Rh1BG001300

Belongs to the glycosyl hydrolase 1 family

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
rosa_samantha
Chr1B
OG0010589
Physical Location & Seq
Forward (+)
158798 .. 159308
511 bp
Loading structure...
UTR
Exon/CDS
Intron
Rh1BG001300.1

Sequence Viewer

Length: 345 bp
ATGGGTGGTCGTCCTCTGCTCTTCTTCATCTTGATCTTGATCTGCCTCATACTCACTGCTCTTGTTGGCTCATCAGCAAGCAATGAGGCGGCTGCTGCTGCTGCTCCTCGTCATTATTCGATTCCATTCAATAGAAGTAGCTTCCCAGCTAGTTTCATCTTCGGAGCTGGTTCTGCTGCTTATCAGACTGAAGGAGCAGCACATATCCATGGAAAGGGACCAAGCATATGGGACACTTTCACTACAAAACATCAAGGTTCAATTCCTCTCCATCATATACGCCATCGAGCAATCTTTCATTTCCAAATGCAGTCTTCTGTACATTACTTTGCTTTTGATCGATAA

Protein Analysis

114

Amino Acids

12.47

Weight (kDa)

9.85

Isoelectric Point (pI)

54.3

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
Glyco_hydro_1 PF00232 46 - 100 2.5e-12 Glycosyl hydrolase family 1
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

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Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AciI CCGC 1 cut(s) 89
AcuI CTGAAG 1 cut(s) 210
AfaI GTAC 1 cut(s) 321
AfiI CCNNNNNNNGG 1 cut(s) 214
AgsI TTSAA 2 cut(s) 130, 261
AluBI AGCT 3 cut(s) 141, 149, 167
AluI AGCT 3 cut(s) 141, 149, 167
ApeKI GCWGC 6 cut(s) 92, 95, 98, 101, 176, 197
AspS9I GGNCC 1 cut(s) 218
AvaII GGWCC 1 cut(s) 218
BbsI GAAGAC 1 cut(s) 306
BbvI GCAGC 6 cut(s) 79, 82, 85, 88, 163, 209
BccI CCATC 2 cut(s) 279, 291
BfaI CTAG 1 cut(s) 150
BisI GCNGC 7 cut(s) 90, 93, 96, 99, 102, 177, 198
BlsI GCNGC 7 cut(s) 91, 94, 97, 100, 103, 178, 199
Bme18I GGWCC 1 cut(s) 218
BmgT120I GGNCC 1 cut(s) 218
BmiI GGNNCC 1 cut(s) 219
BpiI GAAGAC 1 cut(s) 306
Bsa29I ATCGAT 1 cut(s) 340
BsaBI GATNNNNATC 1 cut(s) 38
BsaJI CCNNGG 1 cut(s) 208
Bsc4I CCNNNNNNNGG 1 cut(s) 214
Bse3DI GCAATG 1 cut(s) 88
Bse8I GATNNNNATC 1 cut(s) 38
BseCI ATCGAT 1 cut(s) 340
BseDI CCNNGG 1 cut(s) 208
BseJI GATNNNNATC 1 cut(s) 38
BseLI CCNNNNNNNGG 1 cut(s) 214
BseMI GCAATG 1 cut(s) 88
BseRI GAGGAG 1 cut(s) 96
BseXI GCAGC 6 cut(s) 79, 82, 85, 88, 163, 209
BseYI CCCAGC 1 cut(s) 145
BshVI ATCGAT 1 cut(s) 340
BslFI GGGAC 2 cut(s) 231, 245
BslI CCNNNNNNNGG 1 cut(s) 214
BsmFI GGGAC 2 cut(s) 231, 245
Bsp1407I TGTACA 1 cut(s) 319
Bsp143I GATC 3 cut(s) 33, 39, 337
Bsp19I CCATGG 1 cut(s) 208
BspACI CCGC 1 cut(s) 89
BspDI ATCGAT 1 cut(s) 340
BspLI GGNNCC 1 cut(s) 219
BspQI GCTCTTC 1 cut(s) 26
BsrDI GCAATG 1 cut(s) 88
BsrGI TGTACA 1 cut(s) 319
BssECI CCNNGG 1 cut(s) 208
BssMI GATC 3 cut(s) 33, 39, 337
BssT1I CCWWGG 1 cut(s) 208
Bst6I CTCTTC 1 cut(s) 26
BstAUI TGTACA 1 cut(s) 319
BstC8I GCNNGC 1 cut(s) 79
BstDSI CCRYGG 1 cut(s) 208
BstKTI GATC 3 cut(s) 36, 42, 340
BstMBI GATC 3 cut(s) 33, 39, 337
BstMWI GCNNNNNNNGC 4 cut(s) 95, 98, 101, 173
BstV1I GCAGC 6 cut(s) 79, 82, 85, 88, 163, 209
BstV2I GAAGAC 1 cut(s) 306
BstXI CCANNNNNNTGG 1 cut(s) 228
Bsu15I ATCGAT 1 cut(s) 340
BsuTUI ATCGAT 1 cut(s) 340
BtgI CCRYGG 1 cut(s) 208
BtsI GCAGTG 1 cut(s) 54
BtsIMutI CAGTG 1 cut(s) 54
Cac8I GCNNGC 1 cut(s) 79
Cfr13I GGNCC 1 cut(s) 218
ClaI ATCGAT 1 cut(s) 340
Csp6I GTAC 1 cut(s) 320
CviAII CATG 1 cut(s) 209
CviJI RGCY 5 cut(s) 69, 92, 141, 149, 167
CviKI_1 RGCY 5 cut(s) 69, 92, 141, 149, 167
CviQI GTAC 1 cut(s) 320
DpnI GATC 3 cut(s) 35, 41, 339
DpnII GATC 3 cut(s) 33, 39, 337
Eam1104I CTCTTC 1 cut(s) 26
EarI CTCTTC 1 cut(s) 26
Eco130I CCWWGG 1 cut(s) 208
Eco47I GGWCC 1 cut(s) 218
Eco57I CTGAAG 1 cut(s) 210
EcoT14I CCWWGG 1 cut(s) 208
ErhI CCWWGG 1 cut(s) 208
FaeI CATG 1 cut(s) 212
FaiI YATR 7 cut(s) 50, 204, 210, 227, 229, 276, 278
FaqI GGGAC 2 cut(s) 231, 245
FatI CATG 1 cut(s) 208
FauNDI CATATG 1 cut(s) 227
Fnu4HI GCNGC 7 cut(s) 90, 93, 96, 99, 102, 177, 198
Fsp4HI GCNGC 7 cut(s) 90, 93, 96, 99, 102, 177, 198
FspBI CTAG 1 cut(s) 150
GluI GCNGC 7 cut(s) 90, 93, 96, 99, 102, 177, 198
GsaI CCCAGC 1 cut(s) 149
Hin1II CATG 1 cut(s) 212
HinfI GANTC 1 cut(s) 121
Hpy188I TCNGA 2 cut(s) 164, 186
Hpy188III TCNNGA 2 cut(s) 31, 37
HpyAV CCTTC 1 cut(s) 185
HpyCH4V TGCA 1 cut(s) 310
HpyF10VI GCNNNNNNNGC 4 cut(s) 95, 98, 101, 173
Hsp92II CATG 1 cut(s) 212
Kzo9I GATC 3 cut(s) 33, 39, 337
LguI GCTCTTC 1 cut(s) 26
LmnI GCTCC 3 cut(s) 109, 164, 194
LpnPI CCDG 2 cut(s) 153, 159
Lsp1109I GCAGC 6 cut(s) 79, 82, 85, 88, 163, 209
MaeI CTAG 1 cut(s) 150
MalI GATC 3 cut(s) 35, 41, 339
MboI GATC 3 cut(s) 33, 39, 337
MboII GAAGA 4 cut(s) 13, 16, 151, 306
MluCI AATT 1 cut(s) 261
MnlI CCTC 5 cut(s) 24, 56, 79, 117, 276
MslI CAYNNNNRTG 1 cut(s) 207
MwoI GCNNNNNNNGC 4 cut(s) 95, 98, 101, 173
NcoI CCATGG 1 cut(s) 208
NdeI CATATG 1 cut(s) 227
NdeII GATC 3 cut(s) 33, 39, 337
NlaIII CATG 1 cut(s) 212
NlaIV GGNNCC 1 cut(s) 219
PciSI GCTCTTC 1 cut(s) 26
PfeI GAWTC 1 cut(s) 121
PkrI GCNGC 7 cut(s) 91, 94, 97, 100, 103, 178, 199
PspFI CCCAGC 1 cut(s) 145
PspN4I GGNNCC 1 cut(s) 219
PspPI GGNCC 1 cut(s) 218
RsaI GTAC 1 cut(s) 321
RsaNI GTAC 1 cut(s) 320
RseI CAYNNNNRTG 1 cut(s) 207
SapI GCTCTTC 1 cut(s) 26
SatI GCNGC 7 cut(s) 90, 93, 96, 99, 102, 177, 198
Sau3AI GATC 3 cut(s) 33, 39, 337
Sau96I GGNCC 1 cut(s) 218
SetI ASST 4 cut(s) 143, 151, 169, 259
SinI GGWCC 1 cut(s) 218
SmiMI CAYNNNNRTG 1 cut(s) 207
Sse9I AATT 1 cut(s) 261
SsiI CCGC 1 cut(s) 89
SspMI CTAG 1 cut(s) 150
StyI CCWWGG 1 cut(s) 208
TaqI TCGA 3 cut(s) 119, 286, 340
TasI AATT 1 cut(s) 261
TatI WGTACW 1 cut(s) 319
TauI GCSGC 1 cut(s) 92
TfiI GAWTC 1 cut(s) 121
TscAI CASTG 1 cut(s) 61
TseI GCWGC 6 cut(s) 92, 95, 98, 101, 176, 197
TspDTI ATGAA 3 cut(s) 16, 145, 287
TspRI CASTG 1 cut(s) 61
VpaK11BI GGWCC 1 cut(s) 218
XspI CTAG 1 cut(s) 150
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.