) 155
Bsp1720I GCTNAGC 1 cut(s) 980
Bsp19I CCATGG 2 cut(s) 1937, 2062
BspACI CCGC 5 cut(s) 81, 1066, 1543, 1548, 1588
BspANI GGCC 3 cut(s) 1376, 1546, 1563
BspCNI CTCAG 6 cut(s) 190, 633, 787, 820, 1493, 2337
BspDI ATCGAT 1 cut(s) 781
BspHI TCATGA 1 cut(s) 2068
BspLI GGNNCC 7 cut(s) 42, 932, 1131, 1477, 1569, 1585, 1721
BspPI GGATC 8 cut(s) 33, 527, 668, 1042, 1562, 1575, 1578, 1591
BspQI GCTCTTC 2 cut(s) 143, 2349
BspT104I TTCGAA 2 cut(s) 1464, 1699
BspT107I GGYRCC 1 cut(s) 1129
BspTNI GGTCTC 2 cut(s) 122, 2276
BsrBI CCGCTC 1 cut(s) 83
BsrDI GCAATG 1 cut(s) 1058
BsrI ACTGG 6 cut(s) 124, 571, 1723, 1804, 2202, 2360
BssECI CCNNGG 9 cut(s) 112, 492, 578, 579, 802, 1660, 1937, 2062, 2235
BssT1I CCWWGG 3 cut(s) 802, 1937, 2062
Bst2UI CCWGG 7 cut(s) 494, 580, 1109, 1565, 1661, 1690, 2236
Bst4CI ACNGT 6 cut(s) 94, 652, 1470, 1754, 1897, 2296
Bst6I CTCTTC 3 cut(s) 143, 2022, 2349
BstAPI GCANNNNNTGC 1 cut(s) 1223
BstBI TTCGAA 2 cut(s) 1464, 1699
BstC8I GCNNGC 8 cut(s) 205, 914, 1068, 1119, 1492, 1528, 1548, 2036
BstDSI CCRYGG 2 cut(s) 1937, 2062
BstENI CCTNNNNNAGG 1 cut(s) 681
BstF5I GGATG 5 cut(s) 66, 120, 295, 1947, 2395
BstHHI GCGC 1 cut(s) 2291
BstMAI GTCTC 5 cut(s) 100, 122, 1353, 2146, 2276
BstMWI GCNNNNNNNGC 8 cut(s) 49, 200, 773, 922, 1127, 1223, 1523, 1928
BstNI CCWGG 7 cut(s) 494, 580, 1109, 1565, 1661, 1690, 2236
BstNSI RCATGY 5 cut(s) 502, 979, 1121, 1494, 1840
BstPAI GACNNNNGTC 1 cut(s) 114
BstSCI CCNGG 7 cut(s) 492, 578, 1107, 1563, 1659, 1688, 2234
BstSFI CTRYAG 2 cut(s) 1521, 1851
BstV1I GCAGC 6 cut(s) 61, 160, 440, 1075, 1082, 1319
BstV2I GAAGAC 1 cut(s) 847
BstX2I RGATCY 4 cut(s) 25, 1047, 1567, 1583
BstYI RGATCY 4 cut(s) 25, 1047, 1567, 1583
Bsu15I ATCGAT 1 cut(s) 781
BsuRI GGCC 3 cut(s) 1376, 1546, 1563
BsuTUI ATCGAT 1 cut(s) 781
BtgI CCRYGG 2 cut(s) 1937, 2062
BtsCI GGATG 5 cut(s) 66, 120, 295, 1947, 2395
BtsI GCAGTG 4 cut(s) 445, 932, 1597, 2420
Cac8I GCNNGC 8 cut(s) 205, 914, 1068, 1119, 1492, 1528, 1548, 2036
CaiI CAGNNNCTG 1 cut(s) 1354
CciI TCATGA 1 cut(s) 2068
CfoI GCGC 1 cut(s) 2291
Cfr13I GGNCC 4 cut(s) 799, 1475, 1545, 1648
ClaI ATCGAT 1 cut(s) 781
Csp6I GTAC 6 cut(s) 1149, 1907, 1934, 2099, 2129, 2410
CviQI GTAC 6 cut(s) 1149, 1907, 1934, 2099, 2129, 2410
DraI TTTAAA 1 cut(s) 1749
DraIII CACNNNGTG 1 cut(s) 2359
Eam1104I CTCTTC 3 cut(s) 143, 2022, 2349
EarI CTCTTC 3 cut(s) 143, 2022, 2349
Eco130I CCWWGG 3 cut(s) 802, 1937, 2062
Eco24I GRGCYC 1 cut(s) 155
Eco31I GGTCTC 2 cut(s) 122, 2276
Eco32I GATATC 1 cut(s) 478
Eco47I GGWCC 3 cut(s) 799, 1475, 1648
Eco57I CTGAAG 2 cut(s) 1035, 2046
Eco88I CYCGRG 1 cut(s) 624
EcoNI CCTNNNNNAGG 1 cut(s) 681
EcoO109I RGGNCCY 1 cut(s) 799
EcoRI GAATTC 2 cut(s) 2044, 2363
EcoRII CCWGG 7 cut(s) 492, 578, 1107, 1563, 1659, 1688, 2234
EcoRV GATATC 1 cut(s) 478
EcoT14I CCWWGG 3 cut(s) 802, 1937, 2062
EcoT22I ATGCAT 3 cut(s) 1060, 1192, 1492
EcoT38I GRGCYC 1 cut(s) 155
ErhI CCWWGG 3 cut(s) 802, 1937, 2062
Esp3I CGTCTC 1 cut(s) 100
FaqI GGGAC 3 cut(s) 1296, 1442, 1461
FauI CCCGC 3 cut(s) 1059, 1555, 1595
FauNDI CATATG 2 cut(s) 169, 1486
FbaI TGATCA 1 cut(s) 1671
FblI GTMKAC 1 cut(s) 808
Fnu4HI GCNGC 7 cut(s) 50, 174, 429, 1064, 1071, 1308, 1544
FokI GGATG 5 cut(s) 53, 107, 302, 1954, 2402
FriOI GRGCYC 1 cut(s) 155
Fsp4HI GCNGC 7 cut(s) 50, 174, 429, 1064, 1071, 1308, 1544
FspBI CTAG 4 cut(s) 296, 803, 1344, 1862
GlaI GCGC 1 cut(s) 2290
GluI GCNGC 7 cut(s) 50, 174, 429, 1064, 1071, 1308, 1544
GsaI CCCAGC 1 cut(s) 1358
HaeIII GGCC 3 cut(s) 1376, 1546, 1563
HhaI GCGC 1 cut(s) 2291
Hin6I GCGC 1 cut(s) 2289
HinP1I GCGC 1 cut(s) 2289
HindIII AAGCTT 2 cut(s) 392, 1337
HinfI GANTC 7 cut(s) 77, 467, 488, 607, 661, 1024, 1079
HphI GGTGA 7 cut(s) 77, 323, 425, 494, 952, 1286, 1877
Hpy166II GTNNAC 3 cut(s) 314, 809, 1797
Hpy8I GTNNAC 3 cut(s) 314, 809, 1797
Hpy99I CGWCG 2 cut(s) 2435, 2438
HpyAV CCTTC 7 cut(s) 264, 685, 940, 1010, 2171, 2345, 2378
HpyCH4III ACNGT 6 cut(s) 94, 652, 1470, 1754, 1897, 2296
HpyCH4IV ACGT 2 cut(s) 214, 364
HpyF10VI GCNNNNNNNGC 8 cut(s) 49, 200, 773, 922, 1127, 1223, 1523, 1928
HpySE526I ACGT 2 cut(s) 214, 364
HspAI GCGC 1 cut(s) 2289
Ksp22I TGATCA 1 cut(s) 1671
LguI GCTCTTC 2 cut(s) 143, 2349
LmnI GCTCC 4 cut(s) 40, 514, 1097, 2135
Lsp1109I GCAGC 6 cut(s) 61, 160, 440, 1075, 1082, 1319
MaeI CTAG 4 cut(s) 296, 803, 1344, 1862
MaeII ACGT 2 cut(s) 214, 364
MaeIII GTNAC 6 cut(s) 879, 897, 991, 1240, 1299, 2393
MbiI CCGCTC 1 cut(s) 83
MboII GAAGA 8 cut(s) 160, 852, 1158, 1441, 1630, 1981, 2039, 2336
MfeI CAATTG 1 cut(s) 2248
MflI RGATCY 4 cut(s) 25, 1047, 1567, 1583
MhlI GDGCHC 1 cut(s) 155
MlyI GAGTC 2 cut(s) 86, 1033
MmeI TCCRAC 4 cut(s) 18, 853, 1075, 1107
Mph1103I ATGCAT 3 cut(s) 1060, 1192, 1492
MroXI GAANNNNTTC 1 cut(s) 844
MseI TTAA 7 cut(s) 183, 222, 972, 1319, 1536, 1748, 2177
MslI CAYNNNNRTG 4 cut(s) 1370, 1489, 1784, 2067
MspA1I CMGCKG 2 cut(s) 1066, 1526
MspR9I CCNGG 7 cut(s) 494, 580, 1109, 1565, 1661, 1690, 2236
MunI CAATTG 1 cut(s) 2248
MvaI CCWGG 7 cut(s) 494, 580, 1109, 1565, 1661, 1690, 2236
MwoI GCNNNNNNNGC 8 cut(s) 49, 200, 773, 922, 1127, 1223, 1523, 1928
NcoI CCATGG 2 cut(s) 1937, 2062
NdeI CATATG 2 cut(s) 169, 1486
NlaIV GGNNCC 7 cut(s) 42, 932, 1131, 1477, 1569, 1585, 1721
NmeAIII GCCGAG 1 cut(s) 84
NmuCI GTSAC 1 cut(s) 1240
NsiI ATGCAT 3 cut(s) 1060, 1192, 1492
NspI RCATGY 5 cut(s) 502, 979, 1121, 1494, 1840
NspV TTCGAA 2 cut(s) 1464, 1699
PaeI GCATGC 2 cut(s) 1121, 1494
PagI TCATGA 1 cut(s) 2068
PasI CCCWGGG 1 cut(s) 579
PciI ACATGT 1 cut(s) 1836
PciSI GCTCTTC 2 cut(s) 143, 2349
PcsI WCGNNNNNNNCGW 1 cut(s) 211
PdmI GAANNNNTTC 1 cut(s) 844
PfeI GAWTC 5 cut(s) 467, 488, 607, 661, 1079
PkrI GCNGC 7 cut(s) 51, 175, 430, 1065, 1072, 1309, 1545
PleI GAGTC 2 cut(s) 85, 1032
PpsI GAGTC 2 cut(s) 85, 1032
PpuMI RGGWCCY 1 cut(s) 799
PscI ACATGT 1 cut(s) 1836
PshAI GACNNNNGTC 1 cut(s) 114
Psp1406I AACGTT 1 cut(s) 364
Psp5II RGGWCCY 1 cut(s) 799
Psp6I CCWGG 7 cut(s) 492, 578, 1107, 1563, 1659, 1688, 2234
PspFI CCCAGC 1 cut(s) 1354
PspGI CCWGG 7 cut(s) 492, 578, 1107, 1563, 1659, 1688, 2234
PspN4I GGNNCC 7 cut(s) 42, 932, 1131, 1477, 1569, 1585, 1721
PspPI GGNCC 4 cut(s) 799, 1475, 1545, 1648
PspPPI RGGWCCY 1 cut(s) 799
PstNI CAGNNNCTG 1 cut(s) 1354
PsuI RGATCY 4 cut(s) 25, 1047, 1567, 1583
PvuII CAGCTG 1 cut(s) 1526
RsaI GTAC 6 cut(s) 1150, 1908, 1935, 2100, 2130, 2411
RsaNI GTAC 6 cut(s) 1149, 1907, 1934, 2099, 2129, 2410
RseI CAYNNNNRTG 4 cut(s) 1370, 1489, 1784, 2067
SapI GCTCTTC 2 cut(s) 143, 2349
SaqAI TTAA 7 cut(s) 183, 222, 972, 1319, 1536, 1748, 2177
SatI GCNGC 7 cut(s) 50, 174, 429, 1064, 1071, 1308, 1544
Sau96I GGNCC 4 cut(s) 799, 1475, 1545, 1648
SchI GAGTC 2 cut(s) 86, 1033
ScrFI CCNGG 7 cut(s) 494, 580, 1109, 1565, 1661, 1690, 2236
SduI GDGCHC 1 cut(s) 155
SfcI CTRYAG 2 cut(s) 1521, 1851
SfuI TTCGAA 2 cut(s) 1464, 1699
SinI GGWCC 3 cut(s) 799, 1475, 1648
SmiMI CAYNNNNRTG 4 cut(s) 1370, 1489, 1784, 2067
SmlI CTYRAG 2 cut(s) 317, 2015
SmoI CTYRAG 2 cut(s) 317, 2015
SphI GCATGC 2 cut(s) 1121, 1494
SsiI CCGC 5 cut(s) 81, 1066, 1543, 1548, 1588
SspMI CTAG 4 cut(s) 296, 803, 1344, 1862
StyD4I CCNGG 7 cut(s) 492, 578, 1107, 1563, 1659, 1688, 2234
StyI CCWWGG 3 cut(s) 802, 1937, 2062
TaaI ACNGT 6 cut(s) 94, 652, 1470, 1754, 1897, 2296
TaiI ACGT 2 cut(s) 217, 367
TaqI TCGA 6 cut(s) 433, 616, 781, 1464, 1699, 2347
TaqII GACCGA 1 cut(s) 104
TatI WGTACW 2 cut(s) 1906, 2128
TauI GCSGC 1 cut(s) 1546
TfiI GAWTC 5 cut(s) 467, 488, 607, 661, 1079
Tru1I TTAA 7 cut(s) 183, 222, 972, 1319, 1536, 1748, 2177
Tru9I TTAA 7 cut(s) 183, 222, 972, 1319, 1536, 1748, 2177
TseFI GTSAC 1 cut(s) 1240
TseI GCWGC 6 cut(s) 49, 173, 428, 1063, 1070, 1307
Tsp45I GTSAC 1 cut(s) 1240
TspDTI ATGAA 9 cut(s) 357, 366, 390, 480, 501, 1637, 1670, 1802, 2397
TspGWI ACGGA 2 cut(s) 1136, 2397
VpaK11BI GGWCC 3 cut(s) 799, 1475, 1648
XagI CCTNNNNNAGG 1 cut(s) 681
XapI RAATTY 7 cut(s) 340, 747, 1157, 2044, 2123, 2363, 2399
XceI RCATGY 5 cut(s) 502, 979, 1121, 1494, 1840
XcmI CCANNNNNNNNNTGG 1 cut(s) 2060
XmaJI CCTAGG 1 cut(s) 802
XmiI GTMKAC 1 cut(s) 808
XmnI GAANNNNTTC 1 cut(s) 844
XspI CTAG 4 cut(s) 296, 803, 1344, 1862
Zsp2I ATGCAT 3 cut(s) 1060, 1192, 1492
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.