Rw1G021010

Ras-related protein

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
rosa_wichuraiana
Chr1
OG0005599
Physical Location & Seq
Forward (+)
44858920 .. 44861116
2197 bp
Loading structure...
UTR
Exon/CDS
Intron
Rw1G021010.1

Sequence Viewer

Length: 414 bp
ATGACCATAATGTTAATTGGTAATAAGTGTGATCTTGCTCATAGACGACGAGCTGTGAGCACAGAGGAAGGGGAGCAATTTGCAGAGGAACATGGGTTGATCTTCATGGAGGCCTCTGCTAAAACTGGTCAGAATGTTGAGGAGGGATTCATAAAAACTGCTGCAACCATATACAAGAAGATTCAAGATGGCGTTTTTGATGTATCAAATGAGTTCTCTGCCCGACCGAAGATTGGTACATATGGCCGTTACCTCCGTTACGGAACTTGCCAGCTGACAAATTTACAAGGGTACTTTCGTCCATTCACATATCAATTAATTTTTTTTTTGAAAAATGGTCAGTACGGTACCCTGTCTTTGGCTCCTTATAACTTTTGGTACCTGACAAAAAAAAAATATCAATACGGTACCTGA

Protein Analysis

137

Amino Acids

15.8

Weight (kDa)

9.18

Isoelectric Point (pI)

24.51

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
Ras PF00071 2 - 58 5.5e-15 Ras family
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AanI TTATAA 1 cut(s) 369
Acc65I GGTACC 3 cut(s) 347, 378, 407
AccB1I GGYRCC 3 cut(s) 347, 378, 407
AcoI YGGCCR 1 cut(s) 244
AcsI RAATTY 1 cut(s) 280
AfaI GTAC 6 cut(s) 238, 293, 344, 349, 380, 409
AfiI CCNNNNNNNGG 2 cut(s) 233, 358
AgsI TTSAA 2 cut(s) 185, 331
AluBI AGCT 2 cut(s) 53, 274
AluI AGCT 2 cut(s) 53, 274
Alw21I GWGCWC 1 cut(s) 62
AoxI GGCC 2 cut(s) 111, 244
ApeKI GCWGC 1 cut(s) 161
ApoI RAATTY 1 cut(s) 280
AseI ATTAAT 1 cut(s) 317
Asp718I GGTACC 3 cut(s) 347, 378, 407
BaeI ACNNNNGTAYC 2 cut(s) 283, 316
BanI GGYRCC 3 cut(s) 347, 378, 407
Bbv12I GWGCWC 1 cut(s) 62
BbvI GCAGC 1 cut(s) 148
BccI CCATC 1 cut(s) 182
BceAI ACGGC 1 cut(s) 231
BisI GCNGC 1 cut(s) 162
BlsI GCNGC 1 cut(s) 163
BmiI GGNNCC 4 cut(s) 349, 363, 380, 409
Bsc4I CCNNNNNNNGG 2 cut(s) 233, 358
Bse1I ACTGG 1 cut(s) 130
BseLI CCNNNNNNNGG 2 cut(s) 233, 358
BseNI ACTGG 1 cut(s) 130
BseRI GAGGAG 1 cut(s) 155
BseXI GCAGC 1 cut(s) 148
Bsh1285I CGRYCG 1 cut(s) 227
BshFI GGCC 2 cut(s) 113, 246
BshNI GGYRCC 3 cut(s) 347, 378, 407
BsiEI CGRYCG 1 cut(s) 227
BsiHKAI GWGCWC 1 cut(s) 62
BslI CCNNNNNNNGG 2 cut(s) 233, 358
BsnI GGCC 2 cut(s) 113, 246
Bsp1286I GDGCHC 1 cut(s) 62
Bsp143I GATC 2 cut(s) 31, 99
BspANI GGCC 2 cut(s) 113, 246
BspLI GGNNCC 4 cut(s) 349, 363, 380, 409
BspT107I GGYRCC 3 cut(s) 347, 378, 407
BsrI ACTGG 1 cut(s) 130
BssMI GATC 2 cut(s) 31, 99
Bst4CI ACNGT 2 cut(s) 347, 407
BstC8I GCNNGC 1 cut(s) 272
BstKTI GATC 2 cut(s) 34, 102
BstMBI GATC 2 cut(s) 31, 99
BstMCI CGRYCG 1 cut(s) 227
BstV1I GCAGC 1 cut(s) 148
BsuRI GGCC 2 cut(s) 113, 246
Cac8I GCNNGC 1 cut(s) 272
Csp6I GTAC 6 cut(s) 237, 292, 343, 348, 379, 408
CviAII CATG 2 cut(s) 92, 106
CviJI RGCY 5 cut(s) 53, 113, 246, 274, 362
CviKI_1 RGCY 5 cut(s) 53, 113, 246, 274, 362
CviQI GTAC 6 cut(s) 237, 292, 343, 348, 379, 408
DpnI GATC 2 cut(s) 33, 101
DpnII GATC 2 cut(s) 31, 99
EaeI YGGCCR 1 cut(s) 244
Eco147I AGGCCT 1 cut(s) 113
FaeI CATG 2 cut(s) 95, 109
FatI CATG 2 cut(s) 91, 105
FauNDI CATATG 1 cut(s) 241
Fnu4HI GCNGC 1 cut(s) 162
Fsp4HI GCNGC 1 cut(s) 162
GluI GCNGC 1 cut(s) 162
HaeIII GGCC 2 cut(s) 113, 246
Hin1II CATG 2 cut(s) 95, 109
HinfI GANTC 2 cut(s) 147, 181
Hpy188I TCNGA 1 cut(s) 132
Hpy188III TCNNGA 1 cut(s) 185
Hpy99I CGWCG 1 cut(s) 51
HpyAV CCTTC 1 cut(s) 62
HpyCH4III ACNGT 2 cut(s) 347, 407
HpyCH4V TGCA 2 cut(s) 83, 164
Hsp92II CATG 2 cut(s) 95, 109
KpnI GGTACC 3 cut(s) 351, 382, 411
Kzo9I GATC 2 cut(s) 31, 99
LmnI GCTCC 2 cut(s) 73, 367
LpnPI CCDG 4 cut(s) 111, 284, 365, 395
Lsp1109I GCAGC 1 cut(s) 148
MaeIII GTNAC 2 cut(s) 248, 257
MalI GATC 2 cut(s) 33, 101
MboI GATC 2 cut(s) 31, 99
MboII GAAGA 3 cut(s) 94, 190, 241
MhlI GDGCHC 1 cut(s) 62
MluCI AATT 5 cut(s) 15, 77, 280, 314, 318
MnlI CCTC 7 cut(s) 58, 79, 103, 124, 133, 136, 263
MseI TTAA 2 cut(s) 14, 317
MspA1I CMGCKG 1 cut(s) 274
NdeI CATATG 1 cut(s) 241
NdeII GATC 2 cut(s) 31, 99
NlaIII CATG 2 cut(s) 95, 109
NlaIV GGNNCC 4 cut(s) 349, 363, 380, 409
PceI AGGCCT 1 cut(s) 113
PfeI GAWTC 2 cut(s) 147, 181
PkrI GCNGC 1 cut(s) 163
PshBI ATTAAT 1 cut(s) 317
PsiI TTATAA 1 cut(s) 369
PspN4I GGNNCC 4 cut(s) 349, 363, 380, 409
PvuII CAGCTG 1 cut(s) 274
RsaI GTAC 6 cut(s) 238, 293, 344, 349, 380, 409
RsaNI GTAC 6 cut(s) 237, 292, 343, 348, 379, 408
SaqAI TTAA 2 cut(s) 14, 317
SatI GCNGC 1 cut(s) 162
Sau3AI GATC 2 cut(s) 31, 99
SduI GDGCHC 1 cut(s) 62
SetI ASST 5 cut(s) 55, 255, 276, 384, 413
Sse9I AATT 5 cut(s) 15, 77, 280, 314, 318
SseBI AGGCCT 1 cut(s) 113
StuI AGGCCT 1 cut(s) 113
TaaI ACNGT 2 cut(s) 347, 407
TaqII GACCGA 1 cut(s) 241
TasI AATT 5 cut(s) 15, 77, 280, 314, 318
TfiI GAWTC 2 cut(s) 147, 181
Tru1I TTAA 2 cut(s) 14, 317
Tru9I TTAA 2 cut(s) 14, 317
TseI GCWGC 1 cut(s) 161
TspDTI ATGAA 2 cut(s) 94, 139
TspGWI ACGGA 2 cut(s) 245, 276
VspI ATTAAT 1 cut(s) 317
XapI RAATTY 1 cut(s) 280
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.