Basic Information

Type: gene

Biological Identity

Species

pyrus_communis

Chromosome

Chr10

Orthogroup ID

OG0004305

Physical Location & Seq

Genomic Coordinates Forward (+)

13959449 .. 13962029

Gene Structure 2581 bp

Loading structure...

UTR

Exon/CDS

Intron

Transcript / Protein ID

N/A

Sequence Viewer

CDS Protein Promoter (2k)

Length: 2499 bp

ATGCAATGTCGATTCTCTTGTTTCGAGGCCCCGCGCAGTAAATGGGCTGACTCGTCTACGACAAAATTATCCACTCACACTCCCAAAAGAATAATAATCTCACTTATAACACTCCCCTCCTCTCGTCTGTCGGTCACACACAGTTGTTCCACTTCACTCTCACTCTCTCTCTGTCCCCTTCCAATGGCCTCCAATCCTCTCCTCCCCCTCCTCTTGCTTCTCACATTATTCAAATTCTCAGCCCAAATCGCGCCGCAGGCCGCCGATCAAACCCTCAAGACCTTCATTTTCCGCGTCGACCGCCACTCCAAGCCCTCGATTTTCCCCACCCACTACCACTGGTACGCCTCCGAATTCGCCGACCCGCCCCAGATCCTCCACGTCTACGACACCGTTTTCCACGGCTTCTCCGCCTCTCTCACTCCCGACCAGGTCTCCGCCATCTCCGACCACCCTTCGGTGCTTGCTGTGATCCAGGACCGCCGCCGTAGTCTCCACACCACTCGATCCCCACAATTTTTGGGGCTCAGGAACCAGAGGGGGCTGTGGTCGGAATCGGACTATGGCTCCGACGTCATTGTCGGAGTATTTGACACCGGCGTCTGGCCGGAGCGTCGGAGTTTCACGGATAAGAATCTTGGGGCGATTCCGAGGCGGTGGAAGGGGGTTTGCGAGACAGGAACTAAATTCGCGAAGAGTAACTGCAACAGGAAACTGATCGGAGCTCGATTCTTCATCAAGGGCCACGAGGCGGCGGCCAATGCCGGAGGTCCGATATCACCGATCAACGACACGGTGGAGTACCGGTCGCCGAGAGATGCGGATGGTCATGGGACCCACACCGCCTCCACGGCGATCGGCCGGTACGCGTTCGAGGCGAGCATGTCGGGTTACGCCGCCGGAATAGCGAAAGGAGTCGCTCCAAAAGCTCGGTTGGCGGTTTATAAGGTATGCTGGAAGGATTCTGGTTGCTTCGACTCCGATATATTGGCTGCCTTTGACGCTGCCGTTAATGACGGCGTTGATGTCATTTCTATTTCAATCGGAGGCGGCGACGGTGTTTCTGCTCCGTATTATCTCGATCCGATTGCGATTGGTTCGTACGGAGCGGTTTCGCACGGAGTGTTCGTGTCTTCCTCGGCCGGCAACGACGGTCCAAACGGCATGTCGGTGACTAATCTCGCGCCTTGGCTTACTACGGTGGGCGCGGGAACCATCGATCGGAATTTCCCGGCGGTTGTGATTTTAGGCAACGGCCGGAGACTAAGCGGTGTGTCGTTGTACTCCGGATTACCTCTCAAAGGCAAGATGTACCCTGTCGTTTATCCTGGGAAATCAAGCATGCTCTCTGCTTCGCTGTGCATGGAGAACACCCTGGACCCGCGCCAGGTCAGGGGCAAAATTGTAATATGCGACCGCGGGAGCAGTCCTCGAGCGGCCAAAGGCTTGGTCGTGAAGAAAGCCGGCGGTGTTGGAATGATCCTCGCTAACGGAATTTCTAACGGCGAGGGTCTCGTCGGCGACGCACATATCCTGGCCACTTGCGCCGTCGGGGCGGACGAGGGTGACGCAATAAAGGCCTACGTGGCCTCCACCAAGACCCCCATGGCGACGCTCGATTTCCAAGGGACTGTGATTGGAATCAAACCGGCTCCGGTTGTGGCTTCGTTCTCGGGTCGTGGGCCGAACGGGCTGAACCCGGAAATTCTGAAGCCAGACCTGATCGCCCCGGGAGTCAACATCTTGGCCGCTTGGACAGACTCCGTGGGACCCACCGGTCTGGACACTGACAAGAGGAAAACGGAGTTTAACATTTTGTCCGGGACTTCGATGGCCGCTCCGCACGTGAGCGGAGCTGCGGCTTTGCTGAAGTCGGCTCACCGGGATTGGAGCCCAGCCGCCATAAGATCCGCCATGATGACGACGGCGAGCGTGACTGATAACAGAAATCAGACCATGACTGATGAGGCAACCGGAAAGCCCTCTACGCCTTATGATTTGGGGGCGGGTCATTTGAATTTGGGCCGGGCTATCGATCCTGGGCTTATTTATGACATCACCAATGATGATTACGTTCGGTTTCTTTGTGGAGTTGGGTATGGGCCAAGAGTGATTCAGGTCATCACTCGGGTCCAGCCCAAATGTCCGGTGAAGAAGCCTGCGCCGGAAAATCTCAATTACCCGTCCATTGGCGCGGTGTTTTCCAGGGCGGGTAAGTCGAGTAAGATGTTTATTCGGACCGTGACGAATGTGGGGCAACCCAATGCGGTGTACGGGTCTAGGATTGAGGCCCCAAAAGGGGTGACAGTGTCAGTGAAGCCGTCGAGGCTGGTGTTTAGTGGGGCGGTAAAGAAGCGGAGCTTTGTAGTGACGGTCGCGGTGGATAGGAGGAATGTGGTGTTTGGTGAGTCAGGTGCGGCGTTCGGGTCGCTTTACTGGAGCGACGGGAAGCACGTGGTTCGGAGCCCCATTGTAGTGACACAAATGGATCCCTTGTAA

Functional Annotation

Gene Ontology

Molecular Function
Biological Process
Cellular Component

No ontology terms assigned.

KEGG Pathways

Metabolic & Signaling

No pathways identified.

Pfam Domains

Protein Families

Inhibitor_I9 PA Peptidase_S8

Protein Analysis

833

Amino Acids

88.37

Weight (kDa)

9.43

Isoelectric Point (pI)

34.76

Instability Index

Protein Domains (Pfam)

Domain Name	Pfam ID	Position	E-value	Description
Inhibitor_I9	PF05922	96 - 166	1.6e-12	Peptidase inhibitor I9
Peptidase_S8	PF00082	189 - 658	3.1e-46	Subtilase family
PA	PF02225	438 - 525	1.4e-11	PA domain
fn3_6	PF17766	724 - 826	8e-29	Fibronectin type-III domain

Hydrophobicity Profile (Kyte-Doolittle)

AI Structure Prediction Report

Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter

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Align Labels (Cladogram)

Image

Tree File

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Publication-ready

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Orthologous Genes (Group: OG0004305)

Species	Orthologous Gene IDs
arabidopsis_thaliana	AT4G34980
fragaria_vesca	FvH4_2g01410 FvH4_3g19440
malus_domestica	MD05G1120100.v1.1 MD10G1123500.v1.1
prunus_persica	Prupe.8G164000_v2.0.a1
pyrus_communis	pycom05g11390 pycom10g10580
rosa_chinensis	RchiOBHm_Chr6g0244021 RchiOBHm_Chr6g0252351 RchiOBHm_Chr6g0252361
rosa_laevigata	RLG00000015390 RLG00000029339
rosa_multiflora	Rmu_co8423929.1_g000001 Rmu_sc0001251.1_g000002 Rmu_sc0006415.1_g000012 Rmu_sc0006711.1_g000006 Rmu_sc0007419.1_g000002
rosa_roxburghii	Rroxscaffold_6G00388820 Rroxscaffold_6G00388840
rosa_rugosa	Rorug05G0223500 Rorug05G0506700 Rorug05G0515900
rosa_samantha	Rh1CG388200 Rh2AG211900 Rh3DG088300 Rh5BG286100 Rh5DG294800 Rh6AG021200 Rh6CG013400 Rh6DG014700
rosa_wichuraiana	Rw6G001740

Restriction Enzyme Sites

1 / 10

Enzyme	Recognition Site	Cut Count	Positions (bp)
AanI	TTATAA	2 cut(s)	107, 945
AasI	GACNNNNNNGTC	3 cut(s)	578, 599, 1776
AatII	GACGTC	1 cut(s)	576
AccBSI	CCGCTC	4 cut(s)	1109, 1436, 1838, 1851
AccI	GTMKAC	3 cut(s)	56, 297, 384
AccIII	TCCGGA	1 cut(s)	1286
AclWI	GGATC	9 cut(s)	367, 466, 501, 1076, 1474, 1902, 2030, 2483, 2496
AcoI	YGGCCR	9 cut(s)	605, 756, 859, 1140, 1255, 1437, 1536, 1746, 1833
AcsI	RAATTY	7 cut(s)	233, 353, 686, 1225, 1494, 1704, 2017
AcuI	CTGAAG	2 cut(s)	1730, 1889
AcvI	CACGTG	2 cut(s)	1846, 2455
AcyI	GRCGYC	2 cut(s)	573, 600
AdeI	CACNNNGTG	1 cut(s)	1123
AfaI	GTAC	7 cut(s)	344, 803, 866, 1103, 1283, 1313, 2273
AflIII	ACRYGT	1 cut(s)	867
AgeI	ACCGGT	2 cut(s)	804, 1775
AgsI	TTSAA	3 cut(s)	232, 1041, 2017
AjiI	CACGTC	1 cut(s)	382
AjnI	CCWGG	8 cut(s)	429, 474, 1327, 1374, 1386, 1533, 2038, 2204
AluBI	AGCT	4 cut(s)	725, 929, 1856, 2361
AluI	AGCT	4 cut(s)	725, 929, 1856, 2361
Alw21I	GWGCWC	1 cut(s)	727
Alw26I	GTCTC	5 cut(s)	439, 497, 668, 1255, 1517
AlwI	GGATC	9 cut(s)	367, 466, 501, 1076, 1474, 1902, 2030, 2483, 2496
Ama87I	CYCGRG	4 cut(s)	1431, 1672, 1729, 2127
Aor13HI	TCCGGA	1 cut(s)	1286
ApeKI	GCWGC	3 cut(s)	992, 1004, 1856
ApoI	RAATTY	7 cut(s)	233, 353, 686, 1225, 1494, 1704, 2017
ArsI	GACNNNNNNTTYG	4 cut(s)	1151, 1183, 1434, 1466
AsiGI	ACCGGT	2 cut(s)	804, 1775
AspLEI	GCGC	8 cut(s)	36, 253, 1186, 1208, 1386, 1547, 2164, 2195
AsuC2I	CCSGG	7 cut(s)	1232, 1700, 1730, 1731, 1822, 1883, 2027
AsuHPI	GGTGA	8 cut(s)	771, 1183, 1577, 1871, 2050, 2161, 2314, 2417
AvaI	CYCGRG	4 cut(s)	1431, 1672, 1729, 2127
AvaII	GGWCC	8 cut(s)	478, 770, 834, 1154, 1378, 1769, 2131, 2238
BalI	TGGCCA	1 cut(s)	1538
BamHI	GGATCC	1 cut(s)	2488
BanII	GRGCYC	4 cut(s)	528, 727, 1895, 2468
BauI	CACGAG	1 cut(s)	746
BbrPI	CACGTG	2 cut(s)	1846, 2455
BbsI	GAAGAC	1 cut(s)	1125
Bbv12I	GWGCWC	1 cut(s)	727
BbvI	GCAGC	3 cut(s)	979, 991, 1843
BccI	CCATC	4 cut(s)	449, 818, 1223, 1825
BcgI	CGANNNNNNTGC	4 cut(s)	1070, 1104, 2441, 2475
BciT130I	CCWGG	8 cut(s)	431, 476, 1329, 1376, 1388, 1535, 2040, 2206
BcnI	CCSGG	7 cut(s)	1232, 1700, 1730, 1731, 1822, 1883, 2027
BcoDI	GTCTC	5 cut(s)	439, 497, 668, 1255, 1517
BfaI	CTAG	1 cut(s)	2280
BglI	GCCNNNNNGGC	5 cut(s)	851, 1553, 1586, 1690, 2326
Bme18I	GGWCC	8 cut(s)	478, 770, 834, 1154, 1378, 1769, 2131, 2238
BmeT110I	CYCGRG	4 cut(s)	1431, 1672, 1729, 2127
BmgBI	CACGTC	1 cut(s)	382
BmsI	GCATC	1 cut(s)	808
BpiI	GAAGAC	1 cut(s)	1125
BplI	GAGNNNNNCTC	2 cut(s)	1414, 1446
BpmI	CTGGAG	1 cut(s)	2458
Bpu10I	CCTNAGC	1 cut(s)	527
BpuEI	CTTGAG	1 cut(s)	260
BpuMI	CCSGG	7 cut(s)	1232, 1700, 1730, 1731, 1822, 1883, 2027
Bsa29I	ATCGAT	2 cut(s)	1218, 2034
BsaAI	YACGTR	3 cut(s)	1585, 1846, 2455
BsaBI	GATNNNNATC	2 cut(s)	633, 1640
BsaHI	GRCGYC	2 cut(s)	573, 600
BsaI	GGTCTC	2 cut(s)	439, 1517
BsaWI	WCCGGW	6 cut(s)	804, 1286, 1654, 1775, 1973, 2146
Bse118I	RCCGGY	7 cut(s)	596, 804, 861, 1142, 1463, 1648, 1775
Bse1I	ACTGG	2 cut(s)	344, 2441
Bse3DI	GCAATG	1 cut(s)	11
Bse8I	GATNNNNATC	2 cut(s)	633, 1640
BseAI	TCCGGA	1 cut(s)	1286
BseBI	CCWGG	8 cut(s)	431, 476, 1329, 1376, 1388, 1535, 2040, 2206
BseCI	ATCGAT	2 cut(s)	1218, 2034
BseGI	GGATG	1 cut(s)	829
BseJI	GATNNNNATC	2 cut(s)	633, 1640
BseMI	GCAATG	1 cut(s)	11
BseMII	CTCAG	2 cut(s)	252, 541
BseNI	ACTGG	2 cut(s)	344, 2441
BseRI	GAGGAG	3 cut(s)	109, 191, 200
BseX3I	CGGCCG	3 cut(s)	859, 1140, 1255
BseXI	GCAGC	3 cut(s)	979, 991, 1843
BseYI	CCCAGC	1 cut(s)	1894
Bsh1285I	CGRYCG	9 cut(s)	301, 809, 858, 862, 1143, 1222, 1258, 1417, 2376
BshTI	ACCGGT	2 cut(s)	804, 1775
BshVI	ATCGAT	2 cut(s)	1218, 2034
BsiEI	CGRYCG	9 cut(s)	301, 809, 858, 862, 1143, 1222, 1258, 1417, 2376
BsiHKAI	GWGCWC	1 cut(s)	727
BsiHKCI	CYCGRG	4 cut(s)	1431, 1672, 1729, 2127
BsiWI	CGTACG	1 cut(s)	1101
BslFI	GGGAC	5 cut(s)	159, 847, 1642, 1782, 1837
BsmAI	GTCTC	5 cut(s)	439, 497, 668, 1255, 1517
BsmFI	GGGAC	5 cut(s)	159, 847, 1642, 1782, 1837
Bso31I	GGTCTC	2 cut(s)	439, 1517
BsoBI	CYCGRG	4 cut(s)	1431, 1672, 1729, 2127
Bsp1286I	GDGCHC	4 cut(s)	528, 727, 1895, 2468
Bsp13I	TCCGGA	1 cut(s)	1286
Bsp19I	CCATGG	1 cut(s)	1605
Bsp68I	TCGCGA	1 cut(s)	692
BspCNI	CTCAG	2 cut(s)	251, 540
BspDI	ATCGAT	2 cut(s)	1218, 2034
BspEI	TCCGGA	1 cut(s)	1286
BspPI	GGATC	9 cut(s)	367, 466, 501, 1076, 1474, 1902, 2030, 2483, 2496
BspTNI	GGTCTC	2 cut(s)	439, 1517
BsrBI	CCGCTC	4 cut(s)	1109, 1436, 1838, 1851
BsrDI	GCAATG	1 cut(s)	11
BsrFI	RCCGGY	7 cut(s)	596, 804, 861, 1142, 1463, 1648, 1775
BsrI	ACTGG	2 cut(s)	344, 2441
BssAI	RCCGGY	7 cut(s)	596, 804, 861, 1142, 1463, 1648, 1775
BssNI	GRCGYC	2 cut(s)	573, 600
BssSI	CACGAG	1 cut(s)	746
BssT1I	CCWWGG	3 cut(s)	1187, 1605, 1624
Bst2BI	CACGAG	1 cut(s)	746
Bst2UI	CCWGG	8 cut(s)	431, 476, 1329, 1376, 1388, 1535, 2040, 2206
Bst6I	CTCTTC	1 cut(s)	689
BstACI	GRCGYC	2 cut(s)	573, 600
BstBAI	YACGTR	3 cut(s)	1585, 1846, 2455
BstC8I	GCNNGC	8 cut(s)	258, 465, 880, 1144, 1343, 1465, 1930, 2160
BstDEI	CTNAG	3 cut(s)	238, 527, 1265
BstDSI	CCRYGG	5 cut(s)	400, 849, 1417, 1605, 1764
BstENI	CCTNNNNNAGG	1 cut(s)	1299
BstF5I	GGATG	1 cut(s)	829
BstHHI	GCGC	8 cut(s)	36, 253, 1186, 1208, 1386, 1547, 2164, 2195
BstMAI	GTCTC	5 cut(s)	439, 497, 668, 1255, 1517
BstMCI	CGRYCG	9 cut(s)	301, 809, 858, 862, 1143, 1222, 1258, 1417, 2376
BstNI	CCWGG	8 cut(s)	431, 476, 1329, 1376, 1388, 1535, 2040, 2206
BstNSI	RCATGY	3 cut(s)	886, 1168, 1345
BstV1I	GCAGC	3 cut(s)	979, 991, 1843
BstV2I	GAAGAC	1 cut(s)	1125
BstX2I	RGATCY	3 cut(s)	372, 1907, 2488
BstXI	CCANNNNNNTGG	2 cut(s)	1447, 1780
BstYI	RGATCY	3 cut(s)	372, 1907, 2488
BstZI	CGGCCG	3 cut(s)	859, 1140, 1255
Bsu15I	ATCGAT	2 cut(s)	1218, 2034
BsuTUI	ATCGAT	2 cut(s)	1218, 2034
BtgI	CCRYGG	5 cut(s)	400, 849, 1417, 1605, 1764
BtrI	CACGTC	1 cut(s)	382
BtsCI	GGATG	1 cut(s)	829
BtsIMutI	CAGTG	4 cut(s)	337, 1785, 2313, 2319
BtuMI	TCGCGA	1 cut(s)	692
Cac8I	GCNNGC	8 cut(s)	258, 465, 880, 1144, 1343, 1465, 1930, 2160
CfoI	GCGC	8 cut(s)	36, 253, 1186, 1208, 1386, 1547, 2164, 2195
Cfr10I	RCCGGY	7 cut(s)	596, 804, 861, 1142, 1463, 1648, 1775
Cfr42I	CCGCGG	1 cut(s)	1420
Cfr9I	CCCGGG	1 cut(s)	1729
ClaI	ATCGAT	2 cut(s)	1218, 2034
CpoI	CGGWCCG	1 cut(s)	2238
CseI	GACGC	7 cut(s)	283, 589, 602, 1010, 1532, 1577, 1621
CsiI	ACCWGGT	1 cut(s)	429
Csp6I	GTAC	7 cut(s)	343, 802, 865, 1102, 1282, 1312, 2272
CspAI	ACCGGT	2 cut(s)	804, 1775
CspCI	CAANNNNNGTGG	2 cut(s)	501, 536
CspI	CGGWCCG	1 cut(s)	2238
CviAII	CATG	8 cut(s)	830, 883, 1165, 1342, 1363, 1606, 1915, 1957
CviQI	GTAC	7 cut(s)	343, 802, 865, 1102, 1282, 1312, 2272
DdeI	CTNAG	3 cut(s)	238, 527, 1265
DraIII	CACNNNGTG	1 cut(s)	1123
DrdI	GACNNNNNNGTC	3 cut(s)	578, 599, 1776
DseDI	GACNNNNNNGTC	3 cut(s)	578, 599, 1776
EaeI	YGGCCR	9 cut(s)	605, 756, 859, 1140, 1255, 1437, 1536, 1746, 1833
EagI	CGGCCG	3 cut(s)	859, 1140, 1255
Eam1104I	CTCTTC	1 cut(s)	689
EarI	CTCTTC	1 cut(s)	689
EciI	GGCGGA	4 cut(s)	400, 427, 1571, 1900
Ecl136II	GAGCTC	1 cut(s)	725
EclXI	CGGCCG	3 cut(s)	859, 1140, 1255
Eco130I	CCWWGG	3 cut(s)	1187, 1605, 1624
Eco147I	AGGCCT	1 cut(s)	1580
Eco24I	GRGCYC	4 cut(s)	528, 727, 1895, 2468
Eco31I	GGTCTC	2 cut(s)	439, 1517
Eco32I	GATATC	1 cut(s)	777
Eco47I	GGWCC	8 cut(s)	478, 770, 834, 1154, 1378, 1769, 2131, 2238
Eco52I	CGGCCG	3 cut(s)	859, 1140, 1255
Eco53kI	GAGCTC	1 cut(s)	725
Eco57I	CTGAAG	2 cut(s)	1730, 1889
Eco72I	CACGTG	2 cut(s)	1846, 2455
Eco88I	CYCGRG	4 cut(s)	1431, 1672, 1729, 2127
EcoICRI	GAGCTC	1 cut(s)	725
EcoNI	CCTNNNNNAGG	1 cut(s)	1299
EcoO109I	RGGNCCY	4 cut(s)	28, 834, 1769, 2290
EcoRI	GAATTC	1 cut(s)	353
EcoRII	CCWGG	8 cut(s)	429, 474, 1327, 1374, 1386, 1533, 2038, 2204
EcoRV	GATATC	1 cut(s)	777
EcoT14I	CCWWGG	3 cut(s)	1187, 1605, 1624
EcoT38I	GRGCYC	4 cut(s)	528, 727, 1895, 2468
ErhI	CCWWGG	3 cut(s)	1187, 1605, 1624
FaeI	CATG	8 cut(s)	833, 886, 1168, 1345, 1366, 1609, 1918, 1960
FalI	AAGNNNNNCTT	2 cut(s)	2345, 2377
FaqI	GGGAC	5 cut(s)	159, 847, 1642, 1782, 1837
FatI	CATG	8 cut(s)	829, 882, 1164, 1341, 1362, 1605, 1914, 1956
FauI	CCCGC	7 cut(s)	39, 372, 1201, 1389, 1412, 1999, 2203
FblI	GTMKAC	3 cut(s)	56, 297, 384
FokI	GGATG	1 cut(s)	836
FriOI	GRGCYC	4 cut(s)	528, 727, 1895, 2468
FspBI	CTAG	1 cut(s)	2280
GlaI	GCGC	8 cut(s)	35, 252, 1185, 1207, 1385, 1546, 2163, 2194
GsaI	CCCAGC	1 cut(s)	1898
GsuI	CTGGAG	1 cut(s)	2458
HgaI	GACGC	7 cut(s)	283, 589, 602, 1010, 1532, 1577, 1621
HhaI	GCGC	8 cut(s)	36, 253, 1186, 1208, 1386, 1547, 2164, 2195
Hin1I	GRCGYC	2 cut(s)	573, 600
Hin1II	CATG	8 cut(s)	833, 886, 1168, 1345, 1366, 1609, 1918, 1960
Hin6I	GCGC	8 cut(s)	34, 251, 1184, 1206, 1384, 1545, 2162, 2193
HinP1I	GCGC	8 cut(s)	34, 251, 1184, 1206, 1384, 1545, 2162, 2193
HincII	GTYRAC	2 cut(s)	298, 1738
HindII	GTYRAC	2 cut(s)	298, 1738
HphI	GGTGA	8 cut(s)	771, 1183, 1577, 1871, 2050, 2161, 2314, 2417
Hpy166II	GTNNAC	5 cut(s)	57, 298, 385, 1738, 2272
Hpy188III	TCNNGA	8 cut(s)	277, 425, 529, 691, 1079, 1287, 1453, 1781
Hpy8I	GTNNAC	5 cut(s)	57, 298, 385, 1738, 2272
HpyAV	CCTTC	5 cut(s)	188, 292, 465, 655, 952
HpyCH4IV	ACGT	6 cut(s)	381, 573, 1584, 1845, 2073, 2454
HpyCH4V	TGCA	3 cut(s)	4, 705, 1362
HpyF3I	CTNAG	3 cut(s)	238, 527, 1265
HpySE526I	ACGT	6 cut(s)	381, 573, 1584, 1845, 2073, 2454
Hsp92I	GRCGYC	2 cut(s)	573, 600
Hsp92II	CATG	8 cut(s)	833, 886, 1168, 1345, 1366, 1609, 1918, 1960
HspAI	GCGC	8 cut(s)	34, 251, 1184, 1206, 1384, 1545, 2162, 2193
KflI	GGGWCCC	2 cut(s)	834, 1769
Kpn2I	TCCGGA	1 cut(s)	1286
KroI	GCCGGC	2 cut(s)	1142, 1463
KroNI	GCCGGC	2 cut(s)	1144, 1465
KspI	CCGCGG	1 cut(s)	1420
Lsp1109I	GCAGC	3 cut(s)	979, 991, 1843
LweI	GCATC	1 cut(s)	808
MabI	ACCWGGT	1 cut(s)	429
MaeI	CTAG	1 cut(s)	2280
MaeII	ACGT	6 cut(s)	381, 573, 1584, 1845, 2073, 2454
MbiI	CCGCTC	4 cut(s)	1109, 1436, 1838, 1851
MboII	GAAGA	5 cut(s)	706, 724, 1125, 1468, 2164
MflI	RGATCY	3 cut(s)	372, 1907, 2488
MhlI	GDGCHC	4 cut(s)	528, 727, 1895, 2468
MlsI	TGGCCA	1 cut(s)	1538
MluI	ACGCGT	1 cut(s)	867
MluNI	TGGCCA	1 cut(s)	1538
MlyI	GAGTC	6 cut(s)	44, 924, 971, 1743, 1754, 2417
MmeI	TCCRAC	6 cut(s)	471, 531, 562, 594, 596, 1453
Mox20I	TGGCCA	1 cut(s)	1538
MroI	TCCGGA	1 cut(s)	1286
MroNI	GCCGGC	2 cut(s)	1142, 1463
MscI	TGGCCA	1 cut(s)	1538
MseI	TTAA	2 cut(s)	1011, 1809
MslI	CAYNNNNRTG	2 cut(s)	1169, 2474
Msp20I	TGGCCA	1 cut(s)	1538
MspA1I	CMGCKG	1 cut(s)	1419
MvaI	CCWGG	8 cut(s)	431, 476, 1329, 1376, 1388, 1535, 2040, 2206
NaeI	GCCGGC	2 cut(s)	1144, 1465
NciI	CCSGG	7 cut(s)	1232, 1700, 1730, 1731, 1822, 1883, 2027
NcoI	CCATGG	1 cut(s)	1605
NgoMIV	GCCGGC	2 cut(s)	1142, 1463
NlaIII	CATG	8 cut(s)	833, 886, 1168, 1345, 1366, 1609, 1918, 1960
NmeAIII	GCCGAG	2 cut(s)	837, 1118
NmuCI	GTSAC	8 cut(s)	133, 1171, 1565, 1933, 2242, 2302, 2368, 2476
NruI	TCGCGA	1 cut(s)	692
NspI	RCATGY	3 cut(s)	886, 1168, 1345
PaeI	GCATGC	1 cut(s)	1345
PaeR7I	CTCGAG	1 cut(s)	1431
PceI	AGGCCT	1 cut(s)	1580
PcsI	WCGNNNNNNNCGW	4 cut(s)	1050, 1125, 1557, 2451
PdiI	GCCGGC	2 cut(s)	1144, 1465
PfeI	GAWTC	8 cut(s)	12, 554, 634, 646, 729, 962, 1641, 2113
Pfl23II	CGTACG	1 cut(s)	1101
PflFI	GACNNNGTC	2 cut(s)	431, 2308
PfoI	TCCNGGA	2 cut(s)	474, 1820
PinAI	ACCGGT	2 cut(s)	804, 1775
Ple19I	CGATCG	2 cut(s)	858, 1222
PleI	GAGTC	6 cut(s)	44, 923, 971, 1742, 1754, 2416
PmaCI	CACGTG	2 cut(s)	1846, 2455
PmlI	CACGTG	2 cut(s)	1846, 2455
PpsI	GAGTC	6 cut(s)	44, 923, 971, 1742, 1754, 2416
Ppu21I	YACGTR	3 cut(s)	1585, 1846, 2455
PpuMI	RGGWCCY	2 cut(s)	834, 1769
PsiI	TTATAA	2 cut(s)	107, 945
Psp124BI	GAGCTC	1 cut(s)	727
Psp5II	RGGWCCY	2 cut(s)	834, 1769
Psp6I	CCWGG	8 cut(s)	429, 474, 1327, 1374, 1386, 1533, 2038, 2204
PspCI	CACGTG	2 cut(s)	1846, 2455
PspFI	CCCAGC	1 cut(s)	1894
PspGI	CCWGG	8 cut(s)	429, 474, 1327, 1374, 1386, 1533, 2038, 2204
PspLI	CGTACG	1 cut(s)	1101
PspPPI	RGGWCCY	2 cut(s)	834, 1769
PspXI	VCTCGAGB	1 cut(s)	1431
PsuI	RGATCY	3 cut(s)	372, 1907, 2488
PsyI	GACNNNGTC	2 cut(s)	431, 2308
PvuI	CGATCG	2 cut(s)	858, 1222
RruI	TCGCGA	1 cut(s)	692
RsaI	GTAC	7 cut(s)	344, 803, 866, 1103, 1283, 1313, 2273
RsaNI	GTAC	7 cut(s)	343, 802, 865, 1102, 1282, 1312, 2272
RseI	CAYNNNNRTG	2 cut(s)	1169, 2474
Rsr2I	CGGWCCG	1 cut(s)	2238
RsrII	CGGWCCG	1 cut(s)	2238
SacI	GAGCTC	1 cut(s)	727
SacII	CCGCGG	1 cut(s)	1420
SalI	GTCGAC	1 cut(s)	296
SaqAI	TTAA	2 cut(s)	1011, 1809
SchI	GAGTC	6 cut(s)	44, 924, 971, 1743, 1754, 2417
SduI	GDGCHC	4 cut(s)	528, 727, 1895, 2468
SexAI	ACCWGGT	1 cut(s)	429
SfaNI	GCATC	1 cut(s)	808
SfiI	GGCCNNNNNGGCC	1 cut(s)	1586
Sfr274I	CTCGAG	1 cut(s)	1431
Sfr303I	CCGCGG	1 cut(s)	1420
SgrAI	CRCCGGYG	1 cut(s)	596
SgrBI	CCGCGG	1 cut(s)	1420
SinI	GGWCC	8 cut(s)	478, 770, 834, 1154, 1378, 1769, 2131, 2238
SlaI	CTCGAG	1 cut(s)	1431
SmaI	CCCGGG	1 cut(s)	1731
SmiMI	CAYNNNNRTG	2 cut(s)	1169, 2474
SmlI	CTYRAG	2 cut(s)	275, 1431
SmoI	CTYRAG	2 cut(s)	275, 1431
SphI	GCATGC	1 cut(s)	1345
SseBI	AGGCCT	1 cut(s)	1580
SspMI	CTAG	1 cut(s)	2280
SstI	GAGCTC	1 cut(s)	727
StuI	AGGCCT	1 cut(s)	1580
StyI	CCWWGG	3 cut(s)	1187, 1605, 1624
TaiI	ACGT	6 cut(s)	384, 576, 1587, 1848, 2076, 2457
TaqII	GACCGA	1 cut(s)	121
TatI	WGTACW	1 cut(s)	1281
TfiI	GAWTC	8 cut(s)	12, 554, 634, 646, 729, 962, 1641, 2113
Tru1I	TTAA	2 cut(s)	1011, 1809
Tru9I	TTAA	2 cut(s)	1011, 1809
TscAI	CASTG	4 cut(s)	344, 1792, 2313, 2319
TseFI	GTSAC	8 cut(s)	133, 1171, 1565, 1933, 2242, 2302, 2368, 2476
TseI	GCWGC	3 cut(s)	992, 1004, 1856
Tsp45I	GTSAC	8 cut(s)	133, 1171, 1565, 1933, 2242, 2302, 2368, 2476
TspDTI	ATGAA	2 cut(s)	274, 724
TspGWI	ACGGA	7 cut(s)	641, 1059, 1119, 1134, 1506, 1753, 1817
TspMI	CCCGGG	1 cut(s)	1729
TspRI	CASTG	4 cut(s)	344, 1792, 2313, 2319
Tth111I	GACNNNGTC	2 cut(s)	431, 2308
VpaK11BI	GGWCC	8 cut(s)	478, 770, 834, 1154, 1378, 1769, 2131, 2238
XagI	CCTNNNNNAGG	1 cut(s)	1299
XapI	RAATTY	7 cut(s)	233, 353, 686, 1225, 1494, 1704, 2017
XceI	RCATGY	3 cut(s)	886, 1168, 1345
XcmI	CCANNNNNNNNNTGG	1 cut(s)	1603
XhoI	CTCGAG	1 cut(s)	1431
XmaI	CCCGGG	1 cut(s)	1729
XmiI	GTMKAC	3 cut(s)	56, 297, 384
XspI	CTAG	1 cut(s)	2280
ZraI	GACGTC	1 cut(s)	574

Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.