pycom11g05470

cinnamoyl-CoA reductase 1-like

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
pyrus_communis
Chr11
OG0003662
Physical Location & Seq
Forward (+)
4162460 .. 4164608
2149 bp
Loading structure...
UTR
Exon/CDS
Intron
N/A

Sequence Viewer

Length: 951 bp
ATGCGTTACCGGTGGAAGCGGCTGCATCGGATCCATCGCTCGTCCGTCTCCTCCTCCACCGTGATTACACCGTCCACGCCACCGTCAAGGATCTCAGACGAGGGCGAGACGAAGCATCTAGAAGCGTTAGTAGAGGGGGCAGAGTCGCGCCTCCGTCTCTTCCAGATCGACCTCCTCGACTACAACTCCATACTCGCCGCCGTCGATGGTTGCTCCGGCGTCTTCCACCTCGCCTCTCCCTGCATCGTCGATCAAGTCCACGACCCCGAGAAGGAGCTCCTGGACCCGGCGATCAAAGGAACGCTCAATGTTCTGACGGCGGCGAAGCAGGCTGGGGTCAGCCGTGTGGTGCTGACGTCATCCATCTCCGCCATCACTCCCAGCCCAAGCTGGCCGTCCGATAAGATCAAGGGCGAGGATTGCTGGACAGACATTGACTACTGCAAGCAGAAGGGATTGTGGTATCCATTATCGAAAACGCTGGCGGAGAAAGCTGCGTGGGAATTTGCCAAGGAGAAGGGGCTGGATGTGGTTGTGGTGAATCCGGGCACGGTGATGGGCCCTGTTATCTCGCCCAGGCTCAATGCTAGCATGTTGATGCTCCTTCGCATTCTTGAGGGCTGCACTGAAACATATGAGGAATTCTTTATGGGATCTGTACATTTTAAAGATGTTGCTCTCGCACACATTTTAGTGTATGAGAACAAATCAGCGACCGGTAGGCACTTGTGTTTGGAAGCTATATCACGTTATGGTGACTTTGTGGCAAAGGTTGCTGAACTTTACCCTGAGTACAAGGTTCGCAGTTTGCCAAAGGACACCCAACCGGGCTTGCTGAGGGAAAAGAATGGAGCAAAGAAGCTAATGAACATGGGTTTAGAATTCATTCCCATGGATCAAATTATCAAGGATGGTGTTGAGAGCCTAAAGAGCAAGGGATTTATTTCATAA
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
No pathways identified.
Pfam Domains
Protein Families
Epimerase

Protein Analysis

317

Amino Acids

35.07

Weight (kDa)

7.08

Isoelectric Point (pI)

34.37

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
GDP_Man_Dehyd PF16363 34 - 121 1e-05 GDP-mannose 4,6 dehydratase
Epimerase PF01370 43 - 238 5.6e-16 NAD dependent epimerase/dehydratase family
NAD_binding_10 PF13460 47 - 184 4.8e-08 NAD(P)H-binding
3Beta_HSD PF01073 51 - 232 4.6e-14 3-beta hydroxysteroid dehydrogenase/isomerase family
NAD_binding_4 PF07993 68 - 193 1.3e-07 Male sterility protein
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Publication-ready
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Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AatII GACGTC 1 cut(s) 359
AccII CGCG 1 cut(s) 148
AciI CCGC 5 cut(s) 19, 198, 320, 369, 485
AclWI GGATC 5 cut(s) 25, 38, 98, 661, 903
AcoI YGGCCR 1 cut(s) 392
AcsI RAATTY 3 cut(s) 503, 641, 881
AcyI GRCGYC 2 cut(s) 219, 356
AfaI GTAC 2 cut(s) 660, 794
AfiI CCNNNNNNNGG 2 cut(s) 271, 286
AgeI ACCGGT 2 cut(s) 9, 716
AjnI CCWGG 2 cut(s) 279, 575
AluBI AGCT 5 cut(s) 277, 390, 494, 740, 862
AluI AGCT 5 cut(s) 277, 390, 494, 740, 862
Alw21I GWGCWC 1 cut(s) 279
Alw26I GTCTC 3 cut(s) 52, 101, 161
AlwI GGATC 5 cut(s) 25, 38, 98, 661, 903
Ama87I CYCGRG 1 cut(s) 266
AoxI GGCC 2 cut(s) 392, 559
ApaI GGGCCC 1 cut(s) 563
ApeKI GCWGC 3 cut(s) 22, 494, 621
ApoI RAATTY 3 cut(s) 503, 641, 881
AsiGI ACCGGT 2 cut(s) 9, 716
Asp700I GAANNNNTTC 1 cut(s) 885
AspLEI GCGC 1 cut(s) 150
AspS9I GGNCC 3 cut(s) 283, 559, 560
AsuC2I CCSGG 3 cut(s) 287, 546, 828
AsuHPI GGTGA 3 cut(s) 550, 565, 767
AsuNHI GCTAGC 1 cut(s) 587
AvaI CYCGRG 1 cut(s) 266
AvaII GGWCC 1 cut(s) 283
BaeGI GKGCMC 2 cut(s) 551, 563
BamHI GGATCC 1 cut(s) 30
BanII GRGCYC 2 cut(s) 279, 563
BbsI GAAGAC 1 cut(s) 214
Bbv12I GWGCWC 1 cut(s) 279
BbvCI CCTCAGC 1 cut(s) 836
BbvI GCAGC 3 cut(s) 9, 481, 608
BccI CCATC 6 cut(s) 42, 200, 371, 380, 550, 905
BceAI ACGGC 4 cut(s) 185, 327, 333, 379
BciT130I CCWGG 2 cut(s) 281, 577
BciVI GTATCC 1 cut(s) 474
BcnI CCSGG 3 cut(s) 287, 546, 828
BcoDI GTCTC 3 cut(s) 52, 101, 161
BfaI CTAG 2 cut(s) 119, 588
BfuI GTATCC 1 cut(s) 474
BisI GCNGC 6 cut(s) 20, 23, 198, 321, 495, 622
BlsI GCNGC 6 cut(s) 21, 24, 199, 322, 496, 623
Bme1390I CCNGG 5 cut(s) 281, 287, 546, 577, 828
Bme18I GGWCC 1 cut(s) 283
BmeT110I CYCGRG 1 cut(s) 266
BmgT120I GGNCC 3 cut(s) 283, 559, 560
BmiI GGNNCC 3 cut(s) 32, 285, 561
BmrFI CCNGG 5 cut(s) 281, 287, 546, 577, 828
BmsI GCATC 4 cut(s) 34, 124, 252, 588
BmtI GCTAGC 1 cut(s) 591
BpiI GAAGAC 1 cut(s) 214
Bpu10I CCTNAGC 1 cut(s) 836
BpuEI CTTGAG 1 cut(s) 635
BpuMI CCSGG 3 cut(s) 287, 546, 828
BsaHI GRCGYC 2 cut(s) 219, 356
BsaJI CCNNGG 3 cut(s) 510, 575, 891
BsaWI WCCGGW 2 cut(s) 9, 716
BsaXI ACNNNNNCTCC 2 cut(s) 170, 200
Bsc4I CCNNNNNNNGG 2 cut(s) 271, 286
Bse118I RCCGGY 2 cut(s) 9, 716
BseBI CCWGG 2 cut(s) 281, 577
BseDI CCNNGG 3 cut(s) 510, 575, 891
BseGI GGATG 3 cut(s) 359, 532, 916
BseLI CCNNNNNNNGG 2 cut(s) 271, 286
BseMII CTCAG 3 cut(s) 108, 780, 827
BseRI GAGGAG 3 cut(s) 40, 43, 164
BseSI GKGCMC 2 cut(s) 551, 563
BseXI GCAGC 3 cut(s) 9, 481, 608
BseYI CCCAGC 2 cut(s) 332, 380
BsgI GTGCAG 1 cut(s) 607
Bsh1236I CGCG 1 cut(s) 148
Bsh1285I CGRYCG 1 cut(s) 717
BshFI GGCC 2 cut(s) 394, 561
BshTI ACCGGT 2 cut(s) 9, 716
BsiEI CGRYCG 1 cut(s) 717
BsiHKAI GWGCWC 1 cut(s) 279
BsiHKCI CYCGRG 1 cut(s) 266
BsiSI CCGG 6 cut(s) 10, 216, 287, 545, 717, 827
BslI CCNNNNNNNGG 2 cut(s) 271, 286
BsmAI GTCTC 3 cut(s) 52, 101, 161
BsmBI CGTCTC 3 cut(s) 52, 101, 161
BsmI GAATGC 1 cut(s) 609
BsnI GGCC 2 cut(s) 394, 561
BsoBI CYCGRG 1 cut(s) 266
Bsp120I GGGCCC 1 cut(s) 559
Bsp1286I GDGCHC 3 cut(s) 279, 551, 563
Bsp1407I TGTACA 1 cut(s) 658
Bsp143I GATC 8 cut(s) 30, 90, 165, 250, 291, 405, 653, 895
Bsp19I CCATGG 1 cut(s) 891
BspACI CCGC 5 cut(s) 19, 198, 320, 369, 485
BspANI GGCC 2 cut(s) 394, 561
BspCNI CTCAG 3 cut(s) 107, 781, 828
BspFNI CGCG 1 cut(s) 148
BspLI GGNNCC 3 cut(s) 32, 285, 561
BspOI GCTAGC 1 cut(s) 591
BspPI GGATC 5 cut(s) 25, 38, 98, 661, 903
BsrFI RCCGGY 2 cut(s) 9, 716
BsrGI TGTACA 1 cut(s) 658
BssAI RCCGGY 2 cut(s) 9, 716
BssECI CCNNGG 3 cut(s) 510, 575, 891
BssMI GATC 8 cut(s) 30, 90, 165, 250, 291, 405, 653, 895
BssNI GRCGYC 2 cut(s) 219, 356
BssT1I CCWWGG 2 cut(s) 510, 891
Bst2UI CCWGG 2 cut(s) 281, 577
Bst4CI ACNGT 4 cut(s) 61, 72, 84, 553
Bst6I CTCTTC 1 cut(s) 164
BstACI GRCGYC 2 cut(s) 219, 356
BstAPI GCANNNNNTGC 1 cut(s) 773
BstAUI TGTACA 1 cut(s) 658
BstC8I GCNNGC 6 cut(s) 330, 392, 446, 483, 589, 833
BstDEI CTNAG 3 cut(s) 94, 789, 836
BstDSI CCRYGG 1 cut(s) 891
BstF5I GGATG 3 cut(s) 359, 532, 916
BstFNI CGCG 1 cut(s) 148
BstHHI GCGC 1 cut(s) 150
BstKTI GATC 8 cut(s) 33, 93, 168, 253, 294, 408, 656, 898
BstMAI GTCTC 3 cut(s) 52, 101, 161
BstMBI GATC 8 cut(s) 30, 90, 165, 250, 291, 405, 653, 895
BstMCI CGRYCG 1 cut(s) 717
BstMWI GCNNNNNNNGC 5 cut(s) 329, 420, 491, 773, 930
BstNI CCWGG 2 cut(s) 281, 577
BstNSI RCATGY 1 cut(s) 595
BstSCI CCNGG 5 cut(s) 279, 285, 544, 575, 826
BstSLI GKGCMC 2 cut(s) 551, 563
BstUI CGCG 1 cut(s) 148
BstV1I GCAGC 3 cut(s) 9, 481, 608
BstV2I GAAGAC 1 cut(s) 214
BstX2I RGATCY 3 cut(s) 30, 90, 653
BstYI RGATCY 3 cut(s) 30, 90, 653
BsuI GTATCC 1 cut(s) 474
BsuRI GGCC 2 cut(s) 394, 561
BtgI CCRYGG 1 cut(s) 891
BtgZI GCGATG 1 cut(s) 20
BtsCI GGATG 3 cut(s) 359, 532, 916
BtsIMutI CAGTG 1 cut(s) 624
Cac8I GCNNGC 6 cut(s) 330, 392, 446, 483, 589, 833
CfoI GCGC 1 cut(s) 150
Cfr10I RCCGGY 2 cut(s) 9, 716
Cfr13I GGNCC 3 cut(s) 283, 559, 560
CseI GACGC 1 cut(s) 208
Csp6I GTAC 2 cut(s) 659, 793
CspAI ACCGGT 2 cut(s) 9, 716
CviAII CATG 3 cut(s) 592, 871, 892
CviQI GTAC 2 cut(s) 659, 793
DdeI CTNAG 3 cut(s) 94, 789, 836
DpnI GATC 8 cut(s) 32, 92, 167, 252, 293, 407, 655, 897
DpnII GATC 8 cut(s) 30, 90, 165, 250, 291, 405, 653, 895
DraI TTTAAA 1 cut(s) 667
EaeI YGGCCR 1 cut(s) 392
Eam1104I CTCTTC 1 cut(s) 164
EarI CTCTTC 1 cut(s) 164
EciI GGCGGA 2 cut(s) 358, 500
Ecl136II GAGCTC 1 cut(s) 277
Eco130I CCWWGG 2 cut(s) 510, 891
Eco24I GRGCYC 2 cut(s) 279, 563
Eco47I GGWCC 1 cut(s) 283
Eco53kI GAGCTC 1 cut(s) 277
Eco88I CYCGRG 1 cut(s) 266
EcoICRI GAGCTC 1 cut(s) 277
EcoO109I RGGNCCY 1 cut(s) 560
EcoRI GAATTC 2 cut(s) 641, 881
EcoRII CCWGG 2 cut(s) 279, 575
EcoT14I CCWWGG 2 cut(s) 510, 891
EcoT38I GRGCYC 2 cut(s) 279, 563
ErhI CCWWGG 2 cut(s) 510, 891
Esp3I CGTCTC 3 cut(s) 52, 101, 161
FaeI CATG 3 cut(s) 595, 874, 895
FatI CATG 3 cut(s) 591, 870, 891
FauNDI CATATG 1 cut(s) 634
Fnu4HI GCNGC 6 cut(s) 20, 23, 198, 321, 495, 622
FokI GGATG 3 cut(s) 346, 539, 923
FriOI GRGCYC 2 cut(s) 279, 563
Fsp4HI GCNGC 6 cut(s) 20, 23, 198, 321, 495, 622
FspBI CTAG 2 cut(s) 119, 588
GlaI GCGC 1 cut(s) 149
GluI GCNGC 6 cut(s) 20, 23, 198, 321, 495, 622
GsaI CCCAGC 2 cut(s) 336, 384
HaeIII GGCC 2 cut(s) 394, 561
HapII CCGG 6 cut(s) 10, 216, 287, 545, 717, 827
HgaI GACGC 1 cut(s) 208
HhaI GCGC 1 cut(s) 150
Hin1I GRCGYC 2 cut(s) 219, 356
Hin1II CATG 3 cut(s) 595, 874, 895
Hin6I GCGC 1 cut(s) 148
HinP1I GCGC 1 cut(s) 148
HinfI GANTC 2 cut(s) 143, 541
HpaII CCGG 6 cut(s) 10, 216, 287, 545, 717, 827
HphI GGTGA 3 cut(s) 550, 565, 767
Hpy166II GTNNAC 2 cut(s) 75, 259
Hpy188I TCNGA 4 cut(s) 30, 97, 315, 400
Hpy188III TCNNGA 3 cut(s) 119, 163, 614
Hpy8I GTNNAC 2 cut(s) 75, 259
Hpy99I CGWCG 2 cut(s) 206, 251
HpyAV CCTTC 4 cut(s) 265, 445, 511, 614
HpyCH4III ACNGT 4 cut(s) 61, 72, 84, 553
HpyCH4IV ACGT 2 cut(s) 356, 748
HpyCH4V TGCA 4 cut(s) 25, 243, 444, 624
HpyF10VI GCNNNNNNNGC 5 cut(s) 329, 420, 491, 773, 930
HpyF3I CTNAG 3 cut(s) 94, 789, 836
HpySE526I ACGT 2 cut(s) 356, 748
Hsp92I GRCGYC 2 cut(s) 219, 356
Hsp92II CATG 3 cut(s) 595, 874, 895
HspAI GCGC 1 cut(s) 148
Kzo9I GATC 8 cut(s) 30, 90, 165, 250, 291, 405, 653, 895
LmnI GCTCC 5 cut(s) 218, 274, 282, 606, 851
Lsp1109I GCAGC 3 cut(s) 9, 481, 608
LweI GCATC 4 cut(s) 34, 124, 252, 588
MaeI CTAG 2 cut(s) 119, 588
MaeII ACGT 2 cut(s) 356, 748
MaeIII GTNAC 2 cut(s) 5, 755
MalI GATC 8 cut(s) 32, 92, 167, 252, 293, 407, 655, 897
MboI GATC 8 cut(s) 30, 90, 165, 250, 291, 405, 653, 895
MboII GAAGA 2 cut(s) 151, 214
MflI RGATCY 3 cut(s) 30, 90, 653
MhlI GDGCHC 3 cut(s) 279, 551, 563
MluCI AATT 4 cut(s) 503, 641, 881, 900
MlyI GAGTC 1 cut(s) 152
MroXI GAANNNNTTC 1 cut(s) 885
MseI TTAA 1 cut(s) 666
MslI CAYNNNNRTG 4 cut(s) 554, 596, 692, 890
MspI CCGG 6 cut(s) 10, 216, 287, 545, 717, 827
MspR9I CCNGG 5 cut(s) 281, 287, 546, 577, 828
Mva1269I GAATGC 1 cut(s) 609
MvaI CCWGG 2 cut(s) 281, 577
MvnI CGCG 1 cut(s) 148
MwoI GCNNNNNNNGC 5 cut(s) 329, 420, 491, 773, 930
NciI CCSGG 3 cut(s) 287, 546, 828
NcoI CCATGG 1 cut(s) 891
NdeI CATATG 1 cut(s) 634
NdeII GATC 8 cut(s) 30, 90, 165, 250, 291, 405, 653, 895
NheI GCTAGC 1 cut(s) 587
NlaIII CATG 3 cut(s) 595, 874, 895
NlaIV GGNNCC 3 cut(s) 32, 285, 561
NmuCI GTSAC 1 cut(s) 755
NspI RCATGY 1 cut(s) 595
PcsI WCGNNNNNNNCGW 2 cut(s) 174, 201
PctI GAATGC 1 cut(s) 609
PdmI GAANNNNTTC 1 cut(s) 885
PfeI GAWTC 1 cut(s) 541
PfoI TCCNGGA 1 cut(s) 279
PinAI ACCGGT 2 cut(s) 9, 716
PkrI GCNGC 6 cut(s) 21, 24, 199, 322, 496, 623
PleI GAGTC 1 cut(s) 151
PpsI GAGTC 1 cut(s) 151
Psp124BI GAGCTC 1 cut(s) 279
Psp6I CCWGG 2 cut(s) 279, 575
PspFI CCCAGC 2 cut(s) 332, 380
PspGI CCWGG 2 cut(s) 279, 575
PspN4I GGNNCC 3 cut(s) 32, 285, 561
PspOMI GGGCCC 1 cut(s) 559
PspPI GGNCC 3 cut(s) 283, 559, 560
PsuI RGATCY 3 cut(s) 30, 90, 653
RsaI GTAC 2 cut(s) 660, 794
RsaNI GTAC 2 cut(s) 659, 793
RseI CAYNNNNRTG 4 cut(s) 554, 596, 692, 890
SacI GAGCTC 1 cut(s) 279
SaqAI TTAA 1 cut(s) 666
SatI GCNGC 6 cut(s) 20, 23, 198, 321, 495, 622
Sau3AI GATC 8 cut(s) 30, 90, 165, 250, 291, 405, 653, 895
Sau96I GGNCC 3 cut(s) 283, 559, 560
SchI GAGTC 1 cut(s) 152
ScrFI CCNGG 5 cut(s) 281, 287, 546, 577, 828
SduI GDGCHC 3 cut(s) 279, 551, 563
SfaNI GCATC 4 cut(s) 34, 124, 252, 588
SinI GGWCC 1 cut(s) 283
SmiMI CAYNNNNRTG 4 cut(s) 554, 596, 692, 890
SmlI CTYRAG 1 cut(s) 614
SmoI CTYRAG 1 cut(s) 614
Sse9I AATT 4 cut(s) 503, 641, 881, 900
SsiI CCGC 5 cut(s) 19, 198, 320, 369, 485
SspMI CTAG 2 cut(s) 119, 588
SstI GAGCTC 1 cut(s) 279
StyD4I CCNGG 5 cut(s) 279, 285, 544, 575, 826
StyI CCWWGG 2 cut(s) 510, 891
TaaI ACNGT 4 cut(s) 61, 72, 84, 553
TaiI ACGT 2 cut(s) 359, 751
TaqI TCGA 5 cut(s) 168, 177, 204, 249, 473
TasI AATT 4 cut(s) 503, 641, 881, 900
TatI WGTACW 2 cut(s) 658, 792
TauI GCSGC 3 cut(s) 22, 200, 323
TfiI GAWTC 1 cut(s) 541
Tru1I TTAA 1 cut(s) 666
Tru9I TTAA 1 cut(s) 666
TscAI CASTG 1 cut(s) 631
TseFI GTSAC 1 cut(s) 755
TseI GCWGC 3 cut(s) 22, 494, 621
Tsp45I GTSAC 1 cut(s) 755
TspDTI ATGAA 3 cut(s) 874, 881, 936
TspGWI ACGGA 2 cut(s) 34, 143
TspRI CASTG 1 cut(s) 631
VpaK11BI GGWCC 1 cut(s) 283
XapI RAATTY 3 cut(s) 503, 641, 881
XbaI TCTAGA 1 cut(s) 118
XceI RCATGY 1 cut(s) 595
XmnI GAANNNNTTC 1 cut(s) 885
XspI CTAG 2 cut(s) 119, 588
ZraI GACGTC 1 cut(s) 357
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.