pycom11g05430

cinnamoyl-CoA reductase 1-like

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
pyrus_communis
Chr11
OG0003662
Physical Location & Seq
Forward (+)
4152735 .. 4156147
3413 bp
Loading structure...
UTR
Exon/CDS
Intron
N/A

Sequence Viewer

Length: 1791 bp
ATGTCGAAGCAGGGTGAGGTCGTATGCGTTACCGGTGGAAGCGGCTGCATCGGATCCTGGCTCGTCCGTCTCCTCCTCCACCGTGATTACACCGTCCACGCCACCGTCAAGGATCTCAAGGACGATGGTGAGACGAAGCATCTAGAAGCGTTAGTAGAGGGGGCAGAGTCGCGCCTCCGTCTCTTCCAGATCGACCTCCTCGACTACAACTCCATACTCGCCGCCGTCAATGGTTGCTCCGGCGTCTTCCACCTCGCCTCTCCCTGCATCATCGATCCAGTCCACGACCCCGAGAAGGAGCTCCTGGACCCGGCGATCAAAGGAACGCTCAATGTTCTGACGGCGGCGAAGCAGGCTGGGGTCGGCCGTGTGGTGCTAACGTCATCGACCTCCGCCATCACTCCCAGCCCAAGCTGGCCGCCCGATAAGGTCAAGGGCGAGGATTGCTGGACAGACACTGACTACTGCAAGCAGAAGGGATTGTGGTATCCATTATCGAAAACGCTGGCAGAGAAAGCTGCGTGGGAATTTGCCAAGGAGAAGGGGCTGGATGTGGTTGTTGTGAATCCGGGCGCAGTGATGGGCCCTGTTATCTCGCCCAGGCTCAATTCTAGCATGTTAATGCTCCTTCGCATTCTTGAGGGCTGCACTGAAACATATGAGGACTTCTTTATGGGATCTGTGCATTTTAAAGATGTTGCTCTCGCACACATTTTAGTGTATGAGAACAAGTCAGCGACCGGTAGGCACTTGTGTTTGGAAGCTATATCACGTTATGGTGACTTTGTGGTAAGTTTCGGTCCACAAAATGCCTTTGCAAATGAAATTGGGTGCATATCTTATGTTTTAGATGTTTGGTTTCCTGATAATTTTAGGTGGTGTGAGTGCCTGATTTTGAAAATGATCGAGCTAATATGTTTACCTTTGTGTAGTTTGCCAAAGGACACCCAACCGGGCTTGCTGAGGGAAAAGAACGGAGCGAAGAAGCTAATGAACTTGGGTTTAGAATTCATTTCCATGGATCAAATTATCAAGGATGCTGTTGAGAGCCTAAAGACTCTTGTACTAGTAGCGAGGACATGTAATCTGGTTGTTTCAACTCCTTTGAATATTCCATTTTCTGTTTCACACTTTAATCAGGGTATTAGAATATCAATGCATTATTTGACTGGAACGCTACGTGGTAGCTGTAAGTTAGAATATCAGTGCATTGTTAGTTCTATTAATCCTAATTTGGTTTCTCTTAAGTTTCATACAATGCTTGCTTCGACCAAGTGGGAGAATGTTGGAGTAAAATCCCGTTCTCACAAGATTTTGTGGTCTCACAATCATTGTAAGTTACGATCAATTGTTATTCTTGTTAAAGGGGTTCCTAATCCTGATTGGTTAAGGAGATATAGACATATTCAGTTACCTATTAGGAGTGAAGTTCCCTGCATTTTGCTAAGTCATCCTGGATCGATCAGTACAACATACAAGACTCTTTGCGCACCAAGCTTTCGTCCATGGTGTCTTCAGGTTAGTTACTTGTGTTTTAATCTATATAGGCTAATAAAGTTTTACAGCTCCGGCCTTGTTGGTGTTATTCTCATCATAGTAGCTTGCTATCTGCATAATTTGTTTTCGTGCATGAAGTCTCAACTCGGGAAAGATATTCAGATTTTGAGAAGAAGGTATACATTCACTCCTAAAGTTTTCGTTAATCCTTTCCCTCCACACAGTAAACTGCAGCATCAAGGCAAAATCTTGGTAGATCAAAGAATCATGCTTCATAAGATCAAAGAATCTTAA
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
No pathways identified.
Pfam Domains
Protein Families
Epimerase

Protein Analysis

597

Amino Acids

66.77

Weight (kDa)

8.88

Isoelectric Point (pI)

41.64

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
NmrA PF05368 7 - 130 8.1e-10 NmrA-like family
Epimerase PF01370 8 - 246 2.3e-24 NAD dependent epimerase/dehydratase family
Polysacc_synt_2 PF02719 8 - 136 5.3e-08 Polysaccharide biosynthesis protein
3Beta_HSD PF01073 10 - 240 3e-18 3-beta hydroxysteroid dehydrogenase/isomerase family
GDP_Man_Dehyd PF16363 10 - 132 1.4e-15 GDP-mannose 4,6 dehydratase
NAD_binding_4 PF07993 10 - 204 2.3e-09 Male sterility protein
NAD_binding_10 PF13460 12 - 192 1.1e-10 NAD(P)H-binding
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Publication-ready
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Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
Acc16I TGCGCA 1 cut(s) 1489
AccI GTMKAC 1 cut(s) 1676
AccII CGCG 1 cut(s) 172
AciI CCGC 5 cut(s) 42, 222, 344, 393, 419
AclWI GGATC 7 cut(s) 48, 61, 120, 269, 685, 1029, 1465
AcoI YGGCCR 2 cut(s) 364, 416
AcsI RAATTY 2 cut(s) 527, 1007
AcuI CTGAAG 1 cut(s) 1499
AcyI GRCGYC 1 cut(s) 243
AfaI GTAC 2 cut(s) 1065, 1468
AfiI CCNNNNNNNGG 2 cut(s) 295, 310
AflII CTTAAG 1 cut(s) 1244
AflIII ACRYGT 1 cut(s) 1079
AgeI ACCGGT 2 cut(s) 32, 740
AgsI TTSAA 3 cut(s) 898, 1098, 1108
AhlI ACTAGT 1 cut(s) 1066
AjnI CCWGG 4 cut(s) 56, 303, 599, 1453
Alw21I GWGCWC 1 cut(s) 303
Alw26I GTCTC 5 cut(s) 74, 125, 185, 1326, 1641
AlwI GGATC 7 cut(s) 48, 61, 120, 269, 685, 1029, 1465
AlwNI CAGNNNCTG 1 cut(s) 458
Ama87I CYCGRG 2 cut(s) 290, 1643
AoxI GGCC 4 cut(s) 364, 416, 583, 1570
ApaI GGGCCC 1 cut(s) 587
ApeKI GCWGC 4 cut(s) 45, 518, 645, 1729
ApoI RAATTY 2 cut(s) 527, 1007
AseI ATTAAT 1 cut(s) 1224
AsiGI ACCGGT 2 cut(s) 32, 740
AspLEI GCGC 3 cut(s) 174, 575, 1490
AspS9I GGNCC 4 cut(s) 307, 583, 584, 800
AsuC2I CCSGG 3 cut(s) 311, 570, 954
AsuHPI GGTGA 3 cut(s) 26, 140, 791
AvaI CYCGRG 2 cut(s) 290, 1643
AvaII GGWCC 2 cut(s) 307, 800
BaeGI GKGCMC 1 cut(s) 587
BamHI GGATCC 1 cut(s) 53
BanII GRGCYC 2 cut(s) 303, 587
BarI GAAGNNNNNNTAC 2 cut(s) 1660, 1692
BbsI GAAGAC 2 cut(s) 238, 1505
Bbv12I GWGCWC 1 cut(s) 303
BbvCI CCTCAGC 1 cut(s) 962
BbvI GCAGC 4 cut(s) 32, 505, 632, 1741
BccI CCATC 3 cut(s) 119, 404, 574
BceAI ACGGC 3 cut(s) 209, 351, 357
BciT130I CCWGG 4 cut(s) 58, 305, 601, 1455
BciVI GTATCC 1 cut(s) 498
BcnI CCSGG 3 cut(s) 311, 570, 954
BcoDI GTCTC 5 cut(s) 74, 125, 185, 1326, 1641
BcuI ACTAGT 1 cut(s) 1066
BfaI CTAG 3 cut(s) 143, 612, 1067
BfmI CTRYAG 1 cut(s) 1727
BfrI CTTAAG 1 cut(s) 1244
BfuI GTATCC 1 cut(s) 498
BisI GCNGC 8 cut(s) 43, 46, 222, 345, 419, 519, 646, 1730
BlsI GCNGC 8 cut(s) 44, 47, 223, 346, 420, 520, 647, 1731
Bme1390I CCNGG 7 cut(s) 58, 305, 311, 570, 601, 954, 1455
Bme18I GGWCC 2 cut(s) 307, 800
BmeT110I CYCGRG 2 cut(s) 290, 1643
BmgT120I GGNCC 4 cut(s) 307, 583, 584, 800
BmiI GGNNCC 4 cut(s) 55, 309, 585, 1371
BmrFI CCNGG 7 cut(s) 58, 305, 311, 570, 601, 954, 1455
BmsI GCATC 5 cut(s) 57, 148, 276, 1027, 1741
BpiI GAAGAC 2 cut(s) 238, 1505
Bpu10I CCTNAGC 1 cut(s) 962
BpuEI CTTGAG 2 cut(s) 101, 659
BpuMI CCSGG 3 cut(s) 311, 570, 954
Bsa29I ATCGAT 2 cut(s) 273, 1460
BsaAI YACGTR 1 cut(s) 1181
BsaHI GRCGYC 1 cut(s) 243
BsaI GGTCTC 1 cut(s) 1326
BsaJI CCNNGG 4 cut(s) 534, 599, 1017, 1505
BsaWI WCCGGW 2 cut(s) 32, 740
BsaXI ACNNNNNCTCC 4 cut(s) 194, 224, 1669, 1699
Bsc4I CCNNNNNNNGG 2 cut(s) 295, 310
Bse118I RCCGGY 2 cut(s) 32, 740
Bse1I ACTGG 2 cut(s) 278, 1174
BseBI CCWGG 4 cut(s) 58, 305, 601, 1455
BseCI ATCGAT 2 cut(s) 273, 1460
BseDI CCNNGG 4 cut(s) 534, 599, 1017, 1505
BseGI GGATG 3 cut(s) 556, 1042, 1450
BseLI CCNNNNNNNGG 2 cut(s) 295, 310
BseMII CTCAG 1 cut(s) 953
BseNI ACTGG 2 cut(s) 278, 1174
BseRI GAGGAG 3 cut(s) 62, 65, 188
BseSI GKGCMC 1 cut(s) 587
BseX3I CGGCCG 1 cut(s) 364
BseXI GCAGC 4 cut(s) 32, 505, 632, 1741
BseYI CCCAGC 2 cut(s) 356, 404
BsgI GTGCAG 1 cut(s) 631
Bsh1236I CGCG 1 cut(s) 172
Bsh1285I CGRYCG 2 cut(s) 367, 741
BshFI GGCC 4 cut(s) 366, 418, 585, 1572
BshTI ACCGGT 2 cut(s) 32, 740
BshVI ATCGAT 2 cut(s) 273, 1460
BsiEI CGRYCG 2 cut(s) 367, 741
BsiHKAI GWGCWC 1 cut(s) 303
BsiHKCI CYCGRG 2 cut(s) 290, 1643
BsiSI CCGG 7 cut(s) 33, 240, 311, 569, 741, 953, 1569
BslI CCNNNNNNNGG 2 cut(s) 295, 310
BsmAI GTCTC 5 cut(s) 74, 125, 185, 1326, 1641
BsmBI CGTCTC 3 cut(s) 74, 125, 185
BsmI GAATGC 1 cut(s) 633
BsnI GGCC 4 cut(s) 366, 418, 585, 1572
Bso31I GGTCTC 1 cut(s) 1326
BsoBI CYCGRG 2 cut(s) 290, 1643
Bsp120I GGGCCC 1 cut(s) 583
Bsp1286I GDGCHC 2 cut(s) 303, 587
Bsp19I CCATGG 2 cut(s) 1017, 1505
BspACI CCGC 5 cut(s) 42, 222, 344, 393, 419
BspANI GGCC 4 cut(s) 366, 418, 585, 1572
BspCNI CTCAG 1 cut(s) 954
BspDI ATCGAT 2 cut(s) 273, 1460
BspFNI CGCG 1 cut(s) 172
BspLI GGNNCC 4 cut(s) 55, 309, 585, 1371
BspMAI CTGCAG 1 cut(s) 1731
BspPI GGATC 7 cut(s) 48, 61, 120, 269, 685, 1029, 1465
BspTI CTTAAG 1 cut(s) 1244
BspTNI GGTCTC 1 cut(s) 1326
BsrFI RCCGGY 2 cut(s) 32, 740
BsrI ACTGG 2 cut(s) 278, 1174
BssAI RCCGGY 2 cut(s) 32, 740
BssECI CCNNGG 4 cut(s) 534, 599, 1017, 1505
BssNAI GTATAC 1 cut(s) 1677
BssNI GRCGYC 1 cut(s) 243
BssT1I CCWWGG 3 cut(s) 534, 1017, 1505
Bst1107I GTATAC 1 cut(s) 1677
Bst2UI CCWGG 4 cut(s) 58, 305, 601, 1455
Bst4CI ACNGT 4 cut(s) 83, 94, 106, 1721
Bst6I CTCTTC 1 cut(s) 188
BstACI GRCGYC 1 cut(s) 243
BstAFI CTTAAG 1 cut(s) 1244
BstBAI YACGTR 1 cut(s) 1181
BstC8I GCNNGC 7 cut(s) 354, 416, 470, 507, 959, 1263, 1603
BstDEI CTNAG 2 cut(s) 962, 1445
BstDSI CCRYGG 2 cut(s) 1017, 1505
BstF5I GGATG 3 cut(s) 556, 1042, 1450
BstFNI CGCG 1 cut(s) 172
BstHHI GCGC 3 cut(s) 174, 575, 1490
BstMAI GTCTC 5 cut(s) 74, 125, 185, 1326, 1641
BstMCI CGRYCG 2 cut(s) 367, 741
BstMWI GCNNNNNNNGC 4 cut(s) 353, 444, 515, 1494
BstNI CCWGG 4 cut(s) 58, 305, 601, 1455
BstNSI RCATGY 2 cut(s) 619, 1083
BstSCI CCNGG 7 cut(s) 56, 303, 309, 568, 599, 952, 1453
BstSFI CTRYAG 1 cut(s) 1727
BstSLI GKGCMC 1 cut(s) 587
BstUI CGCG 1 cut(s) 172
BstV1I GCAGC 4 cut(s) 32, 505, 632, 1741
BstV2I GAAGAC 2 cut(s) 238, 1505
BstX2I RGATCY 3 cut(s) 53, 112, 677
BstYI RGATCY 3 cut(s) 53, 112, 677
BstZ17I GTATAC 1 cut(s) 1677
BstZI CGGCCG 1 cut(s) 364
Bsu15I ATCGAT 2 cut(s) 273, 1460
BsuI GTATCC 1 cut(s) 498
BsuRI GGCC 4 cut(s) 366, 418, 585, 1572
BsuTUI ATCGAT 2 cut(s) 273, 1460
BtgI CCRYGG 2 cut(s) 1017, 1505
BtsCI GGATG 3 cut(s) 556, 1042, 1450
BtsI GCAGTG 1 cut(s) 582
BtsIMutI CAGTG 4 cut(s) 456, 582, 648, 1211
Cac8I GCNNGC 7 cut(s) 354, 416, 470, 507, 959, 1263, 1603
CaiI CAGNNNCTG 1 cut(s) 458
CfoI GCGC 3 cut(s) 174, 575, 1490
Cfr10I RCCGGY 2 cut(s) 32, 740
Cfr13I GGNCC 4 cut(s) 307, 583, 584, 800
ClaI ATCGAT 2 cut(s) 273, 1460
CseI GACGC 1 cut(s) 232
Csp6I GTAC 2 cut(s) 1064, 1467
CspAI ACCGGT 2 cut(s) 32, 740
CviAII CATG 6 cut(s) 616, 1018, 1080, 1506, 1630, 1765
CviQI GTAC 2 cut(s) 1064, 1467
DdeI CTNAG 2 cut(s) 962, 1445
DraI TTTAAA 1 cut(s) 691
EaeI YGGCCR 2 cut(s) 364, 416
EagI CGGCCG 1 cut(s) 364
Eam1104I CTCTTC 1 cut(s) 188
EarI CTCTTC 1 cut(s) 188
EciI GGCGGA 1 cut(s) 382
Ecl136II GAGCTC 1 cut(s) 301
EclXI CGGCCG 1 cut(s) 364
Eco130I CCWWGG 3 cut(s) 534, 1017, 1505
Eco24I GRGCYC 2 cut(s) 303, 587
Eco31I GGTCTC 1 cut(s) 1326
Eco47I GGWCC 2 cut(s) 307, 800
Eco52I CGGCCG 1 cut(s) 364
Eco53kI GAGCTC 1 cut(s) 301
Eco57I CTGAAG 1 cut(s) 1499
Eco88I CYCGRG 2 cut(s) 290, 1643
EcoICRI GAGCTC 1 cut(s) 301
EcoO109I RGGNCCY 1 cut(s) 584
EcoRI GAATTC 1 cut(s) 1007
EcoRII CCWGG 4 cut(s) 56, 303, 599, 1453
EcoT14I CCWWGG 3 cut(s) 534, 1017, 1505
EcoT22I ATGCAT 1 cut(s) 1161
EcoT38I GRGCYC 2 cut(s) 303, 587
ErhI CCWWGG 3 cut(s) 534, 1017, 1505
Esp3I CGTCTC 3 cut(s) 74, 125, 185
FaeI CATG 6 cut(s) 619, 1021, 1083, 1509, 1633, 1768
FatI CATG 6 cut(s) 615, 1017, 1079, 1505, 1629, 1764
FauNDI CATATG 1 cut(s) 658
FblI GTMKAC 1 cut(s) 1676
Fnu4HI GCNGC 8 cut(s) 43, 46, 222, 345, 419, 519, 646, 1730
FokI GGATG 3 cut(s) 563, 1049, 1437
FriOI GRGCYC 2 cut(s) 303, 587
Fsp4HI GCNGC 8 cut(s) 43, 46, 222, 345, 419, 519, 646, 1730
FspBI CTAG 3 cut(s) 143, 612, 1067
FspI TGCGCA 1 cut(s) 1489
GlaI GCGC 3 cut(s) 173, 574, 1489
GluI GCNGC 8 cut(s) 43, 46, 222, 345, 419, 519, 646, 1730
GsaI CCCAGC 2 cut(s) 360, 408
HaeIII GGCC 4 cut(s) 366, 418, 585, 1572
HapII CCGG 7 cut(s) 33, 240, 311, 569, 741, 953, 1569
HgaI GACGC 1 cut(s) 232
HhaI GCGC 3 cut(s) 174, 575, 1490
Hin1I GRCGYC 1 cut(s) 243
Hin1II CATG 6 cut(s) 619, 1021, 1083, 1509, 1633, 1768
Hin6I GCGC 3 cut(s) 172, 573, 1488
HinP1I GCGC 3 cut(s) 172, 573, 1488
HindIII AAGCTT 1 cut(s) 1495
HinfI GANTC 6 cut(s) 167, 565, 1057, 1480, 1761, 1784
HpaII CCGG 7 cut(s) 33, 240, 311, 569, 741, 953, 1569
HphI GGTGA 3 cut(s) 26, 140, 791
Hpy166II GTNNAC 6 cut(s) 97, 283, 803, 920, 1677, 1724
Hpy188I TCNGA 3 cut(s) 53, 339, 1659
Hpy188III TCNNGA 6 cut(s) 143, 187, 638, 863, 1379, 1645
Hpy8I GTNNAC 6 cut(s) 97, 283, 803, 920, 1677, 1724
HpyAV CCTTC 5 cut(s) 289, 469, 535, 638, 1665
HpyCH4III ACNGT 4 cut(s) 83, 94, 106, 1721
HpyCH4IV ACGT 3 cut(s) 380, 772, 1180
HpyF10VI GCNNNNNNNGC 4 cut(s) 353, 444, 515, 1494
HpyF3I CTNAG 2 cut(s) 962, 1445
HpySE526I ACGT 3 cut(s) 380, 772, 1180
Hsp92I GRCGYC 1 cut(s) 243
Hsp92II CATG 6 cut(s) 619, 1021, 1083, 1509, 1633, 1768
HspAI GCGC 3 cut(s) 172, 573, 1488
LmnI GCTCC 6 cut(s) 242, 298, 306, 630, 977, 1571
Lsp1109I GCAGC 4 cut(s) 32, 505, 632, 1741
LweI GCATC 5 cut(s) 57, 148, 276, 1027, 1741
MaeI CTAG 3 cut(s) 143, 612, 1067
MaeII ACGT 3 cut(s) 380, 772, 1180
MaeIII GTNAC 5 cut(s) 28, 779, 1338, 1410, 1523
MboII GAAGA 5 cut(s) 175, 238, 994, 1505, 1680
MfeI CAATTG 1 cut(s) 1347
MflI RGATCY 3 cut(s) 53, 112, 677
MhlI GDGCHC 2 cut(s) 303, 587
MluCI AATT 9 cut(s) 527, 607, 825, 868, 1007, 1026, 1231, 1347, 1615
MlyI GAGTC 3 cut(s) 176, 1051, 1474
MmeI TCCRAC 1 cut(s) 1267
Mph1103I ATGCAT 1 cut(s) 1161
MslI CAYNNNNRTG 3 cut(s) 620, 716, 1016
MspCI CTTAAG 1 cut(s) 1244
MspI CCGG 7 cut(s) 33, 240, 311, 569, 741, 953, 1569
MspR9I CCNGG 7 cut(s) 58, 305, 311, 570, 601, 954, 1455
MunI CAATTG 1 cut(s) 1347
Mva1269I GAATGC 1 cut(s) 633
MvaI CCWGG 4 cut(s) 58, 305, 601, 1455
MvnI CGCG 1 cut(s) 172
MwoI GCNNNNNNNGC 4 cut(s) 353, 444, 515, 1494
NciI CCSGG 3 cut(s) 311, 570, 954
NcoI CCATGG 2 cut(s) 1017, 1505
NdeI CATATG 1 cut(s) 658
NlaIII CATG 6 cut(s) 619, 1021, 1083, 1509, 1633, 1768
NlaIV GGNNCC 4 cut(s) 55, 309, 585, 1371
NmuCI GTSAC 1 cut(s) 779
NsbI TGCGCA 1 cut(s) 1489
NsiI ATGCAT 1 cut(s) 1161
NspI RCATGY 2 cut(s) 619, 1083
PciI ACATGT 1 cut(s) 1079
PcsI WCGNNNNNNNCGW 1 cut(s) 198
PctI GAATGC 1 cut(s) 633
PfeI GAWTC 3 cut(s) 565, 1761, 1784
PfoI TCCNGGA 2 cut(s) 303, 1453
PinAI ACCGGT 2 cut(s) 32, 740
PkrI GCNGC 8 cut(s) 44, 47, 223, 346, 420, 520, 647, 1731
PleI GAGTC 3 cut(s) 175, 1051, 1474
PpsI GAGTC 3 cut(s) 175, 1051, 1474
Ppu21I YACGTR 1 cut(s) 1181
PscI ACATGT 1 cut(s) 1079
PshBI ATTAAT 1 cut(s) 1224
Psp124BI GAGCTC 1 cut(s) 303
Psp6I CCWGG 4 cut(s) 56, 303, 599, 1453
PspFI CCCAGC 2 cut(s) 356, 404
PspGI CCWGG 4 cut(s) 56, 303, 599, 1453
PspN4I GGNNCC 4 cut(s) 55, 309, 585, 1371
PspOMI GGGCCC 1 cut(s) 583
PspPI GGNCC 4 cut(s) 307, 583, 584, 800
PstI CTGCAG 1 cut(s) 1731
PstNI CAGNNNCTG 1 cut(s) 458
PsuI RGATCY 3 cut(s) 53, 112, 677
RsaI GTAC 2 cut(s) 1065, 1468
RsaNI GTAC 2 cut(s) 1064, 1467
RseI CAYNNNNRTG 3 cut(s) 620, 716, 1016
SacI GAGCTC 1 cut(s) 303
SatI GCNGC 8 cut(s) 43, 46, 222, 345, 419, 519, 646, 1730
Sau96I GGNCC 4 cut(s) 307, 583, 584, 800
SchI GAGTC 3 cut(s) 176, 1051, 1474
ScrFI CCNGG 7 cut(s) 58, 305, 311, 570, 601, 954, 1455
SduI GDGCHC 2 cut(s) 303, 587
SfaNI GCATC 5 cut(s) 57, 148, 276, 1027, 1741
SfcI CTRYAG 1 cut(s) 1727
SinI GGWCC 2 cut(s) 307, 800
SmiMI CAYNNNNRTG 3 cut(s) 620, 716, 1016
SmlI CTYRAG 3 cut(s) 116, 638, 1244
SmoI CTYRAG 3 cut(s) 116, 638, 1244
SpeI ACTAGT 1 cut(s) 1066
Sse9I AATT 9 cut(s) 527, 607, 825, 868, 1007, 1026, 1231, 1347, 1615
SsiI CCGC 5 cut(s) 42, 222, 344, 393, 419
SspI AATATT 1 cut(s) 1111
SspMI CTAG 3 cut(s) 143, 612, 1067
SstI GAGCTC 1 cut(s) 303
StyD4I CCNGG 7 cut(s) 56, 303, 309, 568, 599, 952, 1453
StyI CCWWGG 3 cut(s) 534, 1017, 1505
TaaI ACNGT 4 cut(s) 83, 94, 106, 1721
TaiI ACGT 3 cut(s) 383, 775, 1183
TaqI TCGA 9 cut(s) 5, 192, 201, 273, 386, 497, 906, 1268, 1460
TaqII GACCGA 1 cut(s) 788
TasI AATT 9 cut(s) 527, 607, 825, 868, 1007, 1026, 1231, 1347, 1615
TatI WGTACW 2 cut(s) 1063, 1466
TauI GCSGC 4 cut(s) 45, 224, 347, 421
TfiI GAWTC 3 cut(s) 565, 1761, 1784
TscAI CASTG 4 cut(s) 463, 582, 655, 1211
TseFI GTSAC 1 cut(s) 779
TseI GCWGC 4 cut(s) 45, 518, 645, 1729
Tsp45I GTSAC 1 cut(s) 779
TspDTI ATGAA 6 cut(s) 837, 1000, 1007, 1241, 1646, 1760
TspGWI ACGGA 3 cut(s) 56, 167, 990
TspRI CASTG 4 cut(s) 463, 582, 655, 1211
Vha464I CTTAAG 1 cut(s) 1244
VpaK11BI GGWCC 2 cut(s) 307, 800
VspI ATTAAT 1 cut(s) 1224
XapI RAATTY 2 cut(s) 527, 1007
XbaI TCTAGA 1 cut(s) 142
XceI RCATGY 2 cut(s) 619, 1083
XmiI GTMKAC 1 cut(s) 1676
XspI CTAG 3 cut(s) 143, 612, 1067
Zsp2I ATGCAT 1 cut(s) 1161
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.