pycom11g11620

Bifunctional monodehydroascorbate reductase and carbonic anhydrase nectarin-3-like

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
pyrus_communis
Chr11
OG0006048
Physical Location & Seq
Reverse (-)
10312580 .. 10314481
1902 bp
Loading structure...
UTR
Exon/CDS
Intron
N/A

Sequence Viewer

Length: 828 bp
ATGGAGAAGTTTCCCACCCCAATATTATTTTACAGTTTCCTTATTGCCTTGGTATTGCTGAATTCTCGCCATGCAACATCTGAAGAAGTTGAAGATCAGAGGGAGTTTAATTATGAGGAAGACAGTGAGAAGGGACCGCTTCGATGGGGAGAGATACGCCAAGAATGGAGCATGTGCAGCAAGGGATCGATGCAGTCTCCGATTGATCTGTCAGATGACAGAGTCGAACTAGTGTCACATTTGGGGATACTTAAGAGTAACTACAGGCCTTGCAATGCCACTCTTAAGAATAGGGGACATGATATGATGTTGAAATGGGAAACTGATGCAGGATTTATACTATTAAACGGAACTTTGTATAAACTGAAACAATGCCACTGGCACGCGTCTTCAGAACACACCATCAATGGAAAGAGGTTTGATTTAGAGGTGCATTTGGTTCATGAGAGCGCAACTGGAAATATTGCTGTGACTGGAATTCTTTATAGGATTGGAGCTCCTGATCCATTTTTGTCATCGATCATGGATTATTTAGCAGACGTCTCTGATACCACTGACGAGGAAGAAGTGGTTGGTATGGTTGATCCAAGGCAAATGAAAACAAGCAGCACAAAGTATTACAGATATATTGGCTCACTTACAACTCCCCCTTGCACACAAAATGTTATCTGGACCATTGCTCGAGAGGTGAGCACTGTCTCAGAAGAACAAGTGAAATCACTCCGAGTAGCTGTTCATGATGACTCAGACACTAATGCAAGACCAGTACAACCCAGAAACAGGCGGATAGTGCAACTTTATAGACAAACAGTAGAGGAAAAGAATTGA
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
GO:0005575 GO:0008150 GO:0010033 GO:0010037 GO:0016020 GO:0042221 GO:0042493 GO:0050896 GO:0072347 GO:1901700
KEGG Pathways
Metabolic & Signaling
ko00910 map00910
Pfam Domains
Protein Families
Carb_anhydrase

Protein Analysis

276

Amino Acids

31.53

Weight (kDa)

5.52

Isoelectric Point (pI)

39.85

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
Carb_anhydrase PF00194 45 - 265 5.1e-44 Eukaryotic-type carbonic anhydrase
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

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Publication-ready
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Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AatII GACGTC 1 cut(s) 543
AccII CGCG 1 cut(s) 386
AciI CCGC 2 cut(s) 137, 784
AclWI GGATC 3 cut(s) 193, 497, 578
AcsI RAATTY 2 cut(s) 61, 477
AcuI CTGAAG 2 cut(s) 102, 375
AcyI GRCGYC 1 cut(s) 540
AfaI GTAC 1 cut(s) 768
AfiI CCNNNNNNNGG 1 cut(s) 780
AflII CTTAAG 2 cut(s) 251, 284
AflIII ACRYGT 1 cut(s) 384
AgsI TTSAA 2 cut(s) 92, 313
AhlI ACTAGT 1 cut(s) 229
AjuI GAANNNNNNNTTGG 2 cut(s) 555, 587
AluBI AGCT 2 cut(s) 497, 731
AluI AGCT 2 cut(s) 497, 731
Alw21I GWGCWC 2 cut(s) 499, 695
Alw26I GTCTC 3 cut(s) 201, 547, 703
AlwI GGATC 3 cut(s) 193, 497, 578
Ama87I CYCGRG 1 cut(s) 681
AoxI GGCC 1 cut(s) 266
ApeKI GCWGC 2 cut(s) 177, 606
ApoI RAATTY 2 cut(s) 61, 477
AspLEI GCGC 1 cut(s) 452
AspS9I GGNCC 2 cut(s) 134, 672
AsuHPI GGTGA 1 cut(s) 700
AvaI CYCGRG 1 cut(s) 681
AvaII GGWCC 2 cut(s) 134, 672
BanII GRGCYC 1 cut(s) 499
BbsI GAAGAC 2 cut(s) 126, 381
Bbv12I GWGCWC 2 cut(s) 499, 695
BbvI GCAGC 2 cut(s) 189, 618
BccI CCATC 2 cut(s) 138, 410
BciVI GTATCC 1 cut(s) 240
BcoDI GTCTC 3 cut(s) 201, 547, 703
BcuI ACTAGT 1 cut(s) 229
BfaI CTAG 1 cut(s) 230
BfmI CTRYAG 1 cut(s) 262
BfrI CTTAAG 2 cut(s) 251, 284
BfuI GTATCC 1 cut(s) 240
BisI GCNGC 2 cut(s) 178, 607
BlsI GCNGC 2 cut(s) 179, 608
Bme18I GGWCC 2 cut(s) 134, 672
BmeT110I CYCGRG 1 cut(s) 681
BmgT120I GGNCC 2 cut(s) 134, 672
BmiI GGNNCC 1 cut(s) 135
BmsI GCATC 2 cut(s) 180, 316
BpiI GAAGAC 2 cut(s) 126, 381
Bsa29I ATCGAT 2 cut(s) 188, 518
BsaHI GRCGYC 1 cut(s) 540
BsaJI CCNNGG 2 cut(s) 48, 587
Bsc4I CCNNNNNNNGG 1 cut(s) 780
Bse1I ACTGG 4 cut(s) 383, 460, 478, 764
Bse3DI GCAATG 2 cut(s) 280, 675
BseCI ATCGAT 2 cut(s) 188, 518
BseDI CCNNGG 2 cut(s) 48, 587
BseLI CCNNNNNNNGG 1 cut(s) 780
BseMI GCAATG 2 cut(s) 280, 675
BseMII CTCAG 2 cut(s) 714, 759
BseNI ACTGG 4 cut(s) 383, 460, 478, 764
BseXI GCAGC 2 cut(s) 189, 618
BsgI GTGCAG 1 cut(s) 196
Bsh1236I CGCG 1 cut(s) 386
BshFI GGCC 1 cut(s) 268
BshVI ATCGAT 2 cut(s) 188, 518
BsiHKAI GWGCWC 2 cut(s) 499, 695
BsiHKCI CYCGRG 1 cut(s) 681
BslFI GGGAC 2 cut(s) 147, 309
BslI CCNNNNNNNGG 1 cut(s) 780
BsmAI GTCTC 3 cut(s) 201, 547, 703
BsmBI CGTCTC 1 cut(s) 547
BsmFI GGGAC 2 cut(s) 147, 309
BsnI GGCC 1 cut(s) 268
BsoBI CYCGRG 1 cut(s) 681
Bsp1286I GDGCHC 2 cut(s) 499, 695
Bsp143I GATC 6 cut(s) 94, 185, 205, 502, 519, 583
BspACI CCGC 2 cut(s) 137, 784
BspANI GGCC 1 cut(s) 268
BspCNI CTCAG 2 cut(s) 713, 758
BspDI ATCGAT 2 cut(s) 188, 518
BspFNI CGCG 1 cut(s) 386
BspHI TCATGA 2 cut(s) 442, 736
BspLI GGNNCC 1 cut(s) 135
BspPI GGATC 3 cut(s) 193, 497, 578
BspTI CTTAAG 2 cut(s) 251, 284
BsrDI GCAATG 2 cut(s) 280, 675
BsrI ACTGG 4 cut(s) 383, 460, 478, 764
BssECI CCNNGG 2 cut(s) 48, 587
BssMI GATC 6 cut(s) 94, 185, 205, 502, 519, 583
BssNI GRCGYC 1 cut(s) 540
BssT1I CCWWGG 2 cut(s) 48, 587
Bst4CI ACNGT 4 cut(s) 35, 125, 697, 811
BstACI GRCGYC 1 cut(s) 540
BstAFI CTTAAG 2 cut(s) 251, 284
BstC8I GCNNGC 1 cut(s) 384
BstDEI CTNAG 2 cut(s) 700, 745
BstFNI CGCG 1 cut(s) 386
BstHHI GCGC 1 cut(s) 452
BstKTI GATC 6 cut(s) 97, 188, 208, 505, 522, 586
BstMAI GTCTC 3 cut(s) 201, 547, 703
BstMBI GATC 6 cut(s) 94, 185, 205, 502, 519, 583
BstMWI GCNNNNNNNGC 2 cut(s) 177, 790
BstNSI RCATGY 1 cut(s) 175
BstSFI CTRYAG 1 cut(s) 262
BstUI CGCG 1 cut(s) 386
BstV1I GCAGC 2 cut(s) 189, 618
BstV2I GAAGAC 2 cut(s) 126, 381
Bsu15I ATCGAT 2 cut(s) 188, 518
BsuI GTATCC 1 cut(s) 240
BsuRI GGCC 1 cut(s) 268
BsuTUI ATCGAT 2 cut(s) 188, 518
BtsIMutI CAGTG 4 cut(s) 130, 376, 552, 693
Cac8I GCNNGC 1 cut(s) 384
CciI TCATGA 2 cut(s) 442, 736
CfoI GCGC 1 cut(s) 452
Cfr13I GGNCC 2 cut(s) 134, 672
ClaI ATCGAT 2 cut(s) 188, 518
CseI GACGC 1 cut(s) 375
Csp6I GTAC 1 cut(s) 767
CviAII CATG 6 cut(s) 71, 172, 299, 443, 523, 737
CviJI RGCY 4 cut(s) 268, 497, 633, 731
CviKI_1 RGCY 4 cut(s) 268, 497, 633, 731
CviQI GTAC 1 cut(s) 767
DdeI CTNAG 2 cut(s) 700, 745
DpnI GATC 6 cut(s) 96, 187, 207, 504, 521, 585
DpnII GATC 6 cut(s) 94, 185, 205, 502, 519, 583
EciI GGCGGA 1 cut(s) 799
Ecl136II GAGCTC 1 cut(s) 497
Eco130I CCWWGG 2 cut(s) 48, 587
Eco147I AGGCCT 1 cut(s) 268
Eco24I GRGCYC 1 cut(s) 499
Eco47I GGWCC 2 cut(s) 134, 672
Eco53kI GAGCTC 1 cut(s) 497
Eco57I CTGAAG 2 cut(s) 102, 375
Eco88I CYCGRG 1 cut(s) 681
EcoICRI GAGCTC 1 cut(s) 497
EcoRI GAATTC 2 cut(s) 61, 477
EcoT14I CCWWGG 2 cut(s) 48, 587
EcoT38I GRGCYC 1 cut(s) 499
ErhI CCWWGG 2 cut(s) 48, 587
Esp3I CGTCTC 1 cut(s) 547
FaeI CATG 6 cut(s) 74, 175, 302, 446, 526, 740
FaqI GGGAC 2 cut(s) 147, 309
FatI CATG 6 cut(s) 70, 171, 298, 442, 522, 736
Fnu4HI GCNGC 2 cut(s) 178, 607
FriOI GRGCYC 1 cut(s) 499
Fsp4HI GCNGC 2 cut(s) 178, 607
FspBI CTAG 1 cut(s) 230
GlaI GCGC 1 cut(s) 451
GluI GCNGC 2 cut(s) 178, 607
HaeIII GGCC 1 cut(s) 268
HgaI GACGC 1 cut(s) 375
HhaI GCGC 1 cut(s) 452
Hin1I GRCGYC 1 cut(s) 540
Hin1II CATG 6 cut(s) 74, 175, 302, 446, 526, 740
Hin6I GCGC 1 cut(s) 450
HinP1I GCGC 1 cut(s) 450
HinfI GANTC 2 cut(s) 222, 743
HphI GGTGA 1 cut(s) 700
Hpy188I TCNGA 9 cut(s) 82, 99, 201, 214, 394, 547, 703, 725, 748
Hpy188III TCNNGA 5 cut(s) 443, 500, 670, 683, 737
HpyAV CCTTC 1 cut(s) 124
HpyCH4III ACNGT 4 cut(s) 35, 125, 697, 811
HpyCH4IV ACGT 1 cut(s) 540
HpyCH4V TGCA 9 cut(s) 74, 177, 193, 273, 329, 433, 654, 758, 793
HpyF10VI GCNNNNNNNGC 2 cut(s) 177, 790
HpyF3I CTNAG 2 cut(s) 700, 745
HpySE526I ACGT 1 cut(s) 540
Hsp92I GRCGYC 1 cut(s) 540
Hsp92II CATG 6 cut(s) 74, 175, 302, 446, 526, 740
HspAI GCGC 1 cut(s) 450
Kzo9I GATC 6 cut(s) 94, 185, 205, 502, 519, 583
LmnI GCTCC 3 cut(s) 168, 494, 502
Lsp1109I GCAGC 2 cut(s) 189, 618
LweI GCATC 2 cut(s) 180, 316
MaeI CTAG 1 cut(s) 230
MaeII ACGT 1 cut(s) 540
MaeIII GTNAC 3 cut(s) 234, 257, 469
MalI GATC 6 cut(s) 96, 187, 207, 504, 521, 585
MboI GATC 6 cut(s) 94, 185, 205, 502, 519, 583
MboII GAAGA 6 cut(s) 95, 104, 131, 381, 575, 716
MhlI GDGCHC 2 cut(s) 499, 695
MluCI AATT 4 cut(s) 61, 109, 477, 823
MluI ACGCGT 1 cut(s) 384
MlyI GAGTC 2 cut(s) 231, 737
MnlI CCTC 7 cut(s) 93, 109, 408, 421, 553, 679, 808
MseI TTAA 4 cut(s) 108, 252, 285, 344
MspCI CTTAAG 2 cut(s) 251, 284
MvnI CGCG 1 cut(s) 386
MwoI GCNNNNNNNGC 2 cut(s) 177, 790
NdeII GATC 6 cut(s) 94, 185, 205, 502, 519, 583
NlaIII CATG 6 cut(s) 74, 175, 302, 446, 526, 740
NlaIV GGNNCC 1 cut(s) 135
NmuCI GTSAC 2 cut(s) 234, 469
NspI RCATGY 1 cut(s) 175
PaeR7I CTCGAG 1 cut(s) 681
PagI TCATGA 2 cut(s) 442, 736
PceI AGGCCT 1 cut(s) 268
PflFI GACNNNGTC 1 cut(s) 221
PkrI GCNGC 2 cut(s) 179, 608
PleI GAGTC 2 cut(s) 230, 737
PpsI GAGTC 2 cut(s) 230, 737
Psp124BI GAGCTC 1 cut(s) 499
PspN4I GGNNCC 1 cut(s) 135
PspPI GGNCC 2 cut(s) 134, 672
PsyI GACNNNGTC 1 cut(s) 221
RsaI GTAC 1 cut(s) 768
RsaNI GTAC 1 cut(s) 767
SacI GAGCTC 1 cut(s) 499
SaqAI TTAA 4 cut(s) 108, 252, 285, 344
SatI GCNGC 2 cut(s) 178, 607
Sau3AI GATC 6 cut(s) 94, 185, 205, 502, 519, 583
Sau96I GGNCC 2 cut(s) 134, 672
SchI GAGTC 2 cut(s) 231, 737
SduI GDGCHC 2 cut(s) 499, 695
SetI ASST 6 cut(s) 419, 432, 499, 543, 690, 733
SfaNI GCATC 2 cut(s) 180, 316
SfcI CTRYAG 1 cut(s) 262
Sfr274I CTCGAG 1 cut(s) 681
SinI GGWCC 2 cut(s) 134, 672
SlaI CTCGAG 1 cut(s) 681
SmlI CTYRAG 3 cut(s) 251, 284, 681
SmoI CTYRAG 3 cut(s) 251, 284, 681
SpeI ACTAGT 1 cut(s) 229
Sse9I AATT 4 cut(s) 61, 109, 477, 823
SseBI AGGCCT 1 cut(s) 268
SsiI CCGC 2 cut(s) 137, 784
SspI AATATT 2 cut(s) 24, 463
SspMI CTAG 1 cut(s) 230
SstI GAGCTC 1 cut(s) 499
StuI AGGCCT 1 cut(s) 268
StyI CCWWGG 2 cut(s) 48, 587
TaaI ACNGT 4 cut(s) 35, 125, 697, 811
TaiI ACGT 1 cut(s) 543
TaqI TCGA 5 cut(s) 142, 188, 225, 518, 682
TasI AATT 4 cut(s) 61, 109, 477, 823
TatI WGTACW 1 cut(s) 766
Tru1I TTAA 4 cut(s) 108, 252, 285, 344
Tru9I TTAA 4 cut(s) 108, 252, 285, 344
TscAI CASTG 4 cut(s) 130, 383, 559, 700
TseFI GTSAC 2 cut(s) 234, 469
TseI GCWGC 2 cut(s) 177, 606
Tsp45I GTSAC 2 cut(s) 234, 469
TspDTI ATGAA 3 cut(s) 431, 611, 725
TspGWI ACGGA 1 cut(s) 363
TspRI CASTG 4 cut(s) 130, 383, 559, 700
Tth111I GACNNNGTC 1 cut(s) 221
Vha464I CTTAAG 2 cut(s) 251, 284
VpaK11BI GGWCC 2 cut(s) 134, 672
XapI RAATTY 2 cut(s) 61, 477
XceI RCATGY 1 cut(s) 175
XhoI CTCGAG 1 cut(s) 681
XspI CTAG 1 cut(s) 230
ZraI GACGTC 1 cut(s) 541
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.