pycom10g10800

Arabidopsis phospholipase-like protein (PEARLI 4)

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
pyrus_communis
Chr10
OG0002091
Physical Location & Seq
Reverse (-)
14247001 .. 14251162
4162 bp
Loading structure...
UTR
Exon/CDS
Intron
N/A

Sequence Viewer

Length: 1719 bp
ATGTCAGTAGGCCTGTCCTTTCTGCTGCTCTTCACACATAAGCCGCCTCCTTTTGTCCTGTTGAATCGCCTGCGGCATCAAAAACAGAAAACCCAATTGAATTCAAGGGTTTGCAGGGTTGTTGGTGATCTCAGTGGAGAGATGTCTCTGATTGGAAGGGTACACCCCGAATGTGTAAACGTAGCCAATCCCTACCATGAATGCACTGAAAACTGCTTGAGAAAGATTGCTGAAGGGAAAGGCCGGAAGTTGACGAAAAAGAAATCAGATTTTGGCAGCAGTTTTAAAGATGTTGAGTACAAAAAAAAGGGGGCTGAAGACAAAAGAGCATCTAATCCTTACCATGACCCTGAATTCTACCGCCAAAGAGCCGCTGCTGCCGAACCTCGTGGGGACAAAAAAGAATCAGGTTCACTACTTGATATTCCTATATTGGGCAGAAAAAAGGAGAGATCCCGACCAAACACCCCTCAGGAGCTTGACCGAAGTGTGATTTATCATTTAGATGCTCCGTCTTCTCCGTCCAAGGAAAAGGTGAAATTAGAGAACGGCTCTGTCCTTGATGATCCTATATTCGGCAGAAGAAAGCAGGGATCTCAGCCAAAGCCCTCTGAAGTGCTTGACAATGTAACTGGTCGAGGTGTAATTTATCCCTCTGATGCTCCATCTAAAGAAATTGTGCAAGTAGAGAATGGCTCCGCACTTGATGATCCTATATTTGGCGGACGAAGGCCTGGTTCCAGACCAAAGCGCCCTGAAGAGCGTGACAATGTACCTGATGAAATTAAGGAAAAGGTTAAAATAGGAAATGGTGAGCATAAATCTTATTCTCCACCGGCTGCTGAAGTAAAAGATGCTACCTCCAACAAAGAGCAAGTTCCGTCCTCTCTGTTAGTGCCACCATCGGGAATTCTCACAATGCCTGAAAATCCCAAAAACCCTCGAGAGAATGATGCTACTTATGTTGCTAGTGAACGACCAGGTCATGGGGAAGACAATGAAAATATTCATTCCCCAGGAGTTGATTCAAATCGTACTGTCAACAATGTAGAAGGCGCTACTGGATCAGCTTACGGCTCAGGCTTTAGTTTCTCGGGTACTCTCCATGCTTTTGAAGATAGTGATGATGAAGATACACGATCGGTTAATTCTGAGTCCAGTGTCTCTGTGGGAAAATACCGTGTGAAAGGAAATTTCTCTTCCATTCTCCAGTCAATCATGGAAAAATATGGTGACATAGCCGCAAGCTGTCAATTGGAATCAGTTGTCATGCGCTCTTACTATCTCGAGTGCGTATGTTATATAGTCCAAGAGTTGAAGCGAACTTCGGTTAAGCAGCTGACAAAGACTAAAGTCAAAGAAATGTTATCTATGCTTAAGGATGTAGAATCTTCAGGAATGGATGTTGGTTGGCTCCGATTTCTGATCAACGAGTGTGCAGAAGCCATTGAACTCGTCACTCAGCATCGAGCATTCGAAGCAGCAAAGGCAAACTGCGACCGTGATATAGAATCAGCAAGGAAAGAACTTGAATCGCAAATGGAGGTTCTGGCACTGAAAGAAAAGGAGGTTGCCAATGCCAAGAAGCAAGTCGCTGAAACGAGAGCCCACCTGAGGGAGCTCGAGCTTAAATCTTCCGTCTTCACTGAGAATATCAGTAACATAAAGTCCAAGGTCGAAAATCTTCCAAGCAAACCTTTGTTGGACAAAGTCTTGTGA
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
No pathways identified.
Pfam Domains
Protein Families
PEARLI-4

Protein Analysis

573

Amino Acids

63.54

Weight (kDa)

8.18

Isoelectric Point (pI)

49.64

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
PEARLI-4 PF05278 343 - 569 1.3e-63 Arabidopsis phospholipase-like protein (PEARLI 4)
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Publication-ready
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Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AccB7I CCANNNNNTGG 1 cut(s) 986
AciI CCGC 7 cut(s) 44, 73, 361, 372, 699, 723, 1242
AclWI GGATC 5 cut(s) 447, 560, 601, 704, 1072
AcsI RAATTY 4 cut(s) 100, 353, 909, 1192
AcuI CTGAAG 6 cut(s) 252, 336, 633, 777, 864, 1377
AfaI GTAC 5 cut(s) 162, 299, 774, 1036, 1099
AfiI CCNNNNNNNGG 7 cut(s) 434, 575, 719, 905, 986, 1614, 1615
AflII CTTAAG 1 cut(s) 1376
AgsI TTSAA 8 cut(s) 64, 100, 105, 1029, 1115, 1318, 1451, 1532
AjnI CCWGG 3 cut(s) 733, 979, 1015
AluBI AGCT 6 cut(s) 478, 1070, 1248, 1339, 1621, 1627
AluI AGCT 6 cut(s) 478, 1070, 1248, 1339, 1621, 1627
Alw21I GWGCWC 1 cut(s) 1623
Alw26I GTCTC 2 cut(s) 150, 1168
AlwI GGATC 5 cut(s) 447, 560, 601, 704, 1072
Ama87I CYCGRG 4 cut(s) 942, 1093, 1286, 1622
AoxI GGCC 3 cut(s) 10, 241, 731
ApeKI GCWGC 7 cut(s) 25, 276, 374, 377, 839, 1336, 1481
ApoI RAATTY 4 cut(s) 100, 353, 909, 1192
Asp700I GAANNNNTTC 2 cut(s) 1005, 1683
AspLEI GCGC 3 cut(s) 753, 1058, 1275
AsuHPI GGTGA 4 cut(s) 137, 547, 824, 1244
AsuII TTCGAA 1 cut(s) 1476
AvaI CYCGRG 4 cut(s) 942, 1093, 1286, 1622
AxyI CCTNAGG 2 cut(s) 471, 1613
BanII GRGCYC 2 cut(s) 1609, 1623
BauI CACGAG 1 cut(s) 387
BbsI GAAGAC 4 cut(s) 324, 507, 999, 1633
Bbv12I GWGCWC 1 cut(s) 1623
BbvI GCAGC 7 cut(s) 12, 288, 361, 364, 826, 1348, 1493
BccI CCATC 2 cut(s) 673, 910
BceAI ACGGC 2 cut(s) 565, 1090
BciT130I CCWGG 3 cut(s) 735, 981, 1017
BclI TGATCA 1 cut(s) 1425
BcoDI GTCTC 2 cut(s) 150, 1168
BfaI CTAG 1 cut(s) 969
BfoI RGCGCY 2 cut(s) 754, 1059
BfrI CTTAAG 1 cut(s) 1376
Bme1390I CCNGG 3 cut(s) 735, 981, 1017
BmeT110I CYCGRG 4 cut(s) 942, 1093, 1286, 1622
BmiI GGNNCC 3 cut(s) 697, 739, 1415
BmrFI CCNGG 3 cut(s) 735, 981, 1017
BmsI GCATC 7 cut(s) 85, 338, 496, 649, 844, 943, 1474
BoxI GACNNNNGTC 1 cut(s) 1352
BpiI GAAGAC 4 cut(s) 324, 507, 999, 1633
BplI GAGNNNNNCTC 4 cut(s) 536, 568, 680, 712
BpmI CTGGAG 1 cut(s) 1193
Bpu10I CCTNAGC 1 cut(s) 1078
Bpu14I TTCGAA 1 cut(s) 1476
BpuEI CTTGAG 1 cut(s) 238
BsaBI GATNNNNATC 1 cut(s) 1029
BsaJI CCNNGG 3 cut(s) 525, 1015, 1671
Bsc4I CCNNNNNNNGG 7 cut(s) 434, 575, 719, 905, 986, 1614, 1615
Bse118I RCCGGY 1 cut(s) 835
Bse1I ACTGG 4 cut(s) 637, 1066, 1158, 1210
Bse21I CCTNAGG 2 cut(s) 471, 1613
Bse8I GATNNNNATC 1 cut(s) 1029
BseBI CCWGG 3 cut(s) 735, 981, 1017
BseDI CCNNGG 3 cut(s) 525, 1015, 1671
BseGI GGATG 2 cut(s) 1387, 1408
BseJI GATNNNNATC 1 cut(s) 1029
BseLI CCNNNNNNNGG 7 cut(s) 434, 575, 719, 905, 986, 1614, 1615
BseMII CTCAG 8 cut(s) 145, 485, 611, 1092, 1143, 1475, 1604, 1638
BseNI ACTGG 4 cut(s) 637, 1066, 1158, 1210
BseXI GCAGC 7 cut(s) 12, 288, 361, 364, 826, 1348, 1493
BsgI GTGCAG 1 cut(s) 1458
Bsh1285I CGRYCG 2 cut(s) 1142, 1501
BshFI GGCC 3 cut(s) 12, 243, 733
BsiEI CGRYCG 2 cut(s) 1142, 1501
BsiHKAI GWGCWC 1 cut(s) 1623
BsiHKCI CYCGRG 4 cut(s) 942, 1093, 1286, 1622
BsiSI CCGG 2 cut(s) 244, 836
BslFI GGGAC 1 cut(s) 407
BslI CCNNNNNNNGG 7 cut(s) 434, 575, 719, 905, 986, 1614, 1615
BsmAI GTCTC 2 cut(s) 150, 1168
BsmFI GGGAC 1 cut(s) 407
BsmI GAATGC 2 cut(s) 206, 1472
BsnI GGCC 3 cut(s) 12, 243, 733
BsoBI CYCGRG 4 cut(s) 942, 1093, 1286, 1622
Bsp119I TTCGAA 1 cut(s) 1476
Bsp1286I GDGCHC 2 cut(s) 1609, 1623
Bsp143I GATC 8 cut(s) 127, 452, 565, 593, 709, 1064, 1139, 1425
BspACI CCGC 7 cut(s) 44, 73, 361, 372, 699, 723, 1242
BspANI GGCC 3 cut(s) 12, 243, 733
BspCNI CTCAG 8 cut(s) 144, 484, 610, 1091, 1144, 1474, 1605, 1639
BspLI GGNNCC 3 cut(s) 697, 739, 1415
BspPI GGATC 5 cut(s) 447, 560, 601, 704, 1072
BspQI GCTCTTC 2 cut(s) 35, 753
BspT104I TTCGAA 1 cut(s) 1476
BspTI CTTAAG 1 cut(s) 1376
BsrFI RCCGGY 1 cut(s) 835
BsrI ACTGG 4 cut(s) 637, 1066, 1158, 1210
BssAI RCCGGY 1 cut(s) 835
BssECI CCNNGG 3 cut(s) 525, 1015, 1671
BssMI GATC 8 cut(s) 127, 452, 565, 593, 709, 1064, 1139, 1425
BssSI CACGAG 1 cut(s) 387
BssT1I CCWWGG 2 cut(s) 525, 1671
Bst2BI CACGAG 1 cut(s) 387
Bst2UI CCWGG 3 cut(s) 735, 981, 1017
Bst4CI ACNGT 3 cut(s) 1039, 1181, 1502
Bst6I CTCTTC 3 cut(s) 35, 753, 1204
BstAFI CTTAAG 1 cut(s) 1376
BstBI TTCGAA 1 cut(s) 1476
BstC8I GCNNGC 2 cut(s) 71, 1246
BstDEI CTNAG 8 cut(s) 131, 471, 597, 1078, 1152, 1461, 1613, 1647
BstF5I GGATG 2 cut(s) 1387, 1408
BstH2I RGCGCY 2 cut(s) 754, 1059
BstHHI GCGC 3 cut(s) 753, 1058, 1275
BstKTI GATC 8 cut(s) 130, 455, 568, 596, 712, 1067, 1142, 1428
BstMAI GTCTC 2 cut(s) 150, 1168
BstMBI GATC 8 cut(s) 127, 452, 565, 593, 709, 1064, 1139, 1425
BstMCI CGRYCG 2 cut(s) 1142, 1501
BstMWI GCNNNNNNNGC 3 cut(s) 377, 1478, 1487
BstNI CCWGG 3 cut(s) 735, 981, 1017
BstPAI GACNNNNGTC 1 cut(s) 1352
BstSCI CCNGG 3 cut(s) 733, 979, 1015
BstV1I GCAGC 7 cut(s) 12, 288, 361, 364, 826, 1348, 1493
BstV2I GAAGAC 4 cut(s) 324, 507, 999, 1633
BstX2I RGATCY 2 cut(s) 452, 593
BstYI RGATCY 2 cut(s) 452, 593
Bsu36I CCTNAGG 2 cut(s) 471, 1613
BsuRI GGCC 3 cut(s) 12, 243, 733
BtsCI GGATG 2 cut(s) 1387, 1408
BtsIMutI CAGTG 5 cut(s) 139, 204, 1165, 1553, 1644
Cac8I GCNNGC 2 cut(s) 71, 1246
CfoI GCGC 3 cut(s) 753, 1058, 1275
Cfr10I RCCGGY 1 cut(s) 835
CsiI ACCWGGT 1 cut(s) 979
Csp6I GTAC 5 cut(s) 161, 298, 773, 1035, 1098
CviAII CATG 6 cut(s) 197, 344, 986, 1106, 1219, 1270
CviQI GTAC 5 cut(s) 161, 298, 773, 1035, 1098
DdeI CTNAG 8 cut(s) 131, 471, 597, 1078, 1152, 1461, 1613, 1647
DpnI GATC 8 cut(s) 129, 454, 567, 595, 711, 1066, 1141, 1427
DpnII GATC 8 cut(s) 127, 452, 565, 593, 709, 1064, 1139, 1425
DraI TTTAAA 1 cut(s) 286
Eam1104I CTCTTC 3 cut(s) 35, 753, 1204
EarI CTCTTC 3 cut(s) 35, 753, 1204
EciI GGCGGA 1 cut(s) 738
Ecl136II GAGCTC 1 cut(s) 1621
Eco130I CCWWGG 2 cut(s) 525, 1671
Eco147I AGGCCT 2 cut(s) 12, 733
Eco24I GRGCYC 2 cut(s) 1609, 1623
Eco53kI GAGCTC 1 cut(s) 1621
Eco57I CTGAAG 6 cut(s) 252, 336, 633, 777, 864, 1377
Eco81I CCTNAGG 2 cut(s) 471, 1613
Eco88I CYCGRG 4 cut(s) 942, 1093, 1286, 1622
EcoICRI GAGCTC 1 cut(s) 1621
EcoRI GAATTC 3 cut(s) 100, 353, 909
EcoRII CCWGG 3 cut(s) 733, 979, 1015
EcoT14I CCWWGG 2 cut(s) 525, 1671
EcoT38I GRGCYC 2 cut(s) 1609, 1623
ErhI CCWWGG 2 cut(s) 525, 1671
FaeI CATG 6 cut(s) 200, 347, 989, 1109, 1222, 1273
FalI AAGNNNNNCTT 2 cut(s) 1681, 1713
FaqI GGGAC 1 cut(s) 407
FatI CATG 6 cut(s) 196, 343, 985, 1105, 1218, 1269
FbaI TGATCA 1 cut(s) 1425
FokI GGATG 2 cut(s) 1394, 1415
FriOI GRGCYC 2 cut(s) 1609, 1623
FspBI CTAG 1 cut(s) 969
GlaI GCGC 3 cut(s) 752, 1057, 1274
GsuI CTGGAG 1 cut(s) 1193
HaeII RGCGCY 2 cut(s) 754, 1059
HaeIII GGCC 3 cut(s) 12, 243, 733
HapII CCGG 2 cut(s) 244, 836
HhaI GCGC 3 cut(s) 753, 1058, 1275
Hin1II CATG 6 cut(s) 200, 347, 989, 1109, 1222, 1273
Hin6I GCGC 3 cut(s) 751, 1056, 1273
HinP1I GCGC 3 cut(s) 751, 1056, 1273
HincII GTYRAC 2 cut(s) 252, 1042
HindII GTYRAC 2 cut(s) 252, 1042
HinfI GANTC 8 cut(s) 64, 404, 1025, 1154, 1259, 1388, 1511, 1532
HpaII CCGG 2 cut(s) 244, 836
HphI GGTGA 4 cut(s) 137, 547, 824, 1244
Hpy166II GTNNAC 6 cut(s) 163, 178, 252, 413, 974, 1042
Hpy188I TCNGA 7 cut(s) 150, 268, 613, 658, 1153, 1418, 1425
Hpy188III TCNNGA 7 cut(s) 456, 473, 741, 906, 944, 1286, 1395
Hpy8I GTNNAC 6 cut(s) 163, 178, 252, 413, 974, 1042
HpyAV CCTTC 4 cut(s) 150, 227, 723, 1046
HpyCH4III ACNGT 3 cut(s) 1039, 1181, 1502
HpyCH4IV ACGT 1 cut(s) 180
HpyCH4V TGCA 4 cut(s) 114, 204, 682, 1439
HpyF10VI GCNNNNNNNGC 3 cut(s) 377, 1478, 1487
HpyF3I CTNAG 8 cut(s) 131, 471, 597, 1078, 1152, 1461, 1613, 1647
HpySE526I ACGT 1 cut(s) 180
Hsp92II CATG 6 cut(s) 200, 347, 989, 1109, 1222, 1273
HspAI GCGC 3 cut(s) 751, 1056, 1273
Ksp22I TGATCA 1 cut(s) 1425
Kzo9I GATC 8 cut(s) 127, 452, 565, 593, 709, 1064, 1139, 1425
LguI GCTCTTC 2 cut(s) 35, 753
LmnI GCTCC 6 cut(s) 475, 514, 667, 701, 1419, 1618
Lsp1109I GCAGC 7 cut(s) 12, 288, 361, 364, 826, 1348, 1493
LweI GCATC 7 cut(s) 85, 338, 496, 649, 844, 943, 1474
MabI ACCWGGT 1 cut(s) 979
MaeI CTAG 1 cut(s) 969
MaeII ACGT 1 cut(s) 180
MaeIII GTNAC 5 cut(s) 628, 764, 1232, 1456, 1658
MalI GATC 8 cut(s) 129, 454, 567, 595, 711, 1066, 1141, 1427
MboI GATC 8 cut(s) 127, 452, 565, 593, 709, 1064, 1139, 1425
MfeI CAATTG 2 cut(s) 95, 1253
MflI RGATCY 2 cut(s) 452, 593
MhlI GDGCHC 2 cut(s) 1609, 1623
MlyI GAGTC 1 cut(s) 1163
MmeI TCCRAC 2 cut(s) 888, 1683
MroXI GAANNNNTTC 2 cut(s) 1005, 1683
MseI TTAA 7 cut(s) 285, 786, 798, 1146, 1332, 1377, 1629
MslI CAYNNNNRTG 1 cut(s) 504
MspA1I CMGCKG 2 cut(s) 374, 1339
MspCI CTTAAG 1 cut(s) 1376
MspI CCGG 2 cut(s) 244, 836
MspR9I CCNGG 3 cut(s) 735, 981, 1017
MunI CAATTG 2 cut(s) 95, 1253
Mva1269I GAATGC 2 cut(s) 206, 1472
MvaI CCWGG 3 cut(s) 735, 981, 1017
MwoI GCNNNNNNNGC 3 cut(s) 377, 1478, 1487
NdeII GATC 8 cut(s) 127, 452, 565, 593, 709, 1064, 1139, 1425
NlaIII CATG 6 cut(s) 200, 347, 989, 1109, 1222, 1273
NlaIV GGNNCC 3 cut(s) 697, 739, 1415
NmuCI GTSAC 3 cut(s) 764, 1232, 1456
NspV TTCGAA 1 cut(s) 1476
PaeR7I CTCGAG 3 cut(s) 942, 1286, 1622
PceI AGGCCT 2 cut(s) 12, 733
PciSI GCTCTTC 2 cut(s) 35, 753
PctI GAATGC 2 cut(s) 206, 1472
PdmI GAANNNNTTC 2 cut(s) 1005, 1683
PfeI GAWTC 7 cut(s) 64, 404, 1025, 1259, 1388, 1511, 1532
PflFI GACNNNGTC 2 cut(s) 981, 1709
PflMI CCANNNNNTGG 1 cut(s) 986
Ple19I CGATCG 1 cut(s) 1142
PleI GAGTC 1 cut(s) 1162
PpsI GAGTC 1 cut(s) 1162
PshAI GACNNNNGTC 1 cut(s) 1352
Psp124BI GAGCTC 1 cut(s) 1623
Psp6I CCWGG 3 cut(s) 733, 979, 1015
PspGI CCWGG 3 cut(s) 733, 979, 1015
PspN4I GGNNCC 3 cut(s) 697, 739, 1415
PspXI VCTCGAGB 1 cut(s) 1622
PsuI RGATCY 2 cut(s) 452, 593
PsyI GACNNNGTC 2 cut(s) 981, 1709
PvuI CGATCG 1 cut(s) 1142
PvuII CAGCTG 1 cut(s) 1339
RsaI GTAC 5 cut(s) 162, 299, 774, 1036, 1099
RsaNI GTAC 5 cut(s) 161, 298, 773, 1035, 1098
RseI CAYNNNNRTG 1 cut(s) 504
SacI GAGCTC 1 cut(s) 1623
SapI GCTCTTC 2 cut(s) 35, 753
SaqAI TTAA 7 cut(s) 285, 786, 798, 1146, 1332, 1377, 1629
Sau3AI GATC 8 cut(s) 127, 452, 565, 593, 709, 1064, 1139, 1425
SchI GAGTC 1 cut(s) 1163
ScrFI CCNGG 3 cut(s) 735, 981, 1017
SduI GDGCHC 2 cut(s) 1609, 1623
SexAI ACCWGGT 1 cut(s) 979
SfaNI GCATC 7 cut(s) 85, 338, 496, 649, 844, 943, 1474
Sfr274I CTCGAG 3 cut(s) 942, 1286, 1622
SfuI TTCGAA 1 cut(s) 1476
SlaI CTCGAG 3 cut(s) 942, 1286, 1622
SmiMI CAYNNNNRTG 1 cut(s) 504
SmlI CTYRAG 5 cut(s) 217, 942, 1286, 1376, 1622
SmoI CTYRAG 5 cut(s) 217, 942, 1286, 1376, 1622
SseBI AGGCCT 2 cut(s) 12, 733
SsiI CCGC 7 cut(s) 44, 73, 361, 372, 699, 723, 1242
SspI AATATT 1 cut(s) 1006
SspMI CTAG 1 cut(s) 969
SstI GAGCTC 1 cut(s) 1623
StuI AGGCCT 2 cut(s) 12, 733
StyD4I CCNGG 3 cut(s) 733, 979, 1015
StyI CCWWGG 2 cut(s) 525, 1671
TaaI ACNGT 3 cut(s) 1039, 1181, 1502
TaiI ACGT 1 cut(s) 183
TaqI TCGA 7 cut(s) 637, 943, 1287, 1468, 1476, 1623, 1677
TaqII GACCGA 1 cut(s) 498
TatI WGTACW 1 cut(s) 297
TauI GCSGC 4 cut(s) 46, 76, 374, 1244
TfiI GAWTC 7 cut(s) 64, 404, 1025, 1259, 1388, 1511, 1532
Tru1I TTAA 7 cut(s) 285, 786, 798, 1146, 1332, 1377, 1629
Tru9I TTAA 7 cut(s) 285, 786, 798, 1146, 1332, 1377, 1629
TscAI CASTG 5 cut(s) 139, 211, 1165, 1560, 1651
TseFI GTSAC 3 cut(s) 764, 1232, 1456
TseI GCWGC 7 cut(s) 25, 276, 374, 377, 839, 1336, 1481
Tsp45I GTSAC 3 cut(s) 764, 1232, 1456
TspDTI ATGAA 5 cut(s) 213, 795, 998, 1014, 1143
TspGWI ACGGA 4 cut(s) 501, 510, 870, 1627
TspRI CASTG 5 cut(s) 139, 211, 1165, 1560, 1651
Tth111I GACNNNGTC 2 cut(s) 981, 1709
Van91I CCANNNNNTGG 1 cut(s) 986
Vha464I CTTAAG 1 cut(s) 1376
XapI RAATTY 4 cut(s) 100, 353, 909, 1192
XcmI CCANNNNNNNNNTGG 1 cut(s) 1165
XhoI CTCGAG 3 cut(s) 942, 1286, 1622
XmnI GAANNNNTTC 2 cut(s) 1005, 1683
XspI CTAG 1 cut(s) 969
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.