pycom11g02120

Belongs to the glycosyl hydrolase 1 family

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
pyrus_communis
Chr11
OG0010589
Physical Location & Seq
Forward (+)
1812762 .. 1813343
582 bp
Loading structure...
UTR
Exon/CDS
Intron
N/A

Sequence Viewer

Length: 315 bp
ATGGCAATACAGTTGCGATGTTGGATCGTATGCTTGTTACAATTAGTGTTACTAGTTAGCGTTGCACTGACAAATGGCACAGATGCTAGTACGGATCCGCCCATTCATTGTGGCTCTCTCAGCAGACGTAGTTTTGAACCAGGGTTCATATTTGGCACAGCTTCGGCATCCTATCAGTTCGAAGGTGCTGCAAAAGAAGATGGTAGAAGACCGAGCATATGGGATACCTACACGCACGCCCATCCAGAAAGAATTGTAGATTGCAGCAACAGAGATGTTGCAGTTGATCAATATCACCGCTATAAGGTTAAATAA

Protein Analysis

105

Amino Acids

11.66

Weight (kDa)

6.88

Isoelectric Point (pI)

34.12

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
Glyco_hydro_1 PF00232 43 - 102 1e-19 Glycosyl hydrolase family 1
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

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Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AciI CCGC 2 cut(s) 98, 298
AclWI GGATC 3 cut(s) 32, 89, 102
AfaI GTAC 1 cut(s) 91
AfiI CCNNNNNNNGG 1 cut(s) 304
AgsI TTSAA 1 cut(s) 137
AhlI ACTAGT 1 cut(s) 52
AjnI CCWGG 1 cut(s) 139
AluBI AGCT 1 cut(s) 161
AluI AGCT 1 cut(s) 161
AlwI GGATC 3 cut(s) 32, 89, 102
ApeKI GCWGC 2 cut(s) 188, 264
AsuHPI GGTGA 1 cut(s) 287
AsuII TTCGAA 1 cut(s) 180
BamHI GGATCC 1 cut(s) 94
BbsI GAAGAC 1 cut(s) 214
BbvI GCAGC 2 cut(s) 175, 276
BccI CCATC 2 cut(s) 194, 249
BcgI CGANNNNNNTGC 2 cut(s) 170, 204
BciT130I CCWGG 1 cut(s) 141
BciVI GTATCC 1 cut(s) 217
BclI TGATCA 1 cut(s) 286
BcuI ACTAGT 1 cut(s) 52
BfaI CTAG 2 cut(s) 53, 87
BfuI GTATCC 1 cut(s) 217
BisI GCNGC 2 cut(s) 189, 265
BlsI GCNGC 2 cut(s) 190, 266
Bme1390I CCNGG 1 cut(s) 141
BmiI GGNNCC 1 cut(s) 96
BmrFI CCNGG 1 cut(s) 141
BmsI GCATC 2 cut(s) 73, 176
BpiI GAAGAC 1 cut(s) 214
Bpu14I TTCGAA 1 cut(s) 180
BsaBI GATNNNNATC 1 cut(s) 291
BsaJI CCNNGG 1 cut(s) 140
Bsc4I CCNNNNNNNGG 1 cut(s) 304
Bse8I GATNNNNATC 1 cut(s) 291
BseBI CCWGG 1 cut(s) 141
BseDI CCNNGG 1 cut(s) 140
BseGI GGATG 2 cut(s) 167, 241
BseJI GATNNNNATC 1 cut(s) 291
BseLI CCNNNNNNNGG 1 cut(s) 304
BseMII CTCAG 1 cut(s) 133
BseXI GCAGC 2 cut(s) 175, 276
BslI CCNNNNNNNGG 1 cut(s) 304
Bsp119I TTCGAA 1 cut(s) 180
Bsp143I GATC 3 cut(s) 24, 94, 286
BspACI CCGC 2 cut(s) 98, 298
BspCNI CTCAG 1 cut(s) 132
BspLI GGNNCC 1 cut(s) 96
BspPI GGATC 3 cut(s) 32, 89, 102
BspT104I TTCGAA 1 cut(s) 180
BssECI CCNNGG 1 cut(s) 140
BssMI GATC 3 cut(s) 24, 94, 286
Bst2UI CCWGG 1 cut(s) 141
Bst4CI ACNGT 1 cut(s) 12
BstBI TTCGAA 1 cut(s) 180
BstC8I GCNNGC 1 cut(s) 237
BstDEI CTNAG 1 cut(s) 119
BstF5I GGATG 2 cut(s) 167, 241
BstKTI GATC 3 cut(s) 27, 97, 289
BstMBI GATC 3 cut(s) 24, 94, 286
BstMWI GCNNNNNNNGC 1 cut(s) 120
BstNI CCWGG 1 cut(s) 141
BstSCI CCNGG 1 cut(s) 139
BstV1I GCAGC 2 cut(s) 175, 276
BstV2I GAAGAC 1 cut(s) 214
BstX2I RGATCY 1 cut(s) 94
BstYI RGATCY 1 cut(s) 94
BsuI GTATCC 1 cut(s) 217
BtgZI GCGATG 1 cut(s) 31
BtsCI GGATG 2 cut(s) 167, 241
BtsIMutI CAGTG 1 cut(s) 65
Cac8I GCNNGC 1 cut(s) 237
Csp6I GTAC 1 cut(s) 90
CviJI RGCY 2 cut(s) 114, 161
CviKI_1 RGCY 2 cut(s) 114, 161
CviQI GTAC 1 cut(s) 90
DdeI CTNAG 1 cut(s) 119
DpnI GATC 3 cut(s) 26, 96, 288
DpnII GATC 3 cut(s) 24, 94, 286
EciI GGCGGA 1 cut(s) 87
EcoRII CCWGG 1 cut(s) 139
FaiI YATR 5 cut(s) 31, 149, 218, 220, 303
FauNDI CATATG 1 cut(s) 218
FbaI TGATCA 1 cut(s) 286
Fnu4HI GCNGC 2 cut(s) 189, 265
FokI GGATG 2 cut(s) 154, 228
Fsp4HI GCNGC 2 cut(s) 189, 265
FspBI CTAG 2 cut(s) 53, 87
GluI GCNGC 2 cut(s) 189, 265
HphI GGTGA 1 cut(s) 287
Hpy188III TCNNGA 1 cut(s) 245
HpyAV CCTTC 1 cut(s) 176
HpyCH4III ACNGT 1 cut(s) 12
HpyCH4IV ACGT 1 cut(s) 127
HpyCH4V TGCA 4 cut(s) 65, 191, 264, 281
HpyF10VI GCNNNNNNNGC 1 cut(s) 120
HpyF3I CTNAG 1 cut(s) 119
HpySE526I ACGT 1 cut(s) 127
Ksp22I TGATCA 1 cut(s) 286
Kzo9I GATC 3 cut(s) 24, 94, 286
LpnPI CCDG 3 cut(s) 126, 153, 258
Lsp1109I GCAGC 2 cut(s) 175, 276
LweI GCATC 2 cut(s) 73, 176
MaeI CTAG 2 cut(s) 53, 87
MaeII ACGT 1 cut(s) 127
MaeIII GTNAC 2 cut(s) 36, 48
MalI GATC 3 cut(s) 26, 96, 288
MboI GATC 3 cut(s) 24, 94, 286
MboII GAAGA 2 cut(s) 209, 219
MflI RGATCY 1 cut(s) 94
MluCI AATT 2 cut(s) 41, 252
MseI TTAA 1 cut(s) 309
MspR9I CCNGG 1 cut(s) 141
MvaI CCWGG 1 cut(s) 141
MwoI GCNNNNNNNGC 1 cut(s) 120
NdeI CATATG 1 cut(s) 218
NdeII GATC 3 cut(s) 24, 94, 286
NlaIV GGNNCC 1 cut(s) 96
NspV TTCGAA 1 cut(s) 180
PkrI GCNGC 2 cut(s) 190, 266
Psp6I CCWGG 1 cut(s) 139
PspGI CCWGG 1 cut(s) 139
PspN4I GGNNCC 1 cut(s) 96
PsuI RGATCY 1 cut(s) 94
RsaI GTAC 1 cut(s) 91
RsaNI GTAC 1 cut(s) 90
SaqAI TTAA 1 cut(s) 309
SatI GCNGC 2 cut(s) 189, 265
Sau3AI GATC 3 cut(s) 24, 94, 286
ScrFI CCNGG 1 cut(s) 141
SetI ASST 5 cut(s) 130, 163, 187, 230, 309
SfaNI GCATC 2 cut(s) 73, 176
SfuI TTCGAA 1 cut(s) 180
SgeI CNNG 9 cut(s) 46, 65, 99, 152, 153, 225, 244, 248, 257
SpeI ACTAGT 1 cut(s) 52
Sse9I AATT 2 cut(s) 41, 252
SsiI CCGC 2 cut(s) 98, 298
SspMI CTAG 2 cut(s) 53, 87
StyD4I CCNGG 1 cut(s) 139
TaaI ACNGT 1 cut(s) 12
TaiI ACGT 1 cut(s) 130
TaqI TCGA 1 cut(s) 180
TaqII GACCGA 1 cut(s) 226
TasI AATT 2 cut(s) 41, 252
Tru1I TTAA 1 cut(s) 309
Tru9I TTAA 1 cut(s) 309
TscAI CASTG 1 cut(s) 72
TseI GCWGC 2 cut(s) 188, 264
TspDTI ATGAA 2 cut(s) 95, 136
TspGWI ACGGA 1 cut(s) 107
TspRI CASTG 1 cut(s) 72
XspI CTAG 2 cut(s) 53, 87
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.