pycom10g20120

Cytochrome p450

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
pyrus_communis
Chr10
OG0002150
Physical Location & Seq
Reverse (-)
23090204 .. 23091774
1571 bp
Loading structure...
UTR
Exon/CDS
Intron
N/A

Sequence Viewer

Length: 1443 bp
ATGGAGACCCTTTACCTTATATTTTCTGTGGGTGCTGCTTTCTTAGCCCTTACGATCTTTGCACTCAAAGGCAAATCAGATGGTGCAAAAAACCTTCCACCAGGGAGCATGGGGTGGCCTATTGTGGGTGAATCAATCGAGTTCTTGTTCGGGAAGCCGGAAAATTTTGTGTTCAAGAGGATGAGGAAGTACTCTCCTGACATCTTCAAGACCTATATTCTTGGAGAGAAAACTGCTGTGATCTGTGGTCCTAGCGGACACAAATTTCTGTTCTCCAATGAACAAAAGTACTTCACAGCTTTTCGACCACATTCGATGCAAAAGATGTTTCGATCATACAAGGCTGCCGCCCCCACTGCTTCTGCTGCTCCCGCTGTGGCACAACCTTCTCGCGATGAAGAAGCGAAAGTTATAAGATCTCCGGGGTTTTTGAAGCCGGAAGCATTGGTGAGGTACTTGGGGAAAATGGACTCTATCACTCAGGAACAGATGAAGGCCTATTGGGAAGGCAAAGATGAGGTCAAGGTCTACCCTTTGGCCAAGACACTCACTCTAAGTCTTGCCTGCAGATTCTTCTTGGGGATCGATGATTCCGAGCGAATTGCAAGGCTGGTGAGCAATTTTGATGATGTTACCGTTGGGATGCATTCACTTATTATCAACTTTCCAGGAACAACATTCTATAAGGCAACCAAAGCAGCCGATGCGCTGCGAAAGGAGTTGAGGATTGTGATTCAGGAGAAGAAGGCTGCAATGGCCGCAGGAGGTCCCATGCATGATATATTGTCACATATGATCGTGGCTAGTGATCCATCCGGCAAACACATGCCCGAGGCTGAGGTTGCAGACAAGATCATGGGTTTATTGACTGCAGGATATAGCACTGTGGCCACTGCTATGACTTTCTTCATGAAATATGTTGGAGAGAGGCCAGACATTTATGCCAAGGTCCTAGCTGAGCACAAGGAGATTGCTGACTCGAAGAAGCCAGGAGACTTTTTGGAGTGGGAAGACATCAACAAAATGAAGTACTCATGGAATGTGTTGTATGAAGTCATGAGATTCACACCGCCACTTCAGGGGACATTCAGAGAGGCCTTGACAGATTTTACCTACGCCGGTTACACCATCCCGAAGGGCTGGAAGGTATATTGGACTGTGAGTACAACAAACATGAACCCACAGTACTTCCCTAACCCCGAAAAGTTTGATCCATCAAGATATGAGGACCTAAATGCATTCCCGGCATTCACACTGGTTCCATTTGGAGGAGGTCCCAGAATGTGCCCCGGGAAAGAGTACGCTCGCCTAGCGATACTCACTTTCGTTCACAATGTGGTGAAGAGGTTCGAATGGGAACTTGTGTTTCCCAAGGAGAAGATCACAGGTGATATGATGCCAACACCAGAGAAAGGGCTTCCAGTTCGCCTTCGACGTCACTAG
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
No pathways identified.
Pfam Domains
Protein Families
p450

Protein Analysis

481

Amino Acids

54.17

Weight (kDa)

9.19

Isoelectric Point (pI)

39.07

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
p450 PF00067 33 - 455 2.1e-58 Cytochrome P450
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Publication-ready
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Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AanI TTATAA 1 cut(s) 413
AatII GACGTC 1 cut(s) 1438
AccI GTMKAC 1 cut(s) 528
AccII CGCG 1 cut(s) 393
AciI CCGC 5 cut(s) 255, 348, 372, 759, 1070
AclWI GGATC 3 cut(s) 590, 803, 1205
AcoI YGGCCR 3 cut(s) 537, 756, 888
AcsI RAATTY 2 cut(s) 163, 263
AcuI CTGAAG 1 cut(s) 1061
AcyI GRCGYC 1 cut(s) 1435
AdeI CACNNNGTG 1 cut(s) 1336
AfaI GTAC 7 cut(s) 191, 290, 455, 1031, 1165, 1187, 1301
AfiI CCNNNNNNNGG 4 cut(s) 125, 1079, 1268, 1412
AgsI TTSAA 3 cut(s) 175, 208, 433
AjnI CCWGG 3 cut(s) 100, 667, 988
AluBI AGCT 2 cut(s) 299, 956
AluI AGCT 2 cut(s) 299, 956
Alw21I GWGCWC 1 cut(s) 963
Alw26I GTCTC 1 cut(s) 987
AlwI GGATC 3 cut(s) 590, 803, 1205
Ama87I CYCGRG 2 cut(s) 830, 1289
AoxI GGCC 7 cut(s) 116, 495, 537, 756, 888, 929, 1095
ApeKI GCWGC 6 cut(s) 35, 344, 365, 698, 709, 749
ApoI RAATTY 2 cut(s) 163, 263
ArsI GACNNNNNNTTYG 2 cut(s) 504, 536
Asp700I GAANNNNTTC 1 cut(s) 1346
AspLEI GCGC 1 cut(s) 709
AspS9I GGNCC 5 cut(s) 248, 767, 949, 1228, 1274
AsuC2I CCSGG 4 cut(s) 423, 1244, 1290, 1291
AsuHPI GGTGA 5 cut(s) 140, 460, 625, 1351, 1400
AsuII TTCGAA 1 cut(s) 1350
AvaI CYCGRG 2 cut(s) 830, 1289
AvaII GGWCC 5 cut(s) 248, 767, 949, 1228, 1274
BaeGI GKGCMC 1 cut(s) 1289
BalI TGGCCA 2 cut(s) 539, 890
BbsI GAAGAC 1 cut(s) 1017
Bbv12I GWGCWC 1 cut(s) 963
BbvCI CCTCAGC 1 cut(s) 837
BbvI GCAGC 6 cut(s) 22, 331, 352, 696, 710, 736
BccI CCATC 4 cut(s) 74, 820, 1136, 1222
BcgI CGANNNNNNTGC 2 cut(s) 584, 618
BciT130I CCWGG 3 cut(s) 102, 669, 990
BcnI CCSGG 4 cut(s) 423, 1244, 1290, 1291
BcoDI GTCTC 1 cut(s) 987
BfaI CTAG 5 cut(s) 252, 804, 953, 1310, 1441
BfmI CTRYAG 2 cut(s) 565, 870
BglII AGATCT 1 cut(s) 416
BisI GCNGC 8 cut(s) 36, 345, 348, 366, 699, 710, 750, 759
BlpI GCTNAGC 1 cut(s) 957
BlsI GCNGC 8 cut(s) 37, 346, 349, 367, 700, 711, 751, 760
BmcAI AGTACT 4 cut(s) 191, 290, 1031, 1187
Bme1390I CCNGG 7 cut(s) 102, 423, 669, 990, 1244, 1290, 1291
Bme18I GGWCC 5 cut(s) 248, 767, 949, 1228, 1274
BmeT110I CYCGRG 2 cut(s) 830, 1289
BmgT120I GGNCC 5 cut(s) 248, 767, 949, 1228, 1274
BmiI GGNNCC 3 cut(s) 769, 1260, 1276
BmrFI CCNGG 7 cut(s) 102, 423, 669, 990, 1244, 1290, 1291
BmsI GCATC 4 cut(s) 306, 633, 694, 1386
BpiI GAAGAC 1 cut(s) 1017
Bpu10I CCTNAGC 1 cut(s) 837
Bpu1102I GCTNAGC 1 cut(s) 957
Bpu14I TTCGAA 1 cut(s) 1350
BpuMI CCSGG 4 cut(s) 423, 1244, 1290, 1291
Bsa29I ATCGAT 1 cut(s) 585
BsaHI GRCGYC 1 cut(s) 1435
BsaJI CCNNGG 7 cut(s) 101, 422, 831, 945, 1288, 1289, 1371
Bsc4I CCNNNNNNNGG 4 cut(s) 125, 1079, 1268, 1412
Bse118I RCCGGY 1 cut(s) 1118
Bse1I ACTGG 2 cut(s) 1260, 1421
Bse3DI GCAATG 1 cut(s) 759
BseBI CCWGG 3 cut(s) 102, 669, 990
BseCI ATCGAT 1 cut(s) 585
BseDI CCNNGG 7 cut(s) 101, 422, 831, 945, 1288, 1289, 1371
BseGI GGATG 4 cut(s) 186, 648, 812, 1128
BseLI CCNNNNNNNGG 4 cut(s) 125, 1079, 1268, 1412
BseMI GCAATG 1 cut(s) 759
BseMII CTCAG 3 cut(s) 494, 828, 948
BseNI ACTGG 2 cut(s) 1260, 1421
BseRI GAGGAG 1 cut(s) 1284
BseSI GKGCMC 1 cut(s) 1289
BseXI GCAGC 6 cut(s) 22, 331, 352, 696, 710, 736
Bsh1236I CGCG 1 cut(s) 393
BshFI GGCC 7 cut(s) 118, 497, 539, 758, 890, 931, 1097
BshVI ATCGAT 1 cut(s) 585
BsiHKAI GWGCWC 1 cut(s) 963
BsiHKCI CYCGRG 2 cut(s) 830, 1289
BsiSI CCGG 7 cut(s) 158, 422, 437, 816, 1119, 1244, 1290
BslFI GGGAC 3 cut(s) 753, 1096, 1260
BslI CCNNNNNNNGG 4 cut(s) 125, 1079, 1268, 1412
BsmAI GTCTC 1 cut(s) 987
BsmFI GGGAC 3 cut(s) 753, 1096, 1260
BsmI GAATGC 3 cut(s) 646, 1238, 1247
BsnI GGCC 7 cut(s) 118, 497, 539, 758, 890, 931, 1097
BsoBI CYCGRG 2 cut(s) 830, 1289
Bsp119I TTCGAA 1 cut(s) 1350
Bsp1286I GDGCHC 2 cut(s) 963, 1289
Bsp1720I GCTNAGC 1 cut(s) 957
Bsp68I TCGCGA 1 cut(s) 393
BspACI CCGC 5 cut(s) 255, 348, 372, 759, 1070
BspANI GGCC 7 cut(s) 118, 497, 539, 758, 890, 931, 1097
BspCNI CTCAG 3 cut(s) 493, 829, 949
BspDI ATCGAT 1 cut(s) 585
BspFNI CGCG 1 cut(s) 393
BspHI TCATGA 2 cut(s) 909, 1056
BspLI GGNNCC 3 cut(s) 769, 1260, 1276
BspMAI CTGCAG 2 cut(s) 569, 874
BspPI GGATC 3 cut(s) 590, 803, 1205
BspT104I TTCGAA 1 cut(s) 1350
BsrDI GCAATG 1 cut(s) 759
BsrFI RCCGGY 1 cut(s) 1118
BsrI ACTGG 2 cut(s) 1260, 1421
BssAI RCCGGY 1 cut(s) 1118
BssECI CCNNGG 7 cut(s) 101, 422, 831, 945, 1288, 1289, 1371
BssNI GRCGYC 1 cut(s) 1435
BssT1I CCWWGG 2 cut(s) 945, 1371
Bst2UI CCWGG 3 cut(s) 102, 669, 990
Bst4CI ACNGT 4 cut(s) 637, 886, 1159, 1185
Bst6I CTCTTC 1 cut(s) 1337
BstACI GRCGYC 1 cut(s) 1435
BstAPI GCANNNNNTGC 1 cut(s) 704
BstBI TTCGAA 1 cut(s) 1350
BstC8I GCNNGC 2 cut(s) 565, 1306
BstDEI CTNAG 5 cut(s) 43, 480, 554, 837, 957
BstF5I GGATG 4 cut(s) 186, 648, 812, 1128
BstFNI CGCG 1 cut(s) 393
BstHHI GCGC 1 cut(s) 709
BstMAI GTCTC 1 cut(s) 987
BstNI CCWGG 3 cut(s) 102, 669, 990
BstNSI RCATGY 1 cut(s) 829
BstSCI CCNGG 7 cut(s) 100, 421, 667, 988, 1242, 1288, 1289
BstSFI CTRYAG 2 cut(s) 565, 870
BstSLI GKGCMC 1 cut(s) 1289
BstUI CGCG 1 cut(s) 393
BstV1I GCAGC 6 cut(s) 22, 331, 352, 696, 710, 736
BstV2I GAAGAC 1 cut(s) 1017
BstX2I RGATCY 1 cut(s) 416
BstYI RGATCY 1 cut(s) 416
Bsu15I ATCGAT 1 cut(s) 585
BsuRI GGCC 7 cut(s) 118, 497, 539, 758, 890, 931, 1097
BsuTUI ATCGAT 1 cut(s) 585
BtgZI GCGATG 1 cut(s) 408
BtsCI GGATG 4 cut(s) 186, 648, 812, 1128
BtsI GCAGTG 2 cut(s) 354, 891
BtsIMutI CAGTG 4 cut(s) 354, 882, 891, 1253
BtuMI TCGCGA 1 cut(s) 393
Cac8I GCNNGC 2 cut(s) 565, 1306
CciI TCATGA 2 cut(s) 909, 1056
CfoI GCGC 1 cut(s) 709
Cfr10I RCCGGY 1 cut(s) 1118
Cfr13I GGNCC 5 cut(s) 248, 767, 949, 1228, 1274
Cfr9I CCCGGG 1 cut(s) 1289
ClaI ATCGAT 1 cut(s) 585
Csp6I GTAC 7 cut(s) 190, 289, 454, 1030, 1164, 1186, 1300
CviAII CATG 9 cut(s) 109, 772, 776, 826, 856, 910, 1035, 1057, 1174
CviQI GTAC 7 cut(s) 190, 289, 454, 1030, 1164, 1186, 1300
DdeI CTNAG 5 cut(s) 43, 480, 554, 837, 957
DraIII CACNNNGTG 1 cut(s) 1336
EaeI YGGCCR 3 cut(s) 537, 756, 888
Eam1104I CTCTTC 1 cut(s) 1337
EarI CTCTTC 1 cut(s) 1337
Eco130I CCWWGG 2 cut(s) 945, 1371
Eco147I AGGCCT 2 cut(s) 497, 1097
Eco47I GGWCC 5 cut(s) 248, 767, 949, 1228, 1274
Eco57I CTGAAG 1 cut(s) 1061
Eco88I CYCGRG 2 cut(s) 830, 1289
EcoO109I RGGNCCY 4 cut(s) 767, 949, 1228, 1274
EcoRII CCWGG 3 cut(s) 100, 667, 988
EcoT14I CCWWGG 2 cut(s) 945, 1371
EcoT22I ATGCAT 3 cut(s) 648, 777, 1240
ErhI CCWWGG 2 cut(s) 945, 1371
FaeI CATG 9 cut(s) 112, 775, 779, 829, 859, 913, 1038, 1060, 1177
FaqI GGGAC 3 cut(s) 753, 1096, 1260
FatI CATG 9 cut(s) 108, 771, 775, 825, 855, 909, 1034, 1056, 1173
FauI CCCGC 1 cut(s) 379
FauNDI CATATG 1 cut(s) 792
FblI GTMKAC 1 cut(s) 528
Fnu4HI GCNGC 8 cut(s) 36, 345, 348, 366, 699, 710, 750, 759
FokI GGATG 4 cut(s) 193, 655, 799, 1115
Fsp4HI GCNGC 8 cut(s) 36, 345, 348, 366, 699, 710, 750, 759
FspBI CTAG 5 cut(s) 252, 804, 953, 1310, 1441
GlaI GCGC 1 cut(s) 708
GluI GCNGC 8 cut(s) 36, 345, 348, 366, 699, 710, 750, 759
HaeIII GGCC 7 cut(s) 118, 497, 539, 758, 890, 931, 1097
HapII CCGG 7 cut(s) 158, 422, 437, 816, 1119, 1244, 1290
HhaI GCGC 1 cut(s) 709
Hin1I GRCGYC 1 cut(s) 1435
Hin1II CATG 9 cut(s) 112, 775, 779, 829, 859, 913, 1038, 1060, 1177
Hin6I GCGC 1 cut(s) 707
HinP1I GCGC 1 cut(s) 707
HinfI GANTC 7 cut(s) 131, 470, 570, 590, 733, 977, 1062
HpaII CCGG 7 cut(s) 158, 422, 437, 816, 1119, 1244, 1290
HphI GGTGA 5 cut(s) 140, 460, 625, 1351, 1400
Hpy166II GTNNAC 2 cut(s) 529, 1330
Hpy188I TCNGA 3 cut(s) 79, 595, 1091
Hpy8I GTNNAC 2 cut(s) 529, 1330
Hpy99I CGWCG 1 cut(s) 1437
HpyAV CCTTC 8 cut(s) 104, 396, 487, 500, 739, 1129, 1138, 1439
HpyCH4III ACNGT 4 cut(s) 637, 886, 1159, 1185
HpyCH4IV ACGT 1 cut(s) 1435
HpyF3I CTNAG 5 cut(s) 43, 480, 554, 837, 957
HpySE526I ACGT 1 cut(s) 1435
Hsp92I GRCGYC 1 cut(s) 1435
Hsp92II CATG 9 cut(s) 112, 775, 779, 829, 859, 913, 1038, 1060, 1177
HspAI GCGC 1 cut(s) 707
LmnI GCTCC 2 cut(s) 105, 373
Lsp1109I GCAGC 6 cut(s) 22, 331, 352, 696, 710, 736
LweI GCATC 4 cut(s) 306, 633, 694, 1386
MaeI CTAG 5 cut(s) 252, 804, 953, 1310, 1441
MaeII ACGT 1 cut(s) 1435
MaeIII GTNAC 4 cut(s) 631, 786, 1121, 1436
MboII GAAGA 9 cut(s) 196, 410, 565, 754, 898, 994, 1022, 1354, 1390
MflI RGATCY 1 cut(s) 416
MhlI GDGCHC 2 cut(s) 963, 1289
MlsI TGGCCA 2 cut(s) 539, 890
MluCI AATT 4 cut(s) 163, 263, 600, 619
MluNI TGGCCA 2 cut(s) 539, 890
MlyI GAGTC 2 cut(s) 464, 971
MmeI TCCRAC 1 cut(s) 901
Mox20I TGGCCA 2 cut(s) 539, 890
Mph1103I ATGCAT 3 cut(s) 648, 777, 1240
MroXI GAANNNNTTC 1 cut(s) 1346
MscI TGGCCA 2 cut(s) 539, 890
MslI CAYNNNNRTG 1 cut(s) 896
Msp20I TGGCCA 2 cut(s) 539, 890
MspA1I CMGCKG 1 cut(s) 374
MspI CCGG 7 cut(s) 158, 422, 437, 816, 1119, 1244, 1290
MspR9I CCNGG 7 cut(s) 102, 423, 669, 990, 1244, 1290, 1291
Mva1269I GAATGC 3 cut(s) 646, 1238, 1247
MvaI CCWGG 3 cut(s) 102, 669, 990
MvnI CGCG 1 cut(s) 393
NciI CCSGG 4 cut(s) 423, 1244, 1290, 1291
NdeI CATATG 1 cut(s) 792
NlaIII CATG 9 cut(s) 112, 775, 779, 829, 859, 913, 1038, 1060, 1177
NlaIV GGNNCC 3 cut(s) 769, 1260, 1276
NmuCI GTSAC 2 cut(s) 786, 1436
NruI TCGCGA 1 cut(s) 393
NsiI ATGCAT 3 cut(s) 648, 777, 1240
NspI RCATGY 1 cut(s) 829
NspV TTCGAA 1 cut(s) 1350
PagI TCATGA 2 cut(s) 909, 1056
PceI AGGCCT 2 cut(s) 497, 1097
PcsI WCGNNNNNNNCGW 2 cut(s) 591, 1432
PctI GAATGC 3 cut(s) 646, 1238, 1247
PdmI GAANNNNTTC 1 cut(s) 1346
PfeI GAWTC 5 cut(s) 131, 570, 590, 733, 1062
PfoI TCCNGGA 1 cut(s) 667
PkrI GCNGC 8 cut(s) 37, 346, 349, 367, 700, 711, 751, 760
PleI GAGTC 2 cut(s) 464, 971
PpsI GAGTC 2 cut(s) 464, 971
PpuMI RGGWCCY 4 cut(s) 767, 949, 1228, 1274
PsiI TTATAA 1 cut(s) 413
Psp5II RGGWCCY 4 cut(s) 767, 949, 1228, 1274
Psp6I CCWGG 3 cut(s) 100, 667, 988
PspGI CCWGG 3 cut(s) 100, 667, 988
PspN4I GGNNCC 3 cut(s) 769, 1260, 1276
PspPI GGNCC 5 cut(s) 248, 767, 949, 1228, 1274
PspPPI RGGWCCY 4 cut(s) 767, 949, 1228, 1274
PsrI GAACNNNNNNTAC 2 cut(s) 1169, 1201
PstI CTGCAG 2 cut(s) 569, 874
PsuI RGATCY 1 cut(s) 416
RruI TCGCGA 1 cut(s) 393
RsaI GTAC 7 cut(s) 191, 290, 455, 1031, 1165, 1187, 1301
RsaNI GTAC 7 cut(s) 190, 289, 454, 1030, 1164, 1186, 1300
RseI CAYNNNNRTG 1 cut(s) 896
SatI GCNGC 8 cut(s) 36, 345, 348, 366, 699, 710, 750, 759
Sau96I GGNCC 5 cut(s) 248, 767, 949, 1228, 1274
ScaI AGTACT 4 cut(s) 191, 290, 1031, 1187
SchI GAGTC 2 cut(s) 464, 971
ScrFI CCNGG 7 cut(s) 102, 423, 669, 990, 1244, 1290, 1291
SduI GDGCHC 2 cut(s) 963, 1289
SfaNI GCATC 4 cut(s) 306, 633, 694, 1386
SfcI CTRYAG 2 cut(s) 565, 870
SfuI TTCGAA 1 cut(s) 1350
SinI GGWCC 5 cut(s) 248, 767, 949, 1228, 1274
SmaI CCCGGG 1 cut(s) 1291
SmiMI CAYNNNNRTG 1 cut(s) 896
Sse9I AATT 4 cut(s) 163, 263, 600, 619
SseBI AGGCCT 2 cut(s) 497, 1097
SsiI CCGC 5 cut(s) 255, 348, 372, 759, 1070
SspMI CTAG 5 cut(s) 252, 804, 953, 1310, 1441
StuI AGGCCT 2 cut(s) 497, 1097
StyD4I CCNGG 7 cut(s) 100, 421, 667, 988, 1242, 1288, 1289
StyI CCWWGG 2 cut(s) 945, 1371
TaaI ACNGT 4 cut(s) 637, 886, 1159, 1185
TaiI ACGT 1 cut(s) 1438
TaqI TCGA 8 cut(s) 138, 304, 314, 331, 585, 980, 1350, 1432
TasI AATT 4 cut(s) 163, 263, 600, 619
TatI WGTACW 5 cut(s) 189, 288, 1029, 1163, 1185
TauI GCSGC 2 cut(s) 350, 761
TfiI GAWTC 5 cut(s) 131, 570, 590, 733, 1062
TscAI CASTG 4 cut(s) 361, 889, 898, 1260
TseFI GTSAC 2 cut(s) 786, 1436
TseI GCWGC 6 cut(s) 35, 344, 365, 698, 709, 749
Tsp45I GTSAC 2 cut(s) 786, 1436
TspDTI ATGAA 8 cut(s) 294, 411, 506, 898, 926, 1040, 1065, 1190
TspMI CCCGGG 1 cut(s) 1289
TspRI CASTG 4 cut(s) 361, 889, 898, 1260
VpaK11BI GGWCC 5 cut(s) 248, 767, 949, 1228, 1274
XapI RAATTY 2 cut(s) 163, 263
XceI RCATGY 1 cut(s) 829
XmaI CCCGGG 1 cut(s) 1289
XmiI GTMKAC 1 cut(s) 528
XmnI GAANNNNTTC 1 cut(s) 1346
XspI CTAG 5 cut(s) 252, 804, 953, 1310, 1441
ZraI GACGTC 1 cut(s) 1436
ZrmI AGTACT 4 cut(s) 191, 290, 1031, 1187
Zsp2I ATGCAT 3 cut(s) 648, 777, 1240
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.