Rh5BG534300

Belongs to the glycosyl hydrolase 1 family

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
rosa_samantha
Chr5B
OG0010589
Physical Location & Seq
Forward (+)
84250324 .. 84255957
5634 bp
Loading structure...
UTR
Exon/CDS
Intron
Rh5BG534300.1

Sequence Viewer

Length: 357 bp
ATGGCAATGCAAGGATCTCTGTTTCTAGGCGTAGTGCTTATACATGTTGCAGGCTTTAAATTGACAAGCAGTGAAGCTCTTACACCCAGTACTCCTATCTATGACACTGGTTTCCTCAACAGGACTAGTTTCCCAGCAGGTTTCATATTTGGGACAGCTTCAGCGTCATATCAGTATGAAGGTGCTGCTAAAGAAGGTGGTAGAGGACCCAGCATATGGGATACCTACACCCACGAACATCCAGGTGCCTATACTCTCTTTCCCCTTATGTGGGGAGACGTGTTCACACCAATGGAACATAGCCAAGCGACAAATCTTCCGATCTCCATTGCGCCCGTCCAGTCTTCACAAAGGTAG

Protein Analysis

118

Amino Acids

12.73

Weight (kDa)

5.67

Isoelectric Point (pI)

24.19

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
Glyco_hydro_1 PF00232 42 - 80 3.8e-13 Glycosyl hydrolase family 1
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

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Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
Acc36I ACCTGC 1 cut(s) 128
AccB1I GGYRCC 1 cut(s) 245
AccB7I CCANNNNNTGG 1 cut(s) 216
AclWI GGATC 1 cut(s) 22
AcuI CTGAAG 1 cut(s) 144
AfaI GTAC 1 cut(s) 91
AfiI CCNNNNNNNGG 3 cut(s) 216, 270, 271
AflIII ACRYGT 2 cut(s) 43, 279
AhlI ACTAGT 1 cut(s) 125
AjiI CACGTC 1 cut(s) 280
AjnI CCWGG 1 cut(s) 241
AluBI AGCT 2 cut(s) 77, 158
AluI AGCT 2 cut(s) 77, 158
Alw26I GTCTC 1 cut(s) 270
AlwI GGATC 1 cut(s) 22
ApeKI GCWGC 1 cut(s) 185
AspLEI GCGC 1 cut(s) 334
AspS9I GGNCC 1 cut(s) 206
AvaII GGWCC 1 cut(s) 206
BanI GGYRCC 1 cut(s) 245
BbsI GAAGAC 1 cut(s) 336
BbvI GCAGC 1 cut(s) 172
BciT130I CCWGG 1 cut(s) 243
BciVI GTATCC 1 cut(s) 214
BcoDI GTCTC 1 cut(s) 270
BcuI ACTAGT 1 cut(s) 125
BfaI CTAG 2 cut(s) 26, 126
BfuAI ACCTGC 1 cut(s) 128
BfuI GTATCC 1 cut(s) 214
BisI GCNGC 1 cut(s) 186
BlsI GCNGC 1 cut(s) 187
BmcAI AGTACT 1 cut(s) 91
Bme1390I CCNGG 1 cut(s) 243
Bme18I GGWCC 1 cut(s) 206
BmgBI CACGTC 1 cut(s) 280
BmgT120I GGNCC 1 cut(s) 206
BmiI GGNNCC 2 cut(s) 208, 247
BmrFI CCNGG 1 cut(s) 243
BmrI ACTGGG 1 cut(s) 81
BmuI ACTGGG 1 cut(s) 81
BpiI GAAGAC 1 cut(s) 336
Bsc4I CCNNNNNNNGG 3 cut(s) 216, 270, 271
Bse1I ACTGG 3 cut(s) 87, 112, 340
Bse3DI GCAATG 2 cut(s) 12, 327
BseBI CCWGG 1 cut(s) 243
BseGI GGATG 1 cut(s) 238
BseLI CCNNNNNNNGG 3 cut(s) 216, 270, 271
BseMI GCAATG 2 cut(s) 12, 327
BseNI ACTGG 3 cut(s) 87, 112, 340
BseXI GCAGC 1 cut(s) 172
BseYI CCCAGC 2 cut(s) 133, 209
BshNI GGYRCC 1 cut(s) 245
BslFI GGGAC 1 cut(s) 166
BslI CCNNNNNNNGG 3 cut(s) 216, 270, 271
BsmAI GTCTC 1 cut(s) 270
BsmBI CGTCTC 1 cut(s) 270
BsmFI GGGAC 1 cut(s) 166
Bsp143I GATC 2 cut(s) 14, 321
BspLI GGNNCC 2 cut(s) 208, 247
BspMI ACCTGC 1 cut(s) 128
BspPI GGATC 1 cut(s) 22
BspT107I GGYRCC 1 cut(s) 245
BsrDI GCAATG 2 cut(s) 12, 327
BsrI ACTGG 3 cut(s) 87, 112, 340
BssMI GATC 2 cut(s) 14, 321
Bst2UI CCWGG 1 cut(s) 243
BstC8I GCNNGC 1 cut(s) 52
BstF5I GGATG 1 cut(s) 238
BstHHI GCGC 1 cut(s) 334
BstKTI GATC 2 cut(s) 17, 324
BstMAI GTCTC 1 cut(s) 270
BstMBI GATC 2 cut(s) 14, 321
BstNI CCWGG 1 cut(s) 243
BstNSI RCATGY 1 cut(s) 47
BstSCI CCNGG 1 cut(s) 241
BstV1I GCAGC 1 cut(s) 172
BstV2I GAAGAC 1 cut(s) 336
BstX2I RGATCY 1 cut(s) 14
BstYI RGATCY 1 cut(s) 14
BsuI GTATCC 1 cut(s) 214
BtrI CACGTC 1 cut(s) 280
BtsCI GGATG 1 cut(s) 238
BtsI GCAGTG 1 cut(s) 76
BtsIMutI CAGTG 2 cut(s) 76, 105
BveI ACCTGC 1 cut(s) 128
Cac8I GCNNGC 1 cut(s) 52
CfoI GCGC 1 cut(s) 334
Cfr13I GGNCC 1 cut(s) 206
CseI GACGC 1 cut(s) 153
Csp6I GTAC 1 cut(s) 90
CviAII CATG 1 cut(s) 44
CviJI RGCY 4 cut(s) 54, 77, 158, 303
CviKI_1 RGCY 4 cut(s) 54, 77, 158, 303
CviQI GTAC 1 cut(s) 90
DpnI GATC 2 cut(s) 16, 323
DpnII GATC 2 cut(s) 14, 321
DraI TTTAAA 1 cut(s) 58
Eco47I GGWCC 1 cut(s) 206
Eco57I CTGAAG 1 cut(s) 144
EcoO109I RGGNCCY 1 cut(s) 206
EcoRII CCWGG 1 cut(s) 241
Esp3I CGTCTC 1 cut(s) 270
FaeI CATG 1 cut(s) 47
FaqI GGGAC 1 cut(s) 166
FatI CATG 1 cut(s) 43
FauNDI CATATG 1 cut(s) 215
Fnu4HI GCNGC 1 cut(s) 186
FokI GGATG 1 cut(s) 225
Fsp4HI GCNGC 1 cut(s) 186
FspBI CTAG 2 cut(s) 26, 126
GlaI GCGC 1 cut(s) 333
GluI GCNGC 1 cut(s) 186
GsaI CCCAGC 2 cut(s) 137, 213
HgaI GACGC 1 cut(s) 153
HhaI GCGC 1 cut(s) 334
Hin1II CATG 1 cut(s) 47
Hin6I GCGC 1 cut(s) 332
HinP1I GCGC 1 cut(s) 332
Hpy166II GTNNAC 1 cut(s) 285
Hpy188I TCNGA 1 cut(s) 321
Hpy8I GTNNAC 1 cut(s) 285
HpyAV CCTTC 2 cut(s) 173, 188
HpyCH4IV ACGT 1 cut(s) 279
HpyCH4V TGCA 2 cut(s) 10, 50
HpySE526I ACGT 1 cut(s) 279
Hsp92II CATG 1 cut(s) 47
HspAI GCGC 1 cut(s) 332
Kzo9I GATC 2 cut(s) 14, 321
Lsp1109I GCAGC 1 cut(s) 172
MaeI CTAG 2 cut(s) 26, 126
MaeII ACGT 1 cut(s) 279
MalI GATC 2 cut(s) 16, 323
MboI GATC 2 cut(s) 14, 321
MboII GAAGA 2 cut(s) 308, 336
MflI RGATCY 1 cut(s) 14
MluCI AATT 1 cut(s) 59
MnlI CCTC 2 cut(s) 125, 197
MseI TTAA 1 cut(s) 57
MslI CAYNNNNRTG 2 cut(s) 243, 290
MspR9I CCNGG 1 cut(s) 243
MvaI CCWGG 1 cut(s) 243
NdeI CATATG 1 cut(s) 215
NdeII GATC 2 cut(s) 14, 321
NlaIII CATG 1 cut(s) 47
NlaIV GGNNCC 2 cut(s) 208, 247
NspI RCATGY 1 cut(s) 47
PciI ACATGT 1 cut(s) 43
PflMI CCANNNNNTGG 1 cut(s) 216
PkrI GCNGC 1 cut(s) 187
PpuMI RGGWCCY 1 cut(s) 206
PscI ACATGT 1 cut(s) 43
Psp5II RGGWCCY 1 cut(s) 206
Psp6I CCWGG 1 cut(s) 241
PspFI CCCAGC 2 cut(s) 133, 209
PspGI CCWGG 1 cut(s) 241
PspN4I GGNNCC 2 cut(s) 208, 247
PspPI GGNCC 1 cut(s) 206
PspPPI RGGWCCY 1 cut(s) 206
PsuI RGATCY 1 cut(s) 14
RsaI GTAC 1 cut(s) 91
RsaNI GTAC 1 cut(s) 90
RseI CAYNNNNRTG 2 cut(s) 243, 290
SaqAI TTAA 1 cut(s) 57
SatI GCNGC 1 cut(s) 186
Sau3AI GATC 2 cut(s) 14, 321
Sau96I GGNCC 1 cut(s) 206
ScaI AGTACT 1 cut(s) 91
ScrFI CCNGG 1 cut(s) 243
SetI ASST 9 cut(s) 79, 142, 160, 184, 199, 227, 247, 282, 356
SinI GGWCC 1 cut(s) 206
SmiMI CAYNNNNRTG 2 cut(s) 243, 290
SpeI ACTAGT 1 cut(s) 125
Sse9I AATT 1 cut(s) 59
SspMI CTAG 2 cut(s) 26, 126
StyD4I CCNGG 1 cut(s) 241
TaiI ACGT 1 cut(s) 282
TasI AATT 1 cut(s) 59
TatI WGTACW 1 cut(s) 89
Tru1I TTAA 1 cut(s) 57
Tru9I TTAA 1 cut(s) 57
TscAI CASTG 2 cut(s) 76, 112
TseI GCWGC 1 cut(s) 185
TspDTI ATGAA 2 cut(s) 133, 192
TspRI CASTG 2 cut(s) 76, 112
Van91I CCANNNNNTGG 1 cut(s) 216
VpaK11BI GGWCC 1 cut(s) 206
XceI RCATGY 1 cut(s) 47
XspI CTAG 2 cut(s) 26, 126
ZrmI AGTACT 1 cut(s) 91
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.