pycom10g12330

Brefeldin A-inhibited guanine nucleotide-exchange protein

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
pyrus_communis
Chr10
OG0002933
Physical Location & Seq
Forward (+)
15848611 .. 15854647
6037 bp
Loading structure...
UTR
Exon/CDS
Intron
N/A

Sequence Viewer

Length: 2967 bp
ATGCTTGTCCTACGTGTGCTTGAGAATGTTCTCCAGCCTAGTTTCTTGCAAAAAATGACAGTCCTCAATCTATTGGAGAAGATCTCTCAGGATTCGCAGATTATCATTGACATTTTTGTCAACTATGACTGTGATGTCGATGCCCCAAACATCTTTGAAAGGATTGTCAATGGCCTTCTGAAAACTGCTCTAGGACCTCCCACTGGTTCAACAACAACATTGTCTCCAGTCCAAGATATCACTTTCCGGCATGAATCCGTAAAGTGCTTGGTTAGCATCATAAATTCAATGGGATCTTGGATGGACCAACAGCTGAGTATGGGAGACTCCTATCTACCAAAGACCAATGAAAGCGACACCTCAACTGAGAAAACAGAAAATAGTTCGACCCCAAATGGTGAAGAAGGTGCTGCTTTTGATAATGAAGTACATCCAGAAGGAAGTGCTGAAGTTTCAGATGCTGCAACTCTTGAGCAACGTCGAGCTTATAAGCTTGAACTTCAGAAAGGTGTCTCGCTGTTTAATAGGAAGCCTAACAAGGGCATTGAGTTTCTGATAAGCACCAAAAAAGTTGGTAGTTCCCCAGAAGATGTAGCTTCTTTCCTGAGGAACAACACTGCCGGCCTAAATGAAACCATGATTGGTGATTATTTGGGTGAAAGGGAGGAATTTCCTATAAAAGTTATGCATGCTTATGTTGATTCCTTTAATTTCAAAGGGATGGATTTCGGTGAAGCAATAAGGTTTTTCCTGCGGGGCTTCAGGTTACCTGGAGAAGCACAGAAAATTGACCGCATCATGGAGAAATTTGCTGAGCGCTACTGTAAATGCAGTCCAAATTCGTTTACAAGTGCAGATACTGCATATGTCCTTGCATACTCTGTGATAATGCTCAATACAGATGCCCATAACAACATGGTGAAAGATAAGATGACCAAGGCTGACTTCATCCGGAACAACCGAGGAATAGATGATGGAAAGGATCGATCTGTGGAGTATCTTGGTGTCCTGTATGATCAAATTGTTAAAAATGAGATTAAGATGAGCGCCGACTCTTCTGTACCACAGAGCAAGCAGGAAAACAGTTTTAATAAATTATTGGGCTTGGATGGTATCCTGAATTTGGTAACTGGGAAGCAGACTGAAGAAAAAGCATTGGGTGCAAATGGTCTTCTTATAAAGCACATTCAAGAGCAATTTAAAGCAAAGTCAGGAAAATCAGAGTCTGTCTATCATGCTGTGACAGATGTGGCAATTTTGAGGTTTATGGTGGAGGTCTGCTGGGGTCCTATGTTGGCTGCATTTAGTGTGACTCTTGACCAAAGTGATGACAGGCTTGCTACTTCTCAATGCTTACAAGGTTTTCGGCATGCTGTTCATGTTACTGCTTTGATGGGAATGCAGACCCAGAGAGATGCATTTGTCACTTCAGTAGCAAAGTTTACTTATCTACATAATGCTGCAGATATGAGGCAAAAAAATGTTGATGCTGTGAAGGCAATAATATCAATTGCCATTGAAGATGGTAATCATCTTCAGGAAGCATGGGAACACATATTAACGTGTCTTTCCCGAATCGAGCATTTGCAACTGTTGGGAGAAGGTGCACCCACTGATGCATCCTTTTTTACTGGATCAAAGGTCGAAACAGAGGAAAAATCACCAAAGCCTACTGGACTTTCTTCTCTGACAAAAAAGGGAACTATACAGAATCCAGCTGTGATGGCTGTTGTTCGTGGGGGTTCATATGACAGTACTACTGTCCGGGCGAACACTTCTGGACTGGTAACTCCAGAACAGATTAATAACTTCATTTCAAACTTGAATTTGCTGGATCAGATTGGGAATTTTGAGTTGAATCATGTATTTGCTCATAGCCAAAGATTAAACAGTGAAGCAATAGTGGCTTTTGTGAAGGCCTTGTGCAAAGTTTCCATGGCAGAGTTGCAATCTCCAACAGATCCTCGCGTATTCAGCCTCACAAAAATAGTTGAAATAGCGCATTACAATATGAACCGCATCAGATTAGTGTGGTCTCGCATATGGAATGTTCTCTCAGATTTCTTTGTATCAGTTGGCTTGTCAGAGAACCTCTCAGTTGCAATATTTGTGATGGATTCATTACGTCAGCTTGCTATGAAATTCTTAGAGCGCGAGGAGCTGGCAAATTACAACTTCCAGAATGAATTTTTGAGACCATTTGTGATTGTTATGCAAAAAAGCAACTCTACAGAAATTAGGGAATTAATTGTTCGGTGCATTTCACAAATGGTCCTCAGCCGTGTCAATCATGTGAAATCTGGCTGGAAAAGTGTATTTCTGGTTTTTACGGCAGCGGCAGCTGATGAGCGGAAAAATATTGTCTTGTTGGCCTTTGAGACCATGGAAAAAATCGTCAGAGAATACTTTCCCTATATAACAGAGACAGAGACAATGACTTTTACTGATTGTGTTAGATGCCTCTTAACCTTCACGAATAGCAGATTCAATAGTGATGTTAGCCTCAACGCTATTGCATTTCTCCGATACTGTGCTGTCAAACTTGCTGAAGGAGGGCTTGTTTACAACAAGAGGAGTGAGCTTGATGTTTCATCCCTTCCAACAGCAAATGAGGATGCTTCAAATGGAGTGACTTTCAATGAGAAAGATGAACATGCATCCTTTTGGGTTCCATTGCTAACAGGTTTGTCAAAGCTAACATCAGATCCTAGATCAGCAATCAGGAAGGGTTCTTTGGAAGTTCTTTTCAACATTCTAAAGGATCATGGTCATCTCTTCTCACGCTCCTTTTGGACTGCGATATTCAATTCTATTGTTTACCCCATATTTAGCTATGTCTGCGGTAAGAAAGACACACATATGGAAAAAGACCAGTCTTCGCCAGTTTCAGTATCTCCGCGTCCTGATGGAAGCACGTGGGATTCTGAAACTTCTGCAGTTGCAGCAGACTGTTTCTATGGTACATGA
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
GO:0000139 GO:0003674 GO:0005085 GO:0005086 GO:0005488 GO:0005515 GO:0005575 GO:0005622 GO:0005623 GO:0005634 GO:0005654 GO:0005730 GO:0005737 GO:0005794 GO:0005802 GO:0005829 GO:0006810 GO:0006887 GO:0006996 GO:0007030 GO:0008064 GO:0008092 GO:0008150 GO:0009507 GO:0009536 GO:0009889 GO:0009891 GO:0009893 GO:0009987 GO:0010256 GO:0010556 GO:0010557 GO:0010559 GO:0010560 GO:0010604 GO:0010639 GO:0012505 GO:0016020 GO:0016043 GO:0016192 GO:0016363 GO:0017022 GO:0019222 GO:0019899 GO:0030532 GO:0030832 GO:0030833 GO:0030837 GO:0031090 GO:0031323 GO:0031325 GO:0031326 GO:0031328 GO:0031333 GO:0031399 GO:0031401 GO:0031974 GO:0031981 GO:0031984 GO:0032268 GO:0032270 GO:0032271 GO:0032272 GO:0032535 GO:0032588 GO:0032878 GO:0032940 GO:0032956 GO:0032970 GO:0032991 GO:0033043 GO:0034237 GO:0034260 GO:0034399 GO:0043086 GO:0043087 GO:0043226 GO:0043227 GO:0043228 GO:0043229 GO:0043231 GO:0043232 GO:0043233 GO:0043254 GO:0044087 GO:0044092 GO:0044422 GO:0044424 GO:0044428 GO:0044431 GO:0044444 GO:0044446 GO:0044464 GO:0046903 GO:0048471 GO:0048518 GO:0048519 GO:0048522 GO:0048523 GO:0048583 GO:0048584 GO:0050789 GO:0050790 GO:0050794 GO:0051018 GO:0051020 GO:0051128 GO:0051129 GO:0051171 GO:0051173 GO:0051179 GO:0051234 GO:0051246 GO:0051247 GO:0051336 GO:0051346 GO:0051493 GO:0051494 GO:0060049 GO:0060050 GO:0060255 GO:0061041 GO:0065007 GO:0065008 GO:0065009 GO:0070013 GO:0071840 GO:0080090 GO:0080134 GO:0090066 GO:0090283 GO:0090284 GO:0090303 GO:0098588 GO:0098772 GO:0098791 GO:0110053 GO:0120114 GO:1902903 GO:1902904 GO:1903018 GO:1903020 GO:1903034 GO:1903036 GO:1990904 GO:2000112 GO:2000114
KEGG Pathways
Metabolic & Signaling
ko04144 map04144
Pfam Domains
Protein Families
DCB DUF1981 Mon2_C Sec7 Sec7_N

Protein Analysis

989

Amino Acids

110.34

Weight (kDa)

5.74

Isoelectric Point (pI)

38.35

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
Sec7-like_HUS PF12783 2 - 51 3.4e-09 Mon2/Sec7/BIG1-like, HUS domain
Sec7 PF01369 164 - 346 8.2e-74 Sec7 domain
Sec7-like_HDS PF09324 714 - 796 6.8e-30 Mon2/Sec7/BIG1-like, HDS
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AanI TTATAA 2 cut(s) 489, 1178
AasI GACNNNNNNGTC 2 cut(s) 116, 134
AccBSI CCGCTC 1 cut(s) 2350
AccII CGCG 3 cut(s) 1968, 2154, 2899
AccIII TCCGGA 1 cut(s) 951
AciI CCGC 7 cut(s) 754, 793, 2017, 2336, 2350, 2841, 2897
AclWI GGATC 7 cut(s) 301, 990, 1642, 1842, 1955, 2699, 2769
AcsI RAATTY 9 cut(s) 283, 668, 806, 838, 1120, 1825, 1846, 2141, 2186
AcuI CTGAAG 7 cut(s) 468, 485, 745, 1164, 1413, 1520, 2568
AcvI CACGTG 1 cut(s) 2916
AfaI GTAC 4 cut(s) 429, 1062, 1756, 2962
AfeI AGCGCT 1 cut(s) 818
AfiI CCNNNNNNNGG 4 cut(s) 203, 539, 799, 1123
AflIII ACRYGT 2 cut(s) 13, 1562
AjnI CCWGG 1 cut(s) 769
AjuI GAANNNNNNNTTGG 6 cut(s) 624, 656, 929, 961, 2717, 2749
Alw21I GWGCWC 1 cut(s) 1609
Alw26I GTCTC 8 cut(s) 228, 318, 517, 2040, 2188, 2372, 2417, 2423
Alw44I GTGCAC 1 cut(s) 1605
AlwI GGATC 7 cut(s) 301, 990, 1642, 1842, 1955, 2699, 2769
AlwNI CAGNNNCTG 3 cut(s) 461, 860, 1226
Aor13HI TCCGGA 1 cut(s) 951
Aor51HI AGCGCT 1 cut(s) 818
AoxI GGCC 4 cut(s) 172, 622, 1917, 2370
ApaLI GTGCAC 1 cut(s) 1605
ApeKI GCWGC 7 cut(s) 410, 461, 1298, 1460, 2333, 2339, 2942
ApoI RAATTY 9 cut(s) 283, 668, 806, 838, 1120, 1825, 1846, 2141, 2186
AseI ATTAAT 2 cut(s) 1803, 2246
Asp700I GAANNNNTTC 1 cut(s) 2406
AspLEI GCGC 4 cut(s) 819, 1049, 2002, 2154
AspS9I GGNCC 4 cut(s) 194, 304, 1286, 2272
AsuC2I CCSGG 1 cut(s) 1766
AsuHPI GGTGA 6 cut(s) 410, 656, 668, 743, 931, 1653
AvaII GGWCC 4 cut(s) 194, 304, 1286, 2272
AxyI CCTNAGG 1 cut(s) 605
BaeGI GKGCMC 1 cut(s) 1609
BbrPI CACGTG 1 cut(s) 2916
BbsI GAAGAC 2 cut(s) 1163, 2868
Bbv12I GWGCWC 1 cut(s) 1609
BbvCI CCTCAGC 1 cut(s) 2276
BbvI GCAGC 7 cut(s) 397, 448, 1285, 1447, 2345, 2351, 2954
BccI CCATC 9 cut(s) 295, 715, 968, 1103, 1387, 1517, 1717, 2107, 2900
BceAI ACGGC 2 cut(s) 2265, 2346
BciT130I CCWGG 1 cut(s) 771
BciVI GTATCC 1 cut(s) 1124
BclI TGATCA 1 cut(s) 1015
BcnI CCSGG 1 cut(s) 1766
BcoDI GTCTC 8 cut(s) 228, 318, 517, 2040, 2188, 2372, 2417, 2423
BfaI CTAG 3 cut(s) 39, 191, 2709
BfmI CTRYAG 3 cut(s) 1461, 2229, 2934
BfoI RGCGCY 2 cut(s) 820, 1050
BfuI GTATCC 1 cut(s) 1124
BglII AGATCT 1 cut(s) 81
BisI GCNGC 8 cut(s) 411, 462, 1299, 1461, 2334, 2337, 2340, 2943
BlpI GCTNAGC 1 cut(s) 813
BlsI GCNGC 8 cut(s) 412, 463, 1300, 1462, 2335, 2338, 2341, 2944
BmcAI AGTACT 1 cut(s) 1756
Bme1390I CCNGG 2 cut(s) 771, 1766
Bme18I GGWCC 4 cut(s) 194, 304, 1286, 2272
BmgT120I GGNCC 4 cut(s) 194, 304, 1286, 2272
BmiI GGNNCC 2 cut(s) 1287, 2670
BmrFI CCNGG 2 cut(s) 771, 1766
BmrI ACTGGG 1 cut(s) 1140
BmuI ACTGGG 1 cut(s) 1140
BpiI GAAGAC 2 cut(s) 1163, 2868
BplI GAGNNNNNCTC 4 cut(s) 68, 100, 2078, 2110
BpmI CTGGAG 4 cut(s) 17, 210, 792, 1776
Bpu10I CCTNAGC 1 cut(s) 2276
Bpu1102I GCTNAGC 1 cut(s) 813
BpuEI CTTGAG 2 cut(s) 41, 491
BpuMI CCSGG 1 cut(s) 1766
Bsa29I ATCGAT 1 cut(s) 985
BsaAI YACGTR 2 cut(s) 14, 2916
BsaBI GATNNNNATC 1 cut(s) 1527
BsaI GGTCTC 3 cut(s) 2040, 2188, 2372
BsaJI CCNNGG 4 cut(s) 936, 961, 1935, 2382
BsaWI WCCGGW 1 cut(s) 951
BsaXI ACNNNNNCTCC 4 cut(s) 208, 238, 2544, 2574
Bsc4I CCNNNNNNNGG 4 cut(s) 203, 539, 799, 1123
Bse118I RCCGGY 1 cut(s) 620
Bse1I ACTGG 8 cut(s) 208, 227, 1135, 1636, 1678, 1788, 2872, 2882
Bse21I CCTNAGG 1 cut(s) 605
Bse3DI GCAATG 1 cut(s) 2672
Bse8I GATNNNNATC 1 cut(s) 1527
BseAI TCCGGA 1 cut(s) 951
BseBI CCWGG 1 cut(s) 771
BseCI ATCGAT 1 cut(s) 985
BseDI CCNNGG 4 cut(s) 936, 961, 1935, 2382
BseGI GGATG 9 cut(s) 306, 430, 726, 948, 1114, 1619, 2591, 2620, 2657
BseJI GATNNNNATC 1 cut(s) 1527
BseLI CCNNNNNNNGG 4 cut(s) 203, 539, 799, 1123
BseMI GCAATG 1 cut(s) 2672
BseMII CTCAG 8 cut(s) 101, 305, 357, 596, 804, 2070, 2109, 2290
BseNI ACTGG 8 cut(s) 208, 227, 1135, 1636, 1678, 1788, 2872, 2882
BseRI GAGGAG 2 cut(s) 2171, 2587
BseSI GKGCMC 1 cut(s) 1609
BseXI GCAGC 7 cut(s) 397, 448, 1285, 1447, 2345, 2351, 2954
BseYI CCCAGC 1 cut(s) 1281
BsgI GTGCAG 1 cut(s) 873
Bsh1236I CGCG 3 cut(s) 1968, 2154, 2899
BshFI GGCC 4 cut(s) 174, 624, 1919, 2372
BshVI ATCGAT 1 cut(s) 985
BsiHKAI GWGCWC 1 cut(s) 1609
BsiSI CCGG 4 cut(s) 247, 621, 952, 1765
BslI CCNNNNNNNGG 4 cut(s) 203, 539, 799, 1123
BsmAI GTCTC 8 cut(s) 228, 318, 517, 2040, 2188, 2372, 2417, 2423
BsmI GAATGC 1 cut(s) 1404
BsnI GGCC 4 cut(s) 174, 624, 1919, 2372
Bso31I GGTCTC 3 cut(s) 2040, 2188, 2372
Bsp1286I GDGCHC 1 cut(s) 1609
Bsp13I TCCGGA 1 cut(s) 951
Bsp1720I GCTNAGC 1 cut(s) 813
Bsp19I CCATGG 2 cut(s) 1935, 2382
BspACI CCGC 7 cut(s) 754, 793, 2017, 2336, 2350, 2841, 2897
BspANI GGCC 4 cut(s) 174, 624, 1919, 2372
BspCNI CTCAG 8 cut(s) 100, 306, 358, 597, 805, 2069, 2108, 2289
BspDI ATCGAT 1 cut(s) 985
BspEI TCCGGA 1 cut(s) 951
BspFNI CGCG 3 cut(s) 1968, 2154, 2899
BspLI GGNNCC 2 cut(s) 1287, 2670
BspMAI CTGCAG 2 cut(s) 1465, 2938
BspPI GGATC 7 cut(s) 301, 990, 1642, 1842, 1955, 2699, 2769
BspTNI GGTCTC 3 cut(s) 2040, 2188, 2372
BsrBI CCGCTC 1 cut(s) 2350
BsrDI GCAATG 1 cut(s) 2672
BsrFI RCCGGY 1 cut(s) 620
BsrI ACTGG 8 cut(s) 208, 227, 1135, 1636, 1678, 1788, 2872, 2882
BssAI RCCGGY 1 cut(s) 620
BssECI CCNNGG 4 cut(s) 936, 961, 1935, 2382
BssT1I CCWWGG 3 cut(s) 936, 1935, 2382
Bst2UI CCWGG 1 cut(s) 771
Bst6I CTCTTC 2 cut(s) 1060, 2780
BstAPI GCANNNNNTGC 2 cut(s) 860, 1160
BstBAI YACGTR 2 cut(s) 14, 2916
BstC8I GCNNGC 7 cut(s) 622, 690, 1073, 1338, 1371, 2133, 2163
BstDEI CTNAG 9 cut(s) 87, 314, 366, 605, 813, 2056, 2095, 2146, 2276
BstDSI CCRYGG 2 cut(s) 1935, 2382
BstEII GGTNACC 1 cut(s) 765
BstF5I GGATG 9 cut(s) 306, 430, 726, 948, 1114, 1619, 2591, 2620, 2657
BstFNI CGCG 3 cut(s) 1968, 2154, 2899
BstH2I RGCGCY 2 cut(s) 820, 1050
BstHHI GCGC 4 cut(s) 819, 1049, 2002, 2154
BstMAI GTCTC 8 cut(s) 228, 318, 517, 2040, 2188, 2372, 2417, 2423
BstNI CCWGG 1 cut(s) 771
BstNSI RCATGY 3 cut(s) 692, 1373, 2657
BstPI GGTNACC 1 cut(s) 765
BstSCI CCNGG 2 cut(s) 769, 1764
BstSFI CTRYAG 3 cut(s) 1461, 2229, 2934
BstSLI GKGCMC 1 cut(s) 1609
BstUI CGCG 3 cut(s) 1968, 2154, 2899
BstV1I GCAGC 7 cut(s) 397, 448, 1285, 1447, 2345, 2351, 2954
BstV2I GAAGAC 2 cut(s) 1163, 2868
BstX2I RGATCY 4 cut(s) 81, 293, 1960, 2704
BstYI RGATCY 4 cut(s) 81, 293, 1960, 2704
Bsu15I ATCGAT 1 cut(s) 985
Bsu36I CCTNAGG 1 cut(s) 605
BsuI GTATCC 1 cut(s) 1124
BsuRI GGCC 4 cut(s) 174, 624, 1919, 2372
BsuTUI ATCGAT 1 cut(s) 985
BtgI CCRYGG 2 cut(s) 1935, 2382
BtsCI GGATG 9 cut(s) 306, 430, 726, 948, 1114, 1619, 2591, 2620, 2657
BtsI GCAGTG 1 cut(s) 615
BtsIMutI CAGTG 4 cut(s) 201, 615, 1611, 1897
Cac8I GCNNGC 7 cut(s) 622, 690, 1073, 1338, 1371, 2133, 2163
CaiI CAGNNNCTG 3 cut(s) 461, 860, 1226
CfoI GCGC 4 cut(s) 819, 1049, 2002, 2154
Cfr10I RCCGGY 1 cut(s) 620
Cfr13I GGNCC 4 cut(s) 194, 304, 1286, 2272
ClaI ATCGAT 1 cut(s) 985
CseI GACGC 1 cut(s) 2888
Csp6I GTAC 4 cut(s) 428, 1061, 1755, 2961
CviQI GTAC 4 cut(s) 428, 1061, 1755, 2961
DdeI CTNAG 9 cut(s) 87, 314, 366, 605, 813, 2056, 2095, 2146, 2276
DraI TTTAAA 1 cut(s) 1201
DrdI GACNNNNNNGTC 2 cut(s) 116, 134
DseDI GACNNNNNNGTC 2 cut(s) 116, 134
Eam1104I CTCTTC 2 cut(s) 1060, 2780
EarI CTCTTC 2 cut(s) 1060, 2780
Eco130I CCWWGG 3 cut(s) 936, 1935, 2382
Eco147I AGGCCT 1 cut(s) 1919
Eco31I GGTCTC 3 cut(s) 2040, 2188, 2372
Eco32I GATATC 1 cut(s) 238
Eco47I GGWCC 4 cut(s) 194, 304, 1286, 2272
Eco47III AGCGCT 1 cut(s) 818
Eco57I CTGAAG 7 cut(s) 468, 485, 745, 1164, 1413, 1520, 2568
Eco72I CACGTG 1 cut(s) 2916
Eco81I CCTNAGG 1 cut(s) 605
Eco91I GGTNACC 1 cut(s) 765
EcoO109I RGGNCCY 2 cut(s) 194, 1286
EcoO65I GGTNACC 1 cut(s) 765
EcoRII CCWGG 1 cut(s) 769
EcoRV GATATC 1 cut(s) 238
EcoT14I CCWWGG 3 cut(s) 936, 1935, 2382
EcoT22I ATGCAT 4 cut(s) 690, 1420, 1621, 2659
ErhI CCWWGG 3 cut(s) 936, 1935, 2382
FalI AAGNNNNNCTT 4 cut(s) 929, 961, 2541, 2573
FauI CCCGC 1 cut(s) 747
FauNDI CATATG 4 cut(s) 865, 1747, 2042, 2859
FbaI TGATCA 1 cut(s) 1015
Fnu4HI GCNGC 8 cut(s) 411, 462, 1299, 1461, 2334, 2337, 2340, 2943
FokI GGATG 9 cut(s) 313, 417, 733, 935, 1121, 1606, 2578, 2627, 2644
Fsp4HI GCNGC 8 cut(s) 411, 462, 1299, 1461, 2334, 2337, 2340, 2943
FspBI CTAG 3 cut(s) 39, 191, 2709
GlaI GCGC 4 cut(s) 818, 1048, 2001, 2153
GluI GCNGC 8 cut(s) 411, 462, 1299, 1461, 2334, 2337, 2340, 2943
GsaI CCCAGC 1 cut(s) 1285
GsuI CTGGAG 4 cut(s) 17, 210, 792, 1776
HaeII RGCGCY 2 cut(s) 820, 1050
HaeIII GGCC 4 cut(s) 174, 624, 1919, 2372
HapII CCGG 4 cut(s) 247, 621, 952, 1765
HgaI GACGC 1 cut(s) 2888
HhaI GCGC 4 cut(s) 819, 1049, 2002, 2154
Hin6I GCGC 4 cut(s) 817, 1047, 2000, 2152
HinP1I GCGC 4 cut(s) 817, 1047, 2000, 2152
HincII GTYRAC 1 cut(s) 121
HindII GTYRAC 1 cut(s) 121
HindIII AAGCTT 1 cut(s) 491
HpaII CCGG 4 cut(s) 247, 621, 952, 1765
HphI GGTGA 6 cut(s) 410, 656, 668, 743, 931, 1653
Hpy166II GTNNAC 6 cut(s) 121, 846, 1443, 1607, 2563, 2818
Hpy8I GTNNAC 6 cut(s) 121, 846, 1443, 1607, 2563, 2818
Hpy99I CGWCG 1 cut(s) 483
HpyCH4IV ACGT 5 cut(s) 13, 478, 1562, 2125, 2915
HpyF3I CTNAG 9 cut(s) 87, 314, 366, 605, 813, 2056, 2095, 2146, 2276
HpySE526I ACGT 5 cut(s) 13, 478, 1562, 2125, 2915
HspAI GCGC 4 cut(s) 817, 1047, 2000, 2152
Kpn2I TCCGGA 1 cut(s) 951
KroI GCCGGC 1 cut(s) 620
KroNI GCCGGC 1 cut(s) 622
Ksp22I TGATCA 1 cut(s) 1015
LmnI GCTCC 2 cut(s) 2158, 2789
Lsp1109I GCAGC 7 cut(s) 397, 448, 1285, 1447, 2345, 2351, 2954
MaeI CTAG 3 cut(s) 39, 191, 2709
MaeII ACGT 5 cut(s) 13, 478, 1562, 2125, 2915
MaeIII GTNAC 8 cut(s) 765, 1126, 1240, 1309, 1381, 1423, 1786, 2629
MbiI CCGCTC 1 cut(s) 2350
MfeI CAATTG 1 cut(s) 1509
MflI RGATCY 4 cut(s) 81, 293, 1960, 2704
MhlI GDGCHC 1 cut(s) 1609
MlyI GAGTC 4 cut(s) 320, 1046, 1232, 1306
MmeI TCCRAC 2 cut(s) 1979, 2624
Mph1103I ATGCAT 4 cut(s) 690, 1420, 1621, 2659
MroI TCCGGA 1 cut(s) 951
MroNI GCCGGC 1 cut(s) 620
MroXI GAANNNNTTC 1 cut(s) 2406
MslI CAYNNNNRTG 2 cut(s) 693, 2858
MspA1I CMGCKG 4 cut(s) 313, 1718, 2336, 2342
MspI CCGG 4 cut(s) 247, 621, 952, 1765
MspR9I CCNGG 2 cut(s) 771, 1766
MunI CAATTG 1 cut(s) 1509
Mva1269I GAATGC 1 cut(s) 1404
MvaI CCWGG 1 cut(s) 771
MvnI CGCG 3 cut(s) 1968, 2154, 2899
NaeI GCCGGC 1 cut(s) 622
NciI CCSGG 1 cut(s) 1766
NcoI CCATGG 2 cut(s) 1935, 2382
NdeI CATATG 4 cut(s) 865, 1747, 2042, 2859
NgoMIV GCCGGC 1 cut(s) 620
NlaIV GGNNCC 2 cut(s) 1287, 2670
NmuCI GTSAC 4 cut(s) 1240, 1309, 1423, 2629
NsiI ATGCAT 4 cut(s) 690, 1420, 1621, 2659
NspI RCATGY 3 cut(s) 692, 1373, 2657
PaeI GCATGC 2 cut(s) 692, 1373
PceI AGGCCT 1 cut(s) 1919
PctI GAATGC 1 cut(s) 1404
PdiI GCCGGC 1 cut(s) 622
PdmI GAANNNNTTC 1 cut(s) 2406
PfeI GAWTC 9 cut(s) 92, 254, 701, 1575, 1711, 1858, 2117, 2484, 2921
PkrI GCNGC 8 cut(s) 412, 463, 1300, 1462, 2335, 2338, 2341, 2944
PleI GAGTC 4 cut(s) 320, 1046, 1231, 1306
PmaCI CACGTG 1 cut(s) 2916
PmlI CACGTG 1 cut(s) 2916
PpsI GAGTC 4 cut(s) 320, 1046, 1231, 1306
Ppu21I YACGTR 2 cut(s) 14, 2916
PpuMI RGGWCCY 2 cut(s) 194, 1286
PshBI ATTAAT 2 cut(s) 1803, 2246
PsiI TTATAA 2 cut(s) 489, 1178
Psp5II RGGWCCY 2 cut(s) 194, 1286
Psp6I CCWGG 1 cut(s) 769
PspCI CACGTG 1 cut(s) 2916
PspEI GGTNACC 1 cut(s) 765
PspFI CCCAGC 1 cut(s) 1281
PspGI CCWGG 1 cut(s) 769
PspN4I GGNNCC 2 cut(s) 1287, 2670
PspPI GGNCC 4 cut(s) 194, 304, 1286, 2272
PspPPI RGGWCCY 2 cut(s) 194, 1286
PstI CTGCAG 2 cut(s) 1465, 2938
PstNI CAGNNNCTG 3 cut(s) 461, 860, 1226
PsuI RGATCY 4 cut(s) 81, 293, 1960, 2704
PvuII CAGCTG 3 cut(s) 313, 1718, 2342
RsaI GTAC 4 cut(s) 429, 1062, 1756, 2962
RsaNI GTAC 4 cut(s) 428, 1061, 1755, 2961
RseI CAYNNNNRTG 2 cut(s) 693, 2858
SatI GCNGC 8 cut(s) 411, 462, 1299, 1461, 2334, 2337, 2340, 2943
Sau96I GGNCC 4 cut(s) 194, 304, 1286, 2272
ScaI AGTACT 1 cut(s) 1756
SchI GAGTC 4 cut(s) 320, 1046, 1232, 1306
ScrFI CCNGG 2 cut(s) 771, 1766
SduI GDGCHC 1 cut(s) 1609
SfcI CTRYAG 3 cut(s) 1461, 2229, 2934
SinI GGWCC 4 cut(s) 194, 304, 1286, 2272
SmiMI CAYNNNNRTG 2 cut(s) 693, 2858
SmlI CTYRAG 2 cut(s) 20, 470
SmoI CTYRAG 2 cut(s) 20, 470
SphI GCATGC 2 cut(s) 692, 1373
SseBI AGGCCT 1 cut(s) 1919
SsiI CCGC 7 cut(s) 754, 793, 2017, 2336, 2350, 2841, 2897
SspI AATATT 2 cut(s) 2106, 2359
SspMI CTAG 3 cut(s) 39, 191, 2709
StuI AGGCCT 1 cut(s) 1919
StyD4I CCNGG 2 cut(s) 769, 1764
StyI CCWWGG 3 cut(s) 936, 1935, 2382
TaiI ACGT 5 cut(s) 16, 481, 1565, 2128, 2918
TaqI TCGA 6 cut(s) 138, 386, 481, 985, 1578, 1644
TatI WGTACW 2 cut(s) 427, 1754
TauI GCSGC 1 cut(s) 2339
TfiI GAWTC 9 cut(s) 92, 254, 701, 1575, 1711, 1858, 2117, 2484, 2921
TscAI CASTG 4 cut(s) 208, 622, 1618, 1897
TseFI GTSAC 4 cut(s) 1240, 1309, 1423, 2629
TseI GCWGC 7 cut(s) 410, 461, 1298, 1460, 2333, 2339, 2942
Tsp45I GTSAC 4 cut(s) 1240, 1309, 1423, 2629
TspGWI ACGGA 1 cut(s) 247
TspRI CASTG 4 cut(s) 208, 622, 1618, 1897
VneI GTGCAC 1 cut(s) 1605
VpaK11BI GGWCC 4 cut(s) 194, 304, 1286, 2272
VspI ATTAAT 2 cut(s) 1803, 2246
XapI RAATTY 9 cut(s) 283, 668, 806, 838, 1120, 1825, 1846, 2141, 2186
XceI RCATGY 3 cut(s) 692, 1373, 2657
XmnI GAANNNNTTC 1 cut(s) 2406
XspI CTAG 3 cut(s) 39, 191, 2709
ZrmI AGTACT 1 cut(s) 1756
Zsp2I ATGCAT 4 cut(s) 690, 1420, 1621, 2659
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.