pycom11g02420

Lectin-domain containing receptor kinase

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
pyrus_communis
Chr11
OG0005408
Physical Location & Seq
Forward (+)
2048140 .. 2049882
1743 bp
Loading structure...
UTR
Exon/CDS
Intron
N/A

Sequence Viewer

Length: 1326 bp
ATGGGACCATGCATGTTTAGGATTTGTTTAGGATTTGATTTGGGGTTTCTTGGGTTAGCCAATTTTGAGTCAAGGTTAACGTGTTTTTGTACAAAACTAATTAAGAAAACACTCTACTTACATTATATTATCAAATTATCGAATTTGATACTAAATAATTATACAGAATTATACTTTACCAAAGCTTTCTTCAATGATAAACTAGTAAACCTCACACACTATCAAAAAAAGTTTGCATTCGTGAATAAACTTGGCATACGGGTCTTGTTTGTGTCATCCTGTTATATCTTAATGGGTCTTGTTGTGTCAGATCATTATCTTAACGTGTTCTTAACAGGCAAGAAATTGTTATATCTAATATCAAGATCTAGCAAAAGATGTCTTATCAAGTTCTTAATAGTTGATCAAAAACCTGTTATTTTTATGTCATTTCGTGTTAGATTAATAGTTGATGTTATTATATTGACAATATACGCTGTCGTTTTGAGATTCCCCCACCCCGAAAAGTCCCTCATAACCGTTGCAAACCCTCTCTCAGTACCCAACCGTTTAAACCCCACTCCCTCCCTCCCTCCCTCTCTCTCTCTCTCTCTCTCTCTCTCTCTCTCTCTACATGTTCTCAGTCAAAATCTCCAAGTCCTCTGCTTTGGCAAAGAAAATCGAAAACCCATTTGTTTCCATCCATCAGAACTCAAAATCTCCATATTCATGTCTCCAAGAAAGCTTCTTGTTTTCTGGGTCGACGTCTTCTTCCTCATAACTCTCTTCCTCCGCCGTGGCTCTGCCGAGAACGTCAACTTCTACTTCCCATCTTTGAACTTCCAAAACCTTACCTTCCTCGGCGACTTCCACATCCGAAATGGTGTCGTGGGGCTCACCCGCCAGCTCACCGTCCCCTTCTCCAGCGCCGGTGCCGTCATCTACAACAACCCGATTCGGTTCTTCGACCCGGAGTCCAACATCACTGCTTCTTTCTCCGCAAAGTTCGCTTTCTCCATCGCCAACGTTAATCCGAGCTCCTACGGCGACGGCATATCGTTTTTCCTCTCGCTGGACAACTGGACTCTGGGCAGCCCTGGTGGATGTCTGGGTCTTGTCAATTCCTCGCAATTGACCAAGAACAAGTTCGTAGTCGTCGAATTTGACGCGAAGCTCGATTTTCACTTCGACGATCCGAACGACAATCACGTCGGATTGGACATTGAGAGCCTCAATTCGATCAAGACCTCTGATCCGATTCACAGGGTATCGATTTCAAGGGCGGGAATTCGATTACTGCTTGGATTGATTACAATAACGACCAAGAAAAGCTTAAAGTGTATCTAA

Protein Analysis

442

Amino Acids

49.79

Weight (kDa)

9.59

Isoelectric Point (pI)

36.91

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
Lectin_legB PF00139 265 - 411 3.6e-32 Legume lectin domain
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

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Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AasI GACNNNNNNGTC 1 cut(s) 1187
AatII GACGTC 1 cut(s) 747
AccB1I GGYRCC 1 cut(s) 911
AccI GTMKAC 1 cut(s) 741
AccII CGCG 1 cut(s) 1148
AciI CCGC 4 cut(s) 772, 880, 978, 1262
AclI AACGTT 1 cut(s) 1005
AclWI GGATC 2 cut(s) 1166, 1226
AcsI RAATTY 3 cut(s) 142, 1139, 1266
AcyI GRCGYC 1 cut(s) 744
AfaI GTAC 2 cut(s) 91, 540
AfiI CCNNNNNNNGG 1 cut(s) 1051
AflIII ACRYGT 3 cut(s) 80, 324, 613
AgsI TTSAA 3 cut(s) 193, 817, 1257
AhdI GACNNNNNGTC 1 cut(s) 952
AhlI ACTAGT 1 cut(s) 202
AjiI CACGTC 1 cut(s) 1189
AjnI CCWGG 1 cut(s) 1075
AjuI GAANNNNNNNTTGG 4 cut(s) 173, 205, 1109, 1141
AluBI AGCT 6 cut(s) 185, 724, 886, 1017, 1153, 1311
AluI AGCT 6 cut(s) 185, 724, 886, 1017, 1153, 1311
Alw21I GWGCWC 1 cut(s) 1019
Alw26I GTCTC 1 cut(s) 717
AlwI GGATC 2 cut(s) 1166, 1226
ApeKI GCWGC 1 cut(s) 1071
ApoI RAATTY 3 cut(s) 142, 1139, 1266
AseI ATTAAT 1 cut(s) 443
Asp700I GAANNNNTTC 1 cut(s) 1124
AspLEI GCGC 1 cut(s) 908
AspS9I GGNCC 1 cut(s) 5
AsuC2I CCSGG 1 cut(s) 950
AsuHPI GGTGA 2 cut(s) 868, 880
AvaII GGWCC 1 cut(s) 5
BanI GGYRCC 1 cut(s) 911
BanII GRGCYC 2 cut(s) 876, 1019
BbsI GAAGAC 1 cut(s) 739
Bbv12I GWGCWC 1 cut(s) 1019
BbvI GCAGC 1 cut(s) 1083
BccI CCATC 4 cut(s) 687, 691, 817, 1004
BceAI ACGGC 4 cut(s) 759, 899, 1039, 1045
BciT130I CCWGG 1 cut(s) 1077
BclI TGATCA 1 cut(s) 403
BcnI CCSGG 1 cut(s) 950
BcoDI GTCTC 1 cut(s) 717
BcuI ACTAGT 1 cut(s) 202
BfaI CTAG 2 cut(s) 203, 369
BfoI RGCGCY 1 cut(s) 909
BglII AGATCT 1 cut(s) 365
BisI GCNGC 1 cut(s) 1072
BlsI GCNGC 1 cut(s) 1073
Bme1390I CCNGG 2 cut(s) 950, 1077
Bme18I GGWCC 1 cut(s) 5
BmeRI GACNNNNNGTC 1 cut(s) 952
BmgBI CACGTC 1 cut(s) 1189
BmgT120I GGNCC 1 cut(s) 5
BmiI GGNNCC 2 cut(s) 6, 913
BmrFI CCNGG 2 cut(s) 950, 1077
BpiI GAAGAC 1 cut(s) 739
BpmI CTGGAG 1 cut(s) 886
BpuMI CCSGG 1 cut(s) 950
Bsa29I ATCGAT 1 cut(s) 1250
BsaBI GATNNNNATC 1 cut(s) 315
BsaHI GRCGYC 1 cut(s) 744
BsaJI CCNNGG 3 cut(s) 775, 838, 1075
Bsc4I CCNNNNNNNGG 1 cut(s) 1051
Bse118I RCCGGY 1 cut(s) 908
Bse1I ACTGG 1 cut(s) 1064
Bse8I GATNNNNATC 1 cut(s) 315
BseBI CCWGG 1 cut(s) 1077
BseCI ATCGAT 1 cut(s) 1250
BseDI CCNNGG 3 cut(s) 775, 838, 1075
BseGI GGATG 4 cut(s) 275, 679, 852, 1088
BseJI GATNNNNATC 1 cut(s) 315
BseLI CCNNNNNNNGG 1 cut(s) 1051
BseMII CTCAG 2 cut(s) 549, 634
BseNI ACTGG 1 cut(s) 1064
BseXI GCAGC 1 cut(s) 1083
Bsh1236I CGCG 1 cut(s) 1148
BshNI GGYRCC 1 cut(s) 911
BshVI ATCGAT 1 cut(s) 1250
BsiHKAI GWGCWC 1 cut(s) 1019
BsiSI CCGG 2 cut(s) 909, 950
BslFI GGGAC 3 cut(s) 18, 493, 878
BslI CCNNNNNNNGG 1 cut(s) 1051
BsmAI GTCTC 1 cut(s) 717
BsmFI GGGAC 3 cut(s) 18, 493, 878
BsmI GAATGC 1 cut(s) 236
Bsp1286I GDGCHC 2 cut(s) 876, 1019
Bsp1407I TGTACA 1 cut(s) 89
Bsp143I GATC 6 cut(s) 310, 365, 403, 1171, 1218, 1231
BspACI CCGC 4 cut(s) 772, 880, 978, 1262
BspCNI CTCAG 2 cut(s) 548, 633
BspDI ATCGAT 1 cut(s) 1250
BspFNI CGCG 1 cut(s) 1148
BspLI GGNNCC 2 cut(s) 6, 913
BspPI GGATC 2 cut(s) 1166, 1226
BspT107I GGYRCC 1 cut(s) 911
BsrFI RCCGGY 1 cut(s) 908
BsrGI TGTACA 1 cut(s) 89
BsrI ACTGG 1 cut(s) 1064
BssAI RCCGGY 1 cut(s) 908
BssECI CCNNGG 3 cut(s) 775, 838, 1075
BssMI GATC 6 cut(s) 310, 365, 403, 1171, 1218, 1231
BssNI GRCGYC 1 cut(s) 744
Bst2UI CCWGG 1 cut(s) 1077
Bst4CI ACNGT 3 cut(s) 520, 548, 892
Bst6I CTCTTC 1 cut(s) 770
BstACI GRCGYC 1 cut(s) 744
BstAUI TGTACA 1 cut(s) 89
BstC8I GCNNGC 1 cut(s) 884
BstDEI CTNAG 2 cut(s) 535, 620
BstDSI CCRYGG 1 cut(s) 775
BstF5I GGATG 4 cut(s) 275, 679, 852, 1088
BstFNI CGCG 1 cut(s) 1148
BstH2I RGCGCY 1 cut(s) 909
BstHHI GCGC 1 cut(s) 908
BstKTI GATC 6 cut(s) 313, 368, 406, 1174, 1221, 1234
BstMAI GTCTC 1 cut(s) 717
BstMBI GATC 6 cut(s) 310, 365, 403, 1171, 1218, 1231
BstMWI GCNNNNNNNGC 2 cut(s) 986, 1023
BstNI CCWGG 1 cut(s) 1077
BstNSI RCATGY 2 cut(s) 16, 617
BstSCI CCNGG 2 cut(s) 948, 1075
BstUI CGCG 1 cut(s) 1148
BstV1I GCAGC 1 cut(s) 1083
BstV2I GAAGAC 1 cut(s) 739
BstX2I RGATCY 1 cut(s) 365
BstYI RGATCY 1 cut(s) 365
Bsu15I ATCGAT 1 cut(s) 1250
BsuTUI ATCGAT 1 cut(s) 1250
BtgI CCRYGG 1 cut(s) 775
BtgZI GCGATG 1 cut(s) 982
BtrI CACGTC 1 cut(s) 1189
BtsCI GGATG 4 cut(s) 275, 679, 852, 1088
BtsI GCAGTG 1 cut(s) 963
BtsIMutI CAGTG 1 cut(s) 963
Cac8I GCNNGC 1 cut(s) 884
CfoI GCGC 1 cut(s) 908
Cfr10I RCCGGY 1 cut(s) 908
Cfr13I GGNCC 1 cut(s) 5
ClaI ATCGAT 1 cut(s) 1250
CseI GACGC 1 cut(s) 1154
Csp6I GTAC 2 cut(s) 90, 539
CviAII CATG 4 cut(s) 9, 13, 614, 709
CviQI GTAC 2 cut(s) 90, 539
DdeI CTNAG 2 cut(s) 535, 620
DpnI GATC 6 cut(s) 312, 367, 405, 1173, 1220, 1233
DpnII GATC 6 cut(s) 310, 365, 403, 1171, 1218, 1231
DraI TTTAAA 1 cut(s) 552
DrdI GACNNNNNNGTC 1 cut(s) 1187
DriI GACNNNNNGTC 1 cut(s) 952
DseDI GACNNNNNNGTC 1 cut(s) 1187
Eam1104I CTCTTC 1 cut(s) 770
Eam1105I GACNNNNNGTC 1 cut(s) 952
EarI CTCTTC 1 cut(s) 770
EciI GGCGGA 1 cut(s) 761
Ecl136II GAGCTC 1 cut(s) 1017
Eco24I GRGCYC 2 cut(s) 876, 1019
Eco47I GGWCC 1 cut(s) 5
Eco53kI GAGCTC 1 cut(s) 1017
EcoICRI GAGCTC 1 cut(s) 1017
EcoRI GAATTC 1 cut(s) 1266
EcoRII CCWGG 1 cut(s) 1075
EcoT22I ATGCAT 1 cut(s) 14
EcoT38I GRGCYC 2 cut(s) 876, 1019
FaeI CATG 4 cut(s) 12, 16, 617, 712
FalI AAGNNNNNCTT 1 cut(s) 1295
FaqI GGGAC 3 cut(s) 18, 493, 878
FatI CATG 4 cut(s) 8, 12, 613, 708
FauI CCCGC 2 cut(s) 887, 1255
FbaI TGATCA 1 cut(s) 403
FblI GTMKAC 1 cut(s) 741
Fnu4HI GCNGC 1 cut(s) 1072
FokI GGATG 4 cut(s) 262, 666, 839, 1095
FriOI GRGCYC 2 cut(s) 876, 1019
Fsp4HI GCNGC 1 cut(s) 1072
FspBI CTAG 2 cut(s) 203, 369
GlaI GCGC 1 cut(s) 907
GluI GCNGC 1 cut(s) 1072
GsuI CTGGAG 1 cut(s) 886
HaeII RGCGCY 1 cut(s) 909
HapII CCGG 2 cut(s) 909, 950
HgaI GACGC 1 cut(s) 1154
HhaI GCGC 1 cut(s) 908
Hin1I GRCGYC 1 cut(s) 744
Hin1II CATG 4 cut(s) 12, 16, 617, 712
Hin6I GCGC 1 cut(s) 906
HinP1I GCGC 1 cut(s) 906
HincII GTYRAC 3 cut(s) 78, 742, 796
HindII GTYRAC 3 cut(s) 78, 742, 796
HindIII AAGCTT 3 cut(s) 183, 722, 1309
HinfI GANTC 6 cut(s) 68, 489, 934, 953, 1063, 1237
HpaI GTTAAC 1 cut(s) 78
HpaII CCGG 2 cut(s) 909, 950
HphI GGTGA 2 cut(s) 868, 880
Hpy166II GTNNAC 4 cut(s) 78, 208, 742, 796
Hpy188I TCNGA 8 cut(s) 310, 688, 857, 1014, 1176, 1193, 1231, 1236
Hpy188III TCNNGA 3 cut(s) 241, 363, 1222
Hpy8I GTNNAC 4 cut(s) 78, 208, 742, 796
Hpy99I CGWCG 5 cut(s) 746, 1031, 1139, 1172, 1193
HpyAV CCTTC 2 cut(s) 844, 907
HpyCH4III ACNGT 3 cut(s) 520, 548, 892
HpyCH4IV ACGT 6 cut(s) 80, 324, 744, 792, 1005, 1188
HpyCH4V TGCA 3 cut(s) 12, 236, 524
HpyF10VI GCNNNNNNNGC 2 cut(s) 986, 1023
HpyF3I CTNAG 2 cut(s) 535, 620
HpySE526I ACGT 6 cut(s) 80, 324, 744, 792, 1005, 1188
Hsp92I GRCGYC 1 cut(s) 744
Hsp92II CATG 4 cut(s) 12, 16, 617, 712
HspAI GCGC 1 cut(s) 906
Ksp22I TGATCA 1 cut(s) 403
KspAI GTTAAC 1 cut(s) 78
Kzo9I GATC 6 cut(s) 310, 365, 403, 1171, 1218, 1231
LmnI GCTCC 1 cut(s) 1022
Lsp1109I GCAGC 1 cut(s) 1083
MaeI CTAG 2 cut(s) 203, 369
MaeII ACGT 6 cut(s) 80, 324, 744, 792, 1005, 1188
MalI GATC 6 cut(s) 312, 367, 405, 1173, 1220, 1233
MboI GATC 6 cut(s) 310, 365, 403, 1171, 1218, 1231
MboII GAAGA 5 cut(s) 181, 739, 742, 757, 934
MfeI CAATTG 1 cut(s) 1109
MflI RGATCY 1 cut(s) 365
MhlI GDGCHC 2 cut(s) 876, 1019
MlyI GAGTC 3 cut(s) 77, 962, 1057
MmeI TCCRAC 2 cut(s) 981, 1171
Mph1103I ATGCAT 1 cut(s) 14
MroXI GAANNNNTTC 1 cut(s) 1124
MslI CAYNNNNRTG 1 cut(s) 707
MspI CCGG 2 cut(s) 909, 950
MspR9I CCNGG 2 cut(s) 950, 1077
MssI GTTTAAAC 1 cut(s) 552
MunI CAATTG 1 cut(s) 1109
Mva1269I GAATGC 1 cut(s) 236
MvaI CCWGG 1 cut(s) 1077
MvnI CGCG 1 cut(s) 1148
MwoI GCNNNNNNNGC 2 cut(s) 986, 1023
NciI CCSGG 1 cut(s) 950
NdeII GATC 6 cut(s) 310, 365, 403, 1171, 1218, 1231
NlaIII CATG 4 cut(s) 12, 16, 617, 712
NlaIV GGNNCC 2 cut(s) 6, 913
NmeAIII GCCGAG 2 cut(s) 811, 819
NsiI ATGCAT 1 cut(s) 14
NspI RCATGY 2 cut(s) 16, 617
PciI ACATGT 1 cut(s) 613
PcsI WCGNNNNNNNCGW 4 cut(s) 1134, 1152, 1176, 1185
PctI GAATGC 1 cut(s) 236
PdmI GAANNNNTTC 1 cut(s) 1124
PfeI GAWTC 3 cut(s) 489, 934, 1237
PkrI GCNGC 1 cut(s) 1073
PleI GAGTC 3 cut(s) 76, 961, 1057
PmeI GTTTAAAC 1 cut(s) 552
PpsI GAGTC 3 cut(s) 76, 961, 1057
PscI ACATGT 1 cut(s) 613
PshBI ATTAAT 1 cut(s) 443
Psp124BI GAGCTC 1 cut(s) 1019
Psp1406I AACGTT 1 cut(s) 1005
Psp6I CCWGG 1 cut(s) 1075
PspGI CCWGG 1 cut(s) 1075
PspN4I GGNNCC 2 cut(s) 6, 913
PspPI GGNCC 1 cut(s) 5
PsuI RGATCY 1 cut(s) 365
RsaI GTAC 2 cut(s) 91, 540
RsaNI GTAC 2 cut(s) 90, 539
RseI CAYNNNNRTG 1 cut(s) 707
SacI GAGCTC 1 cut(s) 1019
SalI GTCGAC 1 cut(s) 740
SatI GCNGC 1 cut(s) 1072
Sau3AI GATC 6 cut(s) 310, 365, 403, 1171, 1218, 1231
Sau96I GGNCC 1 cut(s) 5
SchI GAGTC 3 cut(s) 77, 962, 1057
ScrFI CCNGG 2 cut(s) 950, 1077
SduI GDGCHC 2 cut(s) 876, 1019
SgrAI CRCCGGYG 1 cut(s) 908
SinI GGWCC 1 cut(s) 5
SmiMI CAYNNNNRTG 1 cut(s) 707
SpeI ACTAGT 1 cut(s) 202
SsiI CCGC 4 cut(s) 772, 880, 978, 1262
SspMI CTAG 2 cut(s) 203, 369
SstI GAGCTC 1 cut(s) 1019
StyD4I CCNGG 2 cut(s) 948, 1075
TaaI ACNGT 3 cut(s) 520, 548, 892
TaiI ACGT 6 cut(s) 83, 327, 747, 795, 1008, 1191
TatI WGTACW 1 cut(s) 89
TfiI GAWTC 3 cut(s) 489, 934, 1237
TscAI CASTG 1 cut(s) 970
TseI GCWGC 1 cut(s) 1071
TspDTI ATGAA 1 cut(s) 697
TspRI CASTG 1 cut(s) 970
VpaK11BI GGWCC 1 cut(s) 5
VspI ATTAAT 1 cut(s) 443
XapI RAATTY 3 cut(s) 142, 1139, 1266
XceI RCATGY 2 cut(s) 16, 617
XcmI CCANNNNNNNNNTGG 1 cut(s) 857
XmiI GTMKAC 1 cut(s) 741
XmnI GAANNNNTTC 1 cut(s) 1124
XspI CTAG 2 cut(s) 203, 369
ZraI GACGTC 1 cut(s) 745
Zsp2I ATGCAT 1 cut(s) 14
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.