Rh5BG487900

Glycosyl hydrolase family 1

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
rosa_samantha
Chr5B
OG0010589
Physical Location & Seq
Reverse (-)
77758080 .. 77767836
9757 bp
Loading structure...
UTR
Exon/CDS
Intron
Rh5BG487900.1

Sequence Viewer

Length: 468 bp
ATGGCACTTCAAGGCACTCTCATCTCTGCAATCATACTTGTTTTTAGCTGTGCAGTGGCAACTAGTATAGCCACCGCCCCAAGCCATTACGACGTTGCTTCCATCAACCGGAGCACCTTTCCTGCAGGTTTCATATTTGGGACAGCTTCTTCAGCTTACCAGGTGCTGCAAAAGAAGATGGAAGAGGACCAAGCATATGGGACGCATACACTCACAAAATTCCAGGAGTATGGGATAAATCCAACAGATTGGAACTGTCAACGGCAACTATGGAAGGGTAGAACTGGAGCTGATCAAGGGGATGGCGTTAACGTGAAGGGATATTTTGCTTGGTCTTTGTTTGACAACTTCGAATGGAATATGGGTTACAGTGTTAGGTTTGGCATCAACTATGTGGATTACAATGATGGGCTCAAAAGATATCCTAAACTCTCAGCACACTGGTTCAAGAATTTTCTTGAAAATTAG

Protein Analysis

155

Amino Acids

17.51

Weight (kDa)

6.81

Isoelectric Point (pI)

30.39

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
Glyco_hydro_1 PF00232 101 - 154 1.2e-18 Glycosyl hydrolase family 1
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

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Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
Acc36I ACCTGC 1 cut(s) 116
AciI CCGC 1 cut(s) 75
AcsI RAATTY 2 cut(s) 218, 451
AcuI CTGAAG 1 cut(s) 135
AfiI CCNNNNNNNGG 1 cut(s) 108
AgsI TTSAA 3 cut(s) 11, 448, 461
AhlI ACTAGT 1 cut(s) 62
AjnI CCWGG 2 cut(s) 159, 222
AluBI AGCT 4 cut(s) 48, 146, 155, 290
AluI AGCT 4 cut(s) 48, 146, 155, 290
Alw21I GWGCWC 1 cut(s) 116
AlwNI CAGNNNCTG 1 cut(s) 166
ApeKI GCWGC 1 cut(s) 166
ApoI RAATTY 2 cut(s) 218, 451
AspS9I GGNCC 1 cut(s) 187
AsuII TTCGAA 1 cut(s) 351
AvaII GGWCC 1 cut(s) 187
BanII GRGCYC 1 cut(s) 414
Bbv12I GWGCWC 1 cut(s) 116
BbvI GCAGC 1 cut(s) 153
BccI CCATC 4 cut(s) 110, 172, 296, 401
BceAI ACGGC 1 cut(s) 278
BciT130I CCWGG 2 cut(s) 161, 224
BclI TGATCA 1 cut(s) 292
BcuI ACTAGT 1 cut(s) 62
BfaI CTAG 1 cut(s) 63
BfmI CTRYAG 1 cut(s) 123
BfuAI ACCTGC 1 cut(s) 116
BisI GCNGC 1 cut(s) 167
BlsI GCNGC 1 cut(s) 168
Bme1390I CCNGG 2 cut(s) 161, 224
Bme18I GGWCC 1 cut(s) 187
BmgT120I GGNCC 1 cut(s) 187
BmrFI CCNGG 2 cut(s) 161, 224
BmsI GCATC 1 cut(s) 393
BpmI CTGGAG 1 cut(s) 306
Bpu14I TTCGAA 1 cut(s) 351
BsaWI WCCGGW 1 cut(s) 108
Bsc4I CCNNNNNNNGG 1 cut(s) 108
Bse1I ACTGG 2 cut(s) 289, 446
BseBI CCWGG 2 cut(s) 161, 224
BseGI GGATG 1 cut(s) 307
BseLI CCNNNNNNNGG 1 cut(s) 108
BseMII CTCAG 1 cut(s) 447
BseNI ACTGG 2 cut(s) 289, 446
BseXI GCAGC 1 cut(s) 153
BsgI GTGCAG 1 cut(s) 72
BsiHKAI GWGCWC 1 cut(s) 116
BsiSI CCGG 1 cut(s) 109
BslFI GGGAC 2 cut(s) 154, 214
BslI CCNNNNNNNGG 1 cut(s) 108
BsmFI GGGAC 2 cut(s) 154, 214
Bsp119I TTCGAA 1 cut(s) 351
Bsp1286I GDGCHC 2 cut(s) 116, 414
Bsp143I GATC 1 cut(s) 292
BspACI CCGC 1 cut(s) 75
BspCNI CTCAG 1 cut(s) 446
BspMAI CTGCAG 1 cut(s) 127
BspMI ACCTGC 1 cut(s) 116
BspT104I TTCGAA 1 cut(s) 351
BsrI ACTGG 2 cut(s) 289, 446
BssMI GATC 1 cut(s) 292
Bst2UI CCWGG 2 cut(s) 161, 224
Bst4CI ACNGT 2 cut(s) 257, 371
Bst6I CTCTTC 1 cut(s) 177
BstBI TTCGAA 1 cut(s) 351
BstDEI CTNAG 1 cut(s) 433
BstF5I GGATG 1 cut(s) 307
BstKTI GATC 1 cut(s) 295
BstMBI GATC 1 cut(s) 292
BstMWI GCNNNNNNNGC 1 cut(s) 152
BstNI CCWGG 2 cut(s) 161, 224
BstSCI CCNGG 2 cut(s) 159, 222
BstSFI CTRYAG 1 cut(s) 123
BstV1I GCAGC 1 cut(s) 153
BstXI CCANNNNNNTGG 3 cut(s) 197, 230, 249
BtsCI GGATG 1 cut(s) 307
BtsI GCAGTG 1 cut(s) 60
BtsIMutI CAGTG 3 cut(s) 60, 376, 439
BveI ACCTGC 1 cut(s) 116
CaiI CAGNNNCTG 1 cut(s) 166
Cfr13I GGNCC 1 cut(s) 187
CseI GACGC 1 cut(s) 211
CsiI ACCWGGT 1 cut(s) 159
CviJI RGCY 7 cut(s) 48, 71, 84, 146, 155, 290, 412
CviKI_1 RGCY 7 cut(s) 48, 71, 84, 146, 155, 290, 412
DdeI CTNAG 1 cut(s) 433
DpnI GATC 1 cut(s) 294
DpnII GATC 1 cut(s) 292
Eam1104I CTCTTC 1 cut(s) 177
EarI CTCTTC 1 cut(s) 177
Eco24I GRGCYC 1 cut(s) 414
Eco32I GATATC 1 cut(s) 422
Eco47I GGWCC 1 cut(s) 187
Eco57I CTGAAG 1 cut(s) 135
EcoRII CCWGG 2 cut(s) 159, 222
EcoRV GATATC 1 cut(s) 422
EcoT38I GRGCYC 1 cut(s) 414
FaqI GGGAC 2 cut(s) 154, 214
FauNDI CATATG 1 cut(s) 196
FbaI TGATCA 1 cut(s) 292
Fnu4HI GCNGC 1 cut(s) 167
FokI GGATG 1 cut(s) 314
FriOI GRGCYC 1 cut(s) 414
Fsp4HI GCNGC 1 cut(s) 167
FspBI CTAG 1 cut(s) 63
GluI GCNGC 1 cut(s) 167
GsuI CTGGAG 1 cut(s) 306
HapII CCGG 1 cut(s) 109
HgaI GACGC 1 cut(s) 211
HincII GTYRAC 2 cut(s) 260, 310
HindII GTYRAC 2 cut(s) 260, 310
HpaI GTTAAC 1 cut(s) 310
HpaII CCGG 1 cut(s) 109
Hpy166II GTNNAC 2 cut(s) 260, 310
Hpy188III TCNNGA 2 cut(s) 448, 458
Hpy8I GTNNAC 2 cut(s) 260, 310
Hpy99I CGWCG 1 cut(s) 95
HpyAV CCTTC 2 cut(s) 268, 310
HpyCH4III ACNGT 2 cut(s) 257, 371
HpyCH4IV ACGT 2 cut(s) 93, 312
HpyCH4V TGCA 4 cut(s) 29, 53, 125, 169
HpyF10VI GCNNNNNNNGC 1 cut(s) 152
HpyF3I CTNAG 1 cut(s) 433
HpySE526I ACGT 2 cut(s) 93, 312
Ksp22I TGATCA 1 cut(s) 292
KspAI GTTAAC 1 cut(s) 310
Kzo9I GATC 1 cut(s) 292
LmnI GCTCC 2 cut(s) 111, 287
LpnPI CCDG 9 cut(s) 111, 122, 135, 146, 173, 209, 236, 270, 427
Lsp1109I GCAGC 1 cut(s) 153
LweI GCATC 1 cut(s) 393
MabI ACCWGGT 1 cut(s) 159
MaeI CTAG 1 cut(s) 63
MaeII ACGT 2 cut(s) 93, 312
MaeIII GTNAC 1 cut(s) 365
MalI GATC 1 cut(s) 294
MboI GATC 1 cut(s) 292
MboII GAAGA 3 cut(s) 141, 187, 194
MhlI GDGCHC 2 cut(s) 116, 414
MluCI AATT 3 cut(s) 218, 451, 463
MmeI TCCRAC 1 cut(s) 266
MnlI CCTC 1 cut(s) 178
MseI TTAA 1 cut(s) 309
MspI CCGG 1 cut(s) 109
MspR9I CCNGG 2 cut(s) 161, 224
MvaI CCWGG 2 cut(s) 161, 224
MwoI GCNNNNNNNGC 1 cut(s) 152
NdeI CATATG 1 cut(s) 196
NdeII GATC 1 cut(s) 292
NspV TTCGAA 1 cut(s) 351
PfoI TCCNGGA 1 cut(s) 222
PkrI GCNGC 1 cut(s) 168
Psp6I CCWGG 2 cut(s) 159, 222
PspGI CCWGG 2 cut(s) 159, 222
PspPI GGNCC 1 cut(s) 187
PstI CTGCAG 1 cut(s) 127
PstNI CAGNNNCTG 1 cut(s) 166
SaqAI TTAA 1 cut(s) 309
SatI GCNGC 1 cut(s) 167
Sau3AI GATC 1 cut(s) 292
Sau96I GGNCC 1 cut(s) 187
SbfI CCTGCAGG 1 cut(s) 127
ScrFI CCNGG 2 cut(s) 161, 224
SdaI CCTGCAGG 1 cut(s) 127
SduI GDGCHC 2 cut(s) 116, 414
SexAI ACCWGGT 1 cut(s) 159
SfaNI GCATC 1 cut(s) 393
SfcI CTRYAG 1 cut(s) 123
SfuI TTCGAA 1 cut(s) 351
SinI GGWCC 1 cut(s) 187
SpeI ACTAGT 1 cut(s) 62
Sse8387I CCTGCAGG 1 cut(s) 127
Sse9I AATT 3 cut(s) 218, 451, 463
SsiI CCGC 1 cut(s) 75
SspMI CTAG 1 cut(s) 63
StyD4I CCNGG 2 cut(s) 159, 222
TaaI ACNGT 2 cut(s) 257, 371
TaiI ACGT 2 cut(s) 96, 315
TaqI TCGA 1 cut(s) 351
TasI AATT 3 cut(s) 218, 451, 463
Tru1I TTAA 1 cut(s) 309
Tru9I TTAA 1 cut(s) 309
TscAI CASTG 3 cut(s) 60, 376, 446
TseI GCWGC 1 cut(s) 166
TspDTI ATGAA 1 cut(s) 121
TspRI CASTG 3 cut(s) 60, 376, 446
VpaK11BI GGWCC 1 cut(s) 187
XapI RAATTY 2 cut(s) 218, 451
XspI CTAG 1 cut(s) 63
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.