pycom10g29100

Ras-related protein

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
pyrus_communis
Chr10
OG0005599
Physical Location & Seq
Reverse (-)
29502119 .. 29503273
1155 bp
Loading structure...
UTR
Exon/CDS
Intron
pycom10g29100.1

Sequence Viewer

Length: 303 bp
ATGACAATTATGCTCATTGGTAATAAGTGTGATCTAGCTCACAGACGGGCTGTGAGCACGGAGGAAGGGGAGCAATTTGCAAAGGAGCATGGCTTGATCTTCATGGAGGCCTCTGCTAAAACTGCTCAGAATGTTGAGGAGGCATTTATAAAAACTGCTGCAACCATATACAAAAAGATTCAAGATGGAGTGTTTGATGTATCAAATGAGTCTTATGGCATAAAAGTTGGATATGGTGGAATTCCGGGACCATCAGGAGGTAGAGATGGCGCTTCATCTCAAGCTGGAGGCTGTTGTAGTTGA

Protein Analysis

101

Amino Acids

10.5

Weight (kDa)

5.61

Isoelectric Point (pI)

29.95

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
Ras PF00071 2 - 57 1.6e-17 Ras family
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AanI TTATAA 1 cut(s) 149
AcsI RAATTY 1 cut(s) 240
AfiI CCNNNNNNNGG 1 cut(s) 257
AgsI TTSAA 1 cut(s) 182
AluBI AGCT 2 cut(s) 38, 284
AluI AGCT 2 cut(s) 38, 284
Alw21I GWGCWC 1 cut(s) 59
AoxI GGCC 1 cut(s) 108
ApeKI GCWGC 1 cut(s) 158
ApoI RAATTY 1 cut(s) 240
AspLEI GCGC 1 cut(s) 272
AspS9I GGNCC 1 cut(s) 248
AsuC2I CCSGG 1 cut(s) 246
AvaII GGWCC 1 cut(s) 248
Bbv12I GWGCWC 1 cut(s) 59
BbvI GCAGC 1 cut(s) 145
BccI CCATC 3 cut(s) 179, 259, 260
BcnI CCSGG 1 cut(s) 246
BfaI CTAG 1 cut(s) 35
BfoI RGCGCY 1 cut(s) 273
BisI GCNGC 1 cut(s) 159
BlsI GCNGC 1 cut(s) 160
Bme1390I CCNGG 1 cut(s) 246
Bme18I GGWCC 1 cut(s) 248
BmgT120I GGNCC 1 cut(s) 248
BmiI GGNNCC 1 cut(s) 249
BmrFI CCNGG 1 cut(s) 246
BpuEI CTTGAG 1 cut(s) 264
BpuMI CCSGG 1 cut(s) 246
Bsc4I CCNNNNNNNGG 1 cut(s) 257
BseLI CCNNNNNNNGG 1 cut(s) 257
BseMII CTCAG 1 cut(s) 140
BseRI GAGGAG 1 cut(s) 152
BseXI GCAGC 1 cut(s) 145
BshFI GGCC 1 cut(s) 110
BsiHKAI GWGCWC 1 cut(s) 59
BsiSI CCGG 1 cut(s) 245
BslFI GGGAC 1 cut(s) 261
BslI CCNNNNNNNGG 1 cut(s) 257
BsmFI GGGAC 1 cut(s) 261
BsnI GGCC 1 cut(s) 110
Bsp1286I GDGCHC 1 cut(s) 59
Bsp143I GATC 2 cut(s) 31, 96
BspANI GGCC 1 cut(s) 110
BspCNI CTCAG 1 cut(s) 139
BspLI GGNNCC 1 cut(s) 249
BssMI GATC 2 cut(s) 31, 96
BstDEI CTNAG 1 cut(s) 126
BstH2I RGCGCY 1 cut(s) 273
BstHHI GCGC 1 cut(s) 272
BstKTI GATC 2 cut(s) 34, 99
BstMBI GATC 2 cut(s) 31, 96
BstMWI GCNNNNNNNGC 1 cut(s) 122
BstSCI CCNGG 1 cut(s) 244
BstV1I GCAGC 1 cut(s) 145
BsuRI GGCC 1 cut(s) 110
CfoI GCGC 1 cut(s) 272
Cfr13I GGNCC 1 cut(s) 248
CviAII CATG 2 cut(s) 89, 103
CviJI RGCY 6 cut(s) 38, 50, 93, 110, 284, 291
CviKI_1 RGCY 6 cut(s) 38, 50, 93, 110, 284, 291
DdeI CTNAG 1 cut(s) 126
DpnI GATC 2 cut(s) 33, 98
DpnII GATC 2 cut(s) 31, 96
Eco147I AGGCCT 1 cut(s) 110
Eco47I GGWCC 1 cut(s) 248
EcoRI GAATTC 1 cut(s) 240
FaeI CATG 2 cut(s) 92, 106
FaiI YATR 9 cut(s) 11, 90, 104, 149, 167, 169, 216, 221, 234
FaqI GGGAC 1 cut(s) 261
FatI CATG 2 cut(s) 88, 102
Fnu4HI GCNGC 1 cut(s) 159
Fsp4HI GCNGC 1 cut(s) 159
FspBI CTAG 1 cut(s) 35
GlaI GCGC 1 cut(s) 271
GluI GCNGC 1 cut(s) 159
HaeII RGCGCY 1 cut(s) 273
HaeIII GGCC 1 cut(s) 110
HapII CCGG 1 cut(s) 245
HhaI GCGC 1 cut(s) 272
Hin1II CATG 2 cut(s) 92, 106
Hin6I GCGC 1 cut(s) 270
HinP1I GCGC 1 cut(s) 270
HinfI GANTC 2 cut(s) 178, 209
HpaII CCGG 1 cut(s) 245
Hpy188I TCNGA 1 cut(s) 129
Hpy188III TCNNGA 2 cut(s) 182, 255
HpyAV CCTTC 1 cut(s) 59
HpyCH4V TGCA 2 cut(s) 80, 161
HpyF10VI GCNNNNNNNGC 1 cut(s) 122
HpyF3I CTNAG 1 cut(s) 126
Hsp92II CATG 2 cut(s) 92, 106
HspAI GCGC 1 cut(s) 270
Kzo9I GATC 2 cut(s) 31, 96
LmnI GCTCC 2 cut(s) 70, 85
LpnPI CCDG 3 cut(s) 240, 258, 270
Lsp1109I GCAGC 1 cut(s) 145
MaeI CTAG 1 cut(s) 35
MalI GATC 2 cut(s) 33, 98
MboI GATC 2 cut(s) 31, 96
MboII GAAGA 1 cut(s) 91
MhlI GDGCHC 1 cut(s) 59
MluCI AATT 3 cut(s) 6, 74, 240
MlyI GAGTC 1 cut(s) 218
MmeI TCCRAC 1 cut(s) 208
MnlI CCTC 7 cut(s) 55, 100, 121, 130, 133, 251, 281
MspI CCGG 1 cut(s) 245
MspR9I CCNGG 1 cut(s) 246
MwoI GCNNNNNNNGC 1 cut(s) 122
NciI CCSGG 1 cut(s) 246
NdeII GATC 2 cut(s) 31, 96
NlaIII CATG 2 cut(s) 92, 106
NlaIV GGNNCC 1 cut(s) 249
PceI AGGCCT 1 cut(s) 110
PfeI GAWTC 1 cut(s) 178
PfoI TCCNGGA 1 cut(s) 244
PkrI GCNGC 1 cut(s) 160
PleI GAGTC 1 cut(s) 217
PpsI GAGTC 1 cut(s) 217
PsiI TTATAA 1 cut(s) 149
PspN4I GGNNCC 1 cut(s) 249
PspPI GGNCC 1 cut(s) 248
SatI GCNGC 1 cut(s) 159
Sau3AI GATC 2 cut(s) 31, 96
Sau96I GGNCC 1 cut(s) 248
SchI GAGTC 1 cut(s) 218
ScrFI CCNGG 1 cut(s) 246
SduI GDGCHC 1 cut(s) 59
SetI ASST 3 cut(s) 40, 262, 286
SinI GGWCC 1 cut(s) 248
SmlI CTYRAG 1 cut(s) 279
SmoI CTYRAG 1 cut(s) 279
Sse9I AATT 3 cut(s) 6, 74, 240
SseBI AGGCCT 1 cut(s) 110
SspMI CTAG 1 cut(s) 35
StuI AGGCCT 1 cut(s) 110
StyD4I CCNGG 1 cut(s) 244
TasI AATT 3 cut(s) 6, 74, 240
TfiI GAWTC 1 cut(s) 178
TseI GCWGC 1 cut(s) 158
TspDTI ATGAA 2 cut(s) 91, 264
TspGWI ACGGA 1 cut(s) 74
VpaK11BI GGWCC 1 cut(s) 248
XapI RAATTY 1 cut(s) 240
XspI CTAG 1 cut(s) 35
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.