pycom10g12270

aminoacylase activity

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
pyrus_communis
Chr10
OG0004417
Physical Location & Seq
Reverse (-)
15809181 .. 15812689
3509 bp
Loading structure...
UTR
Exon/CDS
Intron
N/A

Sequence Viewer

Length: 1329 bp
ATGGAGCTTCGTCTTCGTCTCTGCATAATCTCTCTGCTACTGCTCTCATCAAAATCAACGGCCATACAATCCGACCACCCGATAATCTCACGGTTTCAACGATACCTCCAAATCAACACAGCCCACCCTAATCCCCAGTACCGCGAAGCAGCCGATTTCATTCTCTCCGAGGCCGAGTCCCTCTCCCTCCAGTCCCAGACTCTCGAGTTCGTCCCCGGCAAACCGCTCGTCCTCCTCAAATGGCCAGGCTCCGACCCTTCTCTCCCCTCCGTTCTCCTCAACTCCCACACCGACGTCGTCCCGGCCGAGCACGACAAGTGGGTCCACCCACCTTTCTCGGCCAACTTGGATCCGAACGGCGACGTTTACGCCAGGGGATCCCAGGACATGAAGTGCGTTGGGATTCAGTACCTGGAGGCCATTCGGCGGCTCAAGGCTTCTGGGTTTAAGCCCAAAAGGTCTGTTTACTTGTCGTTTGTGCCTGATGAGGAGATTGGGGGGCACGACGGCGCTGAGAAGCTGGCTGAATCTGATGTTTTCAAGGGATTGAACGTAGGAATTGTGCTTGATGAAGGGTTGGCCTCGCCAACTGAGAATTACAGGGCGTTCTATGCAGAGAGGTGTCCCATGTGGCTGGTGATCAAGGCTACTGGGGCTCCTGGTCATGGGGCTAAGCTCTATGACAACACTGCCATGGAGAATCTCTTGAAAAGCATTGAGAGTGTGAGGCGGTTCCGAGCTTCACAGTTTGATTTGGTGAAGGCAGGTTTGAAGGCTGAAGGCGAGGTCGTCTCTGTTAATATGGTGTTTTTGAAAGCTGGCACTCCAAGTCCAACAGGTTTTGTCATGAATTTGCAGCCATCTGAAGCAGAAGCAGGCTTTGATATTAGGGTCCCACCAACTGCTGATCAGGAGTCTTTAGAGAAACGGATAGCTGAAGAATGGGCCCCTGCTTCACGAAACATGACATTCAGGTTCAAGCAGAAGGTTTCTGTGCTTGATAAGGCGGGGAGGCCAATCCTTACAGCAACTGATAGTTCAAACCCCTGGTGGGCTCTTCTTGAAGATGCTGTCAAAAAAGCAAATGGGAAACTTGGTAAGCCAGAGATTTTTCCTGCTTCAACAGATGCTCGCTACTTCCGGAATCTGGGCTTGCCAGCAATTGGATTCTCTCCGATGGCTAACACTCCTATTCTTCTTCATGACCATAACGAGTTTCTAAACAAAGACGAATACTTGAAAGGAATTGATGTTTACGAATCTATTATCAAGGCCTATGCATCTTACGTTGATGGTGGGAGAAGCGCGGTTTCCAGAGATGAGTTGTAA

Protein Analysis

443

Amino Acids

48.88

Weight (kDa)

6.05

Isoelectric Point (pI)

39.75

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
Peptidase_M20 PF01546 92 - 424 5e-36 Peptidase family M20/M25/M40
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AasI GACNNNNNNGTC 1 cut(s) 320
AatII GACGTC 1 cut(s) 297
Acc36I ACCTGC 1 cut(s) 755
AccB7I CCANNNNNTGG 1 cut(s) 1163
AccBSI CCGCTC 1 cut(s) 226
AccII CGCG 2 cut(s) 144, 1307
AccIII TCCGGA 1 cut(s) 1140
AciI CCGC 6 cut(s) 142, 224, 427, 730, 1007, 1307
AclWI GGATC 4 cut(s) 344, 357, 372, 385
AcoI YGGCCR 4 cut(s) 60, 242, 303, 339
AcsI RAATTY 1 cut(s) 850
AcuI CTGAAG 3 cut(s) 798, 885, 957
AcyI GRCGYC 1 cut(s) 294
AfaI GTAC 2 cut(s) 140, 410
AfiI CCNNNNNNNGG 5 cut(s) 426, 665, 1051, 1147, 1163
AjnI CCWGG 6 cut(s) 244, 371, 381, 411, 658, 1046
AluBI AGCT 6 cut(s) 7, 520, 676, 740, 818, 935
AluI AGCT 6 cut(s) 7, 520, 676, 740, 818, 935
Alw21I GWGCWC 1 cut(s) 312
Alw26I GTCTC 2 cut(s) 23, 796
AlwI GGATC 4 cut(s) 344, 357, 372, 385
AlwNI CAGNNNCTG 2 cut(s) 412, 1031
Ama87I CYCGRG 1 cut(s) 203
Aor13HI TCCGGA 1 cut(s) 1140
ApaI GGGCCC 1 cut(s) 949
ApeKI GCWGC 2 cut(s) 149, 856
ApoI RAATTY 1 cut(s) 850
ArsI GACNNNNNNTTYG 2 cut(s) 213, 245
AspLEI GCGC 2 cut(s) 512, 1307
AspS9I GGNCC 4 cut(s) 322, 892, 945, 946
AsuC2I CCSGG 2 cut(s) 216, 302
AsuHPI GGTGA 2 cut(s) 649, 769
AvaI CYCGRG 1 cut(s) 203
AvaII GGWCC 2 cut(s) 322, 892
BaeGI GKGCMC 2 cut(s) 504, 949
BalI TGGCCA 1 cut(s) 244
BamHI GGATCC 2 cut(s) 349, 377
BanII GRGCYC 3 cut(s) 658, 949, 1057
BbsI GAAGAC 1 cut(s) 5
Bbv12I GWGCWC 1 cut(s) 312
BbvI GCAGC 2 cut(s) 161, 868
BccI CCATC 3 cut(s) 868, 1171, 1286
BceAI ACGGC 3 cut(s) 75, 373, 523
BcgI CGANNNNNNTGC 2 cut(s) 208, 242
BciT130I CCWGG 6 cut(s) 246, 373, 383, 413, 660, 1048
BclI TGATCA 2 cut(s) 639, 907
BcnI CCSGG 2 cut(s) 216, 302
BcoDI GTCTC 2 cut(s) 23, 796
BfoI RGCGCY 1 cut(s) 513
BfuAI ACCTGC 1 cut(s) 755
BisI GCNGC 3 cut(s) 150, 428, 857
BlpI GCTNAGC 1 cut(s) 672
BlsI GCNGC 3 cut(s) 151, 429, 858
Bme1390I CCNGG 8 cut(s) 216, 246, 302, 373, 383, 413, 660, 1048
Bme18I GGWCC 2 cut(s) 322, 892
BmeT110I CYCGRG 1 cut(s) 203
BmgT120I GGNCC 4 cut(s) 322, 892, 945, 946
BmrFI CCNGG 8 cut(s) 216, 246, 302, 373, 383, 413, 660, 1048
BmrI ACTGGG 2 cut(s) 130, 660
BmsI GCATC 3 cut(s) 1057, 1117, 1289
BmuI ACTGGG 2 cut(s) 130, 660
BpiI GAAGAC 1 cut(s) 5
BplI GAGNNNNNCTC 4 cut(s) 167, 199, 776, 808
BpmI CTGGAG 2 cut(s) 173, 434
Bpu1102I GCTNAGC 1 cut(s) 672
BpuEI CTTGAG 1 cut(s) 416
BpuMI CCSGG 2 cut(s) 216, 302
BsaHI GRCGYC 1 cut(s) 294
BsaJI CCNNGG 6 cut(s) 168, 214, 372, 381, 693, 1046
BsaWI WCCGGW 1 cut(s) 1140
BsaXI ACNNNNNCTCC 4 cut(s) 90, 120, 640, 670
Bsc4I CCNNNNNNNGG 5 cut(s) 426, 665, 1051, 1147, 1163
Bse1I ACTGG 3 cut(s) 136, 190, 655
BseAI TCCGGA 1 cut(s) 1140
BseBI CCWGG 6 cut(s) 246, 373, 383, 413, 660, 1048
BseDI CCNNGG 6 cut(s) 168, 214, 372, 381, 693, 1046
BseLI CCNNNNNNNGG 5 cut(s) 426, 665, 1051, 1147, 1163
BseMII CTCAG 2 cut(s) 504, 582
BseNI ACTGG 3 cut(s) 136, 190, 655
BseRI GAGGAG 3 cut(s) 224, 266, 503
BseSI GKGCMC 2 cut(s) 504, 949
BseX3I CGGCCG 1 cut(s) 303
BseXI GCAGC 2 cut(s) 161, 868
Bsh1236I CGCG 2 cut(s) 144, 1307
Bsh1285I CGRYCG 1 cut(s) 306
BsiEI CGRYCG 1 cut(s) 306
BsiHKAI GWGCWC 1 cut(s) 312
BsiHKCI CYCGRG 1 cut(s) 203
BsiSI CCGG 3 cut(s) 216, 302, 1141
BslFI GGGAC 6 cut(s) 163, 178, 197, 284, 609, 878
BslI CCNNNNNNNGG 5 cut(s) 426, 665, 1051, 1147, 1163
BsmAI GTCTC 2 cut(s) 23, 796
BsmBI CGTCTC 2 cut(s) 23, 796
BsmFI GGGAC 6 cut(s) 163, 178, 197, 284, 609, 878
BsoBI CYCGRG 1 cut(s) 203
Bsp120I GGGCCC 1 cut(s) 945
Bsp1286I GDGCHC 5 cut(s) 312, 504, 658, 949, 1057
Bsp13I TCCGGA 1 cut(s) 1140
Bsp143I GATC 4 cut(s) 349, 377, 639, 907
Bsp1720I GCTNAGC 1 cut(s) 672
Bsp19I CCATGG 1 cut(s) 693
BspACI CCGC 6 cut(s) 142, 224, 427, 730, 1007, 1307
BspCNI CTCAG 2 cut(s) 505, 583
BspEI TCCGGA 1 cut(s) 1140
BspFNI CGCG 2 cut(s) 144, 1307
BspHI TCATGA 2 cut(s) 846, 1201
BspMI ACCTGC 1 cut(s) 755
BspPI GGATC 4 cut(s) 344, 357, 372, 385
BspQI GCTCTTC 1 cut(s) 1062
BsrBI CCGCTC 1 cut(s) 226
BsrI ACTGG 3 cut(s) 136, 190, 655
BssECI CCNNGG 6 cut(s) 168, 214, 372, 381, 693, 1046
BssMI GATC 4 cut(s) 349, 377, 639, 907
BssNI GRCGYC 1 cut(s) 294
BssT1I CCWWGG 1 cut(s) 693
Bst2UI CCWGG 6 cut(s) 246, 373, 383, 413, 660, 1048
Bst4CI ACNGT 2 cut(s) 93, 747
Bst6I CTCTTC 1 cut(s) 1062
BstACI GRCGYC 1 cut(s) 294
BstC8I GCNNGC 6 cut(s) 522, 820, 877, 1132, 1154, 1158
BstDEI CTNAG 3 cut(s) 513, 591, 672
BstDSI CCRYGG 1 cut(s) 693
BstFNI CGCG 2 cut(s) 144, 1307
BstH2I RGCGCY 1 cut(s) 513
BstHHI GCGC 2 cut(s) 512, 1307
BstKTI GATC 4 cut(s) 352, 380, 642, 910
BstMAI GTCTC 2 cut(s) 23, 796
BstMBI GATC 4 cut(s) 349, 377, 639, 907
BstMCI CGRYCG 1 cut(s) 306
BstMWI GCNNNNNNNGC 2 cut(s) 611, 653
BstNI CCWGG 6 cut(s) 246, 373, 383, 413, 660, 1048
BstSCI CCNGG 8 cut(s) 214, 244, 300, 371, 381, 411, 658, 1046
BstSLI GKGCMC 2 cut(s) 504, 949
BstUI CGCG 2 cut(s) 144, 1307
BstV1I GCAGC 2 cut(s) 161, 868
BstV2I GAAGAC 1 cut(s) 5
BstX2I RGATCY 2 cut(s) 349, 377
BstXI CCANNNNNNTGG 1 cut(s) 634
BstYI RGATCY 2 cut(s) 349, 377
BstZI CGGCCG 1 cut(s) 303
BtgI CCRYGG 1 cut(s) 693
BtsI GCAGTG 1 cut(s) 687
BtsIMutI CAGTG 1 cut(s) 687
BveI ACCTGC 1 cut(s) 755
Cac8I GCNNGC 6 cut(s) 522, 820, 877, 1132, 1154, 1158
CaiI CAGNNNCTG 2 cut(s) 412, 1031
CciI TCATGA 2 cut(s) 846, 1201
CfoI GCGC 2 cut(s) 512, 1307
Cfr13I GGNCC 4 cut(s) 322, 892, 945, 946
Csp6I GTAC 2 cut(s) 139, 409
CviAII CATG 7 cut(s) 388, 628, 665, 694, 847, 964, 1202
CviQI GTAC 2 cut(s) 139, 409
DdeI CTNAG 3 cut(s) 513, 591, 672
DpnI GATC 4 cut(s) 351, 379, 641, 909
DpnII GATC 4 cut(s) 349, 377, 639, 907
DrdI GACNNNNNNGTC 1 cut(s) 320
DseDI GACNNNNNNGTC 1 cut(s) 320
EaeI YGGCCR 4 cut(s) 60, 242, 303, 339
EagI CGGCCG 1 cut(s) 303
Eam1104I CTCTTC 1 cut(s) 1062
EarI CTCTTC 1 cut(s) 1062
EclXI CGGCCG 1 cut(s) 303
Eco130I CCWWGG 1 cut(s) 693
Eco147I AGGCCT 1 cut(s) 1274
Eco24I GRGCYC 3 cut(s) 658, 949, 1057
Eco47I GGWCC 2 cut(s) 322, 892
Eco52I CGGCCG 1 cut(s) 303
Eco57I CTGAAG 3 cut(s) 798, 885, 957
Eco88I CYCGRG 1 cut(s) 203
EcoO109I RGGNCCY 2 cut(s) 892, 946
EcoRII CCWGG 6 cut(s) 244, 371, 381, 411, 658, 1046
EcoT14I CCWWGG 1 cut(s) 693
EcoT22I ATGCAT 1 cut(s) 1282
EcoT38I GRGCYC 3 cut(s) 658, 949, 1057
ErhI CCWWGG 1 cut(s) 693
Esp3I CGTCTC 2 cut(s) 23, 796
FaeI CATG 7 cut(s) 391, 631, 668, 697, 850, 967, 1205
FaqI GGGAC 6 cut(s) 163, 178, 197, 284, 609, 878
FatI CATG 7 cut(s) 387, 627, 664, 693, 846, 963, 1201
FauI CCCGC 1 cut(s) 1000
FbaI TGATCA 2 cut(s) 639, 907
Fnu4HI GCNGC 3 cut(s) 150, 428, 857
FriOI GRGCYC 3 cut(s) 658, 949, 1057
Fsp4HI GCNGC 3 cut(s) 150, 428, 857
GlaI GCGC 2 cut(s) 511, 1306
GluI GCNGC 3 cut(s) 150, 428, 857
GsuI CTGGAG 2 cut(s) 173, 434
HaeII RGCGCY 1 cut(s) 513
HapII CCGG 3 cut(s) 216, 302, 1141
HhaI GCGC 2 cut(s) 512, 1307
Hin1I GRCGYC 1 cut(s) 294
Hin1II CATG 7 cut(s) 391, 631, 668, 697, 850, 967, 1205
Hin6I GCGC 2 cut(s) 510, 1305
HinP1I GCGC 2 cut(s) 510, 1305
HinfI GANTC 9 cut(s) 176, 199, 403, 527, 700, 914, 1144, 1167, 1259
HpaII CCGG 3 cut(s) 216, 302, 1141
HphI GGTGA 2 cut(s) 649, 769
Hpy166II GTNNAC 4 cut(s) 325, 367, 466, 1255
Hpy188I TCNGA 8 cut(s) 73, 169, 253, 354, 532, 737, 865, 1176
Hpy188III TCNNGA 9 cut(s) 203, 706, 847, 911, 957, 1061, 1141, 1202, 1314
Hpy8I GTNNAC 4 cut(s) 325, 367, 466, 1255
Hpy99I CGWCG 4 cut(s) 296, 299, 365, 509
HpyAV CCTTC 6 cut(s) 267, 566, 754, 766, 773, 979
HpyCH4III ACNGT 2 cut(s) 93, 747
HpyCH4IV ACGT 4 cut(s) 294, 363, 552, 1287
HpyCH4V TGCA 4 cut(s) 24, 614, 856, 1280
HpyF10VI GCNNNNNNNGC 2 cut(s) 611, 653
HpyF3I CTNAG 3 cut(s) 513, 591, 672
HpySE526I ACGT 4 cut(s) 294, 363, 552, 1287
Hsp92I GRCGYC 1 cut(s) 294
Hsp92II CATG 7 cut(s) 391, 631, 668, 697, 850, 967, 1205
HspAI GCGC 2 cut(s) 510, 1305
KflI GGGWCCC 1 cut(s) 892
Kpn2I TCCGGA 1 cut(s) 1140
Ksp22I TGATCA 2 cut(s) 639, 907
Kzo9I GATC 4 cut(s) 349, 377, 639, 907
LguI GCTCTTC 1 cut(s) 1062
LmnI GCTCC 3 cut(s) 4, 254, 661
Lsp1109I GCAGC 2 cut(s) 161, 868
LweI GCATC 3 cut(s) 1057, 1117, 1289
MaeII ACGT 4 cut(s) 294, 363, 552, 1287
MalI GATC 4 cut(s) 351, 379, 641, 909
MbiI CCGCTC 1 cut(s) 226
MboI GATC 4 cut(s) 349, 377, 639, 907
MboII GAAGA 6 cut(s) 5, 950, 1049, 1076, 1187, 1190
MfeI CAATTG 1 cut(s) 1161
MflI RGATCY 2 cut(s) 349, 377
MhlI GDGCHC 5 cut(s) 312, 504, 658, 949, 1057
MlsI TGGCCA 1 cut(s) 244
MluCI AATT 5 cut(s) 558, 595, 850, 1161, 1245
MluNI TGGCCA 1 cut(s) 244
MlyI GAGTC 3 cut(s) 185, 193, 923
MmeI TCCRAC 3 cut(s) 96, 276, 857
Mox20I TGGCCA 1 cut(s) 244
Mph1103I ATGCAT 1 cut(s) 1282
MroI TCCGGA 1 cut(s) 1140
MscI TGGCCA 1 cut(s) 244
MseI TTAA 2 cut(s) 447, 798
MslI CAYNNNNRTG 1 cut(s) 692
Msp20I TGGCCA 1 cut(s) 244
MspI CCGG 3 cut(s) 216, 302, 1141
MspR9I CCNGG 8 cut(s) 216, 246, 302, 373, 383, 413, 660, 1048
MunI CAATTG 1 cut(s) 1161
MvaI CCWGG 6 cut(s) 246, 373, 383, 413, 660, 1048
MvnI CGCG 2 cut(s) 144, 1307
MwoI GCNNNNNNNGC 2 cut(s) 611, 653
NciI CCSGG 2 cut(s) 216, 302
NcoI CCATGG 1 cut(s) 693
NdeII GATC 4 cut(s) 349, 377, 639, 907
NlaIII CATG 7 cut(s) 391, 631, 668, 697, 850, 967, 1205
NmeAIII GCCGAG 3 cut(s) 199, 317, 331
NsiI ATGCAT 1 cut(s) 1282
PaeR7I CTCGAG 1 cut(s) 203
PagI TCATGA 2 cut(s) 846, 1201
PceI AGGCCT 1 cut(s) 1274
PciSI GCTCTTC 1 cut(s) 1062
PcsI WCGNNNNNNNCGW 2 cut(s) 97, 303
PfeI GAWTC 6 cut(s) 403, 527, 700, 1144, 1167, 1259
PflFI GACNNNGTC 1 cut(s) 296
PflMI CCANNNNNTGG 1 cut(s) 1163
PkrI GCNGC 3 cut(s) 151, 429, 858
PleI GAGTC 3 cut(s) 184, 193, 922
PpsI GAGTC 3 cut(s) 184, 193, 922
PpuMI RGGWCCY 1 cut(s) 892
Psp5II RGGWCCY 1 cut(s) 892
Psp6I CCWGG 6 cut(s) 244, 371, 381, 411, 658, 1046
PspGI CCWGG 6 cut(s) 244, 371, 381, 411, 658, 1046
PspOMI GGGCCC 1 cut(s) 945
PspPI GGNCC 4 cut(s) 322, 892, 945, 946
PspPPI RGGWCCY 1 cut(s) 892
PstNI CAGNNNCTG 2 cut(s) 412, 1031
PsuI RGATCY 2 cut(s) 349, 377
PsyI GACNNNGTC 1 cut(s) 296
RsaI GTAC 2 cut(s) 140, 410
RsaNI GTAC 2 cut(s) 139, 409
RseI CAYNNNNRTG 1 cut(s) 692
SapI GCTCTTC 1 cut(s) 1062
SaqAI TTAA 2 cut(s) 447, 798
SatI GCNGC 3 cut(s) 150, 428, 857
Sau3AI GATC 4 cut(s) 349, 377, 639, 907
Sau96I GGNCC 4 cut(s) 322, 892, 945, 946
SchI GAGTC 3 cut(s) 185, 193, 923
ScrFI CCNGG 8 cut(s) 216, 246, 302, 373, 383, 413, 660, 1048
SduI GDGCHC 5 cut(s) 312, 504, 658, 949, 1057
SfaNI GCATC 3 cut(s) 1057, 1117, 1289
Sfr274I CTCGAG 1 cut(s) 203
SinI GGWCC 2 cut(s) 322, 892
SlaI CTCGAG 1 cut(s) 203
SmiMI CAYNNNNRTG 1 cut(s) 692
SmlI CTYRAG 2 cut(s) 203, 431
SmoI CTYRAG 2 cut(s) 203, 431
Sse9I AATT 5 cut(s) 558, 595, 850, 1161, 1245
SseBI AGGCCT 1 cut(s) 1274
SsiI CCGC 6 cut(s) 142, 224, 427, 730, 1007, 1307
StuI AGGCCT 1 cut(s) 1274
StyD4I CCNGG 8 cut(s) 214, 244, 300, 371, 381, 411, 658, 1046
StyI CCWWGG 1 cut(s) 693
TaaI ACNGT 2 cut(s) 93, 747
TaiI ACGT 4 cut(s) 297, 366, 555, 1290
TaqI TCGA 1 cut(s) 204
TasI AATT 5 cut(s) 558, 595, 850, 1161, 1245
TauI GCSGC 1 cut(s) 430
TfiI GAWTC 6 cut(s) 403, 527, 700, 1144, 1167, 1259
Tru1I TTAA 2 cut(s) 447, 798
Tru9I TTAA 2 cut(s) 447, 798
TscAI CASTG 1 cut(s) 694
TseI GCWGC 2 cut(s) 149, 856
TspDTI ATGAA 5 cut(s) 148, 404, 585, 863, 1190
TspGWI ACGGA 2 cut(s) 259, 943
TspRI CASTG 1 cut(s) 694
Tth111I GACNNNGTC 1 cut(s) 296
Van91I CCANNNNNTGG 1 cut(s) 1163
VpaK11BI GGWCC 2 cut(s) 322, 892
XapI RAATTY 1 cut(s) 850
XhoI CTCGAG 1 cut(s) 203
ZraI GACGTC 1 cut(s) 295
Zsp2I ATGCAT 1 cut(s) 1282
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.