pycom10g12320

Brefeldin A-inhibited guanine nucleotide-exchange protein

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
pyrus_communis
Chr10
OG0002933
Physical Location & Seq
Forward (+)
15847090 .. 15848037
948 bp
Loading structure...
UTR
Exon/CDS
Intron
N/A

Sequence Viewer

Length: 948 bp
ATGTCCGCCTCCCAAACCCTAGGTGGCCCCTCCCGCGCCGGCCGCCTTCTCGGCCCCTCTCTCGACAAAATCATCAAGAACGCCGCCTGGCGCAAGCACTCCCATTTGGTCTCCGCCTGCAAATCCGCCCTCGACAAGCTCGACTCCGTCACCGACTCCTCCATCATCGATCCCAAGTCCCCCGTCTCCGGCATCTCCCTCGCCGACGCCGAGTTCGCCCTCCGCCCCGTCCTCCTCGCCCTAGAATCGGCTTACCCCAAGGTCGTCGAGCCCGCCATCGACTGCGTCTTCAAGCTTTTCTCCCTCGGTATCTTCCGCGGTGAGATCGACACTTCCGATCCCAAATTTGTGCTTTTCAAGCTCGTCGAGTCGGTGTGTAAGTGCGCGGCGATCAGCGAGGAGCCTATCGAGCTCGGCGTGCTTCGGGTATTGCTCGCCGCCGTGAGATCGCCGCGGGTTTTGATACGCGGTGATTGTTTGGTGAATATTGTTAGGACTTGTTACAATGTGTACCTTCGTGGAGTGAATGGGACCAATCAGATCTGCGCCAAGTCGGTTTTGGCTCAGATTATGGAGATTGTTTTCACAAGAGTAGAGGAGGATTCGGTGAATGTCAGCATTTCTAGGGTTTCGGTGAACGAGTTGTTGGAATTCACTGATAAAAATTTGAACGAAGGGAGCTCCATTTTGTTATGCCAGAATTTCGTCAATGAGGTTATGGATGCAAGCTACGGCGGTCCGGATGGTATCAAAACGGTTGCCCCCCCAGGTCCCAAATTACAAAACGGCAATGCCAGTGGTGAGTCTAATAATGATGGAGCTGAATCCGGCGACGGTGCTAGTAAGATTAGGGATGATGGGTATCTTTTGTTTAAGAATTTGTGTAAGTTGTCCATGAAATACTCATCACAGGAGCACTCTGATCGATCAAATTCTCTTGAGGGGTAA
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
GO:0000139 GO:0003674 GO:0005085 GO:0005086 GO:0005488 GO:0005515 GO:0005575 GO:0005622 GO:0005623 GO:0005634 GO:0005654 GO:0005730 GO:0005737 GO:0005794 GO:0005802 GO:0005829 GO:0006810 GO:0006887 GO:0006996 GO:0007030 GO:0008064 GO:0008092 GO:0008150 GO:0009507 GO:0009536 GO:0009889 GO:0009891 GO:0009893 GO:0009987 GO:0010256 GO:0010556 GO:0010557 GO:0010559 GO:0010560 GO:0010604 GO:0010639 GO:0012505 GO:0016020 GO:0016043 GO:0016192 GO:0016363 GO:0017022 GO:0019222 GO:0019899 GO:0030532 GO:0030832 GO:0030833 GO:0030837 GO:0031090 GO:0031323 GO:0031325 GO:0031326 GO:0031328 GO:0031333 GO:0031399 GO:0031401 GO:0031974 GO:0031981 GO:0031984 GO:0032268 GO:0032270 GO:0032271 GO:0032272 GO:0032535 GO:0032588 GO:0032878 GO:0032940 GO:0032956 GO:0032970 GO:0032991 GO:0033043 GO:0034237 GO:0034260 GO:0034399 GO:0043086 GO:0043087 GO:0043226 GO:0043227 GO:0043228 GO:0043229 GO:0043231 GO:0043232 GO:0043233 GO:0043254 GO:0044087 GO:0044092 GO:0044422 GO:0044424 GO:0044428 GO:0044431 GO:0044444 GO:0044446 GO:0044464 GO:0046903 GO:0048471 GO:0048518 GO:0048519 GO:0048522 GO:0048523 GO:0048583 GO:0048584 GO:0050789 GO:0050790 GO:0050794 GO:0051018 GO:0051020 GO:0051128 GO:0051129 GO:0051171 GO:0051173 GO:0051179 GO:0051234 GO:0051246 GO:0051247 GO:0051336 GO:0051346 GO:0051493 GO:0051494 GO:0060049 GO:0060050 GO:0060255 GO:0061041 GO:0065007 GO:0065008 GO:0065009 GO:0070013 GO:0071840 GO:0080090 GO:0080134 GO:0090066 GO:0090283 GO:0090284 GO:0090303 GO:0098588 GO:0098772 GO:0098791 GO:0110053 GO:0120114 GO:1902903 GO:1902904 GO:1903018 GO:1903020 GO:1903034 GO:1903036 GO:1990904 GO:2000112 GO:2000114
KEGG Pathways
Metabolic & Signaling
ko04144 map04144
Pfam Domains
Protein Families
DCB DUF1981 Mon2_C Sec7 Sec7_N

Protein Analysis

316

Amino Acids

33.87

Weight (kDa)

5.57

Isoelectric Point (pI)

32.17

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
DCB PF16213 19 - 201 3.4e-25 Mon2/Sec7/BIG1-like, dimerisation and cyclophilin-binding domain
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AccII CGCG 5 cut(s) 36, 318, 386, 454, 468
AccIII TCCGGA 1 cut(s) 739
AclWI GGATC 2 cut(s) 164, 332
AcoI YGGCCR 1 cut(s) 40
AcsI RAATTY 6 cut(s) 344, 650, 664, 700, 877, 931
AcyI GRCGYC 1 cut(s) 207
AfaI GTAC 1 cut(s) 512
AfiI CCNNNNNNNGG 2 cut(s) 188, 247
AgsI TTSAA 3 cut(s) 292, 358, 670
AjnI CCWGG 2 cut(s) 86, 766
AjuI GAANNNNNNNTTGG 2 cut(s) 629, 661
AluBI AGCT 7 cut(s) 139, 295, 361, 412, 681, 729, 821
AluI AGCT 7 cut(s) 139, 295, 361, 412, 681, 729, 821
Alw21I GWGCWC 3 cut(s) 414, 683, 918
Alw26I GTCTC 2 cut(s) 115, 190
AlwI GGATC 2 cut(s) 164, 332
Aor13HI TCCGGA 1 cut(s) 739
AoxI GGCC 3 cut(s) 25, 40, 52
ApoI RAATTY 6 cut(s) 344, 650, 664, 700, 877, 931
ArsI GACNNNNNNTTYG 2 cut(s) 197, 229
AspA2I CCTAGG 1 cut(s) 19
AspLEI GCGC 4 cut(s) 38, 93, 386, 548
AspS9I GGNCC 5 cut(s) 26, 53, 531, 737, 770
AsuHPI GGTGA 7 cut(s) 142, 332, 482, 493, 619, 646, 812
AvaII GGWCC 3 cut(s) 531, 737, 770
AvrII CCTAGG 1 cut(s) 19
BanII GRGCYC 3 cut(s) 273, 414, 683
BbsI GAAGAC 1 cut(s) 280
Bbv12I GWGCWC 3 cut(s) 414, 683, 918
BccI CCATC 5 cut(s) 170, 284, 737, 809, 851
BceAI ACGGC 3 cut(s) 425, 748, 802
BcgI CGANNNNNNTGC 4 cut(s) 181, 215, 905, 939
BciT130I CCWGG 2 cut(s) 88, 768
BcoDI GTCTC 2 cut(s) 115, 190
BfaI CTAG 4 cut(s) 20, 242, 624, 840
BglI GCCNNNNNGGC 1 cut(s) 51
BglII AGATCT 1 cut(s) 540
BisI GCNGC 5 cut(s) 43, 84, 387, 438, 452
BlnI CCTAGG 1 cut(s) 19
BlsI GCNGC 5 cut(s) 44, 85, 388, 439, 453
Bme1390I CCNGG 2 cut(s) 88, 768
Bme18I GGWCC 3 cut(s) 531, 737, 770
BmgT120I GGNCC 5 cut(s) 26, 53, 531, 737, 770
BmiI GGNNCC 5 cut(s) 28, 55, 402, 532, 772
BmrFI CCNGG 2 cut(s) 88, 768
BmsI GCATC 2 cut(s) 201, 712
BpiI GAAGAC 1 cut(s) 280
Bsa29I ATCGAT 2 cut(s) 168, 925
BsaBI GATNNNNATC 1 cut(s) 861
BsaHI GRCGYC 1 cut(s) 207
BsaI GGTCTC 1 cut(s) 115
BsaJI CCNNGG 6 cut(s) 19, 258, 304, 316, 452, 766
BsaWI WCCGGW 1 cut(s) 739
Bsc4I CCNNNNNNNGG 2 cut(s) 188, 247
Bse118I RCCGGY 1 cut(s) 38
Bse1I ACTGG 1 cut(s) 795
Bse3DI GCAATG 1 cut(s) 796
Bse8I GATNNNNATC 1 cut(s) 861
BseAI TCCGGA 1 cut(s) 739
BseBI CCWGG 2 cut(s) 88, 768
BseCI ATCGAT 2 cut(s) 168, 925
BseDI CCNNGG 6 cut(s) 19, 258, 304, 316, 452, 766
BseGI GGATG 3 cut(s) 727, 748, 859
BseJI GATNNNNATC 1 cut(s) 861
BseLI CCNNNNNNNGG 2 cut(s) 188, 247
BseMI GCAATG 1 cut(s) 796
BseMII CTCAG 1 cut(s) 578
BseNI ACTGG 1 cut(s) 795
BseRI GAGGAG 4 cut(s) 148, 224, 413, 611
BseX3I CGGCCG 1 cut(s) 40
Bsh1236I CGCG 5 cut(s) 36, 318, 386, 454, 468
Bsh1285I CGRYCG 1 cut(s) 43
BshFI GGCC 3 cut(s) 27, 42, 54
BshVI ATCGAT 2 cut(s) 168, 925
BsiEI CGRYCG 1 cut(s) 43
BsiHKAI GWGCWC 3 cut(s) 414, 683, 918
BsiSI CCGG 4 cut(s) 39, 189, 740, 828
BslFI GGGAC 3 cut(s) 163, 544, 756
BslI CCNNNNNNNGG 2 cut(s) 188, 247
BsmAI GTCTC 2 cut(s) 115, 190
BsmBI CGTCTC 1 cut(s) 190
BsmFI GGGAC 3 cut(s) 163, 544, 756
BsnI GGCC 3 cut(s) 27, 42, 54
Bso31I GGTCTC 1 cut(s) 115
Bsp1286I GDGCHC 4 cut(s) 273, 414, 683, 918
Bsp13I TCCGGA 1 cut(s) 739
Bsp143I GATC 8 cut(s) 169, 324, 337, 390, 446, 540, 922, 926
BspANI GGCC 3 cut(s) 27, 42, 54
BspCNI CTCAG 1 cut(s) 577
BspDI ATCGAT 2 cut(s) 168, 925
BspEI TCCGGA 1 cut(s) 739
BspFNI CGCG 5 cut(s) 36, 318, 386, 454, 468
BspLI GGNNCC 5 cut(s) 28, 55, 402, 532, 772
BspPI GGATC 2 cut(s) 164, 332
BspTNI GGTCTC 1 cut(s) 115
BsrDI GCAATG 1 cut(s) 796
BsrFI RCCGGY 1 cut(s) 38
BsrI ACTGG 1 cut(s) 795
BssAI RCCGGY 1 cut(s) 38
BssECI CCNNGG 6 cut(s) 19, 258, 304, 316, 452, 766
BssMI GATC 8 cut(s) 169, 324, 337, 390, 446, 540, 922, 926
BssNI GRCGYC 1 cut(s) 207
BssT1I CCWWGG 2 cut(s) 19, 258
Bst2UI CCWGG 2 cut(s) 88, 768
Bst4CI ACNGT 2 cut(s) 757, 836
BstACI GRCGYC 1 cut(s) 207
BstC8I GCNNGC 7 cut(s) 40, 95, 118, 273, 419, 435, 727
BstDEI CTNAG 1 cut(s) 564
BstDSI CCRYGG 2 cut(s) 316, 452
BstF5I GGATG 3 cut(s) 727, 748, 859
BstFNI CGCG 5 cut(s) 36, 318, 386, 454, 468
BstHHI GCGC 4 cut(s) 38, 93, 386, 548
BstKTI GATC 8 cut(s) 172, 327, 340, 393, 449, 543, 925, 929
BstMAI GTCTC 2 cut(s) 115, 190
BstMBI GATC 8 cut(s) 169, 324, 337, 390, 446, 540, 922, 926
BstMCI CGRYCG 1 cut(s) 43
BstMWI GCNNNNNNNGC 7 cut(s) 33, 42, 51, 215, 358, 409, 418
BstNI CCWGG 2 cut(s) 88, 768
BstSCI CCNGG 2 cut(s) 86, 766
BstUI CGCG 5 cut(s) 36, 318, 386, 454, 468
BstV2I GAAGAC 1 cut(s) 280
BstX2I RGATCY 1 cut(s) 540
BstYI RGATCY 1 cut(s) 540
BstZI CGGCCG 1 cut(s) 40
Bsu15I ATCGAT 2 cut(s) 168, 925
BsuRI GGCC 3 cut(s) 27, 42, 54
BsuTUI ATCGAT 2 cut(s) 168, 925
BtgI CCRYGG 2 cut(s) 316, 452
BtsCI GGATG 3 cut(s) 727, 748, 859
BtsIMutI CAGTG 2 cut(s) 654, 802
Cac8I GCNNGC 7 cut(s) 40, 95, 118, 273, 419, 435, 727
CfoI GCGC 4 cut(s) 38, 93, 386, 548
Cfr10I RCCGGY 1 cut(s) 38
Cfr13I GGNCC 5 cut(s) 26, 53, 531, 737, 770
Cfr42I CCGCGG 2 cut(s) 319, 455
ClaI ATCGAT 2 cut(s) 168, 925
CpoI CGGWCCG 1 cut(s) 737
CseI GACGC 2 cut(s) 215, 274
Csp6I GTAC 1 cut(s) 511
CspCI CAANNNNNGTGG 2 cut(s) 778, 813
CspI CGGWCCG 1 cut(s) 737
CviAII CATG 1 cut(s) 895
CviQI GTAC 1 cut(s) 511
DdeI CTNAG 1 cut(s) 564
DpnI GATC 8 cut(s) 171, 326, 339, 392, 448, 542, 924, 928
DpnII GATC 8 cut(s) 169, 324, 337, 390, 446, 540, 922, 926
EaeI YGGCCR 1 cut(s) 40
EagI CGGCCG 1 cut(s) 40
EciI GGCGGA 3 cut(s) 103, 115, 212
Ecl136II GAGCTC 2 cut(s) 412, 681
EclXI CGGCCG 1 cut(s) 40
Eco130I CCWWGG 2 cut(s) 19, 258
Eco24I GRGCYC 3 cut(s) 273, 414, 683
Eco31I GGTCTC 1 cut(s) 115
Eco47I GGWCC 3 cut(s) 531, 737, 770
Eco52I CGGCCG 1 cut(s) 40
Eco53kI GAGCTC 2 cut(s) 412, 681
EcoICRI GAGCTC 2 cut(s) 412, 681
EcoO109I RGGNCCY 1 cut(s) 770
EcoRI GAATTC 1 cut(s) 650
EcoRII CCWGG 2 cut(s) 86, 766
EcoT14I CCWWGG 2 cut(s) 19, 258
EcoT38I GRGCYC 3 cut(s) 273, 414, 683
ErhI CCWWGG 2 cut(s) 19, 258
Esp3I CGTCTC 1 cut(s) 190
FaeI CATG 1 cut(s) 898
FaiI YATR 4 cut(s) 572, 694, 719, 896
FaqI GGGAC 3 cut(s) 163, 544, 756
FatI CATG 1 cut(s) 894
FauI CCCGC 3 cut(s) 41, 280, 447
Fnu4HI GCNGC 5 cut(s) 43, 84, 387, 438, 452
FokI GGATG 3 cut(s) 734, 755, 866
FriOI GRGCYC 3 cut(s) 273, 414, 683
Fsp4HI GCNGC 5 cut(s) 43, 84, 387, 438, 452
FspBI CTAG 4 cut(s) 20, 242, 624, 840
GlaI GCGC 4 cut(s) 37, 92, 385, 547
GluI GCNGC 5 cut(s) 43, 84, 387, 438, 452
HaeIII GGCC 3 cut(s) 27, 42, 54
HapII CCGG 4 cut(s) 39, 189, 740, 828
HgaI GACGC 2 cut(s) 215, 274
HhaI GCGC 4 cut(s) 38, 93, 386, 548
Hin1I GRCGYC 1 cut(s) 207
Hin1II CATG 1 cut(s) 898
Hin6I GCGC 4 cut(s) 36, 91, 384, 546
HinP1I GCGC 4 cut(s) 36, 91, 384, 546
HindIII AAGCTT 1 cut(s) 293
HinfI GANTC 7 cut(s) 143, 155, 245, 368, 602, 803, 824
HpaII CCGG 4 cut(s) 39, 189, 740, 828
HphI GGTGA 7 cut(s) 142, 332, 482, 493, 619, 646, 812
Hpy166II GTNNAC 2 cut(s) 511, 637
Hpy188I TCNGA 4 cut(s) 337, 540, 567, 922
Hpy188III TCNNGA 4 cut(s) 62, 76, 740, 938
Hpy8I GTNNAC 2 cut(s) 511, 637
Hpy99I CGWCG 4 cut(s) 209, 269, 368, 836
HpyAV CCTTC 3 cut(s) 56, 524, 668
HpyCH4III ACNGT 2 cut(s) 757, 836
HpyCH4V TGCA 2 cut(s) 120, 725
HpyF10VI GCNNNNNNNGC 7 cut(s) 33, 42, 51, 215, 358, 409, 418
HpyF3I CTNAG 1 cut(s) 564
Hsp92I GRCGYC 1 cut(s) 207
Hsp92II CATG 1 cut(s) 898
HspAI GCGC 4 cut(s) 36, 91, 384, 546
Kpn2I TCCGGA 1 cut(s) 739
KroI GCCGGC 1 cut(s) 38
KroNI GCCGGC 1 cut(s) 40
KspI CCGCGG 2 cut(s) 319, 455
Kzo9I GATC 8 cut(s) 169, 324, 337, 390, 446, 540, 922, 926
LmnI GCTCC 5 cut(s) 400, 678, 686, 818, 913
LweI GCATC 2 cut(s) 201, 712
MaeI CTAG 4 cut(s) 20, 242, 624, 840
MaeIII GTNAC 2 cut(s) 148, 500
MalI GATC 8 cut(s) 171, 326, 339, 392, 448, 542, 924, 928
MboI GATC 8 cut(s) 169, 324, 337, 390, 446, 540, 922, 926
MboII GAAGA 2 cut(s) 280, 304
MflI RGATCY 1 cut(s) 540
MhlI GDGCHC 4 cut(s) 273, 414, 683, 918
MluCI AATT 7 cut(s) 344, 650, 664, 700, 776, 877, 931
MlyI GAGTC 4 cut(s) 137, 149, 377, 812
MmeI TCCRAC 1 cut(s) 627
MroI TCCGGA 1 cut(s) 739
MroNI GCCGGC 1 cut(s) 38
MseI TTAA 1 cut(s) 873
MspA1I CMGCKG 2 cut(s) 318, 454
MspI CCGG 4 cut(s) 39, 189, 740, 828
MspR9I CCNGG 2 cut(s) 88, 768
MvaI CCWGG 2 cut(s) 88, 768
MvnI CGCG 5 cut(s) 36, 318, 386, 454, 468
MwoI GCNNNNNNNGC 7 cut(s) 33, 42, 51, 215, 358, 409, 418
NaeI GCCGGC 1 cut(s) 40
NdeII GATC 8 cut(s) 169, 324, 337, 390, 446, 540, 922, 926
NgoMIV GCCGGC 1 cut(s) 38
NlaIII CATG 1 cut(s) 898
NlaIV GGNNCC 5 cut(s) 28, 55, 402, 532, 772
NmeAIII GCCGAG 3 cut(s) 30, 235, 393
NmuCI GTSAC 1 cut(s) 148
PcsI WCGNNNNNNNCGW 4 cut(s) 138, 207, 333, 414
PdiI GCCGGC 1 cut(s) 40
PfeI GAWTC 3 cut(s) 245, 602, 824
PflFI GACNNNGTC 2 cut(s) 146, 284
PkrI GCNGC 5 cut(s) 44, 85, 388, 439, 453
PleI GAGTC 4 cut(s) 137, 149, 376, 811
PpsI GAGTC 4 cut(s) 137, 149, 376, 811
PpuMI RGGWCCY 1 cut(s) 770
Psp124BI GAGCTC 2 cut(s) 414, 683
Psp5II RGGWCCY 1 cut(s) 770
Psp6I CCWGG 2 cut(s) 86, 766
PspGI CCWGG 2 cut(s) 86, 766
PspN4I GGNNCC 5 cut(s) 28, 55, 402, 532, 772
PspPI GGNCC 5 cut(s) 26, 53, 531, 737, 770
PspPPI RGGWCCY 1 cut(s) 770
PsuI RGATCY 1 cut(s) 540
PsyI GACNNNGTC 2 cut(s) 146, 284
RsaI GTAC 1 cut(s) 512
RsaNI GTAC 1 cut(s) 511
Rsr2I CGGWCCG 1 cut(s) 737
RsrII CGGWCCG 1 cut(s) 737
SacI GAGCTC 2 cut(s) 414, 683
SacII CCGCGG 2 cut(s) 319, 455
SaqAI TTAA 1 cut(s) 873
SatI GCNGC 5 cut(s) 43, 84, 387, 438, 452
Sau3AI GATC 8 cut(s) 169, 324, 337, 390, 446, 540, 922, 926
Sau96I GGNCC 5 cut(s) 26, 53, 531, 737, 770
SchI GAGTC 4 cut(s) 137, 149, 377, 812
ScrFI CCNGG 2 cut(s) 88, 768
SduI GDGCHC 4 cut(s) 273, 414, 683, 918
SfaNI GCATC 2 cut(s) 201, 712
Sfr303I CCGCGG 2 cut(s) 319, 455
SgrBI CCGCGG 2 cut(s) 319, 455
SinI GGWCC 3 cut(s) 531, 737, 770
SmlI CTYRAG 1 cut(s) 938
SmoI CTYRAG 1 cut(s) 938
Sse9I AATT 7 cut(s) 344, 650, 664, 700, 776, 877, 931
SspI AATATT 1 cut(s) 487
SspMI CTAG 4 cut(s) 20, 242, 624, 840
SstI GAGCTC 2 cut(s) 414, 683
StyD4I CCNGG 2 cut(s) 86, 766
StyI CCWWGG 2 cut(s) 19, 258
TaaI ACNGT 2 cut(s) 757, 836
TasI AATT 7 cut(s) 344, 650, 664, 700, 776, 877, 931
TauI GCSGC 5 cut(s) 45, 86, 389, 440, 454
TfiI GAWTC 3 cut(s) 245, 602, 824
Tru1I TTAA 1 cut(s) 873
Tru9I TTAA 1 cut(s) 873
TscAI CASTG 2 cut(s) 661, 802
TseFI GTSAC 1 cut(s) 148
Tsp45I GTSAC 1 cut(s) 148
TspDTI ATGAA 1 cut(s) 911
TspGWI ACGGA 1 cut(s) 136
TspRI CASTG 2 cut(s) 661, 802
Tth111I GACNNNGTC 2 cut(s) 146, 284
VpaK11BI GGWCC 3 cut(s) 531, 737, 770
XapI RAATTY 6 cut(s) 344, 650, 664, 700, 877, 931
XcmI CCANNNNNNNNNTGG 2 cut(s) 20, 556
XmaJI CCTAGG 1 cut(s) 19
XspI CTAG 4 cut(s) 20, 242, 624, 840
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.