Rh6BG198000

Belongs to the glycosyl hydrolase 1 family

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
rosa_samantha
Chr6B
OG0010589
Physical Location & Seq
Forward (+)
37248569 .. 37255581
7013 bp
Loading structure...
UTR
Exon/CDS
Intron
Rh6BG198000.1

Sequence Viewer

Length: 564 bp
ATGCAAGGATCTCTGTTTCTAGGCGTAGTGCTTCTACTTGTTGCAGGCTTAAGATTGACAAGCAGTGAAGCTCTTACACCCAGTACTCCTATCTATGACACTGGTTTCCTCAACAGGACTAGCTTCCCAGCAGGTTTCATATTTGGGACAGCTTCAGCGTCTTATCAGTATGAAGGTGCTGCTAAAGAAGGTGGTAGAGGGCCGAGCATATGGGATACCTACACCCACGAACATCCAGAACAGATGGCAGCCCAATTTGAAGAACAGTTGGATCATGCTAAAGGAGGAAAACGAAAAGTGACTAGTAGACGTGAAACGGGCTTTCGGAGTCCGATTGGGATGTACCTTACCTTTCCGAAAAGGGAATCGCGCCAGAAATCAAACGTGTTGCTTTGCCACGACCTGTGGTCTTTTTCGGGCTTTCGGGGTCGTTTAGGGCCTCAAAACTGCATTTTTCTATTTTTCGACGCTTTGGTTTTCCTAGTTATTTTCGGGTTGTTTTCATTTTTACCCATGGGCTTTAGGGTTTCATTGTTAGTCGCCCTAGGGTTTATTTTCTTATAA

Protein Analysis

187

Amino Acids

20.82

Weight (kDa)

9.26

Isoelectric Point (pI)

39.86

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
Glyco_hydro_1 PF00232 40 - 83 2e-13 Glycosyl hydrolase family 1
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

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Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AanI TTATAA 1 cut(s) 562
Acc36I ACCTGC 1 cut(s) 122
AccI GTMKAC 1 cut(s) 307
AccII CGCG 1 cut(s) 370
AclWI GGATC 2 cut(s) 16, 279
AcuI CTGAAG 1 cut(s) 138
AfaI GTAC 2 cut(s) 85, 344
AflII CTTAAG 1 cut(s) 49
AflIII ACRYGT 1 cut(s) 384
AgsI TTSAA 1 cut(s) 260
AhdI GACNNNNNGTC 1 cut(s) 406
AhlI ACTAGT 1 cut(s) 302
AjiI CACGTC 1 cut(s) 311
AluBI AGCT 3 cut(s) 71, 123, 152
AluI AGCT 3 cut(s) 71, 123, 152
AlwI GGATC 2 cut(s) 16, 279
AoxI GGCC 2 cut(s) 200, 437
ApeKI GCWGC 2 cut(s) 179, 248
AspA2I CCTAGG 1 cut(s) 544
AspLEI GCGC 1 cut(s) 372
AspS9I GGNCC 2 cut(s) 200, 437
AvrII CCTAGG 1 cut(s) 544
BbvI GCAGC 2 cut(s) 166, 260
BccI CCATC 1 cut(s) 238
BciVI GTATCC 1 cut(s) 208
BcuI ACTAGT 1 cut(s) 302
BfaI CTAG 5 cut(s) 20, 120, 303, 482, 545
BfrI CTTAAG 1 cut(s) 49
BfuAI ACCTGC 1 cut(s) 122
BfuI GTATCC 1 cut(s) 208
BisI GCNGC 2 cut(s) 180, 249
BlnI CCTAGG 1 cut(s) 544
BlsI GCNGC 2 cut(s) 181, 250
BmcAI AGTACT 1 cut(s) 85
BmeRI GACNNNNNGTC 1 cut(s) 406
BmgBI CACGTC 1 cut(s) 311
BmgT120I GGNCC 2 cut(s) 200, 437
BmrI ACTGGG 1 cut(s) 75
BmuI ACTGGG 1 cut(s) 75
BsaJI CCNNGG 2 cut(s) 513, 544
Bse1I ACTGG 2 cut(s) 81, 106
BseDI CCNNGG 2 cut(s) 513, 544
BseGI GGATG 2 cut(s) 232, 345
BseNI ACTGG 2 cut(s) 81, 106
BseXI GCAGC 2 cut(s) 166, 260
BseYI CCCAGC 1 cut(s) 127
Bsh1236I CGCG 1 cut(s) 370
BshFI GGCC 2 cut(s) 202, 439
BslFI GGGAC 1 cut(s) 160
BsmFI GGGAC 1 cut(s) 160
BsnI GGCC 2 cut(s) 202, 439
Bsp143I GATC 2 cut(s) 8, 271
Bsp19I CCATGG 1 cut(s) 513
BspANI GGCC 2 cut(s) 202, 439
BspFNI CGCG 1 cut(s) 370
BspMI ACCTGC 1 cut(s) 122
BspPI GGATC 2 cut(s) 16, 279
BspTI CTTAAG 1 cut(s) 49
BsrI ACTGG 2 cut(s) 81, 106
BssECI CCNNGG 2 cut(s) 513, 544
BssMI GATC 2 cut(s) 8, 271
BssT1I CCWWGG 2 cut(s) 513, 544
Bst4CI ACNGT 1 cut(s) 267
BstAFI CTTAAG 1 cut(s) 49
BstC8I GCNNGC 1 cut(s) 46
BstDSI CCRYGG 1 cut(s) 513
BstF5I GGATG 2 cut(s) 232, 345
BstFNI CGCG 1 cut(s) 370
BstHHI GCGC 1 cut(s) 372
BstKTI GATC 2 cut(s) 11, 274
BstMBI GATC 2 cut(s) 8, 271
BstUI CGCG 1 cut(s) 370
BstV1I GCAGC 2 cut(s) 166, 260
BstX2I RGATCY 1 cut(s) 8
BstYI RGATCY 1 cut(s) 8
BsuI GTATCC 1 cut(s) 208
BsuRI GGCC 2 cut(s) 202, 439
BtgI CCRYGG 1 cut(s) 513
BtrI CACGTC 1 cut(s) 311
BtsCI GGATG 2 cut(s) 232, 345
BtsI GCAGTG 1 cut(s) 70
BtsIMutI CAGTG 2 cut(s) 70, 99
BveI ACCTGC 1 cut(s) 122
Cac8I GCNNGC 1 cut(s) 46
CfoI GCGC 1 cut(s) 372
Cfr13I GGNCC 2 cut(s) 200, 437
CseI GACGC 2 cut(s) 147, 476
Csp6I GTAC 2 cut(s) 84, 343
CviAII CATG 2 cut(s) 275, 514
CviQI GTAC 2 cut(s) 84, 343
DpnI GATC 2 cut(s) 10, 273
DpnII GATC 2 cut(s) 8, 271
DriI GACNNNNNGTC 1 cut(s) 406
Eam1105I GACNNNNNGTC 1 cut(s) 406
Eco130I CCWWGG 2 cut(s) 513, 544
Eco57I CTGAAG 1 cut(s) 138
EcoO109I RGGNCCY 1 cut(s) 437
EcoT14I CCWWGG 2 cut(s) 513, 544
ErhI CCWWGG 2 cut(s) 513, 544
FaeI CATG 2 cut(s) 278, 517
FaiI YATR 8 cut(s) 96, 140, 171, 209, 211, 276, 515, 562
FaqI GGGAC 1 cut(s) 160
FatI CATG 2 cut(s) 274, 513
FauNDI CATATG 1 cut(s) 209
FblI GTMKAC 1 cut(s) 307
Fnu4HI GCNGC 2 cut(s) 180, 249
FokI GGATG 2 cut(s) 219, 352
Fsp4HI GCNGC 2 cut(s) 180, 249
FspBI CTAG 5 cut(s) 20, 120, 303, 482, 545
GlaI GCGC 1 cut(s) 371
GluI GCNGC 2 cut(s) 180, 249
GsaI CCCAGC 1 cut(s) 131
HaeIII GGCC 2 cut(s) 202, 439
HgaI GACGC 2 cut(s) 147, 476
HhaI GCGC 1 cut(s) 372
Hin1II CATG 2 cut(s) 278, 517
Hin6I GCGC 1 cut(s) 370
HinP1I GCGC 1 cut(s) 370
HinfI GANTC 2 cut(s) 328, 365
Hpy166II GTNNAC 1 cut(s) 308
Hpy188I TCNGA 3 cut(s) 327, 333, 357
Hpy188III TCNNGA 1 cut(s) 236
Hpy8I GTNNAC 1 cut(s) 308
Hpy99I CGWCG 1 cut(s) 470
HpyAV CCTTC 2 cut(s) 167, 182
HpyCH4III ACNGT 1 cut(s) 267
HpyCH4IV ACGT 2 cut(s) 310, 384
HpyCH4V TGCA 3 cut(s) 4, 44, 450
HpySE526I ACGT 2 cut(s) 310, 384
Hsp92II CATG 2 cut(s) 278, 517
HspAI GCGC 1 cut(s) 370
Kzo9I GATC 2 cut(s) 8, 271
LpnPI CCDG 9 cut(s) 30, 87, 94, 100, 117, 141, 249, 386, 416
Lsp1109I GCAGC 2 cut(s) 166, 260
MaeI CTAG 5 cut(s) 20, 120, 303, 482, 545
MaeII ACGT 2 cut(s) 310, 384
MaeIII GTNAC 1 cut(s) 298
MalI GATC 2 cut(s) 10, 273
MboI GATC 2 cut(s) 8, 271
MboII GAAGA 1 cut(s) 272
MflI RGATCY 1 cut(s) 8
MluCI AATT 1 cut(s) 254
MlyI GAGTC 1 cut(s) 337
MmeI TCCRAC 1 cut(s) 249
MnlI CCTC 4 cut(s) 119, 191, 278, 450
MseI TTAA 1 cut(s) 50
MspCI CTTAAG 1 cut(s) 49
MvnI CGCG 1 cut(s) 370
NcoI CCATGG 1 cut(s) 513
NdeI CATATG 1 cut(s) 209
NdeII GATC 2 cut(s) 8, 271
NlaIII CATG 2 cut(s) 278, 517
NmeAIII GCCGAG 1 cut(s) 228
NmuCI GTSAC 1 cut(s) 298
PfeI GAWTC 1 cut(s) 365
PkrI GCNGC 2 cut(s) 181, 250
PleI GAGTC 1 cut(s) 336
PpsI GAGTC 1 cut(s) 336
PsiI TTATAA 1 cut(s) 562
PspFI CCCAGC 1 cut(s) 127
PspPI GGNCC 2 cut(s) 200, 437
PsuI RGATCY 1 cut(s) 8
RsaI GTAC 2 cut(s) 85, 344
RsaNI GTAC 2 cut(s) 84, 343
SaqAI TTAA 1 cut(s) 50
SatI GCNGC 2 cut(s) 180, 249
Sau3AI GATC 2 cut(s) 8, 271
Sau96I GGNCC 2 cut(s) 200, 437
ScaI AGTACT 1 cut(s) 85
SchI GAGTC 1 cut(s) 337
SmlI CTYRAG 1 cut(s) 49
SmoI CTYRAG 1 cut(s) 49
SpeI ACTAGT 1 cut(s) 302
Sse9I AATT 1 cut(s) 254
SspMI CTAG 5 cut(s) 20, 120, 303, 482, 545
StyI CCWWGG 2 cut(s) 513, 544
TaaI ACNGT 1 cut(s) 267
TaiI ACGT 2 cut(s) 313, 387
TaqI TCGA 1 cut(s) 465
TasI AATT 1 cut(s) 254
TatI WGTACW 1 cut(s) 83
TfiI GAWTC 1 cut(s) 365
Tru1I TTAA 1 cut(s) 50
Tru9I TTAA 1 cut(s) 50
TscAI CASTG 2 cut(s) 70, 106
TseFI GTSAC 1 cut(s) 298
TseI GCWGC 2 cut(s) 179, 248
Tsp45I GTSAC 1 cut(s) 298
TspDTI ATGAA 4 cut(s) 127, 186, 492, 519
TspRI CASTG 2 cut(s) 70, 106
Vha464I CTTAAG 1 cut(s) 49
XmaJI CCTAGG 1 cut(s) 544
XmiI GTMKAC 1 cut(s) 307
XspI CTAG 5 cut(s) 20, 120, 303, 482, 545
ZrmI AGTACT 1 cut(s) 85
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.